Viral DNA-RNA hybrids in cells infected with simian virus: the simian virus 40 transcriptional intermediates. Brief labeling of cells infected with simian virus 40 with tritiated uridine resulted in the incorporation of part of the label into material that was soluble in 1 M NaCl and sensitive to KOH, with a buoyant density close to that of DNA. The properties of this material suggest that it represents single-stranded nascent molecules of messenger RNA of simian virus 40 hydrogen-bonded over a small portion of their length to viral DNA templates. The name "simian virus 40 transcriptional intermediate molecules" is suggested for these naturally occurring DNA.RNA hybrid molecules. The DNA in the hybrid seems to be in the form of replicative intermediate molecules.


The three-state model: a minimal allosteric description of homotropic and heterotropic effects in the binding of ligands to hemoglobin. The effect of hydrogen ion, chloride, and 2,3-diphosphoglycerate upon the oxygen binding of hemoglobin may be satisfactorily described by an allosteric model employing three affinity states. The allosteric two-state model does not provide even an approximate description except under defined extreme conditions. In particular, the two-state model cannot provide a correct interpretation of recently reported electron spin resonance and nuclear magnetic resonance data.


Cholinergic augmentation of lymphocyte-mediated cytotoxicity. A study of the cholinergic receptor of cytotoxic T lymphocytes. Cholinergic agonists have previously been shown to augment the ability of sensitized lymphocytes to injure cells bearing the sensitizing alleantigens. The cholinergic receptor of the attacking lymphocyte population has been studied with pharmacological manipulation of an in vitro system that quantitates the injury mediated by sensitized attacking cells upon target cells. The data reveal that muscarinic ligands are several orders of magnitude more potent than nicotinic agents in altering cytotoxicity.


Synthesis and properties of Clostridium acidi-urici (Leu2)-ferredoxin: a function of the peptide chain and evidence against the direct role of the aromatic residues in electron transfer. Tyrosyl or other aromatic residues generally occur in two conserved positions in the peptide chain of clostridial-type ferredoxins and have been implicated in the electron transfer function of these iron-sulfur proteins. We have prepared and determined some of the properties of a derivative of Clostridium acidi-urici ferredoxin, [Leu(2)]-ferredoxin, in which a leucyl residue has been substituted for the tyrosyl residue in position 2 from the amino terminus. [Leu(2)]-ferredoxin is fully active as an electron carrier in two biological assays, the phosphoroclastic enzyme system and the ferredoxin-dependent reduction of cytochrome c in the presence of ferredoxin-TPN reductase and TPNH. Quantitative electron paramagnetic resonance experiments indicate that [Leu(2)]-ferredoxin accepts nearly two electrons upon enzymatic reduction by pyruvate-ferredoxin oxidoreductase and an excess of pyruvate. If electron transfer to an iron-sulfur cluster is the rate-limiting step in the assays used, and if the rate of electron transfer through Tyr(30) is not much faster than through Tyr(2), these results indicate that the primary pathway of electron transfer in clostridial-type ferredoxins is not via Tyr or other aromatic amino-acid residues. The syntheses of other ferredoxin derivatives with amino-acid substitutions or deletions in positions 1 and 2 indicate that a large bulky residue, but not necessarily an aromatic residue, is needed in position 2 for the stability of this ferredoxin. The residue in position 2, therefore, appears to act as a hydrophobic shield for an iron-sulfur cluster.


Renal aldosterone receptors: studies with (3H)aldosterone and the anti-mineralocorticoid (3H)spirolactone (SC-26304). In vivo, a spirolactone (SC-26304) inhibited the effects of aldosterone on urinary K(+):Na(+) ratios and the binding of [(3)H]aldosterone to renal cytoplasmic and nuclear receptors. Cytoplasmic binding of [(3)H]aldosterone and [(3)H]spirolactone (SC-26304) was similar in magnitude and involved the same set of sites. Under three sets of conditions-(i) in the intact rat, (ii) in kidney slices, and (iii) in reconstitution studies (mixing prelabeled cytoplasm with either purified renal nuclei or chromatin), [(3)H]spirolactone (SC-26304) did not yield specific nuclear complexes in contrast to the reproducible generation of these complexes with [(3)H]aldosterone. In glycerol density gradients, cytoplasmic [(3)H]aldosterone receptor complexes sedimented at 8.5 S and 4 S in low concentrations of salt and at 4.5 S in high concentrations of salt. Cytoplasmic [(3)H]spirolactone (SC-26304) receptor complexes sedimented at 3 S in low concentrations of salt and 4 S in high concentrations of salt. These results are discussed in terms of an allosteric model of the receptor system.


Electron transfer reactions in biological systems: the reduction of ferricytochrome c by chromous ions. Chromous ion reacts with ferricytochrome c to yield a one-to-one Cr(III)-ferrocytochrome c complex. This material, when hydrolyzed by trypsin and subjected to chromatographic procedures, yielded two fragments containing chromium. The amino-acid compositions and chemical characteristics of each of these fragments indicated that the chromium had crosslinked two segments of polypeptide chain; these were residues 40-53-Cr(III)-residues 61-72 and residues 40-53-Cr(III)-residues 61-73. Examination of a model of the ferricytochrome c molecule indicated that only two residues of the crosslinked peptides were sufficiently close to allow crosslinking to take place. These residues were tyrosine 67 and asparagine 52. Enzymatic hydrolysis of one of those fragments by aminopeptidase M supported this identification. The position of the chromic ion implies what is the path of electron transfer from the chromous ion to the ferric ion in this chemical reduction of cytochrome c, and suggests a possible path of electron transfer in biological oxidation-reduction reactions.


Epstein-Barr virus-associated membrane-reactive antibodies during long term survival Burkitt's lymphoma. Antibodies to Epstein-Barr virus associated cell membrane antigens persisted or slowly decreased during prolonged uncomplicated survival of patients with Burkitt's lymphoma in remission. Serum was sampled from four patients within 6 months before recurrence occurring after the first year and preceded by remission during more than 6 months. Rapid and significant decreases of the antibodies before clinical recognition of the recurrences were found in three patients. The fourth patient had slowly increasing titers before relapse. Immune stimulant treatment during remission, with BCG (Bacille Calmette Guérin vaccine) or BCG + Bordetella pertussis, temporarily increased the antibody levels. Possible mechanisms for the observed titer changes before recurrence are discussed.


Enhancement by carbachol of transmitter release from motor nerve terminals. In the endplates of rat phrenic nerve-diaphragm, application of the acetylcholine-like compound, carbachol, causes a marked increase in transmitter release, as measured electrophysiologically using miniature endplate potential frequency. Washing out of carbachol reverses the increase in frequency. The ability of carbachol to increase transmitter release is greatly enhanced by perfusion of the preparation with Ringer solution containing elevated K(+). At concentrations of carbachol greater than 30 muM, the onset of the postjunctional blocking action of carbachol is too rapid and obscures the increase in miniature potential frequency. The rate of increase in transmitter release is dependent on the concentration of carbachol applied and can be antagonized by d-tubocurarine (10-60 nM) and other blocking compounds. These findings, in contrast to previous reports, indicate that cholinergic nerve endings, like adrenergic nerve endings, respond to applied acetylcholine-like drugs with measurable increases in transmitter output.


Cyclic 3':5'-adenosine monophosphate in Escherichia coli during transient and catabolite repression. Cyclic AMP concentrations were low for an adenylate cyclase-deficient mutant (cya(-)) and abnormally high for a catabolite receptor protein-deficient mutant (crp(-)). A fall in cellular cAMP concentration was always found when cells were subjected to transient repression. No consistent correlations were observed between catabolite repression and cellular cAMP levels.


A molecular model of high density lipoproteins. Based on the analysis of recombined lipidapoprotein complexes by C-13 and P-31 nuclear magnetic resonance spectroscopy and circular dichroism [Assmann, G., Sokoloski, E. A. & Brewer, H. B., Jr. (1974) Proc. Nat. Acad. Sci. USA 71, 549-553; Assmann, G., Highet, R. J., Sokoloski, E. A. & Brewer, H. B., Jr. (1974) Proc. Nat. Acad. Sci. USA 71, in press; Assmann, G. & Brewer, H. B., Jr. (1974) Proc. Nat. Acad. Sci. USA 71, 989-993] and the identification of conformational amphipathic regions in apoproteins, a new model for human high density lipoproteins is proposed. This model is analogous to membrane models proposed by Singer, in that protein "icebergs" are embedded in a "sea" of lipid.


Role of phosphate and other proton-donating anions in respiration-coupled transport of Ca2+ by mitochondria. Measurements of extra oxygen consumption, (45)Ca(2+) uptake, and the osmotic expansion of the matrix compartment show that not all permeant anions are capable of supporting and accompanying the energy-dependent transport of Ca(2+) from the medium into the matrix in respiring rat-liver mitochondria. Phosphate, arsenate, acetate, butyrate, beta-hydroxybutyrate, lactate, and bicarbonate + CO(2) supported Ca(2+) uptake, whereas the permeant anions, nitrate, thiocyanate, chlorate, and perchlorate, did not. The active anions share a common denominator, the potential ability to donate a proton to the mitochondrial matrix; the inactive anions lack this capacity. Phosphate and the other active permeant anions move into the matrix in response to the alkaline-inside electrochemical gradient of protons generated across the mitochondrial membrane by electron transport, thus forming a negative-inside anion gradient. It is postulated that the latter gradient is the immediate "pulling" force for the influx of Ca(2+) on the electrogenic Ca(2+) carrier in respiring mitochondria under intracellular conditions. Since mitochondria in the cell are normally exposed to an excess of phosphate (and the bicarbonate-CO(2) system), particularly in state 4, inward transport of these proton-yielding anions probably precedes and is necessary for inward transport of Ca(2+) and other cations under biological conditions. These observations indicate that a negative-inside gradient of phosphate generated by electron transport is a common step and provides the immediate motive power not only for (a) the inward transport of dicarboxylates and tricarboxylates and (b) the energy-dependent exchange of external ADP(3-) for internal ATP(4-) during oxidative phosphorylation, as has already been established, but also for (c) the inward transport of Ca(2+), K(+), and other cations.


Renal arteriovenous fistula secondary to metastatic tumor.


Wilms' tumor. Treatment in an adult.

Wilms' tumor. Treatment in an adult.


Effect of prolonged lithium ingestion on glucagon and parathyroid hormone responses in rats.


Endocrine laboratory assays in obstetrics and gynecology.


Antifungal properties of polymyxin B and its potentiation of tetracycline as an antifungal agent. High concentrations of polymyxin B inhibited the growth of Candida albicans and Saccharomyces cerevisiae. When these yeasts were incubated with concentrations of polymyxin B too low to affect growth, and were then exposed to tetracycline, protein synthesis was inhibited and at least 99% of the organisms were killed. Neither inhibition of protein synthesis nor cell death occurred in cultures treated with high concentrations of tetracycline alone. We conclude that polymyxin B at high concentrations affects the cell membrane of yeasts, which results in inhibition of growth. At low concentrations, it increases the permeability of the yeast cell membrane to tetracycline, which then inhibits protein synthesis and leads to cell death.


Location of the T4 gene 32 protein-binding site on polyoma virus DNA.


Interaction of N-methyl phenazinium radical cation with single-stranded polyriboadenylic acid.


Localization of major group-specific polypeptides in the avian RNA tumor viruses.


Coupling of polyoma DNA and RNA synthesis.


Flavin binding by bacterial luciferase: affinity chromatography of bacterial luciferase.


Translation of mengovirus RNA in Ehrlich ascites cell extracts.


The effect of altered sterol composition on cytochrome oxidase and S-adenosylmethionine: delta 24 sterol methyltransferase enzymes of yeast mitochondria.


A new eukaryotic RNA polymerase factor: a factor from chicken myeloblastosis cells which stimulates transcription of denatured DNA.


Isolation and terminal sequence determination of the major rat brain myelin proteolipid P7 apoprotein.


Effect of NH+4 and K+ on the activity of the ribosomal subunits in the EF-G- and EF-T-dependent GTR hydrolysis.


Interaction of lysolecithin micelles and lecithin vesicles with apolipoprotein Gln I from serum high density lipoproteins.


ALA synthetase induction. Inhibitory effect exerted by administration of dibutyryl adenosine-3',5'-cyclic monophosphate, theophylline and caffeine.


Comparative study on induction of hepatic delta-aminolevulinate synthetase by different drugs.


Are both fractions of Rhodopseudomonas spheroides Y ALA synthetase isoenzymes?


Protohaem synthesis by the yeast Saccharomyces cerevisiae during respiratory adaptation. Relationships with glycogen metabolism.


Vitamin D3 metabolites. I. Synthesis of 25-hydroxycholesterol.


Vitamin D3 metabolites. II. Further syntheses of 25-hydroxycholesterol.


The cytoproct of Paramecium caudatum: structure and function, microtubules, and fate of food vacuole membranes.


Vitamin D3 metabolites. 3. Synthesis and X-ray analysis of 1 alpha,25-dihydroxycholesterol.


Cell hybridization and cancer.

Cell hybridization and cancer.


Molecular pathology of the cancer cell.

Molecular pathology of the cancer cell.


Tumour-associated hormonal products.

Tumour-associated hormonal products.


Effects of tumour viruses on cell growth.


Characteristics of Sendai virus receptors in a model membrane.


Hormones and neoplasia.

Hormones and neoplasia.


Replication of polyoma DNA in isolated nuclei. 3. The nucleotide sequence at the RNA-DNA junction of nascent strands.


Letter: Evidence for non-repetitive subunits in the genome of Rous sarcoma virus.


Logical analysis of the mechanism of protein folding II. The nucleation process.


A re-examination of the renal blastema graft model for Wilms tumor production.


Editorial: Vaccine against cytomegalovirus.


Spread of an avian adenovirus (CELO virus) to uninoculated fowls.


The survival of foot-and-mouth disease virus in African buffalo with non-transference of infection to domestic cattle.


Errata: Demonstration of platelet fibrinogen secretion via the surface connecting system.


Influence of human ceruloplasmin on the aggregation of human platelets by adenosine diphosphate.


Studies on the pathogenesis of iodide myxedema.


The mechanism of activation of hormone-sensitive lipase.


Action of cholera toxin to stimulate adenyl cyclase.


Extracellular deoxyribonuclease production by anaerobic bacteria. The production of extracellular deoxyribonuclease was examined with anaerobic organisms isolated from clinical specimens. Nuclease activity was extraordinarily common. All strains of Fusobacterium, including eight species, as well as Bacteroides fragilis and B. melaninogenicus, displayed enzyme activity. Whereas the gram-positive bacteria were generally less productive, all strains of Clostridium perfringens, Peptostreptococcus intermedius, and P. anaerobius specifically produced deoxyribonuclease. The test is taxonomically valuable, particularly in the characterization of gram-positive cocci, since a deoxyribonuclease-producing coccus indicates P. intermedius or P. anaerobius. Additionally, possession of the enzyme may prove to be a useful correlate of the potential pathogenicity of anaerobes.


Method for isolation and selection of temperature-sensitive mutants of herpes simplex virus. A method for induction and selection of temperature-sensitive mutants of herpes simplex virus is presented. After the infected cells were treated with 5-bromodeoxyuridine, the virus was extracted by repeated freezing and thawing and cloned in microcultures which were then incubated at permissive temperature until viral plaques appeared. The microcultures were then replicated at nonpermissive temperature. Clones not forming plaques in these latter were further purified and examined for temperature-sensitive characteristics. Viral clones mutated in plaque-forming ability or in yield were obtained and preliminarily characterized.


Effect of culture medium and carbon dioxide concentration on growth of anaerobic bacteria commonly encountered in clinical specimens. Representative strains of anaerobic bacteria from human infections were used to evaluate broth media, gas mixtures, and inocula for use in developing a procedure for performing minimal inhibitory concentration antimicrobic susceptibility tests. Nine commercially available media, including two that were chemically defined, were tested. Tests were performed in atmospheres with carbon dioxide concentrations between 2.5 and 10% and also in the GasPak system (BBL) that had a disposable hydrogen-carbon dioxide generator. Growth curves on each organism grown in schaedler broth and a 5% carbon dioxide atmosphere were used to determine growth characteristics, equate time of the particular growth phases to turbidity readings, and determine the numbers of viable organisms present in the culture. Schaedler broth proved to be most advantageous in combination with an atmosphere of 5% carbon dioxide, 10% hydrogen, and 85% nitrogen. The growth curve studies yielded valuable data on the rapidity and quantity of growth under these conditions. We believe these data have provided information which can be used as the basis for developing a standardized procedure for antimicrobic susceptibility testing for anaerobic bacteria.


Apparatus for conditioning unlimited quantities of finished waters for enteric virus detection. An apparatus has been developed, constructed, and tested for conditioning unlimited quantities of finished waters for enteric virus detection.


Metabolic adaptations in skeletal muscle of streptozotocin-diabetic rats following exercise training.


Incorporation of 131-I-labeled androgen-receptor into nuclei of rat prostates.


The influence of cyclic GMP on polypeptide synthesis in a cell-free system derived from wheat embryos.


The conformation of oxytocin in dimethylsulfoxide as revealed by carbon-13 spin-lattice relaxation times.


An apolipoprotein preferentially enriched in cholesteryl ester-rich very low density lipoproteins.


Conversion of diglyceride to triglyceride by rat liver microsomes: a requirement for the 105,000 x g supernatant.


Cobalt cytochrome C: preparation of characterization.


Action of Solanum malacoxylon on calcium metabolism in the rat.


Role of lecithin in D-beta-hydroxybutyrate dehydrogenase function.


Selective chemical modification of arginine residues in mitochondrial malate dehydrogenase.


In vivo regulation of hepatic protein kinase by adenosine 3',5'-monophosphate mediated glucagon stimulation.


Synthesis of glucosylceramide by purified calf spleen beta-glucosidase.


Soluble & membrane-bound superoxide dismutases in a blue-green algae (Spirulina)and spinach.


Discharge patterns of cat raphe neurons during sleep and waking.


Developmental variations of choline acetyl-transferase, dopamine-beta-hydroxylase and monoamineoxidase in chicken embryo and chicken sympathetic ganglia.


Connectivity changes of Ia afferents on axotomized motoneurons.


Two cAMP-activated protein kinases from bovine thyroid.


Streptomycin resistance and ribosomal proteins: amino acid substitutions in the str protein of one streptomycin-resistant mutant of Escherichia coli K12 after mutagenesis with ethyl methanesulfonate.


The synthesis of diastereomeric epoxy-(beta-D-glucopyranosyl)ethanes and 1,2-epoxy-3-(beta-D-glucopyranosyl)propanes, as irreversible inhibitors of beta-D-glucosidase.


Identification of iduronic acid as a constituent of the "type-specific" polysaccharide of Clostridium perfringens Hobbs 10.


The ultrastructure of the accessory sex organs of the male rat. VI. The effect of cyproterone acetate on the dorsal lobe and the coagulating gland.


The development and fine structure of the ultimobranchial glands in larval Rana temporaria L.


Developmental genetics of teleosts: a biochemical analysis of lake chubsucker ontogeny.


The content of pyridine dinucleotides in the mouse embryo before implantation.


Effect of phenobarbital administration on the subcellular distribution of vitamin D3-3H in rat liver.


Effects of calcitonin in the concentration of cyclic adenosine 3',5'-monophosphate in rat kidney in vivo and in vitro.


Embryonic chick intestine in organ culture: interaction of adenylate cyclase system and vitamin D3-mediated calcium absorptive mechanism.


Purified somatotrophs from rat adenohypophysis: response to secretagogues.


Thyrotropin-releasing hormone stimulation and somatostatin inhibition of growth hormone secretion from perfused rat adenohypophyses.


Observations on the effects of chromate on the thyroid.


Secretion of ACTH by isolated anterior pituitary cells: kinetics of stimulation of corticotropin-releasing factor and of inhibition by corticosterone.


Mineralocorticoid receptors in salt-susceptible and salt-resistant rats.


Lead-induced inclusion bodies: composition and probable role in lead metabolism. Lead-induced inclusion bodies in renal tubular cells of rats have been studied in vitro after isolation by differential centrifugation. The inclusion bodies are insoluble in physiological media but may be dissolved in denaturants like 6M urea and sodium deoxycholate. They contain about 40-50 mug of lead/mg protein, but only about 10% of this is tightly bound. They also contain calcium, iron, zinc, copper, and cadmium. The protein is rich in glutamic and aspartic acids, glycine and cystine. When dissolved in 6M urea, the protein migrates as a single band on acrylamide gel electrophoresis and has a molecular weight of 27,500. It is suggested that the inclusion bodies function as an intracellular depot of nondiffusible lead. Further studies have been directed toward finding a free, unaggregated lead-containing protein fraction. Nuclear proteins from kidneys of lead-toxic rats were separated into NaCl-, Tris-, and NaOH-soluble fractions and an insoluble acidic fraction. A quantitatively small lead-containing protein was found in the 0.14M NaCl fraction. Amino acid composition, electrophoretic mobility, molecular weight, and ability to bind lead are similar to those of insoluble inclusion body protein. The possible role of this soluble lead-binding protein in the formation of nuclear inclusion bodies is at present time not certain. These studies do suggest, however, that protein-bound lead in renal tubular cells may be partitioned between insoluble and nondiffusible morphologically discrete inclusion bodies and a soluble, extractable fraction which is presumably diffusable.


Lead aggravates viral disease and represses the antiviral activity of interferon inducers. Lead acetate was administered continuously in the drinking water to CD-1 male mice beginning at 4 weeks of age. An LD(10-20) of the lytic viruses or 300 plaque-forming units of RLV was inoculated intrapertioneally at 6 weeks of age. Lead increased the response of the mice to all classes of viruses against which it was tested: an RNA picornavirus-encephalomyocarditis (EMCV), a DNA herpesvirus-pseudoribies, an RNA leukemia-virus-Rauscher leukemia (RLV), an RNA arbovirus B-St. Louis encephalitis, and an RNA arbovirus A-western encephalitis. Most studies were performed between lead and EMCV. Increases in EMCV mortality in lead treated mice over controls ranged from 2x at a lead level of 0.004M to 7x (100% mortality) at a 0.1M lead level. Splenomegaly with spleens 800 to 1100 mg in weight containing high titers of RLV occurred in lead (0.03M)-treated mice 3 and 6 weeks after RLV inoculation; spleens or RLV controls were normal in weight (200 mg) and were free of virus. Lead did not reduce the protective effect of mouse interferon (IF) against the lethal action of EMCV, but it did repress the EMCV antiviral effect of poly I/poly C (PIC) and of Newcastle disease virus (NDV) against EMCV mortality. These data indicate several new facts concerning adverse effects lead may have on an animal: (1) lead aggravates viral disease, most likely in part, through reduced IF synthesis; (2) lead represses the anti-EMCV protective effects of both PIC and of NDV, which, in other reports, were shown to induce IF in radioresistant macrophages (PIC) or in radiosensitive lymphocytes (NDV); (3) lead may then be said to repress IF induction in two kinds of cells; (4) however, lead does not inhibit IF action.


Demonstration of a reaggregation inhibitor in sea urchin embryos.


Retraction fibers and leucocyte chemotaxis.


Red cell lead, whole blood lead, and red cell enzymes. Simultaneous assay of blood lead (Pb-B) and red cell lead (Pb-Rbc) in 123 samples from 104 urban and suburban students, ages 10-18, shows the ratio of concentration (Pb-Rbc)/(Pb-B) to increase as the hematocrit decreases. On direct assay in 40 samples, plasma lead (Pb-P) was fixed in a narrow range. In 28 students with Pb-Rbc >40 mug/100 ml, the mean red cell 2,3-diphosphoglycerate (2,3-DPG) was 6.05+/-0.28 (+/-S.E.), significantly higher (P<.025) than the 5.25+/-0.18 of 51 students with Pb-Rbc<40 mug/100 ml, although hemoglobin values were comparable (13.83+/-0.31 versus 13.55+/-0.20). Analysis of the individual population groups showed this correlation of Pb-Rbc with 2,3-DPG to be primarily related to the intercorrelation of each parameter with hemoglobin.Rbc membrane Na/K ATPase, as per cent of total membrane ATPase, had a median value of 60% in 48 subjects. Na/K ATPase below 60% was found in 10 (77%) of the 13 students with Pb-Rbc>/=40 mug/100 ml, but in only 14 of the 35 with Pb-Rbc<40 mug/100 ml (chi(2)=5.1, df=1, P<0.05). Correlation of significant enzyme changes with Pb-Rbc, but not with Pb-B in the normal urban range of Pb-B<35 mug/100 ml suggests Pb-Rbc, increased in anemia, to be a critical factor in the hematotoxicity of lead.


Effect of proteolytic inhibitors on growth and surface architecture of normal and transformed cells.


Uncoupler-stimulated adenosine triphosphatase activity. Deficiency in intact mitochondria from Morris hepatomas and ascites tumor cells.


Sugar transport in chick embryo fibroblasts. II. Alterations in transport following transformation by a temperature-sensitive mutant of the Rous sarcoma virus.


The isolation and partial characterization of two novel sphingolipids from Neurospora crassa: di(inositolphosphoryl)ceramide and ((gal)3glu)ceramide.


Inositol phosphorylceramide, a novel substance and the chief member of a major group of yeast sphingolipids containing a single inositol phosphate.


Nucleoside diphosphokinase of Salmonella typhimurium.


Thermodynamic parameters for the hydrolysis of inorganic pyrophosphate at pH 7.4 as a function of (Mg2+), (K+), and ionic strength determined from equilibrium studies of the reaction.


Inhibition of macromolecular synthesis in Escherichia coli by protease inhibitors. Specific reversal by glutathione of the effects of chloromethyl ketones.


Regulation of protein synthesis during density-dependent growth inhibition of BHK21-13 cells.


Isolation and relationship of human hexosaminidases.


The equilibria and reaction rates of nicotinamide-adenine dinucleotide and its related compounds with cyanide ion in the presence of polyelectrolytes.


Differential effects of adenylyl imidodiphosphate on adenosine triphosphate synthesis and the partial reactions of oxidative phosphorylation.


Studies with cyclic adenosine monophosphate receptor and stimulation of in vitro transcription of the Gal operon.


Stimulation and desensitization of macrophage adenylate cyclase by prostaglandins and catecholamines.


The enhancement of macrophage adenylate cyclase by products of activated lymphocytes.


Casein kinase from the Golgi apparatus of lactating mammary gland.


Formation of a novel nucleotide by fat cell preparations containing adenylate cyclase.


The determination of low density lipoproteins by micro-nephelometry: a practical assessment.


A histochemical characterization of malignant tumour mucins as a possible aid in the identification of metastatic deposits.


Cystosarcoma phylloides in a twelve-year-old girl.


Studies on growth homone secretion. VI. Effects of dibutyryl cyclic AMP, prostaglandin E1, and indomethacin on growth and hormone secretion by rat pituitary tumor cells in culture.


Cyclic AMP regulation of prostaglandin biosynthesis in fat cells.


The effect of L-tryptophane upon the elaboration and extinction of a conditioned avoidance response and upon the behaviour of rats.


Cyclic AMP-dependent protein kinase activity in a cell membrane-enriched subcellular fraction of pig myocardium.


Binding of calcium to a cell membrane-enriched preparation from pig myocardium: increase in calcium affinity upon membrane protein phosphorylation enhanced by a membrane-bound cyclic AMP-dependent protein kinase.


Subcellular localisation and properties of contractile protein ATPases of blood platelets.


Cyclic AMP and platelet aggregation.

Cyclic AMP and platelet aggregation.


The release reaction.

The release reaction.


Platelet aggregation in the rat--relation to human thrombopathias.


Enzymes of purine metabolism in platelets: phosphoribosylpyrophosphate synthetase and purine phosphoribosyltransferases.


Hypoxanthine-guanine phosphoribosyltransferase deficiency: altered kinetic properties of a specific mutant form of the enzyme.


Mutant phosphoribosylpyrophosphate synthetase in two gouty siblings with excessive purine production.


An unusual case of glycogen storage disease.


Human glltamine phosphoribosylpyrophosphate (PP-ribose-P) amidotransferase: kinetic, regulation and configurational changes.


Increased PP-ribose-P synthetase activity: a genetic abnormality leading to excessive purine production and gout.


Human phosphoribosylpyrophosphate (PP-ribose-P) synthetase: properties and regulation.


Regulatory aspects of the synthesis of 5-phosphoribosyl-1-pyrophosphate in human red blood cells.


Xanthine oxidase and aldehyde oxidase in purine and purine analogue metabolism.


Familial association of metabolic myopathy, lactic acidosis and sideroblastic anemia.


Hormonal control of skeletal and mineral homeostasis.


Catalytic properties of three lactate dehydrogenases from potato tubers (Solanum tuberosum).


Fat metabolism in higher plants. Recent studies on plant alpha-oxidation systems.


Effects of visual pigments on the organization of phospholipid multibilayer model membranes--a spin probe study.


Regulatory sulfhydryl groups, conformational change, and allosteric inhibition in rabbit muscle fructose diphosphatase.


The effect of sulisoxazole (Gantrisin) and albumin on bilirubin conjugation in cultures of a clonal cell line with liver-like functions.


Changes in apolipoproteins and properties of rabbit very low density lipoproteins on induction of cholesteremia.


The mode of action of metronidazole in Trichomonas vaginalis and other micro-organisms.


Antibodies to the unfolded form of a helix-rich region in staphylococcal nuclease.


Two pictures of a lipid bilayer. A comparison between deuterium label and spin-label experiments.


Tritium-hydrogen exchange of the cyclic peptide polymyxin B-1.


Magnetic resonance studies of the geometry of bound nicotinamide adenine dinucleotide and isobutyramide on spin-labeled alcohol dehydrogenase.


Specificity of chemical modification of ribonucleic acid transport by liver carcinogens in the rat.


Enzymatic synthesis of deoxyribonucleic acids with repeating sequences. A new repeating trinucleotide deoxyribonucleic acid, d(T-C-C)n-d(G-G-A)n.


Binding of lanthanides and of divalent metal ions to porcine trypsin.


Protective effect of interferon on mice experimentally infected with Japanese encephalitis virus.


Improving psychiatric drug therapy. A matter of dosage and choice.


Inhibition of hepatoma-immune lymph-node cell cytotoxicity by tumour-bearer serum, and solubilized hepatoma antigen.


Inheritance of susceptibility to Friend mouse leukemia virus. X. Separate genetic control of two viruses in Friend virus preparation.


Specific neutralizing antibodies in Epstein-Barr virus associated diseases.


Influence of age and 3-methylcholanthrene on azo-dye carcinogenesis and metabolism of p-dimethylaminoazobenzene in rat liver.


The effects of long-term feeding of DDT to BALB-c mice.


Increase in viral antigen in individual polyoma-virus-infected mouse kidney cells as studied by quantiative immunofluorescence.


A rapid destructive effect of the Schmidt-Ruppin strain of Rous sarcoma virus on transformed rat cell lines.


Extraction and analysis of carotenoids in fresh plant materials.


Genetic control of MuLV-gs expression in crosses between high and low leukemia incidence strains.


Cherubism.

Cherubism.


Failure to detect, in human sera, antibodies cross-reactive with group-specific antigens of murine leukemia virus.


Rapid screening method for analysis of chlorinated pesticide and polychlorinated biphenyl residues in fish.


Rapid screening method for examining corn and corn-derived products for possible aflatoxin contamination.


A rapid dialysis cell in the zirconyl gel assay for carcinoembryonic antigen in human serum.


Chemodectomas in dogs: epidemiologic comparisons with man.


Responses of isolator-derived and conventional chickens to Marek's disease herpesvirus and avian leukosis virus.


Prevalence of endogenous type-C virus in normal hamster tissues and hamster tumors induced by chemical carcinogens, simian virus 40, and polyoma virus.


Effect of aminoacetonitrile on the metabolism of diethylnitrosamine during liver carcinogenesis.


Transmission of the mammary tumor virus by the GR mouse strain. II. Genetic studies.


Potential carcinogens. I. Carcinogenicity of some plant extracts and their tannin-containing fractions in rats.


Immunotherapy of cancer: regression of established intradermal tumors after intralesional injection of mycobacterial cell walls attached to oil droplets.


BALB-cfR3: a new high mammary-tumor and high leukemia mouse strain.


Protection against guinea pig hepatomas by pretreatment with subcelluar fractions of Mycobacterium bovis (BCG).


Hepatocarcinogenic material in urine specimens from humans consuming aflatoxin.


A one cell-cycle delay for tumor cytostasis in vitro: effects of dibutyryl cyclic AMP or embryonic extracts on synchronized cultures of a murine tumor.


Effect of soluble and membrane proteins upon diethyl ether extraction of aqueous phospholipid dispersions.


Bis-(monoacylglyceryl)phosphate and acyl phosphatidylglycerol isolated from human livers of lipidosis induced by 4,4'-diethylaminoethoxyhexesterol.


Phospholipids of two strains of dermatophyte, Arthroderma uncinatum.


Biological characters of Staphylococcus aureus isolated from diseased chickens.


Axonal filamentous spheroids associated with cardiomyopathy with "targetoid fibers". I. Clinical, histologic, and electron microscopic studies.


Malignant tumors of the trachea. A new outlook.


Diabetes and Addison's disease.

Diabetes and Addison's disease.


Flunitrazepam (Ro 5-4200) and sleep cycle in insomniac patients.


Cytochemical observations on the midpiece of the spermatozoa of the gastropod Biomphalaria glabrata.


A case of Raynaud's syndrome of peculiar origin.


Is the inhibition of glutamic-pyruvic transaminase by phenylalanine one of the causes of hypoglucosemia in phenylketonurics?


Latest developments in the treatment of amebiasis.


Adrenocortical responses of the Apollo 17 crew members.


In vitro antiviral activity and preliminary clinical trials of a new adamantane compound. A compound, 1'-methyl spiro (adamantane-2,3'-pyrrolidine) maleate, chemically related to the antiviral drug amantadine, was tested for activity in vitro against a number of human respiratory viruses. By a variety of techniques, it was shown to be active against a wide range of human and animal influenza A viruses. The effect was, however, variable and ranged from high activity against two 1957 Asian strains to no observable activity against a 1971 strain. Like amantadine, the drug did not inhibit the growth of influenza B viruses. It was also inactive against a number of paramyxoviruses. Unlike amantadine, the drug did inhibit rhinoviruses, but to a lesser extent than myxoviruses. The coronavirus 229E was also sensitive to the action of the drug in vitro. Although an earlier trial in volunteers showed that, when given orally from 2 days before until 5 days after virus challenge, the drug was protective against infection with influenza A/Hong Kong/68 virus, a similar trial in volunteers challenged with rhinoviruses 2 and 9 revealed no useful activity against rhinoviruses in man.


Antiviral activity of cytarabine in herpesvirus-infected rats. Intranasal inoculation of herpesvirus (approximately 1.8 mean lethal doses [LD(50)] in 0.1 ml) into 105- to 115-g rats produces paralytic disease in 4 to 5 days and 80 to 100% mortality in 8 to 12 days. Cytarabine (ara-C) (40 to 320 mg/kg), administered subcutaneously to inoculated rats, delays the onset of paralysis and protects the animals from death. Drug treatments were given twice daily for 5 days. Beneficial drug effects were observed even when initiation of therapy was delayed for 3 days after virus inoculation. A dose-response relationship existed when therapy was initiated at 4 h after virus inoculation. However, when therapy was delayed for 3 days, it appeared that the highest drug level (320 mg/kg twice daily) was somewhat less effective than the lower doses (160 and 80 mg/kg twice daily). Virus could be detected in homogenates of brain beginning 3 days after inoculation, and the titer increased through 7 days. Ara-C treatment, initiated 4 h after inoculation, caused a delay in the appearance of virus, and a reduction in the titer in the brain homogenates. No virus was detected in blood serum, or in homogenates prepared from lung, kidney, thymus, or spleen of infected rats. The virus titration studies are in agreement with the illness and mortality produced by herpesvirus infection.


Regulation of the diurnal rhythm of rat liver beta-hydroxy-beta-methylglutaryl coenzmye A reductase activity by insulin, glucagon, cyclic AMP and hydrocortisone.


Thermodynamic relationships between the oxidation-reduction reactions and the ATP synthesis in suspensions of isolated pigeon heart mitochondria.


Formation of the yeast mitochondrial membrane. 3. Accumulation of mitochondrial proteins synthesized in both the cytoplasm and mitochondria in yeast undergoing glucose depression.


The use of 13C spin lattice relaxation times to study the interaction of alpha-methyl-D-glucopyranoside with concanavalin A.


Factors affecting the formation of phytol and its incorporation into chlorophyll by homogenates of the leaves of the french bean Phaseolus vulgaris.


Stimulation of the incorporation of 32Pi and myo-(2-3H)inositol into the phosphoinositides in polymorphonuclear leukocytes during phagocytosis.


Inhibition of nucleic acid synthesis in Ehrlich tumor cells by periodate-oxidized adenosine and adenylic acid.


Heme-heme interaction in cytochrome c oxidase: the cooperativity of the hemes of cytochrome c oxidase as evidenced in the reaction with CO.


Ferrochelatase activity in wild-type and mutant strains of Spirillum itersonii. Solubilization with chaotropic reagents.


New cyclopentenyl fatty acids in Flacourtiaceae. Straight-chain fatty acids and cyclic fatty acids in lipids during maturation of the seeds.


Adenosine diphosphate as a regulatory ligand in beef heart cytosol nucleoside diphosphokinase.


Molecular characterization of proteins in detergent solutions.


Alterations of the aryl hydrocarbon hydroxylase system during riboflavin depletion and repletion.


Conformation of angiotensin II in aqueous solution. Titration of several peptide analogs and homologs.


Effect of magnesium adenosine 5'-triphosphate on the accessibility of the iron of clostridial azoferredoxin, a component of nitrogenase.


A convenient micro method for the quantitation of closed circular deoxyribonucleic acid.


Partial characterization of the sexual agglutination factor from Hansenula wingei Y-2340 type 5 cells.


Bone turnover-sex hormone-parathyroid hormone interrelationships in postmenopausal osteoporosis.


The mechanism of SV40 DNA replication.

The mechanism of SV40 DNA replication.


The temporal order of replication of some DNA cistrons.


Replication of mitochondrial DNA: replicative forms of molecules from rat tissues and evidence for discontinuous replication.


Genetic organization and regulation of the Drosophila Y chromosome.


Recent DNA fiber diffraction studies and their implications for DNA recognition.


Some properties of RNA:protein complexes from the nucleus of eukaryotic cells.


Cleavage of mouse DNA by a restriction enzyme as a clue to the arrangement of genes.


A comparison of DNA binding proteins from normal and virus-transformed mouse cells.


Intracerebral transplantation of human rhabdomyosarcoma cells into fetal and newborn kittens.


Mammary tumor virus (MTV) antigens in normal and mammary tumor-bearing mice.


Differences in cytochalasin B inhibition of 3-O-methylglucose uptake between BALB-3T3 cells and a murine sarcoma virus transformed clone.


A study of herpes simplex type 2 antibody status in groups of patients with cervical neoplasia in Czechoslovakia.


Influence of interferon on virus particle formation in different oncornavirus carrier cell lines.


Serum effects on cell-mediated destruction of Rous sarcomas.


The humoral antibody response to a primary viral neoplasm (MSV) through its entire course in BALB-c mice.


The histogenesis of tumours induced in golden hamsters by murine sarcoma virus-Harvey (MSV-H).


Carcinogenicity of nitrosamines and methanesulphonate esters given intraperitoneally, in RF mice.


Antibody to Epstein-Barr virus in man in Australia and New Guinea.


Cyclic-AMP in ocular tissues of the rabbit, monkey, and human.


Carbon-13 nuclear relaxation measurements in nicotinamide adenine dinucleotide and adenosine monophosphate.


Kinetic studies of the reduction of ferricytochrome c by Fe(EDTA)2-.


Effects of a prolonged 3-hour sleep-wake cycle on sleep stages, plasma cortisol, growth hormone and body temperature in man.


A rapid assay for 25-OH-vitamin D3 without preparative chromatography.


Oxytocin receptors in human uterus.

Oxytocin receptors in human uterus.


Recovery of hypothalamic-pituitary-adrenal function, growth hormone responsiveness and sleep EEG pattern in a patient following removal of an adrenal cortical adenoma.


A study of thyrotropin deficiencies by the combination of two tests of TSH function.


Hypothalamic-pituitary function in the olfacto-genital syndrome.


Multiple hormones in a bronchial tumor.

Multiple hormones in a bronchial tumor.


Glandular end organ deficiency associated with secretion of biologically inactive pituitary peptides.


Prostaglandin E (PGE) control of cell proliferation in vitro: characteristics of HT-29.


Editorial: The value of scanning in hepatic cirrhosis.


The agent of trachoma. Recent studies of the biology, biochemistry and immunology of a prokaryotic obligate parasite of eukaryocytes.


In vitro and in vivo phosphorylation of myelin basic protein by cerebral protein kinase.


Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.


Induced redistribution of membrane particles, anionic sites and con A receptors in Entamoeba histolytica.


Urinary excretion of carnitine in progressive muscular dystrophy.


Production of O2- in photolyzed water demonstrated through the use of superoxide dismutase.


Biologic exposure to dental materials.

Biologic exposure to dental materials.


Protein binding of 3',5'-adenosine cyclic monophosphate acid.


Association of cowpea mosaic virus-induced double-stranded RNA with a cytopathological structure in infected cells.


Heterogeneity of the inner mitochondrial membrane through the unequal distribution of phospholipid unsaturation.


A nonselective analysis of SV40 transformation of mouse 3T3 cells.


Characteristics of murine C-type viruses. II. The behavior of viruses resident in various cell lines and their hybrids on BALB-3T3 and mouse embryo fibroblast cultures.


Isolation of defective viruses from SV40-transformed human and hamster cells.


Maturation of vesicular stomatitis virus: electron microscopy of surface replicas of infected cells.


The involvement of NADH-cytochrome b5 reductase and cytochrome b5 complex in microsomal NADH-cytochrome c reductase activity. Resolution of the complex by triton X-100.


The involvement of NADH-cytochrome b5 reductase and cytochrome b5 complex in microsomal NADH-cytochrome c reductase activity. Changes in NADH-cytochrome c reductase activity following phenobarbital treatment.


Inhibition of influenza and parainfluenza virus replication in tissue culture by 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA).


Labyrinthine morphology and temperature in cryosurgery (guinea pig).


Isoleucyl transfer ribonucleic acid synthetase from Escherichia coli. Effect of limited cleavage by trypsin on activity and structure.


Fluorescence energy transfer between the thiamine diphosphate and flavine adenine dinucleotide binding sites on the pyruvate dehydrogenase multienzyme complex.


Fluorescent affinity labeling of initiation site on ribonucleic acid polymerase of Escherichia coli.


Conformational transitions of cyclic adenosine monophosphate receptor protein of Escherichia coli. A temperature-jump study.


Conformational transitions of cyclic adenosine monophosphate receptor protein of Escherichia coli. A fluorescent probe study.


Interaction of apolipoprotein-alamine and apolipoprotein-glutamine-I with phosphatidylcholine. Effect on solute quenching of intrinsic fluorescence.


A new method of assay for polynucleotide ligase.


Chemical synthesis of ppGpp.

Chemical synthesis of ppGpp.


Serum lipoprotein apoproteins as major protein constituents of the human erythrocyte membrane.


Magnetic studies on the changes in the iron environment in Chromatium ferricytochrome c'.


Effect of neuraminidase on the synthesis of collagen and other proteins.


A method for the preparation of desialylated human chorionic gonadotropin and its sub-units.


Aberrant carotid artery simulating a tumor of the middle ear.


Effects of antibiotics on intracellular symbiotes in the pea aphid, Acyrthosiphon pisum.


A comparative ultrastructural study of the pars tuberalis of various mammals, the chicken and the newt.


The cell-types in the adenohypophysis of the Indian catfish Heteropneustes fossilis (Bloch).


Fine structure of frog lime sacs.

Fine structure of frog lime sacs.


Comparative studies on porcine and human high density lipoproteins.


Intraependymal cerebrospinal fluid contacting neurons and axon terminals on the external surface in the filum terminale of the carp (Cyprinus carpio).


Characterization and quantification of a crustacean lipovitellin.


Some enzymic reactions of adenine derivatives in the tail muscle of the lobster, Homarus americanus.


The fine structure of the digital pads of rhacophorid tree frogs.


Fish liver fructose 1,6-diphosphatase. II. "Neutral" and "alkaline" forms.


DNCB reactivity in patients with skin carcinoma.


The amino acid sequence of ostrich (Struthio camelus) cytochrome c.


Modulation of mitochondrial adenylate kinase by citric-acid-cycle intermediates.


The effect of lanthanide ions on the conformation of adenine mononucleotides and dinucleotides.


Characterization of the single-strand-specific nuclease S1 activity on double-stranded supercoiled polyoma DNA.


Structural studies on phenylalanine transfer ribonucleic acid from yeast with the spectroscopic label formycin.


Intracellular cyclic AMP level and intestinal smooth muscle relaxation.


Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. The "killer character" of Saccharomyces cerevisiae is a nonchromosomal genetic element which imparts to cells carrying it (a) the ability to kill cells which lack it, by secreting the soluble macromolecular killer substance and (b) the ability to resist the killing effects of the killer substance.-Mutants have been isolated from S. cerevisiae carrying the "killer character". Mutants were found in two nuclear genes (mak1 and mak2) involved in maintenance of the genetic element of the killer character. Mutants in three other nuclear genes could maintain the cytoplasmic genetic element normally; two of these were able to express resistance to killing, but not the ability to kill (kex1 and kex2), while the third could express the killing function, but was sensitive to killing and is thus a "suicide" strain (rex1). These five nuclear genes were all distinct.-Several mutants were detected which showed non-mendelian segregation indicating mutation of the "cytoplasmic" genetic element. Some such mutants had normal resistance to killing but were either unable to kill at all or showed marked reduction in the ability to kill. Others had lost both killing ability and resistance. None were suppressive.-Two nonkilling mutants, both of which showed cytoplasmic inheritance, were mated. A large, but variable, proportion of diploid colonies, plated after several generations of growth, showed normal killing which has then stably inherited on further subcloning.


Interactions between peripheral and hypothalamic carbachol stimulation of drinking in the rat.


Phosphatases within the cartilage of the mandibular condyle of the mouse.


Proceedings: The effect of cyclic AMP and cyclic GMP on the histology of the neonatal rat hepatocytes.


Proceedings: Evidence that large granular vesicles are not involved in transmitter release from adrenergic nerves.


Proceedings: Adrenergic and cholinergic innervation of arteriovenous anastomoses: the effect of 6-hydroxydopamine.


The rapid isolation of plant virus RNAs using sodium perchlorate.


Human serum lipoproteins as inhibitors of haemagglutination for selected togaviruses.


Studies in genetic resistance to RSV (RAV-O). A third type of genetic resistance typified by the W line of fowl and further support for the two loci genetic model.


Proceedings: Interrelationship between swimming performance and chronological age, dental age, skeletal age of age group competitive swimmers.


On the nature of poliovirus genetic recombinants.


Retarded growth of poliovirus in contact inhibited cells.


A temperature-sensitive mutant of vesicular stomatitis virus with two abnormal virus proteins.


Bovine enterovirus-1: characterization, replication and cytopathogenic effects.


The effect of an antireceptor serum on mammalian cell lines.


Murine sarcoma virus-induced cytopathological effects in mouse cells made resistant to 6-azauridine.


A locus affecting circulating interferon levels induced by mouse mammary tumour virus.


Association of foot-and-mouth disease virus replicase with RNA template and cytoplasmic membranes.


A stable syncytial mutant of herpes simplex type 2 virus.


Heparin anticoagulation as adjuvant to chemotherapy in carcinoma of the lung.


Potential of yttrium-aluminum-garnet laser in caries prevention.


Effects of sodium warfarin and other carcinostatic agents on malignant cells: a study of drug synergy.


Ribonucleotides linked to DNA of herpes simplex virus type 1. Cells of a continuous cell line derived from rabbit embryo fibroblasts were infected with herpes simplex type 1 virus (HSV-1) and maintained in the presence of either [5-(3)H]uridine or [methyl-(3)H]thymidine or (32)PO(4) (3-). Nucleocapsids were isolated from the cytoplasmic fraction, partially purified, and treated with DNase and RNase. From the pelleted nucleocapsids, DNA was extracted and purified by centrifugation in sucrose and cesium sulfate gradients. The acid-precipitable radioactivity of [5-(3)H]uridine-labeled DNA was partially susceptible to pancreatic RNase and alkaline treatment; the susceptibility to the enzyme decreased with increasing salt concentration. No drop of activity of DNA labeled with [(3)H]thymidine was observed either after RNase or alkali treatment. Base composition analysis of [5-(3)H]uridine-labeled DNA showed that the radioactivity was recovered as uracil and cytosine. In the cesium sulfate gradient, the purified [5-(3)H]uridine-labeled DNA banded at the same position as the (32)P-labeled DNA. The present data tend to suggest that ribonucleotide sequences are present in HSV DNA, that they are covalently attached to the viral DNA, and that they can form double-stranded structures.


Immunochemical characterization of two major polypeptides from murine mammary tumor virus. A major murine mammary tumor viral (MMTV) antigen, sl, originally described by Nowinski et al. (1967, 1968, 1971), has been purified from RIII mouse milk MMTV by sequential ion-exchange and gel chromatography. The purified protein with sl antigenic reactivity contains carbohydrate, and has an apparent minimal molecular weight of 52,000. It can be designated as gp52 (sl). Another major MMTV viral protein with a molecular weight of 27,000 has also been isolated, and antisera have been prepared against it. Both MMTV gp52 (sl) and p27 viral polypeptides have been iodinated with (125)I and used in immunoprecipitation and competition assays. The two MMTV proteins differ absolutely from each other and from major mouse type C viral polypeptides in molecular weight, immunological reactivity, and amino acid composition. Purified gp52 (sl) in radioimmunoprecipitation inhibition assays reacted in two distinct patterns. One pattern showed partial displacement of antibody which could be converted to the second, a complete displacement, by heating the antigen, presumably by exposing additional reactive determinants. Biologically, the patterns of major MMTV polypeptide expression in milk correlated with spontaneous mammary tumor incidence in different strains of mice, indicating that the sl antigen is group specific for MMTV or that several mouse strains contain the same virus type.


Harvey sarcoma virus: a second murine type C sarcoma virus with rat genetic information. The nucleic acid sequences found in the Harvey strain of murine sarcoma virus have been analyzed by RNA.[(3)H]DNA and [(3)H]RNA.DNA hybridization techniques. The Harvey strain of murine sarcoma virus has been found to possess at least two sets of nucleic acid sequences. One set of sequences is contained in the Moloney strain of mouse type-C virus, and the other set is contained in DNA transcripts synthesized in endogenous reactions containing rat type-C virus(es). The nucleic acid sequences that are detected in the Harvey sarcoma virus with the DNA probes synthesized from the rat type-C virus(es) are related to the rat sequences detected in the Kirsten strain of murine sarcoma virus. The results support the model that both Kirsten and Harvey sarcoma viruses arose through a process of recombination or reassortment between mouse type-C viruses and sequences in rat cells and suggest that the information for transformation of fibroblasts may be contained in the rat type-C or cellular genome.


Conditional lethal mutants of adenovirus 2-simian virus 40 hybrids. I. Host range mutants of Ad2+ND1. Human adenovirus type 2 (Ad2) grows poorly in monkey cells, although this defect can be overcome by co-infection with simian virus 40 (SV40). The nondefective Ad2-SV40 hybrid virus, Ad2(+)ND1, replicates efficiently in both human and African green monkey kidney cells, presumably due to the insertion of SV40 sequences into the Ad2 DNA. Several mutants of Ad2(+)ND1 have been isolated that grow and plaque poorly in monkey cells, although they retain the ability to replicate and plaque efficiently in HeLa cells. One of these mutants (H39) has been examined in detail. Studies comparing the DNA of the mutant with Ad2(+)ND1 either by the cleavage patterns produced by Escherichia coli R.RI restriction endonuclease digestion or by heteroduplexing reveal no differences. The pattern of protein synthesis of Ad2(+)ND1 and H39 in monkey cells is quite different with the mutant resembling Ad2, which is defective in the synthesis of late proteins. However, in human cells, the proteins synthesized by H39 and the parent Ad2(+)ND1 are very similar. The production of SV40 U antigen in H39-infected cells is different from that in Ad2(+)ND1-infected cells. Finally, the growth of H39 in monkey cells can be complemented by SV40.


Inhibition of the multiplication of vesicular stomatitis and Newcastle disease virus by 2-deoxy-d-glucose. The production of infectious vesicular stomatitis (VSV) and Newcastle disease virus can be completely inhibited by 2-deoxy-d-glucose in pyruvate-containing medium, if virus either grown in pyruvate-containing medium or dialyzed against phosphate-buffered saline is used for infection. Under these conditions, the synthesis of all VSV proteins is reduced. VSV RNA, which is synthesized at reduced rates, seems to be unstable. The effect is completely reversible. If virus grown in glucose-containing medium is used for infection, the production of both viruses is not significantly inhibited by 2-deoxy-d-glucose. Under these conditions the production of the VSV glycoprotein is specifically impaired, but does not lead to a marked reduction of the yield of infectious virus.


Virion-bound protein kinase in Semliki forest and Sindbis viruses. Semliki forest virus and Sindbis virus (Alphaviruses belonging to the togavirus group) grown in BHK-21 cells possessed very low levels of virion-associated protein kinase activity. For comparison, vesicular stomatitis virus, also grown in BHK-21 cells, contained a virion-bound protein kinase which had a specific activity 80 times greater than that of the Alphaviruses. The Alphavirus protein kinase was unmasked by the nonionic detergent Nonidet P-40 but was not activated by cyclic nucleotides. Phosvitin was the best exogenous phosphate acceptor for assaying the viral enzyme in vitro. Phosphoprotein phosphatase activity was also detected in the Alphaviruses. Both in vivo and in vitro, all of the viral structural polypeptides were phosphorylated, and the phosphorylated amino acids were found to be serine and threonine. The viral nucleocapsid protein was about four times more efficient as a phosphate acceptor than were the envelope proteins. From 33 to 50% of the total protein kinase was bound to the viral nucleocapsid, and the specific activity of this enzyme was 4 to 10 times greater than that associated with the viral envelope.


Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. Transformation of hamster kidney cells by adenovirus 2 and the nondefective hybrid viruses. Nonhybrid adenovirus 2 (Ad2) and five nondefective Ad2-simian virus 40 (SV40) hybrids (Ad2(+)ND(1)-Ad2(+)ND(5)) failed to produce tumors when injected into newborn hamsters. However, each of these agents transformed hamster kidney cells in vitro, and the transformed cell lines produced tumors when injected into suckling hamsters. Foci of cells transformed by the hybrids could not be distinguished from foci of cells transformed by nonhybrid Ad2. Histopathologically, tumors induced by the nondefective hybrid-transformed lines were of the adenovirus type with no areas of SV40-type fibrosarcoma. All of the cell lines studied contained Ad2 T antigen and Ad2 RNA. Only the B55HK(1) line contained the three early SV40 antigens, U, TSTA, and T. The ND(4)HK(1) line contained SV40 T and TSTA antigens (the latter antigen being detected only after 48 to 49 tissue culture passages) and SV40 RNA. The ND(2)HK(2) line contained SV40 RNA but no detectable SV40 antigens; the other hybrid-transformed lines contained no SV40 RNA. The transformation of hamster cells by the nondefective Ad2-SV40 hybrids seemed to be caused by the Ad2 genome and could not be attributed to the regions of the SV40 genome contained within the hybrids. Additionally, these results indicate that interactions between the genomes of the nondefective hybrid viruses and the hamster cells they transform are complex and could involve the site of integration, the size of the incorporated viral genome, the transcription or translation of the viral genome, or various combinations of these processes.


Virion-associated RNA primer for Rous sarcoma virus DNA synthesis: isolation from uninfected cells. Uninfected chicken, duck, rat, and human fibroblast cells in culture contained a tRNA-like RNA molecule which was structurally identical to a virion-associated RNA primer for in vitro Rous sarcoma virus DNA synthesis. This primer RNA appeared to be a normal tRNA of these cells. It was not found in a number of lower eukaryotic cells or in Escherichia coli.


Endogenous C-type virus of a mouse cell line and its defectiveness. MLg cells originating from a newborn ddY mouse began to release C-type virus by treatment with 5-iodo-2'-deoxyuridine (5 mug/ml) for 48 h, or spontaneoulsy after prolonged cultivation. The data indicated the presence of three categories of the viruses spontaneously induced, i.e., virus equipped with the abilities of infecting and transforming the S+L- C182 cells, virus which can infect C182 cells but is unable to transform C182 cells, and virus which cannot infect nor transform the C182 cells, but is positive for the XC test. The type of the virus produced depended upon the cell clone. With the advance in the passage level, the cell population was dominated by the cells producing, uniquely, the last category of the virus.


Simian virus 40 transcription in productively infected and transformed cells. Several independent cell lines transformed by simian virus 40 carry a species of viral RNA of 900,000 to 1,000,000 daltons. A viral RNA species of similar size is found early in the lytic cycle. Late in the viral lytic cycle, two prominent viral RNA species of about 600,000 and 900,000 daltons are seen. The larger late species shares nucleotide sequences with, and is less stable than, the smaller. These RNA species are located in the cytoplasm of the infected cell. The regions of the viral genome coding for these RNA species are mapped by hybridization of lytic RNA species to fragments of the genome produced by cleavage with Haemophilus aegyptius endonuclease.


Cellular replication and the persistence of inducible RNA type C viruses. Cells of mouse and rat origin contain endogenous type C viruses. There are intrinsic regulatory factors which restrict the ability of different classes of these inducible viruses to persist in cells after chemical activation. The cellular block to certain viruses is shown to be overcome under conditions in which the virus-activated cells are allowed continued exponential growth. These findings may help to explain the high frequency at which replicating type C viruses are detectable in naturally occurring tumors of some species.


Inactivation of herpes simplex virus by concanavalin A. The infectivity of herpes simplex virus type 1 (HSV-1) was inactivated after treatment with either concanavalin A (ConA) or periodate. Phytohemagglutinin, wheat germ agglutinin, pokeweed mitogen, and neuraminidase failed to inactivate the virus. The effect of ConA could be specifically inhibited or reversed by the addition of alpha-methyl-d-glucoside or alpha-methyl-d-mannoside. Evidence was obtained that HSV-1 inactivated by ConA could adsorb to host cells. Viral aggregation was not a major mechanism in the inactivation of HSV-1 by ConA. Under the experimental conditions employed, inactivation of HSV-1 was faster by ConA than by antiserum and less temperature dependent. A ConA-resistant fraction was detected which appeared to adsorb less quickly than untreated virus, and penetration of ConA-resistant fraction was strikingly slow. The presence of aggregates in the virus preparation did not appear to account for the ConA-resistant fraction. Inactivation of viral infectivity by ConA was obtained only with enveloped viruses, since HSV-1, HSV-2, pseudorabies, and vesicular stomatitis virus were inactivated and vaccinia and echovirus type 6 were not.


Ocular myopathy. A case history with electron microscopy, biochemistry and review of literature.


Effect of cyclic AMP and some structural analogues on the dark repair inhibition by caffeine of uv-irradiated cells.


Effects of vestibular nuclei lesions on vestibulo-ocular reflexes and posture in monkeys.


Spindle responses in pig eye muscles.

Spindle responses in pig eye muscles.


Focal nodular hyperplasia of the liver. Possible relationship to oral contraceptives.


Letter: Ferruginous bodies in lung tissue.


Ten thousand consecutive frozen sections. A retrospective study focusing on accuracy and quality control.


The mechanism of interaction of red cell organic phosphates with hemoglobin.


Congenital band of the abdomen and the amniotic etiology of bands.


Colitis cystica profunda.

Colitis cystica profunda.


Serum lipids and lipoproteins in hepatosis of pregnancy and pre-eclampsia.


Rapid ACTH test with plasma aldosterone levels. Improved diagnostic discrimination.


Diagnostic use of glucagon-induced insulin response. Studies in patients with insulinoma or other hypoglycemic conditions.


Vincristine neurotoxicity.

Vincristine neurotoxicity.


Letter: "Swiss-cheese" platelets.

Letter: "Swiss-cheese" platelets.


Goltz syndrome with multiple giant-cell tumor-like lesions in bones. A case report.


Susceptibility of herpesviruses to cytosine arabinoside: standardization of susceptibility test procedure and relative resistance of herpes simplex type 2 strains. Susceptibility testing of herpes simplex type 1 (HSV-1) and type 2 (HSV-2) viruses to cytosine arabinoside (Ara-C) has been standardized under conditions of optimal reproducibility, susceptibility, and simplicity. Standard conditions were defined as virus inoculum of 10(1.5) to 10(2.5) TCID(50), WI-38 strain of lung fibroblasts, 2-day incubation, and observation of virus cytopathic effect after staining by the May-Greenwald-Giemsa method. The variability of minimum inhibitory concentration (MIC) of cytopathic effect was fourfold within each test and sixfold in successive assays. MIC values of all seven tested HSV-1 strains were in the range of 0.25 to 0.75 mug per ml, whereas four of nine HSV-2 strains had MIC values of 0.9 mug per ml and greater. A similar method can be used for assay of virus inhibitory titers of body fluids.


Letter: Gastrin and islet-cell tumors.

Letter: Gastrin and islet-cell tumors.


Analysis of 2-acetylaminofluorene: residues in laboratory chow and microbiological media.


A monomolecular electron transfer chain: structure and function of cytochrome C3.


Lividomycin resistance in staphylococci by enzymatic phosphorylation. Enzymatic inactivation of lividomycin (LV) was attempted with nine clinical isolates of staphylococci including LV-susceptible and -resistant strains. LV inactivation and the incorporation into LV of (32)P from gamma-(32)P-adenosine triphosphate were demonstrated in the presence of cell-free extracts from LV-resistant strains but not from LV-susceptible ones. The enzyme was purified approximately 82-fold from a resistant Staphylococcus aureus strain by means of ammonium sulfate fractionation and column chromatography. Some properties of the partially purified LV-phosphorylating enzyme were quite similar to those of an enzyme from Escherichia coli carrying an R factor conferring LV resistance, and the phosphorylated product of the drug was also found to be identical with that produced by E. coli carrying an R factor, i.e., 5''-phosphoryl-LV.


The variable course of primary hepatocellular carcinoma.


Sleep and delirium after open heart surgery.


Growth pattern and dietary intake of children with chronic renal insufficiency. The growth pattern and dietary intake of 33 children with varying degrees of renal insufficiency has been investigated. The development of impaired renal function in infancy has a more deleterious effect on linear growth than has its onset in later years. A reduction in growth velocity may occur once the glomerular filtration rate falls below 25 ml/min/1.73m(2). There was a significant reduction of the energy, protein, and vitamin D intakes of children with renal insufficiency compared with those recommended for their age, and of energy intake compared with that of normal children of their own height. Reduced growth velocity occurred when energy intake fell below 80% of that recommended. The reduction in the intake of energy and nutrients in these children may in part be responsible for their growth retardation.


Viability and pathogenicity of entomogenous fungi after prolonged storage on silica gel at -20degrees C.


Corynebacterium okanaganae, an entomopathogenic species of the Corynebacteriaceae.


Primary tumours of salivary glands: analysis of 111 tumours.


Conformational flexibility of the neurohypophyseal hormones oxytocin and lysine-vasopressin. A carbon-13 spin-lattice relaxation study of backbone and side chains.


Alteration of rabbit PCA reaction by drugs known to influence intracellular cyclic AMP.


Role of cyclic adenosine 3',5'-monophosphate in lymphocyte mitogenesis.


Spin-label studies of a hapten-combining site of rainbow trout antibody.


Properties of antisera to ganglioside GM1 and asialo GM1.


The variability of plasma oestradiol concentration in late pregnancy.


Increase in Gross (G) antigen sites on the surface of AKR virus-induced rat lymphoma cells after treatment with trypsin.


Adrenal response in pregnancy to long-acting tetracosactrin.


An evaluation of cytology in the diagnosis of herpes simplex virus infection and cytomegalovirus infection of the cervix uteri.


Synovial sarcoma of the cervical area.

Synovial sarcoma of the cervical area.


The cytidine mechanism and methylation pathway in the formation of N-methylated ethanolamine phosphoglycerides in Neurospora crassa.


Letter: On the repression of mitochondrial DNA transcription by fructose 1.6-diphosphate and its derepression by cyclic adenosine 3',5'-monophosphate.


Wilms's tumor in three children of a woman with congenital hemihypertrophy.


Differential labeling of triglycerides and polar lipids of cultured mammalian cells by albumin-bound (1-14C) fatty acids of serum.


Multiple denticle formation.

Multiple denticle formation.


Jaw metastases.

Jaw metastases.


A preview of growth hormone stimulation tests in children.


Tumor detection and localization with 99mTc-tetracycline.


Limitations of angiography and scanning in diagnosis of liver masses.


Studies on the mechanism of fructose-induced hyperuricemia in man.


Observations of altered intracellular phosphoribosylpyrophosphate (PP-ribose-P) in human disease.


Electrophoretic separation of normal and mutant phosphoribosylpyrophosphate synthetase.


Human erythrocyte phosphoribosylpyrophosphate content and "generation" during a constant rate infusion of xylitol.


Diagnostic value of Bekesy comfortable loudness tracings.


Malignant fibrous histiocytoma of the maxilla. A report of an unusual lesion.


Glomus tympanicum tumor in infancy.

Glomus tympanicum tumor in infancy.


Chemical, biochemical and toxicological differences between carbon tetrachloride and chloroform. A critical review of recent investigations of these compounds in mammals.


ATP balance and the effect of 2,4-dinitrophenol on fatty acid synthesis.


The sphingolipid composition of human renal carcinoma.


Isolation and characterization of an unsaponifiable lipid from Neurospora crassa.


The nuclear lipids of bovine hypertrophic thyroid.


The lipid composition of the salt (rectal) gland of spiny dogfish.


Interaction of I-anilino-8-naphthalene sulphonate with human serum low-density lipoprotein.


Stimulation by angiotensin of myocardial prostaglandin synthesis.


The rate of polypeptide chain elongation in a cell-free system from Krebs II ascites cells.


Modification of cytidine residues with a bisulfite-O-methylhydroxylamine mixture.


Proceedings: active enhancement of mouse skin allografts: the effect of Bordetella pertussis and regional lymphadenectomy.


Aetiology and prevention of wound infection in appendicetomy.


Proceedings: The effects of mercury perchloride solution on collagen synthesis in rat colon anastomoses.


Hydrolysis of bradykinin by angiotensin-converting enzyme.


Comparison between fractional turnover rate of endogenous plasma triglycerides and of intralipid (intravenous fat tolerance test) in man.


Proceedings: Comparison of 99Tc-labelled polyphosphate, pyrophosphate and ethane hydroxydiphosphonate in patients with skeletal metastases.


Plasma cyclic AMP and total catecholamines during acute myocardial infarction in man.


Sex difference in the kinetics of triglyceride metabolism in normal and hypertriglyceridaemic human subjects.


Relationship between plasma concentrations of angiotensin I, angiotensin II and plasma renin activity during cardio-pulmonary bypass in man.


Galactose metabolism in a patient with hereditary galactokinase deficiency.


Presence of the same types of nonhistone chromosomal proteins in different tissues.


Cycloheximide: a specific inhibitor of protein synthesis and intercellular ion transport in plant roots.


Muscarinic mediation of the biphasic temperature response to intrahypothalamic injections of carbachol in the cat.


The cyclic AMP response to noradrenalin in young adult rat brain following post-natal injections of 6-hydroxydopamine.


Carbohydrate components of plasma membrane of transitional epithelium of urinary tract.


A neuralizing influence of dibutyryl cyclic AMP on competent chick ectoderm.


Short term effects of dimethylnitrosamine and methylmethanesulphonate on hydrolases of the rat.


Use of restriction endonucleases in analyzing the genome of simian virus 40.


The usefulness of the relaxation response in the therapy of headache.


The Epstein-Barr virus and its relationship to infectious mononucleosis in Air Force recruits.


Clinical evolution of Japanese encephalitis.


Venereal disease in Vietnam: clinical experience at a major military hospital.


Association of the Golgi complex with the plasma membrane of amphibian embryonic cells.


Glomus tympanicum tumours: early diagnosis by arteriography.


Pediatric radiologic notes. Case no. 373. Mesenchymal hamartoma of the kidney in a neonate.


Visna virus-induced fusion of continous simian kidney cells.


Properties of afferent nerve impulses originating from a neuroma.


The blockade of angiotensin action by 8-leu-angiotensin on the guinea-pig aortic strip.


Drinking elicited by polyethylene glycol and isoproterenol reduced by antiserum to angiotensin II.


Thrombocythaemia. Familial occurrence and transition into blastic crisis.


Release and metabolism of prostaglandins in lung tissue.


A look at restraining practices and the use of psychotropic drugs.


Determination of the kinetic constants of fructose transport and phosphorylation in the perfused rat liver.


Which patient for which therapeutic group.


The action of melittin on phosphatide multibilayers as studied by infrared dichroism and spin labeling. A model approach to lipid-protein interactions.


At what level does the psychiatric clinical specialist practice?


Ultrasonic patterns of tumors of the liver.


Sterilization and therapeutic abortion counseling for the mentally retarded.


Everybody gets angry sometime.

Everybody gets angry sometime.


An expanded role for psychiatric nursing personnel: psychological evaluation and interpersonal care.


Proceedings: Pharmacological aspect of enzymes related to cyclic AMP.


Proceedings: Studies on food poisoning due to Clostridium welchii.


Prospects for contraception.

Prospects for contraception.


Proceedings: Inhibition of platelet cyclic AMP phosphodiesterase and platelet aggregation.


Nursing students and their learning experiences in geriatrics.


Proceedings: Effect of antidiuretic hormone on cyclic adenosine 3',5'-monophosphate levels in rat kidney cells in culture.


Proceedings: Fluorometric determination of cyclic AMP using dansyl chloride.


Proceedings: Effects of prostaglandin E on the inhibitory responses of taenia coli produced by electrical stimulation.


Speaking out on: approaching a sensible position on public facilities and treatment resources for the mentally ill and mentally retarded?


Proceedings: Pharmacological analyses of action of DBc-AMP on the central nervous system of conscious dogs.


Proceedings: Excretion of cyclic AMP into duct saliva from the salivary gland.


Proceedings: Comparison of cardiovascular effects of dibutyryl cyclic AMP (DBcAMP) and cyclic AMP (cAMP).


Proceedings: Effects of trazodone on monoamine metabolism in rat brain, as related to the paradoxical sleep suppressing effects.


Proceedings: Regional differences in the sensitivity to several bioactive peptides in the venous system of dog.


Proceedings: Physiological and pharmacological properties of bradikinin-sensitive dorsal horn neuron of the spinal cord.


Proceedings: Studies of the "whorled body" in the adrenocortical cell.


Proceedings: Effects of antispasmodics on phosphodiesterase.


Proceedings: Pharmacological studies on ganglionic transmission: effects of acetylcholine and catecholamines.


Proceedings: The effects of some drugs on the Ca++-uptake by microsomal fraction from guinea pig taenia coli.


Cellular and subcellular localization of 3H-oestradiol in the oviduct and uterus of the neonatal rat.


Detergent dispersion of adenylate cyclase from partially purified rat liver plasma membranes.


Transcellular fluid formation and vascular permeability increase by angiotensins.


Cyclic 3',5'-nucleotide phosphodiesterase. Distribution and developmental changes of the enzyme and its protein activator in mammalian tissues and cells.


Effect of temperature upon inhibition by substrate of lactate dehydrogenase isoenzymes from a poikilotherm.


Pilot experiments with EB virus in owl monkeys (Aotus trivirgatus). I Reticuloproliferative disease in an inoculated animal.


Primary immunodeficiency diseases and cancer: the immunodeficiency-cancer registry.


Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line.


Immunogenicity and immunosensitivity of SV40-transformed cells with different oncogenic potentials.


Analysis of minimal functions of simian virus 40. I. Oncogenic transformation of Syrian hamster kidney cells in vitro by photodynamically inactivated SV40.


Repair of radiation and chemical damage to DNA in human cells.


Triglyceride metabolism in skeletal muscle from normal and dystrophic mice.


Current concepts of therapy in mushroom intoxication.


Quinones of Brevibacterium.

Quinones of Brevibacterium.


Studies on the ribosomes of a thiopeptin-resistant mutant of Escherichia coli.


Isolation of cold-sensitive mutants of simian virus 40.


Intestinal resistance in the experimental enteric infection of mice with a mouse adenovirus. 3. Suppressive effect of cyclophosphamide on the establishment and duration of the intestinal resistance.


5-Keto-D-fructose. 8. Synthesis of 5-keto-D-fructose 1,6-bisphosphate and some of its properties.


Cyclic nucleotide levels in incubations of guinea pig trachea.


Regulation of gluconeogenesis by alanine.


Polysaccharides of the cellular slime mold. I. Extracellular polysaccharides in growth phase of Dictyostelium discoideum.


Modulation by cyclic AMP in vitro of liver plasma membrane (Na+--K+)-ATPase and protein kinases.


Disulphide groups of gonadotropin receptors are essential for specific binding of human chorionic gonadotropin.


Ultrastructlral and histochemical observations of the external epithelium of echinoderm tube feet.


Myodifferentiation in the carotid artery of the adult sheep: an ultrastructural study.


An electron microscopic study of the intraepidermal innervation of human glabrous skin.


Effect of glucose loading on blood sugar and on the histology of the pancreatic islets in Clarias batrachus (Linn.).


Avian pox in a mourning dove.

Avian pox in a mourning dove.


Anti-herpesvirus action of isoprinosine. Anti-herpesvirus effects of Isoprinosine were found to be condition-dependent in vitro and in vivo. A herpes type 1 strain, grown in primary rabbit kidney cells, was not susceptible to Isoprinosine when cytopathic effect was evaluated, but was modestly inhibited when plaque-forming methocel overlay was used in the same cell line. However, the growth of a herpes type 2 strain was inhibited by Isoprinosine in this system when cytopathic effect was evaluated. Both viruses produced an encephalitis in hamsters after application by corneal abrasion. The encephalitis produced by a 1.5 mean lethal dose of herpesvirus was strikingly suppressed by large doses of Isoprinosine given ad libitum in the drinking water; this protection was not seen, however, at 15 mean lethal doses. Anti-herpes effects of Isoprinosine were suppressed by additional treatment with cortisone, both in tissue culture and in vivo. Isoprinosine, in the absence of virus infection, produced significant effects on the metabolism, structure, and function of brain ribosomes. In vivo, RNA components were labeled more rapidly and gave up their radioactivity more slowly after treatment with Isoprinosine. This enhanced cytoplasmic radioactivity was associated with a loss of incorporated radioactivity located in the nucleus and an increase in radioactivity located in a ribonucleo-protein fraction >4S and <18S. Ribosomes from treated brains gave physical evidence of being more compact (less orthochromic). Isoprinosine treatment increased the incorporation of [(14)C]phenylalanine into nascent protein in a cell-free system examining protein synthesis in brain polyribosomes. The addition of the exogenous messenger ribonucleic acid, polyuridylic acid, produced a significant age-dependent inhibition in the incorporation of [(14)C] phenylalanine. This inhibition was significantly greater when ribosomes were isolated from animals pretreated with Isoprinosine. These and other observations suggest that the Isoprinosine stimulation of host ribonucleic acid metabolism may be coupled to the production of ribosomes that do not effectively translate exogenous messenger ribonucleic acid.


Susceptibility of 1,500 isolates of Pseudomonas aeruginosa to gentamicin, carbenicillin, colistin, and polymyxin B. A series of 1,500 strains of Pseudomonas aeruginosa was collected from a variety of sources to provide a group of strains which would truly represent the species. All of them were pyocine typed, and a wide range of types was included among the isolates from each source. The gentamicin, carbenicillin, colistin, and polymyxin minimal inhibitory concentration of each strain was measured by the agar dilution method employing the Steers inocula replicator. Over 99% of strains were inhibited by 8 mug of gentamicin per ml, by 256 mug of carbenicillin per ml, and by 4 mug of colistin per ml. The small number of strains more resistant to each antibiotic comprised a variety of different pyocine types. Few strains were found to be susceptible to tetracycline, chloramphenicol, kanamycin, or streptomycin at the single concentration tested.


Efficacy of trimethoprim and sulfamethoxazole in the prevention and treatment of Pneumocystis carinii pneumonitis. A combination of trimethoprim and sulfamethoxazole was effective in the prevention and treatment of Pneumocystis carinii pneumonitis in cortisonetreated rats. Although all of 15 untreated rats died with P. carinii pneumonitis, none of 15 given trimethoprim-sulfamethoxazole prophylactically acquired the infection. After P. carinii pneumonitis was established, 9 of 14 rats recovered after treatment with trimethoprim-sulfamethoxazole compared with only 2 of 14 treated with pentamidine isethionate. Rifampin and clindamycin, separately or in combination with pentamidine, were ineffective in the prevention and treatment of P. carinii infection.


Inhibition of Sindbis virus replication in HeLa cells by poliovirus. Concomitant infection of HeLa cells with poliovirus and Sindbis (SB) virus allowed replication of poliovirus only. The poliovirus-induced interference with the replication of SB virus was dependent partially on the multiplicity of SB virus used for infection. The observed interference was not sensitive to guanidine. Translation, but not replication of poliovirus, appears to be needed for the restriction of SB virus. Superinfection of SB virus-infected cultures with poliovirus was followed by inhibition of protein, but not by viral ribonucleic acid (RNA) synthesis. Polyribosomes present in SB virus-infected cells were disaggregated subsequent to superinfection. The nascent SB viral RNAs synthesized in cells subsequent to superinfection were not associated with the ribosomal structures. These results indicate that the block in protein synthesis, possibly at the level of initiation, may be the basis of the observed interference.


Discharge from hospital: the problem of adjustment.


Culture of hepatitis B virus on a human cell line of hepatic origin. Preliminary results.


Model studies in terpene biosynthesis.

Model studies in terpene biosynthesis.


Letter: Recognition of structural domains in globular proteins.


Unsuspected cytomegalic mononucleosis.

Unsuspected cytomegalic mononucleosis.


Specific immunodiagnosis of hepatocellular carcinoma by leucocyte adherence inhibition. The leucocyte adherence inhibition test provides a rapid, reliable, and specific technique for the immunodiagnosis of primary hepatocellular carcinoma (malignant hepatoma). The patient's blood leucocytes are tested in vitro for cell-mediated immunity against tumour-associated antigens and the serum is tested for blocking factor which interferes with the immunological reaction. Specific reactivity of both leucocytes and serum was consistently detected in patients with malignant hepatoma, and negative reactions were obtained in other liver diseases including secondary tumours of the liver. The test has provided positive evidence for the presence of hepatoma when more conventional methods gave doubtful or negative results. A positive result preceded the clinical appearance of tumour by up to three years in two patients.


Electrophysiological properties of the giant neurons of the leech subesophageal ganglion.


Morphometric study of the ultrastructure of human alcoholic fatty liver.


Calcium and Purkinje cells in a perfused frog cerebellum.


Recovery of neuronal activity and high-energy compound level after complete and prolonged brain ischemia.


Ultrastructure of chondrosarcoma.

Ultrastructure of chondrosarcoma.


Vascular lesions in the hand. Current management.


Photoconversions of bacteriochlorophylls and cytochromes in Chromatium chromatophores and cells under reducing conditions.


The transsynaptic relationship between dopamine and serotonin in the caudate nucleus of cats.


Inclusion of proteins of the cytoplasm of Zajdela hepatoma in the chromatin of rat liver cells.


Fine structure of rat islet cell tumors induced by streptozotocin and nicotinamide.


Aldosterone secretion in the enucleated rat adrenal during salt retention.


Prostaglandin receptors in the human, monkey and hamster uterus.


A comparison of cyclic nucleotide levels in plasma and cells of rat and human blood.


Proceedings: An explanation of the antiandrogenic properties of a non-steroidal compound, SCH 13521.


Prevention of illness from rhinovirus infection by a topical interferon inducer.


Proceedings: Thyroid-stimulating hormone binding to human thyroid membranes and solubilized receptors.


Proceedings: Corticosteroid feedback at the pituitary level.


Proceedings: Effect of human chorionic gonadotrophin on in-vivo levels of cyclic AMP in rabbit ovaries.


Proceedings: Pituitary adrenocorticotrophic activity after adrenalectomy and adrenal enucleation in the rat.


Hypothalamic control of gonadotrophin and prolactin secretion, implications for fertility control.


The gonadotrophins and their releasing factors.


Cholesteryl ester-rich inclusions from human aortic fatty streak and fibrous plaque lesions of atherosclerosis. I. Crystalline properties, size and internal structure. Cholesteryl esters, the major lipid in the lesions of human atherosclerosis, occurred in fatty streaks and fibrous plaques from 42 adults as spherical inclusions whose diameters averaged 1.99 +/- 0.48 and 2.16 +/- 0.62 mu, respectively, and which with polarizing light microscopy resolved into a mixture of various proportions of anisotropic and isotropic forms. Anisotropic forms had diameters of 1.9 +/- 0.4 mu, uniaxial-like symmetry with a formée cross image, a whitish gray interference color under cross polarizer and analyzer, second order blue and first order yellow interference colors with a tint plate, a birefringence of 0.0412 +/- 0.0054 and a refractive index of 1.559 +/- 0.0014. Their probable structural organization is primarily a lyotropic smectic mesophase (liquid crystal) of cholesteryl esters secondarily organized into multiple concentric lamellae. Isotropic forms had diameters of 2.3 +/- 0.6 mu and were optically inactive irrespective of their orientation. At 23 C, fatty streak inclusions from 21 subjects averaged significantly higher in the proportion of anisotropic forms, 85.5 +/- 9.3%, than did fibrous plaque inclusions from 21 subjects, which had 32.4 +/- 16.3% anisotropic forms (P < 0.01).


Hypoglycemia and insular hyperplasia: review of 148 cases. On the bases of personal experience and the series of 148 cases from an international inquiry and a review of the literature, the relation between insular hyperplasia and hypoglycemia was examined. The fundamental points in this investigation included: age, diagnostic investigations, histological findings and postoperative results. The data on the patients with insular hyperplasia indicates that 83% were of adult age. Insular hyperplasia was the only abnormal factor determined to be present. Through treatment a high percentage of cases (71%) was cured. From a practical surgical point of view, differentiation between occult insulomas and insular hyperplasia is not critical. Consequently, good results can be achieved in botlh diseases by practicing a progressive blind resection guided by examination of the operated specimen and by intra-operative blood sugar levels.


Responses to exogenous adenosine 3',5'-monophosphate of cardiac output and blood flow in the renal, superior mesenteric and carotid arteries in anesthetized dogs.


Effect of corticosterone and cortisol on corticosteroidogenesis in isolated rat adrenal cells.


Studies on plasma membranes. XXI. Inhibition of liver plasma membrane enzymes by tumour-promoting phorbolester, mitotic inhibitors and cytochalasin B.


Proceedings: Acute toxicity of cyclic AMP in mice.


Proceedings: Effect of calcium ion on renin release from renal cortex slices and isolated renin granules.


Proceedings: Metabolism of N6,02'-dibutyryl-3', 5'-cyclic-AMP (DBcAMP).


Nitrite in meat. Effect of various compounds on loss of nitrite.


Proceedings: Influence of morphine on ACTH secretion in the rat.


Proceedings: Effects of cholinergic drugs on the synaptic transmission in isolated spinal cord-nerve-muscle preparation of the bullfrog.


Response of plasma aldosterone to postural change, diuretica, angiotensin II, sodium restriction or loading and prostaglandin.


Proceedings: Mechanism of relaxation of smooth muscle by 3-isopropyl-5,7-dipiperidinomethyltropolone hydrochloride (IDPT).


Coronavirus infections in military recruits. Three-year study with coronavirus strains OC43 and 229E.


The effects of sodium chromate on the proximal tubules of the rat kidney. Fine structural damage and lysozymuria.


Melanin: a normal component of human eosinophils.


Effect of renin immunization on mercuric chloride and glycerol-induced renal failure.


Letter: Down's syndrome, hypothyroidism, and diabetes mellitus.


Temperature-sensitive pleiotropic revertants from a mutant in the arom gene cluster of Neurospora crassa. Genetical and biochemical studies have been performed with revertants induced in a polyaromatic mutant (No. 58) in the arom gene cluster of Neurospora crassa. In addition to complete and partial revertants able to grow on minimal at both 25 degrees and 35 degrees , temperature-sensitive revertants capable of growth on minimal at 25 degrees but not at 35 degrees have been recovered. One of these revertants has been shown to lack biosynthetic dehydroquinase activity at both temperatures (utilizing the inducible catabolic isozyme for growth at 25 degrees ), to have dehydroshikimate reductase activity only at 25 degrees , and to form an arom aggregate having a molecular weight approximately one-half that of wild type. These results are interpreted as indicating that pleiotropic mutants in the arom gene cluster can result from missense mutations, as well as from nonsense mutations as indicated in previous studies.


Malignancy in pleomorphic adenoma. A clinical and microspectrophotometric study.


Diagnostic tests of facial nerve function.


Neoplastic involvement of the facial nerve.


Intracellular hydroxylation of non-helical protocollagen to form triple-helical procollagen and subsequent secretion of the molecule.


Reference values for cord blood lipid and lipoprotein concentrations.


Mössbauer effect and electron spin resonance of the (iron)4-sulfur clusters of ferredoxin from Clostridium pasteurianum.


Chromatographic analysis of aminoacyl tRNAs of poliovirus infected HEp-2 cells.


Studies on seed-oil triglycerides. Triglyceride biosynthesis and storage in whole seeds and oil bodies of Crambe abyssinica.


Protein kinase activity in African swine fever virus.


Prostaglandins and inflammation.

Prostaglandins and inflammation.


Studies of glutamate dehydrogenase: chemical modification and quantitative determination of tryptophan residues.


Comparison of the PGE1 and catecholamines influence on the active transport of sodium.


Foot-and-mouth disease virus: plaque reduction neutralization tests.


Hemadsorption cell-counting assay of interferon.


ATPase and GTPase activities isolated from rat liver ribosomes.


Neutralizing activity in the serum and oesophageal-pharyngeal fluid of cattle after exposure to foot-and-mouth disease virus and subsequent re-exposure.


The reactivation of C55-isoprenoid-alcohol-phosphokinase apoprotein by lipids. Evidence for lipid hydration in lipoprotein function.


The intracellular location of pyruvate carboxylase in rat liver.


The surface energetics of asbestos minerals.


Adenosine 3',5'-cyclic phosphate, lipolysis and oxygen consumption in brown adipose tissue from newborn rabbits. Effect of cervical sympathectomy and of noradrenalin and theophylline in vitro, and the partial purification of an adenosine 3',5'-cyclic phosphate-dependent protein kinase.


Enhancement of the sensitivity of the immunodiffusion reaction in the detection of antibody against bovine adenoviruses.


Enhancement in excitability properties of mouse neuroblastoma cells cultured in the presence of dibutyryl cyclic AMP.


Aromatic sulfonic acids as viral inhibitors. Structure-activity study using rhino, adeno 3, herpes simplex, and influenza viruses.


Angiotensin II analogs. 5. (2-Glycine)angiotensin II and related analogs.


Septic arthritis of the hip joint secondary to umbilical artery catheterization associated with transient femoral and sciatic neuropathy.


Potential antidiabetics. 9. Biological activity of some pyrazoles.


Studies of malformation syndromes of man 33: the FG syndrome. An X-linked recessive syndrome of multiple congenital anomalies and mental retardation.


Protein kinase-catalyzed phosphorylation of muscle sarcolemma.


Control of respiration by the mitochondrial phosphorylation state.


Differences in active center reactivity of trypsin homologs. Specific inactivation of thrombin by nitrophenyl p-amidinophenylmethanesulfonate.


Hyperlipoproteinemia type V: biochemical observations in two cases.


Distribution of enzymes of glycogen metabolism and calcium uptake in skeletal muscle.


Glucosylceramide in plasma of patients with Niemann-Pick disease.


The use of synthetic inhibitors to study the interaction of nicotinamide adenine dinucleotide with yeast alcohol dehydrogenase.


The renin-angiotensin system in the fetal lamb.


Biochemical changes during progesterone-induced maturation in Xenopus laevis oocytes.


Enzyme catalysis: conflicting requirements of substrate access and transition state affinity.


Receptor-binding assay of chemically modified insulins. Comparison with in vitro and in vivo bioassays.


Ocular manifestations of systemic disease.


Plasma cyclic adenosine 3'5'-monophosphate (AMP) levels in acute myocardial infarction.


Donovanosis: a morphologic study.

Donovanosis: a morphologic study.


The maintenance of liver cells in vitro for virological and other studies.


The adrenergic innervation of the gastrointestinal tract.


Letter: Postmortem formation of giant endothelial vacuoles in Schlemm's canal.


Ultrastructural and histochemical studies of a newly recognized form of systemic mucopolysaccharidosis. (Maroteaux-Lamy syndrome, mild phenotype).


Effect of N-methyl-N-nitrosourea on the protein-synthesizing system in mouse liver and hepatoma 22a cells.


Different degradation rates of alkylated RNA protein and lipids in normal and tumor cells.


The catabolism of alpha1-fetoprotein and albumin in rats bearing Morris hepatoma 7777.


Diurnal distribution of motor activity and feeding during growth of tumors.


Cytoplasmic glucocorticoid-binding proteins in glucocorticoid-unresponsive human and mouse leukemic cell lines.


A comparison of phenylalanyl-tRNA synthetase from rat liver and a minimal deviation hepatoma.


Evidence for bile acid synthesis by transplantable hepatomas.