Properties of nucleoprotein complexes containing replicating polyoma DNA. Short-lived nucleoprotein complexes (r-py complex) containing replicating polyoma DNA were isolated from infected cells after lysis with Triton X-100. The Triton lysing procedure of Green, Miller, and Hendler (1971) releases most complexes containing supercoiled viral DNA (py complex) from nuclei, but liberates only a portion of r-py complexes. r-py Complexes are associated more strongly with nuclear sites but can be extracted by prolonged incubation of nuclei in lysing solution. Complexes containing replicating polyoma DNA appear to be precursors to stable complexes containing supercoiled DNA. Sedimentation and buoyant density studies indicate that protein is bound to both r-py complexes and py complexes at a ratio of protein to DNA of about 1 to 2/1. Both types of complexes sediment as if the viral DNA is more compact than free DNA and both undergo major reversible configurational changes with increased salt concentration. Changes resulting from enzymatic and chemical treatment indicate that there may be two or more protein components in both r-py complex and py complex. One component is digested by Pronase and trypsin while another is resistant to the enzymes but released by deoxycholate. The abundance and similarity in chemical and physical properties of protein bound to all forms of polyoma DNA suggest that part of the protein molecules may serve in a structural capacity.


Nucleoprotein complexes containing replicating Simian virus 40 DNA: comparison with polyoma nucleoprotein complexes. Procedures for isolating nucleoprotein complexes containing replicating polyoma DNA from infected mouse cells were used to prepare short-lived nucleoprotein complexes (r-SV40 complexes) containing replicating simian virus 40 (SV40) DNA from infected monkey cells. Like the polyoma complexes, r-SV40 complexes were only partially released from nuclei by cell lysis but could be extracted from nuclei by prolonged treatment with solutions containing Triton X-100. r-SV40 complexes sedimented faster than complexes containing SV40 supercoiled DNA (SV40 complex) in sucrose gradients, and both types of SV40 nucleoprotein complexes sedimented ahead of polyoma complexes containing supercoiled polyoma DNA (py complex). The sedimentation rates of py complex and SV40 complex were 56 and 61S, respectively, based on the sedimentation rate of the mouse large ribosomal subunit as a marker. r-SV40 complexes sedimented as multiple peaks between 56 and 75S. Sedimentation and buoyant density measurements indicated that protein is bound to all forms of SV40 DNA at about the same ratio of protein to DNA (1-2/1) as was reported for polyoma nucleoproteins.


Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. I. Sequence of viral replication. A comparison, under standardized conditions, of herpes simplex virus (HSV) and human cytomegalovirus (CMV) revealed differences in viral morphology, in the timing of their infectious cycles, and in several morphological events during those cycles. Structural distinctions between the two viruses included the coating of unenveloped cytoplasmic CMV capsids, but not those of HSV, and a variation in the structure of their cores. Since the two cycles were carried out in the same host cell strain under conditions of one-step growth (input multiplicity = 10 PFU/cell), it was possible to construct time scales locating the major events of each cycle. Comparison of the two showed that HSV replicated and released progeny within 8 h postinfection, whereas CMV required 4 days. These results correlated well with those of concurrent plaque assays. Within the longer CMV cycle, most of the major events appeared retarded to a similar degree, and no obvious limiting step in particle production could be identified. Distinctions between the two cycles included the following: condensation of the chromatin in HSV- but not CMV-infected cells; the greater tendency of HSV to produce membrane alterations; and the appearance of cytoplasmic dense bodies in CMV- but not HSV-infected cells. Identification of these differences even under identical conditions of culture and infection strongly implies that they result from intrinsic differences in the nature of the viruses, and are not caused by variations in experimental conditions.


Properties of noninfectious and transforming viruses released by Murine sarcoma virus-induced hamster tumor cells. The cell culture lines HTG2 and HTG3 were established from a transplantable hamster tumor induced by a murine sarcoma virus (strain Gz-MSV) after 17 and 60 in vivo passages, respectively. The viruses released by these two cell lines markedly differ in morphology, antigenic composition, infectivity, transforming ability, and enzymatic activity. HTG2 virions contained the sarcoma genome but were noninfectious for mouse and hamster cells (S+H-virus). HTG3 virions transformed hamster but not mouse cells. Whereas HTG2 cells and its virus contained murine type C virus gs-1 antigen, all HTG3 clonal lines expressed both murine and hamster type C virus gs-1 antigens. The RNA-dependent DNA polymerase activity of HTG2 virus was very low, whereas that of HTG3 virus was relatively high. HTG2 virions contained electron-lucent centers only. HTG3 virus consisted of the expected mixture of virions with electron-dense and electron-lucent centers. Many broken or incomplete virions were present in both viruses. HTG2 virus is a noninfectious "defective" sarcoma virus without detectable helper virus. Data obtained in these experiments suggest that HTG3 virus is a hamster type C virus pseudotype of Gz-MSV (Gz-MSV [HaLV]). The genome of Gz-MSV is capable of antigenic expression in heterologous cells and in the presence of heterologous viruses. Attempts to chemically activate hamster type C virus (HaLV) from HTG2 cells were unsuccessful. The HTG1 cell culture line, established from another Gz-MSV-induced hamster tumor, initially released a virus indistinguishable from the HTG2 virus. After in vitro passage, spontaneous activation of HaLV occurred in HTG1 cells, and the resultant Gz-MSV (HaLV) had properties similar to those of the HTG3 virus.


Factors affecting cell fusion induced by Sendai virus. Cell fusion mediated by exogenous Sendai virus appears to occur in four temperature-dependent stages. The first two, which include viral adsorption, are pH dependent and can be inhibited by viral antibody. Viral envelope constituents remain detectable on the cell surface during the third stage and disappear only when cell-to-cell fusion supervenes. The relationship of these interactions to possible mechanisms of cell fusion are discussed.


Induction of cellular DNA synthesis by the nondefective Adenovirus 2-Simian virus 40 hybrid viruses. Induction of cellular DNA synthesis by the nondefective adenovirus 2-simian virus 40 hybrid viruses was examined in monkey and hamster cells. It was not possible to discriminate the effects of the hybrid viruses from those of wild-type adenovirus 2.


Denitrification in granular carbon and sand columns.


Health-services information systems in the United States today.


Defective superoxide production by granulocytes from patients with chronic granulomatous disease.


Letter: Acute hemorrhagic cystitis due to adenovirus Type 11.


Renal function in pregnancy.

Renal function in pregnancy.


Influence of malignant cells on the healing of colonic anastomoses: experimental observations.


Generalized eruptive histiocytoma.

Generalized eruptive histiocytoma.


Toward a theory of dream recall.

Toward a theory of dream recall.


The sodium story: effects of anaesthesia and surgery on intrarenal mechanisms concerned with sodium homeostasis. Enormous progress has been made in the last 10 years toward an understanding of sodium homeostasis by the kidney.  Much of the relevant literature is reviewed.  Anesthesia and surgery are normally accompanied by severe sodium retention.  2 studies which investigated this area are summarized.  Together they demonstrate the importance of the kidney in monitoring an individual's hemodynamic state and adjusting his sodium excretion accordingly.  1 study showed uniform fluid retention following surgery, even in patients receiving high levels of extra fluid.  Water and sodium retention were shown to be responses to different, unrelated mechanisms.  Plasma or extracellular fluid deficits were shown to be unrelated to the amount of retention.  These 2 studies confirmed an earlier hypothesis by de Bono and Mills that changes in renal hemodynamics may be responsible for changes in sodium excretion.  Actually, a number of substances, all with powerful vasoactive properties, have been isolated in the kidney.


Adrenalectomy for metastatic carcinoma of the breast. Experience in a community hospital.


1971 Yant Memorial Lecture. Mining: problems and progress in industrial hygiene.


Adenine arabinoside therapy in corneal stromal disease and iritis due to herpes simplex.


Effect of oestradiol-3,17beta dipropionate on spermatogenesis and lysosomal enzymes in the rat testis.


Inhibition by plant growth retardants of cholesterol biosynthesis in slices of rat liver and hepatoma.


Experience with a short test of pituitary-adrenal function.


A passive haemagglutination test for the demonstration of antibody to infectious bovine rhinotracheitis-infectious pustular vulvovaginitis virus. 2. Studies on antibody incidence and the serological response after infection.


Cyclic 3',5'-adenosine monophosphate dependent protein kinase from dog prostate.


Effect of EGTA on protein release and cyclic AMP accumulation in rat parotid gland.


Comparative aspects of equine herpesviruses.


Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm.


The physiology of prostaglandins.

The physiology of prostaglandins. This is a review from the literature dealing with the physiology of prostaglandins (PGs).  PGs E and F are biosynthesized from straight chain C-20 fatty acids, arachidonic acid being the precursor of 2 of the series and dihomo-linolenic acid the precursor of the other series.  Availability of certain cofactors during synthesis probably determines which PG is formed.  The amounts of PGs in the tissues are very low, indicating that they are biosynthesized immediately before hormone-stimulated release.  PGs seem to be metabolized by all body tissues.  PGE and PGF cannot act as circulating hormones since they are 95-99% deactivated by 1 passage of the blood through the lungs.  The following roles of PGs in the reproductive process are discussed:  1) present in semen, they may aid in fertilization; 2) they may function as mediators between luteinizing hormone and cyclic AMP during ovulation; 3) they may facilitate release of anterior pituitary hormones; 4) they act to stimulate progesterone secretion; 5) they act in some species as the uterine luteolytic hormone; and 6) they sensitize the uterus to oxytocin during delivery.  Since every body tissue appears to be able to synthesize PGs, they also have other physiological functions.


Studies on 1-beta-D-arabinofuranosyl-cytosine-resistant mutants of Chinese-hamster fibroblasts. A mitochondrial deoxycytidine kinase devoid of activity on arabino-cytosine.


The treatment of some neoplastic diseases in children with a new cytostatic drug: peptichemio. Investigations of a possible interference with lymphocyte blastogenesis and chromosomes.


Effects of 5'-amido analogues of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphothioate on protein kinase, binding protein and phosphodiesterases.


2-Amino-2,6-dideoxy-d-glucose (D-quinovosamine): a constituent of the lipopolysaccharides of Vibrio cholerae.


A nuclear-magnetic-resonance study of the manganese-thiamine-pyrophosphate complex in solution.


Studies on the induction of nitrate reductase by nitrite in bean-seed cotyledons.


Clinical evaluation of indoramin, a new antihypertensive agent.


The lactose system in Klebsiella aerogenes V9A. 3. Specific repression of the lac operon by melibiose and raffinose.


Suspected adenoviral infection in adult dairy cattle.


Effects of bovine viral diarrhea and parainfluenza-3 virus vaccines on development of respiratory tract disease in calves.


Big ACTH: conversion to biologically active ACTH by trypsin.


Metabolism of endogenous proinsulin and insulin in man.


Insulin receptors in the plasma membranes of human placenta.


Cyclic adenosine monophosphate stimulates secretion of human chorionic gonadotropin and estrogens by human trophoblast in vitro.


Complementation of translational defect for growth of human adenovirus type 2 in Simian cells by a Simian virus 40-induced factor.


Rotational relaxation time of concanavalin A bound to the surface membrane of normal and malignant transformed cells.


Selective inhibition of aldosterone synthesis by 11-hydroxylated spirolactone in rat adrenals.


Neurotransmitter uptake: a tool in identifying neurotransmitter-specific pathways.


Nerve sheath myxoma in the tongue.

Nerve sheath myxoma in the tongue.


Surgical management of recurrent head and neck cancer.


Radiotherapy for postoperative persistent cancer of the head and neck.


The sensitivity to 5-hydroxytryptamine of various parts of the gastro-intestinal tract of the rats with experimental gastric ulcers.


Cryosurgery for recurrent cancer of the head and neck.


Respiratory symptoms and rash in mumps.

Respiratory symptoms and rash in mumps.


Experimental silicosis. The relation between dose of quartz, length of action for the dust, tissue reaction and disturbance of the lung function.


Renal hypertension in children.

Renal hypertension in children.


Aromatization of the A-ring of norethynodrel, a steroidal oral contraceptive, during trimethylsilylation. Treatment of the steroidal oral contraceptive, norethynodrel (Ia), under persilylation conditions with trimethylsilylimidazole yielded two products: 17 alpha-ethynyl-3,5(10)-estradien-3, 17 beta-diTMS (IIa) and 17  alpha-ethynyl-1,3,5(10)-estratrien-3,17 beta-diTMS (IIIa).  Extended reaction time at 100 degrees C yielded only IIIa, the aromatic substance.  A second steroid, 5(10)-estren-17 beta-ol-3-one (LB), gave the corresponding (IIb (iib and IIIb).  Both aromatic compounds were characterized by gas chromatography-mass spectrometry comparisons with the diTMS-derivatives of the corresponding estradiols.  The mechanism of the aromatization reaction is unknown, but was probably one of dehydrogenation rather than of oxysilylation followed by loss of trimethylsilanol and aromatization.


Effects of isoflurane and halothane on contractility and the cyclic 3', 5'-adenosine monophosphate system in the rat aorta.


Pregnancy in a patient with hormone deficiency.


Capacitance, area, and thickness variations in thin lipid films.


Proceedings: Correlations between certain biochemical properties of breast cancer and response to therapy: a preliminary report.


Proceedings: Primary oropharyngeal cancer and hypercalcemia.


Proceedings: Immunotherapy of malignant disease: the use of viable sensitized lymphocytes or transfer factor prepared from sensitized lymphocytes.


Proceedings: Treatment of minimal breast cancer.


Proceedings: Primary liver cancer. A review of the clinical features, blood groups, serum enzymes, therapy, and survival of 65 cases.


Continuing surveillance of families for studying the epidemiology of viral infections.


Survey of recent literature on the menstrual cycle and behavior.


Ultrastructural studies of eight cases of fetal Tay-Sachs disease. 7 fetuses of 27 high risk pregnancies showed absence of hexosaminidase A and were aborted.  In all 7 cases, biochemical examination of fetal tissue confirmed the diagnosis.  In the brains of the Tay-Sachs disease fetuses electronmicroscopy showed an increase in G-M2 ganglioside and absence of hexosaminidase A.  By 12 weeks gestation abnormal cytoplasmic inclusions were present in the spinal cord.  By 22 weeks, ganglion cells of the retina and spinal ganglia showed alteration of cellular organelles and abnormal lipid material in the cytoplasm.  Attempts at enzyme replacement were unseccessful.


The pathogenesis of cholera and some wider implications.


The type II epithelial cell of the lung. I. Method of isolation.


Enzymatic solubilization and separation of glomerular collagen and sialoprotein. Effect of contaminating enzymes present in commercial collagenase preparations.


Erythrocyte enzymes and glycolytic intermediates of high- and low-glutathione sheep.


Dehydration of macromolecules. II. Protective effects of certain anions on ribulosediphosphate carboxylase subjected to low water potenials in vitro.


Effect of naftidrofuryl upon energy metabolism of the brain.


Biological implications of the instability of the N-glycosidic bone of 3-methyldeoxyadenosine in DNA.


On the binding and specificity of 3,6-bis-(acetatomercurimethyl)-dioxane to DNAs of different base composition.


Comparison of the acceptance activity of the ribosome-bound and the total cellular transfer ribonucleic acids from SV40-transformed mouse fibroblasts.


Receptor mechanism concepts.

Receptor mechanism concepts.


The effect of N6-2'-O dibutyryl 3',5' cyclic adenosine monophosphate, imidazole and aminophylline on ganglionic transmission in the superior cervical ganglion of the cat. 1. Dibutyryl cyclic AMP, injected towards the superior cervical ganglion of the cat, produced no consistent responses.2. Imidazole, injected towards the ganglion, regularly produced facilitation, both during intermittent and continuous preganglionic stimulation. This effect was dose-dependent and lasted 2-10 minutes.3. Aminophylline, injected towards the ganglion, regularly produced depression of ganglionic transmission, both during intermittent and continuous preganglionic stimulation. This effect was also dose-dependent and lasted 1-4 minutes. Papaverine produced the same type of response as aminophylline.4. Imidazole potentiated the ganglionic response to 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), while aminophylline depressed it.5. The results of the present experiments are consistent with the view that cyclic AMP may have a mediating role in the process of ganglionic transmission.


Angiotensin I as a dipsogen: efficacy in brain independent of conversion to angiotensin II.


Vasopressors in obstetrics.

Vasopressors in obstetrics.


Antibiotics.

Antibiotics.


Rapid gas chromatographic assay for dehydroepiandrosterone in urine.


A program for assessing the effect of treatment on growth and development in children with growth disorders.


Chromatin, and gene regulation in eukaryotic cells at the transcriptional level.


Succinate dehydrogenase and cytochrome C oxidase activity of antigen stimulated macrophages.


Surface biochemical changes accompanying primary infection with Rous sarcoma virus. I. Electrokinetic properties of cells and cell surface glycoprotein:glycosyl transferase activities.


Nuclear cytochemical changes in rat adrenal glands stimulated by adrenocorticotrophic hormone.


Periodate-induced lymphocyte transformation. 3. Effects of temperature and pH.


Surface biochemical changes accompanying primary infection with Rous sarcoma virus. II. Proteolytic and glycosidase activity and sublethal autolysis.


Reactivation of chick erythrocyte nuclei in heterokaryons with rat hepatoma cells.


The relationship between cell growth, macromolecular synthesis and poly ADP-ribose polymerase in lymphoid cells.


Heavy metals and spermatozoan motility. II. Turbidity changes induced by divalent cations and adenosinetriphosphate in sea urchin sperm flagella.


Dipyrone as a cause of drug rashes: an epidemiologic study.


Cytochrome oxidase activity of mitochondria in sensory nerve endings of mouse palatal rugae.


Clinical pathological conference. The man without history.


Different binding of RNA polymerase to individual promoters.


Mutants of Escherichia coli unable to metabolize cytidine: isolation and characterization.


Melanotic neuroectodermal tumor of infancy. A case report and review.


Protocollagen proline hydroxylase activity in human dental pulp at various stages of development.


Endotoxin-induced alterations in isolated fat cells: effect on norepinephrine-stimulated lipolysis and cyclic 3',5'-adenosine monophosphate accumulation.


Peripheral vascular actions of alpha-methyl-DOPA and its mode of action on arterioles.


Isolated adrenal cortex cells: ACTH4-23 (NH2), ACTH5-24, ACTH6-24 and ACTH7-23 (NH2); cyclic AMP and corticosterone production.


Characterization of gluconeogenesis in enzymatically isolated parenchymal cells of rat liver.


The everted rat intestine used as a model system to demonstrate regulation of sodium absorption following hemorrhage.


Calcium-secretagogue interaction in the stimulation of gastric acid secretion.


Radiation-enhanced survival of a human virus in normal and malignant rat cells.


Correlation of C-type virus (WF-1) production and heme synthesis in a rat fibroblastic cell line.


Effect of glucosamine administration in normal rats in comparison with streptozotocin treatment.


Regulation of gingival collagenase: a possible role for a mast cell factor.


The effect of cold on the composition of the phospholipids of the blood plasma of healthy athletes.


Functions of the mammalian cerebral cortex in movement.


Heterogeneity of enzymes in leukaemic eosinophils.


Studies on the neural control of the skating reflex, and the origin of its variations in field population of the hemipterous insect Gerris.


Cyclic adenosine 3',5'-monophosphate dependent protein kinase of human platelets: membrane phosphorylation and regulation of platelet function.


Interactions of the pharmacological receptors of blood platelets with adenylate cyclase.


The release reaction: is it a common mechanism in all types of hemostatic cells?


Neonatal familial type II hyperlipoproteinemia: cord blood cholesterol and follow up study in 1.323 births.


Long term follow-up of the paced patients.


Obstructive uropathy secondary to calcified metastatic adenocarcinoma of colon.


Bilateral Wilms' tumor. Treatment, management, and review of the literature.


A micro assay for mouse and human interferon. II. Dose-response in different in cell-virus systems.


Immunofluorescence measurement of C1 inactivator (alpha 2 neuraminoglycoprotein) activity of the surface of human carcinoma cells.


Stability of human leukocyte interferon towards heat.


Diagnostic scoring system for mammary carcinoma.


Lymphoid cells in carcinoma of the breast. Failure of response to phytohaemagglutinin in vitro.


Amyotrophic lateral sclerosis. Electrophysiologic study (number of motor units and rate of decay of motor units).


Multiple muscle analysis of motor units in muscular dystrophy.


Hereditary brachial neuropathy. Report of two families.


Folate deficiency and neurological disease. One case with a close association.


Interspecies infection by feline leukemia virus: serial cell-free transmission in dogs of malignant lymphomas induced by feline leukemia virus.


Burkitt's lymphoma: cell kinetics, treatment and immunology.


Viral envelope antigens of the viruses of feline leukemia-sarcoma complex.


Significance of leukemia in animals for the human disease.


Structural and serological studies on animal 'C-type particles' and their implications for demonstrating corresponding viruses in man.


Studies on the relationship of RNA viruses to the origin of leukemia and solid tumors of animals and man.


Electron microscopy of feline leukaemia virus RNA.


Electron microscopy of early interactions between feline leukaemia virus and cells in tissue culture.


Role of C-type virus in bovine lymphosarcoma.


Continuing studies of feline sarcoma virus-induced tumors in nonhuman primates.


Biological studies in four species of nonhuman primates with simian sarcoma virus (Lagothrix).


A comparative study in vitro of a simian virus isolated from spontaneous woolly monkey fibrosarcoma and of a known feline fibrosarcoma virus.


The epidemiologic and genetic aspects of an outbreak of leukemia among Hamadryas baboons of the Sukhumi monkey colony.


Simian sarcoma virus: oncogenicity, focus assay, presence of associated virus, and comparison with avian and feline sarcoma virus-induced neoplasia in marmoset monkeys.


Structural components of a murine leukemia virus and the plasma membrane of virus infected cells.


An analysis of antigens on the surface of murine leukemia viruses and cells.


Etiological strain specificities of the avian tumor viruses.


High resolution electron microscopy studies of murine leukemia viruses.


Transmission and expression of the leukemia introducing agent in BALB-cf(R3) mice.


Demonstration of helper activity with human leukemic tissue extracts.


Studies of interferon and murine leukemia sarcoma virus group.


Spleen focus assays with a mouse leukemia virus in mice treated with actinomycin D, L-asparaginase or rifampicin.


In vitro transformation of leukocytes with a guinea pig herpes-like virus.


Effects of STAS JLSV5 and silicotungstate on the replication of murine sarcoma virus (Moloney) and poliovirus.


Immunizing and interfering action of non-leukemogenic C-type murine virus variants in leukemogenesis.


Studies on oncogenic properties of Epstein-Barr virus.


Persistence of Epstein-Barr virus DNA in human tumor cells.


The possible significance of morphological transformation of human fibroblasts by EB virus in vitro.


Purification of Herpesvirus saimiri DNA.


A prospective sero-epidemiological study to investigate the role of EBV in Burkitt's lymphoma.


Studies on the interrelationship of type B and type C virus particles in breast cancer and in leukemia.


Minor base changes in transfer ribonucleic acid in avian myeloblastosis virus.


The 60-70S RNA of avian sarcoma and leukosis viruses--distribution of class a and b subunits.


Presence of avian leukosis virus genes, in DNA form in normal chickens.


A comparative study of Marek's disease virus proteins.


Chromosomes of viral-transformed BALB-c-3T3 cells.


Possible activation of C-type particles during 'progression' of the chemically-induced pulmonary tumors in mice.


RNA tumor virus antigen expression in chemically-induced tumors. Significance of specific chemical carcinogens to the derepression of the C-type RNA virogene and oncogene expressions.


Isolation and characterization of the hamster C-type viruses.


Chemical co-carcinogenesis: differential action of various compounds, derepression of endogenous C-type RNA genomes, and influence of different genotypes of mice.


RNA tumor virus genomes as determinants of chemically-induced transformation in vitro.


In vitro carcinogenesis studies: dual effect of RNA tumor viruses and carcinogenic chemicals or DNA viruses.


Influence of immune suppression on the induction of neoplasms by MLV and MSV.


The role of immunity in radiation leukemogenesis.


Homologous and heterologous immunization against Rous sarcoma.


Isolation and characterization of the A-type of latent hamster virus.


Antibodies in chickens against avian leukosis virus group specific antigen.


Relationship between humoral antibodies and the failure to develop progressive tumors in cats injected with feline sarcoma virus (FSV).


Detection of the feline leukemia virus and other mammalian oncornaviruses by immunofluorescence.


Studies of human cells infected by leukemia viruses.


EBV antibody in monkeys and apes.

EBV antibody in monkeys and apes.


Antibodies to Epstein-Barr virus associated antigens in Burkitt's lymphoma.


Avian virus-induced leukemia-a model of cell differentiation.


Feline leukaemia virus infection--the spectrum of associated disease and its relevance to the pathogenesis and immunology of leukaemia.


Cell kinetics of virally-induced leukemias.


Guanine metabolism in preimplantation mouse embryo.


Interaction of Sendai virus and rabbit sperm: transmission and scanning electron microscopy.


Hormonal effects of estrogen-protein conjugates on rat uterus.


Therapy strategies, personality orientation and recovery from alcoholism.


Storage and activity of prostaglandin-like substance in body tissues of rats fed high fat diets.


Family planning: hospitals should expand services.


Bone case of the month.

Bone case of the month.


Thin fat people.

Thin fat people.


Distribution of women physicians, 1971.

Distribution of women physicians, 1971.


Do oral contraceptives need to be interrupted in order to treat vaginal candidiasis?


Improved Gram's stain for buffy coat smears.


Levels of nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide in facultative bacteria and the effect of oxygen. Nicotinamide adenine dinucleotide (NAD) and reduced NAD (NADH) levels have been measured in bacterial cultures. The cofactors were assayed by using the very sensitive cycling assay described previously by Cartier. Control experiments showed that the level of total NAD(H) falls during harvesting, and so samples were taken quickly from growing cultures and extracted immediately without separating the cells from the medium. Total NAD(H) ranged from 4.0 to 11.7 mumoles/g of dry cells for three facultative organisms, Klebsiella aerogenes, Escherichia coli, and Staphylococcus albus. NADH was remarkably constant in these bacteria; only one out of ten series of determinations was outside the range 1.4 to 1.9 mumoles/g of dry cells. NAD(+) showed much greater variation. An anaerobe (Clostridium welchii) had significantly more total NAD(H) whereas an aerobe Pseudomonas aeruginosa had about as much NAD(H) as the facultative organisms. NAD and NADH were measured during growth: once more NADH was much more constant than NAD. During change-over between aerobiosis and anaerobiosis, NADH showed a temporary increase but then returned to a constant level, whereas NAD changed from high aerobically to low anaerobically. These results are discussed in terms of the control mechanisms that may be involved.


Regulation of myo-inositol catabolism in Aerobacter aerogenes. A mutant of Aerobacter aerogenes produces constitutively the series of enzymes that mediates the degradation of myo-inositol and which in the wildtype strain is inducible. When grown on l-histidine, the mutant forms the enzymes at a level approximately three times as high as that seen in the induced wild type. The enzymes appear to be coordinately regulated and are sensitive to catabolite repression. Unless repressed, the synthesis of these enzymes markedly retards the growth of the mutant.


Kinetic properties of phosphotransacetylase from Veillonella alcalescens. The phosphotransacetylase of Veillonella alcalescens catalyzes a reversible reaction with Michaelis-Menten kinetics for all substrates. The rate of the reverse reaction (the synthesis of acetyl coenzyme A from acetyl phosphate) was 6.5 times greater than the rate of the forward reaction (the synthesis of acetyl phosphate from acetyl coenzyme A). The apparent K(m) values determined for the forward reaction were 8.6 x 10(-6)m for acetyl coenzyme A and 9.3 x 10(-3)m for phosphate. In the reverse reaction, the K(m) values were 3.3 x 10(-4)m for coenzyme A and 5.9 x 10(-4)m for acetyl phosphate. The results of an analysis of the inhibition by end products in the forward and reverse directions were compatible with a random bi- bi- mechanism. The enzyme was inhibited by adenosine triphosphate and adenosine diphosphate but was not affected by reduced nicotinamide adenine dinucleotide or pyruvate. The inhibition by adenosine triphosphate was noncompetitive with respect to acetyl phosphate and competitive with respect to coenzyme A. MgCl(2) reversed the inhibition by adenosine triphosphate or adenosine diphosphate. The role of Mg(2+) and adenylates in the regulation of phosphotranscetylase activity is discussed.


De novo biosynthesis of nicotinamide adenine dinucleotide in Escherichia coli: excretion of quinolinic acid by mutants lacking quinolinate phosphoribosyl transferase. The excretion of quinolinic acid was studied in growing and resting cells of Escherichia coli K-12 nadC(13). Under optimal conditions, this organism could synthesize quinolinic acid in several-fold excess of the amount which would be required for normal growth. The excretion of quinolinic acid was controlled by the concentration of nicotinamide adenine dinucleotide (NAD) precursors available to the organism either during growth or during incubation in dense cell suspensions. These observations suggest that biosynthesis of NAD de novo is regulated by both repression and feedback inhibition. Analogues of niacin which inhibit bacterial growth also inhibited and repressed the synthesis (excretion) of quinolinic acid. The pH optimum for quinolinic acid excretion agreed favorably with the optimum observed for its synthesis in vitro. The rate of quinolinic acid excretion was strongly influenced by the concentration of ribose or glycerol in the medium.


The pathogenicity of Entamoeba histolytica: is heat stress a factor?


The nature of the establishment barrier for Trypanosoma brucei in the gut of Glossina pallidipes.


Letter: Assay of serum enzymes in trypanosomal infection: interference by pyruvate.


The use of isocyanides for the immobilization of biological molecules.


The effects of nucleotides on the alpha-glucosidase formation in baker's yeast protoplasts.


Control of in vivo luminescence in psychrophilic marine Photobacterium.


Kinetic studies of the acylation of pig muscle D-glyceraldehyde 3-phosphate dehydrogenase by 1,3-diphosphoglycerate and of proton uptake and release in the overall enzyme mechanism. In the presence of NAD(+) the acylation by 1,3-diphosphoglycerate of the four active sites of pig muscle d-glyceraldehyde 3-phosphate dehydrogenase can be monitored at 365nm by the disappearance of the absorption band present in the binary complex of NAD(+) and the enzyme. A non-specific salt effect decreased the acylation rate 25-fold when the ionic strength was increased from 0.10 to 1.0. This caused acylation to be the rate-limiting process in the enzyme-catalysed reductive dephosphorylation of 1,3-diphosphoglycerate at high ionic strength at pH8. The salt effect permitted investigation of the acylation over a wide range of conditions. Variation of pH from 5.4 to 8.6 produced at most a two-fold change in the acylation rate. One proton was taken up per site acylated at pH8.0. By using a chromophoric H(+) indicator the rate of proton uptake could be monitored during the acylation and was also almost invariant in the pH range 5.5-8.5. Transient kinetic studies of the overall enzyme-catalysed reaction indicated that acylation was the process involving proton uptake at pH8.0. The enzyme mechanism is discussed in the light of these results.


A comparison of the physical and chemical properties of four cytochromes c from Azotobacter vinelandii. 1. A modified method for the separation and purification of four cytochromes c from Azotobacter vinelandii is described. Two new cytochromes c have been purified and are designated cytochromes c(551) and c(555). 2. Additional evidence is presented to establish the dihaem nature of cytochrome c(4). Ultracentrifugation data indicated similar molecular weights for the native and the denatured protein. Cleavage with CNBr yielded seven peptides; the amino acid compositions of the purified peptides were determined. Only one haem peptide was recovered. 3. Cytochromes c(551) and c(555) were characterized as acidic proteins of molecular weights about 12000. The spectral properties, isoelectric points, ;maps' of peptides from CNBr cleavage and amino acid compositions were determined for these two proteins. 4. The spectral properties, isoelectric points, molecular weights, CNBr peptide ;maps', amino acid compositions, relative oxidation-reduction potentials and e.p.r. (electron-paramagnetic-resonance) spectra of the four cytochromes c were compared. Cytochrome c(4) and cytochrome c(551) appear to be distinct proteins. The distinction between cytochromes c(5) and c(555) was not as clear, and our data are inadequate to establish firmly that they are distinct proteins. 5. The dihaem nature of cytochrome c(4) is evident in its e.p.r. spectrum. The e.p.r. spectra are similar to the spectra of mammalian cytochromes c.


Affinity chromatography of nicotinamide-adenine dinucleotide-linked dehydrogenases on immobilized derivatives of the dinucleotide. 1. Three established methods for immobilization of ligands through primary amino groups promoted little or no attachment of NAD(+) through the 6-amino group of the adenine residue. Two of these methods (coupling to CNBr-activated agarose and to carbodi-imide-activated carboxylated agarose derivatives) resulted instead in attachment predominantly through the ribosyl residues. Other immobilized derivatives were prepared by azolinkage of NAD(+) (probably through the 8 position of the adenine residue) to a number of different spacer-arm-agarose derivatives. 2. The effectiveness of these derivatives in the affinity chromatography of a variety of NAD-linked dehydrogenases was investigated, applying rigorous criteria to distinguish general or non-specific adsorption effects from truly NAD-specific affinity (bio-affinity). The ribosyl-attached NAD(+) derivatives displayed negligible bio-affinity for any of the NAD-linked dehydrogenases tested. The most effective azo-linked derivative displayed strong bio-affinity for glycer-aldehyde 3-phosphate dehydrogenase, weaker bio-affinity for lactate dehydrogenase and none at all for malate dehydrogenase, although these three enzymes have very similar affinities for soluble NAD(+). Alcohol dehydrogenase and xanthine dehydrogenase were subject to such strong non-specific interactions with the hydrocarbon spacer-arm assembly that any specific affinity was completely eclipsed. 3. It is concluded that, in practice, the general effectiveness of a general ligand may be considerably distorted and attenuated by the nature of the immobilization linkage. However, this attenuation can result in an increase in specific effectiveness, allowing dehydrogenases to be separated from one another in a manner unlikely to be feasible if the general effectiveness of the ligand remained intact. 4. The bio-affinity of the various derivatives for lactate dehydrogenase is correlated with the known structure of the NAD(+)-binding site of this enzyme. Problems associated with the use of immobilized derivatives for enzyme binding and mechanistic studies are briefly discussed.


The amino acid sequence of cytochrome c' from Alcaligenes sp. N.C.I.B. 11015. The amino acid sequence of the cytochrome c' from Alcaligenes sp. N.C.I.B. 11015 (Iwasaki's ;Pseudomonas denitrificans') has been determined. This organism is the only non-photosynthetic bacterium in which the protein has been found. The protein consists of a single polypeptide chain of 127 residues, with a single haem covalently attached to two cysteines. Unlike normal cytochromes c, the haem attachment site is very close to the C-terminus. The amino acid sequence around the haem attachment site is very similar to that of Chromatium vinosum D cytochrome c'. Detailed evidence for the amino acid sequence of the protein has been deposited as Supplementary Publication SUP 50022 at the British Library (Lending Division), (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1973) 131, 5.


The b-type cytochromes of bovine heart mitochondria: absorption spectra, enzymatic properties, and distribution in the electron transfer complexes.


Effects of oxandrolone on plasma lipoproteins and the intravenous fat tolerance in man.


Cytochrome c involved in the reductive decomposition of organic mercurials. Purification of cytochrome c-I from mercury-resistant Pseudomonas and reactivity of cytochromes c from various kinds of bacteria.


Letter: IIB or not IIB? Classification of hyperlipoproteinaemias.


Purification and properties of a mouse liver plasma-membrane glycoprotein hydrolysing nucleotide pyrophosphate and phosphodiester bonds. 1. A mouse liver plasma-membrane preparation was solubilized in an N-dodecylsarcosinate-Tris buffer, pH7.8, and the proteins and glycoproteins were separated by a rate-zonal centrifugation in sucrose-detergent gradients. 2. A peak of alkaline phosphodiesterase activity which sedimented ahead of the 5'-nucleotidase peak was associated with a major glycoprotein component of the plasma membrane. 3. The phosphodiesterase activity was then purified further by gel filtration and gave a single glycoprotein band after electrophoresis on polyacrylamide gels. The apparent molecular weight of the polypeptide at pH7.4 and 8.9 was 128000-130000 and was independent of the polyacrylamide concentration. Electrophoresis in gels containing deoxycholate showed that the protein band was coincident with phosphodiesterase activity. 4. After two-dimensional immunoelectrophoresis, with agarose containing rabbit anti-(mouse plasma-membrane) antiserum as second dimension, the enzyme showed one component which was also coincident with the phosphodiesterase activity. 5. An amino acid composition of the glycoprotein is presented. Carbohydrate analysis indicated the presence of glucosamine, neutral sugars and sialic acid. 6. The enzyme was also a nucleotide pyrophosphatase, as shown by a similar enrichment during purification of activity towards ATP, NAD(+), UDP-galactose and UDP-N-acetylglucosamine. The phosphodiesterase activity, measured by using dTMP p-nitrophenyl ester as substrate, was competitively inhibited by nucleotide pyrophosphate substrates. The enzyme showed little or no activity towards RNA, cyclic AMP, AMP, ADP and glycerylphosphorylcholine. 7. The significance of this enzyme activity in the plasma membrane is discussed.


Preparation of tissue fluid of the vessel wall and determination of its lipoproteins.


Shifts of bacteriochlorophyll and carotenoid absorption bands linked to cytochrome c-555 photooxidation in Chromatium.


On the passage-restricting role of acid mucopolysaccharides in the endothelium of pig aortas.


Immunochemical characterization of proteins from mouse liver plasma membranes. 1. Antiserum was prepared in rabbits against a purified mouse liver plasma-membrane fraction. 2. The antiserum was made to react with an (125)I-labelled alkaline-EDTA extract of the plasma membranes, and the immunoprecipitate analysed by polyacrylamide-gel electrophoresis. Seven proteins were immunoprecipitated and a single glycoprotein present in the alkaline-EDTA-soluble fraction was found to be a major component. 3. The alkaline-EDTA-soluble fraction was analysed by two-dimensional immunoelectrophoresis and this procedure indicated the presence of six antigenic components. 4. The plasma membranes were also extracted with 1% deoxycholate-1% Triton X-100; 50% of the protein, 80% of the alkaline phosphodiesterase activity and 30% of the 5'-nucleotidase activity were solubilized. 5. Two-dimensional immunoelectrophoresis of the deoxycholate-Triton X-100 extract indicated the presence of six antigens. 6. The relative distribution of the six antigens among the fractions obtained during the extraction procedure was examined immunoelectrophoretically to provide information on their disposition within the membrane.


Absorption changes of carotenoids and bacteriochlorophyll in energized chromatophores of Rhodospirillum rubrum.


Electron paramagnetic resonance signal II in spinach chloroplasts. I. Kinetic analysis for untreated chloroplasts.


Electron paramagnetic resonance signal II in spinach chloroplasts. II. Alternative spectral forms and inhibitor effects on kinetics of signal II in flashing light.


The back reaction in the primary electron transfer couple of photosystem II of photosynthesis.


C-550 in photosystem II subchloroplast particles.


Inorganic pyrophosphatase and photosynthesis by isolated chloroplasts. II. The controlling influence of orthophosphate.


Inorganic pyrophospatase and photosynthesis by isolated chloroplasts. I. Characterisation of chloroplast pyrophosphatase and its relation to the response to exogenous pyrophosphate.


Glia of myelination and fat deposit during early myelogenesis.


Effects of caffeine on radiation-induced phenomena associated with cell-cycle traverse of mammalian cells. Caffeine induced a state of G(1) arrest when added to an exponentially growing culture of Chinese hamster cells (line CHO). In addition to its effect on cell-cycle traverse, caffeine ameliorated a number of the responses of cells to ionizing radiation. The duration of the division delay period following X-irradiation of caffeine-treated cells was reduced, and the magnitude of reduction was dependent on caffeine concentration. Cells irradiated during the DNA synthetic phase in the presence of caffeine were delayed less in their exit from S, measured autoradiographically, and the radiation-induced reduction of radioactive thymidine incorporation into DNA was lessened. Cells synchronized by isoleucine deprivation, while being generally less sensitive to the effects of ionizing radiation than mitotically synchronized cells, were equally responsive to the effects of caffeine. The X-ray-induced reduction of phosphorylation of lysine-rich histone F1 was less in caffeine-treated cells than in untreated cells. Finally, survival after irradiation was only slightly reduced in caffeine-treated cells. A possible role of cyclic AMP in cell-cycle traverse of irradiated cells is discussed.


The metabolic and hemodynamic effects of prolonged bed rest in normal subjects.


An extralemniscal projection to the centrum medianum and pulvinar of the thalamus.


Estrogen and progestogen binders in human and rat mammary carcinoma.


Recovery of the genome of murine sarcoma virus (MSV) after infection of cells with nuclear DNA from MSV transformed non-virus producing cells.


The relationship between growth rate, labelling index and histological type of human solid tumours.


Postheparin plasma lipase in human subjects. Studies with chylomicrons, very-low density lipoproteins and a fat emulsion.


Fine structural studies in myasthenia gravis. I. Muscle fibre lesions.


A histochemical study of enzymes of carbohydrate metabolism in the rat metanephros.


Ultrastructural changes in the rat neurohypophysis in hyper- and hypofunction of adrenal glands.


Ultrastructural changes in nucleus supraopticus and paraventricularis of rat hypothalamus in hyper- and hypofunction of the adrenals.


Cytochemical and ultrastructural features of simian virus 40 transformed macrophages.


Ultrastructural changes of the small intestine in rats during neomycin induced enteropathy.


Studies on cytoplasmic tubular inclusions in papilloma and other epithelial tumours.


Ultrastructure and respiration of isolated heart cells.


The tumor-promoter phorbol ester (12-O-tetradecanoyl-phorbol-13-acetate), a potent aggregating agent for blood platelets. The phorbol ester 12-0-tetradecanoyl-phorbol-13-acetate, a potent tumor-promoting agent, caused irreversible platelet aggregation when more than 0.02 microM was stirred with human citrated or heparinized platelet-rich plasma (PRP). With washed platelets, 1 nM was effective. The alcohol phorbol, which has little tumor-promoting activity, failed to cause platelet aggregation. With all but low concentrations of phorbol ester, aggregation was succeeded by a rapid phase. The latter was prevented or reduced by enzymes which destroy ADP and by aspirin, was associated with a change in platelet shape, and was presumably due to released ADP. At higher concentrations, only a rapid phase was seen, and these inhibitors were not effective. Low concentrations did not aggregate platelets in PRP containing sufficient EDTA or EGTA to chelate ionized calcium or in PRP from thrombasthenic patients; higher concentrations caused slight aggregation. Both the primary, non-ADP-dependent aggregation and the rapid ADP-dependent aggregation were markedly inhibited by substances which increase cyclic AMP, metabolic inhibitors, and the sulfhydryl inhibitor N-ethylmaleimide. Phorbol ester reduced platelet cyclic AMP only when it had been previously elevated by prostaglandin E(1). 1 microM did not release beta-glucuronidase, lactic dehydrogenase, or inflammatory material from platelets in 4-5 min despite marked aggregation, but liberated all three in 30 min. The possibility is discussed that low phorbol ester concentrations cause primary aggregation by a direct action on platelet actomyosin.


Effect of cytochalasin B and colchicine on alpha-amylase release from rat parotid tissue slices.


The effect of cytochalasin B on pigment dispersion and aggregation in perfused Xenopus laevis tailfin melanophores.


Alkaline phosphatase activity and the regulation of growth in transformed mammalian cells.


The localization of enzymatic activities involved in uridine nucleotides reactions in human erythrocytes.


An improved method for isolating alpha granules and mitochondria from human platelets.


Erythrocyte adenine phosphoribosyltransferase in the Lesch-Nyhan syndrome.


Nucleoside diphosphate kinase at the cell surface of neoplastic human cells in culture.


Permeability changes during cell fusion.


Letter: Does perchlorate enhance brain lesion visibility?


Tumor scanning with 169Yb-citrate.

Tumor scanning with 169Yb-citrate.


Acetylcholinesterase localization in the giant nerve fiber of the squid.


The study of iodized oil emulsions for indirect lymphography.


Electron microscopic studies of plant tissue cultures infected with the aster yellows disease.


Inhibition of Herpes simplex virus replication by camptothecin.


Inhibition of Herpes simplex virus replication by cordycepin.


Swine vesicular disease.

Swine vesicular disease.


Organ culture of bovine teat skin and its application to the study of herpes mammillitis and pseudocowpox infections.


The influence of relative humidity on the stability of foot-and-mouth disease virus in aerosols from milk and faecal slurry.


The influence of haemoglobin on the fibrinolytic enzyme system.


The antiketogenic action of an anabolic steroid administered to ketotic cows.


The effects of antiinflammatory drugs on myeloperoxidase mediated iodination in human granulocytes.


Pharmacology of pressor agents on the systemic circulation.


The in vivo regulation of the progesterone "receptor" in guinea pig uterus: dependence on estrogen and progesterone.


Ultrastructure of Blastocystis hominis.

Ultrastructure of Blastocystis hominis.


The effects of niamid and reserpine on the nerve endings of the pineal gland.


The lymphatic capillaries of the middle ear mucosa in the guinea pig. Ultrastructure and permeability.


Persistent viral hepatitis.

Persistent viral hepatitis.


Fibrous histiocytomas of the orbit.

Fibrous histiocytomas of the orbit.


Prevalence of antibodies to herpesvirus types 1 and 2, arteritis and infectious anemia viral antigens in equine serum.


Ultrastructure of the synovial membrane of lambs affected with chlamydial polyarthritis.


Ganglionic and adrenergic effects of 2-phenylquinoline methiodide analogues.


Pharmacological influences on carbon dioxide-induced amnesia.


Cardiovascular effects of Cassella 7657 and its interaction with adenosine, NAD and aminophylline in dogs.


Fetal consequences of maternal viral infections in pregnancy.


Selective, near-total, irreversible inactivation of peripheral pseudocholinesterase and acetylcholinesterase in cats in vivo.


Effect of actinomycin D on uptake and incorporation of thymidine and hypoxanthine into the acid-soluble and acid-insoluble fractions of rat hepatoma cells grown in culture.


Effects of pyrazole, 4-bromopyrazole and 4-methylpyrazole on mitochondrial function.


The role of hemodynamic measurements in portosystemic shunt surgery.


Carbon tetrachloride induced liver alterations in rats pretreated with N,N'-diphenyl-p-phenylenediamine.


Effects of dichlorodiphenyltrichloroethane (DDT) analogs and polychlorinated biphenyl (PCB) mixtures on 17beta-(3H)estradiol binding to rat uterine receptor.


Protein iodination with solid state lactoperoxidase.


Immunospecificity of nonhistone proteins in chromatin.


Sequence composition of rat nuclear deoxyribonucleic acid and high molecular weight nuclear ribonucleic acid.


Calcium-induced phase separations in phosphatidylserine--phosphatidylcholine membranes.


Activity of liver alcohol dehydrogenase with various substituents on the amino groups.


Sequence composition of rat ascites chromosomal ribonucleic acid.


Inhibition of acetoacetate decarboxylase by ketophosphonates. Structural and dynamic probes of the active site.


Lymphocytosis induced in mice by supernatant fluids of Bordetella pertussis cultures: a histopathological study.


Interactions of normal and neoplastic fibroblasts with the substratum.


Dual-precipitation method for lipoprotein quantitation: improvement by membrane ultrafiltration of lipemic samples.


Time lapse studies on the motility of fibroblasts in tissue culture.


Letter: The ill-wind in direct analysis of plasma.


Progress report on long-term oestogen therapy.


The effect of acute or chronic ether stress on plasma ACTH concentration in the rat.


Effects of hCG, asialo-hCG and the subunits of hCG upon luteinization of monkey granulosa cell cultures.


ACTH effects on aldosterone production and mitochondrial ultrastructure in adrenal gland cultures.


Synergism among growth hormone, ACTH, cortisol and dexamethasone in the hormonal induction of diabetes in rats and the diabetogenic effect of tolbutamide.


The metabolism of cholesterol esters in rat adrenal cell suspensions.


Adrenal progesteerone: factors controlling its secretion.


Effect of dibutyryl cyclic AMP injected into third ventricle on the thyroid 131-I-iodide accumulation.


Extra-lysosomal localization of acid phosphatase in the spinal cord of the rat.


Somatotopic studies on cerebellar fastigial cells.


Evidence for GABA as the synaptic transmitter of the inhibitory vestibulo-ocular pathway.


Excitatory and inhibitory responses of neurones of the cerebellar fastigial nucleus.


The pathways responsible for excitation and inhibition of fastigial neurones.


Metabolism and isoenzyme alterations in experimental hepatomas.


The suppression of AD-3B mutants by supersuppressors in Neurospora crassa. The action of eight suppersuppressors has been tested on 76 ad-3B mutants which were induced by base-pair transition mutagens. Thirteen mutants were found to be suppressible by at least one ssu gene. Most of the suppressible mutants were found to belong to a class in which one would expect to find nonsense mutants (polar complementing or noncomplementing classes, with AT at the mutant site). However, suppression was observed in other classes, including those containing presumed missense mutants. The specificity of the suppressors and the criteria for molecular classification of the mutants are discussed.


Mosaic analysis of lethal mutations in Drosophila. The effects of various lethal mutations were studied when they were present in the hemizygous state as sectors in genetic mosaics (gynandromorphs) of Drosophila melanogaster. Sixty percent of the lethals studied can survive to adulthood in combination with normal tissue in mosaics, whereas the remainder cause the death of such mosaic animals. Some of the gynandromorph-viable lethals appeared to be locally active, in that they allowed the survival of only those mosaics in which the male tissue was present in the abdomen. The regions affected by these lethals were identified more precisely by analysis of the tissue distributions in the surviving gynandromorphs.


Dental erosion: progress of erosion measured clinically after various fluoride applications.


Hepatic cell carcinoma in a dog.

Hepatic cell carcinoma in a dog.


Comparative electrophoretic studies of foot-and-mouth disease virus proteins.


An interfering component of rabies virus which contains RNA.


A somatic cell approach to the genetic analysis of the laboratory mouse.


Electron microscopy and infectivity studies of the action of dimethylsulphoxide on two strains of tobacco mosaic virus.


Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses.


Refractory state of cells to interferon induction.


Heterogeneity of simian virus 40 populations: virus particles containing one or two virus DNA molecules?


Controlled trial of combinations of hydroxocobalamin-cystine and riboflavine-cystine, in Nigerian ataxic neuropathy. Chronic cyanide intoxication of dietary origin and riboflavine deficiency are believed to be major aetiological factors in Nigerian tropical ataxic neuropathy. The results are presented of a double-blind controlled therapeutic trial of combinations of large doses of hydroxocobalamin and cystine as cyanide binding agents together with riboflavine or placebos in Nigerian patients suffering from the tropical ataxic neuropathy. No clinical benefit was demonstrable with any of the treatments.


Disturbances in the voluntary recruitment order of anterior tibial motor units in spastic paraparesis upon fatigue. The recruitment order of motor units in the tibialis anterior muscle upon fatigue of tonic voluntary contraction was studied in 20 patients with severe spastic paraparesis. An electromyographic technique for secure identification of single motor units was used. Before fatigue the recruitment order is stable and low-frequency units are recruited before high-frequency units; this recruitment pattern agrees with that in normal voluntary activity. When fatigue appears, however, the recruitment order becomes indefinite and high-frequency units can be recruited before low-frequency units; this recruitment pattern agrees with that in normal phasic voluntary activity. Finally, all voluntary activation power disappears, even in the case of units which have never been active. Contraction ability and original recruitment order are restored upon rest but also upon tonic reflex support of the voluntary drive. Whether the fatigue reaction may be due to insufficient gamma motoneurone innervation and whether it is related to spasticity are discussed. The practical physiotherapeutic implications are reviewed.


Alterations in the surface glycoproteins of chicken erythrocytes following transformation with erythroblastosis strain R virus.


Motor unit estimations in small muscles of the hand. A quantal method has been employed to determine the numbers of functioning motor units in small muscles of the hand. In healthy subjects below the age of 60 years the mean number of hypothenar motor units was 380 ± 79 and the mean number of thenar units innervated by the median nerve was 340 ± 87. In older subjects there was a progressive reduction in the numbers of functioning units in both muscle groups; in some of these subjects the population of motor units in the extensor digitorum brevis was also estimated for comparison. A study was also performed on patients diagnosed as having generalized denervating diseases or with lesions of peripheral nerves and cervical roots; in every instance the number of functioning motor units was markedly reduced.


Mixed or immune complex cryoglobulinaemia and neuropathy. Three patients with peripheral neuropathy and mixed or immune complex cryoglobulinaemia are reported. The significance of mixed cryoglobulinaemia and the pathogenesis of the peripheral neuropathy are discussed.


Hypertrophic mononeuropathy.

Hypertrophic mononeuropathy. A case of hypertrophic mononeuropathy is described in a 19-year-old female. The entity is very rare as only three previous cases have been reported. The light and electron-microscope appearances resemble those of progressive hypertrophic polyneuritis.


Response of Escherichia coli to ethyl methanesulfonate: influence of growth phase and repair ability on survival and mutagenesis.


Chemically induced mutation of L-5178Y mouse leukemia cells from asparagine-dependence to asparagine-independence.


Humoral transmission of sleep. 8. Effects of the "sleep factor delta" on cerebral, motor and visceral activities.


Theophylline potentiation of in vitro effects of ACTH and catecholamines in rabbit adipose tissue.


Effect of coal and quartz dust on ciliary activity in the respiratory tract.


Cross-linking of collagen. Hydrogen abstraction studies on model compounds.


Identifying the organism in vulvovaginal infections.


Reye's syndrome: isolation of parainfluenza virus. Report of three cases.


Senescence and death of the conidiogenous cell in Stemphylium botryosum Wallroth.


Cerebellar toxic effects from nitrofurantoin.


Infection in the severely traumatized patient.


Adenosine 3',5'-monophosphate in cerebrospinal fluid. Effect of drugs and neurologic disease.


Hexachlorophene neuropathy.

Hexachlorophene neuropathy.


A summary and evaluation of spin labels used as probes for biological membrane structure.


Membrane fusion.

Membrane fusion.


Frog lactate dehydrogenase: kinetics at physiological enzyme levels.


Bilirubin: a potent inhibitor of NAD+-linked isocitrate dehydrogenase.


One-electron transfer reactions in biochemical systems. VII. Two types of electron outlets in milk xanthine oxidase.


One-electron transfer reactions in biochemical systems. 8. Kinetic study of superoxide dismutase.


Conversion of glycogen synthetase D of rat liver to a form that resembles synthetase D of skeletal muscle.


On the nature of the acceleration of the methanesulfonylation of acetylcholinesterase by tetraethylammonium.


Purification and characterization of a protein kinase in Tetrahymena cilia.


Kinetics of the reaction of human renin with natural substrates and tetradecapeptide substrate.


Effect of free magnesium and salts on the inorganic pyrophosphatase purified from a slightly halophilic Vibrio alginolyticus.


A spin label investigation of some model membrane systems.


Mass spectra of trimethylsilyl derivatives of homogeneous cerebrosides (monoglycosylceramides).


Muscular dystrophy in childhood.

Muscular dystrophy in childhood.


Iron transport by phospholipids in a two phase system containing water and n-pentanol.


Effects of quantal unit latency on statistics of Poisson and binomial neurotransmitter release mechanisms.


Surgery of bilateral Wilms tumors: the role of renal transplantation.


Congenital solitary kidney: a review of 74 cases.


Plasma renin and aldosterone after renal transplantation.


Adenylate cyclase activity in rat submandibular gland during postnatal development.


Further characterization of mono and diglyceride lipases in rat tissues.


The effects of silica dust and alveolar macrophages on lung fibroblasts grown in vitro.


The interaction between Entamoeba histolytica and Schistosoma mansoni infections in mice.


Indirect immunofluorescent staining of trophozoites of Entamoeba histolytica.


Canine malignant mammary tumors. 3. Special types of carcinomas, malignant mixed tumors.


Permanent mixed-field polyagglutinability (PMFP). I. Serological observations.


A possible direct action of ACTH on nerve and muscle.


Permanent mixed-field polyagglutinability (PMFP). II. Hematological, biophysical and biochemical observations.


Action of cyclic AMP on pigment donation between mammalian melanocytes and keratinocytes.


Molecular biology of pigment cells. Molecular controls in mammalian pigmentation.


Effects of calcitonin on osteoclasts in vivo. An ultrastructural and histochemical study.


Ultrastructure and cell biology of pigment cells. Intracellular dynamics and the fine structure of melanocytes with special reference to the effects of MSH and cyclic AMP on microtubules and 10-nm filaments.


On the fine structure of the Octopus iris.


Association of renal allograft rejection with virus infections.


Fine structural studies of rat adrenal cortices following prostaglandin administration.


Muscle spindle distribution in the masticatory muscle of the squirrel monkey (Saimiri sciurea).


Arsenical polyneuropathy-a clinical study.


Progesterone binding in the immature rabbit and guinea pig uterus.


In vivo action of vinblastine on thyroid ultrastructure and hormone secretion.


Studies on cyclic nucleotides in the adrenal gland. IV. Effect of ACTH on cyclic nucleotides-dependent protein kinases in the adrenal gland.


Fine structure and innervation of the avian adrenal gland. V. Innervation of interrenal cells.


Effect of hypophysectomy and adrenalectomy on glucagon and insulin concentration.


Effect of ACTH and camp on human adrenocortical growth and function in vitro.


Androgen receptors in mouse kidney: a study of male, female and androgen-insensitive (tfm-y) mice.


Hormonal control of adrenal RNA polymerase activities.


Glycogen metabolism and its control by norepinephrine in cardiac Purkinje fibers.


Studies on the nature of the hypothalamic estradiol-concentrating mechanism in the male and female rat.


Oral contraceptives: ethinyl estradiol binds with higher affinity than mestranol to macromolecules from the sites of anti-fertility action.


In vitro arterial perfusion of the dog adrenal.


Inhibition of luteinization of transplanted rat ovarian follicles by polyphloretin phosphate.


Purification and immunochemical studies of the protein-bound J substance from cattle serum.


Studies on the effect of cyclic-AMP on the ingibition of lipolysis by insulin & some oral hypoglycaemic agents.


Carcinoembryonic antigen (CEA) in non digestive cancerous and normal tissues.


Estradiol receptor in rat testis.

Estradiol receptor in rat testis.


Viruses associated with clinical cases with special reference to poliomyelitis epidemic of 1971 in and around Chandigarh (Northern India).


In vivo effects of phytohaemoagglutinin on NAD-glycohydrolase activity and histological features of spleen, thymus and liver in the rat.


Chemotaxis of polymorphonuclear leukocytes in corneal inflammation: tissue injury in herpes simplex virus infection.


Effects of isoproterenol and cyclic AMP derivatives on cell division in cultured rat lenses.


Changes in the contents of cholesterol and phosphatases in the lungs of rats exposed to mica dust.


Multiple sclerosis: an overview, with some observations on the spinal fluid protein.


Procarbazine hydrochloride in the treatment of brain tumors. Phase 2 study.


A technique for relief of motor and sensory deficits occurring after anterior ulnar transposition. Technical note.


Chromosome breakage in Drosophila melanogaster induced by a monofunctional alkylating agent (EMS).


Investigation of the antifungal activity of the Nelex solution.


Letter: Cyclic AMP and corticosteroids.

Letter: Cyclic AMP and corticosteroids.


Letter: Possible neuropathy from methyl N-butyl ketone.


Letter: Abdominal pain in hyperlipoproteinemia.


Letter: EBV and American Burkitt's lymphoma.


Neurologic manifestations in patients with mixed cryoglobulinemia.


Extra-articular pigmented villonodular synovitis affecting bone. The role of angiography as an aid in its differentiation from similar bone-destroying conditions.


Spinal cord pathways involved in tourniquet stimulation of ACTH secretion.


Effects of cortical spreading depression on multiunit activity in the preoptic area and hypothalamus of the female rat.


Influence of 6-hydroxydopamine on hypothalamic control of prolactin and ACTH secretion in the teleost fish, Tilapia mossambica.


Physiological regulation of tissue-CRF.

Physiological regulation of tissue-CRF.


A selective medium for the isolation of Corynebacterium pyogenes.


ACTH release in rats after removal of the medial hypothalamus.


Detection of cyclic AMP in parotid saliva of normal individuals.


The effect of p,p'-DDT (Dicophane) and its main metabolites on the respiration of rat liver mitochondria.


Hypertension: a disease of abnormal circulatory control.


A catecholamine secreting retroperitoneal glomangioma. A case report.


The effect of photosensitizing dyes on the 3H-thymidine incorporation of cells grown in vitro.


Enhancement of senescence in low passage human embryonic lung cells by an agent extracted from phase 3 cells.


Stepwise accumulation of autoregulated enzyme activities during the cell cycle of the eucaryote Chlorella.


Myogenic cells derived from murine sarcoma induced mouse tumours: DNA synthesis and the effects of simian virus 40 infection.


Activation of nuclear chromatin and the release from contact-inhibition of 3T3 cells.


Maturation-stimulation effect of brain extract and dibutyryl cyclic AMP on dissociated embryonic brain cells in culture.


Citric acid-sonication method for the isolation of nucleoli.


Deoxyribonuclease in sea urchin embryos. Comparison of the activity present in developing embryos, in nuclei, and in mitochondria.


Differential sensitivity of antigen- and mitogen-stimulated human leukocytes to prolonged inhibition of potassium transport.


Nature of the specificity of alcohol coupling to L-alanine transport into isolated membrane vesicles of a marine pseudomonad. Ethanol stimulated the uptake of l-alanine into isolated membrane vesicles of a marine pseudomonad at a rate and to an extent comparable with that obtained with reduced nicotinamide adenine dinucleotide (NADH) or the artificial electron donor ascorbate-N, N, N', N'-tetramethyl-p-phenylenediamine (ascorbate-TMPD). Methanol and branched-chain alcohols had little or no capacity to energize transport. No quantitative relationship was found between the ability of a compound to induce oxygen uptake and to energize transport, since with ethanol initial rates of oxygen uptake were approximately 4% of that obtained with NADH or ascorbate-TMPD. Cytochrome analysis revealed that NADH and ethanol reduced cytochromes b and c, whereas ascorbate-TMPD coupled primarily at the level of cytochrome c. Approximately 25% of the cytochromes reduced by dithionite were reducible by ethanol. Ethanol reduction of both cytochromes b and c was prevented by 2-heptyl-4-hydroxyquinoline-N-oxide, p-chloromercuribenzoate, N-ethylmaleimide, and iodoacetate. The ethanol- and NADH-energized transport systems for l-alanine were subject to quantitatively similar inhibition by cyanide, 2-heptyl-4-hydroxyquinoline-N-oxide, 2, 4-dinitrophenol, and the sulfhydryl reagents p-chloromercuribenzoate, N-ethylmaleimide, and iodoacetate. In contrast, for ascorbate-TMPD-driven transport, only cyanide and 2, 4-dinitrophenol remained fully effective as inhibitors, p-chloromercuribenzoate was only half as effective, and the other compounds stimulated transport. Inhibition of ethanol oxidation strikingly paralleled the inhibition of ethanol-driven transport for each of the inhibitors, including 2, 4-dinitrophenol. Marked differences between inhibition of oxygen uptake and inhibition of transport were observed when NADH or ascorbate-TMPD were the electron donors. The data indicate that only a small proportion of the respiratory chain complexes in the membrane vesicles are involved in transport and these are efficiently coupled to ethanol oxidation. The results also suggest that when 2, 4-dinitrophenol inhibits transport it is not acting as an uncoupling agent.


Specific electron donor-energized transport of alpha-aminoisobutyric acid and K+ into intact cells of a marine pseudomonad. The transport of alpha-aminoisobutyric acid and K(+) into K(+)-depleted cells of a marine pseudomonad (ATCC 19855) was stimulated strongly by ethanol, reduced nicotinamide adenine dinucleotide (NADH), and ascorbate-reduced N, N, N', N'-tetramethyl-p-phenylenediamine. In the presence of the quinone inhibitor 2-heptyl-4-hydroxyquinoline-N-oxide, only ascorbate-reduced N, N, N', N'-tetramethyl-p-phenylenediamine was active. Primary and secondary, but not tertiary, alcohols from ethanol to n-amyl alcohol stimulated both alpha-aminoisobutyric acid and K(+) transport and were oxidized by the cells. Malate and succinate, which were oxidized rapidly by the cells, had little or no capacity to energize the transport of alpha-aminoisobutyric acid into K(+)-depleted cells but were partially effective in promoting K(+) uptake. Ethanol stimulated the transport of alpha-aminoisobutyric acid into K(+)-preloaded cells. The transport of both alpha-aminoisobutyric acid and K(+) was inhibited 20% by iodoacetate, 85% by N-ethylmaleimide, and 90 to 100% by both NaCN and p-chloromercuribenzoate. The addition of Na(3)Fe(CN)(6) permitted the ethanol-induced transport of alpha-aminoisobutyric acid into K(+)-preloaded cells in the presence of NaCN, but little or no uptake of alpha-aminoisobutyric acid or of K(+) into K(+)-depleted cells under the same conditions. The transport of alpha-aminoisobutyric acid into K(+)-depleted cells required both K(+) and an electron donor. The oxidation of NADH and ethanol by K(+)-depleted cells was stimulated strongly by K(+). Parallels between these studies and those with membrane vesicles show that results with membrane vesicles of the marine pseudomonad have physiological significance for the intact cells. The results support the conclusion that the energy for the active transport of both alpha-aminoisobutyric acid and K(+) into cells of this organism is provided by electron flow through a region of the respiratory chain lying between cytochrome c and O(2).


De novo purine synthesis in vegetative cells and myxospores of Myxococcus xanthus. This study was designed to determine whether vegetative cells and myxospores of Myxococcus xanthus were capable of classical de novo purine biosynthesis. To answer this question, vegetative and myxospore extracts of M. xanthus FBa were tested for their ability to synthesize the second de novo intermediate, 5'-phosphoribosylglycinamide, from beginning precursors either by way of phosphoribosyl-pyrophosphate amido transferase (EC 2.4.2.14) or ribose-5-phosphate amino transferase. Both the amido and amino transferase routes occurred in both types of extracts, and both enzymes appear to be present at about the same level (per milligram of protein) in vegetative cells, myxospores, and in a bacterial prototype, Salmonella typhimurium. The dose response of the vegetative and myxospore forms of both enzymes towards adenosine 5'-monophosphate (AMP) and guanosine 5'-monophosphate (GMP) suggests that the allosteric structure of both enzymes is changed little by sporulation. Both enzymes were inhibited to varying degrees by a variety of purine nucleotides besides AMP, GMP, and 3':5' cyclic AMP.


Fatty acid degradation in Escherichia coli: requirement of cyclic adenosine monophosphate and cyclic adenosine monophosphate receptor protein for enzyme synthesis. The strong repression of inducible synthesis of the enzymes of fatty acid degradation by glucose can be partially relieved by the addition of cyclic adenosine 3',5' monophosphate (cyclic AMP) to the growth medium. This reversal of the glucose effect by cyclic AMP is not observed in a mutant (K29) that is unable to grow on fatty acids as sole carbon source and that was found to synthesize low levels of several enzymes specified by the fad regulon. In a revertant selected for the ability to grow on oleate these effects are concomitantly relieved. By both genetic (co-transduction of the mutation with the strA locus) and biochemical experiments (an extract of the mutant strain does not show the cyclic AMP-dependent stimulation of the deoxyribonucleic acid-directed in vitro synthesis of the enzymes of the gal operon), it is demonstrated that the mutant lacks functional cyclic AMP receptor protein (CR protein). It is concluded that, like many other inducible enzyme systems, expression of the enzymes of the fad system requires cyclic AMP and the CR protein.


Isolation and characterization of temperature-sensitive mutants of host-dependent Bdellovibrio bacteriovorus 109D. A variety of temperature-sensitive mutants of host-dependent Bdellovibrio bacteriovorus 109D were selected after ethyl methane sulfonate mutagenesis. Mutants that demonstrated plaque-forming ability reversion frequencies of 10(-8) to 10(-9) were chosen for further study. Representatives of these mutants were then characterized by phase-contrast and electron microscopy, temperature-shifted one-step growth experiments, attachment kinetics, and macromolecular capabilities. Representative mutants demonstrate various types of blockage corresponding to the previously described morphological stages of Bdellovibrio predatious life cycle, i.e., attachment blockage (109D153), penetration blockage (109D3 and 109D48), and blockage of intracellular growth (109D4 and 109D152). The time of release from temperature repression for the mutant classes was found to correspond to the apparent morphological stage of blockage via temperature-shifted, one-step growth experiments. Mutants characterized as exhibiting blockage in the penetration or intracellular stages of the infection cycle exhibited, at the permissive and nonpermissive temperatures, kinetics of attachment to Escherichia coli WP2 similar to those of the wild type. One mutant, 109D153, exhibited depressed attachment at the restrictive temperature even though the Bdellovibrio cells were motile. The extent of 38.5 C attachment of 109D153 to E. coli is at the same level as that of wild-type 109D to Bacillus subtilis, a gram-positive, non-host organism. Subsequent detachments were revealed in the wild-type 109D-B. subtilis or mutant 109D153-Escherichia coli (38.5 C) cultures. These studies reveal a biphasic attachment phenomenon in the early interaction of Bdellovibrio with its host. It appears that, at the restrictive temperature, 109D153 is capable only of the initial, nonspecific type of attachment.


Isolation of a regulatory mutant of fructose-1,6-diphosphatase in Saccharomyces carlsbergensis. By selecting for growth on glycerol, but absence of growth on glucose, a mutant of Saccharomyces carlsbergensis was isolated which does not grow on glucose, fructose, mannose, or sucrose, which shows long-term adaptation to maltose, but which can grow normally on galactose, ethanol, or glycerol. In the mutant, fructose diphosphatase is not inactivated after the addition of glucose, fructose or mannose to the medium, resulting in the simultaneous presence of fructose diphosphatase and phosphofructokinase activity. Under these conditions, a cycle is probably catalyzed between fructose-6-phosphate and fructose-1,6-diphosphate, resulting in the net consumption of adenosine triphosphate and an immediate stop of protein synthesis.


Antisera to human cytomegaloviruses prepared in the guinea pig: specific immunofluorescence and complement fixation tests.


Reversion of frameshift mutations by mutator genes in Escherichia coli. The Escherichia coli mutator genes mutU4, mutS3, and mut-25 (a possible allele of mutL), previously known to induce transitional base changes, increased significantly the frequencies of reversion of lacZ frameshift mutations. mutT1, previously shown to induce only the transversion of adenine-thymine to cytosine-guanine, had no effect on the reversion of lacZ frameshift mutations. With mutator genes other than mutT1, small increases were found in the frequencies of reversion of trpA frameshift mutations.


Evidence for in vivo protection against murine-sarcoma virus-induced tumors by T lymphocytes from immune animals.


Regulation of tryptophan biosynthesis in Saccharomyces cerevisiae: mode of action of 5-methyl-tryptophan and 5-methyl-tryptophan-sensitive mutants. In a wild-type strain of Saccharomyces cerevisiae the tryptophan analogue dl-5-methyl-tryptophan (5MT) causes only a slight reduction of the growth rate. Uptake experiments indicate that the limited inhibition is partly due to low levels of 5MT inside the cell. On the other hand, this low concentration of 5MT leads to an increase in the activity of the tryptophan-biosynthetic enzymes. Evidence is presented that suggests that 5MT acts primarily through feedback inhibition of anthranilate synthase, the first enzyme of the pathway. A number of 5MT-sensitive mutants have been isolated, characterized, and assigned to one of the following three classes: class I, strains with altered activity and/or feedback sensitivity of anthranilate synthase; class II, strains with elevated uptake of 5MT; class III, mutants with altered regulation of the tryptophan-biosynthetic enzymes, which do not exhibit increases in activity in the presence of 5MT. This failure to exhibit increased enzyme activities in mutants of class III can also be observed after tryptophan starvation. Two mutants of class III show high sensitivity towards 3-amino-1,2,4-triazole. They can not exhibit derepression of some histidine- and arginine-biosynthetic enzymes under conditions that lead to an increase in these same enzymes in the wild-type strain.


Ruptured abdominal aortic aneurysm presenting with peripheral neuropathy.


In vitro detection of immune responses to MTV-induced mammary tumors: activity of spleen cell preparations from both MTV-free and MTV-infected mice.


Lymphocyte interferon production and transformation after Herpes simplex infections in humans.


Comparison of insulin and proinsulin storage in an islet adenoma and adjacent pancreas.


Perspectives on the renin-angiotensin-aldosterone system in hypertension.


Adrenocortical structure and function in rats bearing an adrenocorticotrophic hormone, growth hormone, and prolactin-secreting tumor.


Alcohol and cancer.

Alcohol and cancer.


Hypothalamic inputs: methods, and five examples.


Basic electrophysical mechanisms in neuroendocrinology.


Hypothalamic mechanisms controlling pituitary function.


Activation of lymphoid leucosis in chicks by irradiation.


Home care program cuts dollars and days.


The Baycrest experience: make your beds go further.


Modes of Epstein-Barr virus infection in human floating cell lines.


Further studies on antibodies to early antigens induced by Epstein-Barr virus in nasopharyngeal carcinoma patients.


Differential inhibition of histoformative aggregation of rat hepatoma cells in culture by concanavalin-A.


Tumor inhibitory effect of a new series of methanesulfonate of aminoglycols.


Assay of Epstein-Barr virus by immunofluorescence.


Heterogeneity of catalase in Morris hepatomas as revealed by isoelectric focusing and deae-cellulose column chromatography.


Brain tumors induced in adult monkeys by Schmidt-Ruppin strain of Rous sarcoma virus.


Separation of dl-2-(3-phenoxyphenyl)propionic acid (fenoprofen) from its intermediates by thin layer chromatography.


An isolation and cleanup procedure for low levels of organochlorine pesticide residues in fats and oils.


Bluetongue virus transmission with Culicoides variipennis via embryonating chicken eggs.


Physiology of neuromuscular transmission.


Hepatic scintoscans.

Hepatic scintoscans.


The work of the primary liver cancer research project: the value of liver function tests in the diagnosis of primary cancer of the liver in the Bantu.


The modern face of rheumatic fever.

The modern face of rheumatic fever.


Fibrinous pseudo-intimas within impermeable silastic-nylon velour. II. Development of "neointima".


Hemolytic activity of mica dust and its relation to polyvinylpyridine N-oxide.


Frey's syndrome: diagnosis and treatment.


Replication of the resident non-defective SV40 in transformed human cells superinfected with DNA from a temperature-sensitive SV40 mutant.


Relationship between cell-interaction and antiviral activity of polyriboinosinic acid-polyribocytidylic acid in different cell cultures.


Electron microscopic observations on the mechanisms of entry of simian foamy virus in HEp-2 cells.


Characterization of Herpes simplex virus types 1 and 2 adapted to growth at 25 degrees C.


Mass production of human interferon in diploid cells stimulated by poly-I:C.


A comparative study of Tanapox and Yaba viruses.


Interferon assay based on the inhibition of double-stranded reovirus RNA accumulation in mouse L cells.


Haemolysis by Sendai virus: involvement of a virus protein component.


Virocidal cleavage of disulphide bonds within structural proteins of Sendai virus.


Blocking of the virus-enhancing activity of 5-methyl-2-D-ribobenzimidazole (MRB).


Behavior of human cytomegaloviruses in cell cultures of bovine and simian origin.


Spontaneous disappearance of tumorigenicity in a polyoma virus-induced neoplasm carried in vitro.


Regional cyclic nucleotide phosphodiesterase activity in cat central nervous system: effects of benzodiazepines.


Neurogenic hypercholesterolemia: influence upon lipoproteins.


Reversible inhibition by hyaluronidase of the insulinotropic action of tolbutamide in isolated hamster pancreatic islets.


The binding of 131-I-HCG to mature follicles isolated from the rabbit ovary.


Tilorone: its selective effects on humoral and cell-mediated immunity.


Ontogenesis of circadian pituitary-adrenal periodicity in rats affected by neonatal treatment with ACTH.


A survey of cats and humans for prevalence of feline leukemia-sarcoma virus neutralizing serum antibodies.


Lack of correlation between initial vascular resistance and responses to vasoconstrictor stimuli in the perfused canine hindpaw.


Effect of heparin on the kinetics of guinea pig and human postheparin lipase activity.


Complement-fixing antibodies reactive with Epstein-Barr virus in sera of marmosets and prosimians.


Microneutralization test for detection of rhinovirus antibodies.


Lack of interaction between glucocorticoids and the kallikrein-kinin system.


Studies of cyclic AMP effect on release of unsaturated vitamin B 12 binding capacity from human blood cells.


Ocular illness in association with adenovirus type 3 infection. An epidemic of conjunctivitis and keratoconjunctivitis in Stockholm 1969.


Osteoblastic multiple myeloma.

Osteoblastic multiple myeloma.


Spironolactone inclusions in an aldosteronoma.


Angiographic evaluation of primary malignant hepatocellular tumors in children.


Cold-reactive antibodies in a case of congenital cytomegalovirus infection.


Phlebo-arterio-urography in the assessment of abdominal masses in children.


B-scan diagnostic ultrasound in the pediatric patient.


Prognostic factors in carcinoma of the stomach.


The anti-anaphylactic actions of disodium cromoglycate, theophylline, isoprenaline and prostaglandins.


Studies on the reduction of tetrazolium salts. I. The isolation and characterisation of a half-formazan intermediate produced during the reduction of neotetrazolium chloride.


Thin layer chromatography and in situ fluorometry for the quantitative determination of dimethoate and malathion in lettuce.


Microbiological methods. Collaborative study of the alpha-toxin method for estimating population levels of Clostridium perfringens in food.


Anaerobic synthesis of apocytochrome oxidase and assembly of the holoenzyme in yeast protoplasts.


Coproporphyrin 3, inhibitor of the synthesis of cytochrome oxidase in anaerobic yeast protoplasts.


Interaction of an active glycopeptide from chick skin collagen (alpha 1-CB5) with human platelets.


Glucocorticoid receptors in rat thymocytes. Interactions with the antiglucocorticoid cortexolone and mechanism of its action.


Spectroscopic studies on the conformation of cytochrome c and apocytochrome c.


Interaction of nucleotides with liver uridine diphosphate-glucose-4'-epimerase.


Effects of 25-hydroxyvitamin D3 on rat duodenum, jejunum, and ileum. Correlation of calcium active transport with tissue levels of vitamin D3 metabolites.


Studies on the regulation of gluconeogenesis in isolated rat liver cells by epinephrine and glucagon.


Protein and glycoprotein subunit composition of plasma membrane from chick embryo fibroblasts.


Characterization of protein kinases from rabbit reticulocytes.


The role of ribosomal proteins L7 and L-12 in polypeptide chain initiation in Escherichia coli.


Structural study of the blood group A active glycolipids of hog gastric mucosa.


Testicular adenosine 3':5'-monophosphate-dependent protein kinase. Regulation by follicle-stimulating hormone.


1 Alpha,25-dihydroxycholecalciferol receptors in intestine. I. Association of 1 alpha,25-dihydroxycholecalciferol with intestinal mucosa chromatin.


1 Alpha,25-dihydroxycholecalciferol receptors in intestine. II. Temperature-dependent transfer of the hormone to chromatin via a specific cytosol receptor.


Rabbit muscle phosphofructokinase. II. Product and dead end inhibition.


The regulation of lipogenesis by cyclic nucleotides in intact hepatocytes prepared by a simplified technique.


Thin-layer chromatography of various analgesics, antipyretics and anti-inflammatory agents.


Enzyme-catalyzed free radical reactions with nicotinamide adenine nucleotides. II. Lactate dehydrogenase-catalyzed oxidation of reduced nicotinamide adenine dinucleotide by superoxide radicals generated by xanthine oxidase.


Cerebral arterial spasm. 1. In vitro contractile activity of vasoactive agents on canine basilar and middle cerebral arteries.


Cellular action of antidiuretic hormone in nephrogenic diabetes insipidus.


Detection of Australia antigen (HBAg) in blood donors and hepatoma patients in Mozambique.


Effect of heterologous transplantation of H4-II-E rat hepatoma cells into the cheek pouch of hydrocortisone treated hamsters.


Group I inhibition in Ib excited ventral spinocerebellar tract neurones.


Effects of 6-OH-dopamine on the autonomic nerves of the rabbit myometrium.


Vascular and metabolic effects of theophylline, dibuturyl cyclic AMP and dibuturyl cyclic GMP in canine subcutaneous adipose tissue in situ.


Observations on closed tissue cultures of sympathetic ganglia of chick embryos in media buffered with N-tris-(hydroxymethyl) methyl-glycine or N-2-hydroxy-ethylpiperazine-N-2-ethanesulfonic acid.


A long term study on the ocular lesions in streptozotocin diabetic rats.


Stimulation of dendritogenesis in human melanocytes by dibutyryl adenosine 3', 5'-cyclic monophosphate in vitro.


Langerhans cells in cell culture. Survival and identification.


Influence of mitochondria on phospholipid synthesis in preparations from rat liver. 1. The addition of mitochondria to an incubation system containing the soluble and microsomal fractions of rat liver enhances severalfold the incorporation of each of ethanolamine, phosphorylethanolamine and CDP-ethanolamine into phosphatidylethanolamine. 2. In the presence of microsomal, mitochondrial and soluble fractions, CDP-ethanolamine exhibits the greatest initial rate of incorporation (approx. 6nmol/h per mg of protein), being slightly faster than that of phosphorylethanolamine (approx. 5nmol/h per mg of protein). Incorporation of ethanolamine proceeds very slowly for the first 20min and only after 30min gives rates approaching those of the other two precursors. 3. By using a substrate ;dilution' technique it was shown that in the reconstituted system the affinity of each of the enzymes for their respective substrates is very high: 10mum for ethanolamine, 25mum for phosphorylethanolamine and 5mum for CDP-ethanolamine. 4. Isolation of the mitochondrial and microsomal fractions from the medium after incubation together with phosphorylethanolamine showed that about 70% of the total radioactivity was present in the microsomal fraction and about 30% in the mitochondria after only 20min. Similar experiments with ethanolamine as precursor revealed that after 20min only about 15% of the total radioactivity was present in the mitochondria but that after 40min about 30% was present in this fraction. 5. Heating and phospholipase treatment of mitochondria, but not freeze-thawing, eliminated the stimulatory effect of mitochondria on phospholipid synthesis. 6. The reconstituted system exhibits an absolute requirement for Mg(2+) (2mm gave maximal rates) and is inhibited by very low concentrations of Ca(2+) (100mum-Ca(2+) produced half-maximal inhibition with 3mm-Mg(2+)). Further addition of Mg(2+) overcame the Ca(2+) inhibition, suggesting that the inhibitory effect is readily reversible. 7. The concept that modification of the Mg(2+)/Ca(2+) ratio is a means of controlling the rate of cellular phospholipid synthesis is introduced.


Phosphatidylinositol kinase. A component of the chromaffin-granule membrane. Phosphorylation of bovine chromaffin granules by ATP leads to the formation of diphosphoinositide in the granule membrane. Both phosphatidylinositol kinase and its substrate are components of this membrane, and triphosphoinositide is not formed under the conditions of the assay. The reaction is Mg(2+)-dependent and is stimulated by Mn(2+) and F(-) ions. The initial reaction is rapid, with a broad pH profile and a ;transition' temperature for its activation energy at 27 degrees C. The apparent K(m) for ATP is 5mum. ATP, N-ethylmaleimide, Cu(2+) ions and NaIO(4) are inhibitory. The phospholipids of chromaffin-granule membranes have been analysed: 6.8% of the lipid P is found in phosphatidylinositol, and only 2-3% in phosphatidylserine. Comparison of the rate of phosphorylation of intact and lysed granules suggests that the sites for phosphorylation are on the outer (cytoplasmic) surface of the granules, and diphosphoinositide may therefore make an important contribution to the charge of the chromaffin granule in vivo.


Plasma lipoproteins and the synthesis and turnover of plasma triglyceride in normal and genetically obese mice. 1. Lipoproteins in the plasma of mice were characterized by agarose-gel chromatography and polyacrylamide-gel electrophoresis: genetically obese (ob/ob) mice exhibited hyperlipoproteinaemia (compared with lean mice), largely owing to an increase in the concentration of cholesterol in high-density lipoprotein. Plasma concentrations of triglyceride and phospholipid were not markedly increased in genetically obese mice. 2. The formation of glycerolipids in liver and plasma was investigated with (14)C-labelled precursors. The synthesis of hepatic triglyceride and phospholipid from glucose or palmitate was enhanced in ob/ob mice, compared with lean mice. The rate of entry of triglyceride into plasma, calculated from the time-course of incorporation of (14)C from [(14)C]palmitate into plasma triglyceride, was increased in ob/ob mice (0.5mumol of fatty acid/min, compared with 0.2 in lean mice). 3. The removal from plasma of murine lipoprotein triglyceride-[(14)C]fatty acid was increased in ob/ob mice (half-time 2.2min, compared with 7.2min in lean mice). Similar results were obtained with an injected lipid emulsion (Intralipid). 4. From these measurements, estimates of the rates of turnover of plasma triglyceride in mice (fed on a mixed diet, female, 3 months old) are about 1.0mumol of fatty acid/min in ob/ob mice, and 0.25 in lean mice. 5. The major precursor of hepatic and plasma triglyceride in lean and ob/ob mice was calculated to be plasma free fatty acid. 6. These results are discussed, in connexion with the role of the liver in triglyceride metabolism in mice, especially in relation to genetic obesity.


Stimulation of ribonucleic acid polymerase activity in vitro by prostatic steroid-protein receptor complexes. A system has been developed which allows the stimulation in vitro of prostatic RNA polymerase by prostatic 5alpha-dihydrotestosterone-protein receptor complexes prepared from the tissues of castrated rats. The reconstitution in vitro of such a system necessitates the purification of several subcellular components. Two 5alpha-dihydrotestosterone-receptor complexes are located in the prostatic soluble supernatant fraction, separable by selective ammonium sulphate fractionation, and one complex can be isolated from the nuclear fraction. In the presence of all these complexes, stimulation of RNA polymerase in intact nuclei and nucleoli was observed. The complexes also increased the activity of the enzyme solubilized from whole nuclei. Greater stimulation of this system was noted in the presence of prostatic chromatin as template, as compared with that observed with calf thymus DNA or liver chromatin as template. The effects of the complexes on subnuclear forms of RNA polymerase, of nucleolar and extranucleolar origin, are also described. RNA polymerase solubilized from nucleoli is more susceptible to stimulation by the 5alpha-dihydrotestosterone-receptor complexes than is the ;nucleoplasmic' enzyme. Stimulation occurs less readily in the presence of Mn(2+) and at high ionic strength than in the presence of Mg(2+) and at low ionic strength. Preliminary experiments show that prostatic nucleolar RNA polymerase transcribes prostatic chromatin poorly as compared with the nucleoplasmic enzyme. The observations reported indicate an involvement of non-histone proteins associated with DNA in the process by which stimulation of enzyme activity by the 5alpha-dihydrotestosterone-receptor complexes is achieved. The implications of these findings in the mechanism of steroid hormone action is considered.


Synthesis and degradation of mitochondrial components in hypertrophied rat heart. The accumulation of inner mitochondrial components of rat heart was studied 1 and 3 days after constriction of the ascending aorta of rats. By 1 day after aortic constriction, the activities of three mitochondrial respiratory enzymes/mg of cardiac homogenate protein were increased; after 3 days, specific activities had levelled off or decreased. Selective accumulation of inner mitochondrial membrane components 24h after aortic constriction was further indicated by increased left ventricular cytochrome c concentration (nmol/mg of protein). By 3 days after surgery, cytochrome c concentration was significantly diminished. Low-temperature spectroscopy of isolated mitochondria showed that the ratios of cytochromes c, b and a+a(3) remained unchanged after aortic constriction, suggesting that cytochrome c was a good indicator of the response of the other mitochondrial inner-membrane cytochromes as well. The effect of cardiac hypertrophy on the turnover of cytochrome c was also examined. Cytochrome c was labelled in its haem group with delta-amino[2,3-(3)H(2)]laevulinate 3 days before aortic constriction. By 1 day after surgery the total ventricular radioactivity in cytochrome c of aortic banded animals was significantly higher than in sham-operated controls, indicating a decreased degradation rate in the former during the first postoperative day. delta-Aminolaevulinate was shown to be a particularly suitable precursor for such turnover studies, since it results in rapid pulse-labelling of cytochrome c (peak activity in 90min), is rapidly removed from the precursor pool (t((1/2))=30min) and is not reutilized.