A mutant of 3T3 cells with cyclic AMP metabolism sensitive to temperature change. A mutant line of 3T3 (mouse fibroblast) cells with temperature-sensitive cyclic AMP (cAMP) metabolism was isolated by selecting for low substratum adhesiveness after a change in temperature. Although the mutant is identical in behavior to the parent cell at constant temperature, a rise or fall in temperature causes a fall in intracellular cAMP levels within seconds, followed by loss of adherence to the culture dish and retraction of cell processes. The mechanism of this fall in cAMP level is at least in part excretion into the medium. The decrease in adhesiveness and retraction of processes can be blocked by analogues of cAMP or agents that elevate intracellular cAMP. The properties of this mutant imply that cAMP is a direct regulator of cell shape and adhesiveness.


Isolation of Escherichia coli RNA polymerase binding sites on T5 and T7 DNA: further evidence for sigma-dependent recognition of A-T-rich DNA sequences. Previous isolation and analysis of E. coli RNA polymerase (EC 2.7.7.6) binding sites on lambda DNA had demonstrated the existence of a sigma-dependent process of recognition of A-T-rich DNA sequences. We have now extended this finding to T5 and T7 DNA and häve provided evidence for the double-strandedness of the isolated binding sites. The possible equation of these sites to the genetically defined promoters is discussed.


Mechanisms of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement. A low-molecular-weight component of complement, similar to or identical with human C5a, interacts with human polymorphonuclear leukocytes treated with cytochalasin B and provokes extracellular release of lysosomal enzymes from these cells. Enzyme release occurs in the absence of particles and is selective in that it is not accompained by release of cytoplasmic enzymes. Cell viability is not altered. Pharmacologic agents that regulate secretion of other inflammatory mediators influenced complement-dependent enzyme release: cAMP and theophylline, prostaglandin E(1) and colchicine inhibited, whereas cGMP enhanced release of enzymes. Ultra-structural histochemistry of cells exposed to this component of complement revealed degranulation, fusion of lysosomal with plasma membranes, and transient assembly of microtubules associated with the release of endogenous myeloperoxidase. Our findings suggest that these intracellular events are common to two important responses of polymorphonuclear leukocytes in inflammation and tissue injury: (a) release of lysosomal hydrolases and (b) chemotaxis.


Dynamics of metabolism of normal and virus-transformed chick cells in culture. Application of steady-state tracer technique to normal and transformed cells in tissue culture allows quantitation of intracellular pool sizes of many metabolites and determination of rate of carbon flow along diverse paths. Using a unique apparatus to control the environmental conditions, we show that the glucose carbon flow into tricarboxylic acid cycle intermediates and amino acids is unchanged upon transformation. The increased glycogen formation and glycolysis varies with the glucose concentration in the medium, correlates with the faster glucose transport of transformed cells, and cannot be explained by a difference in growth rate alone.


Direct evidence for messenger activity of influenza virion RNA. In a cell-free system of Escherichia coli, RNA from influenza virus particles is translated into a polypeptide antigenically identical with the ribonucleoprotein and several more proteins, some of which correspond in size to viral structural components.


Linear electric field-induced shifts in electron paramagnetic resonance: a new method for study of the ligands of cytochrome P-450. The linear electric field shift in paramagnetic resonance has, for the first time, been observed in frozen solutions. The magnitude of the shift parameters, measured at g(max) of the electron paramagnetic resonance absorption envelope, has been used to characterize lowspin ferric heme mercaptide complexes where the nonmercaptide ligand was varied. The magnitude of the shift provides a measure of the difference between the crystal field contribution of the mercaptide and nonmercaptide ligands. In low-spin ferric cytochrome P-450 of rat liver, the presence of an electric field shift indicates a deviation from inversion symmetry and proves that the axial ligands to the heme are not the same. From the magnitude of the shift and its dependence upon the angle between applied electric and magnetic fields, it is suggested that the non-axial ligand to the heme has a crystal field contribution greater than imidazole and smaller than guanidine.


Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin. Epidermal growth factor (EGF) stimulates both DNA and RNA synthesis in contact-inhibited human fibroblasts. Stimulation of DNA synthesis is observed at concentrations as low as 3 pM, is half-maximal at 70 pM, and is maximal at 300 pM EGF. The action of EGF is similar to that of fetal-calf serum, but is distinguished by the time-course of stimulation and by the ability of serum to stimulate further those cells maximally stimulated by EGF. Cells that synthesize DNA in response to physiological concentrations of EGF (10(-11) to 10(-10) M) are insensitive to physiological concentrations of insulin (10(-11) to 10(-10) M) and respond only minimally to very high concentrations of this hormone (10(-6) M). The biological activity of EGF is paralleled by binding of this peptide to fibroblasts in a specific and saturable manner; the dissociation constant is about 800 pM. The binding of EGF is unaffected by either insulin or cholera toxin. Cholera toxin inhibits the action of both EGF and serum. Suppression of DNA synthesis is observed at 0.02 pM toxin, and is maximal at about 2 pM. Cells treated with cholera toxin at these concentrations appear to be otherwise viable by several criteria. The stimulatory effects of EGF are also inhibited by theophylline and dibutyryl cyclic AMP separately or in combination. These observations indicate that fibroblasts possess receptors for EGF by biological and physicochemical criteria, and suggest that a similar if not identical peptide may be amongst those factors in sera which stimulate cell growth. The possibility is considered that EGF and cholera toxin modulate the ability of a cell to initiate polynucleotide synthesis by way of specific cell-surface interactions which in turn alter the levels of intracellular cyclic AMP.


Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Syngeneic antisera have been produced in mouse strain 129/Sv-CP males against the primitive cells of teratocarcinoma. These sera react specifically with the primitive cells and are negative on various types of differentiated teratoma cells derived from the same original tumor. They are negative on all other mouse cells tested, with the exception of male germ cells and cleavage-stage embryos. Thus, teratoma cells possess cell-surface antigens in common with normal cleavage-stage embryos.


Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells. RNA from noninfectious virions produced by two established clonal lines of sarcoma positive-leukemia negative (S+L-)-transformed 3T3 cells has been characterized. RNA from virions or nucleoids of S+L--(C243) cells consisted of three to four sizes: +/-44 S (6%), 28 S (17%), 18 S (38%), and <18 S (39%). 28S virion RNA contained some virus-specific information demonstrable by RNA.DNA hybridization with a DNA probe derived from the RNA-directed DNA polymerase product of murine sarcoma-leukemia virus, while parallel studies indicated that 28S ribosomal RNA from ribosomal subunits of transformed and nontransformed 3T3 cells did not contain virus-specific information. In contrast to the S+L-(C243) virions, RNA from virions or nucleoids of S+L-(D56) cells consisted of five sizes: 80 S (6%), 68 S (8%), 56 S (5%), 28 S (28%), and <28 S (53%). Thermal dissociation studies suggested that this S+L- genome is comprised of 28S RNA subunits. From these studies we postulate that the 28S viral RNA is most probably the monomeric genome of S+L- virions.


Development of chloroplast substructures with apparent secretory roles in the young tobacco leaf.


The fine structure of iridosphores in the skin of the Atlantic salmon (Salmo salar L.).


Influence of changes in myocardial contractility on the height and slope of the calibrated apex cardiogram.


Angiographic diagnosis and differential diagnosis of islet-cell tumors.


The many faces of islet cell tumors.

The many faces of islet cell tumors.


Unilobar veno-occlusive disease of the liver. Angiographic demonstration of intrahepatic competition simulating hepatoma.


Obstructive jaundice, the radiologist, the surgeon, and the patient: Caldwell Lecture, 1971.


Conformational studies on guanosine nucleotides and polynucleotides. The effect of the base on the glycosyl and backbone conformations.


An improved methodology for preparation of virus-induced double stranded RNA.


Relations between the ventrolateral thalamic nucleus and motor cortex and their possible role in the central organization of motor control.


Parameters of stimulation of RNA synthesis and characterization by hybridization in a molluscan neuron.


Postnatal development of thalamic synaptic events underlying evoked recruiting responses and electrocortical activation.


Specific neural connections for the cerebellar control of vestibulo-ocular reflexes.


Comparison of effects of paradoxical sleep deprivation and immobilization stress on 5-hydroxyindoleacetic acid in cerebrospinal fluid.


Lyophilized combination pools of enterovirus equine antisera: preparation and test procedures for the identification of field strains of 42 enteroviruses. This paper describes the preparation of 8 dried pools (designated A to H) of sera. Each pool is composed of 10 or 11 of 42 individual enterovirus equine sera and contains 500 antibody units of each serum component per 0.1 ml. Procedures for using the antiserum pools are given, and guidance is provided for interpreting the results of serum neutralization tests in identifying field isolates.


Immunological problems in leprosy research. 1. This Memorandum reviews the present status of knowledge of the immunology of leprosy, with particular attention to developments since the publication of a similar review in 1970. The different types of lepromin reaction and their significance in healthy contacts and in patients with tuberculoid and lepromatous leprosy are discussed. The immunological responsiveness of patients with leprosy is also considered, with special attention to in vitro methods for evaluating this response. Part 2 of the Memorandum will cover possible mechanisms of altered immune response in leprosy (including a tentative scheme to explain the possible genesis of the lepromatous lesion); genetic, nutritional, and hormonal factors; the possibility of vaccination; attempts at immunotherapy; and areas in which further research is needed. A detailed protocol for evaluating the effect of transfer factor in leprosy will be included as an annex.


Standard reference materials: standard quartz cuvettes for high-accuracy spectrophotometry.


Plasma retinol assay by elution from silicic acid with cymene (p-lsopropyl toluene).


Evaluation of a rapid latex-agglutination test for detection of alpha1-fetoprotein. Detection of alpha-fetoprotein in adult sera has proved helpful in diagnosis of primary liver carcinoma;  detection of trace amounts in maternal sera during pregnancy can signal fetal distress and/or fetal death.  A latex-agglutination assay for alpha-fetoprotein (AFP) has been evaluated and compared to results from tests using counter-immunoelectrophoresis (CIEP).  In a series of 50 hepatoma patients, there was 88.6% agreement between the latex-agglutination assay method and the CIEP method.  No false positives were obtained in sera from 98 patients with other liver diseases or normal controls.  Both the latex and the CIEP tests must be performed on both undiluted and diluted sera to avoid prozone effects.  Simultaneous testing of samples with control latex for nonspecific rheumatoid-like agglutination is also necessary.  The latex method seems to be a simple, specific method of screening large numbers of sera for AFP;  all positive results should be confirmed by a control latex test and CIEP.  The method could be used for mass screening during pregnancy.


Separation of lipoprotein X and beta-lipoprotein by zonal centrifugation or hydroxyapatite chromatography.


The effect of phospholipases on the release of enzymes from intact cells.


Entamoeba histolytica: concurrent irreversible loss of infectivity-pathogenicity and encystment potential after prolonged maintenance in axenic culture in vitro.


Microphallus pygmaeus: effect of long-acting ACTH preparation on establishment and retention in alimentary canal of the mouse.


Inhibition of phosphodiesterase by dihydroergotamine and hydergine in various organs of the cat in vitro.


Stimulation of transcription in HEp-2 cells by UV-inactivated poliovirus type 1.


Effect of colchicine on long lasting synaptic modifications induced by hyperactivity.


A study of the cytology and cell culture of the canine transmissible veneral sarcoma.


The effect of kidney-bean leucoagglutinin on homograft rejection in mice.


Herpes-virus and double-stranded RNA.

Herpes-virus and double-stranded RNA.


Effect of triparanol (mer 29) on rat liver lysosomes: cytochemical and biochemical study.


Effects of cyclic AMP and theophylline on mitogen-induced RNA synthesis in human peripheral blood lymphocytes.


Fibrinolysis associated with oncogenic transformation. Requirement of plasminogen for correlated changes in cellular morphology, colony formation in agar, and cell migration. Fetal bovine and dog serum were selectively freed of plasminogen by affinity chromatography. The resulting serum as well as native and reconstituted serum (obtained by the addition of purified plasminogen to the plasminogen-depleted serum) were used to examine the role of plasminogen in (a) growth of normal and SV-40-transformed hamster embryo fibroblasts in liquid medium, (b) growth of SV-40-transformed hamster embryo fibroblasts in soft agar, (c) aggregation - a characteristic morphological change of SV-40-transformed hamster cells, and (d) migration of SV-40-transformed and control 3T3 cells from a monolayer into a "wound." The results demonstrated that exponential growth of both normal and transformed cells in liquid medium proceeded at the same rate in the presence or absence of plasminogen. In contrast, removal of plasminogen markedly depressed the plating efficiency of transformed cells in soft agar, eliminated their characteristic aggregation, and substantially reduced the extent of migration. The role of plasminogen and its activation in oncogenic transformation is discussed.


Evidence for a macrophage-mediated effect of poliovirus on the lymphocyte response to phytohemagglutinin.


The role of phosphorus restriction in the prevention of secondary hyperparathyroidism in chronic renal disease.


Role of humoral and cell-mediated immunity on the recovery of chickens from fowlpox virus infection.


Effects of lysozyme on normal and transformed mammalian cells.


Letter: Comments on "The administration of chiropractic in the New York City Medicaid program".


Influence of cyclic AMP on early events of the immune induction.


Should a paper on the administration of chiropractic have been published in Medical Care? With comments on derivative questions.


New bioassay for luteinizing hormone.

New bioassay for luteinizing hormone.


Proton magnetic resonance and the composition of proteins.


Changes in distribution of electric charges in the surface membrane of rat ascites hepatocarcinoma cells induced by con A.


Delta 9-tetrahydrocannabinol and the sleep-wakefulness cycle in rabbits.


Sleep patterns in the owl Strix aluco.

Sleep patterns in the owl Strix aluco.


The relationship between the antidiuretic activity of rat eye plexus blood and passive avoidance behaviour.


Amebiasis: newer methods of diagnosis and treatment.


Esophagogastric substitution after total gastrectomy and distal esophagectomy for locally advanced gastric cancer.


Oxandrolone and human growth hormone. Comparison of growth-stimulating effects in short children.


Studies on cartilage formation. XV. Enzyme histochemical investigation on Krebs cycle, terminal oxidation and hexose monophosphate shunt.


Cause of acute hemorrhagic cystitis in children.


Chronic idiopathic hyperphosphatasia in an Indian child.


Oral-facial dyskinesia and quadriplegia associated with poliomyelitis virus type 3.


Metastatic carcinoma to the pancreas. Another cause for acute pancreatitis.


Neighborhood Extension of Activity Therapy.


Avian cardiac receptors: activity changes by blood pressure, carbon dioxide, and pH.


Cardiac afferent activity in depressor nerve of the chicken.


Electrical discharge of single enteric neurons of guinea pig small intestine.


Effects of angiotensin I and angiotensin II on hindlimb and coronary vascular resistance.


Blast transformation of lymphocytes in amebiasis.


Epidemiology of the acute fevers of unknown origin in South Vietnam: effect of laboratory support upon clinical diagnosis.


Prostaglandins and the lung.

Prostaglandins and the lung.


Comparison of serum lipid and lipoprotein profiles of tuberculin-positive and tuberculin-negative monkeys (Macaca sp).


Letter: Herpes simplex of penis and anus.


Chemodectomas of the middle ear. A comparison of therapeutic modalities.


Comparative biochemical study of otosclerosis and osteogenesis imperfecta.


Isolation and characterization of surface membranes from chorioallantoic cells and chick fibroblasts.


ATPase in isolated membranes of Bacillus subtilis.


Letter: Methyldopa and depression.

Letter: Methyldopa and depression.


Suramin: a potent ATPase inhibitor which acts on the inside surface of the sodium pump.


A three year comparison of acute respiratory disease, shrink and weight gain in preconditioned and non-preconditioned Illinois beef calves sold at the same auction and mixed in a feedlot. During 1969 to 1971, 78 preconditioned (PC) and 79 non-preconditioned (NPC) beef calves were purchased at the same auction and mixed in a feedlot. Preconditioned calves were weaned 30 days before the sale, used to drinking from a tank, and vaccinated against blackleg, malignant edema, infectious bovine rhinotracheitis (IBR), parainfluenza-3 (PI3) and bovine virus diarrhea (BVD) in 1970 and 1971, and Pasteurella hemolytica and multocida in 1971. All vaccinations were completed two to three weeks before the sale. PC calves were given thiabenzadole. PC calves had significantly less shrink after shipment and in 1971 significantly more rapid daily gain during the first weeks of the feeding period. In 1969 more PC calves were treated for acute respiratory disease than NPC calves during an outbreak of PI3 and BVD infection. In 1970 and 1971 fewer PC than NPC calves were treated for acute respiratory tract disease during outbreaks of PI3 infection. The differences in clinical respiratory disease were significant in 1971. Inclusion of two doses of P. hemolytica and P. multocida bacterin before the sale in 1971 and use of an intranasal PI3 vaccine was considered to improve the PC program. Fecal egg counts for gastrointestinal nematodes were much lower in PC calves treated with thiabenzadole than untreated NPC calves.


Necrotic enteritis in broiler chickens. I. A review of the literature and the prevalence of the disease in Ontario. The available literature on necrotic enteritis in broiler chickens is reviewed. The records of three poultry diagnostic laboratories in Ontario were examined for the years 1969, 1970 and 1971. During this period 855 (7.7%) of 11,076 consignments of broiler chickens examined were diagnosed as necrotic enteritis. The condition was most common in broilers at three weeks of age, with 66% of all cases occurring in chickens between two and four and one half weeks. The disease occurred throughout the year but it was most common during July, August, September and October. Necrotic enteritis often occurred more than once per year on a farm.


Vesicular lesions produced in guinea pigs by a Staphylococcus aureus strain. A bacterium isolated from vesicular lesions on foot pads of guinea pigs reproduced lesions similar to those seen in experimental infections of guinea pigs with foot-and-mouth disease virus. (FMDV). These bacterial lesions were produced with an inactivated FMDV suspension. Identification as Staphylococcus aureus was determined by growth characteristics on nutrient and blood agar, Gram staining, fermentation of mannitol and coagulase positive reactions. In addition, the organism was sensitive to concentrations of penicillin and streptomycin commonly used in laboratory diluents.


Serum glycoproteins in hyperlipoproteinemic patients with and without clinical vascular disease.


The excretion of adenosine 3',5'-cyclic monophosphate by children with burns.


Catabolism of cholesterol in hypercholesterolemia and its relationship to plasma triglycerides.


Effect of exposure to quartz dust in vivo on pentose cycle activity in guinea pig lung.


Induction of 3T3 cell division at the monolayer stage. Early changes in macromolecular processes.


A radioautographic method for the localization of steroid ligands and receptors by cell affinity labelling.


Effects of N6-monobutyryl-cyclic AMP on glutamate decarboxylase activity in fetal rat brain cells and glial tumor cells in culture.


Temperature sensitive cells in the study of SV40 lysis versus SV40 transformation.


Subcellular localization of the leucine biosynthetic enzymes in yeast. When baker's yeast spheroplasts were lysed by mild osmotic shock, practically all of the isopropylmalate isomerase and the beta-isopropylmalate dehydrogenase was released into the 30,000 x g supernatant fraction, as was the cytosol marker enzyme, glucose-6-phosphate dehydrogenase. alpha-Isopropylmalate synthase, however, was not detected in the initial supernatant, but could be progressively solubilized by homogenization, appearing more slowly than citrate synthase but faster than cytochrome oxidase. Of the total glutamate-alpha-ketoisocaproate transaminase activity, approximately 20% was in the initial soluble fraction, whereas solubilization of the remainder again required homogenization of the spheroplast lysate. Results from sucrose density gradient centrifugation of a cell-free particulate fraction and comparison with marker enzymes suggested that alpha-isopropylmalate synthase was located in the mitochondria. It thus appears that, in yeast, the first specific enzyme in the leucine biosynthetic pathway (alpha-isopropylmalate synthase) is particulate, whereas the next two enzymes in the pathway (isopropylmalate isomerase and beta-isopropylmalate dehydrogenase) are "soluble," with glutamate-alpha-ketoisocaproate transaminase activity being located in both the cytosol and particulate cell fractions.


Purification and properties of Lactobacillus plantarum inducible malic enzyme. Inducible malic enzyme (l-malate:NAD oxidoreductase [decarboxylating], EC 1.1.1.39) was isolated from Lactobacillus plantarum and purified about 100-fold with 27% yield of the original activity. Kinetic studies with the purified malic enzyme yielded the following results: pH optimum, 7.6 to 8.0; K(m) for l-malate, 0.38 mM; K(m) for NAD, 0.072 mM; and K(m) for MnCl(2), 0.048 mM. It was shown that this enzyme was inhibited by high concentrations of substrate and nicotinamide adenine dinucleotide (NAD), indicating it may be regulated by substrate or NAD. Molecular weight of 130,000 +/- 10,000 was determined by Sephadex gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isoelectric point, determined by isoelectrofocusing, was 4.3 at 7 C. Isoelectrofocusing also resolved three active peaks which focused at pH 4.19, 4.31, and 4.40.


Isolation and characterization of two protease-producing mutants from Staphylococcus aureus. Two mutants with increased protease production were isolated after nitrosoguanidine treatment of Staphylococcus aureus 8325N. The wild type produces low amounts of extracellular proteolytic activity. The enzyme was inducible and could only be detected if casein or preferably skim milk powder was used as inducer. The optimal pH, salt concentration, and media for enzyme production were determined. The mutants differed from the wild type in several phenotypic characters. The pattern of extracellular deoxyribonuclease and alkaline phosphatase differed between the mutants and the wild type. Several carbohydrates such as lactose, galactose, and mannitol were not utilized by the mutants, probably owing to a block in the uptake. Glucose could, however, be utilized by the mutants. Reversion frequency to wild type with regard to carbohydrate utilization was spontaneously high, and all revertants regained the parental pattern irrespective of the carbohydrate used for selection. The results suggest that a single locus may control the excretion of extracellular enzymes and carbohydrate uptake in S. aureus.


Sensitization by ethylenediaminetetraacetate of Clostridium perfringens type A spores to germination by lysozyme. Clostridium perfringens spores (three strains) were normally resistant to germination by lysozyme. In the absence of disulfide bond-breaking reagents, tetrasodium ethylenediaminetetraacetate rapidly sensitized the spores to lysozyme.


Pyrimidine metabolism in Acinetobacter calcoaceticus. The metabolism of thymine, thymidine, uracil, and uridine has been investigated in five different strains of Acinetobacter calcoaceticus. Attempts to isolate thymine and thymidine auxotrophic mutants were not successful. Consistent with this finding was the observation that uptake of radioactive thymine or thymidine could not be demonstrated. Search for enzymes capable of transforming thymine via thymidine to thymidine-5'-monophosphate in crude extracts was performed, and the following enzymes were absent judging from enzyme assays: thymidine phosphorylase (EC 2.4.2.4), trans-N-deoxyribosylase (EC 2.4.2.6), and thymidine kinase (EC 2.7.1.21). The enzymes responsible for the phosphorylation of thymidine-5'-monophosphate to thymidine-5'-triphosphate were present in crude extracts. Radioactive uracil was readily incorporated into both ribonucleic acid and deoxyribonucleic acid, the ratio being 6:1, and radioactivity was found only in pyrimidine bases. No uptake of uridine could be demonstrated. Uridine-5'-monophosphate pyrophosphorylase (EC 2.4.2.9) activity was detected in crude extracts, suggesting that uracil is converted directly to uridine-5'-monophosphate which is then phosphorylated to uridine-5'-triphosphate or transformed to other ribo- and deoxypyrimidine nucleotides.


Isolation and characterization of a mutant of Salmonella typhimurium deficient in a major deoxyribonucleic acid polymerase activity. A mutant of Salmonella typhimurium strain LT2 that is deficient in a major deoxyribonucleic acid (DNA) polymerase activity has been isolated and characterized. This mutant resembles the pol mutants of E. coli in that it has low DNA polymerase activity and it is sensitive to methyl methane sulfonate as well as ultraviolet irradiation. Revertants selected for methyl methane sulfonate resistance are no longer sensitive to ultraviolet irradiation and contain normal DNA polymerase levels. No direct role in replication can be ascribed to this polymerase activity since cells grow well in its absence. In addition, the LT2 plasmid has been shown to exist in the mutant strain.


Trehalose metabolism in germinating spores of Streptomyces hygroscopicus. Evidence is presented which indicates that utilization of trehalose is an early event in spore germination in Streptomyces hygroscopicus. Early in spore germination and before any increase in cell mass, the activity of trehalase increased more than 15-fold, while the intracellular content of trehalose fell to very low levels. On the other hand, increased activity of the trehalose phosphate synthetase or disappearance of glycogen did not occur until later in germination, during which time cell mass was also increasing.


Evidence for proteolytic degradation of histidinol phosphate phosphatase specified by nonsense mutants of the hisB gene of Salmonella typhimurium. The gene products from hisB nonsense mutants having histidinol phosphate phosphatase activity were isolated from Salmonella typhimurium. The enzyme from strain TR691 (hisB278 hisT1529 aroD5) was isolated in the presence of diisopropylfluorophosphate. Three electrophoretically separable forms were demonstrated, and all were shown to have a mol wt of approximately 38,000 and to consist of a single polypeptide chain. Previously, two forms of the phosphatase enzyme from this strain were isolated without diisopropylfluorophosphate and shown to have a different subunit composition. Strain TA387 (hisB2133 his 01242) was shown to have two electrophoretically separable phosphatases with a mol wt of about 52,000 and consisted of 17,000- to 19,000-mol wt polypeptide chains as evidenced by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The results could be explained by proteolytic cleavage of the primary gene product which can partially be prevented by the protease inhibitor. Strain TA387 phosphatase lost all activity in 8 M urea but could be renatured by dialysis. Gel filtration showed that it also regained its original molecular weight. The values of K(m) of histidinol phosphate and the competition inhibition constant for histidinol were determined. The addition of MnCl(2) to the assay was shown to shift the optimal pH value to a lower pH value.


Altered mitochondrial respiration in a chromosomal mutant of Neurospora crassa. A mutant of Neurospora crassa (cni-1) has been isolated that has two pathways of mitochondrial respiration. One pathway is sensitive to cyanide and antimycin A, the other is sensitive only to salicyl hydroxamic acid. Respiration can proceed through either pathway and both pathways together in this mutant account for greater than 90% of all mitochondrial respiration. The cni-1 mutation segregates as a nuclear gene in crosses to other strains of Neurospora. Absorption spectra of isolated mitochondria from cni-1 show typical b- and c-type cytochromes but the absorption peaks corresponding to cytochrome aa(3) are not detectable. Extraction of soluble cytochrome c-546 from these mitochondria followed by reduction with ascorbate reveals a new absorption peak at 426 nm that is not present in wild-type mitochondria. This peak may be due to an altered cytochrome oxidase with abnormal spectral properties. Mitochondria from cni-1 have elevated levels of succinate-cytochrome c reductase but reduced levels of nicotinamide adenine dinucleotide reduced form cytochrome c reductase and of cyanide- and azide-sensitive cytochrome c oxidase. These studies suggest that the cni-1 mutation results in the abnormal assembly of cytochrome c oxidase so that the typical cytochrome aa(3) spectrum is lost and the enzyme activity is reduced. As a consequence of this alteration, a cyanide-insensitive respiratory pathway is elaborated by these mitochondria which may serve to stimulate adenosine 5'-triphosphate production via substrate level phosphorylation by glycolysis and the Krebs cycle.


Purification and properties of cytoplasmic and mitochondrial malate dehydrogenases of Physarum polycephalum. Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone fractionation of soluble plasmodial homogenates acidified to pH 5.0. The M-MDH was purified 201-fold by cetylpyridinium chloride treatment, fractionation with ammonium sulfate, gradient elution from sulfoethyl cellulose at pH 6.0, and Sephadex G-100 chromatography. The S-MDH was purified 155-fold by ammonium sulfate fractionation, diethylaminoethyl cellulose chromatography, gradient elution from sulfoethyl cellulose at pH 5.5, and Sephadex G-100 chromatography. The optimal cis-oxalacetate concentrations were 0.35 mM for M-MDH and 0.25 mM for S-MDH, and the optimal pH for both isoenzymes was 7.6 for oxalacetate reduction. The optimal l-malate concentrations were 5 mM for S-MDH and 6 mM for M-MDH, and both isoenzymes exhibited an optimal pH of 10.0 for L-malate oxidation. The Michaelis constants of S-MDH and M-MDH served to discriminate between the isoenzymes. The S-MDH was more heat-stable than the M-MDH. High concentrations of oxalacetate and malate inhibited S-MDH more than M-MDH. The isoenzymes were further distinguished by their utilization of analogues of nicotinamide adenine dinucleotide. Many properties of the Physarum isoenzymes were similar to those of more complex organisms, especially vertebrates.


Cyclic 3',5'-adenosine monophosphate phosphodiesterase of Escherichia coli. The cyclic 3',5'-adenosine monophosphate (c-AMP) phosphodiesterase from Escherichia coli has been partially purified. The enzyme has an apparent molecular weight of 30,000, a Michaelis constant of 0.5 mM c-AMP, and a pH optimum of 7. The partially purified enzyme requires for activity the presence of a reducing compound and of either iron or a protein which seemingly acts as iron carrier.


Ascorbate oxidase. Spectral characteristics of the enzyme.


Ultrastructural aspects of a mutant of Schizophyllum commune with continuous nuclear migration. Study of a mutant of the basidomycete Schizophyllum commune with continuous migration of nuclei revealed that the mutant is characterized by vacuolization, bundles of fibrillar-like material, and microtubules. The bundles of fibrillar-like material and microtubules extend through degraded septa to adjacent cells and are found in proximity to nuclei.


Mutants of Escherichia coli K-12 "cryptic," or deficient in 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (cyclic phosphodiesterase) activity. Mutants of Escherichia coli have been selected for the absence of 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (2',3'-cyclic phophodiesterase). Mutants selected for the absence of 5'-nucleotidase are of two kinds: those that lack detectable activity for the enzyme (Ush(-)), and those that possess activity when cell extracts are assayed, but not when intact cells are assayed (cryptic; Crp(-)). The latter class is probably identical to a type of mutant previously reported by Ward and Glaser. When mutants are selected for the absence of 3'-nucleotidase, Crp(-)mutants are also obtained. Thus far, however, mutants totally lacking this enzyme have not been found. The location on the genetic map of one ush mutation is at position 11 min and that of one crp mutation at approximately 67 min. In the crp mutant, 5'-nucleotidase and 3'-nucleotidase remain located in the periplasm. This mutant is also cryptic for alkaline phosphatase but not for acid hexose phosphatase. Treatment of cells with ethylenediamine-tetraacetate substantially alleviated crypticity. These data are discussed in terms of the organization of periplasmic enzymes and of the outer membrane as a permeability barrier.


Expression of genes for metabolism of cyclic adenosine 3':5'-monophosphate in somatic cells. I. Responses to catecholamines in parental and hybrid cells.


Expression of genes for metabolism of cyclic adenosine 3':5'-monophosphate in somatic cells. II. Effects of prostaglandin E1 and theophylline on parental and hybrid cells.


Cu (II)-carbon bonding in cyanide complexes of copper enzymes. 13C splitting of the Cu(II) electron spin resonance.


High spin and low spin forms of Co(II) carbonic anhydrase. Temperature-dependent changes in spin state and coordination geometry.


The nucleotide sequence of a serine transfer ribonucleic acid from Escherichia coli.


Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells.


Capacity for steroidogenesis of adrenals in hypophysectomized and adrenocorticotropic hormone-treated hypophysectomized rats. Implication of a ribonucleic acid for hormone-induced steroidogenesis.


Comparison of the effects of adrenocorticotropic hormone on the steroidogenic activity and ultrastructure of adrenal cortex.


1,24,25-Trihydroxyvitamin D3. A metabolite of vitamin D3 effective on intestine.


Chemical synthesis of delta 7,24-(3alpha-3H)cholestadien-3beta-ol and its conversion to cholesterol in the rat.


Positional specificity of purified milk lipoprotein lipase.


The variety of intraspecific misacylations carried out by isoleucyl transfer ribonucleic acid synthetase of Escherichia coli.


A role for the microtubular system in the release of very low density lipoproteins by perfused mouse livers.


Synthesis and antiviral activity of homologs of noformycin.


Control of steroidogenesis in pre-ovulatory cells. Luteinizing hormone stimulation of (14C)-acetate incorporation into sterols.


Angiotensin II. Synthesis and biological activity of 6-(1-methylhistidine) and 6-(3-methylhistidine) analogs.


Effect of dietary protein and magnesium on selected ATP phosphotransferases and neuromuscular behavior in young rats.


Occult carcinoma of second breast following mastectomy.


Phospholipids and calcium uptake in experimental tumors.


Effects of dibutyryl cyclic AMP and related compounds on newt limb regeneration blastemas in vitro. I. 3H-thymidine incorporation.


Effects of dibutyryl cyclic AMP and related compounds on newt limb regeneration blastemas in vitro. II. 14C-leucine incorporation.


Somatosympathetic reflexes: afferent fibers, central pathways, discharge characteristics.


Neural processes in thermoregulation.

Neural processes in thermoregulation.


Amoebic infection of the female genital tract. A report of three cases.


Phosphorylation of muscle membranes: identification of a membrane-bound protein kinase. A membrane-bound protein kinase occurs in membranes derived from rat skeletal muscle and appears limited to a surface membrane fraction. The enzyme is magnesium dependent, is only minimally stimulated by cyclic nucleotides, and phosphorylates serine and to a lesser extent threonine residues of three membrane proteins with molecular weights of less than 30,000.


Pancreatitis and hyperlipoproteinemia.

Pancreatitis and hyperlipoproteinemia.


Oscillation of cyclic adenosine monophosphate concentration during the myocardial contraction cycle. The concentration of adenosine 3',5'-monophosphate (cyclic AMP) rises and falls during each myocardial contraction cycle. Peak concentrations of cyclic AMP precede peak development of systolic tension. Epinephrine alters the normal oscillation in myocardial cyclic AMP and increases both diastolic and systolic concentrations of the cyclic nucleotide. These transient changes in myocardial cyclic AMP indicate a potential role for cyclic AMP as a beat-to-beat regulator of myocardial contractility.


Differences in the response of normal and transformed BHK21 cells to dual virus infection. Conditions affecting synergism between vesicular stomatitis virus and newcastle disease virus.


Presence of antigen common to avian tumor viral envelope antigen in normal chick embryo cells.


Protein metabolism in SV40-infected cells.


Specificity of TMV RNA encapsidation: in vitro coating of heterologous RNA by TMV protein.


Evidence for helper independent murine sarcoma virus. I. Segregation of replication-defective and transformation-defective viruses.


Effects of omentumectomy on production of endotoxin-type interferon in mice.


Diffusion coefficients and hydrodynamic radii of three spherical RNA viruses by laser light scattering.


Differential expression of transformation in rat and chicken cells infected with an avian sarcoma virus ts mutant.


Effects of dibutyryl-cAMP on adenovirus replication in different cell lines.


Synthesis of complementary RNA by poliovirus polymerase in vitro.


Size of gene sequences shared by polioviruses types 1, 2, and 3.


Analysis of the inhibition of the ACTH release by hypothalamic implants of atropine.


Stimulation of ornithine decarboxylase and nuclear RNA polymerase activity in rat liver by glucagon and dibutyryl cyclic amp.


Effect of D2O and nicotinamide adenine dinucleotide on the sedimentation properties and structure of glyceraldehyde phosphate dehydrogenase.


Nuclear magnetic resonance studies of the interaction of valinomycin with unsonicated lecithin bilayers.


Isolation and characterization of the cyanogen bromide fragments from the high-density apolipoprotein glutamine I.


Functional arginyl residues in carboxypeptidase A. Modification with butanedione.


Competitive inhibitors of renin.

Competitive inhibitors of renin.


Interaction of actin with analogs of adenosine triphosphate.


Properties of tRNALys4 from various tissues.


Correlation between a specific isoaccepting lysyl transfer ribonucleic acid and cell division in mammalian tissues.


Nature and possible functions of association between glutamate dehydrogenase and cardiolipin.


Amino acid sequence of a cytochrome c from the common Pacific lamprey, Entosphenus tridentatus.


Uridylic acid synthesis in Ehrlich ascites carcinoma. Properties, subcellular distribution, and nature of enzyme complexes of the six biosynthetic enzymes.


Some properties of cross-linked polymers of glutamic dehydrogenase.


Synthesis and characterization of photoaffinity labels for adenosine 3':5'-cyclic monophosphate and adenosine 5'-monophosphate.


On the photoaffinity labeling of rabbit muscle phosphofructokinase with O2'-(ethyl-2-diazomalonyl)adenosine 3':5'-cyclic monophosphate.


Fluorescence properties of octopine dehydrogenase.


Independent protein synthesis in isolated rat tumor nucleoli. Aminoacylation of endogenous transfer ribonucleic acid.


Characterization of modified myosin at low ionic strength. Enzymatic and spin-label studies.


Nuclear magnetic resonance and spin-label studies of hemoglobin Kempsey.


Immune response to the collagen-like synthetic ordered polypeptide (L-Pro-Gly-L-Pro)n.


Heterogeneity of the outer membrane of mitochondria.


Microcalorimetric study of isolated blood platelets in the presence of thrombin and other aggregating agents.


Preparation and active-site specific properties of sturgeon muscle glyceraldehyde-3-phoshate dehydrogenase.


Human chorionic gonadotropin-mediated glycogenolysis in human placental villi: a role of prostaglandins.


Factors affecting the adrenocorticotropic hormone stimulation of rabbit adrenal 17alpha-hydroxylase activity.


Prolonged retention of high specific activity by 125I-labeled angiotensin II--a consequence of 'decay catastrophe'.


Photolumazines, new naturally occurring inhibitors of riboflavin synthetase.


Ionic control of renal gluconeogenesis. II. The effects of Ca2+ and H+ upon the response to parathyroid hormone and cyclic AMP.


Ionic control of renal gluconeogenesis. IV. Effect of extracellular phosphate concentration.


Endogenous ACTH induced remission in myasthenia gravis.


Evidence for neuropathy in myotonic muscular dystrophy.


The effect of diazoxide in children with islet cell adenomas.


The effect of stress induced by ether anaesthesia on cholesterol content and cholesteryl-esterase activity in rat-adrenal cortex.


Baldness: a disease?

Baldness: a disease?


Epidemiological factors in lung cancer among women in El Pasco County, Texas 1944-1969.


Women's programs at DHEW.

Women's programs at DHEW.


Women doctors educating others. 50th anniversary of the American Medical Women's Association Scholarship Loan Fund.


Action of Vibrio cholerae neuraminidase (sialidase) upon the surface of intact cells and their isolated sialolipid components.


Extensive incorporation of (2-14C) mevalonic acid into cholesterol precursors by human platelets in vitro.


Methionyl residue critical for activity and regulation of bovine liver glutamate dehydrogenase.


Effect of exercise on the turnover of cytochrome c in skeletal muscle.


Effects of insulin on the levels of adenosine 3':5'-monophosphate and lipolysis in isolated rat epididymal fat cells.


Iron and free radical in ribonucleotide reductase. Exchange of iron and Mössbauer spectroscopy of the protein B2 subunit of the Escherichia coli enzyme.


Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli.


Liver cytosol corticosteroid binder II, a hormone receptor.


Deoxyribonucleic acid ligase. A steady state kinetic analysis of the reaction catalyzed by the enzyme from Escherichia coli.


Metalloflavoenzyme models. The crystal structure of bis (10-methylisoalloxazine) copper (II) perchlorate tetrahydrate.


Alkylation of an essential histidine residue in porcine spleen deoxyribonuclease.


Physical properties of a purified cyclic adenosine 3':5'-monophosphate-dependent protein kinase from bovine heart muscle.


Receptor for thyrotropin-releasing hormone in plasma membranes of bovine anterior pituitary gland. Role of lipids.


Preliminary report on the electron paramagnetic resonance spectra of single crystals of bovine erythrocyte superoxide dismutase.


Determination of aza-heterocyclic compounds in atmospheric dust by a combination of thin-layer and gas chromatography.


Role of adrenal hormones in incubation behavior of male ring doves (Streptopelia risoria).


Synthesis and antiviral activity of 1,2,4-triazole-3-thiocarboxamide and 1,2,4-triazole-3-carboxamidine ribonucleosides.


Synthesis of 5'-phosphates of the naturally occurring 6-ureidopurine ribonucleosides and their analogs.


Different cyclic AMP requirements for induction of the arabinose and lactose operons of Escherichia coli.


RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template.


Recurrence of the cytochrome fold in a nitrate-respiring bacterium.


Mutagenic specificity: reversion of iso-1-cytochrome c mutants of yeast.


Public health implications of Marek's disease virus and herpesvirus of turkeyes. Studies on human and subhuman primates.


Preparation of membrane fractions with enhanced tumor-transplantation-antigen activity from tumor cells infected with influenza virus.


Lymphomas in BALB-c mice inoculated with supernatants from chemically induced sarcomas.


Differential response of rat tooth germs to a murine sarcoma virus in cell culture and syngeneic transplants.


Prevalence of type-C particles in visceral tissues of embryonic and newborn mice.


XC cell cytopathogenicity as an assay for murine myeloma C-type virus.


A spontaneous lower motor neuron disease apparently caused by indigenous type-C RNA virus in wild mice.


Rous sarcoma virus-induced tumors in marmosets: characteristics of tumor cells and host cell-virus interrelationships.


Effect of streptozotocin in the Chinese hamster (Cricetulus griseus).


Brief communication: In vitro host range and serologic studies on RD-114 virus.


Brief communication: circulating antigen-antibody complex associated with Epstein-Barr virus in recurrent Burkitt's lymphoma.


Stereochemistry in the solvolytic ring contraction of 2,2,4a alpha-trimethyl-1-decalyl methanesulfonate. A model reaction pertaining to triterpene biogenesis.


Skin test in bronchogenic carcinoma. I. Correlation of the immunological status and the extent of the disease.


Pseudomonas meningitis complicating radical resection for radiorecurrent cancer of the paranasal sinuses: report of two patients successfully treated with intrathecal polymyxin.


Cross-classification and survival characteristics of 5,000 cases of cancer of the pancreas.


Water pollution: microbiology of water.

Water pollution: microbiology of water.


Managing member silence in family therapy.


Implications of the incest taboo for nursing practice.


Radiotherapy of adenoid cystic carcinoma of the head and neck.


Microbiology--detection and occurrence of viruses.


The CVA patient as an indication of general patient need.


Philosophy and structure of a day treatment center.


The role of science in the World Health Organization.


Speaking out on: professional rejection of the longer-term patient??


Problems of a nurse group on an inpatient psychiatric unit.


Management of alleged sexual assault.

Management of alleged sexual assault.


A comparative follow-up of functional psychoses in women.


Food for the elderly.

Food for the elderly.


Rapid new soft x-ray technique for determination of quartz in coal mine dusts.


Epidemiology of respiratory syncytial virus infection in Washington, D.C. 3. Composite analysis of eleven consecutive yearly epidemics.


Organization of emergency services: the crisis nurse in the community mental health center.


Adrenocortical function in acromegaly.

Adrenocortical function in acromegaly.


The road to Shangri-La is paved with vitamins.


Antagonism of d-tubocurarine- and pancuronium-induced neuromuscular blockades by pyridostigmine in man.


Halothane stereoisomers: lack of stereospecificity in two model systems.


Letter: Standardized technique for transmission studies.


Postoperative endophthalmitis following cataract surgery. Effects on subconjunctival antibiotics and other factrs.


Endocrine spotlights on brain and behaviour.


Chronic ulcerative keratitis caused by herpes simplex virus.


An insoluble fibrogenic factor in macrophages from guinea pigs exposed to silica.


Noradrenergic pathways and sleep-waking states in cats.


Polarization changes in the terminals of identified primary afferent fibers at the gracile nucleus.


Studies on single neurons in dorsal hippocampal formation and septum in unrestrained rats. I. Behavioral correlates and firing repertoires.


Molybdenum(IV) in aqueous solutions.

Molybdenum(IV) in aqueous solutions.


Dysfunction patterns analogous to frontal lobe system and caudate nucleus syndromes in some groups of minimal brain dysfunction.


Is anatomy destiny? Panel: the changing role of women psychiatrists.


New Jersey women physicians--their opinions on today's world.


Hypertension in terminal renal failure. Observations pre and post bilateral nephrectomy.


Renovascular hypertension: incidence, diagnosis, mechanism and treatment.


Antagonism of vasoconstrictor responses by exercise in the gracilis muscle of the dog.


A new technique for treating esophageal varices.


Nephroblastoma: index cancer of childhood.


Low back pain. Differential diagnosis determines treatment: hospitalization enables more efficient treatment.


Morphine tolerance and supersensitivity to 5-hydroxytryptamine in the myenteric plexus of the guinea-pig.


Vitamin D-like action of Solanum malacoxylon on calcium transport by rat intestine.


Stannous pyrophosphate labeled with technetium-99m for skeletal scintigraphy.


Effect of cyclic AMP on growth and morphological differentiation of an established myogenic cell line.


Agglutination with con A dependent on cell cycle.


Depletion of serum growth factors by 3T3 mouse fibroblasts and viral transformants.


Adrenaline-like effects of intracellular iontophoresis of cyclic AMP in cardiac Purkinje fibres.


NMR evidence against gamma turn and beta turn models of angiotensin II in aqueous solution.


Electronic sector scanning in the diagnosis of cerebrovascular disease and space-occupying processes.


Serum immunoglobulin and complement (C3) levels: a study in adults with idiopathic, chronic polyneuropathies and motor neuron diseases.


The effect of cold on nerve conduction of human slow and fast nerve fibers.


Studies on the synaptic facilitatory action of nikethamide.


Electroencephalographic study of the effects of tetrahydrocannabinols on sleep in the rat.


Functional organelles in prokaryotes: polyhedral inclusions (carboxysomes) of Thiobacillus neapolitanus. The polyhedral inclusions of Thiobacillus neapolitanus have been isolated; they contain ribulose diphosphate carboxylase.


Rapid viral induction of plasmacytomas in pristane-primed BALB-c mice. Strain BALB/c mice were injected intraperitoneally with 0.5 milliliter of pristane, and 39 to 56 days later they were infected with Abelson murine leukemia virus, which is a lymphosarcomagenic variant of Moloney virus. Fifty-eight percent of the mice developed lymphosarcoma, and 28 percent developed immunoglobulin-producing plasmacytomas within 20 to 93 days (77 to 149 days after the pristane injection). Two of 57 control mice developed plasmacytomas at days 138 and 166 after a single injection of pristane; no plasmacytomas were found in mice treated with virus alone.


Collagen synthesis: localization of prolyl hydroxylase in tendon cells detected with ferritin-labeled antibodies. An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.


Gastric acid secretion in insulin-secreting tumors of the pancreas.


Isolation of viable cells from experimental granulation tissue.


IgM-producing malignant lymphomas without macroglobulinemia. Morphological and immunochemical findings.


A histochemical study of the inclusion bodies in the small intestine of the developing rat.


The structure of the gill of the trout, Salmo gairdneri (Richardson).


Herpesvirus encephalitis following herpes keratitis.


Effects of colchicine and vinblastine on neurotubules of the sciatic nerve and cholinesterases in the developing myoneural junction of the rat.


Evidence for basal secretion in the subcommissural organ of the adult rabbit.


Intracranial arterial occlusion in children. Diagnosis and follow-up by brain scanning.


Congenital anomalies and herpesvirus infection.


Vasoconstriction-volume analysis for understanding and treating hypertension: the use of renin and aldosterone profiles.


Searching out low renin patients: limitations of some commonly used methods.


Renin, angiotensin and hypertensive vascular damage: a review.


The control of renin release.

The control of renin release.


Neurogenic factors in human hypertension: mechanism or myth?


Hypertension in chronic renal failure. An abnormal relation between sodium and the renin-angiotensin system.


Cushing's syndrome caused by recurrent malignant bronchial carcinod. Case report with 12 years' observation.


An experimental study of the effects of drains on colon anastomoses.


Pressure synthesis of thioformamide.

Pressure synthesis of thioformamide.


Homogeneous catalysis by transition metal complexes. 3. A new cobalt-cyanide catalyst for selective homogeneous hydrogenation of dienes.


Mechanism of the antihypertensive effect of 1-Sar-8-Ala-angiotensin II during the acute phase of experimental renal hypertension.


Dopamine-induced neurogenic vasodilatation: antagonism at the vascular level.


Ocular response to corticotropin in myasthenia gravis.


Influence of vasoactive hormones on blood flow through the mammary artery in lactating cows.


Malignant mixed tumor of the lacrimal gland. Occult source of metastatic carcinoma.


The role of vitamin D metabolites in bone resorption.


Repolarization-induced reactivation of contracture tension in frog skeletal muscle.


Influence of acute stimuli on plasma aldosterone concentration in anephric man and kidney allograft recipients.


Pharmacologic receptor activity of rabbit aorta. Effect of dithiothreitol and N-ethylmaleimide.


A rapid competitive protein-binding assay of unconjugated oestradiol in late pregnancy plasma.


Determination of urinary amino acids by gas chromatography.


Biochemical properties of normal and neoplastic cell surfaces; a review.


Polyacrylamide gel electrophoresis as a method for differentiation and quantitation of serum enzymes hydrolysing phosphate monoesters.


The cytotoxicity of chlorambucil and its associated effects on NAD metabolism.


Wilm's tumour treated at the Rotterdam Radiotherapy Institute (1964-1971).


Standards for the assessment of estrogen receptors in human breast cancer. Report of a workshop on September 29, 1972, at the Antoni van Leeuwenhoek-Huis, Amsterdam.


Content of estrogen receptors in human breast cancer.


Induction of stimulus-bound repetition in sympathetic ganglia by germine-mono-acetate.


The effects of PGE1 on responses to cardiac vagus nerve stimulation and acetylcholine release.


Cyclic adenosine 3',5'-monophosphate concentration in rabbit parotid slices following stimulation by secretagotues.


Prostaglandin release from vasculature by angiotensin II: dissociation from lipolysis.


Synaptic biochemistry of amino acids.

Synaptic biochemistry of amino acids.


Studies of human amebiasis. II. Light and electron-microscopic observations of colonic mucosa and exudate in acute amebic colitis.


Rapid serological methods for the demonstration of Entamoeba histolytica activity.


Studies of human amebiasis. I. Clinical and laboratory findings in eight cases of acute amebic colitis.


Letter: Effect of beta stimulant on eosinopenic responses.


Neurite development in vitro. II. The role of microfilaments and microtubules in dibutyryl adenosine 3',5'-cyclic monophosphate and nerve growth factor stimulated maturation.


Chlorpromazine: effects of antacids on its gastrointestinal absorption.


Experimental inhibition of myelination in spinal cord tissue cultures: enzyme assays.


Fasting hypoglycemia.

Fasting hypoglycemia.


Acute renal failure.

Acute renal failure.


The response to chemical mutagens of the individual haploid and homoallelic diploid UV-sensitive mutants of the rad 3 locus of Saccharomyces cerevisiae.


Cytoplasmic proteins regulating messenger RNA release from nuclei.


RNA-directed cell-free synthesis of the galactose enzymes of Escherichia coli.


Discontinuous elongation of both strands at the replication forks in polyoma DNA replication.


Immunospecificity to non-histone chromosomal proteins of anti-chromatin antibodies.


The accumulation of 32P-polyphosphates of calcium, magnesium, lanthanum and sodium by tumor bearing animals: its radiotherapeutical significance.


Photochemical labeling of yeast alcohol dehydrogenase with an azide analog of NAD+.


Effect of polymyxin B and endotoxin on renal cortical fibrinolysis.


Evidence for glycosylated crosslinks in body-wall collagen of the sea cucumber, Thyome briareus.


Acid liability of rhinovirus type 14: effect of pH, time, and temperature.


The role of adenine nucleotides and the effect of caffeine and dibutyryl cyclic AMP on the metabolism of guinea pig epididymal spermatozoa.


Energy transfer between nucleic acid bases and tryptophan in aggregates and in oligopeptide-nucleic acid complexes.


The effects of metyrapone and ACTH on the development of gluconeogenesis in the neonatal pig.


Heterologous activity of monkey interferons.


Nicotinamide adenine dinucleotide (NAD) content of liver with hemorrhagic shock.


Influence of preinfection of C57BL-L mice with Graffi leukemia virus on 3-methylcholanthren-induced subcutaneous sarcoma.


Abortive herpes simplex virus replication in Rous sarcoma virus transformed cells.


Pathogenetic studies of herpes simplex virus infection of the rabbit eye.


Incorporation of a phospholipid precursor into the subcellular organelles of 9-methylpteroylglutamic acid-treated rat embryos.


Enzymes of arginine and urea synthesis.

Enzymes of arginine and urea synthesis.


The amidotransferases.

The amidotransferases.


Parainfluenza-3 and bovine enteroviruses as possible important causative factors in bovine abortion.


Immunogenicity of canine distemper virus vaccine produced in a continuous cell line (LLC-MK2 cells).


Bile duct carcinoma in cattle: three case reports.


Correlation between TS phenotype and pathogenicity of some animal viruses.


Studies on reovirus type 2: viral interference with UV-inactived virus.


The effect of different media on maintenance of chick embryo tracheal organ cultures and replication of influenza virus. Brief report.


The influence of dipyridamol on changes in the heart induced with posterior pituitary extract.


Case report of a familial oxalosis (ultrastructural study on the pathogenesis of kidney changes).


Effect of a single injection of betamethasone disodium phosphate on the synthesis of collagen and noncollagen protein of carrageenin granuloma in rats.


Effect of antimycin A on hormone-stimulated lipolysis in vitro.


Localization and characterization of the inhibitory action of ethacrynic acid on glycolysis.


Identification of (-)-delta 9-6a,10a-trans-tetrahydrocannabinol and two of its metabolites in rats by use of combination gas chromatography-mass spectrometry and mass fragmentography.


The mode of action of beta-benzal butyric acid, an hypocholesterolemic drug, in affecting mitochondrial respiration.


Effect of hydrocortisone acetate, fluocinolone acetonide, fluclorolone acetonide, betamethasone-17-valerate and fluprednyliden-21-acetate on collagen biosynthesis.


Reaction of cytochrome c with one-electron redox reagents. I. Reduction of ferricytochrome c by the hydrated electron produced by pulse radiolysis.


Hepatic free radical levels in ethanol-treated and carbon tetrachloride-treated rats.


CO-binding pigments and the functional terminal oxidase of the trypanosomatid hemoflagellate Crithidia fasciculata.


Electron spin resonance characterization of Chromatium D hemes, non-heme irons and the components involved in primary photochemistry.


Nucleoside transport in normal and polyoma-transformed cells: kinetic differences following adenosine and serum or insulin stimulation.


Purification and properties of a nucleolar endoribonuclease from Novikoff hepatoma.


A rapid high yield method for the preparation of rat liver cell plasma membranes.


Oxidative phosphorylation in immature rat brain mitochondria.


The lipid composition of plasma lipoprotein density classes of sheep Ovis aries.


Cyclic nucleotide phosphodiesterase from Tetrahymena.


Health planning as a managerial ideology.


Aspects of adrenal function in neonatally estrogenized male mice.


Active transport of 131-I across the blood-brain barrier. The ventricular space of rabbits was perfused with a low-viscosity silicone oil for the purpose of (1) collecting freshly secreted cerebrospinal fluid (c.s.f.) uninfluenced by diffusional exchanges with the brain and (2) studying passage of solutes from the blood into the brain, uncomplicated by exchanges with c.s.f. The freshly secreted c.s.f. appeared as fine droplets suspended in the less dense silicone, and accumulated at the bottom of the collected silicone. Studies on the penetration of (24)Na from blood into this fluid indicated that considerable exchanges with the brain had occurred between its secretion and collection, in spite of this method of collection. The second objective was attained, in that the exchanges between the freshly secreted fluid and the brain were quantitatively insufficient to affect the measure of kinetics of uptake by brain from the blood. In consequence, it was possible to demonstrate unequivocally that the increased uptake by brain of (131)I, when treated with perchlorate, was due to inhibition of an active process occurring across the blood-brain barrier. Other studies, involving ventriculo-cisternal perfusion with artificial c.s.f., lent further support to this concept. (131)I distribution is some 32% of the brain weight, a figure close to the ;chloride-space'.


On the mechanism of halothane anaesthesia. 1. The effects of halothane on the evoked potentials of in vitro preparations of guinea-pig olfactory cortex were studied.2. The evoked potentials recorded from the cortical surface comprised an initial diphasic wave - the lateral olfactory tract (l.o.t.) compound action potential - followed by a negative wave of 1-3 mV amplitude and about 10 msec duration. Superimposed on the negative wave was a number of positive peaks. The negative wave has been identified as an extracellularly recorded, monosynaptic, excitatory post-synaptic potential (e.p.s.p.) and the positive peaks have been shown to reflect the discharge of the cortical cell population in response to the evoked e.p.s.p. and are therefore termed ;population spikes'.3. When halothane (0.4-1.5%) was added to the gas stream that superfused the surface of the preparation the evoked e.p.s.p.s became smaller in amplitude and the size of the population spikes diminished. The l.o.t. compound action potential was unaffected by these levels of halothane. Higher levels of halothane (above 2%) further reduced the amplitude of the evoked e.p.s.p.s, abolished the population spikes, decreased the amplitude of the l.o.t. compound action potential and slowed its time course. The effects of halothane on the evoked potentials were dose-related and were independent (after the first 10 min of treatment) of the duration of the exposure to halothane.4. The decrease in the size of the population spike caused by the exposure to halothane implied that transmission through the cortical relay had been impaired. This was also shown by the decrease in the evoked activity of units in the prepiriform cortex. Of eleven units, eight were depressed by halothane (0.5-1.5%) two were unaffected and one showed a transient increase in the number of spikes generated in response to a l.o.t. volley.5. Halothane (up to 1.5%) had no effect on the threshold of the l.o.t. fibres to electrical stimulation or on that of the post-synaptic cells to synaptic excitation.6. Post-tetanic potentiation and frequency potentiation of the evoked e.p.s.p.s were enhanced in the presence of 1% halothane.7. It is concluded that halothane reduces excitatory synaptic transmission not by an increase in the electrical threshold of the post-synaptic cells to synaptic excitation but by interference with the process of chemical transmission either by reducing the output of transmitter from the pre-synaptic nerve terminal or by reducing the sensitivity of the post-synaptic membrane to the released transmitter substance.


Effect of insulin and epinephrine on the carbohydrate metabolism and adenylate cyclase activity of rhesus fetal muscle.


Suppression of growth of L cells in suspension culture by dibutyrl adenosine 3',5'-monophosphate.


Subcellular constituents of human placenta. I. Isolation and characterization of lysosomes from term tissue.


Inhibitory effect of streptonigrin on a murine sarcoma virus-induced tumor cell line (MSC) and selection of drug-resistant clones.


Selective detachment of mitotic cells by hypotonic salt solution.


Steroids and steroidases XX (1). Aggregation in aqueous solution of steroids with stigmastane type C-17 side chains and its influence on their enzymic transformations.


Synthesis of 21-3H-corticosteroids.

Synthesis of 21-3H-corticosteroids.


Biosynthesis of steroids from cholesterol-14C by human adrenal carcinoma tissue.


The renin axis in causation, prognosis and treatment of hypertensive disease.


The carcinoembryonic antigen (CEA) in carcinomata of the stomach.


Effect of ovarian hormones on lipid metabolism in different tissues of rats.


Effect of imidazole on the adrenal response to ACTH and to stress.


Investigations on the correlation between abnormalities of neuromuscular transmission due to some organophosphates and activity of acetylcholinesterase in the skeletal muscle.


The effects of prostaglandin F2 alpha on the structure and function of the rabbit ovary.


Morphological changes in the corpus luteum correlated with progestin levels in the rhesus monkey during early pregnancy.


Mouse bone collagenase. The effect of heparin on the amount of enzyme released in tissue culture and on the activity of the enzyme.


Selective inactivation of the infectivity of freeze-dried Sendai virus by heat.


Propionin B, an antiviral principle of propionibacteria.


Influence of plasma on ACTH stimulated corticosterone production of isolated adrenal cells.


Clinical pharmacology of sleep: a critical review of all-night electroencephalographic studies.


Rhabdoviruses.

Rhabdoviruses.


Viruses with double-stranded RNA genomes.


A spin-label study of the role of phospholipids in the regulation of membrane-bound microsomal enzymes.


Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.


A map of simian virus 40 transcription sites expressed in productively infected cells.


Letter: Electron microscopic observation of DNA condensates at low pH values.


Adenovirus isolation from recurrent oral ulcers.


Amoebiasis cutis.

Amoebiasis cutis.


Discharge and a discharge group.

Discharge and a discharge group.


Students learn to negotiate for role and demonstrate skill as group.


Correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mRNA translation in noninfected L cells. When noninfected L-cell suspension cultures are treated with interferon (specific activities superior to 10(6) reference units per mg of protein), the cell-free cytoplasmic extracts obtained are inactive for the translation of exogenous natural mRNAs. The dose-response curve shows that comparable amounts of interferon are required to produce a 50% reduction of Mengo virus multiplication in vivo and Mengo RNA translation in vitro. With higher doses of interferon, Mengo RNA translation is completely abolished, while poly U translation and endogenous protein synthesis are only slightly affected. The inactivation of Mengo RNA translation is reversible; after removal of interferon, normal translation activity is regained together with the ability to support Mengo virus multiplication. Fractionation of the cell-free extracts shows that the effect is localized in the fraction which can be washed off the ribosomes by high salt. These results establish that interferon induces a block in genetic translation in noninfected L cells.


Preventive aspects of the comprehensive psychiatric service in USSR.


Evaluating mental health status with a simplified rating scale.


Acquisition of sequences homologous to host DNA by closed circular simian virus 40 DNA. II. Further studies on the serial passage of virus clones. Three plaque isolates of SV40 strain 777 and 1 plaque isolate of strain 776 were grown to high-titer stocks and serially passaged, undiluted, in monkey BS-C-1 cells. In each case, the serial passaging procedure resulted in the accumulation of closed-circular SV40 DNA molecules containing covalently linked sequences homologous to reiterated host cell DNA (called substituted virus DNA). The relative yields, at a given passage level, of SV40 DNA with measurable homology to host DNA varied in different sets of serial passages, including passages of the same virus clone. More reproducible yields of substituted viral DNA progeny were obtained when the serial passaging procedure was initiated from earlier passages rather than from the original plaque-purified stock. Fractionation of closed-circular SV40 DNA molecules on alkaline sucrose gadients indicated that the majority of substituted virus DNA molecules are not plaque producers and are slightly smaller in size than plaque-forming DNA molecules which display no detectable homology to host DNA. Evidence that substituted SV40 DNA molecules replicate during serial undiluted passage was obtained from experiments which demonstrated (i) the presence of host sequences in replicative forms of the viral DNA and (ii) the incorporation of (3)H-thymidine into host sequences isolated from the mature substituted virus DNA molecule.


The "sectorization" of comprehensive psychiatric services in France.


Chromatographic analyses of isoaccepting tRNAs from avian myeloblastosis virus. Avian myeloblastosis virus (AMV) 4S RNA was tested for amino acid acceptor activity for 18 of the 20 amino acids. A nonrandom distribution of viral tRNAs was found compared with tRNA from normal liver or from AMV-infected leukemic myeloblasts, confirming previous reports. Methionine and proline tRNAs were considerably enriched, whereas glutamic acid, glutamine, serine, tyrosine, and valine tRNAs were markedly depleted in AMV relative to homologous cellular tRNAs. The seven AMV tRNAs with the greatest amino acid acceptance capacities, which were in order methionine, proline, lysine, arginine, histidine, isoleucine, and threonine tRNAs, were compared with homologous tRNAs from leukemic myeloblasts and liver by reversed-phase 5 chromatography. Of the 25 isoaccepting chromatographic fractions identified, no tRNA species unique to AMV was detected. Only methionyl-tRNA showed a substantial quantitative variation in isoaccepting species compared with the host cell. Thus, viral selectivity for amino acid-specific tRNAs is not, generally, paralleled by selectivity for individual isoaccepting tRNA species. Qualitative differences in arginyl- and histidyl-tRNA isoaccepting species were discovered in virus and leukemic myeloblasts compared with liver. This indicates the existence of structural differences in these tRNA species which could be related to virus replication or expression.


Introduction of goal-oriented record keeping on an inpatient drug treatment setting.


A forced-choice test for selecting psychiatric personnel.


Meeting basic needs of the elderly.

Meeting basic needs of the elderly.


Acquisition of sequences homologous to host DNA by closed circular simian virus 40 DNA. 3. Host sequences. A preparation of serially passaged simian virus 40 (SV40) DNA, in which at least 66% of the molecules contain covalently linked cellular DNA sequences, was digested to completion with the Hemophilus influenzae restriction endonuclease. Polyacrylamide gel electrophoresis of the digest showed that the majority of the cleavage products migrated as nine classes of fragments, each class defined by a particular molecular weight. These classes of fragments differ in molecular weight from the fragments produced by the action of the same enzyme on plaque-purified virus DNA. Three classes of fragments were present in less than equimolar amounts relative to the original DNA. The remaining six classes of fragments each contain more than one fragment per original DNA molecule. DNA-DNA hybridization analysis (using the filter method) of the isolated cleavage products demonstrated the presence of highly reiterated cell DNA sequences in two of the nine classes of fragments. A third class of fragments hybridized with high efficiency only to serially passaged SV40 DNA; the level of hybridization to plaque-purified virus DNA was low and there was essentially no hybridization with cell DNA immobilized on filters. It is suggested that this class of fragments contains unique host sequences. It was estimated that at least 27% of the sequences in the substituted SV40 DNA molecules studied are host sequences. The majority of these are probably of the nonreiterated type.


The community mental health professional and the poor.


Separation of DNA sequences complementary to the RNA of avian myeloblastosis virus from chicken DNA by alkaline cesium chloride density sedimentation. Density gradient sedimentation in alkaline cesium chloride of DNA from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13S revealed that the DNA sequences complementary to 70S avian myeloblastosis virus RNA sedimented in the high guanine plus cytosine region ahead of the main peak of cellular DNA. When the DNA was fragmented into pieces of 6.6S there was a broader distribution of the DNA sequences complementary to the viral RNA. This technique could be employed as a step towards the isolation of DNA copies of the entire viral RNA genome from the mass of host cellular DNA.


Bacterial resistance to antibiotics.

Bacterial resistance to antibiotics.


Speaking out: awash in freedom from hospitalization.


Cleavage of poliovirus-specific polypeptide aggregates. Zonal electrophoresis resolves two aggregates of poliovirus type 2 cytoplasmic polypeptides. The more negatively charged aggregate contains mainly noncapsid viral-specific polypeptides (NCVP) 2 and x, whereas the other consists of the capsid polypeptides (VP) 0, 1, 2, and 3 (VP0, VP1, VP2, VP3). After treatment with sodium deoxycholate (DOC), the aggregates sediment at 5 to 6S. Their electrophoretic mobilities are unaffected by DOC or RNase. The capsid polypeptide aggregate is similar in mobility to virions but can be converted to a faster electrophoretic form, resembling empty capsids, by heating. If infected HeLa cells are allowed to synthesize poliovirus polypeptides in the presence of iodoacetamide, no capsid polypeptides are produced, but rather NCVP1a (the precursor to capsid polypeptides) is accumulated, along with NCVP2 and NCVPx. When analyzed by electrophoresis and centrifugation, uncleaved NCVP1a migrates with the NCVP2-x aggregate. NCVP1a can be cleaved to capsid-like polypeptides in vitro by using extracts of infected cells, but not uninfected cells, indicating either a virus-specified protease or a cellular enzyme activated during infection. After cleavage of NCVP1a by infected cell extracts, the capsid polypeptides which are produced dissociate from the NCVP2-x complex.


Phosphorylation of animal virus proteins by a virion protein kinase. Compared with several other enveloped viruses, purified virions of frog virus 3 contained a relatively high activity of a protein kinase which catalyzed the phosphorylation of endogenous polypeptides or added substrate proteins. Virions also contained a phosphoprotein phosphatase activity which released phosphate covalently linked to proteins. It was possible to select reaction conditions where turnover of protein phosphoesters was minimal, as the phosphatase required Mn(2+) ions for activity whereas the protein kinase was active in the presence of Mg(2+) ions. Electrophoretic studies in polyacrylamide gels containing sodium dodecyl sulfate indicated that at least 10 of the virion polypeptides were phosphorylated in the in vitro protein kinase reaction. Characterization of these phosphoproteins demonstrated that the phosphate was incorporated predominantly in a phosphoester linkage with serine residues. The protein kinase was solubilized by disrupting purified virions with a nonionic detergent in a high-ionic-strength buffer and was separated from many of the virion substrate proteins by zonal centrifugation in glycerol gradients. The partially purified protein kinase would phosphorylate polypeptides of many different animal viruses, and maximal activity was not dependent on added cyclic nucleotides. These properties distinguished the virion protein kinase from a well characterized cyclic AMP-dependent protein kinase which phosphorylated viral proteins only to a small extent.


A new dental, ocular and cutaneous syndrome.


RNA synthesis in temperature-sensitive mutants of vesicular stomatitis virus. T-particle-free stocks of temperature-sensitive mutants representing the four Glasgow complementation groups of the Indiana serotype of vesicular stomatitis virus were used to study RNA synthesis at the permissive and nonpermissive temperatures of 31 and 39 C, respectively. Mutants selected from the four Glasgow complementation groups were characterized on the basis of particle and ribonucleoprotein formation. Intracellular RNAs were further characterized by polyacrylamide gel electrophoresis. ts G22 (group II) and ts G41 (group IV), previously characterized as RNA negative at the nonpermissive temperature, synthesized low levels of RNA which could not be attributed to contaminating levels of revertants. Furthermore, the levels of synthesis could not be reduced by the addition of cycloheximide. These data suggest that ts G22 (group II) and ts G41 (group IV) contain a thermally stable, virion-encapsidated transcriptase, but fail to amplify RNA synthesis due to a thermally labile function presumably necessary for the synthesis of viral RNA. ts G31, a group III mutant, synthesized intracellular RNA at amplified levels at the nonpermissive temperature. Intracellular ribonucleoprotein complexes were isolated in copious amounts; however, no particles corresponding in size to finished virions were observed. These data suggest a thermally labile maturation factor or envelope associated structural protein to be defective in ts G31 (group III). ts G11 (group 1) showed no detectable RNA synthesis at the nonpermissive temperature. These data suggest ts G11 (group I) contains a thermally labile component involved in early transcription. This group may contain a number of mutants defective in different components of the transcription apparatus, which may not complement in vivo because of the physical improbability of subunit exchange between virion particles of the incoming inoculum.


Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species. Class a and class b 30 to 40S RNA subunits obtained by heat dissociation from the 60 to 70S RNA complex of avian tumor viruses were compared with several RNA standards by electrophoresis in formamide-polyacrylamide gels. Class a RNA was found to have a lower electrophoretic mobility and hence probably a higher molecular weight than class b RNA. The absolute molecular weight of class a and b RNA could not be determined with accuracy, because the relationship between logarithm of molecular weight and mobility of the RNA standards was not linear. The size of class a RNA fell into the range of 2.4 x 10(6) to 3.4 x 10(6) daltons and that of class b into the range of 2.2 x 10(6) to 2.9 x 10(6) daltons, depending on the standards used. The possible biological significance of this difference is discussed.


Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. II. Behavior during incubation of isolated nuclei. The synthesis of polyoma DNA was studied in isolated nuclei from hydroxyurea-inhibited 3T6 cells infected with polyoma virus. During incubation of nuclei under conditions suitable for polyoma DNA synthesis in vitro, the short DNA fragments with a sedimentation coefficient of 4S formed in vivo (hydroxyurea fragments) became associated with preformed, replicating DNA strands. Centrifugation in dye-buoyant density gradients showed that the fragments formed part of the structure of the replicative intermediate of polyoma DNA. The proportion of "young" replicative intermediates was larger after hydroxyurea inhibition than in uninhibited controls. Hydroxyurea fragments appear to be closely related to the 4S fragments formed as normal intermediates during discontinuous synthesis of polyoma DNA.


Envelope of mouse mammary tumor virus studied by freeze-etching and freeze-fracture techniques. As part of a study of the cell surface changes associated with the production of murine mammary tumor virus, the structure of the envelope of this virus has been examined by using freeze-fracture techniques. Both fracture and deep-etch surfaces were examined. The fracture faces contain 10-nm spheres comparable to those observed on fractured plasma membranes, although fewer in number. Surfaces exposed by etching possess a highly regular hexagonal array of pits 25 nm apart. By examining freeze-fracture and freeze-etch preparations of virus with ferritin covalently bound to its surface, it has been determined that the surface exposed by etching is the outer surface of the virus. The pitted exterior surface of the mammary tumor virus appears to be a unique surface structure.


Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments. Hydroxyurea treatment of 3T6 mouse fibroblast cells infected with polyoma virus resulted within 15 min in more than a 20-fold reduction of the rate of both viral and cellular DNA synthesis. After the initial rapid inhibition, the rate of DNA synthesis remained essentially constant for at least 2 h. In the inhibited cells viral DNA accumulated as short chains with a sedimentation coefficient of about 4S (hydroxyurea fragments). A variable proportion of these fragments was released from the template strands when the viral DNA was extracted by the Hirt procedure. Reannealing experiments demonstrated that hydroxyurea fragments were polyoma-specific and probably synthesized on both parental strands at the replication forks.


Human papovavirus, BK strain: biological studies including antigenic relationship to simian virus 40. Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasmic vacuolization. The hemagglutinin of BKV is associated with the virion and is resistant to ether or heating at 56 C for 30 min. Fluorescent antibody as well as neutralization tests indicated antigenic similarities between simian virus 40 (SV40) and BKV. Cells undergoing lytic infection with BKV synthesized intranuclear T antigen(s) which reacted with SV40 T antibody demonstrable by immunofluorescence. However, BKV did not appear to induce SV40 transplantation antigens in transplantation-resistance tests. Evidence was obtained that BKV was present in humans prior to the widespread use of polio vaccines, thus ruling out the possibility that BKV is an SV40-related monkey virus, introduced into the human population by accidental contamination of poliovirus vaccines.


Use of nondefective adenovirus-simian virus 40 hybrids for mapping the simian virus 40 genome. A series of viable recombinants between adenovirus 2 (Ad2) and simian virus 40 (SV40) (nondefective Ad2-SV40 hybrids) have been isolated. The members of this series (designated Ad2(+)ND(1) through Ad2(+)ND(5)) differ from one another in the early SV40-specific antigens and the SV40-specific RNA species which they induce in infected cells. They also contain different amounts of SV40 DNA as shown by RNA-DNA hybridization techniques. We have examined the structure of the DNA molecules from these hybrids, using electron microscope heteroduplex mapping techniques. Each hybrid was found to contain a single segment of SV40 DNA of characteristic size covalently inserted at a unique location in the adenovirus 2 DNA molecule. The SV40 segments of the various hybrids formed an overlapping series with a common end point. When the results of the electron microscopic study were combined with data on antigen induction, it was found that a self-consistent map could be constructed which related specific regions of the SV40 genome to the induction of specific antigens. The order of these early SV40 antigen inducing regions in the SV40 DNA segments contained in the nondefective hybrids is: U antigen, tumor specific transplantation antigen, and T antigen with the U antigen region being nearest the common end point.


Mapping of simian virus 40 early functions on the viral chromosome. The simian virus 40 (SV40) DNA segment in the nondefective adenovirus 2-SV40 hybrid, Ad2(+)ND(4), is colinear with the segment between 0.11 and 0.59 SV40 fractional length from the site at which the R(1) restriction endonuclease cleaves SV40 DNA. This specifies the region of the SV40 DNA molecule which induces the early SV40 antigens: U antigen, tumor specific transplantation antigen, and T antigen. A variant of Ad2(+)ND(4), called Ad2(+)ND(4del), was found which has a deletion of the DNA segment between 0.50 and 0.57 SV40 fractional length from the R(1) endonuclease cleavage point.


Growth of pseudotypes of vesicular stomatitis virus with N-tropic murine leukemia virus coats in cells resistant to N-tropic viruses. Formation of pseudotypes between murine RNA tumor viruses and vesicular stomatitis virus (VSV) has been confirmed. Pseudotypes of VSV genomes coated by the surface envelope from an N-tropic tumor virus grew equally well in cells homozygous for either the Fv-1(n) or Fv-1(b) alleles. Therefore, the product of the Fv-1 locus, which restricts growth of murine RNA tumor viruses, must act on an intracellular aspect of tumor virus replication, a step after attachment and penetration.


Improved technique for the isolation of temperature-sensitive mutants of foot-and-mouth disease virus. An improved temperature-shift selection and screening method is described for the isolation of temperature-sensitive mutants of foot and mouth disease virus.


Proposed nomenclature for mutants of adenoviruses. In accord with the nomenclature proposed for mutants of simian virus 40 the same rules, with minor modifications, are recommended for naming mutants of adenoviruses. It is further suggested that these rules, which pertain to a system of classification based primarily upon complementation analysis, also be applied to mutants of other DNA-containing animal viruses.


A rapid and reproducible homogenization procedure for the isolation of plasma membranes from rat liver.


An inexpensive apparatus facilitating the storage of oxygen sensitive compounds.


Preparative electrophoresis of living mammalian cells in a stationary ficoll gradient.


Chlorphentermine-induced ultrastructural changes in liver tissues of four animal species.


Vinblastine-induced paracrystals and unusually large microtubules (macrotubules) in rat renal cells.


Intercellular contact in the unincubated chick embryo.


Fine structure of the developing spore of Nosema apis Zander.


Fluorescence histochemical and ultrastructural observations on the effects of intravenous injections of vinblastine on noradrenergic nerves.


Structure and innervation of the pineal gland of the rabbit, Oryctolagus cuniculus (L.). II. An electron microscopic investigation of the pinealocytes.


Effects of parathyroid hormone on osteoclasts in vivo. An ultrastructural and histochemical study.


Morphologic observations of brain tumors in PD4 hamsters induced by four strains of avian sarcoma virus.


Histological and ultrastructural observations on wound healing in the freshwater pulmonate Lymnaea stagnalis.


Pseudocroup with stridor.

Pseudocroup with stridor.


Ultrastructural study of the human anterior pituitary. Preliminary results.


Spontaneous and light-induced compound action potentials in the isolated eye of Aplysia: initiation and synchronization.


Effects of mammalian corticotrophin on the ultrastructure of presumptive interrenal cells in the opisthonephros of the lamprey, Petromyzon marinus L.


Dose effects of cortisol on single unit activity in hypothalamus, reticular formation and hippocampus of freely behaving rats correlated with plasma steroid levels.


Microelectrophoretic studies on the depressant action of HA-966 on chemically and synaptically excited neurones in the cat cerebral cortex and cuneate nucleus.


Equivalence of synaptic and injected current in determining the membrane potential trajectory during motoneuron rhythmic firing.


Effects of prostaglandins E1, E2 and F2a on cyclic AMP levels in brain in vivo.


Masseteric reflex response to reticular stimulation reverses during active sleep compared with wakefulness or quiet sleep.


High affinity uptake of cyclic AMP in rat brain slices.


Electron paramagnetic resonance (34 and 55 GHz) studies of hemoproteins.


Synthesis of glycerol-1,3-diphosphonic acid.


Nucleotide clusters in deoxyribonucleic acids. 8. The pyrimidine oligonucleotides of bacteriophage S13 wild type DNA.


Amebiasis: a ten-year review of clinical and epidemiological progress in the Saskatchewan endemic area.


Serum phospholipids in children with familial hypercholesterolaemia.


A role for ferritin in the regulation of iron metabolism.


Pseudomonas bacterial endocarditis. A case report.


Dogfish lactate dehydrogenase. The stereochemistry of hydrogen transfer.


The presence of lactic acid in purified lactate dehydrogenase.


Inactivation of ADH from yeast and GAPDH from rabbit muscle by structural analogues of NAD.


A study on the cleavage site specificity of the endonuclease associated with purified simian virus 40 particles.


Studies on the metabolism of cyclic nucleotides by the toad bladder: the metabolic products of cAMP.


The production of cIMP by toad bladder, and its effects on transport of water and salt.


Butanol-soluble glycosyl transferases in Klebsiella aerogenes.


Cytochrome c (553, Chlorobium thiosulfatophilum) is a sulphide-cytochrome c reductase.


The influence of Mg2+-adenine nucleotide ratios and absolute concentration of Mg2+-adenine nucleotide on the observed velocity of some kinase reactions.


Influence of uncouplers on succinate-cytochrome c reductase.


Distribution of insulin receptor sites among liver plasma membrane subfractions.


The chemotactic effect of cyclic nucleotides with substitutions in the base ring.


Action of human skin collagenase on cartilage collagen.


Purification by affinity chromatography of thermostable glyceraldehyde 3-phosphate dehydrogenase from Thermus aquaticus.


Formation of guanosine tetraphosphate (magic spot I) in homologous and heterologous systems.


Depolarization induced calcium release from sarcoplasmic reticulum membrane fragments by changing ionic environment.


Does formylation of initiator tRNA act as a regulatory signal in E. coli?


Nutritional correlates of child development in Southern Tunisia. 3. Skeletal growth and maturation.


Collagenase activity in cutaneous scars.


On the mechanism of radioprotection by cysteamine. II. The significance of cyclic 3',5'-AMP for the cysteamine-induced radioprotective effects in white mice.


Letter: E.s.r. studies on gamma-irradiated TAN (triacetoneamine n-oxyl) radicals.


A rapid technique for obtaining one-step or multi-step antibiotic resistant mutants employing mutator strains of Escherichia coli K12.


Mitochondrial biogenesis during differentiation of Artemia salina cysts. Mitochondria isolated from cysts of Artemia salina (brine shrimp) were found to be devoid of cristae and to possess a low respiratory capability. Hydration of the cysts induces marked biochemical and morphological changes in the mitochondria. Their biogenesis proceeds in two stages. The first stage is completed within 1 h and is characterized by a rapid increase in the respiratory capability of the mitochondria, their cytochrome oxidase, cytochrome b, cytochrome c and perhaps some morphological changes. In the second stage there is an increase in the protein-synthesizing capacity of the mitochondria as well as striking changes in mitochondrial morphology leading to the formation of cristae.


Antivirals. 1. 2-(Alpha-hydroxybenzyl)imidazo(4,5-C)pyridine.


Iodination of estrogen receptors in rat uterine eosinophils.


Effect of ampicillin treatment on the urinary excretion of estriol conjugates in pregnancy.


Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. Denatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced the fraction of sonically treated, denatured adenovirus DNA bound to hydroxyapatite from about 30% to less than 1%. This led to a study of the effect of formamide on the melting temperature (T(m)) of double-stranded DNA in solution or bound to hydroxyapatite. The T(m) of DNA decreases 0.56 C/1% formamide, a value determined in buffered solutions with purified formamide.


Oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. The in vitro transformation of hamster embryo fibroblasts by herpes simplex virus type 1 (HSV-1) after exposure of the virus to UV irradiation is described. Cell transformation was induced by 2 out of 12 strains of HSV-1 that were tested for transforming potential. Cells transformed by the KOS strain of HSV-1 were not oncogenic when injected into newborn Syrian hamsters. However, cells transformed by HSV-1 strain 14-012 induced tumors in 47% of the newborn hamsters injected. HSV-specific antigens were found in the cytoplasm of cells transformed by both virus strains. Sera from tumor-bearing hamsters contained HSV-1- and HSV-2-neutralizing antibodies as well as antibodies which reacted specifically with HSV antigens by the indirect immunofluorescence technique. Hamster oncornavirus antigens were not detected by immunofluorescence methods. These observations represent the first evidence of the oncogenic potential of HSV-1.