The effect of hypertonic media on water permeability of frog urinary bladder. Inhibition by catecholamines and prostaglandin E 1 . The frog urinary bladder undergoes, in some conditions, a marked increase of its water permeability when incubated in hypertonic media. This increase was observed with various nonpermeant solutes. It seems to result from the shrinkage of an osmo-sensitive compartment of the tissue, probably the epithelial cells. Many similarities were found between this effect and the physiological increase in water permeability (hydrosmotic response) elicited by antidiuretic hormone (ADH): both were dependent on the physiological state of the animals, and although the response was slower after hyperosmolar than after hormonal challenge, the patterns of response were similar, and in both cases markedly dependent on bathing solution temperature. Norepinephrine and prostaglandin E(1), which in this tissue reduce the hydrosmotic action of ADH, presumably by inhibiting the adenyl cylase also reduced the effect of hyperosmolarity. Conversely this effect was potentiated by incubation in the presence of oxytocin, exogenous cyclic AMP, and theophylline, conditions in which the intracellular concentration of cyclic AMP is increased. These data demonstrate that the response to hyperosmolarity is elicited, at least partly, by mechanisms also involved in the physiological hydrosmotic response to ADH.


Antimetabolites of coenzyme Q. 16. New alkylmercaptoquinones having antimalarial curative activity.


Isolation and renin-inhibitory activity of phosphoglyceride from shark kidney.


A working hypothesis explaining the maintenance of the transformed state by SV40 and polyoma.


Instability of a layer of chemotactic cells, attractant and degrading enzyme.


Oral carcinoma in the first three decades of life.


The prostatic cell DNA-steroid association: a hypothesis of nuclear events and cytoplasmic consequences.


Antibodies to EBV related antigens in West Malaysian children.


A case of glomus jugulare tumour.

A case of glomus jugulare tumour.


Cellular and hormonal control in glycogen metabolism--recent advances.


Electron microscopic studies on various types of inclusions found in autopsy cases. I. A new type of nuclear inclusion in the liver of polyradiculoneuritis.


Physical studies of membrane structure.

Physical studies of membrane structure.


Efficacy of psychiatric rehabilitation.

Efficacy of psychiatric rehabilitation.


Sleep and memory.

Sleep and memory. Two experiments demonstrated that memory over an interval with relatively high amounts of rapid eye movement (REM) sleep was inferior to memory over an interval with relatively high amounts of stage 4 sleep. The results suggest that, at least for humans, REM sleep does not facilitate memory consolidation and that stage 4 sleep may be beneficial to memory.


A theory and physical model for chochlear mechanics.


Absorption of "penetrating" anions by hydrophobic parts of macromolecules.


Tablet sensitivity testing: a comparison of different methods.


Some characteristics of the A antigen in Marek's disease virus--infected cell cultures.


A micro assay for mouse and human interferon.


Fine structure of melanin-producing aeromonads.


On the adsorption of Australia antigen to a colloidal silica, and some possible applications.


Drug and sociotherapy in the aftercare of schizophrenic patients. One-year relapse rates.


Investigation of the conformational properties of aspartate aminotransferase molecules by the spin-tag method.


Faliure to demonstrate desmosterol in spinal fluid of brain tumor patients.


Granular cells in tumors and in nontumorous tissue.


The effect of cholera toxin on the phosphorylation of protein in epithelial cells and their brush borders.


Subacute sclerosing panencephalitis. Immunoultrastructural localization of measles-virus antigen.


The "oxygenated complex" of cytochrome c oxidase.


Increases in rat liver cyclic AMP concentrations prior to the initiation of DNA synthesis following partial hepatectomy or hormone infusion.


Kinetics of reconstitutioin of polyphenoloxidase from apoenzyme and copper.


Investigation of photoprocesses in aromatic peptides by the method of electron paramagnetic resonance.


The identification and characterization of a cyclic AMP dependent protein kinase on rabbit reticulocyte ribosomes.


Sialyl transferase activity in normal and RNA- and DNA-virus transformed cells utilizing desialyzed, trypsinized cell plasma membrane external surface glycoproteins as exogenous acceptors.


The fluorescence behaviour of 1-anilino-8-naphthalene sulphonate in phospholipid and natural membranes.


Isolated adrenal cortex cells: (Trp(Dnps) 9 )ACTH 5  -24 , an inhibitor of ACTH 1-24 -induced cyclic AMP and corticosterone production.


Transmural and subcellular localization of monoamine oxidase and catechol-0-methyl transferase in rabbit aorta.


Interaction of chloropromazine with adrenochrome and interference of a possible endogenous psychotogenic agent with some synaptic enzyme activities.


Isolation and identification of a renin inhibitor form ox bile.


Assisting patients with posthospitalization plans.


Coupled layers: a new technique for the two-dimensional thin-layer chromatography of carbohydrates.


A method for the determination of Venzar (3-cyclohexyl-5,6-trimethyluracil) on silica gel layers.


Effect of upright posture and isoproterenol infusion on cyclic adenosine monophosphate excretion in control subjects and patients with labile hypertension.


Lack of responsiveness to L-dopa in Cushing's disease.


Control of hypoglycemia with L-asparaginase in a patient with islet cell cancer.


Peroxidase defect in congenital goiter with complete organification block.


Control of aldosterone secretion in hyperthyroidism.


Thickness and area measurements as parameters of skeletal involution of the humerus, femur, and tibia.


Relationship of radiographic cortical thickness vs. age in thirteen bones of the hand.


Studies on nitroxide spin-labeled poly- -benzyl- ,L-glutamate.


Serotonin and inhibition in Limulus lateral eye. The response to light of one ommatidium is reduced or suppressed by simultaneous illumination of neighboring ommatidia. The mechanism of this lateral inhibition may be chemical synaptic transmission, based on the physiological findings of a number of investigators and on the following evidence. The fine structure of the neuropil of the lateral plexus exhibits numerous clear vesicles (ca. 400 A), dense-core vesicles (ca. 700-1400 A), Golgi regions, and other morphological features of neurochemical synapses. The indolealkylamine, serotonin (5-HT), even in nanomolar concentrations, has a potent inhibitory action. An initial, potent inhibitory dose of 5-HT produces a long lasting densensitization to subsequent doses. The desensitization affects lateral inhibition evoked by light stimulation of neighboring receptors, i.e. crossed-desensitization. Eye tissue extracts contain 5-HT and melatonin (MLT) at a level greater than 1 microg/g wet tissue and perhaps as high as 20-30 microg/g, as determined by two-dimensional thin-layer chromatography (TLC) and o-phthaldialdehyde fluorescence assay techniques. Subcellular fractionation on sucrose gradient indicates a peak in 5-HT and MLT content associated with an intermediate density fraction. 5-HT may be an inhibitory transmitter for lateral inhibition. One pathway for metabolism of 5-HT in the lateral eye may be via N-acetylserotonin and melatonin.


Follow-up care after hospitalization. Health center vs hospital.


Effects of dibutyryl cyclic adenosine phosphate plus theophylline on murine sarcoma virus transformed non-producer cells.


Induction of antinuclear antibodies in (CxB6) F1 mice inoculated with Graffi and Rauscher leukemogenic viruses.


Some aspects of the tubular locus of the natriuretic action of vasoactive drugs.


Study of immune cytotoxicity with Herpesvirus hominis infected cells.


Herpes simplex virus replication in human lymphocyte cultures stimulated with phytomitogens and anti-lymphocyte globulin.


Interferon induction in normal leukemic human lymphocyte cultures by tilorone hydrochloride.


Variations in the interferogenic responses of two strains of mice.


The form of absorption of lipids in the chicken, Gallus domesticus.


Heterogeneity of human plasma high density lipoprotein.


Roles of aortic and carotid chemoreceptors in activating the hypothalamo-hypophyseal-adrenocortical system during hypoxia.


Comparative effects of hypoxia, adrenocorticotropin and methylcholine on adrenocortical secretory rates.


Effect of methylprednisolone in epinephrine-thyroxine induced arteriosclerosis.


Interferon inhibition by narcotic analgesics.


Blocking of interferon production by chromatographically purified L cell interferon.


Effect of a converting enzyme inhibitor (SQ 20,881) on angiotensin-induced drinking.


A simple method for the growth of mouse neuron monolayer cultures.


Adipose and liver tissue enzyme profiles in obese hyperglycemic mice.


Inhibition of protocollagen proline hydroxylase by Dilantin.


Interferon antagonists induced by Newcastle disease virus (NDV).


Differentiation of types 1 and 2 herpes simplex virus by plaque inhibition with sulfated polyanions.


Binding of angiotensin I and angiotensin II to plasma proteins precipitated by ammonium sulfate.


The role of angiotensin II in a pressor response to nicotine after phenoxybenzamine.


Stimulation of angiotensinogen formation by renin and angiotensin.


The production of human leukocyte interferon in a serum-free medium.


Interferon and cell division. VII. Inhibitory effect of highly purified interferon preparations on the multiplication of leukemia L 1210 cells.


Histochemical study of the effect of a deuteron beam on the dental germ.


In vitro evaluation of enzymatic debridement of burn wound eschar.


Aspiration cytology in carcinoma of the pancreas.


A summary of the status of de novo cancer in transplant recipients.


The effect of ACTH on corticosteroid concentration in monkey bile, as measured by competitive protein-binding analysis.


Liver transplantation.

Liver transplantation.


Personal experience with orthotopic liver transplantation.


Cyclic AMP in the regulation of gastric secretion in dogs and humans.


Reinforcement of reflex epinephrine release by angiotensin II.


Amino acid transport in bone: stimulation by cyclic AMP.


Carboxylate modification of neuromuscular transmission in the frog.


Responses of isolated monkey umbilical veins to biogenic amines and polypeptides.


A comparison of influenza and parainfluenza and RNP properties.


Induction of interferon and host resistance in vivo by double-stranded complexes of copolyribonucleotide of isosinic and guanylic with polyribocytidylic acid.


Action of angiotensin and plasmakinins on blood flow through the mammary artery in lactating small ruminants.


(2- 14 C) ethanol as a precursor of glutamine, glutamate, -aminobutyric acid and aspartate in hamster brain in vivo.


Mode of action of 5,5'-diphenylthiohydantoin-sites of action of 5,5'-diphenylthiohydantoin in mitochondria in relationship to thyroxine-stimulated responses.


Significance of the LP-X test in differential diagnosis of jaundice.


Effect of diazoxide on golden hamsters with chronic hypoglycemia due to a transplantable islet-cell tumor.


The influence of blood glucose on ACTH secretion in man.


Effects of metopirone on urinary excretion of adrenaline, noradrenaline and vanylmandelic acid in different physiological conditions. The possible role of adrenal cortex in catecholamine degradation.


Calcitonin secretion in medullary carcinoma of the thyroid.


On the effect of synthetic LH-releasing hormone on the blood levels of LH, FSH, HGH, TSH, ACTH, insulin and blood sugar in man.


The formation and distribution of cyclic 3',5'-adenosine monophosphate in the everted guinea pig seminal vesicle sac.


Virus-induced genitourinary malignancy. Macromolecular identity.


Laboratory production and  14 C-labelling of viomycin.


Regulation of insulin secretion in the ovine foetus in utero. Effects of sodium valerate on secretion and of adrenocorticotrophin-induced premature parturition on the insulin secretory response to glucose.


Lipolysis and adenosine 3',5'-cyclic monophosphate in adipose tissue of the New Zealand obese mouse.


Effect of sleep on memory. 3. Controlling for time-of-day effects.


The hyperphosphatemic effect of disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP TM ): renal handling of phosphorus and the renal response to parathyroid hormone.


The pathology and immunology of Bordetella pertussis infection.


The prevention of experimental Clostridium novyi and Cl. perfringens gas gangrene in high-velocity missile wounds by active immunisation.


Petrographic study of the refractory performance of high-fusing dental alloy investments. I. High-fired, phosphate-bonded investments.


The effect of the medium and source of growth factors on the satellitism test for Haemophilus species.


The epidemiology of tanapox and yaba virus infections.


An electron-microscopic study of intracerebral infection of mice with low-virulence Bordetella pertussis.


Steroid metabolism in the adrenals of fetal sheep in relation to natural and corticotrophin-induced parturition.


Parturition in the goat and sheep: changes in corticosteroids, progesterone, oestrogens and prostaglandin F.


The physiological rôles of progesterone, oestradiol-17  and prostaglandin F 2   in the control of ovine parturition.


Determination of steady-state concentrations of adenine nucleotides in growing C. kluyveri cells by biosynthetic labeling.


Chronic active hepatitis and Graves' disease.


Determination of N-(9-( -D-ribofuranosyl)purin-6-ylcarbamoly)threonine at the picomole level in transfer-RNA.


Advantages of the use of Cerenkov vounting for determination of P 32  in photophosphorylation research.


Method for rapid isolation of free aminoacyl-transfer RNA.


Agarose gel electrophoresis of serum lipoproteins: determination of true mobility, isoelectric point, and molecular size.


Simple and sensitive photometric recycling assay for nicotinamide-adenine dinucleotide.


The effect of cyclic adenosine- and guanosine 3',5'-monophosphate on the normal and drug-increased coronary blood flow.


Phosphodiesterase inhibition by papaverine and structurally related compounds.


Transcription of ribonucleic acid by the ribonucleic acid directed deoxyribonucleic acid polymerase of Rous sarcoma virus and deoxyribonucleic acid polymerase I of Escherichia coli.


Butyl isocyanate, an active-site-specific reagent for yeast alcohol dehydrogenase.


Heterogeneity of human plasma very low density lipoproteins. Separation of species differing in protein components.


Purification and kinetic properties of Neurospora transfer ribonucleic acid nucleotidyltransferase.


The role of phospholipid in succinate cytochrome c reductase.


Phosphate transfer from adenosine triphosphate in a model system.


Tissue reactions to the three root canal medicaments.


The effects of polyvinyl pyridine-N-oxide (P204) on the cytopathogenic action of chrysotile asbestos in vivo and in vitro.


Sendai (parainfluenza 1) infection of mouse eggs.


A quantitative investigation of the effects on the growth of calcium oxalate crystals on potential inhibitors.


Biochemical changes in intestinal mucosa after experimental small bowel by-pass in the rat.


Pressor response to angiotensin I and angiotensin II: the site of conversion of angiotensin I.


Protocollagen proline hydroxylase in the eye, cartilage and skin of chicken embryo.


Immunofluorescence for routine diagnosis of respiratory syncytial virus infection. A comparison of immunofluorescent tests for the diagnosis of respiratory syncytial (RS) virus infections was carried out on 42 hospitalized cases of respiratory infection in childhood. Respiratory syncytial virus was detected in 22 (52%) cases, the most sensitive method of detection being by indirect immunofluorescence of Bristol HeLa tissue cultures inoculated with nasopharyngeal aspirates. The highest detection rate was in bronchiolitis cases (92%). Detection of antibody rises in paired sera, eight days apart, confirmed RS virus infection in 13 of 16 cases, the most sensitive test being detection of a specific rise in IgG antibody by indirect immunofluorescence. A serodiagnosis was made in all 10 non-bronchiolitis cases. Recommendations are made for the application ofimmunofluorescence to routine diagnosis of RS virus infection.


Electron spin resonance study of radicals produced by one-electron reduction of pyridines.


Antitumor activity of polyinosinic-polycytidylic acid in combination with some biologically active compounds.


Differences in the primary structure of nucleolar and ribosomal high molecular weight RNA of the novikoff hepatoma and rat liver, and possible therapeutic implications.


Nuclear magnetic resonance studies of rat serum lipoproteins.


Relations between metabolic increase of plasma free fatty acids and the occurrence of arteriosclerotic thromboarteritis in rabbits. Rabbits maintained for several weeks on a regimen of modest amounts of vitamin D and dietary cholesterol were placed in three groups in accordance with their response to repeated subcutaneous injections of nicotine in mineral oil. The group that had the greatest increase in plasma FFA following nicotine injections gradually developed, over a period of about 12 weeks, severe calcific atheroarteriosclerosis with peripheral thromboarteritis. Those that had a moderate increase in plasma FFA following nicotine injections developed calcific atheroarteriosclerosis but no thromboarteritis. Those that had the least increase in plasma FFA following nicotine injections developed no arterial lesions. Comparable or much greater increases in plasma FFA occurred in rabbits on the vitamin D-cholesterol regimen when adrenalin, ACTH or heparin was injected rather than nicotine. These animals did not develop calcific atheroarteriosclerotic thromboarteritis or any other lesions which could be correlated with the increased levels of plasma FFA. Inasmuch as nicotine, vitamin D or dietary cholesterol in the amounts used were innocuous when used alone, the interactions between the effects of at least these three factors need to be known in individual animals before the pathogenesis of the calcific atheroarteriosclerotic lesions with thrombosis can eventually be understood.


A serologic survey of veterinarians for antibody to feline leukemia virus.


Synopsis of vaccination procedures for dogs.


Plasma renin activity and blood pressure in 89 patients receiving maintenance haemodialysis therapy. Blood pressure, plasma renin activity, plasma sodium concentration, plasma potassium concentration, dietary sodium intake, and duration of dialysis have been measured under standard conditions in 89 patients on maintenance haemodialysis. No significant relation was found between plasma renin activity and blood pressure. Statistically significant correlations were found between plasma renin activity and plasma sodium concentration and between plasma renin activity and dietary sodium intake.Only one patient was found to have uncontrollable hypertension associated with a markedly raised plasma renin activity. Reasons are given for not performing bilateral nephrectomy in this patient. We believe the low incidence of uncontrollable hypertension and hyperreninaemia in our patients to be due to their slow introduction to haemodialysis, thus preventing violent swings in body weight, blood pressure, and renin secretion.Although plasma renin activity did fall with duration of dialysis, all 15 patients who have been on maintenance dialysis for longer than five years have normal levels.


Oxidative phosphorylation in mitochondria during embryonic development.


Changes in plasma insulin during ethanol-induced hypoglycemia.


Thiazide diuretics and calcium metabolism.


The effect of initial body weight on the response of the rat to treatment with gonadotrophins.


The effect of initial age on the response of the rat to treatment with gonadotrophins.


Response of plasma aldosterone to angiotensin II, ACTH and potassium in man.


Familial poly-endocrinopathy.

Familial poly-endocrinopathy.


Respiration of homogenates and mitochondrial fractions of bovine dental pulp.


Genetics of human-mouse somatic cell hybrids: linkage of human genes for isocitrate dehydrogenase and malate dehydrogenase.


Mitochondrial polymorphism. IV. Phospholipid composition of mitochondria of a wheat hybrid and its parents.


Determination of adenosine 3':5'-cyclic monophosphate in cerebral tissues by saturation analysis. Assessment of a method using a binding protein from ox muscle. 1. The Gilman (1970) procedure for determining cyclic AMP (adenosine 3':5'-cyclic monophosphate) by saturation analysis gave erroneous results when applied to the analysis of extracts of whole brain or preparations of membrane fragments from brain. 2. The extracts contained a non-diffusible factor, which enhanced the binding of cyclic AMP by the muscle protein fraction. 3. Extracts also contained material which inhibited binding, but net inhibition of binding was only observed when relatively concentrated extracts were analysed. 4. The error introduced by the factors modifying binding could be eliminated by incorporation of unlabelled internal standards in the unknowns. The design adopted enables a statistical estimate to be made of the standard error of a single assay. 5. The modified assay was used to determine bound cyclic AMP and adenylate cyclase activity in cerebral membrane fragments. Five preparations of synaptic membrane fragments contained less than 3.5pmol of cyclic AMP/mg of protein; a microsomal fraction from rat contained 65pmol of cyclic AMP/mg of protein.


Cell biology of the mycoplasmas.

Cell biology of the mycoplasmas.


The polyamine content in two slow-growing rat hepatomas.


A heat-stable nicotinamide-adenine dinucleotide glycohydrolase from Pseudomonas putida KB1. Partial purification and some properties of the enzyme and an inhibitory protein. A thermostable NAD(P)(+) glycohydrolase (EC 3.2.2.6) detected in cell-free extracts of Pseudomonas putida KB1 was purified to a single component on polyacrylamide-gel electrophoresis. A heat-labile inhibitor of the enzyme was also partially purified. Enzyme free of inhibitor is present in culture supernatants. After an ultrasonic treatment enzyme-inhibitor complex and excess of inhibitor are present in both the cell-debris and soluble fractions. The general properties of the enzyme and inhibitor are described. The molecular weights of enzyme, inhibitor and enzyme-inhibitor complex, determined by gel filtration are about 23500, 15000 and 35000 respectively. The binding of inhibitor and enzyme is inhibited by the presence of substrate.


The structure of a glycerylphosphoryldiglucosyl diglyceride from the lipids of Acholeplasma laidlawii strain B. 1. The phosphatidylglucose structure proposed previously (Smith & Henrikson, 1965) for the glucose-containing phospholipid from Acholeplasma laidlawii is incorrect. 2. The structure now proposed is 3-(sn-glycerol-3-phosphoryl-6'-[O-alpha-d-glucopyranosyl-(1-->2)-O-alpha-d-glucopyranosyl])- sn-1,2-diglyceride, a new type of bacterial lipid. 3. Deacylation of the lipid gave a single water-soluble phosphate ester which could be distinguished on chromatography from synthetic samples of glucosylphosphorylglycerols. 4. Hydrolysis of the lipid with alkali gave a mixture of fatty acids, glycerol 2-phosphate, sn-glycerol 3-phosphate and O-alpha-d-glucopyranosyl-(1-->2)-O-alpha- d-glucopyranosyl-(1-->1)-d-glycerol. 5. The lipid was unaffected on incubation with phospholipases A, C and D. 6. Diglucosyl diglyceride was isolated after treatment of the lipid with 60% HF, establishing the location of the fatty acid residues. 7. Periodate oxidation studies showed that the sn-glycerol 3-phosphate was esterified to the 6-hydroxyl group of one of the glucose residues in diglucosyl diglyceride.


The chemistry of the collagen cross-links. A convenient synthesis of the reduction products of several collagen cross-links and cross-link precursors. New synthetic routes to the reduction products of several collagen cross-links and cross-link precursors are described. By the use of these routes hydroxynorleucine, 5,6-dihydroxynorleucine, hydroxylysinonorleucine and hydroxylysinohydroxynorleucine can be synthesized via one common synthetic intermediate. The synthetic routes provide a convenient source of these unusual amino acids, as well as confirming the structure of hydroxylysinohydroxynorleucine and the other lysine-derived residues found in borohydride-reduced collagens.


Molecular structure of bacterial plasmids.


Effect of oxidative phosphorylation inhibitors on cyclic adenosine monophosphate synthesis in rat adipose tissue.


Biochemical lesion in rat liver mitochondria induced by hexachlorophene.


1-Ethyl-4-(isopropylidenehydrazino)-1H-pyrazolo-(3,4-b)-pyridine-5-carboxylic acid, ethyl ester, hydrochloride (SQ 20009)--a potent new inhibitor of cyclic 3',5'-nucleotide phosphodiesterases.


Metabolic action of urethan on protein synthesis and drug oxidation in normal and regenerating liver.


Control of prostaglandin biosynthesis in rat brain homogenates by adenine nucleotides.


Cyclic 3',5'-adenosine monophosphate response in the rabbit lung--adult properties and development.


NaF inhibition of phosphorylation and dephosphorylation involved in  -methyl-D glucoside transport in E. coli K 12. A pH dependant phenomenon sensitive to uncoupling agents.


On the determination of the molecular weight of lactate dehydrogenase by gel filtration on Sephadex G-200.


Inheritance of syndactyly.

Inheritance of syndactyly.


Follow-up of hydatidiform mole by radioimmunoassay of human chorionic gonadotrophin. An improved method for the follow-up of patients delivered of a hydatidiform mole using radioimmunoassay of human chorionic gonadotrophin is employed to ensure adequate sensitivity. Four illustrative case histories are described. Their HCG findings are presented to show that this is the principal basis on which decisions should be made.There are three aspects to the work of the laboratory. The technical work of the assay, the clerical effort of patient follow-up, and the clinical interpretation of results. The laboratory keeps a close check on follow-up (one of its most important functions) and receives specimens direct from patients. Overall control of all three aspects is by a clinician. It has been found useful to organize a special clinic for these patients to be seen. In this region it has been possible for this to be run by the same clinician.


Changes of blood pressure, renin, and angiotensin after bilateral nephrectomy in patients with chronic renal failure. Circulating levels of renin, angiotensin I, and angiotensin II were increased in six patients with chronic renal failure and hypertension uncontrolled by dialysis and hypotensive drugs. Lower and often normal levels were found in 10 patients whose blood pressure was controlled by dialysis treatment. For a variety of reasons all patients were subjected to bilateral nephrectomy. The logarithm of the decrease in plasma concentrations of renin and angiotensin II was significantly related to the fall of blood pressure after operation. Plasma renin concentration correlated significantly with blood angiotensin I concentration and with plasma angiotensin II in samples taken before and after nephrectomy. Renin, angiotensin I, and angiotensin II were measurable in samples of blood taken 48 hours or more after the operation.


Kinetic measurements with monocarboxylic acids as substrates and effectors of glutamate dehydrogenase.


Interaction of hydrogen peroxide with superoxide dismutase from erythrocytes.


Myeloproliferative disease in a turtle.

Myeloproliferative disease in a turtle.


Morbidity in post-myelogram patients--a survey of 100 patients.


Congenital syphilitic deafness.

Congenital syphilitic deafness.


Adolescent anorexia nervosa: the patient; an approach.


Rehabilitation and health visitor follow-up.


Instant pudding.

Instant pudding.


Trends in psychiatric care in Europe.

Trends in psychiatric care in Europe.


Nursing management of feelings, thoughts and behavior.


Hydrotherapy--is it all washed up?

Hydrotherapy--is it all washed up?


The financial plight of the elderly.

The financial plight of the elderly.


Malignant nonchromaffin paraganglioma.

Malignant nonchromaffin paraganglioma.


Electron paramagnetic resonance studies on the selenium-replaced derivatives of adrenodoxin: the presence of the one selenium-one sulfur compound.


Hormone-treated CHO cells exit the cell cycle in the G2 phase.


Cycl AMP in Gl-arrested BHK21 cells infected with adenovirus type 12.


The effect of hydration of lecithin-water lamellar phases: a comparative study of solute diffusion and ESR spectroscopic measurements.


Isolation and preliminary characterization of corticosterone-receptor complexes in mouse placental tissue.


Effect of glycolysis inhibitors on cyclic AMP synthesis in rat adipose tissue.


Partial purification and characterization of a renin-like enzyme from rat submandibular gland.


Effects of acute hypocapnia and hypercapnia on neuromuscular transmission and on monosynaptic spinal reflex in wakeful man.


The "wheat-stubble sarcoid granuloma": a new epithelioid granuloma of the skin.


Retrobulbar granular cell myoblastoma.

Retrobulbar granular cell myoblastoma.


Results of early mobilization and discharge after myocardial infarction. A total of 342 patients with acute myocardial infarction who were admitted to a coronary care unit are reviewed to assess the results of early mobilization and discharge. The mean duration of admission was 8.4 days and 89% of the survivors were discharged from hospital by the tenth day. The inpatient mortality was 15.5%. An additional 6.7% died during the six weeks' follow-up period, giving a total mortality of 22.2%. Altogether, 7.6% of patients were readmitted. Venous thromboembolic phenomena occurred in 3.5% during the inpatient period. Of patients who were eligible 62% were back at work five months after their myocardial infarction. We think the results justify a short hospital admission period for acute myocardial infarction.


Triglyceride storage disease: a disorder of lipolysis in adipose tissue in two patients. An obese patient was studied whose adipose tissue showed a significant reduction in release of glycerol (P < 0.05) when stimulated by isoprenaline despite normal rises in tissue levels of cyclic-AMP. However, lipolysis was stimulated by a fast of 14 days as judged by weight loss and a rise in plasma fatty acids from 0.4 to 1.2 mM. The defect in this patient may be familial since her obese daughter also showed diminished release of glycerol from adipose tissue on three occasions when stimulated by isoprenaline, despite normal rises in levels of cyclic-AMP.


Immunological studies in Ugandan patients with hepatocellular carcinoma. Immunological studies were performed on Ugandan patients with hepatocellular carcinoma to test the hypothesis that the high rate of persistence of hepatitis-associated antigen in these patients is the result of defects in host immune response. The responses to 1-chloro-2,4-dinitrobenzene sensitization and to a battery of recall skin test antigens were normal, as was the humoral antibody response to tularaemia antigen. Neither hypogammaglobulinaemia nor specific immunoglobulin deficiencies were found. Thus it appears unlikely that generalized defects in host immune responses can account for the high incidence of persistent hepatitis B virus infection found in Ugandan patients with hepatocellular carcinoma.


Zollinger-Ellison syndrome revisited.

Zollinger-Ellison syndrome revisited.


Where to die.

Where to die.


Monkeypox.

Monkeypox.


Four recurring themes.

Four recurring themes.


Ocular dyskinesia in cerebellar disease. Evidence for the similarity of opsoclonus, ocular dysmetria and flutter-like oscillations.


Hereditary neuropathy with liability to pressure palsies. Electrophysiological and histopathological aspects.


Purification and properties of rat liver nuclear protein kinases.


Lipid composition and synthesis in the muscles of normal and dystrophic chickens.


The amino acid sequence of cytochrome c-553 from the Chrysophycean alga Monochrysis lutheri.


Purification of isoenzyme and coenzymes for kinetic study of horse liver alcohol dehydrogenase.


Kinetic mechanism for the major isoenzyme of horse liver alcohol dehydrogenase.


Adenylate cyclase and insulin action. Effect of insulin, nonsuppressible insulin-like material, and diabetes on adenylate-cyclase activity in mouse liver.


Bicuculline and thalamic inhibition.

Bicuculline and thalamic inhibition.


Secondary antibody-forming potential of aged mice, with special reference to the influence of adjuvant on priming.


The activation of chloride transport by epinephrine and Db cyclic-AMP in the cornea of the rabbit.


Survival of Arthrobacter crystallopoietes during prolonged periods of extreme desiccation. Cells of Arthrobacter crystallopoietes mixed in sand and air-dried have survived for up to 6 months after an initial period in which approximately half the cells lost their viability. Comparative survival curves have been obtained from inoculated sands maintained under CaSO(4) or P(2)O(5). Selections for more desiccation-resistant progeny capable of surviving the initial period were unsuccessful. Both the coccoid and rod-shaped forms are equally resistant to several months of desiccation. Desiccated spherical cells converted 0.0005% of their cell carbon to carbon dioxide per hr, which corresponds to a half-life for self-consumption of approximately 12 years.


Ultraviolet-sensitive mutator strain of Escherichia coli K-12. An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4 on the chromosome. The mutator gene did not increase mutation frequencies in virulent phages or in lytically grown phage lambda. The mutU4 mutation predominantly induced transitional base changes. Mutator strains were normal for recombination and host-cell reactivation of UV-irradiated phage T1. They were normally resistant to methyl methanesulfonate and were slightly more sensitive to gamma irradiation than Mut(+) strains. UV irradiation induced mutations in a mutU4 strain, and phage lambda was UV-inducible. Double mutants containing mutU4 and recA, B, or C were extremely sensitive to UV irradiation; a mutU4 uvrA6 double mutant was only slightly more sensitive than a uvrA6 strain. The mutU4 uvrA6 and mutU4 recA, B, or C double mutants had mutation rates similar to that of a mutU4 strain. Two UV-sensitive mutators, mut-9 and mut-10, isolated by Liberfarb and Bryson in E. coli B/UV, were found to be co-transducible with ilv in the same general region as mutU4.


Acute renal failure followed by protracted, slowly resolving chronic uremia.


Specialized subregions of the bifunctional hisB gene of Salmonella typhimurium. Forty-three hisB mutants of Salmonella typhimurium have been screened to determine the molecular size of the resulting histidinol phosphate phosphatase activity, one of the activities of a bifunctional enzyme produced by this gene which also controls imidazole glycerol phosphate dehydrase activity. Mutation in hisB can lead to the loss of both phosphatase and dehydrase activities, or only of dehydrase activity. Through the use of nonsense mutants lacking dehydrase activity, a distinct point of transition was detected near the middle of hisB at which a dramatic change occurs in the size of the phosphatase enzyme that is synthesized. A missense mutant with a lesion in this region has a high-molecular-weight enzyme which is eluted in the void volume of a Sephadex G-200 column. The enzyme from nonsense mutants near the transition point have molecular weights near 40,000. Even though the buffer conditions are designed to favor the stabilization of the high-molecular-weight form, some mutants have both high- and low-molecular-weight forms. The polypeptide chain specified by the operator proximal part of hisB is sufficient to allow the expression of phosphatase activity. The synthesis of substantially less than the complete product of hisB resulted in association into a form similar to the native enzyme which was found in the void volume of a Sephadex G-200 column.


Electron-microscope observations on the formation of the cytoplasmic lamellar inclusion bodies in murine pulmonary tumours induced in vitro.


Membrane depolarization, cyclic AMP and glycerol release by brown fat in vitro.


Inhibition of post-ganglionic motor transmission in vas deferens by indirectly acting sympathomimetic drugs. 1. Using field stimulation with short trains of pulses (< 10 per train), the post-ganglionic motor transmission in the mammalian vas deferens has been further analysed pharmacologically.2. In preparations taken from guinea-pigs, rats and rabbits the effects of the indirectly sympathomimetic drugs, tyramine and cocaine, could be explained entirely on the basis of the actions of released, endogenous noradrenaline.3. Tyramine produced a contraction in vasa taken from normal rats but not from normal guinea-pigs. The tyramine contraction was due to release of endogenous noradrenaline because it was not seen in preparations taken from reserpinized rats and because it was abolished in normal vasa by phenoxybenzamine or phentolamine, thus denying the supposed inaccessibility, to alpha-blockers, of the motor alpha-adrenoceptors activated by endogenous noradrenaline.4. Phenoxybenzamine or phentolamine failed to block post-ganglionic motor transmission in rat and in guinea-pig vasa.5. Tyramine strongly inhibited motor transmission in vasa taken from normal but not from reserpinized guinea-pigs.6. Tyramine produced inhibition of motor transmission in phenoxybenzamine-treated preparations taken from normal but not from reserpinized rats.7. Cocaine inhibited motor transmission in guinea-pig and in rat vasa. This effect was not due to a local anaesthetic or to a smooth-muscle depressant action because it did not occur in preparations taken from reserpinized animals.8. The inhibitory effect of tyramine or cocaine was not abolished by beta-adrenoceptor blockade with propranolol.9. Whereas reserpinization abolished the tyramine- and cocaine-inhibitions, it did not affect the inhibitory actions of noradrenaline or of PGE(2).10. Indomethacin and sodium meclofenamate, which suppress prostaglandin synthesis, did not affect the twitch-inhibiting actions of noradrenaline, tyramine or cocaine.11. These results provide further support for the conclusion that post-ganglionic motor transmission to the vas deferens is non-adrenergic in these species and assign to endogenously released noradrenaline an inhibitory role upon motor transmission.


Transmitter release from insect excitatory motor nerve terminals. 1. Intracellular and extracellular electrodes were used to study spontaneous and impulse-linked release of transmitter at locust retractor unguis nerve-muscle synapses.2. At most extracellular recording sites the amplitude distributions of the excitatory post-synaptic potentials (e.p.s.p.s) were apparently non-Poisson. However, interpretation of these amplitude distributions was complicated by the effect on the extracellular recordings of the complex structural organization of the retractor unguis nerve terminal with its spatially distinct transmitter release sites extending over distances of 15-30 mum.3. The spontaneous miniature excitatory post-synaptic potentials (min e.p.s.p.s) did not occur at random intervals, bursts of min e.p.s.p.s being frequently recorded. As a result the spontaneous release of transmitter rarely approximated a Poisson process.4. For a period of at least 390 msec following a conditioning nerve impulse a test e.p.s.p. was facilitated and the probability of spontaneous transmitter release was enhanced. A large primary phase of facilitation of impulse-linked and spontaneous release was invariably followed by one or more secondary phases of smaller magnitude.


Historical vignette. Calomel and the American medical sects during the nineteenth century.


Experience with radioisotope scanning of the pancreas.


Properties of a temperature sensitive plasmid in Citrobacter freundii.


Symptomatic hypoglycemia in adults: a protocol for clinical and laboratory evaluation.


Plasmodial ultrastructure of the myxomycete Physarum polycephalum.


Fine structural studies on digestion of bacterial species in the rumen ciliate, Entodinium caudatum.


Morphology and behaviour of cultured aëdes aegypti mosquito cells.


The fine structure of the human oocyte. I. Oval granulo-vesicular bodies.


Immunocytological observations on the localization of ACTH in the hypophysis of the dog.


Structural studies on the synapsis of the toad, Bufo arenarum, Hensel. I. Special forms of dendro-dendritic, dendro-somatic and axo-somatic contacts.


Mononucleosis with heterophil antibodies and EB virus infection. Acquisition by an elderly patient in hospital.


Serial lipid and lipoprotein responses to the American Heart Association fat-controlled diet.


Recurrent lacrimal gland tumor.

Recurrent lacrimal gland tumor.


Electron microscope study of the morphogenesis of a new canine herpesvirus in dog kidney cells.


Induction of DNase activity in monkey cell cultures infected by simian virus 40.


Effect of panosialin on myxoviruses.

Effect of panosialin on myxoviruses.


Some aspects of the morphogenesis of myxoma virus in vivo.


Viral replication in mouse macrophages.

Viral replication in mouse macrophages.


Role of spleen in production of virus induced interferon in rabbits. Brief report.


Comparison of SV 40 RNA from infected cells and SV 40 cRNA made by RNA polymerase from Escherichia coli.


Oestrogen metabolism in cultured human breast tumours. The interconversion of tritium labelled oestrone and oestradiol-17β has been investigated in human breast tumours maintained in organ culture for 3 days. Benign tumours were significantly different from scirrhous carcinomata both in the concentration of radioactivity taken up by the tissue and in the ratios of oestradiol-17β/oestrone achieved. The fact that malignant tumours were able to convert oestrone to oestradiol-17β is of interest in view of the relatively high plasma levels of oestrone in post-menopausal women.


Why women form their own professional organizations.


M.D.? Mrs.?--PM&R.

M.D.? Mrs.?--PM&R.


Rehabilitation in modern medicine.

Rehabilitation in modern medicine.


The incidence and curability of cancer of the uterine cervix in El Paso County 1944-1967.


Characteristics of fast feedback control of corticotrophin release by corticosteroids.


A chemostat study of the effect of fixed nitrogen sources on nitrogen fixation, membranes and free amino acids in Azotobacter chroococcum.


The polar lipids of some species of Nocardia.


Chemodectoma of larynx.

Chemodectoma of larynx.


The ultrastructure of chemodectoma of the larynx.


[Radioimmunoassay of alpha-fetoprotein in primary and secondary cancer of the liver].


Chronic poliovirus infection of cocultivated monkey cells harboring the Mason-Pfizer monkey virus.


Cell-mediated tumor immunity measured in vitro and in vivo with soluble tumor-specific antigens.


Mammary gland tumors in substrains C57BL-M and C57BL-He mice.


Carcinoembryonic antigen: synthesis by a continuous line of adenocarcinoma cells.


Growth of Rous sarcomas in bursectomized chickens.


An anemia-inducing virus derived from tumors caused by murine sarcoma virus--Moloney.


Differential enhancement of R-type virus particles in polyoma-transformed BHK-21 cells by dimethyl sulfoxide.


Spontaneous disseminated squirrel fibroma.


Siegler R:Proposed classification of the murine leukemia-sarcoma viruses.


The mechanism of enhanced clearance of colloidal carbon from the blood of rabbits stimulated with a tubercle bacillary lipid.


Bovine respiratory syncytial virus. Studies on an outbreak in Japan, 1968-1969.


Distribution of antibodies in dogs against adenosatellite viruses.


Distribution of complement-requiring neutralizing antibody against herpes simplex virus in human population.


Sphingolipids of the fungi Phycomycetes blakesleeanus and Fusarium lini.


Effects of parathion on lipolysis in isolated rat adipose tissue cells.


Benign and malignant mixed tumours of the lung.


Elephantiasis and silicates.

Elephantiasis and silicates.


Ultrastructural changes in the rat liver following protracted treatment with pregnenolone-16 -carbonitrile and digitoxin.


Nucleoside triphosphatase activity in induced mammary tumours in rats.


Open tubular heterogeneous enzyme reactors: preparation and kinetic behavior.


A survey of the viruses of farm animals in Northern Ireland.


An abnormality of adrenaline, phentolamine stimulated lipolysis in adipose tissue from obese, maturity-onset diabetics.


The mechanism of insulin secretion after oral glucose administration. 3. Investigations on the mechanism of a reflectoric insulin mobilization after oral stimulation.


Retinal fine structure after long term anaesthesia.


The concentration of lactic acid in rabbit lenses of various ages.


The effect of cyclic AMP on the production of single-strand breaks in DNA of x-irradiated E. coli B-r.


8-Azaguanine resistance in mammalian cells. I. Hypoxanthine-guanine phosphoribosyltransferase. Chinese hamster cells were treated with ethyl methanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine, and mutants resistant to 8-azaguanine were selected and characterized. Hypoxanthine-guanine phosphoribosyltransferase activity of sixteen mutants is extremely negative, making them suitable for reversion to HGPRTase(+). Ten of the extremely negative mutants revert at a frequency higher than 10(-7) suggesting their point mutational character. The remaining mutants have demonstrable HGPRTase activity and are not useful for reversion analysis. Five of these mutants have < 2% HGPRTase and are presumably also HGPRTase point mutants. The remaining 14 mutants utilize exogenous hypoxanthine for nucleic acid synthesis poorly, and possess 20-150% of wild-type HGPRTase activity in in vitro. Their mechanism of 8-azaguanine resistance is not yet defined.


Ultrastructural examination of ageing and radiation-induced life-span shortening in adult Drosophila melanogaster.


Comparative effects of electron and neutron irradiation on the release of enzymes from isolated rat-spleen lysosomes.


The interaction of serum and arterial lipoproteins with elastin of the arterial intima and its role in the lipid accumulation in atherosclerotic plaques. Arterial elastin appears to be a proteinlipid complex with the lipid component being bound to elastin peptide groups. In atherosclerotic lesions the lipid content of elastin increases progressively with increasing severity of atherosclerosis. The increases in the lipid content of plaque elastin are mainly due to large increases in cholesterol with about 80% of the cholesterol being cholesterol ester. This deposition of cholesterol in elastin accounts for a substantial part of the total cholesterol accumulation in atherosclerotic lesions of all stages. The present in vitro study suggests that the mechanism involved in the deposition of lipids in arterial elastin may be an interaction of the elastin protein with serum or arterial low density or very low density lipoproteins (LDL and VLDL) resulting in a transfer of lipids, but not of lipoprotein protein to the elastin. No significant lipid transfer occurred from the high density lipoproteins or chylomicrons. The amount of lipid taken up by plaque elastin was strikingly higher than by normal elastin and consisted mainly of cholesterol with over 80% of the cholesterol being cholesterol ester. The precondition for the lipid accumulation in plaque elastin appeared to be an altered amino acid composition of the elastin protein consisting of an increase in polar amino acids and a reduction in cross-linking amino acids. Subsequent treatment of lipoprotein-incubated arterial elastin with hot alkali and apolipoproteins did not reverse the binding of lipoprotein lipid to diseased elastin.


Rifampicin, an antitrachoma antibiotic.

Rifampicin, an antitrachoma antibiotic.


Direct measurement of osteolysis in man. Precise, direct measurement of bone calcium release (v(o-)) has been accomplished using a continuous tracer administration (CTA) technique. Dietary calcium (96.97% (40)Ca) is replaced by (40)Ca (99.991% (40)Ca) and blood levels of the naturally occuring isotope (48)Ca are monitored by neutron activation analysis as a function of time. (48)Ca abundance falls as this isotope is excreted and only partially replaced by release from bone. After a suitable period, an asymptotic abundance of (48)Ca in serum, E, is approached which is the fraction of the turnover rate of the rapidly exchangeable calcium pools coming from the skeleton (E = v(o-)/v(t)). E is determined with a standard error of 2%, providing a precise, sensitive index of v(o-). 13 studies in three normal men and one postmenopausal woman receiving maintenance estrogen show large intersubject variations in parameters of calcium metabolism using both CTA and pulse tracer administration (PTA) plus balance techniques. Induced hypercalcemia results in a prolonged decrease in v(o-). Glucocorticoid therapy initially and consistently induces a marked hypercalciuria while effects on most other parameters of calcium kinetics are variable. In two men E fell when testosterone was added to glucocorticoid treatment, consistent with the known antiosteolytic effect of androgens, despite the short period of study.


Mechanism of the lower esophageal sphincter relaxation. Action of prostaglandin E 1  and theophylline. The intravenous injection of prostaglandin E(1) (PGE(1)) causes a dose-dependent relaxation of the lower esophageal sphincter (LES) in the intact, lightly anesthetized opossum. The action of PGE(1) is not inhibited by the drugs that produce muscarinic or nicotinic cholinergic antagonism or alpha and beta adrenergic antagonism in the doses that inhibited the action of respective agonists. Moreover, this action is not affected by exogenous gastrin pentapeptide. The action of PGE(1) on the LES is mimicked by isoproterenol, theophylline ethylenediamine, and dibutyryl cyclic AMP. Both theophylline, a phosphodiesterase inhibitor, and isoproterenol, an adenyl cyclase stimulator, added to the action of PGE(1). On the other hand, adenyl cyclase inhibitor nicotinic acid, as well as phosphodiesterase stimulator, imidazole inhibited its action. Further, both nicotinic acid and imidazole inhibited the degree of LES relaxation produced by esophageal distension. These studies suggest that intracellular cyclic AMP may act as the "second messenger" in the regulation of the lower esophageal sphincter relaxation.


Activation of renal cortical adenylate cyclase by circulating immunoreactive parathyroid hormone fragments. Three distinct immunoreactive species of parathyroid hormone (PTH) are present in human serum. One has an estimated mol wt of 9,500 and probably represents glandular hormone, the second 7,000-7,500 mol wt, and the third 4,500-5,000 mol wt. In order to assess the biological activity of these circulating forms of PTH, we determined their ability to activate renal cortical adenylate cyclase. The 9,500 mol wt and 4,500-5,000 mol wt fractions produced four- to sixfold increases in cyclic 3',5'-AMP accumulation above control; the 7,000-7,500 mol wt fraction was inactive. None of the fragments had any effects on phosphodiesterase activity. Antiserum to bovine PTH did not block the activation of adenylate cyclase by either the gragments or bovine PTH. The data suggest that a large proportion of circulating immunoreactive human PTH is biologically active and that the biologically and immunologically active sites of the hormone are distinct.


Studies on respiratory immunization with tetanus toxoid: the role of adjuvants. Aerosol vaccination of mice with purified plain tetanus toxoid does not induce an immune response unless a suitable adjuvant is added.Aluminium phosphate is without effect by aerosol treatment. Killed cells of Klebsiella pneumoniae, although effective, are unsatisfactory owing to the long inhalation period needed.Killed Bordetella perussis cells were found to be an excellent adjuvant. A single aerosol treatment with a toxoid-B. pertussis mixture during a moderate exposure period evoked a considerable immune response. With repeated aerosol treatment of primed mice the addition of adjuvant is not required; booster treatment with plain toxoid is at least as effective.Extracts from B. pertussis cells exert as good an adjuvant effect as the whole-cell vaccine. The remaining cell-wall debris also appears to be an active adjuvant.In combination with constant doses of adjuvant (10(8)B. pertussis cells), the 50% protective doses (ED 50) of toxoid were determined by inhalation and by s.c. injection and were found to be 0.1875 and 0.0625 LFU respectively. This would imply that, as a result of the adjuvant action, the s.c. ED 50 is reduced by approximately a factor of 20; whereas the respiratory ED 50 is decreased by at least a factor of 100.It is suggested that the much more pronounced adjuvant activity in aerosol immunization is associated with the induction of strong cell-mediated hypersensitivity in the respiratory tract.


Serological studies in a student population prone to infection with human papilloma virus. Four hundred and sixty-seven serum specimens from the female students at a residential college were examined for the presence of circulating antibody to human wart virus using the technique of counter-current immunoelectroosmophoresis. A significantly higher incidence of antibodies was found in students with a past history of plantar warts than in any other group. Antibody took several months to develop and was detectable in 20-30% of the students up to 9 years after infection. From a few cases of multiple infection, it was shown that reinfection could occur in spite of the presence of circulating antibodies probably of the IgG class. The sensitivity of the test was compared with two recognized techniques for detection of wart virus antibodies, namely gel diffusion and passive haemagglutination.


Passive protection of mice against intracerebral infections with Bordetella pertussis. The passive protection of mice against an intracerebral infection with Bordetella pertussis, by antiserum introduced directly into the brain with the infecting organisms, was compared with the protection afforded by intraperitoneal antiserum. The antibody effective by the intracerebral route is that which is adsorbed onto the infecting organisms, although it does not affect the viability of the organisms in vitro in the absence of complement.PASSIVE PROTECTION AGAINST ORGANISMS INTRODUCED INTRACEREBRALLY TAKES PLACE IN ONE OF TWO WAYS, DEPENDING ON THE SIZE OF THE CHALLENGE: (1) after 3-4 days' growth, the growth rate declines so that the number of organisms does not reach the figure lethal for the mouse; (2) the organisms do not appear to multiply, as their numbers decline from the moment of injection, so that the brain is sterile after 2 days.Many of the mice protected against a challenge of 50,000 organisms (ca. 100 LD 50) by intraperitoneal or intracerebral antiserum recover by the first mechanism. The second mechanism operates after a smaller challenge of 5000 organisms (ca. 10 LD 50), irrespective of whether the antiserum is given intracerebrally with the challenge, or intraperitoneally within several hours of challenge. Too much antiserum given intracerebrally with a 50,000 challenge, but not with a 5000 challenge, inhibits protection.In some mice, virulent antibody-treated organisms which have not been killed, grow very slowly over a long period, but are eventually eliminated from the brain.


The effect of the antibiotics distamycin A and congocidine on the replication of herpes simplex virus.


Routine administration of oral polio vaccine in a subtropical area. Factors possibly influencing sero-conversion rates. Poliomyelitis is an important problem of public health in warm-climate countries. Studies of serological responses to vaccination in these countries have given conflicting results but in many investigations the rates have been considerably less than in countries with temperate climates. In this study three possible factors influencing sero-conversion were investigated - the season of the year when vaccine was given, the social status of the mother (as indicated by the number of years of schooling) and the presence of non-poliomyelitis viruses (NPV) in the gut when vaccine was given.Over 200 children about 2 months of age were included in the study. Each was given three doses of trivalent vaccine at 6-week intervals.The sero-conversion rates of the groups fed in winter were excellent but were slightly less good in summer. The differences were greatest in children in the lower socio-economic groups and in children excreting other enteroviruses.The conclusions are that, provided a potent vaccine is used, the factors which diminish the effectiveness of immunization in warm-climate countries can be overcome: (1) by giving three doses of trivalent vaccine; (2) by beginning vaccination at the earliest possible age (when enteroviruses are fewest); (3) by concentrating special attention on the lower socio-economic groups and if necessary by giving a reinforcing dose several months after the third dose has been given - preferably in the colder months.


Filamentous particles in human alveolar cell carcinomas: electron microscopy studies of six cases (preliminary report).


The natural history and diagnosis of herpes cornea.


Studies on the molecular biology of herpes simplex virus.


Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies.


Myoepithelioma with a leukemoid reaction in BALB-c mice.


Isolation and properties of cells releasing C-type particles from BALB-c embryo cell cultures.


Collagenolytic enzymes in corneal pathology.


Fate of chromatin of interphase nuclei subjected to "phosphasing" in virus-fused cells.


Impaired inflammatory response in tumor-bearing guinea pigs.


Virus-specific nucleic acids in simian virus 40-transformed hamster cell clones varying in oncogenic potential.


Feline malignant mammary tumors. I. Morphology and biology: some comparisons with human and canine mammary carcinomas.


Polygraphic sleep study in craniopagus twins (where is the sleep transmitter?). By means of polygraphic recordings complete independence of the sequence of sleeping and waking and of the two sleep states (REM and NREM) could be demonstrated in twins extensively conjoined at the skulls. Functionally important connections in the circulation of the two babies were found during subsequent preoperative investigations. These observations are at variance with the hypothesis of an extraneuronal humoral sleep-inducing factor based on crossed-circulation experiments in animals; moreover they raise a new question about the transportation of the sleepregulating biogenic amines.


Interval analysis of repetitive denervation potentials of human skeletal muscle. Interval analyses of denervation potentials (fibrillation-potentials and positive sharp waves) in human skeletal muscles were performed using a DEC PDP-12 computer. Extremely small differences between consecutive intervals (usually less than 1%) and slow up and down drifts in the spontaneous discharge frequency were found to be characteristic of repetitive denervation potentials. These two findings are safe criteria for the clinical diagnosis of denervation and they may be superior to the more commonly used criteria based upon the shapes, amplitudes, and durations of the potentials. Physiological data from the literature as well as hypotheses relating to spontaneous activity are reviewed. Generator potentials across the muscle fibre membrane, lowered firing threshold, and constant-sized after-potentials are considered the basic factors relevant to spontaneous and rhythmical firing and to the slow drift observed in the firing frequency.


Interaction between voluntary contraction and tonic stretch reflex transmission in normal and spastic patients. Transmission characteristics of tonic stretch reflex (TSR) pathways in both normal and spastic patients have been measured during different levels of sustained voluntary contraction of the biceps brachii muscle. A cross-correlation technique of analysis was used to separate reflex responses from the total electromyographic activity. TSR transmission in normal subjects was observed to change with the level of voluntary contraction; sensitivity to stretch increased approximately three-fold as the subject stiffened the arm by simultaneously contracting flexor and extensor muscles. In contrast, TSR transmission did not alter during voluntary contraction in spastic patients. It is proposed that in spastic patients supraspinal modulation of reflex transmission is impaired because hypersensitive spinal reflexes short-circuit long loop pathways.


Nerve potentials and afferent conduction velocities in the differential diagnosis of amyotrophy of the hand. Nerve action potentials recorded over the elbow after stimulation of the wrist were studied in patients with hand amyotrophy of different origin. In amyotrophic lateral sclerosis the amplitude of nerve action potentials does not change significantly even with extreme muscle wasting and loss of motor units.Studies of multisegmental afferent conduction velocities may demonstrate a strictly localized lesion with normal values above the compression.


Corneal reflex in hemisphere disease.

Corneal reflex in hemisphere disease. The contralateral corneal reflex may be absent in patients with a deep lesion of the parietal lobe. Frontal and temporal lobe lesions apparently do not interfere with this reflex.


Subacute spongiform encephalopathy. Electron microscopic studies. Electron microscopic changes in brain biopsies from two cases of subacute spongiform encephalopathy are described. Large vacuoles seen in astrocytic and neuronal processes were surrounded by one or more concentric membranes. Dense-core vesicles were found in axonal endings and in nerve cell perikarya; these particles could represent catecholamine-containing vesicles.


A method for the quantitative determination of urinary estriol conjugates in human pregnancy.


Possible mechanisms of anesthesia.

Possible mechanisms of anesthesia.


Alpha-fetoprotein as an aid for diagnosis of primary hepatoma.


Requirement of proteins S5 and S9 from 30S subunits for the ribosome-dependent GTPase activity of elongation factor G. During CsCl isopycnic centrifugation at 20 mM Mg(++), Escherichia coli 30S ribosomal subunits specifically lose proteins S1, S2, S3, S5, S9, S10, and S14. The resultant 30S core is unable to stimulate the GTPase activity of EF-G in the presence of 50S subunits. Activity could be restored to a small extent by adding back S2, S5, or S9. However, when S5 and S9 were added together, they cooperatively produced 30S particles 1.5 times more active than the original native 30S subunits. The small amount of activity restored by S2 was simply additive to that restored by S5 or S9. None of the other split proteins showed any restoring capability. Ability of the various protein-deficient 30S particles to couple with 50S subunits corresponded closely to their activity in the EF-G GTPase reaction. It is concluded that S5 and S9 together enable the 30S subunit to participate in the formation of a GTPase-active 30S-50S-EF-G complex.


Malignant lymphomas of African children. Clinical, pathologic, and epidemiologic observations of malignant tumors of children in sub-Sahara Africa suggest alternative theories of causation, and give insight into environmental influences that may play a large role in the etiology and form of malignant lymphatic tumors and cerebral neoplasms of infants.


Prevention of simian virus 40 tumors by hamster fetal tissue: influence of parity status of donor females on immunogenicity of fetal tissue and on immune cell cytotoxicity. Fetal tissue from primiparous hamsters prevented simian virus 40 (SV40) tumorigenesis in male hamsters, whereas fetal tissue from multiparous hamsters did not. The parity status of normal (uninoculated) hamsters also influenced the cytotoxicity of their lymphoid cells against tumor cells. Lymph node cells from nonpregnant primiparous and multiparous animals were cytotoxic in microcytotoxicity tests against SV40, polyoma, and adenovirus 7 tumor cells, but were not active against control BHK cells. Lymph node cells from virgin female donors were inactive. Peritoneal exudate cells from these donors reacted in similar fashion against SV40 tumor cells in vitro and in adoptive transfer tests in vivo. However, the cytotoxicity of peritoneal exudate cells from multiparous hamsters was greatly reduced during pregnancy, a time when noncytotoxic humoral antibody reactive with surface antigen of SV40 tumor cells is present. This humoral antibody is not detected during first pregnancy, and peritoneal exudate cells obtained from pregnant primiparous hamsters demonstrated a high degree of cytotoxicity.


Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Marmoset blood leukocytes transformed in vitro by Epstein-Barr virus regularly release extracellular infectious Epstein-Barr virus with high titers of transforming activity. By comparison, human umbilical cord leukocytes and adult human leukocytes transformed by Epstein-Barr virus release either no extracellular infectious virus or small amounts, irregularly.


Mutagenic DNA polymerase in human leukemic cells. Evidence is presented that DNA polymerases from human leukemic cells are mutagenic. Nucleic acid-free extracts of acute lymphoblastic leukemic cells polymerized about 10-times more dCTP using poly(dA-dT).poly(dA-dT) as a template than did extracts from normal lymphocytes. Mutagenic DNA polymerases could perhaps play an important role in tumor progression.


Double-stranded RNA as an inhibitor of protein synthesis and as a substrate for a nuclease in extracts of Krebs II ascites cells. Concentrations of double-stranded RNA above about 0.1 mug/ml inhibit translation of encephalo-myocarditis viral RNA and mouse globin messenger RNA in extracts of Krebs II ascites cells. Protein synthesis initially proceeds at the control rate, then abruptly shuts off in a manner similar to that observed in reticulocyte lysates [Hunt, T. & Ehrenfeld, E. (1971) Nature New Biol. 230, 91-94]. Substantially higher concentrations of double-stranded RNA are required to give this effect in ascites extracts. Subcellular fractions of Krebs II ascites cells contain a nucleolytic activity capable of digesting several natural and synthetic double-stranded RNAs. This nuclease is most active under conditions of protein synthesis, and part of the activity remains associated with ribosomes upon sedimentation. It is probably because of digestion of double-stranded RNA by this nuclease that higher concentrations of double-stranded RNA are required for inhibition of protein synthesis in Krebs cell extracts than in reticulocyte lysates.


Structural characteristics of some murine RNA tumor viruses studied by lactoperoxidase iodination. Iodination by the noninvasive enzymatic lactoperoxidase technique has been used to study the enzyme-accessible and enzyme-inaccessible proteins of three oncorna viruses (radiation leukemia virus, Moloney leukemia virus, and mouse mammary tumor virus). The number and relative molecular weight of proteins associated with virion preparations purified on sucrose gradients were characterized by scans of Coomassie blue-stained bands after dodecyl sulfate-polyacrylamide gel electrophoresis. Gel scans from the leukemia viruses are similar, each showing six distinct major protein bands on stained gels. The mammary tumor virus proteins by this analysis are not similar to those of the leukemia viruses. Enzymatic iodination of intact virion preparations led to the solitary labeling of one of the major proteins of each virus-the 80,000-dalton protein of the leukemia viruses and the 52,500-dalton protein of the mammary tumor virus. These are tentatively positioned as surface (enzyme-accessible) moieties in the virions. Disruption of each virus with a nonionic nonionic detergent before enzymatic iodination led to the labeling of the remaining stainable bands. The four lower molecular weight bands of the leukemia viruses are tentatively positioned as internal (enzyme-inaccessible) components.


Malignant transformation of BHK21 clone 13 cells in vitro by nitrosamines--a conditional state. All the BHK(21) Clone 13 cells transformed by dimethylnitrosamine or nitrosomethylurea, and a single "spontaneously" transformed clone obtained in our laboratory, exhibit a conditional state of the transformed phenotype. Upon growth at 38.5 degrees , they have a transformed phenotype (clonal morphology and the ability to plate in soft agar); when grown at 32 degrees , their phenotype is normal. The conditional state of these cells, however, does not extend to their growth characteristics; their plating efficiency at the two temperatures in liquid medium is similar. Conversion from the normal to the transformed phenotype and the reverse is possible by temperature shifts. A working hypothesis is proposed suggesting that this phenomenon may be caused by a temperature-sensitive mutation of a cellular gene whose product is responsible for maintaining the normal phenotype. The high temperature would then affect either the synthesis or the function of the protein carrying the temperature-sensitive alteration and, as a consequence, the transformed phenotype would be expressed.


Production of mucopolysaccharides by normal and transformed cells. The rate of hyaluronic acid and sulfated mucopolysaccharide production was measured for hamster embryo fibroblasts and for general oncogenic lines derived by virus transformation. A striking increase in both the rate of hyaluronic acid synthesis and the amount of cell-associated polymer was observed after transformation by herpes simplex type-2 or SV40 virus. Although no corresponding change was observed for the sulfated polysaccharides, the proportion of heparan sulfate increased significantly after transformation.


RNA related to that of a murine leukemia virus in Burkitt's tumors and nasopharyngeal carcinomas. RNA homologous to that of the Rauscher leukemia virus has been detected in Burkitt's lymphomas and nasopharyngeal carcinomas. Earlier excellent experimental evidence has linked these two human tumors with the Epstein-Barr virus, a DNA-containing agent.


Electron spin resonance studies of spin-labeled mammalian cells by detection of surface-membrane signals. Lipid-soluble spin labels were incorporated into human lymphocytes and mouse L-cells and the resulting electron spin resonance spectra were compared with spectra obtained from similarly labeled human erythrocytes. Spin labels were found in all subcellular fractions of the nucleated cells that contained membranes. Spinlabeled cells remained viable and capable of replicating in vitro. Electron spin resonance signals from spin-labeled nucleated cells underwent a time- and temperature-dependent decay that was reversed by bathing the cells in K(3)Fe(CN)(6). The demonstration of a relative cell impermeability to ferricyanide, as measured by both colorimetric and radioisotopic label methods, indicated that only spin-labeled molecules in the surface membrane were reactivated when ferricyanide was added to spin-labeled cells after the electron spin resonance signal had decayed.


Induction of cytolysis of cultured lymphoma cells by adenosine 3':5'-cyclic monophosphate and the isolation of resistant variants. Cultured mouse lymphosarcoma cells are killed on exposure to 0.1 mM N(6),O(2')-dibutyryl-adenosine 3':5'-cyclic monophosphate. A population of cells resistant to the killing effect of dibutyryl cyclic AMP at concentrations as high as 1 mM was selected. The growth characteristics of the resistant cells were similar to those of the sensitive parental line. However, the resistant cells contain less cytoplasmic cyclic AMP-binding proteins and decreased cyclic AMP-stimulated protein kinase activity. It is proposed that transition from sensitivity to resistance to dibutyryl cyclic AMP in lymphoma cells is connected with a modification of the cyclic AMP-binding protein, which appears to be the regulatory subunit of the cyclic AMP-activated protein kinase.


Pharmacology of the brain: the hippocampus, learning and seizures.


Adenosine 3',5'-monophosphate: electrophysiological evidence for a role in synaptic transmission. Synaptic potentials and changes in resting membrane potentials of superior cervical ganglia of the rabbit were measured in the presence of adenosine 3',5'-monophosphate and agents that affect its metabolism. Adenosine 3',5'-monophosphate and its mono- and dibutyryl derivatives caused a hyperpolarization of the postganglionic neurons. Theophylline potentiated the slow inhibitory postsynaptic potential that follows synaptic transmission, as well as the hyperpolarization of postganglionic neurons caused by exogenous dopamine. Conversely, prostaglandin E(1) inhibited both the slow inhibitory postsynaptic potential and the dopamine-induced hyperpolarization. We hypothesize that the slow inhibitory postsynaptic potential as well as the dopamine-induced hyperpolarization result from increased amounts of adenosine 3'5'-monophosphate in the postganglionic neurons. The dibutyryl derivative of guanosine 3'5'-monophosphate caused a depolarization of the postganglionic neurons, which is consistent with the possibility that guanosine 3'5'-monophosphate mediates synaptic transmission at muscarinic cholinergic synapses.


Anaesthesia of fish.

Anaesthesia of fish.


Amounts of gonadotrophins required for normal follicular growth in hypophysectomized adult rats.


Effect of progesterone on the time of ovulation in immature rats pretreated with pregnant mare's serum gonadotrophin (PMSG). 21-day-old female rats maintained under 14 hours light and 10 hours darkness were used to determine if there is any effect of progesterone on the time of ovulation, time of release of pituitary gonadotropins (critical period), and the change  in plasma LH levels in immature rats pretreated with pregnant mare's serum gonadotropin (PMSG).  3 OR 12 IU PMSG was injected on Day 22, 7:00 - 8:00 A.M. Colony Time.  3 IU PMSG-treated rats were injected at 10:00 A.M. Day 24 with either .5 mg progesterone or 10 mcg estrone.  2 groups of 3 IU PMSG rats were given .5 or 2 mg progesterone at 5:30 A.M., Day 24.  Time of ovulation was determined by sacrificing rats every 30 minutes from midnight - 4:00 A.M. and at 8:00 A.M. on Day 25.  In 2 experiments rats primed with 3 or 12 IU PMSG were injected with 2 mg progesterone at 5:30 A.M. on Day 24 followed by 4.5 mg phenobarbital from 5:30 - 4:00 P.M.  Samples for LH assay were taken at 6:00 A.M., 10:00 A.M., 3:00 P.M., and 8:00 P.M. Day 24 and 9:00 A.M., Day 25.  23-day-old Holtzman rats were used for LH assay.  50 IU PMSG and 25 IU HCG were given between 10:00 and 11:00 A.M., Day 25, or 4:00 and 5:00 P.M., Day 27, respectively, with daily injections of 10 mcg estradiol-3-benzoate Days 30-38.  Assay was performed when rats were 39 days old by the method of bogdanove and Gay.  The concentration of ascorbin acid was determined by the method of Mindlin and Butler.  Duncan's multiple range was used to calculate significance.  Ovulation time of 3 IU PMSG-primed rats was between 1:00 and 4:00 A.M. on Day 25 as determined by presence of ova in the oviducts.  Time of ovulation was 1:00 - 2:00 A.M., Day 25, when .5 mg progesterone was given at 10:00 A.M., Day 24.  Ovulation occurred at 11:00 P.M., Day 24 when .5 or 2 mg progesterone was injected at 5:30 A.M., Day 24.  12 IU PMSG-treated rats had ovulation occur 10:00 P.M., Day 24 - 2:00 A.M., Day 25 when 2 mg progesterone was given 5:30 A.M., Day 24.  4.5 mg phenobarbital given at 10:30 or 11:00 A.M. Day 24 in rats treated with 3 or 12 IU PMSG respectively inhibited ovulation, indicating the critical period had been shifted forward by 3 hours.  Highest level of LH was present in 3:00 and 8:00 P.M. plasma, Day 24 in rats primed with 3 IU PMSG alone and at 10:00 A.M. and 3:00 P.M., Day 24 when progesterone was also given.  Progesterone advanced the time of ovulation by shifting the critical period and causing an adequate release of ovulatory gonadotropins 10:00 - 3:00 P.M. on the day preceding ovulation.


C 21  steroid metabolism and conjugation in the human premature neonate. 2. Tissue metabolism and conjugation of exogenous cortisol, 11-deoxycortisol, corticosterone and pregnenolone in the presence and absence of ACTH in vitro.


Imitation of the adreno-cortical response to surgery by intravenous infusion of synthetic human ACTH.


Reduction malformations and chromosome anomalies.


Undifferentiated lymphoma, non-Burkitt's type. Meningeal and bone marrow involvement in children.


Estrogen treatment of tall girls.

Estrogen treatment of tall girls.


Development of hip dysplasia in dogs.

Development of hip dysplasia in dogs.


Mechanisms of platelet function as revealed by the retention of platelets in glass bead columns.


Adhesion of platelets to subendothelium.


The control of platelet responsiveness by agents that influence cyclic AMP metabolism.


Psammoma bodies found in cervico-vaginal smears.


Cyclic 3',5'-adenosine monophosphate in human blood platelets. IV. Regulatory role of cyclic amp in platelet function. Evidence which bears on the hypothesis that agents that increase platelet cyclic adenosine 3',5' monophosphate (cAMP) tend to inhibit platelet aggregation, whereas agents whose action is to induce or augment platelet aggregation are associated with a decrease in platelet content of this cyclic nucleotide is reviewed.  Using radiolabeled cAMP in incubation with various agents, it was found that caffeine, colagen, thrombin, prostaglandins E1 and E2, and phosphodiesterase inhibitors are all agents that increase platelet cAMP and inhibit platelet aggregation.


Purification and properties of cytochrome c (550: Hemispora stellata).


Rapid single-step kinetic colorimetric assay for lactate dehydrogenase in serum.


Polyacrylamide-gel disc-electrophoresis as a screening procedure for serum lipoprotein abnormalities.


Polyacrylamide-gel block-electrophoresis of plasma lipoproteins.


Automated kinetic spectrophotometric assays of enzyme activities of human cerebrospinal fluid: methods and reference values.


Additional observations on hydrochlorothiazide interference with measurement of total urinary placental estrogens.


Identification of lithocholic acid and measurement of other bile acids in serum of healthy humans.


Enzyme determinations: criticisms of some recent reports.


The lipid composition of the larval nervous system of Musca domestica. A comparison between insects susceptible and resistant to cyclodiene insecticides.


Experimental ocular studies with polyinosinic acid: polycytidylic acid.


Subcellular localization of avian leucosis group-specific complement-fixing antigens in mouse ascites sarcoma cells (SR-C3H-He cells) induced by Schmidt-Ruppin strain of Rous sarcoma virus.


Mitochondrial oxidation of 3,3'-diaminobenzidine and related compounds, and their possible relation to carcinogenesis.


Mechanism of antitumor action of dimethanesulfonylthiopentane.


Transplacental carcinogenesis by urethan in mice: teratogenesis and carcinogenesis in relation to organogenesis.


Immunohistological studies on mucosal glycoprotein in rectal carcinoma.


Hepatocarcinogenicity of polychlorinated biphenyls in mice.


Deviations in isozyme patterns of acid phosphatase and esterase, and in ultrastructures of Yoshida ascites hepatomas from rat hepatocytes.


Effects of crystalline environment on pyrimidine free-radical formation: barbital crystal polymorphs and sodium barbital.


Aquaculture and associated diseases of fish of public health importance.


Inhibition in vitro of cyclic 3',5' nucleotide phosphodiesterase activity by drugs.


Calcium-mediated effects of calcitonin on cyclic AMP formation and lymphoblast proliferation in thymocyte populations exposed to prostaglandin E 1 .


Ammonia effects in cultures of normal and transformed 3T3 cells.


Biochemical characteristics of normal and virally transformed mouse cell lines.


Induction of reagin synthesis in the rat with associated and dissociated hemocyanin: effect of antilymphocyte serum.


Infection of thymus cells in vivo and in vitro with a guinea pig herpes-like virus and the effect of antibody on virus replication in organ culture.


Proportion of T and B lymphocytes in lesions of Marek's disease: theoretical implications for pathogenesis.


In vitro assay of cellular immunity to tumor-specific antigen(s) of virus-induced tumors by macrophage migration inhibition.


Preliminary x-ray studies of a cytochrome c from Micrococcus dentrificans.


Circularity and cross-linking in preparations of replicative form of encephalomyocarditis virus RNA.


Cytokinesis in the stratum intermedium of the rat molar enamel organ.


Ultrastructural changes in Osmunda regalis Prothalli induced by X-irradiation.


Crystalline inclusion bodies in the epididymis of the nine-banded armadillo.


Acute herpetic gingivostomatitis in children.


Electron microscopy of artichoke mottled crinkle virus in leaves of Chenopodium quinoa Willd.


Contribution of polysynaptic pathways to the tonic vibration reflex.


The positive after-potential following the orthodromic and antidromic propagated impulses in the frog muscle spindle.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 8-1973.


Ski-boot neuropathy.

Ski-boot neuropathy.


West Germany's geriatric patients: community care and after-care.


Actions of adrenal, hypophysial and renal hormones on the renal excretion of water and electrolytes.


Effect of colchicine on the fine structure of the neuromuscular junction.


Nervous system of the snail Helix aspersa. 3. Electron microscopic study of neurosecretory nerves and endings in the ganglionic sheath.


Fine structure of the neural sheath, glia and neurons in the brain of the housefly, Musca domestica.


Studies of the fine structure of ovarian interstitial tissue. 7. The postnatal evolution of the thecal gland in the domestic fowl.


Intrinsic autoregulatory control of heart rate and its applied and clinical implications.


Neuro-epithelial bodies in the respiratory mucosa of various mammals. A light optical, histochemical and ultrastructural investigation.


Histochemical studies on the morphology of the Golgi apparatus and on the distribution of hexokinase and some enzymes concerning vitamin C synthesis in the substantia nigra of the rabbit.


The dynamics of histochemical changes in the epithelium of the small intestine of mice after experimental intraperitoneal intoxication with benzene.


The avian intestinal flora with particular reference to the possible ecological significance of the cecal anaerobic bacteria.


Home hemodialysis.

Home hemodialysis.


The etiology of respiratory tract infections in a tropical country.


Chlorpropamide in diabetes insipidus.

Chlorpropamide in diabetes insipidus.


The isolation and chemical composition of premelanosomes and melanosomes: human and mouse melanomas.


Abnormal tryptophan metabolism in patients with scurvy-type skin.


The group estimation of urinary 11-oxy-17-hydroxy-corticosteroids using gas-liquid chromatography.


Insulin from individual isolated mouse islets of Langerhans.


Atachment of normal and transformed hamster kidney cells to substrata varying in chemical composition.


Effects of angiotensin I and antiotensin II on canine hepatic vascular resistance.


Trypanosoma gambiense: cytopathologic changes in guinea pig hepatocytes.


Enzymes of coccidia: purification and properties of L-lactate dehydrogenase from Eimeria stiedae.


Comparative studies of lipid content and lipid metabolism of normal and transformed mouse cells.


Kinetics of incorporation of ( 32 P)phosphate into phospholipids of a SV 40 transformed mouse cell during logarithmic growth.


Glycolipids of mouse fibroblasts and virus transformed mouse cell lines.


Steroidglucuronyltransferases. 3. Oestradiol-17  3-glucuronyltransferase of the mitochondria of pig intestine.


Comparison of the biological, biophysical and antigenic properties of four strains of infectious bovine rhinotracheitis herpesvirus.


Steroidglucuronyltransferases. IV. Localization of the oestradiol-17 -glucuronyltransferase in the outer membrane of the mitochondria of pig intestine.


Pathogenesis of meningo-encephalitis produced in calves by infectious bovine rhinotracheitis herpesvirus.


Pathogenicity of Talfan and Konratice strains of Teschen virus in gnotobiotic pigs.


The Merkel cell.

The Merkel cell.


Infection of some species of African wild life with foot-and-mouth disease virus.


A preliminary crystallographic investigation of rice cytochrome c.


Effects of oral contraceptives on the renin-angiotensin system and on blood pressure of normal young women.


Epidemiology of acute lower respiratory disease in children.


Acute promyelocytic leukemia in pregnancy.


EEG delta activity during human sleep as a damped ultradian rhythm.


Haematemesis caused by oesophageal varices.


Diagnosis and treatment of hirsutism in women.


Serum lipoprotein patterns in Bantu and Indian patients with nephrotic syndrome.


Cyclic adenosine monophosphate and hypertension in rats. Aortas from spontaneously hypertensive and stress hypertensive rats contained significantly lower intracellular concentrations of cyclic adenosine monophosphate than did their respective controls. Adenylate cyclase activity was normal but was less responsive to stimulation, while phosphodiesterase activity (especially the low Michaelis-Menten constant form) was significantly elevated. Human aortas contained two forms of phosphodiesterase that were similar to those in rat aortas.


Virologic studies of generalized herpes zoster.


A psychophysiological study of nightmares and night terrors. The suppression of stage 4 night terrors with diazepam.


Determination of plasma renin activity by radioimmunoassay of angiotensin I.


Monkeypox virus (MPV). I. Propagation, purification, and further characterization.


Increase of plasma renin-substrate concentration after infusion of angiotensin in the rat.


Monitoring of neuromuscular transmission in anesthetized man by a bulb-transducer assembly.


Evidence for free radical involvement in the hydroxylation of proline: inhibition by nitro blue tetrazolium.


Computer evaluation to the x-ray densitometry method for the diagnosis of calcipenic osteopathy.


The responses in culture of human tumour astrocytes and neuroblasts to N 6 , O 2' -dibutyryl adenosine 3',5'-monophosphoric acid.


Thiamin deficiency: liver metabolite levels and redox and phosphorylation states in thiamin-deficient rats.


Malignant lymphoma and nephrotic syndrome. A clinicopathologic analysis with light, immunofluorescence, and electron microscopy of the renal lesions.


Vitamin A and bone metabolism in the rat.


Malignant mixed mullerian tumor of the cervix following cyclophosphamide therapy for nephrotic syndrome.


Hyperthyroidism induced by trophoblastic thyrotropin.


New drugs for treatment of Paget's disease.


Diplovax. A new oral polio vaccine.

Diplovax. A new oral polio vaccine.


Inactivation of Q beta RNA by electrophiles.


Genetical behaviour of vesicular stomatitis virus during successive passages at high and low temperatures.


Genetic analysis of replicating instabilities in yeast.


Cytogenetic effects of hycanthone in the rat.


Cytogenetic and mutagenic effects of DDT and DDE in a Chinese hamster cell line.


Demonstration by electron microscopy of viruses in cells found by light microscopy to contain inclusion bodies.


Long survival in a male with 18-trisomy syndrome and Wilms' tumor: a subsequent report.


Role of adenosine 3',5'-monophosphate (cyclic AMP) in actions of catecholamines.


Effect of angiotensin II on arterial blood pressure, glomerular filtration rate and urinary sodium excretion in normal, pregnant and hypertensive rats.


Percutaneous adrenal venography in the dog.


The all-ulnar hand.

The all-ulnar hand.


Parametric evaluation of the prevalence of Entamoeba histolytica infections in Ibadan.


The fine structure of the sporozoites of Isospora canis.


Ultrastructural study of microsporidian invasion into cells.


Study on parainfluenza virus type 1 isolated from pigs.


Vitamin D-resistant rickets and hemophilia A--lack of close linkage on the X chromosome.


The effects of gallamine on nerve terminals and endplates.


Variability of  -galactosidase A and B in different tissues of man.


The mechanism of inhibition by fluoride of mitochondrial fatty acid oxidation.


Cyclic 3',5'-adenosine monophosphate-dependent protein kinase of skin. I. Measurement and properties.


Cyclic 3',5'-adenosine monophosphate-dependent protein kinase of skin. II. Levels in atopic dermatitis and psoriasis.


Factors controlling the rate of fatty acid  -oxidation in rat liver mitochondria.


Virus studies in the treatment of congenital cytomegalovirus infections by cytosine arabinoside.


Determination of the molecular weight of human gamma-3 chains by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate.


The persistence of tissue-specific antigen in muscle cells growing in vitro.


The effect of anti-lymphocytic antibody on the humoral immune response in different strains of mice. I. The response to bovine serum albumin.


Adrenocorticotropin. 43. The synthesis and steroidogenic activity of (2-N-methylphenylalanine)-adrenocorticotropin-(1-19).


Studies on L-glycerol-3-phosphate dehydrogenases in Japanese quail, Coturnix coturnix japonica. I. Multiple components and some enzymatic properties of nicotinamide adenine dinucleotide-linked L-glycerol-3-phosphate dehydrogenase from liver and testes.


The purification and properties of geranylgeranyl pyrophosphate synthetase from pumpkin fruit.


Vertebrate collagenase: direct extraction from animal skin and human synovial membrane.


Studies on N-Acetyl- -D-glucosaminidase of Aspergillus oryzae. 3. Transglycosylation by the enzyme and preparation of crystalline phenyl N, N'-diacetyl- -chitobioside.


Reconstitution of hepatic microsomal stearoyl-coenzyme A desaturase system from solubilized components.


Acetanilide-hydrolyzing esterase of rat liver microsomes. I. Solubilization, purification, and intramicrosomal localization.


Enzyme kinetics and its relevance to enzyme assay.


A simple interferon assay as an adjunct for determining the genus of origin of cell cultures.


Dissociation of single cells from lung or kidney tissue with elastase.


The effect of neonatal and adult thymectomy on the inflammatory response.


The digestion of cellular fragments within phagolysosomes in carcinoma cells.


A comparative histochemical study of rat livers in alpha-naphthyl-iso-thiocyanate (ANIT) and DL-ethionine intoxication.


Prostaglandins and reproduction.

Prostaglandins and reproduction.


Multilocular renal cystic disease in children.


An approach to metastatic nephroblastoma.


The radiologic discrimination of abdominal masses in infants.


Localization of adrenal tumors by photoscanning.


Aspects of the encapsidation of simian virus 40 deoxyribonucleic acid. Growing subcloned CV1-cells were infected with simian virus 40, and the time course of virus formation was determined. When infected cells were fractionated into cytoplasmic and nuclear fractions, most of the progeny virus particles were recovered in the cytoplasmic extract and not in the nuclei. This result was independent of the technique used for the preparation of nuclei and of the time after infection at which the extracts were prepared. Leakage of the virions from the nucleus occurred during the course of cell fractionation, suggesting that the nuclear membrane of the infected cells is damaged. Virions were found to accumulate in a nonlinear fashion, at the time when the number of viral deoxyribonucleic acid (DNA) molecules increases linearly with time after infection. This suggests that the size of the intracellular pool of capsid proteins increases constantly during the late phase of virus replication. Progeny viral DNA to become encapsidated is withdrawn at random from the pool of replicated DNA molecules.


Viruses of Entamoeba histolytica. 3. Properties of the polyhedral virus of the HB-301 strain. Some biological characteristics and bioassay of a polyhedral virus isolated from normal-appearing strain HB-301 of Entamoeba histolytica are described. The virus produced lysis of susceptible strains of E. histolytica, yet it caused no cytopathological effect in the host strain, HB-301. The virus and host amoeba existed in an equilibrium; approximately 10(4) mean infective dose units of virus were produced per million HB-301 amoebae. Superinfection and antiviral antiserum treatment failed to disturb this equilibrium permanently. The mechanism of viral persistence in strain HB-301 amoebae remains to be determined. Purification of the virus was attempted. Ninety-nine percent of the viral infectivity was associated with a low-speed pellet which consisted of complexes of virus and cellular membranes. Various treatments of this low-speed pellet failed to release virus. Biologically active, membrane-free virus of low titer was prepared by differential centrifugation of supernatant solutions and employed in electron microscopy and other studies.


Molecular weights of vesicular stomatitis virus and its defective particles by laser light-scattering spectroscopy. Laser light-scattering spectroscopy has been used to determine the diffusion coefficients of vesicular stomatitis virus and three of its defective particles in order to calculate their molecular weights.


Replication of the Moloney murine sarcoma-leukemia virus in XC cells. The XC rat cell line was found to support the replication of a strain of the Moloney murine sarcoma-leukemia virus. In growth curve experiments cytopathology was paralleled by the production of murine sarcoma virus and leukemia virus progeny having the biologic, antigenic, and biophysical properties of the infecting virus.


Patterns of simian virus 40 deoxyribonucleic acid transcription. II. In transformed cells. The pattern of simian virus 40 (SV40) deoxyribonucleic acid transcription has been examined in 11 SV40-transformed cell lines. In all cases, substantial regions of the minus strand (35-75%) appeared to be transcribed. In the lines tested, these regions included the "early" gene sequences. The SV40-specific ribonucleic acid from at least two of the transformed cell lines represented significantly greater portions of the minus strand than are represented in "early" lytic ribonucleic acid. Small regions of the plus strand appeared to be transcribed in only two of the transformed cell lines.


Oral contraceptives and breast histology.


Replication of simian virus 40 deoxyribonucleic acid: analysis of the one-step growth cycle. The time course of replication of simian virus 40 deoxyribonucleic acid (DNA) was investigated in growing monolayer cultures of subcloned CV1 cells. At multiplicities of infection of 30 to 60 plaque-forming units (PFU)/cell, first progeny DNA molecules (component 1) were detected by 10 hr after infection. During the following 10 to 12 hr, accumulation of virus DNA proceeded at ever increasing rates, albeit in a non-exponential fashion. The rate of synthesis then remained constant, until approximately the 40th hour postinfection, when DNA replication stopped. Under these conditions, the duration of the virus growth cycle was approximately 50 hr. The time needed for the synthesis of one DNA molecule was found to be approximately 15 min. At multiplicities of infection of 1 or less than 1 PFU/cell, the onset of the linear phase of DNA accumulation was delayed, but the final rate of DNA synthesis was the same, independent of the input multiplicity. This was taken as a proof that templates for the synthesis of viral DNA multiply in the cell during the early phase of replication. However, the probability for every replicated DNA molecule to become in turn replicative decreased constantly during that phase. This could be accounted for by assuming a limited number of replication sites in the infected cell.


Denmark's geriatric patients: community care and after-care.


I-cell disease: biochemical studies.

I-cell disease: biochemical studies.


A case of encephalopathic, myelopathic, and peripheral nerve dysfunction in folate deficient megaloblastic anemia.


Mycoplasma species in association with feline viruses.


Studies of the mechanism of induction of infectious murine leukemia virus from AKR mouse embryo cell lines by 5-iododeoxyuridine and 5-bromodeoxyuridine.


The induction of interferon by temperature-sensitive mutants of reovirus, UV-irradiated reovirus, and subviral reovirus particles.


Enucleation of cells made simple and rescue of SV40 by enucleated cells made even simpler.


The effect of dibutyryl cyclic AMP on transformation by oncogenic viruses.


Isolation of leukosis-type virus from pheasant embryo cells: possible presence of viral genes in cells.


Characterization of the SV40 T antigen.

Characterization of the SV40 T antigen.


Effects of aircraft noise on human sleep.


Active transport of calcium ion in sarcoplasmic membranes.


Linear analysis of the dynamics of neural masses.


The mechanism of signal transmission in nerve axons.


Plasticity of biological membranes.

Plasticity of biological membranes.


Isolation and characterization of agglutinin receptor sites. 3. Studies on the interaction with other lectins.


The interaction of a lytic peptide, melittin, with spin-labeled membranes.


Binding and aggregate formation of phospholipid liposomes containing phosphatidic acid with ovalbumin.


Turnover of rat liver plasma membrane phospholipids comparison with microsomal membranes.


Subcellular fractionation of human lymphocytes. Isolation of two plasma membrane fractions and comparison of the protein components of the various lymphocytic organelles.


Herpes zoster of the vulva.

Herpes zoster of the vulva.


Effect of systemic maternal virus infections on the fetus and newborn.