Determination of bone age and recording of minor skeletal hand anomalies in normal children.


Susceptibility of herpesviruses to three nucleoside analogues and their combinations and enhancement of the antiviral effect of acid pH.


Ionic dependence of adrenal steroidogenesis and ACTH-induced changes in the membrane potential of adrenocortical cells. 1. The effects of changes of ionic environment upon corticosteroid production by rabbit adrenal glands have been investigated in vitro using a superfusion technique and on-line steroid analysis by an automated fluorescence method. In some experiments micro-electrode recordings of adrenocortical transmembrane potentials were made concomitantly with measurement of steroid output.2. Adrenocorticotrophic hormone (ACTH), 10 m-u./ml., induced a sevenfold increase in corticosteroid production rate in normal Krebs solution.3. The steroidogenic response to ACTH was not impaired after omission of [K](o) for 1 hr but was inhibited following exposure to K(+)-free medium for 3 hr. Increase of [K](o) tenfold to 47 mM increased the basal but not the ACTH-stimulated output of corticosteroid whereas raising [K](o) twentyfold to 94 mM enhanced both the basal and ACTH-stimulated steroid production rate. In K(+)-free solution the adrenocortical cells hyperpolarized from - 67 to - 86 mV; subsequently on addition of ACTH they depolarized. Reintroduction of K(+) restored the membrane potential.4. Omission of Ca(2+) partially depolarized the cells but only affected the steroidogenic response to ACTH in the presence of EDTA. A threefold increase of [Ca](o), to 7.68 mM, had no effect on either membrane potentials or steroid formation, but increasing [Ca](o) tenfold to 25.6 mM partially blocked ACTH action. Increasing [Mg](o) twentyfold to 22.6 mM had little effect on ACTH-stimulated corticosteroid output and Sr 2.56 mM, in substitution for Ca(2+), supported ACTH action, but La, 0.25 mM, completely blocked the steroidogenic effect of ACTH.5. Replacement of NaCl, 118 mM by choline chloride, 118 mM, was without effect on ACTH-induced steroidogenesis, whereas LiCl, 118 mM, reduced it by 50%. NaF, 1 and 10 mM, inhibited ACTH-induced steroidogenesis by approximately 60%.6. Nupercaine, 10(-4)M, inhibited the steroid response to ACTH with no effect upon membrane potentials: increasing the nupercaine concentration to 10(-3)M inhibited the steroid response and depolarized the cells. Ouabain, 10(-5)M, induced complete depolarization and suppression of the steroidogenic response to ACTH.7. Action-potential-like changes in membrane potential appeared in cells exposed to ACTH in a K(+)-free medium. The amplitude of the action potentials ranged from 10 to 60 mV according to cell, with a frequency up to 36/min; the frequency tended to increase with time. Tetrodotoxin, 10(-6) g/ml., did not inhibit ACTH-induced action potentials in K(+)-free medium.8. These observations are discussed in relation to the ionic requirements for the steroidogenic action of ACTH. The results further emphasize the dissociation of membrane polarization and the secretion of steroid. The mechanism of output of steroid hormone from the adrenocortical cell may thus differ fundamentally from the secretory mechanisms in other, particle-storing cells.


Relaxing action of synthetic antispasmodics on the intestinal smooth muscle of guinea pig.


Effects of cyclic 3',5'-AMP and related compounds on catecholamine-induced contraction of isolated guinea-pig vas deferens.


Metabolic pathways involved in the oxidation of isopropanol into acetone by the intact rat.


Possibilities for drug development based on the cyclic AMP system.


Hormonal control of bone cell function.

Hormonal control of bone cell function.


Physiological significance of the vitamin D-dependent calcium-binding protein.


Electron microscopic-immunocytochemical localization of adrenocorticotropin and melanocyte stimulating hormone in the pars intermedia cells of rats and mice.


Light and electron microscopy of the early response of Escherichia coli to a 6beta-amidinopenicillanic acid (FL 1060).


Microphotometry for determining the reaction rate of O2 and CO with red blood cells in the chorioallantoic capillary.


Disintegration of single cells in suspension. Isolation of rabbit polymorphonuclear leucocyte granules.


The nephelometric and membrane filtration technique applied to serum lipoprotein investigation.


Ultracentrifugation of human lipoproteins.


Biological significance of serum lipoproteins.


Origin and fate of lipoproteins.

Origin and fate of lipoproteins.


The metabolism of LDL.

The metabolism of LDL.


The intravenous fat tolerance test with intralipid in various types of hyperlipidaemias and comparison between metabolism of intralipid and VLDL.


The effects of catecholamines on isolated perfused coronary arteries in the dog.


The biogenesis and metabolism of steroids in the human placenta of 9--14 weeks in vitro.


A study of some bradykinin potentiating peptides derived from plasma proteins.


The pharmacology of dibenzofuran and terphenyl derivatives of DMAE and hemicholinium-3.


Macrophage activation in mice infected with ectromelia or lymphocytic choriomeningitis viruses.


Fine structure of phagocytic cells in the chiton, Liolophura gaimardi.


Replication of a cell-culture-modified infectious laryngotracheitis virus in experimentally infected chickens.


Protection of chickens against Marek's disease virus JM-V strain with statolon and exogenous interferon.


An avian adenovirus associated with respiratory disease.


Chronologic study of the T-virus in chicks. I. Development of lesions.


The use of silica gel for human calcitonin isolation in some thyroid diseases.


The effect of cyclazocine on the cardiovascular responses to pressor and depressor amines in the rat.


Serum and tissue dopamine-beta-hydroxylase activity in hypophysectomized rats.


Dictyostelium discoideum aggregation fields as excitable media.


Chronologic study of the T-virus in chicks. II. Development of hematologic changes.


Effect of social stress and steroids on antibody production.


The effect of hyperactivity on synaptic transmission. Experiments in Blatta orientalis.


Adenosine metabolism: properties of dog heart microsomal 5'-nucleotidase.


Viral isolation rates during summer from children with acute upper respiratory tract disease and healthy children.


Outbreak of a nosocomial infection with a strain of Proteus rettgeri resistant to many antimicrobials.


Recovery from severe cytomegalovirus pneumonia in a renal transplant patient.


Massive intrapulmonary venoarterial shunting in alveolar cell carcinoma. A case report.


Guidelines for work for patients with tuberculosis.


Effects of 2-N-(p-chlorobenzoyl)-N-(2-hydroxyethyl)aminomethyl-3-methyl-norbornane on ganglionic transmission and response to acetylcholine and pilocarpine.


Effects of essential phospholipids (EPL) on experimental cobalt-induced cardiomyopathy.


The synergistic action of antifertility compounds in the male rat.


Aflatoxin inhibition of rat liver mitochondria.


ACTH, glucagon and gastrin production by a pancreatic islet cell carcinoma and its treatment.


Interaction between secretion of the gonadotrophins, prolactin, growth hormone, thyrotrophin and corticosteroids in man: the effects of LH FSH-RH, TRH and hypoglycaemia alone and in combination.


An improved isolation method and new composition data for Forssman hapten from sheep erythrocytes.


Decreased vasoconstrictor response to angiotensin II in the presence of vascular stenosis.


The inhibition by ouabain of phospholipid turnover in the myocardium of the rabbit.


Protein fractions from mouse salivary glands affecting epithelial cells in organ culture. 1. Extracts of male mouse submaxillary gland have been fractionated on G 150 Sephadex and DEAE cellulose.2. A number of the protein fractions obtained appear to cause proliferation of epithelial cells in organ cultures of rat tissues.3. All fractions active in organ culture possess proteolytic enzyme activity.4. The proteolytic enzymes trypsin, pronase and collagenase produce epithelial disorganization in organ culture.5. The organ culture effect is reduced in the presence of proteolytic enzyme inhibitors and abolished by pretreatment of proteins with diisopropylfluorophosphonate.6. The presence of mitotic inhibitors does not reduce the organ culture effect.7. The effect is not associated with an increase in the DNA content of tissue explants.8. No evidence has been found for the existence, in mouse salivary glands, of a specific growth factor acting on epithelial cells.


Proceedings: Excitatory and inhibitory monosynaptic actions mediated by a serotonin containing neurone in Aplysia californica.


The effect of repetitive stimulation on facilitation of transmitter release at the frog neuromuscular junction. 1. End-plate potentials (e.p.p.s) were recorded from frog neuromuscular junctions blocked with high Mg and/or low Ca to characterize the processes underlying increased transmitter release during repetitive stimulation.2. There was a progressive increase in the amplitude of successive e.p.p.s during repetitive stimulation. Increasing the frequency or duration of stimulation increased this facilitation of e.p.p. amplitudes. Facilitation is defined as the fractional increase in amplitude of a test e.p.p. over a control.3. By assuming that each impulse in a train contributes an identical increment of facilitation that sums linearly with the facilitation contributed by the previous impulses, estimates of the facilitation contributed by a single impulse, f(t), were made from the incremental increase in e.p.p. amplitudes during repetitive stimulation. The average value of f(t) contributed by the first impulse in the train during stimulation at 20/sec is given by f(t) = 0.8 e(-t/50) + 0.12 e (-t/300) + 0.025 e(-t/3000),where t is in msec. The first two terms in this equation were independent of the stimulation rate used to determine f(t) while the coefficient of the third term was a function of the stimulation rate, decreasing 2 to 3 times when the stimulation rate was decreased from 20/sec to 1/sec.4. This linear facilitation model predicted growth of e.p.p. amplitudes during the first several hundred msec of repetitive stimulation. Thereafter, e.p.p. amplitudes were typically facilitated more than predicted by the linear model.5. Several new methods are presented which can be used to obtain estimates of the magnitude and time course of facilitation contributed by specific impulses during repetitive stimulation.6. It is found that the value of short-term f(t) in the tested range of 25-300 msec progressively increases during repetitive stimulation while its time course of decay remains unchanged. After 9 sec of stimulation at 20/sec, the short-term f(t) increased to 1.4 times control.7. The increase in short-term f(t) was independent of whether it was determined from a step increase or decrease in total facilitation, excluding the possibility that the observed increase in short-term f(t) resulted from a change in the rate of decay of facilitation.8. It is suggested with supporting data from the following paper (Magleby, 1973) that each impulse contributes two types of facilitation that are responsible for the growth of e.p.p.s during repetitive stimulation: a short-term facilitation with linear summation properties described by the first two terms in the expression in paragraph 3 and a long-term cumulative facilitation approximated by the third term. The long-term facilitation is expressed as an increase in both the short-term facilitation and in the base level of transmitter release. The relative contribution of these two expressions of the long-term facilitation to the third term is a function of the stimulation rate and is given by the ratio of facilitation to the base level of transmitter release.


Proceedings: Ultrastructural changes at the synapse associated with depression of synaptic transmission in rat superior cervical ganglion after injury of post-ganglionic axons.


The effect of tetanic and post-tetanic potentiation on facilitation of transmitter release at the frog neuromuscular junction. 1. End-plate potentials (e.p.p.s) were recorded from frog neuromuscular junctions blocked with high Mg and/or low Ca.2. Estimates of f(t), the facilitation contributed by each impulse, were obtained during and following repetitive stimulation from the incremental change in e.p.p. amplitudes following step changes in the stimulation rate during the conditioning and testing trains.3. Estimates of f(t) increased during the conditioning stimulation and returned to control in the post-tetanic period. This increase in f(t) was proportional to the magnitude of tetanic or post-tetanic potentiation (PTP) present.4. These results are described by: [Formula: see text] where h(t)/h(c) is the e.p.p. amplitude at time t expressed in terms of the control, P(t) is potentiation, F(t) is facilitation and 1 is the base level of transmitter release. Thus, potentiation has a multiplicative (gain) effect on facilitation and the base level of transmitter release.5. PTP was present immediately following the conditioning train. However, if depression occurred during the conditioning train, PTP developed after a delay.6. It is suggested that facilitation and potentiation represent increases in two independent factors which act jointly to increase the probability of transmitter release.


Proceedings: Increased drinking in dogs during congestive heart failure.


Proceedings: The upper limit of 'autoregulation' of cerebral blood flow in the baboon.


Proceedings: Periodicities in ventilation in new-born infants.


Proceedings: Presynaptic inhibition and the depressant actions of GABA and glycine in the feline cuneate nucleus: changes related to electrographic seizure activity.


Proceedings: Renin and angiotensin II of extrarenal origin in the plasma of female rabbits.


Optical diffraction on tilting series of paracrystalline intranuclear inclusions of dog liver parenchymal cells.


Proceedings: Prostaglandin E1 synthesis by phagocytosing rabbit polymorphonuclear leucocytes: its inhibition by indomethacin and its role in chemotaxis.


Fine structure of spermiogenesis in scorpions from the family Vejovidae.


Diagnosis of glycogen storage disease type I by serum immunodiffusion.


Carcinogenicity of inhaled plutonium-238 in the rat.


Functional interpretation of lymphography according to contrast medium persistence in the lymph nodes.


Organization of glycolysis: oscillatory and stationary control.


Kinetics of the renin system. The basis for determination of the different components of the system.


Regulation of gene expression by cyclic AMP in Escherichia coli.


Mitochondrial-cytosolic interactions in cardiac tissue: role of the malate-aspartate cycle in the removal of glycolytic NADH from the cytosol.


Rate control by insulin and its mechanism.


Enzyme conformation and control.

Enzyme conformation and control.


Frequency of renal intraendothelial microtubular inclusions in kidney transplants. An electron microscopy study.


Search for BK and SV40 virus reactivation in renal transplant recipients.


Studies on the tumor target cell destruction by in vitro sensitized lymphocytes.


Ultrastructure of experimental brain gliomas in mice.


Immunobiology of genitourinary tumors.

Immunobiology of genitourinary tumors.


The cytopyge of Ciliata. II. Microphotographical documentation of the defecation in Prorodon teres.


A comparative study on the seed proteins of induced mutants of the pea.


The cytopyge of Ciliata. 3. Microphotographical documentation of the defecation in Blepharisma ovata.


Increased cellular reaction to damage caused by angiotensin in arterioles of normal recipient rats after transfer of lymphocytes from hypertensive rats.


The effect of cyclic AMP on the erythropoiesis in mice(NMRI).


Cause of the prolonged pressor action of renin in nephrectomized rats. Elucidated by means of anti-angiotensin II and of angiotensin-inhibitor (1-sar-8-ala-angiotensin II).


Role of the renin-system in normo- and hypertension. Effect of angiotensin-inhibitor (1-sar-8-ala-angiotensin II) on the blood pressure of conscious or anaesthetized normal, nephrectomized and renal hypertensive rats.


On the side-effects of contrast media for myelography. A histological examination of the spinal cord, nerve roots and meninges after experimental myelography with iodophendylate and methiodal.


Rapid marked increase in plasma renin in rats treated with inhibitors of the renin system. Effects of 1-sar-8-ala-angiotensin II and of a synthetic converting enzyme inhibitor (nonapeptide, SQ 20.881) on normal and adrenalectomized rats.


Experiences with depot neuroleptics in an after-care department.


Protein deprivation in primates. I. Nonpregnant adult rhesus monkeys.


Electron microscopic observations on human glioblastomas and astrocytomas maintained in organ culture systems. The fine structure of four glioblastomas and two cerebellar astrocytomas maintained in organ culture systems up to 137 days and 43 days, respectively, using either a three-dimensional sponge foam matrix technic or a Millipore filter platform technic, is described and compared. The cells of both tumor types showed increased astrocytic differentiation, characterized by a progressive increase in glial filaments associated with an increase in free ribosomes and granular endoplasmic reticulum. A progressive increase in basement membrane material, presumably originating from explanted endothelial cells or pericytes, was also found in both tumor types and was often associated with increased numbers of collagen fibrils. Astrocytic tumor cell processes frequently preserved their contact with this basement membrane material. Microvascular fenestrations or gaps in endothelial cells were not identified. These electron microscopic features appear to correspond to the early stages of perivascular sclerosis previously noted by light microscopy in gliomas maintained in organ culture systems and are presumably related to the progressive obliteration of the functional microvasculature.


Salmonella typhimurium hisG46 (R-Utrecht): possible use in screening mutagens and carcinogens. Salmonella typhimurium LT2 hisG46 becomes a more sensitive strain for assaying mutagens and carcinogens when it carries the resistance transfer factor R-Utrecht.


Lipid metabolism and experimental atherosclerosis in baboons: influence of cholesterol-free, semi-synthetic diets.


Spiradenoma. Histochemical and electron microscopic study.


Vitamin E treatment of epidermolysis bullosa. Changes in tissue collagenase levels.


Letter: Intertriginous xanthomas.

Letter: Intertriginous xanthomas.


A difference in the breakdown of phosphatidylinositol in normal and SV40 transformed mouse fibroblasts.


Separation and comparison of primary structures of three formylmethionine tRNAs from E. coli K-12 MO.


The valence of copper and the role of superoxide in the D-galactose oxidase catalyzed reaction.


Electron paramagnetic resonance studies of iron-sulfur centers in mitochondria prepared from three Morris hepatomas with different growth rates.


The effects of some cations and anions on spin labeled cytoplasmic membranes of Bacillus subtilis.


Cyclic changes in levels of cyclic AMP and cyclic GMP in frog myocardium during the cardiac cycle.


Altered nuclear RNA transport associated with carcinogen intoxication in rats.


Adrenocorticotropic hormone and dibutryl adenosine cyclic monophosphate-mediated Ca2+ uptake by rat adrenal glands.


The effect of glucose ingestion on basal and induced enzyme levels in rat tissues.


The delta-aminolevulinic acid synthetase activity and the effect of exogenous delta-aminolevulinate on the synthesis of cytochrome c in the thoracic muscles of the tobacco horn worm during adult development.


A bromine compound isolated from human cerebrospinal fluid.


Variation of proteins, enzyme markers and gangliosides in myelin subfractions.


Effects of infusions of catecholamines, angiotensin, vasopressin and histamine on hepatic blood volume in the anaesthetized cat. 1. Hepatic volume was recorded by a plethysmographic technique in cats anaesthetized with pentobarbitone; the hepatic artery and portal vein remained intact. Dose-response curves were obtained for intravenous infusions of adrenaline, noradrenaline, angiotensin, vasopressin and histamine.2. Adrenaline and noradrenaline decreased hepatic blood volume and did not differ significantly in potency. Up to 40% of the hepatic blood volume was expelled by doses within the range secreted by the adrenal medullae.3. Isoprenaline, infused into the hepatic artery, had no significant effect on hepatic blood volume in doses which caused maximal vasodilatation of the hepatic arterial bed. Relaxation of hepatic capacitance vessels mediated by beta-adrenoceptors did not occur.4. Angiotensin infusions in doses previously shown to cause intestinal and splenic vasoconstriction, decreased hepatic blood volume and on a molar or microgramme basis, angiotensin was the most potent of the agents tested. Doses within the probable physiological range of endogenous production decreased hepatic blood volume by up to 20%. The responses were not significantly different when the hepatic nerves were intact or sectioned.5. Vasopressin infusions produced only small decreases in hepatic blood volume. Doses within the range secreted by the posterior pituitary which constrict the intestinal and splenic resistance vessels, did not decrease hepatic blood volume by more than 10%.6. Histamine produced no change in hepatic blood volume in doses which readily produce outflow block in dogs. Either the specific hepatic venous smooth muscle involved in outflow block is absent in the cat or it has no histamine receptors.7. After the rapid change in hepatic blood volume at the onset of the infusion, hepatic volume remained steady for the duration of each infusion. There was no evidence that these agents caused net transsinusoidal fluid movements.


Some aspects of the inhibition of the action of antidiuretic hormone by lithium ions in the rat kidney and bladder of the toad Bufo marinus. 1. The effect of intravenous infusions of various ions on the antidiuretic action of antidiuretic hormone has been studied in rats.2. Lithium (13 mmol/l.) reversibly inhibits the antidiuretic responses. Similar concentrations of potassium, rubidium, strontium, magnesium, choline and calcium do not. Lithium has a similar effect on the antidiuretic activity of oxytocin.3. The inhibition is not simply related to blood nor whole body lithium concentrations.4. Lithium (2 mmol/l.) in contact with the serosal surface also inhibits the transport of water facilitated by either 0.5 U/l. antidiuretic hormone or 1.1 mmol/l. cyclic adenosine monophosphate in the isolated toad bladder.5. Choline (2 mmol/l.) on the serosal surface also inhibits the transport of water facilitated by vasopressin in the toad bladder.


Selective inhibition of angiotensin pressor responses in the pithed rat by tetraethylthiuram disulphide (disulfiram) and sodium diethyldithiocarbamate (DDC). 1. Pressor responses to sympathetic outflow stimulation, noradrenaline and angiotensin have been recorded in pithed rats.2. Disulfiram (50 mg/kg) and sodium diethyldithiocarbamate (DDC) (5-100 mg/kg) both caused an initial increase in the pressor response to all three procedures followed by a selective inhibition of the angiotensin responses.3. Penicillamine (1-100 mg/kg) and ascorbic acid (1-500 mg/kg) increased the pressor responses to all three procedures without any subsequent blocking action.4. Reserpine (5 mg/kg daily for 3 days) abolished responses to sympathetic outflow stimulation but did not impair angiotensin or noradrenaline responses.5. In reserpinized rats, the initial enhancement of angiotensin responses after disulfiram and sodium diethyldithiocarbamate was absent or reduced and the onset of the subsequent block was accelerated.6. Possible mechanisms for the angiotensin-blocking action of disulfiram and sodium diethyldithiocarbamate are discussed.


Proceedings: Alterations in the sleep-wakefulness cycle in rats after administration of (-)-LSD or BOL-148: a comparison with (+)-LSD.


Proceedings: Active factors in the venom duct of Conus californicus.


Proceedings: Concentrations of the oxime 2-hydroxyiminomethyl pyridinium methyl methane sulphonate (P2S) after intramuscular injections in humans.


Proceedings: New dialysis technique for the continuous measurement of the concentration of vasoactive hormones.


Proceedings: Tissue cyanide concentrations and cytochrome oxidase in experimental cyanide poisoning.


The modern ankle-foot orthoses (AFO's).

The modern ankle-foot orthoses (AFO's).


Human pancreatic islet tumor cells maintained in vitro.


Inhibition of glucose-induced insulin secretion in trypsin-treated islets of Langerhans.


Effect of diabetes and insulin on the rat heart adenyl cyclase, cyclic AMP phosphodiesterase and cyclic AMP.


Plasma ACTH in the adrenalectomized rat.


Serum parathyroid hormone concentrations in vitamin D deficiency rickets of infancy: effects of intravenous calcium and vitamin D.


Melanotic progonoma of the brain.

Melanotic progonoma of the brain.


The histiocytic infiltrates of the skin.


Postnatal cytomegalovirus hepatitis. An autopsy and liver biopsy study.


Inhibition of cell growth in the G1 phase by adenosine 3', 5'-cyclic monophosphate. Incorporation of tritiated thymidine into acid-precipitable material was used to measure the rate of DNA synthesis in secondary cultures of human diploid fibroblasts. Confluent cultures of human diploid fibroblasts, which are synchronized in the G(1) phase due to contact inhibition, were released from growth inhibition either by the addition of fresh medium to the cultures or by trypsinization and replating at nonconfluent densities. Either treatment resulted in a synchronous wave of DNA synthesis beginning 10-15 h after treatment and peaking at 20-25 h. In confluent cultures stimulated by fresh medium, either the addition of 0.25 mM N(6), O(2)-dibutyryl-adenosine 3',5'-cyclic monophosphate (db-cAMP) to the medium in the interval 4-8 h after stimulation or the replacement of the fresh medium in that same 4 h interval with the depleted medium present on the cells for the 2 day period before stimulation delayed the synchronous onset of DNA synthesis in the cultures by about 4 h. In nonconfluent cultures freshly seeded from trypsinized confluent cultures, this same depleted medium obtained after a 2 day incubation of fresh medium on confluent cultures is shown to support the progress of the cells into S phase; however, the addition of 0.25 mM db-cAMP to the medium 3(1/2) h after replating still partially prevented the initiation of DNA synthesis in the cultures. The results are discussed in terms of the role of serum and cAMP in the control of cell growth in fibroblast cultures.


Editorial: Role of the kidney in the metabolism of calciferol (vitamin D).


Cytochemistry of Golgi fractions prepared from rat liver. Cytochemical tests for several marker enzymes were applied to liver tissue and to the three Golgi fractions (GF(1), GF(2), GF(3)) separated by the procedure of Ehrenreich et al. from liver homogenates of alcohol-treated rats. 5'-Nucleotidase (AMPase) reaction product was found in all three fractions but in different locations: It occurred along the inside of the membrane of VLDL-filled vacuoles in GF(1) and GF(2), and along the outside of the cisternal membranes in GF(3). In the latter it was restricted to the dilated cisternal rims and was absent from the cisternal centers. The AMPase activity found in the fractions by biochemical assay is therefore indigenous to Golgi components and is not due to contamination by plasma membrane. Acid phosphatase (AcPase) reaction product was detected within lysosomal contaminants in GF(1) and within many VLDL-filled vacuoles in GF(1) and GF(2), indicating that AcPase activity is due not only to contaminating lysosomes, but also to enzyme indigenous to Golgi secretory vacuoles. G-6-Pase reaction product was present in GF(3) and within contaminating endoplasmic reticulum fragments, but not in other fractions. Thiamine pyrophosphatase (TPPase) was localized to some of the VLDL-filled vacuoles and cisternae in GF(1) and GF(2), and was not found in the cisternae in GF(3). The results demonstrate the usefulness of cytochemical methods in monitoring the fractionation procedure: They have (a) allowed a reliable identification of contaminants, (b) made possible a distinction between indigenous and contaminating activities, and (c) shown, primarily by the results of the TPPase test, that the procedure achieves a meaningful subfractionation of Golgi elements, with GF(1) and GF(3), representing primarily trans-Golgi elements from the secretory Golgi face, and GF(3) consisting largely of cis-Golgi components from the opposite face.


Adrenocorticotropin. 45. Synthesis and steroidogenic activity of (1-alanine)adrenocorticotropin-(1-20)-amide, (1-D-alanine)adrenocorticotropin-(1-20)-amide, and (1-14C)alanine)adrenocorticotropin-(1-20)-amide.


Angiotensin-like and antagonistic activities of N-terminal modified (8-leucine)angiotensin II peptides.


Synthesis and anti-deoxyribonucleic acid virus activity of certain 9-beta-D-arabinofuranosyl-2-substituted adenine derivatives.


Propagation of both parental mitochondrial DNAs in rat-human and mouse-human hybrid cells.


Angiotensin analogs: the influence of sarcosine substituted in position 1.


Inhibition of adrenergic vasoconstriction by quinidine.


Renin release and ketamine-induced cardiovascular stimulation in the rat.


Effect of certain halogenated hydrocarbon insecticides on cyclic adenosine 3',5'-monophosphate-3H formation by rat kidney cortex.


Lipid-protein and lipid-lipid interactions in cytochrome oxidase model membranes.


The fluid state of lecithin bilayers.

The fluid state of lecithin bilayers.


Synaptic vesicles: structure of chromaffin granule membranes.


Rhodopsin-lipid interactions.

Rhodopsin-lipid interactions.


Adenylate cyclase and cyclic AMP phosphodiesterase of normal and Rous sarcoma virus transformed chicken embryo fibroblasts.


The effect of cyclic AMP on membrane permeability.


Red cell aggregation by macromolecules: roles of surface adsorption and electrostatic repulsion.


New developments in pharmacology of vitamin D.


Host defenses against viral disease.

Host defenses against viral disease.


Inapparent congenital cytomegalovirus infection with elevated cord IgM levels. Casual relation with auditory and mental deficiency.


The clinical value of the magnification technique in cerebral angiography.


Editorial: Subtle effects of perinatal infection.


Vincristine therapy in malignant glioma recurrencies.


Increased activity of adenine phosphoribosyltransferase in erythrocytes of normal newborn infants.


Antibodies to Epstein-Barr virus at the ages of 6 to 23 months in children with congenital heart disease.


Ethanol consumption and enzyme activities in Sinclair miniature pigs.


Marital group therapy in the treatment of alcoholism.


Serologic studies of infections in patients with hematologic malignancy.


Inhibition by castration of aflatoxin-induced hepatoma in carbon tetrachloride-treated rats.


Serotypes and antibiotic susceptibility of Escherichia coli in non-enteric infections of children.


The cardiotoxic effects of pressurized aerosol isoproterenol in the dog.


The bone cell system: form and function.


Hepatoma--nature's model tumor. A review.


Tumor doubling time and survival of men with bronchogenic carcinoma.


Structural requirements for substrates and inhibitors of angiotensin I-converting enzyme in vivo and vitro.


Fulminant lymphoma mimicking pneumocystis carinii pneumonia.


Sensitization of the adrenal cortex to angiotensin II in sodium-deplete man.


The pathophysiology of renin.

The pathophysiology of renin.


Hexachlorophene revisited.

Hexachlorophene revisited.


Effects of neuroleptanesthesia on endocrine function in man.


The surgical management of axillary hyperhidrosis.


Sophisticated simplification for medical services.


Helen Brenton Pryor, M.D.--pediatrician. (1898-1972).


Letter: Women to the rescue.

Letter: Women to the rescue.


History of the library trust fund.

History of the library trust fund.


A study of hepatitis-associated antigen in hospital patients.


Tumor rejection and immunotherapy studies with simian virus 40-induced tumors.


Pentose shunt enzyme activities in non-inflamed attached gingiva of dogs.


The lung--an endocrine organ?

The lung--an endocrine organ?


Diabetic foot.

Diabetic foot.


Persistent cloaca with no urethral, genital, or anal openings.


Perinatal infections and epidemiology of anencephaly and spina bifida.


Skin hypersensitivity to parainfluenza-3 virus in cattle.


The aetiology of perceptive deafness. A clinico-pathological and anatomical study of temporal bones and brain structures.


Making referrals is everybody's business.


Dynamic response of the intact canine adrenal to infused ACTH.


Systems analysis of arterial pressure regulation and hypertension.


Influence of binding on the pharmacologic activity of antibiotics.


The utilization of fluorescent microscopy in routine chromosome analysis.


Contraceptive usage among teenagers.

Contraceptive usage among teenagers.


Suicide in children and adolescents.

Suicide in children and adolescents.


Nepotism: paradoxical liberation.

Nepotism: paradoxical liberation.


The management of emotional crises by medical practitioners.


Metatarsalgic neurogenica. A definitive clinical syndrome.


The quantity and estrogenic activity of F-2 toxin produced on different substrates.


Experimental study on combined therapy with anticancer drugs and urokinase.


Parotid benign giant mixed tumor of forty-two years duration with malignant transformation.


Possible aetiological factors of calciphylaxis in pigs.


Hepatoma in a mangabey (Cercocebus atys).


Pneumoconiosis in the captive New Zealand kiwi.


Neonatal calf diarrhea. Electron microscopy of intestines infected with a reovirus-like agent.


Breast carcinoma with late recurrence (cytological type 3).


Epstein-Barr virus infection in the neonatal period and in childhood. In an attempt to detect and characterize congenital, neonatal and early childhood EBV infections, a prospective sero-epidemiological study was undertaken in 112 newborn infants and their mothers, 25 additional newborns undergoing exchange transfusion, 114 randomly selected hospitalized infants aged 0 to 3 years, and 109 siblings and parents of these infants. Leukocyte culture was attempted in all the newborns and in 25 pre- and post-transfusion.The findings of EBV seroconversion in six patients without clearly apparent illness, infectious mononucleosis in only one case with significant EBV antibody rise, seroreversion in three cases in early childhood, higher newborn than maternal EBV antibody titres in three cases and the establishment of two permanent lymphoblastoid cell lines from newborns following exchange transfusion raise the possibility of abortive primary EBV infection in early life. Congenital or neonatal infections following exchange transfusions, however, could not be substantiated with certainty since the EBV antibodies did not persist at follow-up except possibly in two cases. Parenteral transmission of the EB virus by exchange transfusion at birth is probably prevented by the presence of EBV antibodies in either donor or recipient.


Epstein-Barr virus infections.

Epstein-Barr virus infections.


Relationship of antigenicity of melanoma cells grown in 5-bromodeoxyuridine to reduced tumorigenicity.


Polyadenylate-containing RNA of polyribosomes isolated from rat liver and Morris hepatoma 7800.


Comparison of surface material, cytoplasmic filaments, and intercellular junctions from untransformed and two mouse sarcoma virus-transformed cell lines.


Ultrastructural comparison between the distribution of concanavalin A and wheat germ agglutinin cell surface receptors of normal and transformed hamster and rat cell lines.


Enhancement of adenovirus transformation by treatment of hamster embryo cells with diverse chemical carcinogens.


Dietary enhancement of nitrosamine carcinogenesis.


Rapid two-dimensional immunoelectrophoresis of human serum proteins.


A light and electron microscopic study in serial sections of dystrophic extraocular muscle fibers.


Human adrenal tumors and hyperplasias in vitro: a bridge between morphology and function.


Oxytyrosinase.

Oxytyrosinase.


Relation between the ribosomal sites involved in initiation and elongation of polypeptide chains. Evidence for two guanosine triphosphatase sites.


Isolation and properties of a ribosome-bound factor required for ppGpp and ppGpp synthesis in Escherichia coli.


Regulatory properties of perchlorate-extracted succinate dehydrogenase.


Amino acid sequence of cytochrome c3 from Desulfovibrio vulgaris.


Early photoinduced enzymes of photoinduced carotenogenesis in a Mycobacterium species.


Hormone receptors. 3. Properties of glucagon-binding proteins isolated from liver plasma membranes.


Self-association and allosteric properties of glutamine-dependent carbamyl phosphate synthetase. Reversible dissociation to monomeric species.


Regulated in vitro synthesis of Escherichia coli tryptophan operon messenger ribonucleic acid and enzymes.


Effects of ouabain on catecholamine-stimulated sodium transport in turkey erythrocytes.


Reversible dissociation of the monomer of glutamine-dependent carbamyl phosphate synthetase into catalytically active heavy and light subunits.


Adenosine 3':5'-monophosphate-dependent protein kinase from yeast.


A proton relaxation rate study of the copper analog of Escherichia coli alkaline phosphatase.


The structure of the abortive diphosphopyridine nucleotide-pyruvate-lactate dehydrogenase ternary complex as determined by proton magnetic resonance analysis.


Characterization of the serum high density lipoprotein and apolipoproteins of pink salmon.


Blocks in ganglioside synthesis in transformed hamster cells and their revertants.


Angiotensin II receptors in bovine adrenal cortex. Modification of angiotensin II binding by guanyl nucleotides.


The nature of myelinated nerve fiber degeneration in dominantly inherited hypertrophic neuropathy.


The role of cyclic AMP in mesenteric vasodilation.


Involvement of alpha-receptors in clonidine-induced inhibition of transmitter release from central monoamine neurones.


Dopaminergic denervation supersensitivity and dopamine stimulated adenyl cyclase activity.


The influence of 1-(5-oxohexyl-)3.7-dimethylxanthine (BL 191) on the endocrine and exocrine pancreatic function in man during and following secretin and cholecystokinin-pancreozymin stimulation.


Presence of collagenase from Clostridium histolyticum in gingival sulcal debris of a primitive population.


Evaluation of collagenase activity in the crevicular fluid of children.


Paradoxical increase in rate of catabolism of low-density lipoproteins after hepatectomy. It has been suggested that the liver may be a major site for irreversible degradation of low-density lipoprotein (LDL). The disappearance of autologous (125)I-labeled LDL from plasma was compared in intact and in hepatectomized swine. Contrary to expectations, the rate of irreversible removal of LDL from plasma was increased rather then decreased by hepatectomy. These studies suggest that the liver is not a major site for LDL removal. We propose further that the liver (or some function requiring an intact liver) may affect the metabolism of LDL in a manner that prolongs its lifetime in the plasma compartment.


Cyclic adenosine monophosphate: selective increase in caudate nucleus after administration of L-dopa. Treatment with the dopamine precursor L-dopa produced a significant accumulation of adenosine 3',5'-monophosphate (cyclic AMP) in the caudate nucleus of the rat. In contrast, there was no change in the amount of cyclic AMP in the cerebellum. Accumulation of cyclic AMP in the caudate nucleus after administration of L-dopa was prevented by prior treatment with the decarboxylase inhibitor RO 4-4602. These observations and those in other laboratories support the assumption that dopamine formed from L-dopa selectively activates striatal adenylate cyclase. The in vivo activation of adenylate cyclase after treatment with L-dopa may be a useful model for studying neurological and psychiatric disorders that are thought to involve the dopaminergic system of the brain.


Acute gastroenteritis associated with echovirus type 1.


The effect of 1-nitro-9-aminoacridine on respiration and glycolysis in Ehrlich ascites tumour cells.


Alkylation of cytidine-5'-phosphate: mechanisms of alkylation, influence of O'-alkylation on susceptibility of pyrimidine nucleotides to some nucleolytic enzymes, and synthesis of 2'-O-alkyl polynucleotides.


Preparative photochemical synthesis of isoguanosine ribo- and deoxyribonucleosides and nucleotides, and isoguanosine-3',5'-cyclic phosphate, a new cAMP analogue.


The effect of ethanol on hemostatic properties of human blood platelets.


Manganese induced histochemical and histological alterations in gastrointestinal mucosa of guinea pigs.


A correlation study between adrenal function and the duration of sustained ACTH treatments in the rat.


Clinical manifestations of hepatoma. A review of 6 years' experience at a cancer hospital.


Action of polymyxin B on bacterial membranes. 1. Binding to the O-antigenic lipopolysaccharide of Salmonella typhimurium.


The Child Mental Health Specialist: a new profession.


Quantitation of bovine papilloma virus and serum antibody by immunodiffusion.


Family structure, aftercare, and post-hospital adjustment.


Mixed leukocyte culture test in dogs.

Mixed leukocyte culture test in dogs.


Biological characteristics and viral susceptibility of a rat embryonic skin cell line (RESI).


The use of passive hemagglutination for the detection of bluetongue viral antibodies.


The politics of empiricism. Research recommendations of the Joint Commission on Mental Health of Children.


Morphologic effects of minocycline on the cochlea of the guinea pig.


Ultrastructural study of clostridial myositis.


Pituitary gland in patients with Hurler syndrome. Light and electron microscopic study.


Improved purification and further characterization of Clostridium perfringens type A enterotoxin.


Immunofluorescence of peritoneal phagocytes after infection in vivo with egg-attenuated Chlamydia psittaci 6BC.


Mode of action of N6-O2'-dibutyryl cyclic 3',5' AMP on fat cell metabolism.


Antilipolytic action of tolbutamide in isolated fat cells of the rat.


Induction of liver and lung tumours in rats by the simultaneous administration of sodium nitrite and morpholine.


The structure of ferrocytochrome c at 2.45 A resolution.


Human high density lipoprotein, apolipoprotein glutamine II. The immunochemical and lipid-binding properties of apolipoprotein glutamine II derivatives.


Adenosine cyclic 3':5'-monophosphate-dependent protein kinase from rabbit erythrocytes. Purification and characterization of multiple forms.


Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. I. Effect of nuclear mutations on mitochondrial protein synthesis.


Hormonal control of glycogenolysis and gluconeogenesis in isolated rat liver cells.


Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. II. Effect of nuclear and extrachromosomal mutations on the formation of cytochrome c oxidase.


Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. 3. A nuclear mutant lacking mitochondrial adenosine triphosphatase.


Stoichiometry of adenosine triphosphate-driven proton translocation in bovine heart submitochondrial particles.


Protein hydration changes in the formation of the nicotinamide adenine dinucleotide complexes of glyceraldehyde 3-phosphate dehydrogenase of yeast. I. Buoyant densities, preferential hydrations, and fluorescence-quenching titrations.


Protein hydration changes in the formation of the nicotinamide adenine dinucleotide complexes of glyceraldehyde 3-phosphate dehydrogenase of yeast. II. The spin lattice relaxation of solvent water protons.


Intracellular localization of enzymes of arginine metabolism in Neurospora.


Sheep liver 6-phosphogluconate dehydrogenase. Inhibition by nucleoside phosphates and by other metabolic intermediates.


Regulation of steroidogenesis by adrenocorticotropic hormone in isolated adrenal cells of rats.


Chemical and enzymatic evidence for the participation of a 2-carboxy-3-ketoribitol-1,5-diphosphate intermediate in the carboxylation of ribulose 1,5-diphosphate.


Site-directed spin labeling of the mitochondrial membrane. Synthesis and utilization of the adenosine triphosphatase inhibitor (N-(2, 2, 6, 6-tetramethyl-piperidyl-1-oxyl)-N'-(cyclohexyl)-carbodiimide).


Evidence for a dissociable glucagon binding site in a solubilized preparation of myocardial adenylate cyclase.


The involvement of ribonuclease I, ribonuclease II, and polynucleotide phosphorylase in the degradation of stable ribonucleic acid during carbon starvation in Escherichia coli.


A general method of tritium labeling utilizing microwave discharge activation of tritium gas. Methodology and application to biological compounds.


Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Purification and characterization.


Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Assay and steady state kinetics.


Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Effect of nicotinamide adenine dinucleotide and related compounds on the enzyme-catalyzed arsenolysis of 1,3-diphosphoglyceric acid.


The nucleotide sequence adjoining the CCA end of an Escherichia coli tyrosine transfer ribonucleic acid gene.


The mechanism of action of adrenocroticotropic hormone. The role of mitochondrial cholesterol accumulation in the regulation of steroidogenesis.


Angiotensinase activity of dipeptidyl aminopeptidase I (cathepsin C) of rat liver.


Effect of cyclic adenosine 3':5'-monophosphate on ribosomal protein phosphorylation in reticulocytes.


Purification and properties of phosphoribosyl pyrophosphate synthetase from rat liver.


Estimation of the fructose 1,6-diphosphatase-phosphofructokinase substrate cycle and its relationship to gluconeogenesis in rat liver in vivo.


Purification of adenohypophyseal plasma membranes and properties of associated adenylate cyclase.


Stability and regulation of phosphoribosyl pyrophosphate synthetase from rat liver.


Phosphorylation of adenohypophyseal plasma membranes and properties of associated protein kinase.


Nonenzymatic formation of guanosine 3':5'-monophosphate from guanosine triphosphate.


Fractionation of 34 S ribonucleic acid subunits from oncornaviruses on polyuridylate-sepharose columns.


Properties of follicle-stimulating hormone-receptor interactions. Specific binding of human follicle-stimulating hormone to rat testes.


Evidence for interdependent action of glucagon and nucleotides on the hepatic adenylate cyclase system.


A gonadotropin-responsive adrenocortical adenoma.


TSH stimulation of 32P incorporation into phospholipids of thyroids from patients with Graves' disease.


Control of refractory fasting hypoglycemia in a patient with suspected insulinoma with diphenylhydantoin.


Estrogen receptor concentration in normal and pathological human uterine tissues.


Endocrine function in an anencephalic infant.


Cyclic AMP accumulation and steroidogenesis in the human corpus luteum: effect of gonadotropins and prostaglandins.


Rhesus leukocyte-associated herpesvirus. I. Isolation and characterization of a new herpesvirus recovered from rhesus monkey leukocytes.


Rhesus leukocyte-associated herpesvirus. II. Natural and experimental infection.


Evaluation of cholinesterase-restoring agents in the treatment of tetanus in man.


Viruses causing common respiratory infections in man. 3. Respiratory syncytial viruses and coronaviruses.


Effect of adenine arabinoside on severe Herpesvirus hominis infections in man.


Treatment of herpesvirus encephalitis with iododeoxyuridine.


Ecthyma gangrenosum produced by Aeromonas hydrophilia.


Letter: Bilateral eventration of the diaphragm in a neonate with congenital cytomegalic inclusion disease.


Iatrogenic Cushing's disease in a boy after misdiagnosis of salt-losing virilizing adrenal hyperplasia: impaired metyrapone response with failure of catch-up growth.


Iodide goiter following lymphography.

Iodide goiter following lymphography.


Hepatitis B antigen: removal from protein solutions in a recognizable form by an insoluble lipophilic matrix of silicic acid.


Enzymatic hydrolysis of nucleoside phosphoramidates.


Chemical structure and absolute configuration of a juvenile hormone from grasshopper corpora allata in vitro.


Cholesterol in cerebrospinal fluid of brain tumor patients.


Fatty acid metabolism in denervated muscle of frog in vitro.


Congenital cytomegalovirus infection: consecutive occurrence due to viruses with similar antigenic compositions.


Pharmacological interaction between cannabidiol and delta 9-tetrahydrocannabinol.


Cyclic AMP and its relation to clinical chemistry.


Urinary cyclic AMP excretion in toxaemia of pregnancy.


Pretreatment classification and staging of bronchogenic carcinoma.


Comparative electron microscopic studies on Pseudomonas aeruginosa endotoxin.


Sleep of distance runners.

Sleep of distance runners.


Ultrastructural study of neuronal cytoplasmic inclusions produced by hypocholesterolemic drug, AY 9944.


Creutzfeldt-Jakob disease: a histochemical study with microdensitometric assay of some enzymes.


Chemistry of iron in biological systems.


90Y ferric hydroxide colloid.

90Y ferric hydroxide colloid.


Ribonuclease present in myxoviruses.

Ribonuclease present in myxoviruses.


Retention of 90Y in patients with rheumatoid arthritis.


On the receptor of influenza viruses. 1. Artificial receptor for influenza virus.


Organizational history of HSMHA.

Organizational history of HSMHA.


Diaminopurine-resistant mutants of cultured, diploid human fibroblasts. Clones of cells resistant to 2,6-diaminopurine were detected in skin fibroblast cultures derived from 13 of 21 normal humans of both sexes from 17 unrelated families. Almost all of the cultures that yielded mutants were chosen for further study from among a total of 83 surveyed because they displayed a slight resistance to low concentrations of diaminopurine. The incidences of mutant colonies ranged between about 10(-5) and 10(-4) per cell surviving prior mutagenic treatment with MNNG. The incidences of spontaneous mutants were about 10(-7) to 10(-5) in three unrelated cultures. Most independent mutants had distinctly reduced activity of adenine phosphoribosyltransferase but some had apparently normal amounts of activity. Two mutants from unrelated boys had little or no detectable enzyme activity and were unable to effectively use exogenous adenine for growth when purine biosynthesis was blocked with azaserine. Most mutants could utilize exogenous adenine, just as most azaguanine-resistant fibroblast mutants can utilize exogenous hypoxanthine, even when their hypoxanthine-guanine phosphoribosyltransferase activity is reduced. Diverse genetic changes conferred diaminopurine resistance but their specific natures are still undefined. Gross numerical or structural chromosome abnormalities were not observed in the mutants examined so far. Since at least one gene responsible for adenine phosphoribosyltransferase activity is on autosome No. 16 our results suggest that at least some of the cultures yielding mutants were heterozygous and that alleles conferring diaminopurine resistance may be frequent enough to comprise a polymorphism.


The NIMH approach to evaluating the community mental health centers program.


Electrotonic conduction in the optic nerves of planorbid snails.


Capacitation of epididymal spermatozoa in a medium with high K-Na ratio and cyclic AMP for the fertilization of rat eggs in vitro.


Oestradiol-17beta: its influence on cyclic 3'5'-adenosine monophosphate phosphodiesterase and 5'-nucleotidase in the cervicovaginal epithelium of neonatal mice.


Acute localized amebic appendicitis: report of a case.


Early components of the averaged electroencephalic response to constant level clicks during rapid eye movement sleep.


Multilocular cysts of kidney.

Multilocular cysts of kidney.


Renal cystography with triple-contrast material.


Plasma renin activity. Comparison of bioassay and radioimmunoassay techniques.


Adrenalectomy and hypophysectomy for prostatic cancer.


Alkaline and acid phosphatase activity in buffalo semen.


Adeno, parainfluenza, RS, and corona viruses and Mycoplasma pneumoniae as etiologic agents of acute respiratory tract infections.


Serological surveillance of respiratory viruses in healthy population of the Koszalin Province.


Seroepidemiological studies on the occurrence of several respiratory viruses in the population of the Lublin province.


Typhoid-paratyphoid (TAB) vaccine as interferon inducer in mice.


Role of incomplete viruses and interferon in persistent infection of mouse cells with Sindbis virus.


The in vitro effect of cyclophosphamide on Col. MM virus replication and interferon production.


Antiviral drugs in human therapy.

Antiviral drugs in human therapy.


Studies on antiviral activity of alpha-trihalogenomethylamines.


Latent virus infections in primary monkey kidney cultures.


Kinin-converting aminopeptidase from human serum.


A comparative study of purine metabolism by human and pig erythrocytes in vitro.


Effects of adrenochrome and sodium perborate on isolated fat cell metabolism.


Effects of chlorpromazine, thioridazine and haloperidol on adrenergic transmitter mechanisms in man.


Comparative effect of vincristine and alkylating agent No. 864 on rat leukemia in relation to the distribution of drugs and leukemic cells.


Distribution of 51Cr-labeled lymphoid cells in the lymph node draining the inoculated tumor in the rat.


Synergistic effects of bis(3-methylsulfonyloxypropyl)-amine toluenesulfonate (864-T) with 6-mercaptopurine and other antitumor agents.


Type-C virus in cultured cells derived from normal and tumor cells of a rat.


Hepatoblastoma.

Hepatoblastoma.


Surgical anatomy of the intraglandular portion of the facial nerve.


Cytoplasmic and nuclear pyrimidine ribonucleotide pools in HeLa cells.


Experiences with vulvar cancer.

Experiences with vulvar cancer.


Inactivation of poliovirus in water by ozonation.


Trichinosis--the Maryland outbreak of 1970.


Isolation and purification of surface antigens from disrupted paramyxoviruses.


The influence of polymyxin B on virus-induced cytopathogenic effect.


Mitochondrial DNA synthesis in Raji cells infected by herpes simplex virus or Epstein-Barr virus.


Recovery of the antibody-forming potential in cyclophosphamide-treated mice, with special reference to the influence of Bordetella pertussis.


Neurotransmitting substances and endotoxin respone in relation to hepatic enzyme induction.


Histochemical investigations into the lipid nature of the oil-droplet in the retinal twin-cones of Lebistes reticulatus (Peters). (With 5 figures).


Studies on RNA metabolism during lymphocyte activation.


Embryogenesis of Calliphora erythrocephala. 3. Ultrastructure of the midgut epithelial cells during late embryonic development.


Histochemical and fine structure observations of the intestinal epithelium of Trichuris suis (Nematoda: Trichuroidea).


The fine structure of the spermatozoon of Littornia (Gastropoda: Prosobranchia), with special reference to sperm motility.


CSF contacting axons and synapses in the lumen of the pineal organ.


Ultrastructural studies of the commensal suctorian, Choanophrya infundibulifera Hartog. I. Tentacle structure, movement and feeding.


Ultrastructure of STH cells of the pars distalis of castrated male mice after hepatectomy.


Ultrastructural studies of the commensal suctorian, Choanophrya infundibulifera Hartog. II. Tentacle morphogenesis.


Structural connections between dense core vesicles in the central nervous system of Anodonta cygnea L. (Mollusca, Eulamellibranchia).


Replication of swinepox virus in the skin of naturally infected pigs. Electron microscopic study.


A guide to bone scanning.

A guide to bone scanning.


Home care after peripheral vascular surgery.


Spectrophotometric determination of free silica.


The effect of pattern movement on the impulse activity of the cervical connective of Drosophila melanogaster.


Intracerebral hemorrhage as initial symptom of a brain tumor.


Suppression of plasma ACTH levels with corticosteroids in Addison's disease.


Production of a corticotrophin-like intermediate lobe peptide and of corticotrophin by a bronchial carcinoid tumour.


Plasma unconjugated and conjugated 17-hydroxycorticosteroids in different clinical disorders.


The histological classification of malignant lung tumors.


ACTH and MSH assays and their clinical application.


Establishing an alcoholism treatment program in a community hospital.


Human cerebral blood flow during sleep and waking.


Studies on the circadian rhythm of phosphoenolpyruvate carboxykinase activity in rats. II. Effect of the autonomic nervous system on the rhythm in liver.


Glyceraldehyde 3-phosphate dehydrogenase of Bacillus stearothermophilus. Kinetics and physicochemical studies.


Interaction of yeast alcohol dehydrogenase with various substrates. I. Binding of coenzymes.


Disseminated herpesvirus infection in a rhesus monkey (Macaca mulatta).


The differential capacity of a nerve channel.


Response of specific skin hypersensitivity and haemagglutination inhibiting antibody after smallpox vaccination in human newborns and adults.


Effects of chronic alcohol abuse on the fatty acid composition of major lipids in the human brain. Hepatocerebral degeneration II.


Cerebrospinal fluid cholesterol. Quantitative analysis of unesterified and esterified cholesterol and normal values in the cerebrospinal fluid.


Cerebral lipids in experimental cyanide encephalopathy.


Correlation between hypothalamic serotonin content and adrenal function during acute stress. Effect of adrenal corticosteroids on hypothalamic serotonin content.


Staphylococcus epidermidis isolated from bovine milk. Biochemical properties, phage sensitivity and pathogenicity for the udder.


The kinetic and redox potentiometric resolution of the carotenoid shifts in Rhodopseudomonas spheroides chromatophores: their relationship to electric field alterations in electron transport and energy coupling.


Biochemical and biophysical studies on cytochrome c oxidase. XI. Reaction with azide.


Mitochondrial metabolism in porphyric rat liver.


Biochemical and biophysical studies on cytochrome c oxidase. XII. Kinetics of azide binding.


A new cytochrome b-like pigment with a peak at 567 nm and a low redox potential in denitrifying bacteria.


A simple method for the purification of commercial neuraminidase preparations free from proteases.


Studies on a pyrophosphatase and glucose-6-phosphatase from Aspergillus oryzae.


Letter: Singlet oxygen decontaminating activity of erythrocuprein (superoxide dismutase).


Prednisolone for idiopathic (Bell's) facial palsy.


Conformational changes of aspartate aminotransferases in the region of Cys-45 residue observed by means of spin label.


Molecular properties of lactic dehydrogenase under the conditions of the enzymatic test. Sedimentation analysis and gel filtration in the microgram and nanogram range.


Synthesis of glucose-6-phosphate by a I,3-diphosphoglycerate-glucose phosphotransferase activity of liver microsomes.


Pathways for the oxidation of malate and reduced pyridine nucleotide by wheat mitochondria.


Effect of temperature on the behavior of rat-liver mitochondria during centrifugation in a sucrose gradient.


Effects of hypnotic induction and suggestions on nocturnal dreaming and thinking.


Equine infectious anemia: a retrospective study of an epizootic.


Combined (B- and T-lymphocyte) immunodeficiency: a fatal genetic disease in Arabian foals.


Carotid baroreceptor modulation of diaphragm and abdominal muscle activity in the cat.


Intranuclear rods and sheets in rat cochlear nucleus.


The fine structure of freeze-fractured presynaptic membranes.


Aggregations of filaments in Schwann cells of spinal roots of the normal rat.


Editorial: Analysis of congestive heart failure in terms of ventricular function curves.


Alcohol and the blood.

Alcohol and the blood.


The effect of cyclic AMP and aminophylline on Limulus lateral eye retinular cells.


Effect of renal pressor systems on vascular capacity during hemorrhage.


Respiratory characteristics of the cabbage butterfly during different stages of larval development.


Characterization of smooth muscle receptors for angiotensin: studies with an antagonist.


Glucose metabolism in jejunal mucosa of fed, fasted, and streptozotocin-diabetic rats.


Metabolic effects of ACTH in the sheep.

Metabolic effects of ACTH in the sheep.


Cytochrome c and evolution of the energy acquiring system.


Protocollagen proline hydroxylase activity in work-induced hypertrophy of rat muscle.


Hormonal and dietary adaptation of rat pancreatic hydrolases before and after weaning.


Using cooperative extension programs for health education.


Evidence for adrenocortical uptake of ACTH in vivo.


Useful antiviral drugs.

Useful antiviral drugs.


Use of stable, sensitized cells in indirect micro hemagglutination test for amebiasis. Human "O" cells were fixed with pyruvic aldehyde, treated with tannic acid, and fixed with glutaraldehyde. The cells were sensitized with amoeba antigen and stored in a refrigerator. The sensitized cells were used periodically for the indirect hemagglutination test with a battery of sera from patients with intestinal amebiasis and confirmed and unconfirmed amebic liver abscess, and also from negative controls. The same battery was tested with cells sensitized with different batches of antigen and also with fresh sheep cells. None of the cells showed any reaction with negative control sera. The fixed cells remained sensitive and stable throughout the study. Reproducibility of the titers with the fixed cells within each day and from day to day was satisfactory. The titers with fixed human "O" cells were slightly lower than were the titers with fresh sheep cells. The advantages of using stable, sensitized cells are pointed out.


Antibody to viruses affecting cattle in commercial tissue culture grade fetal calf serum. Commercial fetal calf serum (FCS) for tissue culture use was tested for neutralizing activity against several viruses which affect cattle. Certain lots of FCS contained no neutralizing activity, whereas other lots contained neutralizing activity to several viruses. It was concluded that the neutralizing activity found in certain lots of sera was due to specific antibody and that its presence could be most easily explained by the contamination of the FCS with serum from postcolostral bovine serum. A nonantibody inhibitor to vesicular stomatitis virus was also found at low levels in most lots of serum. Because those sera which had antibody had antibody to several viruses, it was suggested that the use of the micro-serum neutralization test with a few bovine viruses which are widespread in the bovine population should be satisfactory to detect FCS which was contaminated with postcolostral bovine serum.


Methods for visualization of enzymes in polyacrylamide gels. White bands resulting from precipitation of dodecan-1-ol liberated by hydrolysis of sodium dodecyl sulfate and decan-5-ol released by hydrolysis of decan-5-yl sulfate produced zymograms of the primary and secondary alkylsulfatases from Pseudomonas C(12)B. Gas-liquid chromatographic analyses of ether extracts of the precipitate-containing segments of the zymograms confirmed the identity of the alcohols which were not discerned in extracts of segments of the gels other than those containing precipitates. beta-Galactosidase from Escherichia coli was marked on zymograms by the liberation of o-nitrophenol from o-nitrophenyl-beta-D-galactoside, and arylsulfatase from Pseudomonas C(12)B was marked in gels by liberation of p-nitrophenol from p-nitrophenyl sulfate. Membrane-associated dissimilatory nitrate reductases from a nitrate respirer (Enterobacter aerogenes) and a denitrifier (Pseudomonas perfectomarinus) did not penetrate either 6.8 or 3% polyacrylamide gel but were demonstrable at the top of the gels. In the membrane-bound state, formate served as electron donor for nitrate reductase from E. aerogenes, and reduced nicotinamide adenine dinucleotide (NADH) served as donor for nitrate reductase from P. perfectomarinus. Both enzymes reduced nitrate at the expense of reduced benzyl viologen as well. Assimilatory nitrate reductase from E. aerogenes moved easily into the 6.8% gels (R(f) = 0.43 under the conditions of these experiments). The reduced dye served as electron donor for the assimilatory reductase, but formate and NADH did not. Incubation of the membrane-associated nitrate reductases with 2% Triton X-100 solubilized the enzymes and removed the capacity of formate and NADH to serve as electron donors. Both retained the ability to reduce nitrate at the expense of reduced benzyl viologen. The solubilized dissimilatory reductase from E. aerogenes moved further in the gels (R(f) = 0.49) than the soluble assimilatory reductase; the solubilized dissimilatory reductase from the denitrifier, P. perfectomarinus, moved further in the gels (R(f) = 0.64) than either of the enzymes from E. aerogenes.


Detection of bacteremia with liquid media containing sodium polyanetholsulfonate. Two liquid blood culture media, Tryptic soy broth (TSB) and Thiol broth, containing sodium polyanetholsulfonate were compared in 8,654 cultures. Pseudomonas and Corynebacterium (including Propionibacterium) were isolated significantly more frequently (P < 0.001) from TSB than from Thiol. Escherichia coli, Haemophilus, and Bacteroidaceae were isolated more frequently in TSB; however, the differences were not statistically significant. In no instance was Thiol superior to TSB in detecting bacteremia. In an additional 2,977 cultures, aerobic and anaerobic Vacutainer culture tubes with supplemented peptone broth were inoculated in parallel with TSB and Thiol. Significantly greater rates of detection (P < 0.01) in TSB or Thiol were noted with Pseudomonas, E. coli, Enterobacter, viridans, and group A streptococci, Bacteroidaceae, and staphylococci.


Microtiter hemagglutination-inhibition test for the detection of encephalomyocarditis virus antibodies. Several variables were found to affect the agglutination of sheep erythrocytes by encephalomyocarditis virus. A satisfactory and reliable microtiter hemagglutination-inhibition test is described.


Evaluation and modifications of media for enumeration of Clostridium perfringens. The suitability of the Shahidi-Ferguson perfringens, TSC (tryptose-sulfite-cycloserine), and oleandomycin-polymyxin-sulfadiazine perfringens agars for presumptive enumeration of Clostridium perfringens was tested. Of these, the TSC agar was the most satisfactory. The TSC agar method was improved by eliminating the egg yolk and using pour plates. The modified method allowed quantitative recoveries of each of 71 C. perfringens strains tested and is recommended. For confirmation of C. perfringens, the nitrite test in nitrate motility agar was unreliable, particularly after storage of the medium for a few days. In contrast, positive nitrite reactions were obtained consistently when nitrate motility agar was supplemented with glycerol and galactose.


Inactivation of encephalomyocarditis virus in aerosols: fate of virus protein and ribonucleic acid. After aerosolization at relative humidities of 50% or lower, encephalomyocarditis virus is rapidly inactivated. In this process the protein coat of the virion is damaged. This appears as a loss of hemagglutination activity and loss of affinity for hemagglutination inhibiting antibodies. The ribonucleic acid of the virus retains its infectivity but it becomes susceptible to ribonuclease. It sediments in sucrose gradients when centrifuged at high speed with the same velocity as free infectious ribonucleic acid extracted with phenol from intact encephalomyocarditis virus.


A follow-up of monozygotic twins discordant for schizophrenia.


Basal cell adenoma of the parotid gland.


Intramitochondrial filamentous inclusions in chronic glomerulonephritis.


Mallory bodies and giant mitochondria--two different structures in liver biopies from alcoholics.


The mechanism of action of the respiratory inhibitor, antimycin.


The different cytochrome b components in the respiratory chain of animal mitochondria and their role in electron transport and energy conservation.


Odontodysplasia.

Odontodysplasia.


Oral medicine for the dental practitioner. 4. Recurrent ulcers.


The separation of human B lymphocytes on a digestible immunoadsorbent column.


Operative and cytostatic treatment of germinal tumours of the testis.


Phospholipids in the cytochrome oxidase reaction.


Effect of estrogens on the mast cell degranulating action of compound 48-80.


Sulfhydryl groups of cytochrome oxidase.


Selective cytotoxicity of peritoneal leucocytes. A preliminary report.


Some properties of a homocarnosine-carnosine synthetase isolated from rat brain.


Electrophoretic characterization of leucine, glucosamine- and fucose-labelled proteins rapidly transported in frog sciatic nerve.


Chronic hypnotic-drug use. Ineffectiveness, drug-withdrawal insomnia, and dependence.


Editorial: Insomnia.

Editorial: Insomnia.


Massive hyperlipemia during estrogen therapy.


Avian leukosis-sarcoma virus antibodies in wildfowl, domestic chickens and man in Kenya.


Measurement of human red cell angiotensinase activity by angiotensin II radioimmunoassay.


Activation of type C RNA tumor virus expressions in tumors induced by cell cultures transformed by polyoma virus.


Neutralizing antibody against infectious canine hepatitis virus (ICHV) in veterinary workers' sera.


Effect of sex and sex hormones on plasma prothrombin and vitamin K deficiency.


Spontaneous in vitro transformation of leukocytes from a neonate.


Cytochalasin D enhances infectivity of poliovirus.


The growth of varicella-zoster virus in guinea pig embryo cells.


Remarks on mammographic calcifications.

Remarks on mammographic calcifications.


Effects of corticosterone on phosphorylation of rat liver nuclear proteins in vitro.


Kinetics of the phenotypic expression of mutations for streptomycin resistance induced by methylmethane sulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, and ultraviolet light in Escherichia coli K-12.


Cytogenetic effect of alkylating mutagens as a function of the physiological state of seeds.


Renal trauma and preexisting lesions of kidney.


Products of mitochondrial protein synthesis.


In vitro effects of prolactin and cortisol on water permeability of the urinary bladder of the teleost Gillichthys mirabilis.


Ultrastructural effects of dark-adaptation on eyes of a snail, Helix aspersa.


What is your diagnosis? Mixed tumor.

What is your diagnosis? Mixed tumor.


Cleavage initiating activities of sperm fractions injected into the egg of the medaka, Oryzias latipes.


Statewide policies for planning nursing education in Minnesota.


An investigation of hydrolytic techniques for the amino acid analysis of foodstuffs.


Dopamine-induced inhibition of synaptic transmission in lumbar paravertebral ganglia of the dog.


Effect of vasopressin administration on renal haemodynamic changes in rats treated with ACTH.


Primary herpetic gingivostomatitis in the adult.


The action of unconjugated bilirubin on some enzyme activities of in vitro cultured cells.


Intraneuronal lipid droplets in irradiated nervous tissue.


Ultrastructure of the protein containing vacuoles and hyaline droplets in rat adrenocortical cells following polybrene.


Acute renal failure in obstetrics.

Acute renal failure in obstetrics.


Action of metabolic hormones on the fine structure of liver cells. II. Effects of hypophysectomy and chronic administration of somatotropin.


Malignant epithelial tumors of the parotid gland. Classification, staging and treatment.


Immunologic response in gynecologic malignancy as demonstrated by the delayed hypersensitivity reaction: clinical correlations.


Karyotypic variation in benign pleomorphic adenoma of the parotid and in normal salivary glands.


Studies on the combined action of antibiotics on Pseudomonas aeruginosa strains.


Studies on neutral esters of cyclic AMP.


Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma.


Nucleotide sequence analysis of DNA. XII. The chemical synthesis and sequence analysis of a dodecadeoxynucleotide which binds to the endolysin gene of bacteriophage lambda.


Binding of trifluorolactate to pig heart H4-lactate dehydrogenase.


Some properties of coupled hepatoma mitochondria exhibiting uncoupler-insensitive ATPase activity.


Biochemical studies of otosclerosis. Phosphohydrolase activity in stapedial footplates.


Analysis of amino acid phenylthiohydantoins by high speed liquid chromatography.


Alteration of NAD-linked malic enzyme-catalyzed reactions by sulfhydryl group modification.


Model reactions for the study of the interaction of sulfur dioxide with mammalian organisms.


Carbamylphosphate synthetase of lactating bovine mammary tissue.


A specific spin labeling of the anticodon of E. coli tRNA-Glu.


Cyclic AMP phosphodiesterase activation by the histamine releasing agent, compound 48-80.


Polychlorinated biphenyls: hydroxylated urinary metabolites of 2,5,2',5'-tetrachlorobiphenyl identified in rabbits.


Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteins.


Prediction of protein conformation.

Prediction of protein conformation.


Estrogen receptor binding to isolated nuclei. A nonsaturable process.


Changes in sulfhydryl groups of honeybee glyceraldehyde phosphate dehydrogenase associated with generation of the intermediate plateau in its saturation kinetics.


Glucocorticoid receptors in mouse pituitary tumor cells. II. Nuclear binding.


Monomeric and dimeric forms of component II of the anthranilate synthetase--anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase complex of Salmonella typhimurium. Implications concerning the mode of assembly of the complex.


The dynamic structure of lipid membranes. A 13C nuclear magnetic resonance study using spin labels.


Substrate-attached glycoproteins from normal and virus-transformed cells.


Structure and activity of methylated horse liver alcohol dehydrogenase.


Physical identity of the SV40 deoxyribonucleic acid sequence recognized by the Eco RI restriction endonuclease and modification methylase.


A filter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrant.


Human placental tryptophanyl transfer ribonucleic acid synthetase. Purification and subunit structure.


Derivatives of N-(N-(9-beta-D-ribofuranosylpurin-6-yl)carbamoyl)threonine in phosphodiesterase hydrolysates of wheat embryo transfer ribonucleic acid.


Malignant fibrous histiocytoma with widespread metastases. Autopsy study.


Human tryptophanyl transfer ribonucleic acid synthetase. Comparison of the kinetic mechanism to that of the Escherichia coli tryptophanyl transfer ribonucleic acid synthetase.


Mucinous adenocarcinoma of the renal pelvis. Report of two cases.


Elastosis in breast cancer.

Elastosis in breast cancer.


Familial arrhenoblastoma and thyroid adenoma.


Malignant chemodectoma presenting as a miliary pulmonary infiltrate.


Studies on the quaternary structure of the first enzyme for histidine biosynthesis.


On the mechanism of the inhibitory action of 2-amino-4,6-dichloropyrimidine on poliovirus growth.


Irreversible impairment produced by guanidine on the functions of poliovirus proteins.


Morphological and enzymehistochemical changes in the interscapular adipose tissue of adult guinea-pigs during prolonged exposure to cold.


Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids. Assignment of PGM3 to chromosome C6 and regional mapping of the PGD, PGM1 and pep-C genes on chromosome A1.


Chromosome assignments of genes in man using mouse-human somatic cell hybrids: mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6.


Syrian hamster-human somatic cell hybrids: isolation and characterization.


Long-term storage, migration and elimination of dust in the lungs of animals, with special respect to the influence of polyvinyl-pyridine-N-oxide.


Influence of BCG on lymphatic lung clearance in rats.


The relationship between duration of dust inhalation of a coal-quartz mixture and dust retention, lung function and pathology on rats.


Lung function studies in experimental silicosis with a radiospirometric technique and some observations on a technique for inducing unilateral silicosis. A preliminary report.


Observations on the mechanism of silicotic fibrogenesis.


Investigations on the aetiology of coal workers' pneumoconiosis with the use of PVN-oxide.


On the cell toxicity of mineral dusts.

On the cell toxicity of mineral dusts.


The cytotoxic action and the semiconductor properties of mine dusts.


The role of some physical and chemical properties of mine dust in the development of pneumoconiosis.


Post-hospital discharge: patient care and utilization of services and facilities.


"The central areas" of denervated canine muscle.


Pathology of Clostridium welchii type D enterotoxaemia. I. Biochemical and haematological alterations in lambs.


Pathology of Clostridium welchii type D enterotoxaemia. II. Structural and ultrastructural alterations in the tissues of lambs and mice.


Spontaneous malignant neoplasms in dasyurid marsupials.


Pathology of Clostridium welchii type D enterotoxaemia. 3. Basis of the hyperglycaemic response.


Pulmonary adenomatosis in ageing mice.

Pulmonary adenomatosis in ageing mice.


Terminal oxidases and carbon monoxide-reacting haemoproteins in the trypanosomatid, Crithidia fasciculata.


Chemistry of the filaments and tubules of brain.


Electronystagmographic comparison of acute idiopathic and herpes zoster facial paralysis.


Letter: Gas gangrene.

Letter: Gas gangrene.


Letter: Potassium, aldosterone and renin inter-relations.


Fatal infectious mononucleosis in a family.


Depolarization generated by acetylcholine through activation of triphosphoinositide phosphomonoesterase.


Physostigmine in choreiform movement disorders.


Evaluation of antithymocyte globulin in acute relapses of multiple sclerosis.


An experimental myopathy secondary to excessive acetylcholine release.


Automatic midline echoencephalography. Examination of 3,333 consecutive cases with the automatic midline computer.


A natriuresis receptor at the carotid bifurcation specifically activated by oxytocin.


A new model for transmitter mobilization in the frog neuromuscular junction.


Angiographic findings in benign liver cell tumors.


A complication of skin testing with iodinated radiographic contrast medium.


Total body radionuclide localization in infants.


A new method for radionuclide imaging of myocardial infarcts.


Pediatric urology.

Pediatric urology.


Pharmacokinetics and dosing of antimicrobial agents in renal impairment. I.


N-acetyl-beta-D-hexosaminidase component A. Different forms in human tissues and fluids. 1. Hexosaminidase A of human serum was resolved into two components, a minor form with properties identical with those of the single hexosaminidase A component of human liver, and a major form with significantly different properties. 2. The major serum hexosaminidase A form was eluted from a DEAE-cellulose column at a lower salt concentration than that required to elute the liver form. 3. A multiple-pass technique was used to elute the major serum enzyme A from a Sephadex G-150 column before that of liver enzyme A. 4. Clostridium perfringens neuraminidase converted the major component of serum hexosaminidase A into a form that was held less tightly by DEAE-cellulose, but the minor component of the A enzyme of serum, and the A enzyme of liver were not affected. 5. The hexosaminidase A from tears was similar to the A enzyme from serum, whereas those from several human tissues and from urine and lymph were similar to the liver form. 6. The A enzyme from serum may be derived from the A enzyme from liver by glycosylation before secretion.


Collagen cross-linking. Isolation of cross-linked peptides from collagen of chicken bone. Cross-linked peptides were isolated from chicken bone collagen that had been digested with CNBr or with bacterial collagenase. Analyses of (3)H radioactivity in disc electrophoretic profiles of the CNBr peptides from bone collagens that had been treated with NaB(3)H indicated that a major site of intermolecular cross-linking in chicken bone collagen is located between the carboxy-terminal region of an alpha1 chain and a small CNBr peptide, probably situated near the amino-terminus of an alpha1 or alpha2 chain in an adjacent collagen molecule. A small amount of this cross-linked CNBr peptide was isolated from a CNBr digest of chicken bone collagen by column chromatography. Amino acid analysis showed that the CNBr peptide, alpha1CB6B, the carboxy-terminal peptide of the alpha1 chain, was the major CNBr peptide in the preparation, and the reduced cross-linking components were identified as hydroxylysinohydroxynorleucine (HylOHNle), with a smaller amount of hydroxylysinonorleucine (HylNle). However, the composition and the low recovery of the cross-linking amino acids suggested that the preparation was a mixture of CNBr peptides alpha1CB6B and alpha1CB6B cross-linked to a small CNBr peptide whose identity could not be determined. A small cross-linked peptide was isolated from chicken bone collagen that had been reduced with NaB(3)H(4) and digested with bacterial collagenase. This peptide was the major cross-linked peptide in the digest and contained a stoicheiometric amount of the reduced cross-linking compounds. A peptide which had the same amino acid composition, but contained the cross-linking compounds in their reducible forms, was isolated from a collagenase digest of chicken bone collagen that had not been treated with NaBH(4). The absence of the reduced cross-links from this peptide indicates that, at least for the cross-linking site from which the peptide derives, natural reduction is not a significant pathway for biosynthesis of stable cross-links. However, most of the reducible cross-linking component in the peptide appeared to stabilize in the bone collagen by rearrangement from aldimine to ketoamine form.


Functional groups in the activity and regulation of Escherichia coli citrate synthase. 1. Citrate synthase has been purified from Escherichia coli and shown to exist at an equilibrium between three forms: monomer (mol.wt. 57000), tetramer (mol.wt. 230000) and, possibly, octamer. Modification of the enzyme by photo-oxidation and by treatment with specific chemical reagents has been carried out to gain information on the amino acid residues involved in enzymic activity and in the inhibition of activity by NADH and alpha-oxoglutarate. 2. Several photo-oxidizable amino acids appear to be involved in activity. The nature of the pH-dependence of their rates of photo-oxidation with Methylene Blue suggests that these are histidines, a conclusion supported by the greater rate of photo-inactivation with Rose Bengal and the destruction of activity by diethyl pyrocarbonate. 3. The participation of histidine at the alpha-oxoglutarate effector site is indicated by photo-oxidation and the participation of cysteine at the NADH effector site suggested by photo-oxidation is confirmed by the desensitization to NADH produced by treatment with 5,5'-dithiobis-(2-nitrobenzoate). Inactivation of the enzyme after modification with this reagent suggests the additional involvement of cysteine in catalytic activity. 4. Amino acid analyses of native and photo-oxidized enzyme are consistent with these conclusions. 5. Modification with 2-hydroxy-5-nitrobenzyl bromide indicates the participation of tryptophan in the activity of the enzyme.


The use of sodium perchlorate in deproteinization during the preparation of nucleic acids. Sodium perchlorate in high concentrations will remove from solution the detergent sodium dodecyl sulphate and protein complexed with it. This and the failure of proteins to be precipitated by ethanol from solutions containing a high concentration of sodium perchlorate can be utilized as efficient, rapid and simple deproteinization procedures during the preparation of nucleic acids.


Phosphorylation of myelin basic protein by an adenosine 3':5'-cyclic monophosphate-dependent protein kinase. Myelin basic protein was shown to be a substrate for protein kinase from rabbit muscle. One of the major sites of phosphorylation was the serine residue in the sequence Gly-Arg-Gly-Leu-Ser-Leu. The arginine residue in this sequence is known to be a substrate for a protein methylase.


Structural investigations of lipid, polypeptide and protein multilayers.


The high molecular weight RNA of Visna virus.


Strychnine interactions with acetylcholine, dopamine and serotonin receptors in Aplysia neurons.


Attention of transmission through glomeruli of olfactory bulb on paired shock stimulation.


Relation of glomerular neuronal activity to glomerular transmission attenuation.


The synthesis of p-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) P2-ficaprenyl pyrophosphate.


Angiography of renal capsular complex in normal and pathological conditions and its diagnostic implications.


Vasodilator reserve, parallel vascular beds, and significant stenosis: a review for the angiographer.


The therapeutic effects of cyclacillin in acute sinusitis: in vitro and in vivo correlations in a placebo-controlled study.


The acute effect of tetracosactrin on carbohydrate, insulin, glucagon, gastrin and lipid metabolism in rheumatoid arthritis.


Inhibition of phosphorylase-a by fructose-1-phosphate, alpha-glycerophosphate and fructose-1,6-diphosphate: explanation for fructose-induced hypoglycaemia in hereditary fructose intolerance and fructose-1,6-diphosphatase deficiency.


The induction of fibrogenesis by silica-treated alveolar macrophages.


Prostaglandins and renal function.

Prostaglandins and renal function.


Prostaglandins as modulators of the autonomic nervous system.


Prostaglandins and the thyroid gland.

Prostaglandins and the thyroid gland.


Ultrastructural observations on human oocytes fertilized in vitro.


A rapid screening procedure for some "street-drugs" by thin-layer chromatography. II. Cocaine, heroin, local anesthetics and mixtures.


Separation of diphosphoinositide and triphosphoinositide on oxalate-impregnated silica gel columns.


Intraoperaoperative echoencephalography.


Control of retinal sensitivity. I. Light and dark adaptation of vertebrate rods and cones. Rods and cones in Necturus respond with graded hyperpolarization to test flashes spanning about 3.5 log units of intensity. Steady background levels hyperpolarize the rods, and the rod responses become progressively smaller as background level is increased. In cones, higher background levels reduce the effectiveness of test flashes, so higher ranges of test intensities are required to elicit the full range of graded responses. When backgrounds are terminated, cones return rapidly, but rods return slowly to the dark potential level. The effects of backgrounds on both rods and cones can be observed at intensities that cause negligible bleaching as determined by retinal densitometry. During dark adaptation, changes are observed in the rods and cones that are similar to those produced by backgrounds. Receptor sensitivities, derived from these results, show that rods saturate, cones obey Weber's law, and sensitization during dark adaptation follows a two-phase time-course.


Control of retinal sensitivity. II. Lateral interactions at the outer plexi form layer. Test stimuli, presented at the center of the bipolar cell receptive field, spanning less than 2 log units of intensity, elicit the full range of graded response. The intensity range of test stimuli that elicits the graded response depends upon the background conditions. A higher range of log test intensities is required to elicit the graded bipolar response in the presence of surround backgrounds. But surround backgrounds can also serve to unsaturate the bipolar response and thereby increase sensitivity under certain conditions. The results suggest that a second stage of sensitivity-control is mediated by the horizontal cell system at the outer plexiform layer, concatenated with the effects of adaptation in the photoreceptors.


Control of retinal sensitivity. 3. Lateral interactions at the inner plexiform layer. Both the "on" and the "on-off" ganglion cells in the mudpuppy retina generate graded responses over a narrow range of log test intensities. Sustained full field or surround backgrounds change the range of center log test intensities that elicits the graded response for both cell types. The on-off, but not the on ganglion cells are further affected by moving or flashing surround backgrounds. These cells are hyperpolarized, threshold is elevated, and the entire graded range of response is elicited by a higher range of log center test intensities. Depolarizing activity is elicited in amacrine cells by moving backgrounds that affect the on-off ganglion cells, but bipolar activity is unaffected. These results suggest that the amacrine cells at the inner plexiform layer mediate a third stage of sensitivity control in the retina, increasing threshold for response to change specifically in the on-off ganglion cells.


A systematist looks at cytochrome c.

A systematist looks at cytochrome c.


Sequence data: magnitude and implications of some ambiguities.


Serotonergic and antiserotonergic influences on amphetamine-induced stereotyped behavior.


Acetylcholine and cortical excitation by pyramidal tract collaterals.


Observations on bluetongue and epizootic hemorrhagic disease viruses in white-tailed deer: (1) distribution of virus in the blood (2) cross-challenge.


Mesodermal mixed tumors of the uterine corpus.


Pituitary-adrenal hormones and extinction of rewarded behaviour in the rat.


The effect of large doses of medroxyprogesterone acetate (MPA) on urinary estrogen levels and serum levels of cortisol T4 LH and testosterone in patients with advanced cancer.


Neurologic complications of corticosteroid therapy.


The adsorption of water in a chlorophyll extract.


Increased serum conjugated bilirubin in hemolytic anemia.


Female steroid hormones and target cell nuclei. The data discussed herein demonstrate the great variation in target-tissue response that can occur after administration of steroid hormones. The female sex steroids can exert regulatory effects on the synthesis, activity, and possibly even the degradation of tissue enzymes and structural proteins. Each response, nevertheless, appears to be dependent on the synthesis of nuclear RNA. In many instances, the steroid actually promotes a qualitative change in the base composition and sequence of the RNA synthesized by the target cell, implying a specific effect on gene transcription. Most important is our direct quantitative evidence that sex steroids cause a net increase in the intracellular amounts of specific mRNA molecules in target tissues. It thus appears that we are discovering a pattern of steroid hormone action which includes (Fig. 1): (i) uptake of the hormone by the target cell and binding to a specific cytoplasmic receptor protein; (ii) transport of the steroid-receptor complex to the nucleus; (iii) binding of this "active" complex to specific "acceptor" sites on the genome (chromatin DNA and acidic protein); (iv) activation of the transcriptional apparatus resulting in the appearance of new RNA species which includes specific mRNA's; (v) transport of the hormone-induced RNA to the cytoplasm resulting in synthesis of new proteins on cytoplasmic ribosomes; and (vi) the occurrence of the specific steroid-mediated "functional response" characteristic of that particular target tissue. To elucidate fully the mechanism of steroid hormone action we must study the biochemistry of the process by which information held by the steroid hormone-receptor complex is transferred to the nuclear transcription apparatus. If our assumptions are correct, we should ultimately be able to discover how this hormone-receptor complex exerts a specific regulatory effect on nuclear RNA metabolism. Such regulation might be achieved (i) by direct effects on chromatin template leading to increased gene transcription and thus RNA synthesis; (ii) by activation of the polymerase complex itself; (iii) by inhibition of RNA breakdown; or (iv) by intranuclear processing of large precursor molecules so that smaller biologically active sequences are produced, and (v) by transport of RNA from the nucleus to the cytoplasmic sites of cellular protein synthesis.


Failure to confirm cyclic AMP as second messenger for norepinephrine in rat cerebellum. Microiontophoretic applications of adenosine 3',5'-monophosphate (cyclic AMP) to spontaneously active, electrophysiologically identified Purkinje cells of the rat cerebellum failed to mimic the strong depressant action of norepinephrine on the same cells. These findings, in combination with a reevaluation of other studies, cast doubt on the hypothesis that cyclic AMP mediates the depressant actions of norepinephrine in the cerebellum.


Viscosity of cellular protoplasm.

Viscosity of cellular protoplasm. The protoplasmic viscosity was studied by using a small spin label having high permeability and broad solubility properties and nickel chloride as an extracellular spin-subtracting agent to localize signal inside cells. The viscosity is variable and in some cells is many times that of water or phospholipids, suggesting that lateral diffusion in biological membranes is important to cell function.


Diphenylhydantoin: effects on calcium metabolism in the chick. Rickets, hypocalcemia, decreased duodenal calcium transport, and reduction of calcium binding protein have been produced in chicks treated with diphenylhydantoin. These effects are directly related to diphenylhydantoin dose and inversely related to the intake of vitamin D(3) (cholecalciferol).


Origin of the mitochondrial RNA of animal tissues. 2. Hybridization with nuclear DNA.


Observations on the role of viruses in pneumonia of calves.


The molecular organization of the active sites of the microsomal cytochrome P-450.


The IBR-IPV virus-serum neutralization test. Sensitivity and significance of the tissue culture tube test.


Proteases of Clostridium botulinum. V. Studies on the serological relationship between proteases from Clostridium botulinum and other spore-forming bacteria.


Primary congenital eversion of the eyelids.


Conclusions from Bell's phenomenon variants.


Peripheral plasma levels of corticosteroids in normal beagles and greyhounds measured by a rapid competitive protein binding technique.


Stein-Leventhal syndrome in a 12-year-old girl.


Epstein-Barr virus antibodies in the cerebrospinal fluid. A case of infectious mononucleosis with encephalitis.


Galactography: a method of detection of unsuspected cancers.


Comparison of counterimmunoelectrophoresis with other serologic tests in the diagnosis of amebiasis.


Prolongation of a pancuronium-induced neuronmuscular blockade by polymyxin B.


Isolated facial numbness. Clinical significance and relation to trigeminal neuropathy.


Endoscopic cholangiography for biliary system diagnosis.


Studies on bone matrix glycoproteins. Incorporation of (1-14C)glucosamine and plasma (14C)glycoprotein into rabbit cortical bone. The radioactively labelled constituents present in bone matrix were compared 12 days after injection of either [(14)C]glucosamine or plasma [(14)C]glycoprotein. Both precursors are utilized in the synthesis of organic matrix by bone tissue. Cortical bone from animals injected with [(14)C]glucosamine contains radioactivity derived from glucosamine and plasma glycoproteins and all glycoprotein fractions are labelled. Plasma [(14)C]glycoprotein labels the less acidic glycoproteins to a greater extent than the more acidic components. An antibody has been raised against the less-acidic-glycoprotein fraction of bone. The latter contains a glycoprotein of alpha-mobility that appears to be concentrated specifically in bone tissue and which is present also in plasma. This alpha-glycoprotein accounts for a large proportion of the components labelled and retained in bone matrix after [(14)C]glucosamine injection.