Cumulative and persistent effects of nerve terminal depolarization on transmitter release. 1. Following focal depolarization of rat motor nerve terminals there could often be observed an ;after-discharge' of m.e.p.p.s with transient frequencies of up to 1000/sec. This after-discharge was graded with intensity and duration of the previous depolarization.2. Following pulses which were relatively short (about 1 sec) and not too large (< -100 mV local extracellular potential field) the logarithm of m.e.p.p. frequency fell exponentially. With larger or longer pulses there was a tail to the after-discharge which could persist for several minutes.3. M.e.p.p. frequency during an after-discharge was not inhibited appreciably by nerve terminal hyperpolarization, raised [Ca] (8 mM) or lowered pH.4. Measured as a multiplication of spontaneous m.e.p.p. frequency after-discharge was depressed in solution containing no Ca(2+) and added 1 mM-MgEDTA but equal in 0.125 mM-Ca(2+) or 2 mM-Sr(2+) to that in 2 mM-Ca(2+) or 8 mM-Ca(2+).5. During an after-discharge the multiplication of m.e.p.p. frequency by focal nerve terminal depolarization or raised K(+) was reduced. This phenomenon was termed ;uncoupling'.6. It was concluded that the after-discharge is not caused by a persistent rise of K(+) concentration in the synaptic cleft, nor by a maintained nerve terminal depolarization.7. In preparations depolarized by raised K(+) m.e.p.p. frequency during a relatively small focal depolarizing pulse rose continuously, after an initial rapid rise, and after the pulse there was a tail of increased m.e.p.p. frequency. The magnitude of the rise during the pulse and the tail after it were similar on, a logarithmic basis; during both processes the logarithm of m.e.p.p. frequency usually followed (approximately) an exponential time course.8. The relative magnitude of the slow effect of depolarization, as compared with the fast effect, was increased by lowering [Ca] or increasing [Mg], and the slow effect of depolarization in contrast to the fast effect was found to exist in the presence of Ca reduced to about 10(-7)M, but only during pulses. At this [Ca] there was no rapid response to depolarization. At [Ca] about 10(-10)M, there was no response at all of m.e.p.p. frequency to nerve terminal depolarization.9. The results are discussed, and compared with similar data referring to ;facilitation' and ;post-tetanic potentiation'. It is concluded that these and the slow effect of depolarization represent the same phenomenon, a response of the transmitter release system which can be distinguished from the fast response in terms of ionic requirement as well as time course.


The specific effect of potassium on transmitter release by motor nerve terminals and its inhibition by calcium. 1. There exist two distinct effects of potassium on the transmitter release system, one which develops rapidly and another which becomes maximal much more slowly. The fast effect is inhibited by raised Ca(2+), which does not inhibit transmitter release evoked by depolarizing pulses. Thus the fast effect is not secondary to nerve terminal depolarization.2. The fast effect of K(+) was found to consist in an increase in the slope of the linear relation between log m.e.p.p. frequency and nerve terminal depolarization. This effect is complete within a few seconds, is inhibited by raised Ca(2+), and is not produced by prolonged focal or electrotonic depolarization, which instead tends to reduce the slope of log m.e.p.p. frequency vs. depolarization.3. A slope change effect like that of K(+) was not found with ouabain or ethanol, nor did these agents depress the slope change effect of K(+). The specific action of K(+) was not exerted on release evoked in the absence of Ca(2+) by ethanol, chloral hydrate, or raised osmotic pressure.4. It is suggested that the specific action of K(+) is to increase the lability of nerve terminal Ca permeability with respect to depolarization of the nerve terminal membrane, while the slow effect of K(+) simply reflects nerve terminal depolarization, slow to become maximal because of diffusion barriers limiting access of raised K(+) to the Ranvier nodes of motor axons.


The role of calcium in depolarization-secretion coupling at the motor nerve terminal. 1. The relation between m.e.p.p. frequency (F) and [Ca] was studied at the mouse neuromuscular junction, at varied concentrations of K(+) and at nerve terminals depolarized by focal depolarization.2. Under all conditions the relation between log F and log [Ca] was sigmoid, with a maximum slope that increased with depolarization or raised [K(+)]. In addition, depolarization or raised K(+) caused a progressive shift of the sigmoid curve upward and to the left (to reduced log [Ca]) and increased the range over which log F could be altered by [Ca].3. Reduction of osmotic pressure changed the relation between log F and log [Ca] in the same way as increase of depolarization, while increase of osmotic pressure did the opposite.4. Raised [Mg] acted in two ways: (a) to shift the curve of log F vs. log [Ca] to the right and (b) to reduce maximum Delta log F/Delta log [Ca] without altering the range of log F sensitive to [Ca].5. The relation between log quantal content of e.p.p.s and log [Ca] was similar to that between log m.e.p.p. frequency and log [Ca].6. Individual nerve terminals varied in both Ca-dependent and Ca-independent fractions of log F; a large Ca-independent portion appears to be associated with a low Ca-dependent portion and vice versa. With large prolonged depolarization the Ca-independent portion was increased, apparently at the expense of the Ca-dependent portion.7. The results of all experiments were summarized in terms of parameters found by fitting the observed log release -log [Ca] curves to two theoretical equations, each derived on the basis of a model: (a) all-or-nothing activation of release probability by Ca-complex(es) and (b) graded activation of release probability by Ca complex(es).8. On the basis of the all-or-nothing model, from which follows alinear relation between F and amounts of Ca complex(es), the number of Ca(2+) atoms that ;cooperate' to mediate release appeared to increase progressively with presynaptic depolarization, to a value of 4 or more with a presynaptic action potential.9. On the basis of the graded activation model, which predicts an exponential relation between F and amount of Ca complex, the number of Ca(2+) atoms that combine with Ca receptor appears to be independent of presynaptic depolarization.10. Various models which could account for the data are discussed. It was concluded that all the data are consistent with a model in which:(i) quantal release probability is continuously graded with the amount of a simple Ca complex (CaX) inside the nerve terminal.(ii) Ca entry is governed by presynaptic membrane potential (increasing exponentially with depolarization) and by the amount of a Ca complex (Ca(2)Y) on or in the membrane.(iii) Mg(2+) competes with Ca(2+) at both receptors, X and Y.(iv) The internal Ca receptor X is also increased by presynaptic depolarization.


Melanotic neuroectodermal tumor of infancy.


Congenital cataract and ectopia lentis. An analysis of 152 patients treated in 1943-1967.


Age-dependent changes in the adenylate cyclase and phosphodiesterase activity of rat erythrocytes.


Scleroderma.

Scleroderma.


Dual viral infections in two dogs.

Dual viral infections in two dogs.


Cytochemical observations on glucose-6-phosphatase, glucosan phosphorylase, glucose-6-phosphate dehydrogenase, and  -glycerophosphate dehydrogenase in chick liver cell cultures infected with Trichomonas vaginalis.


A bovine herpesvirus associated with a disease of the upper respiratory tract of feedlot cattle.


ACTH and nasal polypi.

ACTH and nasal polypi.


The management of tumours of the glomus jugulare.


Plasma corticosterone level in runt disease.


Pathogenesis of Abelson-virus-induced murine leukemia.


Cultivation of cells from a fibroma in a rattlesnake, Crotalus horridus.


Activation in vitro, by 5-iododeoxyuridine, of a latent virus resembling C-type virus in a human sarcoma cell line.


Radioiodine-labeled antibody test for the detection of membrane antigens associated with Epstein-Barr virus.


Seroepidemiologic studies of herpesvirus type 2 and carcinoma of the cervix. II. Uganda.


Studies with human papilloma virus modeled after known papovavirus systems.


Mutation and cancer: a model for Wilms' tumor of the kidney.


Tumor angiogenesis: a quantitative method for histologic grading.


Immunologic and virologic studies of a nonproducer tumor induced by murine sarcoma virus (Harvey).


Enhancing effect of excess topical vitamin A on Rous sarcomas in chickens.


Inoculation of calves with particles resembling C-type virus from cultures of bovine lymphosarcoma.


Lung carcinoma of sheep (Jaagsiekte). 3. Lymph node, blood, and immunoglobulin.


Demonstration of Herpesvirus saimiri-associated antigens in peripheral lymphocytes from infected marmosets during in vitro cultivation.


Serologic evidence for a simian-virus-40-like infection of man.


Infiltrating duct carcinoma of the mammary gland of a rhesus monkey after administration of an oral contraceptive: a preliminary report.


Delayed hypersensitivity to simian virus 40 tumor cells in BALB-c mice demonstrated by a radioisotopic foot-pad assay.


Effect of vinblastine and colchicine on uptake and release of putative transmitters by synaptosomes and on brain actomyosin-like protein.


RNA and DNA in developing retinae: comparison of a normal with the degenerating retinae of C3H mice.


Metabolism of phosphatidylcholine, phosphatidylinositol and palmityl carnitine in synaptosomes from rat brain.


Metabolic consequences of ethanol oxidation in brains from mice chronically fed ethanol.


The measurement of the inorganic phosphate content of brain in the presence of bone fragments.


Accumulation of adenosine 3',5'-monophosphate in brain slices: interaction of local anaesthetics and depolarizing agents.


Activation of the pathway from locus coeruleus to rat cerebellar Purkinje neurons: pharmacological evidence of noradrenergic central inhibition.


Intrarenal prostaglandin release attenuates the renal vasoconstrictor activity of angiotensin.


Dynamics of binding and release of the polymyxin antibiotics by tissues.


Duration effects of supraliminal electrical stimuli to the tongue.


An alternative approach to generalized complementarity.


Effects of marijuana smoking on the sleep EEG. Preliminary studies.


Recovery of herpes-simplex virus from human trigeminal ganglions.


Tic douloureux: etiology, refined treatment.


Hypoexcitability of ulnar nerve in patients with normal motor nerve conduction velocities.


Further observations on subacute denervation and mammalian motor nerve terminal function.


Neuropsychologic and neurologic follow-up after herpesvirus hominis encephalitis.


Recurrence of nonneoplastic thymus after thymectomy for myasthenia gravis. Report of two cases.


Cytomegalovirus encephalitis in adults.

Cytomegalovirus encephalitis in adults.


Reduction of sympathetic outflow by central administration of L-DOPA, dopamine and norepinephrine.


Morphology of the normal liver cell.

Morphology of the normal liver cell.


Renal failure in advanced liver disease.


Hepatitis virology.

Hepatitis virology.


Lipoproteins and the liver.

Lipoproteins and the liver.


Red blood cell sodium and potassium concentrations in patients with liver diseases.


A method for the extraction, separation and estimation of unconjugated estrone, estradiol-17  and estradiol-17  in plasma.


The subcellular localization of the changes in ribonuclease and phosphatase activities of the mouse kidney produced by castration and testosterone.


Role of cyclic AMP and Ca ++  in mechanical and metabolic events in isometrically contracting vascular smooth muscle.


Down's syndrome and malignancy.

Down's syndrome and malignancy.


Human brain and platelet cyclic adenosine 3',5'-monophosphate phosphodiesterases: different response to drugs.


Adenovirus pneumonia.

Adenovirus pneumonia.


An outbreak of adenovirus type 7 infection in children in Montreal. Thirteen infants and children with adenovirus type 7 infection proved by virus isolation are described. High fever, cough and dyspnea were the most frequent findings; in infants under 1 year of age wheezing was common. Four patients required artificial ventilation. Lobar collapse, consolidation and hyperinflation were frequent radiologic findings. None of the symptoms responded to antibiotic therapy or bronchodilator drugs. Three patients died (mortality rate of 23%). Pathologic findings were compatible with adenovirus type 7 pneumonia, and were characterized by a necrotizing bronchitis and bronchiolitis, patchy alveolar fibrinopurulent exudate and hyaline membrane formation. Some intra-alveolar epithelial cells showed strikingly abnormal nuclei and rare typical halo-outlined intranuclear inclusions were seen. Only one of eight survivors had evidence of significant chronic chest disease.


The molecular basis of mutagenesis by methyl and ethyl methanesulfonates.


Regulation of yeast inorganic-pyrophospphatase activity by divalent cations.


Antineoplastic and immunosuppressive drugs. II. Therapeutic uses.


Phosphate transfer between 2,3-biphosphoglycerate and monophosphoglycerate catalyzed by bisphosphoglycerate phosphatase.


The substrate specificity of exonuclease A5.


Studies of glutamate dehydrogenase. Characterization of histidine residues involved in the activity and association. Photoactivated labelling with pyridoxal 5'-phosphate.


Studies of glutamate dehydrogenase. Modification with 5-diazo-1H-tetrazole and glyoxal. Importance of two different amino groups for the binding of 2-oxoglutarate and NADH.


Biosynthesis of N-acetylneuraminic acid in Morris hepatomas.


Inhibition of mitochondrial-protein synthesis in rat small-intestinal epithelium.


Reciprocal induction and repression of serine dehydratase and phosphoglycerate dehydrogenase by proteins and dietary-essential amino acids in rat liver.


Measurement of plasma renin concentration (PRC) using exogenous substrate and radioimmunoassay.


Calcium and phosphorus metabolism parathyroid hormone, calcitonin and bone histology in pseudohypoparathyroidism.


The response to TRH, LH-RH, metyrapone and vasopressin in patients with hypothalamo-pituitary disorders.


Column chromatographic determination of polymyxin B sulfate.


Perspectives on policy research.

Perspectives on policy research.


The significance of dense core particles in subacute demyelinating disease in an adult.


Auer bodies in acute promyelocytic leukemia. Demonstration of their fine structure and peroxidase localization.


Thoracic duct drainage during lymphangiography.


Dynamic anatomy of the ulnar nerve at the elbow.


Vascular patterns in cerebral neoplasms and their differential diagnosis.


The ten most common types of brain tumor.


Cystectomy and segmental resection for primary carcinoma of the bladder: experience at Ochsner Clinic 1942-1968.


The microbial ecology of the activated sludge process.


Respiratory infections.

Respiratory infections.


A current look at feline lymphoma.

A current look at feline lymphoma.


Coincidental pulmonary granular cell schwannoma and bronchogenic adenocarcinoma.


Distinctive cytopathic properties of cytomegalovirus recovered from the female genital tract.


Mallory bodies in a case of primary biliary cirrhosis: an ultrastructural and morphogenetic study.


The dependence of pancuronium- and d-tubocurarine-induced neuromuscular blockades on alveolar concentrations of halothane and forane.


Adrenocorticoids and RNA metabolism in kidney. I. Effect of adrenalectomy on RNA turnover in various subcellular fractions of rat kidney.


Stimulation by reticulocyte initiation factors of protein synthesis in a cell-free system from Krebs II ascites cells.


Effect of in vitro histone phosphorylation on template activity of rat liver chromatin.


Synchronous cultures of Chlamydomonas reinhardti. Synthesis of repressed and derepressed phosphatase during the life cycle.


Partial purification of cortisol-binding protein from rat liver cytosol and its role in transcription.


Inclusions in human lens capsule and their relationship to senile exfoliation.


Isolation of cytochrome c abundant mutant of Candida utilis.


Use of the Du Pont "automatic clinical analyzer" in direct determination of lactic acid in plasma stabilized with sodium fluoride.


Radioimmunoassay of serum renin activity and digoxin concentrations, with use of polyethylene glycol to separate free and antibody-bound ligand.


Use of 3-methyl-2-benzothiazolone hydrazone in an automated colorimetric method for determination of triglycerides in plasma or serum.


Speeded simultaneous turbidimetry of cholesterol in  - and pre- -lipoproteins.


Descending supraspinal effect in tetanus intoxication of the spinal cord.


Invertebrate central neurons: orthograde degeneration and retrograde changes after axonotomy.


Biochemistry of adenosine triphosphatase activity of avian fast and slow muscle.


Hyperpolarization of trigeminal primary afferents evoked by stimulation of lumbar sympathetic trunk and peripheral nerves.


Ongoing activity in peripheral nerve: interactions between electrical stimulation and ongoing activity.


Allozyme genotype-environment relationships. I. Variation in natural populations of Drosophila pachea. Remarkable geographic and seasonal uniformities in gene and genotype frequencies were found for four enzyme loci. Principal component analysis revealed two patterns of allele frequency changes in three of the allozyme loci (Est-b, Est-c and Acph) and one pattern in the Mdh-d locus. These patterns accounted for 90% to 100% of the variability at these loci. Significant positive correlations were obtained between several of the components and the variability in the non-saponifiable and alkaloid fractions of the senita cactus host plant. The genetic variance within a single locality (Guaymas, Mexico) was as great as the variance between all localities sampled. This is interpreted as a local founder effect. The temporary nature of the rotting cactus breeding site, the delayed maturation and the differential maturation rate of females and males are three factors proposed to cause forced outbreeding and counter the local founder effect. A combination of selection resulting from a direct interaction of the genotype and the environment, in addition to migration, is proposed to maintain the uniform gene frequencies.


Mechanisms of the reactions of cytochrome c. II. The rate of reduction of horse-heart ferricytochrome c by chromium(II).


Reactions of monomeric cobalt-oxygen complexes. I. Thermodynamics of reaction of molecular oxygen with five- and six-coordinate amine complexes of a cobalt porphyrin.


Use of vanadyl(IV) ion as a new spectroscopic probe of metal binding to proteins. Vanadyl insulin.


Cyclic adenosine 3',5'-monophosphate-dependent protein kinase stimulation of calcium uptake by canine cardiac microsomes.


Adrenocorticotropins. 44. Total synthesis of the human hormone by the solid-phase method.


An electron spin resonance study of radicals produced in the photolysis of  -keto acids and esters.


Behavior of lambda bacteriophage in nicotinamide adenine dinucleotide deficient Escherichia coli cells.


Further simplification of the serum neutralization test with herpes simplex virus.


Various tests in the evaluation of islet cell adenomas.


Oxytocin analogs in the analysis of some phases of hormone action.


Estrogen action: an inroad to cell biology. The literature concerning the mechanism of estrogen action is extensively reviewed and a concept of the mechanism is proposed.  2 recurrent observations in the studies in this area are: 1) cells responsive to estrogen contain a recepter protein (or recepter aggregate) that interacts specifically with structural features of the e strogen molecule forming a high  affinity, noncovalent complex and 2) many of the hormonal responses are dependent on hormonal acceleration of the genetic expression mechanisms in the sensitive cells.  A theory is presented in which estrogen is visualized as a modulator in the class expression of multiple genes through the control of the availability or state of proteins essential in the nuclear scene for expression of these genes.  The conceptual framework provides for both the variation in estr ogenic responses in various target organs.  Following the main body of the article Mueller discusses the concept and related considerations with serveral other researchers.


Structure, synthesis, and mechanism of action of parathyroid hormone.


18-Hydroxy-11-deoxycorticosterone (18-OH-DOC) secretion in experimental and human hypertension.


Autoregulation of renal blood flow in rabbits immunized with angiotensin II.


Angiotensin. II. Important role in the maintenance of arterial blood pressure. An angiotensin II antagonist, [1-sarcosine, 8-alanine]-angiotensin II, was given intravenously to anesthetized dogs with thoracic caval constriction and ascites to investigate the role of angiotensin II in the control of arterial pressure. The antagonist produced a striking fall in arterial pressure and in aldosterone secretion and an accompanying increase in plasma renin activity. In a control experiment, normal anesthetized dogs were given the angiotensin analog, but it failed to reduce arterial pressure or to influence plasma renin activity. In conscious dogs with caval constriction, the antagonist produced essentially the same drop in arterial pressure as observed in anesthetized animals. These results suggest an important role for angiotensin II in the maintenance of arterial pressure by its action on specific receptor sites in arteriolar smooth muscle and in the adrenal cortex.


Norepinephrine-containing neurons: changes in spontaneous discharge patterns during sleeping and waking. Norepinephrine-containing neurons of the locus coeruleus of the cat were recorded with microelectrodes during unrestrained sleeping and waking. The recorded neurons were subsequently defined by combined fluorescence histochemistry of catecholamines and production of microlesions at recording sites. These pontine units show homogeneous changes in discharge patterns with respect to sleep stages, firing slowly during drowsy periods and slow wave sleep and firing in rapid bursts during paradoxical sleep. These data provide a direct correlation between the activity of defined catecholamine-containing neurons and the spontaneous occurrence of sleep stages.


Proton beam radiography in tumor detection. Monoenergetic protons are highly sensitive to density variations and are capable of giving radiographs of very high contrast. As an initial step in exploring their diagnostic potential, protons with energies of 160 million electron volts are used in "contact radiography" on tumor-bearing human brain specimens. The proton radiographs are compared with x-radiographs taken under identical specimen conditions.


Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B. BSC-1 monkey kidney cells were enucleated by two cycles of centrifugation in the presence of cytochalasin B. The resulting populations contained 99.5 percent enucleated cells. After infection, newly synthesized poliovirus was recovered from the enucleated populations. Final virus yield per enucleated cell was about one-fifth the yield per infected untreated cell


Cylic AMP. A mediator of hormone response and its clinical application.


Changes in net hydration of DNA during thermal helix-coil transition.


New federal legislation on alcoholism--opportunities and problems.


Assay for the intactness of the outer membrane in isolated mitochondria.


Interconvertible and noninterconvertible forms of rat pyruvate kinase.


Characterization of two forms of beef heart cyclic nucleotide phosphodiesterase.


Xanthine oxidase catalyzed oxidation of aldehydes. Oxidation of aliphatic aldehydes and 2- and 4-pyridinecarboxaldehyde.


Effect of spermidine on the activity of DNA polymerases.


Terminal nucleotides of avian myeloblastosis virus RNA and of ribosomal RNA from chicken leukemic myeloblasts.


Retroperitoneal xanthogranuloma and xanthosarcoma (malignant fibrous xanthoma).


Some systemic effects of malignant tumors. I. Co-enzyme A levels.


Leiomyomatous hamartoma of the kidney. A clinical and pathologic analysis of 20 cases from the Kidney Tumor Registry.


Intrahepatic bile duct carcinoma and C. sinensis infection in Hong Kong.


Heterogeneity of human serum high-density lipoprotein (HDL 2 ).


The amino acid sequence of bat (Miniopteris schreibersi) cytochrome C.


Effect of morphine on bradykinin-induced unitary discharges in the spinal cord of the rabbit.


The use of estrogen antagonists in hormone receptor studies.


A critique on the evaluation and mode of action of antiestrogens.


Estrogens and antiestrogens.

Estrogens and antiestrogens.


Effect of antiestrogens on uterine estradiol receptors.


Oestrogen uptake by the central nervous system.


Studies on carcinoembryonic antigen.

Studies on carcinoembryonic antigen.


Aspects on the mode of action of antiestrogens and antiprogestogens.


Changes in 5-hydroxytryptamine metabolism during anaphylactic shock in rats.


Adenosine 3',5'-cyclic monophosphate in Chlamydomonas reinhardtii. Influence on flagellar function and regeneration. Adenosine 3',5'-cyclic monophosphate (cAMP) influences both flagellar function and flagellar regeneration in Chlamydomonas reinhardtii. The methylxanthine, aminophylline, which can cause a tenfold increase in cAMP level in C. reinhardtii, inhibits flagellar movement and flagellar regeneration by wild-type cells, without inhibiting cell multiplication. Caffeine, a closely related inhibitor, also inhibits flagellar movement and regeneration, but it inhibits cell multiplication too. Regeneration by a mutant lacking the central pair of flagellar microtubules was found to be more sensitive than wild type to inhibition by caffeine and to be subject to synergistic inhibition by aminophylline plus dibutyryl cAMP. Regeneration by three out of seven mutants with different flagellar abnormalities was more sensitive than wild type to these inhibitors. We interpret these results to mean that cAMP affects a component of the flagellum directly or indirectly, and that the responsiveness of that component to cAMP is enhanced by mutations which affect the integrity of the flagellum. The component in question could be microtubule protein.


Lysosomal phospholipase A 1  and A 2  of normal and bacillus calmette guerin-induced alveolar macrophages. A single intravenous injection of 0.1 mg of heat-killed Bacillus Calmette Guérin (BCG) in 0.1 ml of Bayol F produced an accumulation of activated alveolar macrophages (BCG induced). Cells were collected 3.5-4.0 wk after injection. Phospholipases A and three lysosomal marker enzymes (acid phosphatase, beta-glucuronidase, and lysozyme) were measured in homogenates, and the distribution of the phospholipases A and lysosomal, mitochondrial, and microsomal marker enzymes were examined after sucrose gradient centrifugation of a postnuclear (1,000 g) supernatant. Homogenates of normal and BCG-induced macrophages contained phospholipases A(1) and A(2) which had optimal activity at pH 4.0 in the presence of 2.0 mM ethylenediaminetetraacetate (EDTA). These activities were inhibited 50-70% by 2.0 mM CaCl(2). Homogenates of BCG-induced macrophages had specific activities of beta-glucuronidase, acid phosphatase, and lysozyme, which were increased 1.5- to 3.0-fold over the controls, whether expressed as activity per mg protein or activity per 10(7) cells. The specific activities of the phospholipases A, on the other hand, were consistently lower than those of the control. Distribution of the phospholipases A and the lysosomal marker enzymes after sucrose gradient centrifugation suggested that the phospholipases A active at pH 4.0 in the presence of EDTA are of lysosomal origin since: (a) BCG treatment caused a selective increase in the density of particles which contained both the phospholipases A and three lysosomal marker enzymes; and (b) since the density of mitochondria and microsomes were not affected by BCG treatment. The increase in the density of lysosomes seen here may be related to previously described morphologic changes of BCG-induced alveolar macrophages.


Evidence for pleiotropic changes in lines of Chinese hamster ovary cells resistant to concanavalin A and phytohemagglutinin-P. Lines of Chinese hamster ovary cells resistant to the lectins concanavalin A (Con A) and phytohemagglutinin-P (PHA-P) have been isolated and characterized. Lines were isolated by a stepwise, a single-step, or a cycling single-step procedure, from both mutagen-treated and untreated cultures. The resistant lines showed a higher efficiency of colony formation in the presence of the appropriate lectin than did the wild-type parental line. The cell lines resistant to Con A did not exhibit any detectable cross resistance to PHA-P, nor did the PHA-resistant cells exhibit cross resistance to Con A. The toxicity of Con A from the wild-type and Con A-resistant lines was reduced in the presence of methyl alpha-D-glucopyranoside; this effect was not seen with the PHA-resistant line. Using (125)I-labeled Con A, it was found that Con A was bound preferentially to the surface of intact cells, and that the amount of labeled Con A bound to intact cells was similar for the wild-type and lectin-resistant lines. The Con A-resistant lines were found to be more susceptible to the toxic effects of a number of different compounds, including cyclic AMP and its dibutyryl derivative, sodium butyrate, high concentrations of glucose, phenethyl alcohol, phenol, ouabain, and testosterone. It appears that, in these lines, acquisition of resistance to Con A gave rise to pleiotropic effects which were detected by changes in the sensitivity of the cells to a variety of agents.


The effects of secretagogues on the incorporation of (2- 3 H)myoinositol into lipid in cytological fractions in the pancreas of the guinea pig in vivo. Stimulation of enzyme secretion in the pancreas on injection of a single dose of the cholinergic drug, pilocarpine, was associated with an increased incorporation of [2-(3)H]myoinositol into a lipid, which was previously characterized as phosphatidylinositol. Stimulation of enzyme secretion by hourly injection of the pancreozymin congener, caerulein, led to more increased phosphatidylinositol synthesis than with a single injection of pilocarpine. The amylase level of the pancreas remained at a low level as long as caerulein was injected, indicating continued stimulation of enzyme secretion even though increased phosphatidylinositol synthesis ceased after 6 h. Feeding gave the same stimulation of phosphatidylinositol synthesis as caerulein. The major synthesis of phosphatidylinositol in controls and the stimulation of phosphatidylinositol synthesis by pilocarpine was entirely confined to the microsome fraction throughout the experiments (up to 18 h). This shows that there is no flow of microsomal membrane (smooth- or rough-surfaced endoplasmic reticulum) to other membranous structures throughout the secretory cycle and beyond. It is concluded that the stimulation of phosphatidylinositol synthesis by pancreatic secretagogues is confined to microsomal elements and does not play any role in membrane flow.


Fine structure of nerve fibers and growth cones of isolated sympathetic neurons in culture. The leading tips of elongating nerve fibers are enlarged into "growth cones" which are seen in tissue culture to continually undergo changes in conformation and to foster numerous transitory slender extensions (filopodia) and/or a veillike ruffling sheet. After explantation of 1-day-old rat superior cervical ganglia (as pieces or as individual neurons), nerve fibers and tips were photographed during growth and through the initial stages of aldehyde fixation and then relocated after embedding in plastic. Electron microscopy of serially sectioned tips revealed the following. The moving parts of the cone, the peripheral flange and filopodia, contained a distinctive apparently filamentous feltwork from which all organelles except membranous structures were excluded; microtubules were notably absent from these areas. The cone interior contained varied forms of agranular endoplasmic reticulum, vacuoles, vesicles, coated vesicles, mitochondria, microtubules, and occasional neurofilaments and polysomes. Dense-cored vesicles and lysosomal structures were also present and appeared to be formed locally, at least in part from reticulum. The possible roles of the various forms of agranular membranous components are discussed and it is suggested that structures involved in both the assembly and degradation of membrane are present in the cone. The content of these growing tips resembles that in sensory neuron growth cones studied by others.


Expression of the mitochondrial genome in HeLa cells. IX. Effect of inhibition of mitochondrial protein synthesis on mitochondrial formation. The effect of selective inhibition of mitochondrial protein synthesis by chloramphenicol at 40 or 200 microg/ml on the formation of mitochondria in HeLa cells was investigated. HeLa cells, under the conditions used in the present work, grow at a decreasing rate for at least four cell generations in the presence of 40 microg/ml chloramphenicol, and for two generations in the presence of 200 microg/ml chloramphenicol. The progressive cell growth inhibition which begins after 2 days of exposure of the cells to 40 microg/ml chloramphenicol is immediately or gradually reversible, upon removal of the drug, for periods up to at least 8 days of treatment, though there is a progressive loss of cloning efficiency. In cells which have been treated for 6-7 days with 40 or 200 microg/ml of chloramphenicol, mitochondrial protein synthesis occurs at a normal or near-normal rate 1 h after removal of the drug. Mitochondria increase normally in number and show a normal size and amount of cristae in the presence of either concentration of drug. However, in 4-5% of the mitochondrial profiles the cristae appear to be arranged in unusual, circular, looped or whorled configuration.


Mode of mitochondrial formation in HeLa cells.


Interferon: effect on the saturation density to which mouse cells will grow in vitro.


A labyrinthine structure formed from a transverse tubule of mouse ventricular myocardium.


Phenobarbital-induced, membrane-like scrolls in the oenocytes of Musca domestica linnaeus.


Influence of niacinamide administration on brain 5-HT and a possible mode of action.


Angiotensin effect on uptake of norepinephrine by synaptosomes.


The effects of acetaldehyde on the kinetics of the energy-dependent phosphate-induced swelling of rat liver mitochondria.


Potency disturbances following Abrodil myelography.


Nursing care study; carcinoma of the stomach complicated by metastases.


Vitamin C and respiratory infections.

Vitamin C and respiratory infections.


Toxic effect of the fruit of Passiflora adenopoda D. C. on humans: phytochemical determination.


The phosphorylation of 3'-deoxy-3'-fluorothymidine and its incorporation into DNA in a cellfree system from tumor cells.


Study on the intracellular mechanism of LH release in the anterior pituitary.


Diagnosis and management of insulinoma.

Diagnosis and management of insulinoma.


Quantitative gas chromatographic analysis of amino acids on a short glass capillary column.


Release of aminoacyl transfer ribonucleic acid from transfer ribonucleic acid synthetase studied by rapid molecular sieve chromatography.


Fractionation of microbial DNA strands on poly I-coated kieselguhr.


Clinical course and complications in Hodgkin's disease.


The binding of hydrogen and calcium ions by Tamm-Horsfall glycoprotein.


A study of the cystine-containing apolipoprotein of human plasma high density lipoproteins: characterization of cyanogen bromide and tryptic fragments.


Backbone folding of immunoglobulin light and heavy chains: a comparison of predicted  -bend positions.


The effect of compound F6066 on rat luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release in vitro.


Distribution and binding of J 131 -human chorionic gonadotropin (HCG) in different organs of pseudopregnant female rats.


Ultraviolet-sensitive mutator mutU4 of Escherichia coli inviable with polA. Attempts to transduce the ultraviolet-sensitive mutator lesion mutU4 into strains deficient in deoxyribonucleic acid polymerase I (polA) were unsuccessful. Mutator recombinants were found when the polA recipient had first been reverted to Pol(+) by selection for resistance to methyl methanesulfonate. The inviability of the mutU4 polA double mutant was demonstrated by a reduction in the absolute number of transductants when the recipient was polA as compared with Pol(+), and selection was made for markers very close to mutU4. Double mutants containing mutU4 and polA4, which determines a cold-sensitive polymerase, were unable to grow at 24 C, the nonpermissive temperature.


Spin-labeling studies on the membrane of a facultative thermophilic bacillus. Spin-labeling studies were conducted to elucidate the rigidity and viscosity of the membrane of a facultative thermophilic bacillus. By employing nitroxide stearate probes, it was observed that this fatty acid was incorporated into the membrane fraction. Electron spin resonance spectroscopy indicated that cells grown at 55 C contained a membrane which was more rigid and viscous than the membrane from cells grown at 37 C.


A new crystal-containing cell in human adrenal cortex. Electron microscope examination of the adrenal cortex from three male human subjects revealed a special type of cell occurring in periendothelial spaces, in all adrenal cortex zones. It is a clear, spindle-shaped cell the principal cytoplasmic features of which are crystalline inclusions with a structure similar to that of the Reinke crystals of human testicular interstitial cells and an abundance of microfilaments. Enzymatic digestions with pronase, pepsin, and ribonuclease were performed, and no digestion of the crystals was obtained. The crystals had no peroxidase or acid phosphatase activities. This cell appears to be exclusive to human males and it may be related to adrenal androgen secretion.


Comparative transport activity of intact cells, membrane vesicles, and mesosomes of Bacillus licheniformis. Sodium ion was shown to stimulate strongly the transport of l-glutamic acid into cells of Bacillus licheniformis 6346 His(-). Lithium ion had a slight capacity to replace Na(+) in this capacity, but K(+) was without effect. Three of five amino acids tested. l-glutamic acid, l-aspartic acid, and l-alanine, were concentrated against a gradient in the cells. Intracellular pools of these amino acids were extractable with 5% trichloroacetic acid. Pools of l-histidine and l-lysine could not be detected. No evidence of active transport of lysine into cells could be detected, and histidine was taken up in the absence of chloramphenicol but not in its presence. The uptake of glutamic acid by membrane vesicle preparations was strongly stimulated by reduced nicotinamide adenine dinucleotide (NADH) and to a lesser extent by succinate. The presence of phenazine methosulfate increased uptake in the presence of succinate. Either l- or d-lactate and adenosine triphosphate were without effect. None of these compounds stimulated the uptake of glutamic acid by mesosomes, although some mesosome preparations contained separable membrane which was very active. NADH strongly stimulated the uptake of aspartic acid and alanine by membrane vesicles but had only a slight effect on the uptake of histidine and lysine. No evidence of active transport of any of the amino acids into mesosomes could be detected either in the presence or absence of NADH. NADH stimulation of the uptake of glutamic acid by membrane vesicles was destroyed by exposure to light of 360 nm; this inactivation was reversible by vitamin K(2(5)) or K(2(10)). Sodium ion stimulated transport of glutamic acid by membrane vesicles.


Thymidine transport by cultured Novikoff hepatoma cells and uptake by simple diffusion and relationship to incorporation into deoxyribonucleic acid. The initial rate of thymidine-(3)H incorporation into the acid-soluble pool by cultured Novikoff rat hepatoma cells was investigated as a function of the thymidine concentration in the medium. Below, but not above 2 microM, thymidine incorporation followed normal Michaelis-Menten kinetics at 22 degrees , 27 degrees , 32 degrees , and 37 degrees C with an apparent K(m) of 0.5 microM, and the V(max) values increased with an average Q(10) of 1.8 with an increase in temperature. The intracellular acid-soluble (3)H was associated solely with thymine nucleotides (mainly deoxythymidine triphosphate [dTTP]). Between 2 and 200 microM, on the other hand, the initial rate of thymidine incorporation increased linearly with an increase in thymidine concentration in the medium and was about the same at all four temperatures. Pretreatment of the cells with 40 or 100 microMp-chloromercuribenzoate for 15 min or heat-shock (49.5 degrees C, 5 min) markedly reduced the saturable component of uptake without affecting the unsaturable component or the phosphorylation of thymidine. The effect of p-chloromercuribenzoate was readily reversed by incubating the cells in the presence of dithiothreitol. Persantin and uridine competitively inhibited thymidine incorporation into the acid-soluble pool without inhibiting thymidine phosphorylation. At concentrations below 2 microM, thymidine incorporation into DNA also followed normal Michaelis-Menten kinetics and was inhibited in an apparently competitive manner by Persantin and uridine. The apparent K(m) and K(i) values were about the same as those for thymidine incorporation into the nucleotide pool. The over-all results indicate that uptake is the rate-limiting step in the incorporation of thymidine into the nucleotide pool as well as into DNA. The cells possess an excess of thymidine kinase, and thymidine is phosphorylated as rapidly as it enters the cells and is thereby trapped. At low concentrations, thymidine is taken up mainly by a transport reaction, whereas at concentrations above 2 microM simple diffusion becomes the principal mode of uptake. Evidence is presented that indicates that uridine and thymidine are transported by different systems. Upon inhibition of DNA synthesis, net thymidine incorporation into the acid-soluble pool ceased rapidly. Results from pulse-chase experiments indicate that a rapid turnover of dTTP to thymidine may be involved in limiting the level of thymine nucleotides in the cell.


Modifications of lincomycin involving the carbohydrate portion. II. Analogues with D-gluco- and D-ido-stereochemistry.


Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells. Cytochalasin B competitively inhibits the transport of uridine and thymidine by Novikoff rat hepatoma cells growing in suspension culture with apparent K(i)'s of 2 and 6 microM, respectively, but has no effect on the intracellular phosphorylation of the nucleosides. Choline transport is not affected by cytochalasin B. Results from pulse-chase experiments indicate that cytochalasin B has no direct effect on the synthesis of RNA, DNA, or uridine diphosphate-sugars. The inhibition of uridine and thymidine incorporation into nucleic acids by cytochalasin B is solely the consequence of the inhibition of nucleoside transport.


The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro. The in vitro interaction of soluble horseradish peroxidase (HRP) with homogeneous mono layers of mouse macrophages has been studied using sensitive biochemical and cytochemical techniques. The compartmentalization of HRP in extracellular and intracellular sites has been quantitatively evaluated. A significant fraction is bound to a serum-derived layer, which coats the surface of culture vessels and may be removed by appropriate washes. Macrophages interiorize HRP as a solute in pinocytic vesicles without appreciable binding of the glycoprotein to the plasma membrane. Uptake is directly proportional to the concentration of HRP in the culture medium. 1 x 10(6) cells ingest 0.0025% of the administered load per hr over a wide range of concentrations. Cytochemically, all demonstrable HRP is sequestered within the endocytic vesicles and secondary lysosomes of the vacuolar apparatus. After uptake, the enzymatic activity of HRP is inactivated exponentially with a half-life of 7-9 hr, until enzyme is no longer detectable. When macrophages have pinocytosed trace-labeled HRP-(125)I, cell-associated isotope disappears with a t (1/2) of 20-30 hr and they release monoiodotyrosine-(125)I into the culture medium. We were unable to obtain evidence that significant amounts of HRP (>2%) can be exocytosed after uptake, can exist intact on the cell surface, or can be digested extracellularly. It is difficult to reconcile these observations with several of the postulated mechanisms whereby macrophages are thought to play a prominent role in the induction of an immune response.


Effect of adenosine 3'-5'-cyclic monophosphate on cell proliferation. Secondary cultures of human diploid fibroblasts, which demonstrate density-dependent inhibition of cell growth, were used to study the effect of adenosine 3'-5'-cyclic monophosphate (cAMP) on cell proliferation. DNA synthesis in nonconfluent cultures and in contact-inhibited cultures stimulated to grow by refeeding with fresh medium was found to be inhibited by exogenous cAMP. The properties of this inhibition of DNA synthesis, together with the alterations in cAMP metabolism observed in confluent cultures of cells stimulated with fresh medium to resume growth, strongly suggest that cAMP is involved in contact-inhibition of cell proliferation.


Effect of catecholamine analogs on control of actomyosin by native tropomyosin and calcium: the nature of the negative inotropic receptor.


Studies of the presynaptic effect of  -bungarotoxin on neuromuscular transmission.


Inhibition of adrenergic neurotransmission by parasympathomimetics in the rabbit ear artery.


Vasoconstriction produced by hallucinogens on isolated human and sheep umbilical vasculature.


-Foetoprotein in rats with hepatomas induced by aflatoxin B 1 .


Suppression of pituitary-adrenal response to stress with physiological plasma levels of corticosterone in the female rat.


Eczema herpeticum and pregnancy.

Eczema herpeticum and pregnancy.


Clear cell adenocarcinoma in young women.


Respiratory responses to ACTH and dexamethasone in unanesthetized goats.


Proinsulin.

Proinsulin.


Possible implications for medicine of RNA-directed DNA synthesis.


Nuclear magnetic resonance studies of the dynamic aspects of molecular structure and interaction in biological systems.


On the development of amino acid transport systems.


Elicitation of periods of inhibition in human muscle by stimulation of cutaneous nerves.


En bloc resection of the shoulder girdle: technique and indications. Report of a case.


Scaphoid and lunate dislocation. Report of a case in a patient with peripheral neuropathy.


An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses. Chick, hamster, mouse, and rat embryo fibroblast cultures, transformed by either DNA or RNA viruses, show fibrinolytic activity under suitable conditions of growth and in appropriate media; normal counterpart cultures do not. The fibrinolysin is produced by the interaction of two protein factors: one of these, a cell factor, is released by transformed cells and accumulates in the medium when cultures are incubated in the absence of scrum. The second factor, the serum factor, is a specific protein that is present in sera of many avian and mammalian species, including man. Not all sera yield fibrinolysin on interaction with any given transformed cell factor, and the spectrum of activating sera is distinctive for each cell factor. This pattern appears to be determined by the cell type, rather than by the transforming virus. An important role for the fibrinolysin in oncogenic transformation is suggested by the following correlations. (a) The initial appearance of fibrinolysin precedes the morphological change after the transfer to permissive temperatures of chick fibroblast cultures infected with a temperature-sensitive mutant of RSV. (b) The initiation of fibrinolysis and of morphological change both require the synthesis of new protein, but not the synthesis of either DNA or rRNA. (c) The activity of the fibrinolysin is correlated with the retention of abnormal morphology in hamster cells transformed by SV-40. (d) The sera of normal chicks effectively activate fibrinolysis with the cell factor from transformed chick cells. In contrast the sera of chicks with RSV tumors do not; these contain an inhibitor of the fibrinolytic activity.


A pharmacological analysis of the response of dog anterior mesenteric vein to transmural electrical stimulation.


Epstein-Barr virus. Heterophile responses in squirrel monkeys inoculated with virus-transformed autologous leukocytes. Epstein-Barr virus (EBV)-transformed autologous lymphoblasts were repeatedly inoculated into three squirrel monkeys. Each animal developed the heterophile antibodies of infectious mononucleosis and EBV-specific antibodies. After serologic responses had disappeared or markedly declined, the animals were challenged with either whole cells, cell filtrate, or cell ghosts. Animals challenged with living cells and cell ghosts developed agglutinin responses; the recipient of filtrate did not. The results suggest that EBV induces the appearance of the infectious mononucleosis heterophile antigen on the transformed cell membrane.


An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses. Chick embryo fibroblast cultures develop fibrinolytic activity after transformation by Rous sarcoma virus (RSV). This fibrinolytic activity is not present in normal cultures, and it does not appear after infection with either nontransforming strains of avian leukosis viruses or cytocidal RNA and DNA viruses. In cultures infected with a temperature sensitive mutant of RSV the onset of fibrinolysis appears after exposure to permissive temperatures and precedes by a short interval the appearance of morphological evidence of transformation. See PDF for Structure The rate of fibrinolysis in transformed cultures depends on the nature of the serum that is present in the growth medium: some sera (e.g., monkey or chicken serum) promote high enzymatic activity, while others (calf, fetal bovine) do not. Some sera contain inhibitors of the fibrinolysin. Based on the effect of a small number of known inhibitors, at least one step of the fibrinolytic process shows specificity resembling that of trypsin. The sera of sarcoma-bearing chickens contain an inhibitor of the fibrinolysin, whereas normal chicken sera do not. For general discussion, conclusions, and summary see the accompanying paper, part II, (J. Exp. Med. 137:112).


Hormonal changes in ewes and their fetuses at parturition.


Small-angle x-ray scattering of human serum high-density lipoproteins.


Effect of folic acid on plasma renin activity in rats.


Treatment of myasthenia gravis.

Treatment of myasthenia gravis.


Unsuspected tuberculosis in a cancer patient.


Pseudohypohyper what?

Pseudohypohyper what?


The clinical utility of brainscanning in nuclear medicine.


Prostaglandin (PGA1), angiotensin and renal function.


Studies in familial (medullary) thyroid carcinoma.


Tektite II: medical supervision of the scientists in the sea. XIV. Biochemical, hematological, and endocrine studies.


Diagnostic problems in adenoviral disease.


Clinical appraisal of an inactivated bovine rhinotracheitis/parainfluenza-3 vaccine with pasteurella bacterin.


Functional regulation of mammalian ribonucleotide reductase.


New aspects of polyamine biosynthesis in eukaryotic organisms.


Mode of action of specific inactivating enzymes for pyridoxal enzymes and NAD-dependent enzymes and their biological significance.


Two carbamyl phosphate synthetases of mammals: specific roles in control of pyrimidine and urea biosynthesis.


Regulatory characteristics of lactate dehydrogenases.


Isozyme studies on adult, regenerating, precancerous and developing liver in relation to findings in hepatomas.


Correlations between clinical and laboratory findings in virus infection of the female genital tract.


Skin test for amebiasis: an appraisal.

Skin test for amebiasis: an appraisal.


Intrauterine electroencephalogram of the sheep fetus.


Guillain-Barré syndrome. Increased lymphoproliferative potential.


Chronic herpes simplex virus infection. Initiation in hamsters upon implantation of infected nonpermissive glial cells.


The central nervous system in Fabry's disease. An ultrastructural study.


Scanning electron microscopic study of skeletal muscle. Normal, destrophic, and neurogenic atrophic muscle in mice and humans.


Myotonia induced by monocarboxylic aromatic acids. A possible mechanism.


Prolonged paralysis in pseudocholinesterase deficiency.


Polymyositis with intranuclear inclusions. A light and electron microscopic study.


Metabolism of I-hydroxy-2-ketoheptadecane in myelinating brain.


Incorporation of 2-deoxy-D-glucose into glycolipids of normal and SV40-transformed hamster cells.


Strain L fibroblast lipases. Purification and properties.


Growth of establishes lymphocytes in selective media: application to studies of inborn metabolic errors and somatic mutations.


The role of Epstein-Barr virus in establishing long-term human lymphocyte cultures.


Methodology for cultured human lymphoid cells.


Biohazards of long-term lymphocyte cultures.


Spontaneous and induced transformation of human leukocytes in vitro.


The lymphocyte in culture: historic perspective.


Herpesvirus hominis infection in patients with myeloproliferative and lymphoproliferative disorders. Infection with Herpesvirus hominis, often associated with oral ulceration, was found to be more frequent in patients with myeloproliferative and lymphoproliferative disorders than in normal populations or patients with other diseases. This increased frequency was not associated with any deficiency of the humoral antibody response, suggesting a possible impairment of cell-mediated immunity. The otherwise untreatable oral lesions appeared to respond effectively to local irradiation.


Transient cranial mononeuropathy.

Transient cranial mononeuropathy.


Terminal oxidations in Bacillus brevis. Soluble reduced nicotinamide adenine dinucleotide oxidase activity in Bacillus brevis.


Effects of postovulatory estrogen on progesterone and prostaglandin F levels in the monkey.


Multiplication of Mycobacterium lepraemurium in cell-free medium containing  -ketoglutaric acid and cytochrome c.


Conidiophore and spore development in Aspergillus nidulans.


The levels of cyclic AMP during substrate-accelerated death.


Nurses as multiple therapists.

Nurses as multiple therapists.


Teaching nurses to care for the dying patient.


Megavitamins: pro and con.

Megavitamins: pro and con.


The nurse-therapist: a staff nurse position which emphasizes clinical practice.


Effects of vitamin E on toxicoderma associated with liver damage. Case report.


The American Association of Retired Persons.


Leadership and social change in 1973?

Leadership and social change in 1973?


Phasing out a state mental hospital.

Phasing out a state mental hospital.


The role of the nurse in crisis intervention and suicide prevention.


Viewing the past as prologue for changes today and tomorrow.


Studies on the site of renal salt loss in a patient with Bartter's syndrome.


Gerontological nursing: where the action is.


Thiamine deficiency in adult hospital patients.


Models of copper-protein interaction: the crystal structure of (glycyl-L-histidylglycinato)-copper (II) sodium perchlorate hydrate.


Histochemistry of hydroxysteroid dehydrogenases in the testis, adrenocartical tissue, and corpuscles of Stannius of Pseudopleuronectes americanus.


Journal's culpa?

Journal's culpa?


Reciprocal inhibitary effect of 5-iodo-2'-deoxyuridine and thymidine into the cells infected with herpes simplex virus.


Phenelzine, mood response, and sleep.

Phenelzine, mood response, and sleep.


Cholinergic activation of vestibular neurones leading to rapid eye movements in the mesencephalic cat.


Sphingolipids of mushrooms.

Sphingolipids of mushrooms.


Temperature-dependent binding of estrogen receptor to chromatin.


Affinity labeling of Escherichia coli B deoxyribonucleic acid dependent ribonucleic acid polymerase.


A random sequential mechanism for arginyl transfer ribonucleic acid synthetase of Escherichia coli.


Control of lipid metabolism in hepatomas: conversion of glutamate carbon to fatty acid carbon via citrate in several transplantable hepatomas.


A facile synthesis of 1-acyl ethylene glycol-2-phosphorylcholines.


Glycerophosphoryl diglucosyl diglyceride, a new phosphoglycolipid from Streptococci.


Role of high density lipoproteins in the lecithin-cholesterol acyltransferase activity with sonicated lecithin-cholesterol dispersions as substrate.


The kinetic properties of very low density lipoprotein triglyceride in type 3 hyperlipoproteinemia.


Molecular forms of enzymes in cancer.

Molecular forms of enzymes in cancer.


Glycogen storage diseases.

Glycogen storage diseases.


The use of premarin, conjugated estrogens-equine to control. Functional uterine bleeding and the urinary estrogen metabolism after administration.


Levels of cytochromes in heart, liver, kidney and brain in the developing rat.


Hyperpolarization by cyclic AMP (activation of diphosphoinositide kinase).


Induction of brain tumours in high yield by administration of N-ethyl-N-nitrosourea to newborn rats.


Effect of N 6 O 2 -dibutyryl cyclic 3',5'-adenosine monophosphate on the pinocytosis of brain capillaries of mice.


Interferon production by Sendai virus-treated hamster and monkey cells.


Biological properties of synthetic Ser 4 -Arg 8 -oxytocin (Ile 3 -Ser 4 -arginine vasopressin): role of the residue No. 4 in the hormone-pressor receptor interaction.


Compensatory growth of the ovaries, adrenal glands and kidneys in blinded, anosmic rats.


Plasma NADase in tuberculosis.

Plasma NADase in tuberculosis.


Correlation between changes in antitumor immune parameters and tumor growth in vivo in rats.


An analysis of dishabituation and sensitization of the gill-withdrawal reflex in Aplysia.


Recurrent excitation in the CA3 region of cat hippocampus.


Diurnal rhythms of sleep in the rat: augmentation of paradoxical sleep following alterations of the feeding schedule.


Synthesis of a fully protected heptacosapeptide norleucine analog corresponding to positions 21-47 of the primary structure of staphylococcal nuclease.


Studies on the coordination of reduced glutathione to B 12  coenzymes.


Common peroneal nerve palsy. Evaluation and management.


Medical beliefs and practices of the Aymara indians.


LSD and cyanotic congenital heart disease.


The treatment of toxoplasmosis during pregnancy.


Pseudo-fi + I-like sex factors, R62(I), selective for increased pilus synthesis. An I-like R factor, R62(I), inhibits F-like pilus synthesis and selects for mutant sex factors with a greatly increased capacity for pilus production.


Differential block at high frequency of branches of a single axon innervating two muscles.


An electron spin resonance study of nitrosamine anion radicals.


Transformation of rat liver cells with chicken sarcoma virus B77 and murine sarcoma virus. Rat liver cells in vitro were transformed with chicken sarcoma virus B77, giving RL(B77) cells, and with murine sarcoma virus (Harvey), giving RL(MSV) cells. Rat liver cells transformed spontaneously in vitro were designated RL cells. In addition, the RL(MSV) cell line was adapted for growth in culture fluid containing 25 mug of 5-bromodeoxyuridine per ml. All cell lines were tumorigenic in 1-wk-old rats. The number of cells needed for induction of tumor growth was 1,000-fold higher in the case of RL(B77) cells in comparison with RL(MSV) cells and RL cells. No production of viral particles from any of the cell lines investigated was detected by plating concentrated supernatant fluid of the cultures on different secondary embryo cells with and without fusion by Sendai virus, by labeling with uridine-5-(3)H, or by assay for deoxyribonucleic acid polymerase activity. The viral genome was rescued by fusion of RL(B77) cells with chicken cells. Chicken sarcoma virus rescued from (RL(B77) cells differed in plating efficiency on duck cells from B77 virus rescued from transformed rat embryo cells. No virus was rescued after fusion of RL(MSV) and RL cells with mouse, rat, or chicken embryo cells. Infectious murine sarcoma virus can be induced by 5-bromodeoxyuridine from RL(MSV) cells.


Stimulation of aryl hydrocarbon hydroxylase induction in cell cultures by interferon. In cell cultures previously treated with homologous interferon, the magnitude of antiviral activity and the degree of stimulation of aryl hydrocarbon hydroxylase induction appear to be directly related. In a highly purified mouse interferon preparation, the factor stimulating hydroxylase induction and the factor directing antiviral activity are inactivated by heating to 70 C or by treating with trypsin. Also, both activities demonstrate species specificity.


Extraordinary effects of specific monovalent cations on activation of reovirus transcriptase by chymotrypsin in vitro. Activation of reovirus transcriptase activity, latent in intact virions, by digestion of purified virions with chymotrypsin (CHT) in vitro shows a stringent requirement for specific monovalent cations. Cs(+), Rb(+), or K(+) ions are capable of facilitating activation by chymotryptic digestion. Na(+), Li(+), or NH(4) (+) ions are not capable of facilitating the CHT activation of polymerase activity and are antagonistic towards the effects of the facilitating ions. The data indicate that the effect of the cations is exerted on activation of the polymerase activity by CHT as opposed to an effect on polymerization per se. This effect may be important biologically in that it provides a mechanism whereby the virion can sense whether it is in an intracellular or an extracellular environment and thereby can avoid premature uncoating.


Effect of viral double-stranded RNA on protein synthesis in intact cells. The addition of purified, noninfectious, double-stranded RNA of bovine enterovirus, a picornavirus, to intact cells in culture results in a rapid cessation of cellular polypeptide synthesis. This inhibition is specific for host cell protein synthesis since the translation of picornavirus-specific proteins is not affected by the double-stranded viral RNA.


Characterization of morphologic revertants of murine and avian sarcoma virus-transformed cells. Morphologic revertants which contain avian or murine sarcoma viruses have previously been isolated at low frequency from clonal lines of transformed mammalian cells. In the present study, these lines have been further characterized. They are indistinguishable from nontransformed parent cell lines with respect to parameters such as saturation density and colony formation in depleted medium or on monolayers of contact-inhibited cells. The rate of glucose uptake had also reverted to normal. The malignant potential of one of the revertant lines was examined and found to be markedly reduced compared to that of the corresponding transformed cells. The differences in the susceptibilities of revertant cells to retransformation by the same or other oncogenic viruses suggest that different cellular genes may be involved in expression of transformation by various tumor viruses.


Stimulation of Sendai virion transcriptase by polyanions. Exogenous polyribonucleotides stimulated the ribonucleic acid (RNA) transcriptase in Sendai virions. Added yeast RNA, polyadenylic acid, or polycytidylic acid increased incorporation of (3)H-guanosine monophosphate as much as fivefold. The products of stimulated reactions were virus-specific as determined by hybridization with Sendai virion RNA, but they sedimented more slowly (13s) than the product of an unstimulated reaction (16s). The stimulating activity was nondialyzable and heat stable, but was abolished by alkaline hydrolysis. Nucleoside monophosphates, individually or in combination, were ineffective, confirming the requirement for a polymer. Among other substances tested for effects on Sendai virion transcriptase, polyaspartic acid and polyglutamic acid stimulated the enzyme; polyinosinic acid, polyuridylic acid, and polyamines had no effect; and dextran sulfate and polyvinyl sulfate were inhibitory.


Isolation of an infectious ribonucleoprotein from vesicular stomatitis virus containing an active RNA transcriptase. A ribonucleoprotein complex (TNP) containing an active RNA polymerase was isolated from purified vesicular stomatitis virus particles. The TNP sedimented through a sucrose gradient as a single band and appeared under the electron microscope as discrete long filaments in a spiral configuration. TNP contained one major and two minor polypeptides, but not the polypeptides associated with the outer coat of vesicular stomatitis virus. BHK-21 clone 13 cells could be infected with TNP, yielding infectious virus particles.


In vivo conversion of the single-stranded DNA of the kilham rat virus to a double-stranded form. Kilham rat virus (KRV) contains linear, single-stranded DNA in the virion. The fate of radioactive viral DNA was followed after infection of monolayer cells. Within 60 min after infection of cells, 28 to 42% of the parental viral DNA is converted to a new form. This new DNA form is believed to be double stranded and linear on the basis of its sedimentation in neutral and alkaline sucrose gradients, elution from hydroxyapatite columns, its buoyant density in equilibrium CsCl density gradients, and appearance in the electron microscope. The double-stranded linear KRV DNA may be analogous to the replicative form of certain bacteriophages, including phiX174, which contain single-stranded circular genomes.


Chromatographic and electrophoretic analysis of viral proteins from hamster and chicken cells transformed by Rous sarcoma virus. Several methods have been explored for the detection and characterization of viral proteins from soluble extracts of cells transformed by Rous sarcoma virus (RSV). Viral antigens have been analyzed after gel filtration in several solvents. In addition, immune complexes formed with virus-specific sera have been isolated by agarose gel filtration and by high- or low-speed centrifugation through sucrose solutions. Radioactive proteins from these immune complexes have been analyzed by gel filtration in 6 m guanidine hydrochloride or by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Comparison with proteins from purified virus indicates the presence of two viral core proteins (gs1 and gs2) in the soluble fraction from virus-producing chicken cells. In the same fraction from RSV-transformed hamster cells (which do not produce virus), three gs proteins (gs1, gs2, and gs3) could be identified. The soluble viral gs proteins are strongly bound to at least two larger polypeptides in cell extracts. These polypeptides do not appear to be viral in origin and have the property of undergoing a time-dependent aggregation in the extracts. One of these cell-derived proteins, which is present in a variety of uninfected cell types, closely resembles actin.


Picornaviral gene order: comparison of a rhinovirus with a cardiovirus. We describe conditions for unmasking virus-specific protein synthesis in cells infected with rhinoviruses 1A and 2. The gene order and cleavage pattern of rhinovirus 1A show many similarities to those of encephalomyocarditis virus and of poliovirus.


Role of mitochondria in the production of RNA-containing tumor viruses. The role of mitochondria in the reproduction of RNA-containing tumor viruses was examined by using ethidium bromide (EB) to induce degenerative effects in mitochondria. The effects of EB in murine and avian cells were monitored by electron microscopy. Chronically infected mouse (JLS-V5) cells, in which extensive mitochondrial changes were induced, continued to produce murine leukemia virus. Also, complete reproductive cycles of Rous sarcoma virus (RSV) occurred in newly infected chicken embryo cells exposed to EB. Morphological transformation characteristic of infection of chicken embryo cells by RSV occurred in cells which contained induced aberrant mitochondria. The results demonstrate that mitochondria play a relatively minor role, if any, in the reproduction of RNA-containing tumor viruses.


A note on the duration of neutralizing antibody against avian encephalomyelitis virus in chickens.


The fine structure of Marek's disease virus and herpesvirus of turkey in cell culture.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 15-1973.


The possibilities of applying mass miniature radiography for public health care.


The role of mass chest radiography in preventive medicine.


Conservative mediastinal node dissection for the treatment of carcinoma of the breast.


Experiences with bilateral primary carcinoma of the breast.


Effect of chlorothiazide and sodium on vascular responsiveness to angiotensin II.


Changes in renin kinetics induced by nephrectomy.


Effects of ganglionic blocking agents on adrenergic transmission in rat mesenteric arteries.


Hydration changes produced by central infusion of angiotensin II.


Metabolic clearance rate of radioiodinated angiotensin II in normal men.


Enterostomal therapy: better care for the ostomy patient.


Biochemical and morphological aspects of zinc deficiency in Rhodotorula gracilis.


Enzyme induction and repression in Arthrobacter crystallopoietes.


Regulation of the synthesis of mitochondrial enzymes and cytochromes. Distinction between catabolite repression and anaerobiosis in Saccharomyces cerevisiae.


Chemical composition of gas vesicles isolated from Anabaena flos-aquae.


Impression-expression reactions in cellular pathology.


Cobaltous ion complex of reduced lipoamide dehydrogenase.


Glycoproteins associated with nuclei of cells before and after transformation by a ribonucleic acid virus.


On the active site topography of isoleucyl transfer ribonucleic acid synthetase of Escherichia coli B.


Amino acid incorporation and products of protein synthesis in isolated chloroplasts of Euglena gracilis.


Hormonal effects on thymidine kinase and thymidylate kinase activity of estrogen-dependent tumors in the rat.


Ultrastructural effects of 5-bromodeoxyuridine and 5-fluorouracil in Drosophila.


Activities and isoenzyme patterns of some enzymes of glucose metabolism in human primary malignant hepatoma.


The carcinogenic potential of aflatoxin or its metabolites in rats from dams fed aflatoxin pre- and postpartum.


Ultrastructural, histological, and biochemical alterations produced by 12-O-tetradecanoyl-phorbol-13-acetate on mouse epidermis and their relevance to skin tumor promotion.


Inhibition of transfer and ribosomal RNA methylases by polyinosinate.


Transplantability and biological behavior of mouse liver tumors induced by ethylnitrosourea.


Cyclic adenosine 3',5'-monophosphate phosphodiesterase activity in normal, differentiating, regenerating, and neoplastic liver.


Reversible and irreversible effects of tissue culture on enzyme patterns of spontaneous mouse tumors and mouse and human embryo tissues.


Dihydroorotate dehydrogenase in liver and Morris hepatomas.


Enhanced responses to tyramine and angiotensin produced by 4,4'-biphenylene bis-((2-oxoethylene)-bis-(2,2-diethoxylethyl)dimethylammonium bromide) (DMAE).


Selective release of newly synthesized cardiac norepinephrine induced by angiotensin II.


Angiotensin-induced contraction of the cat nictitating membrane.


The effect of a hemicholinium analogue, HC-15, of neuromuscular transmission.


Cellular analysis of immunosuppression to synthetic polypeptide immunogens induced by a murine leukemia virus.


The physicochemical characteristics and purification of slow-reacting substance of anaphylaxis.


Variations in complement levels during active and adoptive immunization.


Inactivation of human interferon by urine.


Twice weekly tuberculosis chemotherapy.

Twice weekly tuberculosis chemotherapy.


Synaptic delay and time course of postsynaptic potentials at the junction between hair cells and eighth nerve fibers in the goldfish.


Neonatal sepsis due to clostridium perfringens.


The hyperlipoproteinemias. Detection, diagnosis, and management.


Granular cell tumours of the larynx.

Granular cell tumours of the larynx.


SV40 virus transformation of mouse 3T3 cells does not specifically enhance sugar transport. The apparent enhancement of 2-deoxy-D-glucose uptake by mouse 3T3 cells accompanying transformation by SV40 virus is not due primarily to an effect on the transport process but to enhanced phosphorylation of the sugar by intracellular kinases. Moreover, the effect is not specifically a function of the presence of the viral genome, but is a reflection of the overall growth rate and physiological state of the cell.


Urethroplasty for anterior urethral stricture.


The syndrome of hypertension, hyperreninemia and secondary aldosteronism associated with renal juxtaglomerular cell tumor (primary reninism).


The cancer associated antigen test as an index to failure of complete removal of urological cancers.


Plan and operation of the health and nutrition examination survey. United states--1971-1973.


Increasing antibody titers to herpes simplex virus type 2 during follow-up of women with cervical dysplasia.


Prevalence of antibodies to Epstein-Barr virus, cytomegalovirus and Toxoplasma in a Mexican highland community.


Incidence and etiology of pneumonia, croup and bronchiolitis in preschool children belonging to a prepaid medical care group over a four-year period.


Viral and mycoplasmal pneumonia in a prepaid medical care group during an eight-year period.


Protamine kinase independent of adenosine 3',5'-monophosphate from rat brain cytosol.


Cyclic AMP and citric acid accumulation by Aspergillus niger.


RNase and alkali sensitivity of closed circular mitochondrial DNA of rat ascites hepatoma cells.


Comparative binding of albumin and  -glucuronidase by rat liver microsomes.


Carbon tetrachloride activation in liver microsomes from rats induced with 3-methylcholantrene.


The terminal oxidases of Azotobacter vinelandii.


Differences in the carbon tetrachloride-induced damage to components of the smooth and rough endoplasmic reticulum from rat liver.


The role of cyclic AMP in the phosphorylation of proteins in human erythrocyte membranes.


UDP-glucose pyrophosphorylase synthesis in myxamoebae of Dictyostelium discoideum.


Studies of the Rous sarcoma virus RNA: characterization of the 5'-terminus.


UDP-glucose dehydrogenase: substrate binding stoichiometry and affinity.


4,4'-Bis dimethylaminodiphenylcarbinol: a new reagent for selective chemical modification. Interaction with porcine malate dehydrogenase.


The effect of inhibitors of RNA and protein synthesis on dibutyryl cyclic AMP mediated morphological transformations of Chinese hamster ovary cells in vitro.


EPR behavior of a soluble cardiac NADH dehydrogenase.


The primary acid product of DPNH.

The primary acid product of DPNH.


Modification of hamster sperm adenyl cyclase by capacitation in vitro.


Role of  -NADH for microsomal ethanol oxidation.


Release and binding of proteins and enzymes with isolated inner mitochondrial membranes.


The kinetics of the cytochrome-c-azurin redox equilibrium.


Charge distribution in electron transport components: cytochrome c and cytochrome c oxidase mixtures.


Activation of glycogen synthetase and inactivation of phosphorylase kinase by the same phosphoprotein phosphatase.


Acetylcholine stimulates hydrolysis of  32 P-labeled phosphatidic acid in guinea pig synaptosomes.


New poliovirus-related polypeptides in the nucleus-associated fraction of HeLa cell cytoplasm.


Biosynthesis of the parasporal inclusion of Bacillus thuringiensis: half-life of its corresponding messenger RNA.


Oestradiol "neo nuclear receptor" in the rat uterus: sedimentation constant and conditions of formation.


Central pleomorphic adenoma in the mandible: report of a case.


Method for determination of cyclic AMP in plasma.


Stable reagents for determination of serum triglycerides by a colorimetric Hantzsch condensation method.


Specificity of leucine stimulation of insulin release.


Effects of eserine and neostigmine on release of acetylcholine into perfused cerebral ventricles of unanaesthetized dogs.


Effect of parainfluenza-3 neuraminidase on bovine nasal secretion. Three samples of bovine nasal secretion were each separated into a sol phase and a surface gel phase. In all samples, the gel phase contained an approximately four times greater amount of bound N-acetylneuraminic acid (NANA) than the sol phase. From the gel phase, bound NANA could be released by exposure to parainfluenza-3 virus neuraminidase. The surface gel appears to be a natural substrate for this enzyme.


Semisynthetic  -lactam antibiotics. 1. Acylation of 6-aminopenicillanic acid with activated derivatives of  -sulfophenylacetic acid.


Antimetabolites of coenzyme Q. 17. Improved synthesis of 5-hydroxy-1,4-benzoquinone analogs and their indices.


Design, synthesis, and broad spectrum antiviral activity of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide and related nucleosides.


Potential antitumor agents. 6. Possible irreversible inhibitors of ribonucleoside diphosphate reductase.


Potential antifertility agents. 2. Tetrazole derivatives of nonsteroidal estrogens.


Interplay of vasodilator and vasoconstrictor substances in the femoral arterial bed: identification of the angiotensin cofactor.


Alterations of rat liver lysosomes and smooth endoplasmic reticulum induced by the diazafluoranthen derivative AC-3579. I. Morphologic and biochemical lesions.


Effect of AY-9944, a cholesterol biosynthesis inhibitor, on fetal lung development and on the development of type II alveolar epithelial cells.


What's your diagnosis? Adenocystic carcinoma.


Amnesia, consolidation, and retrieval.

Amnesia, consolidation, and retrieval.


Metabolism of glucose in the human endometrium with special reference to fertility and contraception.


Purine overproduction in man associated with increased phosphoribosylpyrophosphate synthetase activity. In hemolyzates from red cells of two brothers with purine overproduction and gout, activity of phosphoribosylpyrophosphate synthetase is more than twofold greater than that measured in normal or other gouty individuals. The increased enzyme activity, which is also demonstrable in fibroblasts of the one patient tested, is associated with increased production of 5-phosphoribosyl-1-pyrophosphate by intact cells, an indication that the enzyme abnormality is the basis for the purine overproduction. This genetic abnormality is an example of an increased enzyme activity producing a disease state.


Fibrin degradation products during postoperative radiotherapy of ovarian carcinoma.


The number of glomerular cells and substructures in early juvenile diabetes. A quantitative electron microscopic study.


Metastases to the uterus. Five cases diagnosed on the basis of curettings.


Sexual precocity attributable to ectopic gonadotropin secretion by hepatoblastoma.


The possible role of the carotid bodies as chemoreceptors in the laryngeal reflex.


On a collagenase-like enzyme detected in the extracts of hypothalamus and hypophysis.


Composition of low density lipoprotein in children with familial hyperbetalipoproteinaemia and the effect of treatment.


A reproducible quantitative collagenase radiofibril assay.


Electroimmunodiffusion of   2 M, IgA and IgM in nanogram quantities with a hydroxyethylcellulose-agarose gel: application to unconcentrated CSF.


Regulatory processes in the maturation and early cleavage of amphibian eggs.


Studies on the human glomerular basement membrane. Composition, nature of the carbohydrate units and chemical changes in diabetes mellitus.


Sensitivity of the electron spin resonance technique as applied in histochemical research on normal and pathological calcified tissues.


Dynamics of axial ligation in metalloporphyrins. II.  Porphyrin "inversion" and halogen exchange in high-spin iron complexes.


Electron spin resonance of (25 Mg)chlorophyll a.


The future for medical education and women in medicine.


Pitfalls in the diagnosis of amebic colitis.


Rehabilitation medicine as an art form.

Rehabilitation medicine as an art form.


Medical education. One step at a time.

Medical education. One step at a time.


The radiologic aspects of toxoplasmosis.


Paget's disease of the anus secondary to a malignant papillary adenoma of the rectum.


Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues. A radioimmunoassay specific for a murine leukemia virus structural protein, the gs antigen, detects an antigenic reactivity in normal murine cells in culture and natural tissues. The assay was shown to measure an antigen that is highly related to the virion protein as shown by absorption tests, immunoadsorbent chromatography, and by analysis of linearized dose-response curves. These findings combined with the finding of viral-specific RNA indicate that portions of the viral genome are being expressed with a much greater frequency than previously appreciated.


Prevention of cell-to-cell spread of herpes simplex virus by leukocytes. Antibody to herpes simplex virus (HSV) plus complement destroyed HSV-infected cells but did not stop the spread of the infection. Studies on the relationship between the time of appearance of viral antigens on the cell surface, immunological destruction of the cells by antiviral antibody and complement, and transfer of the virus to adjacent cells showed that the virus spread from infected to uninfected cells before the infected cells were susceptible to immunological destruction. Incubation of infected monolayers with leukocytes, however, stopped the spread of the virus by nonspecifically damaging both infected and uninfected cells and by presumably breaking intercellular bridges. When leukocytes were removed from infected monolayers, viral plaques developed. If, however, antiviral antibody and complement were added to monolayers before the leukocytes were removed, the development of plaques was prevented. These findings suggest that both antibody and leukocytes are needed to cure HSV infections.


Immunologic rejection of diethylnitrosamine-induced hepatomas in strain 2 guinea pigs: participation of basophilic leukocytes and macrophage aggregates. The morphologic events associated with the immunologic rejection by strain 2 guinea pigs of ascites variants of two lines of diethylnitrosamine-induced tumors have been studied by light and electron microscopy. Tumor injection sites in the skin of control animals exhibited clusters of viable, actively mitotic tumor cells along with a modest inflammatory infiltrate composed of lymphocytes, macrophages, neutrophils, and rare basophils. In contrast, similar injections of either tumor line in specifically sensitized guinea pigs elicited typical delayed-type skin reactions associated with tumor cell necrosis and a more extensive inflammatory infiltrate including a selective increase in the number of basophilic leukocytes (12%, line 1, or 23%, line 10, of total inflammatory cells). That basophils may have a role in tumor resistance in vivo is suggested by the close anatomic associations observed between basophils and tumor cells, and by the fact that basophils were the only inflammatory cell to demonstrate a relative increase in frequency in the lesions of sensitized as compared with control animals. Moreover, intraperitoneal injection of line 1 tumor in specifically sensitized animals elicited a striking basophilia within 24 h. Unlike macrophages, basophils did not phagocytose tumor cells but did evidence occasional extrusion of granules and frequently exhibited loss of granule staining density, a change that may be related to release of mediator substances. Electron microscope studies of line 1 tumor rejection in the peritoneal cavities of specifically sensitized guinea pigs demonstrated aggregations of "activated" macrophages, lymphocytes, basophils, and damaged or dead tumor cells. These aggregates, held together by complex interdigitations of macrophage villi, closely resembled those occurring in vitro among peritoneal exudate cells whose migration from capillary tubes was inhibited by migration inhibition factor (MIF). Moreover, cells in these aggregates, as well as macrophages inhibited by MIF in vitro, lacked a normal coating of cell surface material.


Accelerated hypertension secondary to oral contraceptives.


Bone as a target organ: toward a better definition of osteoporosis.


Distribution of tritiated human chorionic gonadotropin in superovulated rat ovary.


Cyclic 3',5'-AMP phosphodiesterase activity during cyclic AMP-induced differentiation of neuroblastoma cells in culture.


Differentiation of a myocardial depressant factor present in shock plasma from known plasma peptides and salts.


Microheterogeneity of N-acetyl- -D-hexosaminidase of bull epididymis.


Interaction of prostaglandin E 1  and angiotensin II on centrally mediated pressor activities in the cat.


Interferon induced by endotoxin and Newcastle disease virus in rabbit macrophage and kidney cell cultures.


Biochemical events in murine cytomegalovirus-infected mouse liver.


Renal medullary adenylate cyclase in drug-induced nephrogenic diabetes insipidus.


Alpha-ecdysone and 20-hydroxyecdysone from frass of the tobacco hornworm, Manduca sexta (L.).


Characterization of the metabolites of vitamin D 3  in the chick.


Biochemistry of the hydroxyprolines.

Biochemistry of the hydroxyprolines.


Parainfluenza virus Sendai infection in macrophages, ependyma, choroid plexus, vascular endothelium and respiratory tract of mice. Immunofluorescent observations showed that after intranasal instillation of parainfluenza 1 (Sendai) virus into adult mice, infection is confined to the epithelial lining of the larger airways. Alveolar macrophages were not significantly involved, although they could be infected in vitro. In suckling mice, the infection was more acutely lethal and extended into the terminal air spaces. The intranasal susceptibility of adult mice was not reproducibly affected by treatment with potent antithymocyte serum, and there were no obvious pathogenic effects when heterologous antiserum was instilled intranasally into infected mice. Peritoneal macrophages were infected by intraperitoneally injected Sendai virus, with production of a highly viscous peritoneal exudate. Kupffer cells of the liver and endothelial cells in large veins and auricles were infected by intravenously injected virus. When injected intracerebrally, Sendai virus infected ependyma and choroid plexus epithelium. Adult mice often survived, in spite of ependymal destruction and changes in ventricular morphology. Astrocytes were activated but not infected.


The Hermansky-Pudlak syndrome: ultrastructure of bone marrow macrophages. The present investigation has explored the fine structure of the lipid inclusions which fill the bone marrow macrophages of patients with the Hermansky-Pudlak syndrome. Red blood cells are the major substrate of the reticular macrophages, and incomplete digestion of erythrocytes leads to formation of the massive inclusions. Progressive transformation of the macrophages results in an end-stage cell whose damaged cytoplasm is nearly replaced by huge lipid-containing vacuoles surrounded by particulate debris. This type of cell has not been found in bone marrows from patients with hemolytic, thrombocytopenic or lipid storage diseases.


Action of quinacrine mustard on polynucleotides.


Studies in bioluminescence. VII. Bacterial NADH: flavin mononucleotide oxidoreductase.


Inhibition by sulfonamides of the photoreductive step in spinach chloroplast photosynthesis.


Familial recurrent polyneuropathy. A family and a survey.


Aspects of subcortical organization of feeding revealed by hypothalamic disconnexions in Macaca mulatta.


Proteolytic activity of a small variety of Entamoeba histolytica. II. Detection of enzyme and effect of pH on its activity.


Cytomegalovirus syndrome post-renal transplantation. Treatment with cytosine arabinoside.


Thecoma in pregnancy.

Thecoma in pregnancy. A description is briefly given of a patient whose pregnancy was complicated by an ovarian thecoma which ruptured spontaneously.Eleven previously reported instances of the combination of thecoma and pregnancy are summarized.Although the present case appears to be the first in which the mother has survived rupture of the tumour, in view of the result to the child this can hardly be claimed as a triumph of obstetrics.


Hypersomnia in manic-depressive disease (a preliminary report).


Clinical neuropathological conference.

Clinical neuropathological conference.


Mechanism of acupunctural anesthesia. The two-gate control theory.


Separation of methylated bases of ribonucleic acid by two-dimensional thin-layer chromatography.


Liquid chromatographic analysis of nucleosides, purine base-modified adenosine derivatives and 3',5'-cyclic AMP.


Increased mycoplasma-cell association in transformed human amnion cells.


Failure of cytarabine in varicella-zoster infections.


Treatment of labial herpes.

Treatment of labial herpes.


Herpetic chancre.

Herpetic chancre.


Metoposcopy and kindred arts.

Metoposcopy and kindred arts.


The exotic animal problem.

The exotic animal problem.


Subcutaneous mastectomy for central tumors of the breast, with immediate reconstruction.


Disseminated cytomegalovirus infection associated with death in a patient with severe facial injuries. Case report.


The ventromedial hypothalamus and aggressive behaviors.


Nutrition, metabolism, and blood lipids in humans with type II A  hyperlipoproteinemia.


A comparison of the motivational properties of thirst induced by intracranial angiotensin and by water deprivation.


Depression of the H-reflex during tooth grinding in sleep.


The role of pancreatic enzymes in digestion.


Calcium metabolism in pancreatic disease.


Papio cynocephalus age determination.

Papio cynocephalus age determination.


Modulation of visual cortex inhibition during reticular evoked arousal.


Noradrenergic control of circadian rhythms in Cebus albifrons.


Tumors in nonhuman primates: observations during a six-year period in the Yerkes primate center colony.


Lead poisoning in zoo-dwelling primates.


Herpesvirus simiae infection in Macaca radiata.


Infectious diseases in primates--introduction and bibliography.


Infectious diseases of primates related to capture and transportation.


The role of H 2 O 2  generation in perfused rat liver and the reaction of catalase compound I and hydrogen donors.


The essential role of the imidazole group of glucagon in its biological function.


The reaction between indole 3-acetic acid and horseradish peroxidase.


Pyridine nucleotide biosynthesis in Claviceps.


Cytochrome c interaction with membranes. I. Use of a fluorescent chromophore in the study of cytochrome c interaction with artificial and mitochondrial membranes.


Isolated adrenal cortex cells: ACTH agonists, partial agonists, antagonists; cyclic AMP and corticosterone production.


Adenylate cyclase from guinea pig lung: further characterization and inhibitory effects of substrate analogs and cyclic nucleotides.


Regulation of glycogen metabolism in the adrenal gland. IV. The effect of insulin on glycogen synthetase, phosphorylase, and related metabolites.


Stimulation of the (Na +  + K + )-dependent adenosine triphosphatase by amino acids and phosphatidylserine: chelation of trace metal inhibitors.


Some regulatory properties of glycogen phosphorylase from Streptococcus salivarius.


Role of mitochondria in the sex-directed flocculation of a fission yeast.


The effects of adenine nucleotides on NADH binding to mitochondrial malate dehydrogenase.


Proteins of the thermophilic fungus Humicola lanuginosa. II. Some physicochemical properties of a cytochrome c.


The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.


Initiation of protein synthesis in vivo in poliovirus-infected HeLa cells.


Substrate inhibition of soluble and bound lactate dehydrogenase (isoenzyme 5).


Phospholipase C purification and specificity with respect to individual phospholipids and brain microsomal membrane phospholipids.


Effects of nucleotides and Na +  on ouabain activation of the phosphatase associated with Na + , K + -ATPase.


Ro le of phospholipids in the NA + ,K + -stimulated adenosine triphosphatase system of brain microsomes.


Ultraviolet sensitivity of helper function of murine leukemia virus.


Isolation of a herpesvirus from a pigeon.


The nature and treatment of skin conditions observed in horses in Queensland.


Redox interactions between cytochrome components and transition ions and metals.


Zonal diffusion, a new technique. Application to the determination of the molecular weight of M13 viral deoxyribonucleic acid.


Electron paramagnetic resonance studies on cobalamin-dependent ribonucleotide reduction.


Separation of phospholipids from glucose-6-phosphatase by gel chromatography. Specificity of phospholipid reactivation.


Kinetics and equilibria in partially reduced flavine solutions.


Guanine phosphoribosyltransferase from Escherichia coli, specificity and properties.


States of hemoglobin in solution.

States of hemoglobin in solution.


Occurrence and properties of a chromatin-associated F1-histone phosphokinase in mitotic Chinese hamster cells.


Direct incorporation of fatty acids into the halosulfatides of Ochromonas danica.


Isolation and fractionation of yeast nucleic acids. I. Characterization of poly(L-lysine) kieselguhr chromatography using yeast nucleic acids.


Isolation and fractionation of yeast nucleic acids. II. Rapid isolation of mitochondrial deoxyribonucleic acid by poly(L-lysine) kieselguhr chromatography.


Effects of catecholamines and adenosine derivatives given into the brain of fowls. 1. Adult fowls (Gallus domesticus) with cannulae chronically implanted into the IIIrd cerebral ventricle and various other sites of the brain received microinfusions or injections of catecholamines, adenosine, 3',5'-cyclic AMP or its dibutyryl derivative. The effects of these substances on behaviour, electrocortical activity and body temperature were studied.2. Behavioural and electrocortical sleep with fall in body temperature were obtained with intraventricular noradrenaline, alpha-methylnoradrenaline and isoprenaline; dopamine was ineffective. The doses required to elicit sleep were smaller than those affecting body temperature. Following mebanazine, the effects of noradrenaline were prolonged and doses of dopamine, previously ineffective, lowered body temperature and induced behavioural and electrocortical sleep.3. Noradrenaline, alpha-methylnoradrenaline, isoprenaline and dopamine infused into the hypothalamus induced sleep and lowered body temperature. Effective doses of noradrenaline, alpha-methylnoradrenaline and isoprenaline infused into the hypothalamus were one-twentieth to one-fifth those for intraventricular injection. Tachypnoea developed with isoprenaline and dopamine. Additionally with dopamine, there was deviation of the head to the contralateral side, together with repetitive jerking movements of the head. These effects were prolonged and intensified by mebanazine, whereas the involuntary movements with dopamine were greatly reduced by haloperidol.4. Involuntary movements, but without sleep, were induced by infusing dopamine into the paleostriatum augmentatum; noradrenaline infused into this site was ineffective.5. In three of five fowls pretreated with aminophylline, 3',5'-cyclic AMP infused into the hypothalamus induced behavioural and electrocortical sleep; without aminophylline pretreatment, 3',5'-cyclic AMP was ineffective. Adenosine infused into the hypothalamus, following pretreatment of fowls with aminophylline, consistently induced behavioural and electrocortical sleep. Dibutyryl cyclic AMP infused into the hypothalamus of intact fowls elicited behavioural arousal, followed by bursts of electrocortical spikes (6 Hz) over both cerebral hemispheres, spikes subsequently becoming regular at 1 Hz. Clonic limb and body movements occasionally accompanied the bursts of spike activity, infrequently developing into convulsions. In fowl encéphale isolé preparations, in which dibutyryl cyclic AMP was infused into the hypothalamus, spike activity was confined to the ipsilateral hemisphere.


Effect of fast-growing transplantable hepatoma on cell proliferation in host tissue of the mouse.