Digestive physiology with reference to enzymes in the alimentary canal of coridius janus Fabr. (Heteroptera: Dinidoridae).


The lung in relation to vasoactive hormones.


Hormone: receptor interactions. Biological activities of (phenylalanine, norvaline)-adrenocorticotropin-(1-24)-tetrakosipeptide and its 4,5-dehydro-4,5-ditritio-norvaline analogue in isolated rat lipocytes and adrenal cortex cells: lipolysis, corticosterone and cyclic adenosine-3',5'-monophosphate production.


Observations in an aftercare group.

Observations in an aftercare group.


Simultaneous quantitative comparison of the optical changes at 700 nm (p700) and electron spin resonance signals in system I of green plant photosynthesis.


Myasthenia gravis.

Myasthenia gravis.


Chloroethyl-cyclohexyl-nitrosourea (CCNU) in the treatment of malignant brain tumors.


Postoperative intracavitary chemotherapy of malignant gliomas. A preliminary study using methotrexate.


Ventriculoatrial shunt blockage by previous positive contrast ventriculography.


Relationship of end-plate morphology to junctional chemosensitivity.


Analysis of Mauthner cell responses to iontophoretically delivered pulses of GABA, glycine and L-glutamate. 1. The intracellularly recorded responses of goldfish Mauthner neurones to iontophoretically applied pulses of amino acids have been analysed: their time courses have been compared with each other, and with those predicted from diffusion theory.2. The rise time of the response to GABA is slower than that to glycine or L-glutamate. The response curves of the latter substances were very similar, and unlike that of GABA were markedly affected by increasing the distance of pipette-tip from the membrane. The results suggest that the time course of the responses to glycine and L-glutamate are determined mainly by free diffusion in the brain tissue (at least within about 200 mum of the cell), while that to GABA must be rate-limited by other factors, e.g. drug-receptor activation time.3. The possibility that the responses are influenced by some desensitizing process was investigated by applying a second (test) drug pulse during the response to a prior conditioning one. In the case of glycine and of L-glutamate there was no attenuation of the response to a second pulse at any time. With GABA, however, the second response was reduced during the period of the conditioning response; the reduction was progressively less marked the later the test pulse occurred. A similar effect with GABA was seen when glycine was used as the test pulse. The responses to long-maintained drug pulses also indicated that for GABA, but not for glycine or glutamate, there seems to be some desensitizing process present.4. Calculated time courses of responses to brief pulses of glycine and of L-glutamate (based upon diffusion theory) differed somewhat from the observed curves, largely during the falling phase. However, when the calculations were based upon second-order reactions (two molecules of drug per receptor) the diffusion model gave results very like the observed ones.5. Possible implications of these results for the role these three amino acids may have as neuro-transmitters are mentioned.


Absorption of fortification iron by the rat: comparison of type and level of iron incorporated into mixed grain cereal.


The effect of nicotinic acid and alloxan on the secretion of electrolytes and amylase by the isolated saline perfused pancreas of the cat.


Spontaneous quantal transmitter release: a statistical analysis and some implications. 1. Miniature end-plate potentials (m.e.p.p.s) were intra- and extracellularly recorded from neuromuscular junctions in rat phrenic nerve-diaphragm preparations in vitro.2. Statistical analysis of the intervals between m.e.p.p.s showed that when the mean number of events in time t was plotted as a function of the variance of the events in time t there was a significant deviation from the straight line relationship expected for a Poisson process. Computer simulation showed that this deviation is explicable if release was generated by the random phasing of the activity of a number of releasing sites.3. There was no indication that release of one quantum influences the probability of release of remaining quanta (drag, clustering). It is suggested that m.e.p.p.s whose amplitude is larger than the mode result from the release of the contents of vesicles whose volume is also supramodal.4. The effects of depolarization of nerve terminals upon the variance-mean curve suggest an increase in the activity of sites rather than an increase in their number.5. Statistical analysis indicated at least 200 +/- 100 (mean +/- 1 S.E.) releasing sites. This number is of the same order as the number of sites of vesicle aggregation and presynaptic membrane density seen in electron micrographs of nerve terminals of this preparation.


The membrane effects, and sensitivity to strychnine, of neural inhibition of the Mauthner cell, and its inhibition by glycine and GABA. 1. Anionic conductance changes in Mauthner neurones of goldfish were measured during synaptically evoked inhibition and inhibition caused by iontophoretic application of the putative inhibitory transmitters glycine and gamma-aminobutyric acid (GABA).2. The effects of either amino acid were indistinguishable from those of the neural inhibitory transmitter(s). The membrane permeability during the neural or drug response was increased to Br(-), Cl(-), I(-), SCN(-), NO(3) (-), ClO(3) (-), and formate (HCOO(-)), but not to HCO(3) (-), BrO(3) (-), IO(3) (-), SO(4) (-), HPO(4) (-), H(2)PO(4) (-), acetate and citrate.3. Strychnine was injected intramuscularly, iontophoretically, or applied topically to the exposed brain in order to compare quantitatively its ability to prevent inhibition evoked by synaptic activation and by pharmacological means. Inhibitions were measured by the increase in membrane conductance.4. Strychnine, at concentrations just adequate to block completely the late collateral inhibition (LCI) and crossed VIII nerve inhibition, had little effect on the pharmacological inhibition caused by glycine, and sometimes there was no detectable effect at all. In one experiment even a local iontophoretic application of strychnine in a sufficient dose to diffuse over the cell and block the LCI almost completely, merely halved the effect of a small dose of glycine applied to the same localized region of the membrane.5. Higher concentrations of strychnine than those necessary to block synaptically evoked inhibition would reduce the effect of glycine but not that of GABA. The evidence indicated that any apparent effect of strychnine upon GABA could be explained by displacement of the GABA-containing iontophoretic pipette.6. The glycine-blocking action of iontophoretic pulses of strychnine was of relatively very slow onset and long duration compared to the effects of pulses of glycine and GABA.7. These findings can be interpreted as either (1) strychnine has a presynaptic action, preventing the release of inhibitory neurotransmitter, in addition to its less potent post-synaptic one in blocking pharmacological inhibition, or (2) strychnine acts entirely post-synaptically, but the physiological transmitter action differs from that of glycine and GABA in being considerably more sensitive to strychnine antagonism. In either case, the use of strychnine as evidence for the claim that glycine is an inhibitory neurotransmitter at the Mauthner cell is questionable.


Correlation of the clinical response to ACTH treatment with the endocrine status in myasthenia gravis.


Small cell carcinoma of the lung. Clinicopathological studies.


Primary mucinous adenocarcinoma of the urinary bladder.


Buoyant densities of mitochondrial DNA from tumour and normal tissues.


Beclomethasone aerosol in childhood asthma.


Control of fetal lung development in the rabbit.


Beclomethasone dipropionate aerosol in childhood asthma.


Effect of human growth hormone treatment on adipose tissue in children.


Serological studies in asymptomatic congenital cytomegalovirus infection.


Action of propranolol on lipolysis induced by catecholamine, dibutyryl cyclic AMP and serum.


Effect of intracellular accumulation of inert carbon particles on the cytochromes P-450 and b5 levels of rat liver microsomes.


Effects of sodium salicylate and acetylsalicylic acid on cyclic 3',5'-AMP-dependent protein kinase.


Cytochrome c stimulated oxidation of ethanol by liver mitochondria.


Selective inhibition of nicotinamide adenine dinucleotide dependent oxidations by piperazinothioureas.


Comparative study of cytochromes between virus-transformed and untransformed cells.


Studies on the activation of purified mitochondrial ATPase by phospholipids.


Energy conservation capacity and morphological integrity of mitochondria in hypotonically treated rabbit epididymal spermatozoa.


Low-temperature spectral properties of the respiratory chain cytochromes of mitochondria from Crithidia fasciculata.


Oxidative phosphorylation and effects of aerobic conditions on Rhodopseudomonas viridis.


Cyanide-resistant respiration and a branched cytochrome system in Kinetoplastidae.


Pigment content and molar extinction coefficients of photochemical reaction centers from Rhodopseudomonas spheroides.


The oxidation-reduction potential of membrane-bound chloroplast plastocyanin and cytochrome f.


Evidence for the identity of P430 of Photosystem I and chloroplast-bound iron-sulfur protein.


Purine nucleotide biosynthesis in gastrointestinal mucosa.


Anion-exchange chromatography on reversed-phase columns: isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasm.


Characterization of angiotensin receptors in vascular and intestinal smooth muscles. 1. A series of analogues of angiotensin II (AT(II)) has been used in the present experiments to characterize receptors for AT(II) in intestinal (rat stomach strip, rat colon) and vascular (rabbit aorta) smooth muscles. Two types of compounds have been chosen: (a) agonists with reduced potency, in which 4-Tyr, 6-His or 7-Pro had been substituted with L-Ala or with Gly and 1-amino-cyclopentane carboxylic acid (Acpc) and (b) competitive antagonists (8-Gly-AT(II), 8-Leu-AT(II)).2. Replacement of 4-Tyr, 6-His and 7-Pro with L-Ala decreases the potency, but does not influence the maximum effect of the analogue, while substitution of the same residue with Gly and Acpc reduces both potency and maximum effect.3. Compounds showing full size maximum responses were chosen to establish the following order of potency on the three preparations: AT(II)>4-Phe-AT(II)>7-Ala-AT(II)>6-Ala-AT(II)>4-Ala-AT(II).4. The four derivatives of AT(II) were completely inactive on tissues desensitized with AT(II). The responses to 5-hydroxytryptamine, acetylcholine and noradrenaline were not significantly modified, except for the rat colon.5. pA(2) values for the two competitive antagonists against AT(II) and 4-Phe-AT(II) were estimated in the three preparations by the use of the cascade superfusion technique. For comparison, pA(2) values were also estimated in rat stomach strips and rabbit aortas suspended in a normal organ bath, according to the method of Schild (1947). The similarities of the pA(2) values obtained in two series of experiments indicate that (a) the cascade superfusion technique is suitable for this type of study and (b) the receptor for AT(II) in the three tissues may be the same.6. It is suggested that receptors for AT(II) in intestinal and vascular smooth muscles may be the same, because (a) the order of potency of various agonists follows the same pattern, (b) the agonists are inactive on tissues desensitized with AT(II), (c) pA(2) values for competitive antagonists are similar in the three preparations.


Interaction between picrotoxin and 5-hydroxytryptamine in the superior cervical ganglion of the cat. 1. Electrophysiological techniques were utilized to study the actions of 5-hydroxytryptamine (5-HT) and picrotoxin on the superior cervical ganglion of the cat.2. The intra-arterial administration of 5-HT to the ganglion elicited both depressant and excitatory actions. In low doses (0.01-0.5 mug) the amine produced a depression of ganglionic transmission. In larger doses (2-50 mug) it produced an excitation of ganglion cells (early discharge) and an initial enhancement of transmission, which was followed by depression. Picrotoxin (25-500 mug, i.a.) blocked the initial excitatory effects of 5-HT but did not block the depression. Picrotoxin did not antagonize the excitatory actions of injected cholinomimetic agents or potassium chloride.3. In ganglia conditioned by repetitive stimulation of the preganglionic nerve (30 Hz for 30 s) 5-HT also elicited a late-occurring and very prolonged discharge on certain postganglionic nerves (;spinal') but not on others (external carotid). The late discharge was only partially depressed by picrotoxin.4. Recordings from the surface of the superior cervical ganglion revealed that 5-HT produced three types of ganglionic potentials: (1) an initial transient depolarization which coincided with the early discharge, (2) a late-occurring, prolonged depolarization which coincided with the late discharge, and (3) a hyperpolarization which in some experiments accompanied the depression of transmission. The late depolarization and hyperpolarization were not observed in every experiment. Picrotoxin (25-500 mug) blocked the initial depolarization, but did not block the late depolarization or the hyperpolarization.5. It is concluded the 5-HT can produce three distinct responses in the superior cervical ganglion: a depressant effect and two types of excitation. It seems likely that depression and excitation occur via the activation of different receptors, since picrotoxin selectively blocks the latter. The finding that picrotoxin is a 5-HT antagonist in peripheral ganglia raises the possibility that picrotoxin might also influence tryptaminergic mechanisms in the central nervous system.


The effect of glibenclamide on two enzymes important in gluconeogenesis.


Potentiation by angiotensin II of noradrenaline-induced contractions of a rabbit isolated thoracic aorta.


Atropine sensitivity of transmission and facilitation in the rabbit superior cervical ganglion.


Drugs influencing plasma and brain tryptophan.


Plasma renin and the antihypertensive effect of 1-Sar-8-Ala-angiotensin II.


In vivo changes in the concentration of cerebral cyclic AMP, phosphorylase a and glycogen induced by biogenic amines.


Effect of propranolol and theophylline on renin release caused by furosemide in the cat.


Inhibition by SQ 20881 of vasopressor response to angiotensin I in conscious animals.


The effects of theophylline and cyclic AMP on intestinal smooth muscle contractions.


Behaviour of enzymes at high concentration. Use of permeabilised cells in the study of enzyme activity and its regulation.


In vitro inhibition by synthetic phospholipids of pressor responses to renin.


Stereochemistry of the hydrogen transfer to the coenzyme by octopine dehydrogenase.


Enzyme kinetic parameters of the fluorescent ATP analogue, 2-aminopurine triphosphate.


Histone phosphorylation in the regenerating rat liver independent of diurnal rhythmicity.


Identification of 5-fluorocytidine in RNA from Escherichia coli grown in the presence of 5-fluorouracil.


Phosphorylase phosphatase: molecular configuration and activity.


Effects of long-acting thyroid stimulator on the reorganization into follicles of isolated thyroid cells and on the binding of radioiodinated thyrotropin to reassociated cells.


The superoxide dismutase activity of human erythrocuprein.


Glutathione reductase and glutathione peroxidase activities in hepatomous livers of rats treated with diethylnitrosamine.


Carbohydrate composition and sequence analysis of cell surface components by mass spectrometry. Characterization of the major monosialoganglioside of brain.


Cytochrome c linked nicotinic acid hydroxylase in Pseudomonas ovalis Chester.


Estrogen receptor: loss of DNA binding ability following trypsin or Ca2+ treatment.


Preparation of antisera to alpha-fetoprotein making use of estradiol affinity column.


Electron microscopic examination of hepatic subcellular fractions for phosphatases.


The production of IgE and IgG1 antibodies in guinea-pigs immunized with antigen and bacterial lipopolysaccharides.


Stimulation of transplantation immunity and plasma cell reaction by interferon in mice.


The redox behavior of metallo octaethylporphyrins.


Guinea-pig reaginic antibody. II. Physicochemical and biological properties.


Structures of analogs of nicotinamide-adenine dinucleotide. II. Crystal structure of N-(3-(Aden-9-yl) propyl)-3-carbamoylpyridinium bromide hydrobromide dihydrate, (AdeH plus-C3-Nic+)2Br-2H2O.


Structure of analogs of nicotinamide-adenine dinucleotide. I. Crystal structure of N-(3-(aden-9-yl)propyl)-3-carbamoylpyridinium bromide trihydrate, (Ade-C3-Nic+)Br-3H2O.


Structures of analogs of nicotinamide-adenine dinucleotide. 3. Crystal structure of 1,1'-trimethylenebisnicotinamide dichloride monohydrate, (Nic plus-C3-Nic+)2CL-H2O.


Chemical evolution of a nitrogenase model. VI. The reduction of CN-, N3-, N2O, N2, and other substrates by molybdocysteine catalysts in the presence of nucleoside phosphates.


Transmethylation from toxoflavines to nucleophiles.


Rotamer stability of histidine and histidine derivatives.


Spectral characteristics of yeast cytochrome c's at the temperature of liquid nitrogen.


Carboxamidomethylation of yeast inorganic pyrophosphatase.


Effect of carboxyl group modification on some of the enzymatic properties of L-glutamate dehydrogenase.


High-pressure liquid chromatography of modified purine mononucleotides and nucleosides.


The analysis of estrogenic steroids in urine by high-speed liquid chromatography.


Odontogenic myxoma: ultrastructural and histochemical studies. An odontogenic myxoma of a maxilla has been examined. Histochemistry of the mucosubstance indicated that hyaluronic acid and chondroitin sulphate were present. On ultrastructural examination many of the cells in the myxomatous tissue were seen with prominent rough endoplasmic reticulum, suggesting a secretory function, and possibly the myxomatous ground substance was produced by these cells. Cells containing collagen fibrils were found. Epithelial islands with intercellular spaces producing an arrangement similar to the stellate reticulum of the enamel organ were found, and possibly had developed from odontogenic epithelial rests. These islands were surrounded by a clear zone, outside which were cells with an increased prominence of adenosine-triphosphatase, nucleoside-diphosphatase, thiamine-pyrophosphatase, and often arylamidase reaction products. This probably represents a reaction of the mesodermal tissue to the epithelial islands. Possibly the epithelium exerts an inductive effect on the mesodermal tissue, and the myxomatous appearance may be the result of an aberrant development of mesodermal cells into ;myxoblasts' which secrete the myxomatous ground substance.


Phospholipids and their metabolism.

Phospholipids and their metabolism.


Lipid methodology.

Lipid methodology.


Cholesterol metabolism.

Cholesterol metabolism.


Classification of lipoproteins and lipoprotein disorders.


Lipoprotein fractionation.

Lipoprotein fractionation.


Plasma triglyceride metabolism.

Plasma triglyceride metabolism.


Benzyl 6-oxopenicillanate and derivatives. II.


Electron carriers of cytochrome oxidase detectable by electron paramagnetic resonance and their relationship to those traditionally recognized in this enzyme. On the basis of oxidoreductive rapid kinetic and titration experiments with purified cytochrome c oxidase (EC 1.9.3.1), monitored by electron paramagnetic resonance (EPR) at 13 degrees K and by spectrophotometry at 100 degrees K, a new assignment of EPR signals is proposed. The bulk of both the low-spin (g = 3.0; 2.2; 1.5) and highspin (g = 6; 2) signals is attributed to the component with the properties of traditional cytochrome a. It is further proposed that the absorption band at 655 nm represents the most unambiguous manifestation of the a(3) component.


Independent segregation of the H-2 locus and the locus for responsiveness to histamine-sensitizing factor. The inheritance of responsiveness to histamine-sensitizing factor of pertussis bacilli was examined in (SWR/J x CBA/J)F(1) x CBA/J backcross mice. We found that about half of the backcross mice inherited this responsiveness in both sexes. There was no linkage between the inheritance of responsiveness to histamine-sensitizing factor and the H-2(q) locus from the sensitive SW/J progenitor.


Malignant lymphoma in cottontop marmosets after inoculation with Epstein-Barr virus. Neoplasia resembling human malignant lymphoma, reticulum cell sarcoma type, occurred in cottontop marmosets inoculated with materials containing Epstein-Barr virus. One of four monkeys that received autologous cells transformed in vitro by Epstein-Barr virus developed lymphoma in mesenteric lymph nodes 7.5 months after inoculation. Three of four marmosets inoculated with cell-free Epstein-Barr virus developed lymphoma. The latent period for detectable tumor formation after addition of virus was 31-46 days. Immunosuppressive drugs given with the virus accelerated the course of disease. Nevertheless, malignant lymphoma occurred in an animal given only cell-free virus. Six of eight marmosets inoculated with the virus demonstrated antibodies to the virus. Four marmosets not exposed to the virus, including two that received immunosuppressive drugs, developed neither tumors nor antibodies to Epstein-Barr virus. Virus antigen detectable by immunofluorescence was found in 5% of cells shed from one tumor maintained in organ culture. These results imply that Epstein-Barr virus is capable of inducing malignant lymphoma in at least one primate species. Additional evidence is required before its oncogenic capacity in this host can be accepted without reservation.


Cyclic adenosine monophosphate receptor: loss of cAMP-dependent DNA binding activity after proteolysis in the presence of cyclic adenosine monophosphate. The cAMP receptor requires cAMP for DNA binding at pH 8.0 but shows cAMP-independent DNA binding at pH 6.0. Incubation of the cAMP receptor with proteolytic enzymes in the presence of cAMP results in loss of DNA-binding ability at pH 8, while it is still able to bind cAMP and DNA at pH 6. Incubation with proteolytic enzyme in the absence of cAMP does not affect the DNA-binding properties of the cAMP receptor. After proteolysis in the presence of cAMP, analysis by sodium dodecyl sulfateacrylamide-gel electrophoresis shows that the 22,500-dalton subunit characteristic of the untreated protein has been completely replaced by a 12,500-dalton fragment.


An immobilized three-enzyme system: a model for microenvironmental compartmentation in mitochondria. An immobilized three-enzyme system, malate dehydrogenase (EC 1.1.1.37)-citrate synthase (EC 4.1.3.7)-lactate dehydrogenase (EC 1.1.1.27), was investigated as a model for the rate of oxalacetate production and utilization in mitochondria. Lactate dehydrogenase is included to mimic the NADH-utilizing system of mitochondria. This three-enzyme system was immobilized in three different ways (1) on Sephadex G-50 (surface coupling), (2) on Sepharose 4B (internal-external coupling), and (3) entrapped in polycrylamide gel. The rate of citrate production from malate, NAD(+), and acetyl CoA was determined continuously in a flow system. Up to about 100% rate enhancements were observed when the immobilized system was compared to identical systems of free enzyme. An even more pronounced increase of rate of up to about 400% compared to the soluble system was measured after addition of pyruvate (to reoxidize formed NADH). These results are interpreted in relation to microenvironmental changes of oxalacetate production and the possible organization of enzymes of the Krebs cycle.


Host restriction of Friend leukemia virus. Role of the viral outer coat. Host restriction of oncogenesis of RNA tumor viruses in vivo is associated with several gene loci. One of these genes, the Fv-1 locus in mice, is expressed in vitro and may be studied in mouse-embryo cultures that are restrictive or permissive for replication of Friend leukemia virus. Two strains of Friend leukemia virus, N-or B-tropic, show reciprocal ability to replicate successfully in either NIH Swiss (N-type) or BALB/c (B-type) cells that differ at the Fv-1 locus. These two strains of virus and two cell lines form a system to measure host restriction in vitro. Measurement of adsorption of Friend leukemia virus to permissive or restrictive cells reveals no difference in rate or total amount of virus bound. Furthermore, studies with virions of vesicular stomatitis virus phenotypically mixed within an envelope containing Friend leukemia virus protein show no differences in penetration or replication of vesicular stomatitis virus. These results strongly suggest that host restriction of Friend leukemia virus is due to an intracellular event in the viral replication cycle.


Reexpression of the rat hypoxanthine phosphoribosyltransferase gene in rat-human hybrids. Fusion of hypoxanthine phosphoribosyltransferase (HPRT)(-) rat hepatoma cells with HPRT(+) human fibroblasts yielded hybrid clones that grew in HAT selective medium and contained all the rat chromosomes and one to nine human chromosomes. Among the retained chromosomes was the human X chromosome. In all clones backselected in medium containing 8-azaguanine, human X chromosome was absent. Electrophoretic analysis revealed that, without exception, hybrid clones growing in HAT medium had an active HPRT enzyme, either human or rat, or both. When these clones were backselected in 8-azaguanine, they did not show HPRT enzyme activity. Hybrids that contained the human X chromosome also had human glucose-6-phosphate dehydrogenase. The observed reexpression of rat HPRT in hybrid cells derived from HPRT(-) rat cells suggests that a genetic factor from the human cell determined the expression of the rat structural gene for HPRT.


Replacement of the sequence G-T-phi-C-G(A)- by G-A-U-C-G- in initiator transfer RNA of rabbit-liver cytoplasm. Eukaryotic cytoplasmic initiator tRNAs lack the sequence G-T-Psi-C-G(A)-, which is in every tRNA of known sequence that is active in protein biosynthesis. In initiator tRNA of yeast cytoplasm, which is the only eukaryotic initiator tRNA of known sequence, this sequence is replaced by G-A-U-C-G-. We now report the sequence of a 30-nucleotide-long, 3'-terminal fragment of cytoplasmic initiator tRNA of rabbit liver obtained by specific cleavage of the tRNA at the site occupied by the modified nucleoside, 7-methyl guanosine. We show (i) that in rabbit-liver initiator tRNA also, the sequence G-T-Psi-C-G(A)- is replaced by G-A-U-C-G- and (ii) that the sequences of loop IV of both the yeast and rabbit-liver cytoplasmic initiator tRNAs are identical, A-U-C-G-m(1)-A-A-A-.


Pituitary extracts and steroid hormones in the control of 3T3 cell growth. Results are reported showing that pituitary extracts stimulate growth of 3T3 cells, an established line of mouse fibroblast. Cells were cultured in medium containing an organic buffer (HEPES; N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), which increases the serum requirement for cell growth. The pituitary growth-prompting activity is (a) not dialyzable; (b) thermolabile; (c) protease sensitive; and (d) enhanced by hydrocortisone (a steroid hormone). Activity was quantitatively assayed by measurement of stimulation of cell division or [(3)H]thymidine uptake into DNA. The active species is probably different from pituitary protein hormones and it seems to be effective at concentrations observed for protein hormones in vivo. 3T3 cells transformed by simian virus 40 are not responsive to this activity.


Enzyme loading of erythrocytes.

Enzyme loading of erythrocytes. We demonstrated that beta-glucosidase and beta-galactosidase can be trapped inside erythrocytes by rapid hemolysis of the cell in the presence of these enzymes. Enzyme enters only during hemolysis, and optimum uptake occurs within 60 sec. There is no loss in cell number after hemolysis-induced enzyme uptake, and the ghosts have only a slightly increased mean cell volume. Smaller proteins enter more readily than larger proteins, although enzymes with a molecular weight of at least 180,000 can be readily entrapped by erythrocytes. This finding may provide a useful approach to the problem of enzyme replacement in certain diseases, including Gaucher's disease.


Toward a comparative biochemistry of the cytochromes.


Production of hemorrhagic necrosis in guinea pigs with anti-streptococcal antisera.


Non-specific arthralgia and myalgia with subclinical neuropathy. A histochemical and histological study.


Selective secretion of lysosomal enzymes of the Yoshida hepatoma AH 130 in the peritoneal fluid.


Calcium binding to phospholipids from experimental tumors.


Pseudomonas cytochrome c peroxidase. IX. Molecular weight of the enzyme in dodecyl sulfate-polyacrylamide gel electrophoresis.


Calcification in adenocarcinoma.

Calcification in adenocarcinoma.


Ultrastructure of the obligately anaerobic bacteria Clostridium kluyveri and Cl. acetobutylicum.


Acute effects of actinomycin D on the binding of tritiated oestradiol by the mouse vagina.


The membrane ATPase of Escherichia coli. I. Release into solution, allotopic properties and reconstitution of membrane-bound ATPase.


Polycation inhibition of chloroplast photosystem I.


Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacter vinelandii.


On the triple localization of creatine kinase in heart and skeletal muscle cells of the rat: evidence for the existence of myofibrillar and mitochondrial isoenzymes.


Acylation of Escherichia coli tRNAtrp with 5-methyltryptophan by E. coli tryptophanyl-tRNA ligase.


Regulation of RNA synthesis in Escherichia coli. I. Characterization of cells subjected to simultaneous temperature and osmotic shock.


Evidence for de novo synthesis of collagenase by tadpole skin explant.


Superficial hydradenoma of the cheek.

Superficial hydradenoma of the cheek.


Activity of adenosine 3',5'-cyclic monophosphate phosphodiesterase in tissues of adult locust Schistocerca gregaria L. (Orthoptera).


The lipid core model of lipoproteins.

The lipid core model of lipoproteins.


Effect of aldosterone on cyclic-AMP metabolism in the urinary bladder of the toad.


Cyclic-AMP in the aqueous humor: the effects of repeated topical epinephrine administration and sympathetic denervation.


Cyclic nucleotides and photoreception.

Cyclic nucleotides and photoreception.


Cytotoxic compounds. XVI. Reactions of the bismethanesulphonates of 3-(N-methylanilino)propane-1,2-diol and of 2-(N-methylanilino)-propane-1,3-diol with nucleophiles.


Isoindole.

Isoindole.


Sizes of end plate compartments, densities of acetylcholine receptor and other quantitative aspects of neuromuscular transmission.


Cellular events in tolerance. I. Failure to demonstrate activation of lymphocytes, blocking factors, or suppressor cells during the induction of tolerance to a soluble protein.


Histological and ultrastructural changes in the blastocyst and reproductive tract of the roe deer, Capreolus capreolus, during delayed implantation.


A re-examination of the rôle of phospholipids as energy substrates during incubation of ram spermatozoa.


Hormone receptors.

Hormone receptors.


A mutant of Escherichia coli K12 deficient in the 5'-3' exonucleolytic activity of DNA polymerase I. I. General characterization.


Pollicization for congenital deformities of the hand.


Associated carcinomata of colon and anus.


Radiographic appearance of bronchial carcinoid.


Calcitonin- and ACTH-producing cells in a case of medullary carcinoma of the thyroid. Immunofluorescence investigations.


Liver necrosis, adenovirus type 2 and thymic dysplasia.


RNAs as amplifiers of specific signals in immunity.


Various enzymes involved with putative neurotransmitters. Regional distribution in the brain of deceased mentally normal, chronic schizophrenics or organic brain syndrome patients.


Electron microscopy of selectively stimulated microconidiogenesis in wild type Neurospora crassa.


Structure and plugging of septa of wild type and spreading colonial mutants of Neurospora crassa.


The regulation of liver glycogen synthetase D phosphatase by ATP and glucose.


A preliminary immunochemical study of E. coli aspartokinase I-homoserine dehydrogenase I.


Modification of polynucleotides by a fragment produced by enzymatic cleavage of S-(1, 2-dichlorovinyl)-L-cysteine.


Subunits of oncornavirus high-molecular-weight RNA. I. Stepwise conversion of 60 S AMV (avian myeloblastosis virus) RNA to subunits.


Binding of progesterone by rat uterus in vitro.


Low temperature electron paramagnetic resonance studies on two iron-sulfur centers in cardiac succinate dehydrogenase.


13C nuclear magnetic resonance studies of the conformation of the X-pro bond in the oligopeptide hormones, thyrotropin-releasing hormone, luteinizing hormone-releasing factor, angiotensin and melanocyte-stimulating hormone release-inhibiting factor.


Alanine formation by rat muscle homogenate.


Alternate pathways of D-fructose transport and metabolism in Arthrobacter pyridinolis.


Adenosine 3', 5'-monophosphate-dependent protein kinase in adipose tissue: inhibition by tolbutamide.


Conversion of DNA polymerase extracted from rat ascites hepatoma cells.


Adenosine 3',5' monophosphate binds only to the inner surface of human erythrocyte membranes.


Effect of cytochalasin B on lymphocyte stimulation induced by concanavalin A or periodate.


A spin-label for RNA study.

A spin-label for RNA study.


Binding of thyrotropin releasing hormone by thyrotropin-secreting cells.


The molecular weight of NADPH-cytochrome C reductase isolated by immunoprecipitation from detergent-solubilized rat liver microsomes.


Gluconate metabolism in Pseudomonas: a novel pathway of glyceraldehyde-3-phosphate metabolism.


Cyclic nucleotide-dependent protein kinases of the rat pancreas.


Control of calcium absorption and intestinal calcium-binding protein synthesis.


Studies on ATP-dependent deoxyribonuclease of Haemophilus influenzae: involvement of the enzyme in the transformation process.


Synthesis in vitro of vesicular stomatitis virus proteins in cytoplasmic extracts of L cells.


Protein kinase activity in Morris hepatomas.


Role of 3',5'-cyclic AMP in the control of nuclear protein kinase activity.


Endonuclease of high specific activity in a purified mouse interferon preparation.


The stability of collagen cross-links when derived from hydroxylsyl residues.


Effect of catecholamines and ovarian hormones on cyclic AMP accumulation in rat hypothalamus.


Formation of retinol (alpha, beta-32P) pyrophosphate with (gamma-32P) ATP catalysed by whole homogenates of rat thyroid.


An iron tetrahydroporphyrin prosthetic group common to both assimilatory and dissimilatory sulfite reductases.


Similar and opposite effects of dibutyryl cyclic AMP and insulin on glucose metabolism in adipose tissue.


Effects of thyrotropin, prostaglandin E1 and iodide on cyclic 3',5'-AMP concentration in dog thyroid slices.


Development of cyclic AMP metabolism in rat liver. A correlative study of tissue levels of cyclic AMP, accumulation of cyclic AMP in slices, adenylate cyclase activity and cyclic nucleotide phosphodiesterase activity.


The role of calcium in the steroidogenic response of rat adrenal cells to adrenocorticotropic hormone.


Gonadotropin receptors in the plasma membranes of rat luteal cells.


Enzyme cytochemical observations on the tissue stages of the life cycle of Eimeria acervulina and Eimeria necatrix.


Changes in oxidative metabolism and ultrastructure accompanying differentiation of the mitochondrion in Trypanosoma brucei.


Oat cell carcinoma of the trachea.

Oat cell carcinoma of the trachea.


Paracrystalline arrays in mitochondria following ageing of mitochondria in situ.


Effect of the local administration of proteolytic enzymes on the rupture of the ovarian follicle in the domestic fowl, Gallus domesticus.


Phospholipid composition of bovine sperm fractions, seminal plasma and cytoplasmic droplets.


Effect of morphine administration on adenyl cyclase and 3',5'-cyclic nucleotide phosphodiesterase activities in the brain.


Metabolism of fatty acids by ovine spermatozoa.


Modification of Baird-Parker's classification system of Staphylococcus albus.


The detection of different progenies induced by fractionated infectious poliovirus RNA.


Latent infection of monkeys with oncogenic herpes viruses.


Simple preparation of 3H-labelled poliovirus for chromatographic strain differentiation with aluminium hydroxide and one-year control of labelled reference strains.


The role of lysosomes in virus-induced cell fusion. 2. Modification of the cell surface.


The role of lysosomes in virus-induced cell fusion. 1. Cytochemical studies.


A study of the conformational properties of hemoglobin by the method of two paramagnetic labels.


Some properties of cortisol-receptor complex isolated from cytoplasm of rat liver and its effect on biosynthesis of nuclear RNA.


Demonstration of a specific binding protein for testosterone and 5alpha-dihydrotestosterone in rabbit serum. Separation from the corticosteroid binding globulin (CBG).


In vitro effects of ACTH on uridine incorporation into rapidly labelled adrenal RNA of normal and dexamethasone-suppressed rats.


Estrogen receptor protein of pancreas.

Estrogen receptor protein of pancreas.


Water soluble proteins from rat brain and from experimental tumors of rat nervous system.


1-Nitroso-5, 6-dihydrouracil: induction of liver cell carcinomas and kidney adenomas in the rat.


Epidemiology of respiratory syncytial virus infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection.


Adrenocortical function after major gastric surgery.


Alteration in liver mitochondrial metabolism in a patient with biliary obstruction due to liver carcinoma.


Granular cell myoblastoma of the breast.


Pentamidine isothionate levels in lungs, livers, and kidneys of rats after aerosol or intramuscular administration.


Bovine respiratory secretion as a two-phase system. Effect of parainfluenza-3 virus on the rheologic properties of the phases.


Pulmonary blastoma.

Pulmonary blastoma.


Persistent infection with parainfluenza type 3 virus in man.


The mechanism of inhibition by fluoride of fatty acid oxidation in uncoupled mitochondria.


Changes in the lipid and fatty acid composition of developing rabbit brain.


Further characterization of fatty acyl monoester lipase from human adipose tissue.


Free and sulphate-conjugated 16-unsaturated C19 steroids in human testis tissue.


Tetrahydropteridine-dependent cleavage enzyme for O-alkyl lipids: substrate specificity.


5'-Nucleotide phosphodiesterase isoenzyme pattern in the serum of human hepatoma.


The acyl lipids of highly purified plant mitochondria.


Comparison of cell coat acid mucopolysaccharides of normal liver and various ascites hepatoma cells.


Guanosine anabolism for biosynthesis of nucleic acids in Novikoff ascites rat tumor cells in culture.


The effect of pH on the morphology, electrophoretic mobility, and infectivity of the mouse mammary tumor virus.


Virus morphogenesis in a mouse lymphoma.


Effects of the antineoplastic alkaloid acronycine on the ultrastructure and growth patterns of cultured cells.


Tissue culture and animal studies with an oncolytic bovine enterovirus (bovine enterovirus 1).


Incorporation of 2-deoxy-D-glucose into glucoproteins of normal and Simian virus 40-transformed hamster cells.


Purine nucleotide metabolism and nucleotide pool sizes in synchronized lymphoma L5178Y cells.


The protective effect of estradiol-17 beta against polycyclic hydrocarbon cytotoxicity.


Genetic factors in chronic remittent Friend disease.


Competitive DNA-RNA hybridization of nuclear and microsomal RNA in normal, neoplastic, and neonatal liver tissue.


Electron microscopic study of lymphatic tissues of mice inoculated with a leukemogenic extract from radiation-induced tumors.


Collagenolytic activity in malignant melanoma: physiocochemical studies.


Transfer RNA methylases and transfer RNA-methylated bases of polyoma virus-transformed and spontaneously transformed mouse culture cells.


Expression of tumor-specific antigens in mouse somatic cell hybrids.


Further observations on the activation and inhibition of lipoprotein lipase by apolipoproteins.


Mechanisms of activation of cardiac glycogen phosphorylase in ischemia and anoxia.


Contribution of intrarenal generation of prostaglandin to autoregulation of renal blood flow in the dog.


Differential response of large and small coronary arteries to nitroglycerin and angiotensin. Autoregulation and tachyphylaxis.


Improved techniques for assessment of serum lipoprotein patterns. II. Rapid method for diagnosis of type 3 hyperlipoproteinemia without ultracentrifugation.


Rapid determination of ammonia in a perchloric acid supernate from blood, by use of an ammonia-specific electrode.


Improved method for determination of triglycerides in serum.


Measurement of carcinoembryonic antigen.


Simian virus 40-induced retinopathy in the rat.


Contact sensitivity in vitro. The production of macrophage inhibition factors from DNCB sensitized lymphocytes by subcellular organelles obtained from DNCB epidermal tissue.


Increased sensitivity to theophylline associated with primary open-angle glaucoma.


Bluetongue virus in exotic ruminants.

Bluetongue virus in exotic ruminants.


An epornitic of duck virus enteritis (duck plague) in California.


Cheddar cheese as a vehicle for viruses.


Characterization of brain cerebrosides at early stages of development in the rat.


Stimulatory effect of L-vasopressin on PMSG-induced ovulation in immature rats.


Activity of mitochondrial enzymes in mouse embryos.


Congenital mesoblastic nephroma (leiomyomatous hamartoma): first adult case.


Granular cell myoblastoma of the bladder: report of an additional case.


Massive adrenal hemorrhage in the newborn: 2 surviving cases by surgical treatment.


Benign renal tumors in children: report of a case.


Retroperitoneal lipoblastic tumors in children.


Some observations on the pathogenesis of Bartter's syndrome.


Pseudohypoparathyroidism type II: a possible defect in the reception of the cyclic AMP signal.


Letter: Role of microtubules in lipoprotein secretion by the liver.


Prebiotic activation processes.

Prebiotic activation processes.


Letter: Ribonucleoprotein particle in epidermis cells as the receptor for juvenile hormone.


Peptide formation mediated by cyanate.

Peptide formation mediated by cyanate.


Guanidine hydrochloride in the Eaton-Lambert syndrome. Electrophysiologic improvement.


Diagnostic open lung biopsy in the critically ill child.


Bacterial meningitis. A symposium. 3. Morbid anatomical changes.


Distension dacryocystography.

Distension dacryocystography.


Debridement of experimental skin burns of pigs with bromelain, a pineapple-stem enzyme.


Idiopathic intestinal lymphangiectasis (primary protein-losing enteropathy). Lymphographic verification of enteric and peritoneal leakage of chyle.


99mTc-diphosphonate concentration in primary breast carcinoma.


Ultrasonic holography. A promising medical imaging tool.


Suppression of 99mTc-pertechnetate uptake in a choroid plexus papilloma.


Evaluation of 99mTc-pyrophosphate as a bone imaging agent.


Comparison of growth acceleration of pituitary dwarfs treated with anabolic steroid and thyroid hormone with normal growth spurt.


Paradoxical response of plasma cortisol to pulse intravenous injection of synthetic corticotrophin and dibutyryl cyclic adenosine 3',5'-monophosphate in adult panhypopituitarism.


"In vivo radioimmunoassay" of aldosterone.


The simultaneous assay of cortisol and 17alpha-hydroxyprogesterone in the plasma of patients with congenital adrenal hyperplasia.


The clinical pattern and prognosis of patients with amebic liver abscess and jaundice.


Studies on human conception.

Studies on human conception. In vitro and clinical research into the mechanisms of human conception is described.  The author discusses his own work and relevant literature in the field.  The points under study include the growth of follicles, the timing of ovulation, the biochemical changes in preovulatory follicles, fertilization and cell-splitting in vitro, and the endocrine control of implantation.  Results of the various clinical experimentation are graphed and tabulated.  It is hoped that such research will add to the accumulated knowledge of human physiology and help in the treatment of certain forms of human infertility.  It was found that mild hyperfolliculation could be achieved by use of hormonal preparations to induce follicular growth and ovulation.  Human menopausal gonadotropins were used to stimulate follicular growth, and human chorionic gonadotropin was used for inducing ovulation.  Follicles near the time of ovulation were expected to have low levels of estrogen and high levels of PGs (prostaglandins).  Assays of steroids in different follicular fluids revealed considerable variation in the levels of estrogen and progesterone.  PGs obviously play an important role in preovulatory follicle biochemistry.  In vitro methods for inducing fertilization and cleavage were also tried.  Areas for further study are outlined.


Social service casefinding in the hospital--its influence on the utilization of social services.


Streptozotocin for malignant insulinomas and carcinoid tumor. Report of eight cases and review of the literature.


Pituitary gigantism. A case report and review.


Editorial: Extra-articular lesions of rheumatoid arthritis.


Aberrant behavior of phosphofructokinase from otosclerotic stapedes. Biochemical evidence and the metabolic consequences.


Clinical experience with two new methanesulfonic acid esters of aminoglycols, NSC-140115 and NSC-140117, in the treatment of malignant lymphoma.


Circadian rhythm in the urinary excretion of cyclic 3',5'-adenosine monophosphate in man.


Estimation of ACTH reserve on normal and hypopituitary subjects: comparison of oral and intravenous metyrapone with insulin hypoglycemia.


Isolation of angiotensin II binding proteins from normal human plasma by affinity chromatography.


Release of transmitter from degenerating locust motorneurones.


Cell-associated nonesterified fatty acid levels and their alteration during lipolysis in the isolated mouse adipose cell. A rapid and flexible method has been developed for measuring cell-associated, probably intracellular, nonesterified fatty acids (CAFA) in isolated mouse adipose cells. A variety of lipolytic agents as well as various concentrations of epinephrine elevate CAFA levels in rough proportion to their stimulation of glycerol and fatty acid release. Insulin reduces epinephrine-elevated CAFA levels. A detailed, quantitative study of the relationship among lipolytic activity, CAFA levels, and the extracellular molar ratio of fatty acids to albumin has been carried out. Epinephrine-elevated CAFA levels rise linearly with, while epinephrine-stimulated lipolytic activity is independent of, fatty acid to albumin ratios below 2-3. As the ratio increases from 3 to 5, CAFA levels continue to increase, whereas lipolytic activity decreases. Above ratios of 5, fatty acid release almost completely ceases; CAFA levels increase dramatically with residual glycerol release. A temperature-dependent efflux of epinephrine-elevated CAFA can be elicited through blockade of stimulated lipolysis with propranolol, but only in the presence of extracellular fatty acid to albumin ratios below 3. These observations suggest that during stimulated lipolysis, a fatty acid gradient exists between the cell and extracellular serum albumin and that CAFA represent the intracellular component of this gradient. In addition, these observations support the concept that intracellular fatty acids play a role in the feedback regulation of adipose cell function as extracellular fatty acids accumulate during the lipolytic response.


Bimodal effect of insulin on hormone-stimulated lipolysis: relation to intracellular 3',5'-cyclic adenylic acid and free fatty acid levels. The present study was undertaken to determine the relationship between the antilipolytic and lipolytic effects of insulin on hormone-stimulated lipolysis and the mechanisms of these reactions. The dose-response curve of norepinephrine-stimulated lipolysis in rat adipocytes was not sigmoidal but biphasic in nature. Intracellular free fatty acid levels were linearly related to lipolytic rate and also described a biphasic profile in response to increments in norepinephrine concentration. Intracellular 3',5'-cyclic AMP levels measured 10 min after addition of increasing concentrations of norepinephrine showed a rise and a plateau followed by a secondary rise. Insulin was antilipolytic at low concentrations of norepinephrine and distinctly lipolytic at high concentrations. The combined antilipolytic and lipolytic effect of insulin is termed the "bimodal" effect of insulin on hormone-stimulated lipolysis. The bimodal effect of insulin correlated positively with changes in peak intracellular 3',5'-cyclic AMP levels. In the presence of glucose, insulin invariably enhanced lipolysis. It is suggested that the antilipolytic effect of insulin is achieved by both inhibition of adenyl cyclase activity and activation of low-K(m) 3',5'-cyclic AMP phosphodiesterase, the net effect being a low accumulation of 3',5'-cyclic AMP. On the other hand, the lipolytic effect of insulin probably reflects enhancement of adenyl cyclase activity to an extent that overrides any activation of low-K(m) 3',5'-cyclic AMP phosphodiesterase activity, resulting in an increase in peak adipocyte 3',5'-cyclic AMP levels.


Fatty acid oxidation and esterification in isolated rat hepatocytes: regulation by dibutyryl adenosine 3',5'-cyclic monophosphate. Isolated rat hepatocytes rapidly utilized [(14)C]palmitate and, in particular, synthesized large amounts of neutral lipids from palmitate. Incorporation into cellular lipids occurred at a linear rate proportional to the medium concentration of fatty acids. Oxidation of [(14)C]palmitate to CO(2) increased with time and was much slower than palmitate esterification. Since [(14)C]acetate and [(14)C]glucose were oxidized to CO(2) at a linear rate, the lag in fatty acid oxidation to CO(2) did not involve enzymatic steps subsequent to acetate formation. The relative contribution of palmitate to esterification and to CO(2) formation depended upon the molar ratio of palmitate to albumin (v) and the length of incubation. Dibutyryl cyclic AMP (1 mM) reduced the oxidation of palmitate and acetate to CO(2) by about 50 and 90%, respectively, but did not alter palmitate esterification. However, equivalent concentrations of sodium butyrate produced similar decreases in CO(2) formation. Dibutyryl cyclic AMP (1 mM) also stimulated palmitate oxidation to water-soluble products, principally ketone bodies, by 50-100%. Sodium butyrate exerted no effect, while monobutyryl cyclic AMP and cyclic AMP both stimulated this pathway significantly. These results indicate that both v and dibutyryl cyclic AMP regulate the metabolism of fatty acids by isolated hepatocytes and suggest that hormonal stimulation of adenyl cyclase controls hepatic lipid metabolism.


Letter: The role of antimicrobial agents in the treatment of pertussis.


Cell surface changes in virus-induced cell fusion. 2. Changes in cell coat thickness.


Cyclic AMP and cyclic GMP enhance growth of chick embryo fibroblasts.


Selective agglutination of pathogenic strains of Entamoeba histolytica induced con A.


Cushing's syndrome and pregnancy.

Cushing's syndrome and pregnancy.


Metastatic cancer of the pituitary gland.


Effects of ethanol on amino-acid uptake and utilization by the liver and other organs of rats.


Adenosine 3',5'-monophosphate metabolism in the liver in experimental hemorrhagic shock.


Spontaneous rupture of the liver caused by hepatomas.


Clinical liver heterotopic (auxiliary) transplantation.


5-Bromodeoxyuridine incorporation into simian virus 40 deoxyribonucleic acid. Effects on simian virus 40 replication in monkey cells.


Transformation of mammalian cells by DNA-containing viruses following photodynamic inactivation.


Crystallization of human rhinovirus 1A.

Crystallization of human rhinovirus 1A.


Formation of syncytia in human lymphoblastoid cells infected with type C viruses.


Establishment of repression by bacteriophage lambda: lack of a direct regulatory effect of cyclic AMP.


Presence of a thermosensitive step in the course of transformation by SE-polyoma virus.


The effect of cordycepin on cell transformation by RNA tumor viruses.


Effects of pH on the structure of vesicular stomatitis virus.


Polypeptide composition of measles and canine distemper viruses.


A national view of mental health.

A national view of mental health.


Phosphodiesterase inhibition and vascular activity of papaverine metabolites.


Analysis of the peristaltic reflex in vitro: effects of some antagonists.


Attempts to find a specific intranuclear location for replication polyoma virus DNA.


Intracellular distribution of steroid glycosidases of rabbit liver.


Letter: Comparing serologic data.

Letter: Comparing serologic data.


Toxicity, analysis, and production of aspergillic acid and its analogues.


A comparative study of the corneal graft reaction in allo- and xenogeneic models.


Editorial: New knowledge of benign essential hypertension.


Renal histology and clinical course of systemic lupus erythematosus. A prospective study.


Renin in hypertension: how important as a risk factor? An analysis of the plasma renin levels in relation to the incidence of severe cardiovascular complications (coronary thrombosis, stroke, ruptured aortic aneurysm) was made in 325 patients with various types of hypertension. These patients had one to four measurements of plasma renin activity taken under standard conditions of sodium intake and posture in the period 1963-68. The follow-up was 5 to 10 years in the four groups of hypertensive patients (essential hypertension, malignant hypertension, hypertension secondary to renal parenchymatous disease and hypertension caused by, or associated with, renal artery obstruction). For all 325 patients, the incidence of such complications was 23.6, 20.4 and 44.7% in the low, normal and high renin groups. These findings are at variance with the claim that renin constitutes a serious risk factor in hypertensive patients, especially if it is isolated from other parameters such as the level of diastolic pressure, the adequacy of kidney function, the effectiveness of dietary and drug management of hypertension, and especially the presence or absence of atherosclerotic lesions of the large vessels at the time of the renin determination.


Role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus. When cattle infected with infectious bovine rhinotracheitis virus (IBRV) were treated 3 months later with a synthetic corticosteroid, consistent recrudescence of the infection was observed. Suppression of cell-mediated immunity, as measured by lymphocyte transformation responses, could be demonstrated at the time of recrudescence. Treatment with adrenocorticotropic hormone and trigeminal neurotomy also resulted in recrudescence of IBRV, but immunosuppression was not detected in these animals. The detection of specific, antigen-induced, lymphocyte transformation responses at the time of recovery from both primary and recurrent infections suggests that cell-mediated immunity may be important in determining the duration and severity of the recurrent infection. However, immunosuppression may not be the direct mechanism of recrudescence. Lesions were not observed in untreated animals, and virus could not be detected in either tissues or secretions. However, both lesions and virus were found consistently after corticosteroid treatment.


Immunization of mice with live attenuated encephalomyocarditis virus: local immunity and survival. Mice were vaccinated with an attenuated encephalomyocarditis (EMC) virus strain by the intraperitoneal (i.p.) route and by various ways of respiratory administration: aerosol exposure and intratracheal (i.t.) and intranasal (i.n.) instillation. A linear relationship was found between vaccine dose and the resulting serum antibody titer. The effectiveness of the vaccine was determined by measuring the 50% protective doses (ED(50) values) after a lethal challenge with live virulent virus given by the i.p. route. For all three methods of respiratory immunization essentially the same ED(50) value was found, about 200 plaqueforming units (PFU), but i.p. immunization was less effective, the ED(50) value being about 600 PFU. To investigate the protective effect of local immunity, mice were vaccinated i.p. or i.n. and challenged by the i.n. route. The same ED(50) values were found as after i.p. challenge, indicating that the degree of protection afforded by the vaccine depends only on the route of vaccination and not on the route of challenge. This means that protection depends largely on systemic immunity and that local immunity plays only a minor role in this system. The results are discussed in relation to the feasibility of respiratory immunization against animal viruses.


Immunity against parainfluenza-3 virus in cattle: anti-neuraminidase activity in serum and nasal secretion. The antigenicity of two parainfluenza=3 virus strains, a "neuraminidasestrong" and a "neuraminidase-weak," was compared. For both strains the amount of hemagglutinin units was equal. The antibody responses to neuraminidase and hemagglutinin were measured on samples of serum and nasal secretion and were found to be similar, irrespective of the strain used for immunization. Anti-neuraminidase activity was demonstrated in the gel phase of nasal secretion of immunized cattle. Immunglobulin A was found attached to the peplomers of inhibited virus by immuno-electron microscopy.


The presence of a hormone-sensitive cyclase system in the rat aorta and its relation to lipolysis.


Persistent Newcastle disease virus infection in embryonic chicken tracheal organ cultures. The persistent infection of embryonic chicken tracheal organ cultures with Newcastle disease virus (NDV) is described. Tracheal explants remained morphologically intact and were able to support the replication of NDV for 6 months. Peak titers of released virus occurred at 1 week postinfection, whereas maximal immunofluorescence was not observed until 30 days postinfection. The inoculum titer was not critical, and viral persistence resulted with either of two strains of NDV tested. Serum was not required in the medium for explant viability or to maintain the persistent infection. The presence of a contaminating virus morphologically resembling a leukovirus neither altered the course of infection nor affected the survivability of explants. Although interferon was not detected in the culture medium, persistently infected explants were resistant to heterologous viral challenge, and a similar resistant state could be induced in uninfected explants with exogenous interferon or ultraviolet light-inactivated NDV. No evidence was found to implicate antibody as a regulatory factor in the establishment or maintenance of persistence. The results from electron microscopy and immunofluorescence suggest the cells of the subepithelial connective tissue as the site of NDV persistence.


Intracellular measurements in a closed hyperbaric chamber.


Protection of rabbits against local vaccinia virus infection by Brucella abortus and polyacrylic acid in the absence of systemic interferon production. Brucella abortus and polyacrylic acid, two agents whose interferon-inducing capacity in the mouse has been well established, failed to stimulate circulating interferon production in rabbits, yet protected rabbits against local vaccinia virus infection.


A laboratory program for bioinorganic chemistry.


Alveolar cell carcinoma.

Alveolar cell carcinoma.


Drug-albumin interactions using spin labeling.


Acid mucosubstances underlying lipid deposits in ageing tendons and atherosclerotic arteries.


Effect of stimulation and acetylcholine on 32P and 14C incorporation into phospholipids of eel electroplax.


The mitochondrial derivative of the spermatozoon of Drosophila before and after fertilization.


Correlations in differentiation of protophloem sieve elements of Allium cepa root.


Sphingolipid fatty acid composition of rat brain "membranous sacs".


Effect of biliary obstruction on canine plasma and biliary lipids.


Letter: Electron microscope autoradiography of kidney after administration of 210Pb in mice.


Structural analysis of the highly hydrogen bonded B3 fragment of 28S rRNA of Novikoff hepatoma ascites cells.


Quantitative distribution of lipids in mouse total liver, mitochondria and microsomes.


Electrophysiological and behavioral effects of electrical stimulation  of the raphe nuclei in cats.


The electroencephalogram of sheep and goats with special reference to rumination.


Ultrastructure of pig tubal ova. The unfertilized and pronuclear stage.


The haemocytes of Calliphora erythrocephala (Meig.) (Diptera).


Evidence for the existence of serotonin-, dopamine-, and noradrenaline-containing neurons in the gut of Lampetra fluviatilis.


The rostral pars distalis of the pituitary gland of the freshwater and marine alewife (Alosa pseudoharengus). A light and electron microscope study.


The neurohypophysis of the crested newt. 3. Fine structure of the median eminence.


Age changes in the centrally and peripherally located sensory neurons in rat.


Encephalomyocarditis (EMC) virus infection of the mouse aorta. An ultrastructural study.


Epidemiology of respiratory syncytial virus infection in Washington, D.C. II. Infection and disease with respect to age, immunologic status, race and sex.


Rheumatoid pneumoconiosis. Documentation of onset and pathogenic considerations.


The relationship of erythromonocytic leukemia to other myeloproliferative disorders.


Renin-angiotensin system during labor: evaluation of renin activity in normal, pre-eclamptic, and pre-existing hypertensive patients.


The role of cortisol in human parturition.


Effects of hyperosmolality on transmural sodium transport in the toad bladder.


Ion transport in rabbit ileal mucosa. 3. Effects of catecholamines.


Ion transport in rabbit ileal mucosa. IV. Bicarbonate secretion.


Effects of caffeine on neuromuscular transmission in the rat.


Ornithine decarboxylase stimulation by 3',5'-cyclic AMP in oviduct of Coturnix quail.


Mucosal cyclic AMP and secretion in the dog stomach.


Phosphorylase, calcium, and cyclic AMP in smooth-muscle contraction.


The effects of protein synthesis inhibition on sleep in mice.


The relationship of REM sleep with learning and memory in mice.


Skin window macrophages in malignant lymphomas.


Glucose-mediated insulin release: 3',5' cAMP phosphodiesterase.


Insulin and insulinlike activity in extracts of tumors associated with hypoglycemia.


Propanidid (Epontol)--a new activation method in electroencephalography.


Studies of the habenulo-interpeduncular pathway in cats.


Enzymes of cholesterol ester metabolism in the brain of mutant mice, quaking and jimpy.


Concerning recurrent collaterals and afferent fibers in the hypoglossal nerve of the rat.


Stature and aging.

Stature and aging.


The equilibrium constants of the adenosine triphosphate hydrolysis and the adenosine triphosphate-citrate lyase reactions.


Carcinoma in pleomorphic adenomas of salivary glands.


Characteristics of a soluble gonadotropin receptor from the rat testis.


The 3'-terminal heptanucleotide sequence of the l strand of T7 deoxyribonucleic acid.


Adenosine cyclic 3':5'-monophosphate-mediated transport of neutral and dibasic amino acids in jejunal mucosa.


Partial purification and characterization of nicotinamide adenine dinucleotide kinase from sea urchin eggs.


Isolation of a disulfide-stabilized, three-chain polypeptide fragment unique to the precursor of human collagen.


Selective acylation of 1-acylglycerophosphorylinositol by rat brain microsomes. Comparison with 1-acylglycerophosphorylcholine.


Oligomycin resistance of mitochondrial adenosine triphosphatase in a pleiotropic chromosomal mutant of a "petite-negative" yeast, Schizosaccharomyces pombe.


Regulation of glutamate metabolism and interactions with the citric acid cycle in rat heart mitochondria.


An effect of insulin on cyclic adenosine 3':5'-monophosphate phosphodiesterase activity in fat cells.


Substrate-induced difference spectral, electron paramagnetic resonance, and enzymatic properties of cholesterol-depleted mitochondrial cytochrome P-450 of bovine adrenal cortex.


Oestrogen receptors in the mammary gland of the lactating rat.


Steroid metabolism by adult rat adrenocortical cells in monolayer culture.


Mechanism of oestradiol action on the rat uterus: independence of cyclic AMP, prostaglandin E2 and beta-adrenergic mediation.


Intrinsic stimulatory action of follicle-stimulating hormone on ovarian adenylate cyclase.


Evaluation of the efficacy of ascorbic acid in prophylaxis of induced rhinovirus 44 infection in man.


Bactericidal antibody in serum during infection with Bordetalla pertussis.


Absence of relationship of fecal Clostridium perfringens to rheumatoid arthritis and rheumatoid variants.


Glutamate-mediated respiration in tumors.


Pathology of intestinal neoplasms and other lesions in rats exposed to azoxymethane.


Brief communication: carcinoembryonic antigen in colon cancer: absence in perchloric acid precipitates of plasma.


Brief communication: antigenic relationship between Mycobacterium bovis (BCG) and a guinea pig hepatoma.


Freeze-etching, scanning, and thin-section electron microscopic studies of the "hairy" leukocytes in leukemic reticuloendotheliosis.


Clinical and laboratory investigations on man: systemic administration of potent interferon to man.


Difference in heat stability of antigens associated with Epstein-Barr virus, demonstrated by immunodiffusion.


Expression of malignancy in interspecies Chinese hamster X mouse cell hybrids.


Pathogenesis of feline viral fibrosarcomas: dose and age effects.


A possibility of distinction between normal and neoplastic cells through transplantation into chick blastoderms.


Protective effect of the double-stranded polyribonucleotide, polyinosinic polycytidylic acid, against rat erythroblastosis induced by murine erythroblastosis virus.


Cell-associated virions and nucleoids of a murine leukemia virus.


Tumorigenicity and antigenicity of mouse cells infected with Simian virus 40. I. Relationship of growth in vitro and in vivo in immunosuppressed and immunocompetent recipients.


Oncogenic Marek's disease herpesvirus in avian encephalitis (temporary paralysis).


Nonimmune rosette formation by lymphoma and leukemia cells from Herpesvirus saimiri-infected owl monkeys.


Tumors in CF-1 mice exposed for six consecutive generations to DDT.


Tumors of the kidneys, synovia, exocrine pancreas and nasal cavity in BALB-cf-Cd mice.


Granular cell myoblastoma occurring in the lower lip.


Alphafetoprotein in testicular tumors.

Alphafetoprotein in testicular tumors.


How mercurial diuretics work.

How mercurial diuretics work.


The mineral content of inguinal nodes in barefoot people with and without elephantiasis of the legs.


Parathyroid hormone secretion in familial vitamin-D-resistant rickets.


Thermal synthesis of amino acids from a simulated primitive atmosphere.


Specific neutralization of human hepatitis type A in marmoset monkeys.


Transformation of mammalian cells in vitro by low doses of X-rays.


Acetylcholine release within the cat striatum during the sleep-wakefulness cycle.


Complementation analysis of maple syrup urine disease in heterokaryons derived from cultured human fibroblasts.


Role of inositol cyclic phosphate in stimulated tissues.


Letter: Accelerated substrate cycling of fructose-6-phosphate in the muscle of malignant hyperthermic pigs.


Renin in anesthetized rats.

Renin in anesthetized rats.


Morphologically "differentiated" mouse neuroblastoma cells induced by noncyclic AMP agents: levels of cyclic AMP, nucleic acid and protein.


Analysis of tremorgenic effects of intracaudate serotonin.


Maturity of the pituitary-adrenal axis in the newborn dog.


The selective inhibition of secondary IgE antibody response in mice by dibutyryl adenosine 3',5'-cyclic monophosphoric acid and theophylline.


Effects of urethane on pituitary-adrenal function in the rat.


Induction of immunity in man by crystalline adenovirus type 5 capsid antigens.


Maternal-fetal endocrine interrelations in the rat: effects of cyclic and dibutyryl cyclic AMP on pituitary-thyroid and adrenocortical systems in mother and neonate.


Correspondence: On the ESR spectra of radicals produced by the reaction of OH with uracils.


Sites of x-irradiation-induced damage in the microsomal drug metabolizing enzyme system of rat liver during development.


A semiempirical study of the sugar radicals in irradiated cytidylic acid b and deoxyadenosine.


Invasive mole and gestational choriocarcinoma in Denmark, 1940-1969. Biological and clinical aspects.


Primary hepatic tumors in infancy and childhood.


Entrapment of ophthalmic ointment in the cornea.


Successfully treated postoperative Candida parakrusei endophthalmitis.


Juvenile angiofibroma. Review of 120 cases.


Malignant tumors of the submaxillary gland.


Current surgical management of functioning islet cell tumors of the pancreas.


Airborne stability of simian virus 40.

Airborne stability of simian virus 40. The influence of relative humidity on the airborne survival of simian virus 40 (SV40) was studied by allowing virus aerosols to age in rotating drums at 21 or 32 C and at a relative humidity (RH) value ranging from 22 to 88%. Airborne SV40 virus was stable at every RH tested at 21 C, but aerosols maintained at 32 C were inactivated within 60 min at mid-range RH values. The unusual stability at 21 C over a broad RH range indicates that potentially biohazardous situations may occur under laboratory conditions if this virus becomes accidentally airborne.


Filtration methods for recovering enteroviruses from foods. A set of simple, rapid filtration methods has been developed for recovering enteroviruses from foods. The methods employ quite inexpensive equipment, and the resulting extract might be concentrated in any of several ways. Three model enteroviruses were apparently recovered with at least 80% efficiency when inoculated, at levels ranging from thousands to one plaque-forming unit per 20-g sample, into eleven foods representing six major food groups.


Influence of parabiosis on tumour growth in tumour bearing animals.


Mass airflow cabinet for control of airborne infection of laboratory rodents. A mass airflow cabinet for handling and housing of laboratory rodents has been developed and tested. The unit consists of a high-efficiency particulate air filter and uniform distribution of air at a vertical velocity of 19 cm per s. Animals are maintained without bedding in mesh-bottomed cages that rest on rollers for rotation inside the cabinet. There is an air barrier of 90 cm per s separating the cabinet air from room air. Sampling for airborne bacteria yielded an average of 0.03 colony-forming units (CFU) per ft(3) of air inside the cabinet, whereas 28.8 CFU per ft(3) was simultaneously detected outside the cabinet during housekeeping, a reduction of almost three logs. The efficiency of the air barrier was tested by aerosolization of T3 phage. When phage was aerosolized 5 cm outside the cabinet, no phage could be detected 5 cm inside when the fans were operating; with the fans off an average of 1.6 x 10(4) plaque-forming units (PFU) per ft(3) was detected in six tests. Aerosolization of phage inside the cabinet yielded an average of 9 x 10 PFU per ft(3) outside; an average of 4.1 x 10(6) PFU per ft(3) were detected with the fans not in operation, a reduction of more than four logs. In-use studies on effectiveness showed that the cabinet significantly reduced the incidence of mice originally titer-free to Reo-3 virus. Hemagglutination inhibition antibodies to Reo-3 were detected in 9/22 (42%) mice housed in a conventionally ventilated animal laboratory while no seroconversion was detected in any of 22 mice housed in the mass air flow cabinet in the same laboratory.


Herpes zoster myelitis. Evidence for viral invasion of spinal cord.


Asbestos-induced intrathoracic tissue reactions.


Cytomegalovirus vasculitis with fatal colonic hemorrhage.


An ESR study of the active-site conformations of free and immobilized trypsin.


EPR study of nitric oxide-treated reduced ceruloplasmin.


Arabinose (fucose) dehydrogenase from pig liver. I. Isolation and characterization.


Multiple forms of adenosine 3',5'-cyclic monophosphate-dependent protein kinase from monkey skin.


Cellular reaction in trophoblastic tumours. The presence of a mononuclear cell reaction to 41 gestational choriocarcinomata, 10 invasive moles and 13 malignant trophoblastic teratomata has been investigated. The intensity of the reaction was graded; there was a significantly better response to therapy and survival rate in those with a "severe" cellular reaction than in those with a "mild" reaction to gestational choriocarcinoma. The pathological and clinical features of invasive moles showed no relationship with the cellular reaction to the tumour. The cellular reaction to trophoblastic teratomata was generally poor but there was a marked cellular reaction to the tumour of one patient who has enjoyed a sustained remission.The relationship of cellular reaction and response to treatment with other histological and clinical features was examined. With the exception of a positive correlation between the degree of vascular invasion and response to treatment, none was found.It is suggested that an infiltrate of mononuclear cells in gestational choriocarcinoma is probably a response to the presence of tumour antigens. The infiltrate favourably affects the response to chemotherapeutic agents, suggesting that it contributes to tumour cell death and it may be interpreted as an immunological response directed at tumour rejection.


The effect of nociceptive cutaneous stimuli on human motoneurons.


Some EPR signals in tumour tissue.

Some EPR signals in tumour tissue. Normal and tumour tissues from rats, blood from normal and tumour bearing rats, and normal human blood were examined using the electron paramagnetic resonance (epr) technique. At low temperature a triplet epr signal, which is known to be produced by a NO-haemoprotein complex, was detected in some tumour samples and in decaying normal liver. At room temperature all of the tumour samples examined gave a doublet signal. This signal was also detected in blood but not in other normal tissues. The signal has a g value of 2·0054 ± 0·0002 and a hyperfine splitting of 1·80 ± 0·05 G and is assigned to the ascorbyl free radical. Model experiments suggest that the appearance of detectable concentrations of this radical result from a disturbance of the normal state of the ascorbic acid, dehydroascorbic acid redox system. It was verified that cell division is not responsible for the ascorbyl radical although autolysis may be involved. A possible relationship between the formation of ascorbyl radicals and other paramagnetic species in tumours is discussed.


Immunologic status in lung cancer.

Immunologic status in lung cancer.


Radiotherapy for suspected lung cancer not proved by tissue diagnosis.


Type 4 hyperlipoproteinemia: one theory of atherogenesis.


Neuro-endocrine correlates of immune response. I. Effects of brain lesions on antibody production, Arthus reactivity and delayed hypersensitivity in the rat.


Studies with two new phosphodiesterase inhibitors (ICI 58, 301 and ICI 63, 197) on anaphylaxis in guinea pigs, mice and rats.


Differentiation of T cells induced by preparations from thymus and by nonthymic agents.


Lymphocyte activation in subacute sclerosing panencephalitis virus and cytomegalovirus infections. In vitro stimulation in response to viral-infected cell lines. Efforts to stimulate lymphocytes from measles seropositive and two patients with subacute sclerosing panencephalitis (SSPE) with either commercially available measles virus or virus isolated from a known case of SSPE failed to show any significant data using a microculture assay. Similar results were obtained using lymphocytes from two patients with active cytomegalovirus (CMV) infections and CMV seropositive individuals using CMV suspensions. On the other hand, lymphocytes from the patients with subacute sclerosing panencephalitis exhibited in vitro blastogenesis in culture with SSPE virus-infected HeLa cells. Similarly, lymphocytes from the CMV-infected patients demonstrated blastogenesis when cocultivated with CMV-infected WI-38 cells. This affords a new method for determining the cell-mediated immune capacity of patients with "slow" virus diseases.


Studies on membrane-bound receptors for antigen. Preparation of populations of receptor-depleted lymphocytes. THE EFFECT OF POLYADENYLIC: polyundylic acid complexes (poly A:U) on the amount of antibody on the surface of various populations of mouse lymphoid cells has been investigated by means of a sensitive measure of such activity-the binding by primed cell populations of beta-galactosidase (betaGZ) as an antigen. The sensitivity derives from the liberation of fluorescein from an artificial substrate, fluorescein-di-beta-galactopyranoside (FDbetaG). After incubation with 100 ng/ml of poly A:U, only 40% of the cells previously showing antigen-binding were still active. The optimum range of activity lay between 0.01-1.0 microg/ml poly A:U. Such cells showed increased RNA and protein synthesis as indicated by [(3)H]uridine and [(14)C]amino acid incorporation. The polynucleotide effect was abolished by incubation of the cells with sodium azide or iodoacetate, but not by puromycin. When the proteins on the cell surface were labeled by (125)I, poly A:U caused their release into the medium. Reports by others that the enhancing effect of polynucleotides on the immune response involves the adenylcyclase system are consistent with the finding reported here that reduction of binding by dibutryl 5'-cyclic monophosphoric acid (cAMP) and poly A:U were parallel in extent, and that theophylline and poly A:U acted synergistically in suboptimal concentrations of each.


Varicella-zoster virus.

Varicella-zoster virus.


Experimental human reinfection with herpes simplex virus.


The significance of surface changes in parakeratotic horn.


Early response of lymph node cells to Abelson leukemia virus.


Hexachlorophene neuropathy in rats.

Hexachlorophene neuropathy in rats.


Induced protein synthesis and oestradiol binding to the nuclei in the rat uterus.


Cytology and cytochemistry of the scolex gland cells in Diphyllobothrium ditremum (Creplin, 1825).


Ultrastructure of the gonadal wall of the sea cucumber, Leptosynapta clarki (Echinodermata: Holothuroidea).


The granule-containing cell somata in the superior cervical ganglion of the rat, as studied by a serial sampling method for electron microscopy.


Electron microscopic identification of several types of endocrine cells in the bronchial epithelium of human foetuses.


On structural patterns of the lamina propria of human seminiferous tubules.


The development of a comprehensive treatment programme. Experiences from the after-care service of a mental hospital.


Effects of repeated simulated sonic booms of 1.0 PSF on the sleep behavior of young and old subjects.


Quantitation of three subpopulations of human serum very low density lipoproteins.


Practical considerations in angiotensin II antibody production.


Functional and structural changes in liver mitochondria of rats due to CC14 intoxication. I. Studies on state of electron-transport chain.


Functional and structural changes in liver mitochondria of rats due to CCl4 intoxication. II. Respiratory chain and ion transport.


Trophic influences of the nervous system: their biochemical basis and disturbance in skeletal muscle.


Vestibular ototoxicity in primates.

Vestibular ototoxicity in primates.


Some biochemical and pharmacological actions of (-) erythro-meta-(meta-chlorobenzyloxy)-2-(1-aminoethyl)-benzyl alcohol. A derivative of metaraminol.


Bradykinin potentiating peptide PCA-Lys-Trp-Ala-Pro. An inhibitor of the pulmonary inactivation of bradykinin and conversion of angiotensin I to II.


Effect of 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane on adrenocorticotropic hormone-induced steroidogenesis in various preparations in vitro of dog adrenal cortex.


Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung.


Inner ear concentrations and ototoxicity of different antibiotics in local and systemic application.


Effect of 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane in vivo on baseline and adrenocorticotropic hormone-induced steroid production in dog adrenal slices.


Chemical and structural relationships of NAD+ and platinum binding to malate dehydrogenase.


Determination of the zero-field splitting in human acid methemoglobin by millimeter and submillimeter ESR experiments.


Ferredoxin from Veillonella alcalescens.


Evaluation of dipolar nuclear magnetic resonance shifts in low-spin hemin systems: ferricytochrome c and metmyoglobin cyanide.


Chemical modification of Ehrlich ascites tumour cells by periodate and succinic anhydride: effects on metabolism and membrane permeability.


Amino acid dependent ATP-32PPi exchange measurement. A filter paper disk method.


Light induced H+ uptake catalysed by photochemical reaction centres from Rhodopseudomonas spheroides R26.


Separation of the isoenzymes of lactate dehydrogenase by affinity chromatography using an immobilized AMP-analogue.


Synthesis of antibiotics by enzymes from altered growth conditions by Bacillus licheniformis.


Spin-spin interaction between iminoxyl radicals localised in antibody combining sites.


Purification of estradiol receptor by affinity chromatography. Representative experiments.


Extrachromosomal element delta in Drosophila melanogaster. IX. Induction of delta-retaining chromosome lines by mutation and gene mapping. [Delta b], symbolized as [delta(b)], is retained by S(b) chromosome lines and transmitted through the females to their progeny. Transmission through the males is not directly demonstrable (Minamori 1969a). [delta r], symbolized as [delta(r)], is retained by S(r) chromosome lines and transmitted biparentally (Minamori 1971). The multiplication of delta is suppressed at low temperature. All descendant lines derived from S(b)-carrying or S(r)-carrying flies in which the presence of delta cannot be demonstrated gradually accumulate their specific delta factors over many generations (Minamori 1969b, 1972). The delta factors and the sensitive chromosomes are inseparably associated. This observation led to the assumption that delta may be a copy of a chromosomal gene or a certain agent integrated into the chromosome (Minamori 1972). This assumption was examined in the present study by experiments designed to induce delta-retaining sensitive chromosomes, and to map the gene(s) responsible for delta-retention and/or for sensitivity to the killing action of delta factor. One sensitive chromosome which retained [delta(b)] (S(b) chromosome) was obtained in the presence of [delta(b)] out of 2492 insensitive chromosomes which retained no delta; in addition one S(b) chromosome was obtained in the presence of [delta(r)] out of 2131 insensitives. The latter finding suggests that S(b) might be induced by a mutation caused by [delta(b)] or [delta(r)], but not by integration of either delta into the chromosome. Four S(b) chromosomes and one sensitive chromosome which retained [delta(r)] (S(r) chromosome) were obtained out of 1970 insensitives when males carrying the chromosome were fed an alkylating mutagen, ethyl methane sulfonate (EMS). The location of delta-retaining genes was examined by crossing-over experiments employing eight S(b) and five S(r) chromosomes. The genes on these chromosomes were found to be located in the same region or near one another. The gene for [delta(b)], symbolized as Da(b), and the gene for [delta(r)], symbolized as Da(r), are assumed to be multiple alleles of a locus at 2-24.9. The sensitivity of the chromosomes was modified appreciably by recombination; hence, the genes controlling this trait are assumed to be a polygenic system. The findings obtained in this study lead to the hypothesis that delta may be produced by a chromosomal gene (Da) and transmitted extrachromosomally.


Temperature-sensitive mutations in Drosophila melanogaster. XVII. Heat- and cold-sensitive lethals on chromosome 3. Ethyl methanesulfonate-treated third chromosome of Drosophila melanogaster were tested for the presence of dominant and recessive temperature-sensitive lethal mutations at 17 degrees , 22 degrees and 29 degrees C. Out of 1,176 chromosomes tested, no dominant ts lethals, 21 heat-sensitive, 22 cold-sensitive and 10 heat-cold-sensitive lethals were recovered. Heat-cold sensitivity was produced by a single mutation in all cases. Sixty-two percent of the ts lethals were fertile as homozygotes in both sexes. Surprisingly, 88% of the ts lethals mapped between st and Sb, a region straddling the centromere and estimated to comprise 12.9% of the genetic length and 55% of the physical length of chromosome 3. All but one of the heat- and cold-sensitive lethals complemented with each other at their respective restrictive temperatures.


Xeroradiography of the breast.

Xeroradiography of the breast.


Observations on the intranasal vaccination of pregnant cattle against infectious bovine rhinotracheitis and parainfluenza-3 virus infection.


Separation and identification of food dyes. V. Examination of ponceau 6R dyes: extraction of dyes from confectionery products (cakes, cake mixtures and pastries).


The interaction between Mycoplasma hominis and poliovirus in cell culture.


The tract of Lissauer in relation to sensory transmission in the dorsal horn of spinal cord in the macaque monkey.


Comparison of the aerosol stabilities of foot-and-mouth disease virus suspended in cell culture fluid or natural fluids.


Natural selection of mutants of vesicular stomatitis virus by cultured cells of Drosophila melanogaster.


Isolation and characterization of virus-resistant mouse embryo fibroblasts.


The fragmentation of cellular DNA and the formation of pseudovirions during SV 40 infection of African green monkey kidney cells.


Concentration of Epstein-Barr virus from cell culture fluids with polyethylene glycol.


Evaluation of the methods of differentiation of variola and alastrim viruses.


Results of a virological study of smallpox convalescents and contacts: short communication.


Serological types of whooping cough bacteria and their connection with immunity in vaccinated children.


Large-scale production of clostridial collagenase and lecithinase C: short communication.


Distribution and relationship of precursor Z material to organizing myofibrillar bundles in embryonic rat and hamster ventricular myocytes.


Glycolytic and tricarboxylic acid cycle intermediates during cardiac arrest and recovery in eu-, hyper- and hypothyroid rats.


Abrupt reoxygenation of the anoxic potassium-arrested perfused rat heart: a study of myocardial enzyme release.


The S-100 protein in water-soluble and pentanol-extractable form in normal human brain and tumours of the human nervous system. A quantitative study.


Sporadic acrodystrophic neuropathy.

Sporadic acrodystrophic neuropathy.


Hallervorden-Spatz disease and infantile neuroaxonal dystrophy. Ultrastructural observations, anatomical pathology and nosology.


Interaction of mouse strain and differential housing upon resistance to Plasmodium berghei.


The effect of beta-lipoprotein on antistreptolysin-O antibody titers.


A radioimmunoassay of the antigen-binding capacity of rabbit antiserum prepared with axenically grown Entamoeba histolytica, Laredo strain.


International symposium on Hodgkin's disease. Session 2. Histopathology and experimental cytology. Invited discussion: Synthesis and implications for the etiology, pathogenesis and spread of Hodgkin's disease.


The activity of some nitroso compounds in the mouse dominant-lethal mutation assay. I. Activity of N-nitroso-N-methylurea, N-methyl-N-nitroso-N'-nitroguanidine and N-nitrosomorpholine.


International Symposium on Hodgkin's Disease. Session 1. Epidemiology and etiology. Part 2. Virology and serology--new approaches to identification of an etiologic agent.


Oncogenic herpesviruses: a selective review and their possible inplications for Hodgkin's disease.


Is Epstein-Barr virus a human tumor virus?


Invited discussion: relationship of titers of Epstein-Barr virus to cell-mediated immunity in patients with Hodgkin's disease.


Epstein-Barr virus-related serology in Hodgkin's disease.


Burkitt's lymphoma and heredity.

Burkitt's lymphoma and heredity.


Evaluation of the hand and its function.


Rehabilitation of the hand with motor and sensory impairment.


The place of microsurgery in hand surgery.


Interaction of isonicotinoyl hydrazide-adenine-dinucleotide with dehydrogenases.


Freeze-etching electron microscopy of the lung.


Endometrial cancer. Facts and theories.

Endometrial cancer. Facts and theories.


Pediatric familial type II hyperlipoproteinemia: therapy with diet and cholestyramine resin.


California arbovirus (La Crosse) infections. I. Clinical and laboratory findings in 66 children with meningoencephalitis.


Stimulation of steroid secretion in adrenal tumor cells by choleragen. Choleragen, the pure protein from cholera toxin, stimulates steroid secretion by Y-1 adrenal tumor cells in culture. The secreted steroids are the same as seen after addition of adrenocorticotropic hormone. Half-maximal stimulation occurs at 15 pM; stimulation is essentially irreversible by washing and partially reversible (for about 1 hr) by antibody, and there is a latent period of about 60 min before stimulation is seen. Stimulation of adenylate cyclase occurs at about 30-fold higher choleragen concentrations. Gangliosides inhibit choleragen stimulation when added before but not after the toxin. Lipopolysaccharides from Escherichia coli, Salmonella typhosa, and Serratia marcescens also stimulate steroid secretion, but are less potent than choleragen.


Specific recognition of plasma membranes by embryonic cells. Methods have been developed for preparation of plasma membrane fractions from embryonic neural retina and cerebellum. These membrane fractions are specifically bound by intact cells of the original tissue (homotypic binding) and not by cells from the other tissue (heterotypic binding). Aggregation of neural retina cells and cerebellar cells is prevented by addition of homotypic membranes but not by heterotypic membranes. We conclude that, under our assay conditions, these embryonic cells specifically recognize homotypic membranes, and that the specificity of recognition is the same as in the initial step in the process of cellular aggregation.


Familial hypercholesterolemia: identification of a defect in the regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity associated with overproduction of cholesterol. The homozygous form of the autosomal dominant disorder, familial hypercholesterolemia, is characterized by the presence in children of profound hypercholesterolemia, cutaneous planar xanthomas, and rapidly progressive coronary vascular disease that usually results in death before age 30 years. Cultured skin fibroblasts from three unrelated subjects with this disorder showed 40- to 60-fold higher activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.34), the rate-controlling enzyme in cholesterol biosynthesis, when compared with fibroblasts of seven control subjects. Enhanced enzyme activity resulted from a complete absence of normal feedback suppression by low-density lipoproteins, which led to a marked overproduction of cholesterol by the mutant cells. The demonstration of apparently identical kinetic properties of the reductase activity of control and mutant cells, coupled with the evidence that this enzyme is normally regulated not by allosteric effectors but by alterations in enzyme synthesis and degradation, suggests that the primary genetic abnormality does not involve the structural gene for the enzyme itself, but a hitherto unidentified gene whose product is necessary for mediation of feedback control by lipoproteins. The fibroblasts of two obligate heterozygotes, the parents of one of the homozygotes, showed a pattern of enzyme regulation intermediate between that of controls and homozygotes.


Prediction of the amount of secondary structure in a globular protein from its aminoacid composition. Multiple regression is used to obtain relationships for predicting the amount of secondary structure in a protein molecule from a knowledge of its aminoacid composition. We tested these relations using 18 proteins of known structure, but omitting the protein to be predicted. Independent predictions were made for the two subchains of hemoglobin and insulin. The average errors for these 20 chains or subchains are: helix +/- 7.1%, beta-sheet +/- 6.9%, turn +/- 4.2%, and coil +/- 5.7%. A second set of relations yielding somewhat inferior predictions is given for the case in which Asp and Asn, and Glu and Gln, are not differentiated. Predictions are also listed for 15 proteins for which the aminoacid sequence or tertiary structure is unknown.


Methionine transfer ribonucleic acids of avian myeloblastosis virus. Chick-embryo cells contain four isoaccepting species of methionine transfer RNA (I-IV). One species (I) is the initiator, tRNA(f) (Met), and the others (II, III, and IV) are the donors of internal methionyl residues (tRNA(m) (Met)). Over 85% of the tRNA(Met) in purified avian myeloblastosis virus consists of one tRNA(m) (Met) species, which resembles host-cell tRNA(Met) IV with respect to chromatographic properties on RPC-5, electrophoretic mobility of the terminal methionyl-oligonucleotide, and activity in cell-free protein synthesis in response to synthetic and natural messenger RNAs. Thus, tRNA(Met) IV of avian myeloblastosis virus is structurally indistinguishable from the corresponding host-cell species, and, although not involved in initiation of viral protein synthesis, is able to function in peptide-chain elongation.


ATPase and GTPase activities associated with a specific 5S RNA-protein complex. An RNA-protein complex consisting of 5S RNA and two ribosomal proteins, B-L5 and B-L22, was isolated from Bacillus stearothermophilus ribosomes and found to be active in GTP hydrolysis. This activity was not influenced by elongation factor G. Further analysis of this complex showed that it was also able to hydrolyze ATP. Inhibition studies revealed that ATP was a noncompetitive inhibitor of GTP and that GTP was also a noncompetitive inhibitor of ATP, indicating that two different enzymatic sites were involved. Differences in pH optimum and optimal temperature also point to a twosite enzyme complex. Both enzymatic hydrolyses were inhibited by thiostrepton and fusidic acid, which are known inhibitors of protein synthesis.


Isolation of circular DNA molecules from whole cellular DNA by use of ATP-dependent deoxyribonuclease. A technique is described for isolation of plasmid DNA in closed and open circular double-stranded forms from bacterial cells, by use of ATP-dependent deoxyribonuclease purified from Micrococcus luteus. This DNase, acting only upon linear DNA molecules, degrades all bacterial chromosomal DNA extracted in the linear form. Circular plasmid DNAs are left intact, and are then separated by sedimentation through a sucrose gradient. Unlike previous techniques for analysis of plasmid DNA, this technique can be used to isolate not only closed circular DNA but also open circular DNA. Several plasmids, such as those from phage (lambdadv1 and lambdadv21), a colicinogenic factor (Col E2), a sex factor (F(8)' gal), and "minicircles" in Escherichia coli 15, in both the open and closed circular forms, were well separated from chromosomal DNA by this technique.


Linear association between cellular DNA and Epstein-Barr virus DNA in a human lymphoblastoid cell line. High-molecular-weight DNA from cell line Raji (derived from Burkitt's lymphoma), which contains 50-60 copies of Epstein-Barr virus DNA per cell, was fractionated in neutral solution by several cycles of CsCl gradient centrifugation in fixed-angle rotors. Under the fractionation conditions used, intact Epstein-Barr virus DNA from virus particles can be separated from the less-dense cellular DNA. In contrast, a large proportion of the intrinsic Epstein-Barr virus DNA component of Raji cells remains associated with cellular DNA, as determined by nucleic acid hybridization. This interaction, which is resistant to Pronase and phenol treatment, is not the result of aggregation. When the molecular weight of Raji DNA is reduced by hydrodynamic shear, the amount of virus DNA associated with cell DNA decreases. However, some virus DNA still remains bound to fragments of cellular DNA after shearing. The association is completely destroyed in alkaline solution. Molecular weight analysis of Raji DNA after denaturation showed that the alkali-induced release of Epstein-Barr virus DNA was specific and not the result of random single-strand breaks. These data indicate that Epstein-Barr virus DNA is linearly integrated into Raji cell DNA by alkali-labile bonds.