Cytotoxic effects of streptozotocin and N-nitrosomethylurea on the pancreatic B cells with special regard to the role of nicotinamide-adenine dinucleotide. The effects on the pancreatic B cell of streptozotocin and its aglucone derivative N-nitrosomethylurea were investigated in obese-hyperglycaemic mice and their lean littermates. Both streptozotocin and N-nitrosomethylurea were found to be B-cytotoxic although N-nitrosomethylurea produced less islet damage. Both substances decreased the concentrations of NAD(+) in the islet cells to about 10% of the control values within 2h after injection. This NAD(+) depletion was prevented by injection of nicotinamide 10min after the administration of streptozotocin or N-nitrosomethylurea. In islets taken from animals 10min after injection of streptozotocin or N-nitrosomethylurea there was no stimulatory effect of glucose on the respiration or insulin release and the oxidation of glucose was markedly decreased. Addition of nicotinamide (10mm) to the incubated islets restored glucose stimulation of both the oxygen consumption and insulin release. It is concluded that islet NAD(+) depletion is probably important for the B-cytotoxin action of N-nitrosomethylurea and streptozotocin. The glucose residue in the streptozotocin molecule may potentiate the B-cytotoxic action of this drug in mice.


Involvement of binding lipoproteins in the absorption and transport of alpha-tocopherol in the rat. 1. Specific lipoproteins binding alpha-tocopherol but not its known metabolites have been isolated and identified from cytosol of rat intestinal mucosa and from serum. 2. A timestudy of the appearance of the orally administered alpha-[(3)H]tocopherol with these lipoproteins indicates that very-low-density lipoprotein of serum acts as a carrier of the vitamin. 3. The involvement of the mucosal lipoprotein in the absorption of the vitamin from the intestine has been inferred from observations on the amounts of alpha-tocopherol in serum of orotic acid-fed rats where release of lipoproteins from the liver to serum is completely inhibited. A considerable decrease in the association of alpha-tocopherol with serum very-low-density lipoprotein under this condition is interpreted to mean that serum lipoproteins are limiting factors for the transport of the vitamin across the intestine and that this is possibly effected by exchange of alpha-tocopherol between serum very-low-density lipoprotein and mucosal lipoprotein.


Effects of alpha-amanitin on the stimulation of prostatic ribonucleic acid polymerase by prostatic steroid-protein receptor complexes. Stimulation of prostatic RNA polymerase in vitro by prostatic 17beta-hydroxy-5alpha-androstan-3-one (5alpha-dihydrotestosterone)-receptor complexes has been previously reported. By use of the selective inhibitor, alpha-amanitin, we have shown that both nucleolar and extranucleolar RNA polymerase activities may be stimulated, but stimulation is abolished at high ionic strength.


Cell-free biosynthesis of polyprenyl derivatives of p-hydroxybenzoic acid in mammalian and bacterial systems.


The metabolism of phosphatidylcholine in brain tissue.


Inositol lipid metabolism and the cell membrane.


The role of lipids in membrane structure.


Biosynthesis of unsaturated fatty acids by higher plant systems.


Cellular control of the uptake, storage and release of noradrenaline in sympathetic nerves.


The influence of nerve-impulse flow on the synthesis and metabolism of 5-hydroxytryptamine in the central nervous system.


The storage and release of acetylcholine by cholinergic nerve terminals: recent results with non-mammalian preparations.


Adenosine 3':5'-cyclic monophosphate as a mediator in the action of neurohumoral agents.


Effect of allopurinol on the synthesis of corticosterone in rat adrenals in vitro.


Inhibition of various cyclic AMP phosphodiesterases by pentifylline and theophylline.


Wet fallout of sulfurous pollutants emitted from a city during rain or snow.


An unusual mandibular first molar. A case report.


A tumour cell aggregation promoting substance from rat ascites hepatoma cells. A substance capable of promoting tumour cell aggregation was released from rat ascites hepatoma cell (possibly from the cell surface) kept in Hanks' balanced salt solution (free of calcium and magnesium) in the cold, and then partially purified by chromatography with DEAE-Sephadex and gel filtration with Bio-gel. The thermostable substance seemed to be a glycoprotein and its molecular weight was about 72,000 when measured by gel filtration on Sephadex G-200. It had no proteolytic activity. The material was clearly effective for rat ascites hepatoma cells as well as SV40 transformed cells, but less effective for Chang's cells and apparently ineffective for normal rat liver cells and red blood cells. The action of this material was more potent than that of Jack bean concanavalin A when assayed for aggregation of SV40 transformed cells. Its effect was not influenced by concanavalin A inhibitors such as alpha-methyl-D-glucopyranoside, N-acetyl-D-glucosamine and D-glucose.


Ectopic production of human chorionic gonadotrophin by human breast tumours. The incidence of tumours ectopically producing the human chorionic gonadotrophins was studied in patients with breast cancer. Specific radioimmunoassay of subunits of HCG was utilized. Nine out of 65 patients with carcinoma of breast showed the presence of circulating HCG. Patients with other pathological conditions of breast tissue did not show any evidence of circulating HCG.


Dephosphorylation of rat liver ribosomal proteins by a phosphoprotein phosphatase.


Beef pancreas tryptophanyl-tRNA synthetase. Order of substrate binding in ATP-(32-P)-pyrophosphate exchange reaction.


Intramitochondrial intermembranal large amplitude protein movements. I. A possible novel aspect of membrane fluidity.


Research of specific constraints in pair association of amino acids.


Release of prostaglandins from the rabbit perfused kidney: effects of vasoconstrictors. 1 The rat stomach strip was used to assay prostaglandin E(2)-like material released by a rabbit isolated kidney perfused with Krebs solution.2 Doses of noradrenaline and angiotensin II producing similar vasoconstrictor effects released equivalent amounts of prostaglandins from the kidney.3 8-Leu-angiotensin II, a specific inhibitor of the natural octapeptide, blocked the action of angiotensin II on perfusion pressure and the release of prostaglandins, while the action of noradrenaline on both parameters was unaffected.4 Indomethacin, a specific inhibitor of prostaglandin biosynthesis, blocked the effects of both vasoconstrictors on prostaglandin release while their action on perfusion pressure was significantly enhanced.5 In the kidney effluent, amounts of prostaglandin E(2)-like material increased linearly with the rise in perfusion pressure induced by increasing doses of angiotensin II. These results indicate that prostaglandin output from the isolated kidney follows the rise in perfusion pressure.


Dissociation of 5-hydroxytryptamine effects on myocardial contractility and cyclic AMP accumulation. 1 5-Hydroxytryptamine increased the contractility of the isolated rat atrium and produced a small rise in cyclic 3',5'-adenosine monophosphate (cyclic AMP) accumulation in rat heart slices. These effects were similar to those of tyramine.2 Incubation with phenoxybenzamine prevented the inotropic effect of 5-hydroxytryptamine but did not inhibit the rise in cyclic AMP accumulation.3 A single injection of reserpine 24 h before the experiment did not inhibit the inotropic effect of 5-hydroxytryptamine but prevented the rise in cyclic AMP accumulation.4 It is concluded that in the rat 5-hydroxytryptamine increases cardiac contractility chiefly by a direct effect which is blocked by phenoxybenzamine, whereas it increases myocardial cyclic AMP accumulation indirectly by releasing endogenous catecholamines. Cardiac contractility may thus be increased without a rise in cyclic AMP accumulation, and cyclic AMP accumulation may be increased without a rise in contractility.


The microbiology and sensory evaluation of pheasants hung at 5, 10 and 15 degrees C.


Sexually-transmitted diseases in animals.


The effect of phenytoin on parathyroid extract and 25-hydroxycholecalciferol-induced bone resorption: adenosine 3, 5 cyclic monophosphate production.


Studies on dentinogenesis in the rat. The interaction between lead-pyrophosphate solutions and dentinal globules.


The conversion of cytochrome oxidase to an inactive form of the enzyme by water dialysis.


Histochemical studies on alkaline phosphatases in mineralizing oral tissues in the mouse.


Phosphoribosyl pyrophosphate degradation in human tissues.


The goldfish pituitary. I. Cytology.

The goldfish pituitary. I. Cytology.


Cell autolysis and deletion in the planarian Polycelis tenuis Iijima.


Fine structure and function of the alimentary epithelium in Artemia salina nauplii.


The ACTH-interrenal axis in the freshwater stickleback, Gasterosteus aculeatus form leiurus.


Ultracytochemistry of the lung of Polypterus ornatipinnis.


Coronet cells in the neurohypophysis of Mugil capito Cuvier and Valenciennes (Teleostei) from a hypersaline lagoon.


Ultrastructural study of arterio-venous anastomoses in gill filaments of Tilapia mossambica.


Electron microscopic autoradiography of Ca-45 during dentinogenesis.


The hypothalamo-hypophysial system of hypophysectomized rats. I. Ultrastructure of nerve fibres in "intact" and dehydrated animals.


Comparison of postoperative irradiation alone and in combination with BCNU (NSC-409962) in the management of malignant gliomas.


Phosphoriboxyl transferase in colon tumor and normal mucosa as an aid in adjuvant chemotherapy with 5-fluorouracil (NSC-19893).


Inhibition of Rauscher leukemia virus and avian myeloblastosis virus DNA polymerases by tilorone (NSC-143969) and its analogs.


The metabolism of low-density lipoprotein in a patient with familial hyperbetalipoproteinaemia.


Changes in plasma lipoprotein lipids in hypercholesterolaemic patients treated with the bile acid-sequestering resin, colestipol.


A new approach to estrogen-free contraception based on progesterone receptor blockage by mid-cycle administration of ethyl norgestrienone (R 2323).


Microbiological assay in quantitative histochemistry.


SQ 65,396: a non-sedative anxiolytic?

SQ 65,396: a non-sedative anxiolytic?


Recent progress in studies of mouse type-C viruses.


The biochemistry of icosahedral cytoplasmic deoxyviruses.


Mode of existence of corticosterone in adrenal cells with reference to storage and secretion.


Phosphorylation of nuclear proteins fron bovine anterior pituitary gland induced by adenosine 3':5'-monophosphate.


Effect of ACTH and dexamethasone on the rate of protein and RNA degradation in rat adrenal glands.


The role of lysine and leucine binding on the catalytical and structural properties of aspartokinase III of Escherichia coli K 12.


The mechanism of inhibition of pig-plasma benzylamine oxidase by the copper-chelating reagent cuprizone.


Effect of azide on some spectral and kinetic properties of pig-plasma benzylamine oxidase.


Synthesis of ATP from ADP and inorganic phosphate at the myosin-subfragment 1 active site.


A high-molecular-weight protein component required for natural messenger translation in ascites tumor cells.


Chemical studies on Shigella sonnei lipid A.


Adenylate kinase of porcine heart.

Adenylate kinase of porcine heart.


Effect of imipramine on the behavior of rat-liver mitochondria during centrifugation in a sucrose gradient.


The covalently bound flavin of Chromatium cytochrome c552. 1. Evidence for cysteine thiohemiacetal at the 8 alpha position.


Differences between the estradiol and testosterone receptors in rat uterus.


Synthesis and properties of N6, C-8 and C-2 spin-labelled derivatives of adenosine cyclic 3':5'-monophosphate.


Studies on the metabolic effects induced in the rat by a high-fat diet. Control of glucose metabolism in adipose tissue of fed and fasted rats.


The interaction of apoprotein from porcine high-density lipoprotein with dimyristoyl phosphatidylcholine.


The interaction of apoprotein from porcine high-density lipoprotein with dimyristoyl phosphatidylcholine. Electron spin resonance and fluorescent probe. studies.


Proceedings: Multiple forms of adenosine 3':5'-cyclic monophosphate phosphodiesterase in bovine corpus luteum.


Proceedings: Partial purification and initial observations of molecular weights of luteal adenosine 3':5'-cyclic monophosphate-binding proteins.


Proceedings: Adenosine 3':5'-cyclic monophosphate-binding proteins in rat corpus luteum.


Proceedings: Rat luteal adenosine 3':5'-cyclic monophosphate-dependent protein kinase.


Proceedings: The effect of an ovulatory dose of human chorionic gonadotrophin on preovulatory adenosine 3':5'-cyclic monophosphate concentrations in rabbit ovarian tissue.


Proceedings: Variation in urinary adenosine 3':5'-cyclic monophosphate contend during the human menstrual cycle.


Proceedings: Control of glycerokinase activity by sex hormones.


Protein composition in developing human brain myelin.


Cysteamine-induced duodenal ulcers: a new model to test antiulcer agents.


Congenitally impaired hormone sensitivity of the adipose tissue of spontaneously diabetic mice, KK.  Validity of thrifty genotype in KK mice.


The lipolytic system in adipose tissue of Toronto-KK and C57BL/KsJ diabetic mice. Adenylate cyclase, phosphodiesterase and protein kinase activities.


Diabetes in the Toronto-KK hybrid mouse abnormalities in liver and pancreatic islets of Langerhans.


Lipolysis and adenosine 3'5'-cyclic monophosphate in adipose tissue of the New Zealand obese mouse; the activities of adipose tissue adenyl cyclase and phosphodiesterase.


Control of insulin secretion in the Egyptian sand rat ((Psammomys obesus).


Evidence for decreased sensitivity to glucose of isolated islets from spiny mice. (Acomys cahirinus).


Physiological studies of salmonella histidine operator-promoter mutants. S. typhimurium hisO mutations are cis dominant and trans recessive and occur in a regulatory segment separate from but adjacent to the first structural gene of the histidine operon, hisG. Strains containing hisO mutations singly and in combination with other regulatory mutations were examined for their content of L-histidinol phosphate phosphatase when grown on limiting and on excess L-histidine. HisO mutations classed as "constitutive" (high enzyme levels) or as "promoter-like" (low enzyme levels) cause a variety of sub-phenotypes. A model is proposed that accounts for the phenotypes found as well as for the interspersion of constitutive and promoter-like mutations on the genetic map (Ely, Fankhauser and Hartman 1974). In this model we suggest that the his operator-promoter DNA is a functional unit that assumes alternate conformations including: (a) the classic linear duplex, active in transcription, and (b) a looped structure that is transcriptionally closed and susceptible to the binding of repressor.


The clinical significance of immunological findings in Burkitt's lymphoma. (Epstein-Barr virus-associated antibodies/BCG treatment.


Tumor virus RNAs tumor virus genes.

Tumor virus RNAs tumor virus genes.


Electron microscopic and cytochemic findings in Di-Guglielmo's syndrome and in other forms of leukemia.


DNA polymerases in lymphoid cells.

DNA polymerases in lymphoid cells.


Histochemical studies on the liver of white rats at various intervals after transection of the vagus nerves.


Histochemical studies on the gastric epithelium and glands in guinea pigs subjected to the action of a fungicide (Kaptan).


Abnormal duodenal loop in patients with sliding esophageal hiatal hernia -- studies on intragastric pressure and gastrocolic reflex.


Infrared spectra of DNA from normal & urethan-treated mice.


Enzyme electrophoresis on cellulose acetate gel. II. Zymogram patterns in man-Chinese hamster somatic cell hybrids.


Effect of exogenous 3',5'-cyclic adenosine monophosphate on the cell surface properties of the cellular slime mould, Dictyostelium discoideum.


Purification and properties of a new extracellular collagenase from Aspergillus sclerotiorum.


Permeation of nucleotidic coenzymes into liver cells.


The purification and new properties of the neutral proteinase in human semen.


Endometrial biopsy using Gravlee Jet Washer and suction curette.


Effects of microquantities of 5 alpha-dihydrotestosterone on the epididymis and accessory glands of the castrated rhesus monkey, Macaca mulatta.


Effect of ageing on the volume, structure and total Leydig cell content of the human testis.


Sex ratio in extrauterine pregnancies.

Sex ratio in extrauterine pregnancies.


Scanning electron microscopy of boar spermatozoa before and after frozen-thawed treatment.


The influence of adoption on subsequent pregnancy in infertile marriage.


The possible role of kinins in sperm motility.


Post tubal ligation menorrhagia and pelvic pain.


Clinical evaluation of a new low dose progestagen-only contraceptive containing cingestol.


Survival of Entamoeba and related amoebae at low temperature-II. Viability of amoebae and cysts stored in liquid nitrogen.


Observation of surface active lysosomes and other organelles in monoxenically cultivated trophozoites of ent amoeba histolytica and Ent amoeba coli.


Isolation of avian nephroblastoma virus from avian myeloblastosis virus by the infectious DNA technique.


Analysis of chromosomes of syrian hamster cells transformed with human cytomegalovirus.


A systematic approach to sleep disorders: review and treatment.


Different patterns of chromosome exchange induced by methyl-metanesulphonate and mitomycin C in human cells.


Minor salivary glands of man: enzyme and mucosubstance histochemical studies.


Salivary glands of the cat: a histochemical study.


Adenylate cyclase in an estradiol sensitive tissue: a cytochemical study.


Fine structure of an edentate (Dasypus novemcinctus) male reproductive system: the epididymis.


Proceedings: Small dense-cored vesicles in the cholinergic nerve terminals of the rat bladder.


Proceedings: Human mast cell degranulation and vacuolation.


Proceedings: Early Tasmanian morphology - new archaeological evidence.


Trigeminal motor neuropathy.

Trigeminal motor neuropathy.


Neurological episodes following epidemic conjunctivitis.


Inhibition of growth and DNA synthesis in cell cultures by cyclic AMP.


Nucleolar development in the interphase of the cell cycle.


Descriptive and quantitative study of ultrastructural changes in the apical meristem of mustard in transition to flowering. II. The cytoplasm, mitochondria and proplastids.


Cytokinesis in the heliozoan Actinophrys sol.


The chloroplast nucleoid in Ochromonas danica. I. Three-dimensional morphology in light- and dark-grown cells.


The chloroplast nucleoid in Ochromonas danica. II. Evidence for an increase in plastid DNA during greening.


Avian sarcoma virus transformed hamster cells made resistant to ethidium bromide.


The subcellular distribution of prolyl hydroxylase determined immunohistochemically.


Cytotoxic compounds. Part XX. Reactions of the methanesulphonates of 2-aryl-2-(arylthio)ethanols and of 1-aryl-2-(arylthio)ethanols with nucleophiles.


Relation of enterotoxigenic Clostridium perfringens type A to food poisoning. I. Effect of heat activation on the germination, sporulation and enterotoxigenesis of c. perfringens.


Mutants of Aspergillus nidulans impaired in penicillin biosynthesis.


The effect of Rowson-Parr virus on the severity of malaria in mice.


Segmental reflex pathways in spinal shock and spinal spasticity in man. Activity in three segmental pathways was compared in normal subjects, patients with spinal shock, and patients with established spinal spasticity. The Achilles tendon reflex (ATR) was used to estimate transmission in the Ia monosynaptic pathway. Evidence is produced implying that vibration activates motoneurones principally through a polysynaptic pathway. The tonic vibration reflex (TVR) was used to estimate transmission in this Ia polysynaptic pathway. The percentage of the motoneurone pool (M-response) that could be activated by these pathways was used as a measure of transmission. The H reflex (vibration)/H reflex (control) ratio was used as an estimate of the degree of presynaptic inhibition of the Ia monosynaptic pathway. The findings led to the following conclusions. (1) In spinal shock presynaptic inhibition is greater than normal, transmission in the Ia monosynaptic pathway is reduced, and in the Ia polysynaptic pathway virtually abolished. (2) In established spasticity presynaptic inhibition is impaired, transmission in the Ia monosynaptic pathway is increased, but transmission in the Ia polysynaptic pathway never recovers. (3) The failure of presynaptic inhibition associated with spasticity is a gradual process. A hypothesis to explain these findings is proposed.


Regional cerebral blood flow and intraventricular pressure in acute head injuries. Twelve patients who were comatose after head injuries were studied with serial determinations of regional cerebral blood flow, jugular PO(2) tension, and intraventricular pressure. These determinations began a few hours after the injury, and were followed throughout the clinical course. Diffuse derangement of cerebral vasomotor regulation is confirmed after severe head trauma, which may contribute to deterioration and poor prognosis, and which indicates a need for therapeutic maintenance of rich oxygenation, hyperventilation with moderate hypocapnia, and steady blood pressure. Continuous recording of IVP (eventually sensitized by fluid infusion or CO(2) inhalation tests) may give an early indication of the subsequent clinical state and may suggest the need to submit the patients to further investigative and therapeutic procedures.


Ischaemic lateral popliteal nerve palsy due to ergot intoxication. A patient with ergot intoxication due to medication for migraine developed lateral popliteal nerve palsy. This is attributed to recurrent ischaemia caused by ergotamine.


A revised boron-neutron capture therapy for malignant brain tumours. I. Experience on terminally ill patients after Co-60 radiotherapy.


Amyloid neuropathy: report of a case verified by autopsy.


Anticonvulsant activity and selective inhibition of nicotinamide adenine dinucleotide-dependent oxidations by 1,3,5-trisubstituted pyrazolines.


Verapamil blockade of slow Na+ and Ca++ responses in myocardial cells.


Studies of fetal mouse hearts in organ culture: influence of prolonged exposure to triiodothyronine on cardiac responsiveness to isoproterenol, glucagon, theophylline, acetylcholine and dibutyryl cyclic 3',5'-adenosine monophosphate.


Comparative effects of tetracyclines on water flow across toad urinary bladders.


Structure-activity relations for caffeine: a comparative study of the inotropic effects of the methylxanthines, imidazoles and related compounds on the frog's heart. 1. The ability of several groups of compounds, related to caffeine, to induce contractures in isolated frog auricular trabeculae has been tested.2. Of the methylxanthines, theophylline, theobromine and paraxanthine are of similar potency to caffeine. This applies to contractures produced in either high-potassium or in sodium-free solution, and to the twitch responses in normal Ringer.3. Xanthines in which the 9-position nitrogen is combined and is, therefore, without an ethylene bond do not affect contraction.4. The hypothesis is put forward that a double-bonded nitrogen, in an imidazole ring, is required for activity of the methylxanthine. This hypothesis is supported by the ability of imidazole and several close derivatives (e.g. histamine), as well as imidazolines, to evoke contractures. As predicted by the hypothesis, imidazolidines and imidazolidones, in which all the nitrogen atoms have single bonds, fail to initiate tension development.5. The activity of histamine and histidine is only demonstrable at high pH ( bumpy equals, equals 9.0).6. Raising the pH in sodium-free solution induces a transient contracture.7. Several arguments suggest that cyclic AMP is probably not an intermediate in the response to the methylxanthines. The activity of cyclic AMP (and adenosine) in eliciting contractures is predicted by the hypothesis because they contain an imidazole moiety as part of their molecular structure.


The rate and depth of breathing in new-born infants in different sleep states. 1. Ventilation was recorded on ten male and ten female healthy full-term infants during the first week after delivery, using a trunk plethysmograph. Tidal volume (V(T)), respiration rate (f) and pulmonary ventilation (V) for each respiratory cycle were measured during periods of rapid eye movement sleep (REM) and during quiet sleep when eye movements were absent (NREM).2. It was found that mean instantaneous V and f were significantly higher in all infants during REM than during NREM sleep, while mean V(T) was either unchanged or showed a decrease. In addition, there was significantly greater variation in instantaneous V, V(T) and f during REM as compared with NREM sleep.3. Positive correlations were found in most infants in both sleep states between individual values of V(T) and the duration of the respiratory cycle (T).4. Periodic changes in T were found in all infants during both sleep states; these periodicities may reflect the behaviour of respiratory control mechanisms operating over a longer time span than the individual respiratory cycle.


Effect of ouabain on renin secretion in anaesthetized dogs. 1. Pentobarbitone anaesthetized dogs were used to study the effects on renin secretion of ureteral occlusion and partial aortic clamping before and after intrarenal arterial administration of ouabain.2. Arterial plasma renin activity (A renin) and renal venous minus arterial plasma renin activity (RV-A renin) were both increased by either ureteral occlusion during mannitol diuresis or partial aortic clamping to reduce renal perfusion pressure. Intrarenal arterial ouabain administration blunted or abolished the effects of these manoeuvres on A and RV-A renins.3. The observations are not consistent with the hypothesis that renin secretion is uniquely controlled by afferent arteriolar transmural pressure. Both ureteral occlusion and aortic clamping reduce this pressure and increase renin secretion, yet evidence presented herein suggests that ouabain, which also decreases this pressure, decreases renin secretion.4. The observations are consistent with the hypothesis that renin secretion is controlled by tubular fluid flow and/or composition in the region of macula densa cells. Possibly changes in flow and/or composition are detected as changes in macula densa intracellular sodium concentration.


The distribution, activity, and function of the cilia in the frog brain. 1. The distribution, activity, and function of the cilia in the brain was studied using in vitro preparations of the frog choroid plexuses and ependyma.2. Scanning electron microscopy revealed that twenty to forty cilia, about 20 mum long, project from the cells of the choroidal epithelium and ependyma into the ventricular system of the brain.3. These cilia beat at a constant frequency which ranged from 5 to 20 c/s. Ciliary activity was enhanced by ATP, cyclic AMP, theophylline, and acetylcholine, and was depressed by DNP, IAA, Ni(2+), La(3+), and Co(2+).4. Ciliary motion produced a flow of c.s.f. over the surface of the cells lining the ventricles, and in the choroid plexus this flow reduced the effective thickness of the unstirred layer adjacent to the epithelium by about 100 mum.5. These results are discussed in relation to the factors that control the frequency of ciliary beating, and the role of the cilia in the circulation of the c.s.f.


Factors influencing plasma renin and angiotensin II in the conscious pregnant ewe and its foetus. 1. Plasma renin (measured in the presence of additional substrate) was significantly higher (10.7 +/- 1.1 S.E. of mean ng/ml.hr) in foetal lambs of 111-144 days gestation age (full term 147 days) than in their mothers (1.5 +/- 0.2 ng/ml.hr S.E. of mean, P < 0.001) but plasma angiotensin II concentrations were in the same range (ewe 47.3 +/- 6.6 S.E. of mean, foetus 47.4 +/- 14.1 S.E. of mean pg/ml.). The endogenous velocity of renin production by foetal plasma was also greater than that of maternal plasma.2. Foetal plasma [Na(+)] (137 +/- 0.8 S.E. of mean m-equiv/l.), was lower than that in the ewe (142 +/- 1.5 m-equiv/l. S.E. of mean, P < 0.01).3. Foetal plasma renin in lambs of less than 120 days gestation was lower (9.2 +/- 2.7 S.E. of mean ng/ml.hr) than that in lambs of over 130 days gestation (12.6 +/- 2.6 ng/ml.hr S.E. of mean, P < 0.01). Foetal plasma [K(+)] (3.8 +/- 0.1 S.E. of mean m-equiv/l.) was also lower in lambs of less than 120 days gestation than in those over 130 days (4.1 +/- 0.1 S.E. of mean m-equiv/l., P < 0.001).4. When small volumes of blood (</= 3% of blood volume) were withdrawn from foetal lambs, plasma renin increased. The% increase of plasma renin in hypoxaemic foetal lambs was significantly less (P < 0.05) than in control lambs. At the end of 60 min hypoxaemia, arterial pressure and plasma [K(+)] were significantly higher in hypoxaemic than in control foetal lambs.5. During foetal hypoxaemia, plasma angiotensin II concentration increased concurrently with plasma renin.6. Bilateral nephrectomy was performed in two foetal lambs. Plasma renin fell to very low levels and angiotensin II became undetectable.7. Adrenaline ( approximately .0.42 mug/min.kg I.V.) infused into the foetus did not alter foetal plasma renin. When adrenaline was infused into the ewe ( approximately 0.26 mug/min.kg) maternal plasma renin increased. Maternal infusion of adrenaline raised foetal plasma renin significantly more (P < 0.05) than foetal infusion.8. It is concluded that the foetal kidney is the major source of foetal renin in the last quarter of gestation and that renin release is stimulated by very small reductions of blood volume. Hypoxaemia does not augment renin release and cannot be responsible for high levels of renin and angiotensin associated with vaginal delivery.


[Sleep-wakefulness cycle in adult rats applied arsenic trioxide from birth to weaning (author's transl)].


The advantages of HeLa cells for isolation of rhinoviruses.


Reinvestigations of the relationship between sporulation, heat resistance and some biochemical properties in strains of Clostridium perfringens.


Variation in polymyxin sensitivity among colonies in primary plate cultures of Vibrio eltor.


Prevalence in Italy of antibodies to a new human papovavirus (BK virus).


Herpesvirus (strain 0430) lesions in Cercopithecus aethiops.


A procedure for the isolation of Herpesvirus saimiri lymphocyte-associated viruses and blood parasites from monkeys living in the wild.


The half-of-the-sites reactivity of horse liver alcohol dehydrogenase in the presence of alcohol substrates.


Action of Escherichia coli P1 restriction endonuclease on simian virus 40 DNA.


Half-of-the-sites and all-of-the-sites reactivity in rabbit muscle glyceraldehyde 3-phosphate dehydrogenase.


Nucleotide alterations in the bacteriophage T4 glutamine transfer RNA that affect ochre suppressor activity.


Letter to the editor: In vivo incorporation of cytosine arabinoside into simian virus 40 DNA.


Assessment of steatorrhoea in malnourished children.


Adrenergic receptors in rat liver. II. Effects of phenylephrine, isoproterenol and adrenergic blocking agents on gluconeogenesis in perfused liver.


Effects of cytochrome C on lipid and sugar metabolism in aged rats.


Histopathological studies of the effects of cytochrome C on carbon tetrachloride-induced liver damage and on the livers of aged rats.


Failure to induce neoplasma in CBA mice inoculated with highly attenuated Herpes simplex virus (SKa strain).


Migration inhibition test in mice inoculated with Herpes simplex virus.


Canine respiratory virus infections.

Canine respiratory virus infections.


Functional islet cell tumour in the dog.


Fluorescence and circular dichroism studies on human serum low density lipoprotein particles and lipid-depleted derivatives.


Cyclic AMP: purification of protamine kinase.


Affinity chromatography of vertebrate collagenases.


Affinity chromatography of urokinase.

Affinity chromatography of urokinase.


Affinity chromatography of steroid-transforming enzymes.


Separation of isozymes.

Separation of isozymes.


Lactate dehydrogenase: specific ligand approach.


Affinity chromatography of phosphodiesterase from snake venom.


Affinity chromatography of insulin receptors.


Affinity chromatography of estrogen receptors.


Topics in the methodology of substitution reactions with agarose.


Affinity chromatography of thyrotropin receptors and antibody.


Influence and control; insurance for nursing's future.


Nursing practice in a changing world.

Nursing practice in a changing world.


External degree potential for Minnesota.


Neonatal chemical sympathectomy: functional control of denervation of the vascular system and tissue noradrenaline level in the rat after 6-hydroxydopamine.


Rat diaphragm cyclic nucleotide-phosphodiesterase: influence of drugs affecting skeletal muscle contractility.


Pituitary adrenal function after small and large lesions in the lateral septal area in food-deprived rats.


Effect of nomifensine on motor activity, dopamine turnover rate and cyclic 3',5;-adenosine monophosphate concentrations of rat striatum.


Demonstration of nuclear 3H-estradiol binding in hypothalamus and amygdala of female, androgenized-female, and male rats. The subcellular distribution of radioactivity in female, androgenized female, and male rats 1 hour after the iv administration of tritiated estradiol was examined.  High regional specificity among nucle ar fractions of brain areas was seen with the preoptic-anterior hypothal amic area (POA-AH), median eminence-basal hypothalamus (ME-BH), and amygdala having much higher nuclear binding than other brain  areas.  Nuc lear binding was receptor-mediated in POA-AH, ME-BH, amygdala, dorsal hypothalamus, and the prehypothalamic area in all groups, as demonstrate d by competetive inhibition with diethylstilbestrol (DES).  At this time period no differences between male and female rats in DES-blockable radioactivity levels of either nuclear or supernatant fractions in any brain area or pituitary were observed.  Androgenized females had lower DES-blockable, nuclear radioactivity levels in POA-AH, ME-BH, and uteri but the % of total tissue radioactivity in the nuclei did not differ between groups for any tissue.  These results demonstrate that males and females have similar estradiol uptake systems that are capable of high levels of nuclear binding in various brain areas.


Inhibition of fluid transport in the isolated gall bladder of the guinea pig by isoprenaline, theophylline and cyclic adenosine 3',5'-monophosphate.


Androgen effects on follicle-stimulating hormone (FSH) secretion in organ culture.