The effect of Ro 20-1724 upon induced beta-galactosidase synthesis in Escherichia coli.


The effect of 1-sarcosine, 8-leucyl angiotensin II on the pressor effect of infused angiotensin II.


The effects of diet cholesterol on the synthesis of rat serum apolipoproteins.


The effects of nicotinamide on mouse sleep.


The effects of some anti-inflammatory drugs on the acute-phase proteins in rheumatoid arthritis.


The effects of Lioresal on synaptic activity in the isolated spinal cord.


The effects of PGE1 on the spontaneous motility of the guinea pig and rat female reproductive tracts, in vitro.


The establishment of a line of visna virus-producing human astrocytes (V-1181N1).


The evolution of ovarian steroid hormones during luteolysis and folliculogenesis in the cow.


The face in sacred art of the Upper Sepik River of New Guinea: analogies to neuro-developmental aspects and to prosopagnosia. 3. Neuropathologic aspects: prosopagnosia.


The experimental pathology of cycad toxicity with special reference to oncogenic effects.


The formation of arabino nucleosides from 3-acetamido-1,2-di-O-acetyl-3,5-dideoxy-d-ribofuranose during the fusion synthesis.


The fructose 1,6-diphosphatase-phosphofructokinase substrate cycle. A site of regulation of hepatic gluconeogenesis by glucagon.


The glomerular basement membrane in long-term allografts with heavy proteinuria.


The hemicellulose of pollen grains of Phoenix dactylifera.


The immune response to virally determined tumor associated antigens.


The importance of DNA size for successful transfection of chicken embryo fibroblasts.


The influence of a conscious wish on dreams: a methodological study of dream meaning and function.


The influence of cholesterol on molecular motion in egg lecithin bilayers--a variable-frequency electron spin resonance study of a cholestane spin probe.


The in vitro synthesis of avian myeloblastosis viral RNA sequences. Isolated nuclei, prepared from myeloblasts of chicks infected with avian myeloblastosis virus, synthesize RNA sequences present in avian myeloblastosis viral RNA. These sequences are also formed during transcription of chromatin, isolated from myeloblasts, by DNA-dependent RNA polymerases purified from Escherichia coli or calfthymus. In the latter case, transcription is alpha-amanitin sensitive. Formation of hybrids between RNA and avian myeloblastosis virus DNA probes has been monitored by the combined use of ribonucleases A, T(1), and H, and ribonucleases specific for single strands.


The influence of supraoptimal temperature on poliovirus type 1 Mahoney strain reproduction.


The influence of the size of wuartz dust particles on the reaction of lung tissue.


The furocoumarin photosensitizing effect on the virus-producing Graffi leukaemia cells.


The interaction of cyclic 3',5'-adenosine monophosphate with yeast glyceraldehyde-3-phosphate dehydrogenase. I. Equilibrium dialysis studies.


The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins.


The involvement of human placental microsomal cytochrome P-450 in aromatization.


The isolation of chemically pure elastins in a form suitable for mechanical testing.


The isolation of soluble proteins, glycoproteins, and proteoglycans from bone.


The mechanism of water-proton relaxation in enzyme paramagnetic-ion complexes. 1. The Gd(3)-lysozyme complex.


The isolation of terminally cross-linked DNA and kinetics of venom phosphodiesterase.


The metabolism of 25-hydroxycholecalciferol by isolated renal tubules in vitro as studied by a new chromatographic technique.


The influence of the vegetative nerve system on the maximal conduction velocity in the atrioventricular bundle and in the cardiac ventricles measured by the differential quotient of QRS. Investigations in dogs.


The modulating effect of cholera enterotoxin on the immune response.


The phosphorylation and subsequent metabolism of 1-aminopropan-2-ol.


The polyoma pseudovirion--its properties, production, and use in transferring DNA to mouse and human cells.


The nucleases from Acrocylindrium sp. IV. Purification and properties of exonuclease.


The polypeptide compositions of bovine cytochrome oxidase and its "proteinase-treated" derivative.


The preparation of 1-deoxy-1-halogeno-D-fructoses.


The properties and origin of the proteins in the SV40 nucleoprotein complex.


The protective effects of nucleotides on the histamine hypersensitivity of Bordetella pertussis-treated mice.


The purification of glycerate kinase from Hyphomicrobium sp. and Pseudomonas AM1: product identification.


The redox properties of the cytochromes of purified complex 3.


The regulation of polyamine synthesis during the stringent control in Escherichia coli.


The renin-angiotensin system and aldosterone secretion during sodium depletion in the rat.


The role of cyclic 3'5'-AMP and its derivatives in lipid and carbohydrate metabolism.


The role of calcium in insulin action. I. Purification and properties of enzymes regulating lipolysis in human adipose tissue: effects of cyclic-AMP and calcium ions.


The role of the amino group in subunit association and receptor site interaction for ovine luteinizing hormone as studied by acylation.


The role of the secretory immune system in protection against agents which infect the respiratory tract.


The susceptibility of the fetus and child to chemical pollutants. Viral etiology of cancer: two hypotheses with relevance to chemical exposures.


The state of water on hydrated collagen as studied by pulsed NMR.


The synthesis and biological activity of peptides corresponding to the first nineteen amino acids of adrenocorticotropin containing delta-aminovaleric and omega-aminocaprylic acids.


The synthesis of virus-specific polypeptides on polyribosomes isolated from the cells infected with encephalomyocarditis virus.


The toxic optic neuropathies.

The toxic optic neuropathies.


The ultrastructure of erythropoiesis in two haemoglobinopathies.


The use of lymphangiography in melanoma.


The use of conjugated oestrogen in oral contraception. To find a suitable contraceptive combination 11 dosage combinations of estrogen and megestrol acetate were studied clinically.  An additional combination for treatment of menopausal symptoms was used in 603 treatments, each with 21 pills.  A dose of 3.75 mg and even 4.0 mg of conjugated estrogen was not sufficient to inhibit ovulation.  Pregnancies occurred also when 21 daily doses of 3 mg conjugated estrogen were combined with 2 or 3 mg megestrol acetate daily.  Consequently, not even 3 mg megestrol acetate can be considered a sufficient dose to prevent the pentration of spermatozoa through the cervical mucus when used in combination with estrogens.  Clinically best results were obtained with following 2 combinations (2 and 7): conjugated estrogen 3.75 mg/day for 21 days + 5 mg megestrol acetate for the last 5 days and conjugated estrogen 4 mg/day for 21 days + 5 mg megestrol acetate for  the last 7 days.  The tolerance to conjugated estrogen was good in the whole series.  No embolism was observed, nor were any changes in the ASAT; GOT or ALAT; GPT values recorded.


The value of resection in tumors involving the chest wall.


Theoretical analysis of the oxidation of amylose by periodate ion as an example of cooperative effects in polymer kinetics.


Threshold of the early components of the averaged electroencephalic response determined with tone pips and clicks during drug-induced sleep.


Thyrotropin-induced aggregation-promoting factors of adult cultured thyroid cells.


Tissue cytochrome oxidase activity and copper, iron, and pantothenic acid nutrition in the growing rat.


Traffic of metabolites between blood and tissue.


Transformation of hamster embryo and human embryo cells by temperature sensitive mutants of herpes simplex virus type 2.


Transformation-defective virus mutants in a class of morphologic revertants of sarcoma virus transformed nonproducer cells.


Transfusion-acquired cytomegalovirus infection in newborn infants.


Transplantation of nervous system tumors in diffusion chambers.


Translation in vitro of total nuclear RNA from HeLa cell nuclei infected with adenovirus 2. Total heterogeneous nuclear RNA from HeLa cells infected with adenovirus for 18-20 hr stimulates amino acid incorporation into protein in a cell-free system from Ehrlich ascites tumors. This stimulation of protein synthesis by the nuclear RNA requires intact nuclei. The role of nuclei in this system is unknown, but evidence is presented that the nuclei are involved in the conversion of high-molecular-weight RNA to low-molecular-weight species. Some of the newly synthesized polypeptides appear to resemble the virion polypeptides.


Transport of ACTH across human placenta.


Traumatic induction of an intraoral reinfection with herpes simplex virus. Report of a case.


Transport of lipids in insects.

Transport of lipids in insects. Many insect species are almost completely dependent on lipids for their metabolic needs, although this is usually a function of developmental stage. The primary storage organ is the fat body, which can constitute 50% of the fresh weight of the insect and also acts as the major metabolic center (analogous to the vertebrate adipose tissue and liver). Bathing the fat body (and all other tissues and organs) is the hemolymph, the main functions of which are to transport nutrient substrates to utilization sites and to deliver metabolic wastes to the excretory system. Although neutral lipids are stored as triglycerides, in times of need they appear to be endergonically released into the hemolymph as diglycerides in the majority of insects thus far studied (particularly silkmoths and locusts). Indeed, diglycerides constitute the largest neutral lipid fraction in the hemolymph of silkmoths, locusts, cockroaches, bugs, etc. In the hemolymph the diglyceride is found as a constituent of specific lipoproteins, and one specific lipoprotein class (lipoprotein I; high density lipoprotein) appears to be necessary for the transport of diglyceride from the fat body cell into the hemolymph. This particular lipoprotein is also involved in the transport of cholesterol from the gut into the hemolymph. Thus, lipoprotein I appears to be the major neutral lipid and sterol transport agent in the insects studied and, in addition, plays a regulatory role in the release of both diglycerides and sterols. Hemolymph lipoprotein II (very high density lipoprotein) may be important in providing protein and lipid to the insect ovary during oogenesis. Ecdysone, the polyhydroxy steroidal insect molting hormone, is probably carried "free" in the hemolymph, although reports exist of specific hemolymph-binding proteins in some species. The other major insect growth hormone, juvenile hormone, is transported by hemolymph lipoproteins in silkmoths and locusts and by a lower molecular weight hemolymph protein in the tobacco hornworm.


Trophoblastic disease diagnosis and management.


Treatment of acute cholecystitis by routine urgent operation.


Tumor viruses at Cold Spring Harbor.

Tumor viruses at Cold Spring Harbor.


Turnover of phosphatidylglycerol in Streptococcus sanguis.


Two cases of pseudo-gout.

Two cases of pseudo-gout.


Two forms of mouse interferon messenger RNA.


Two routes for synthesis of phosphoenolpyruvate from C4-dicarboxylic acids in Escherichia coli.


Ultrastructural changes in the smooth musculature of antrum and pylorus in presence of ulcus ventriculi.


Transient effect of angiotensin II infusion into the lateral ventricle of the brain on the blood ADH level in dogs.


Ultrastructural evidence for leakage of lysosomal contents after phagocytosis of monosodium urate crystals. A mechanism of gouty inflammation. To obtain direct evidence for the mechanism involved in gouty inflammation, human leukocytes were incubated with synthetic monosodium urate microcrystals. To trace the phago(lyso)somal contents, colloidal carbon, ferritin, Thorotrast(R) or horseradish peroxidase was added to the incubation medium, or acid phosphatase activity was localized cytochemically. The interaction was analyzed in time sequence by electron microscopy. By 5 minutes' incubation, urate crystals and tracers had appeared in the single membrane-bounded vacuoles of leukocytes. After 30 minutes' incubation, the vacuoles containing the urate crystals and the tracers were found in more than 50% of the leukocyte population. The phago(lyso)somal membrane was occasionally discontinuous, and the tracers were found in the free cytoplasm near the membrane opening as well. After 60 minutes' or more incubation, the phago(lyso)somal changes and the cytoplasmic localization of the tracers were common, and many cells showed signs of degeneration. Urate crystals mixed in cell debris were often found to be ingested by other leukocytes. These results have been interpreted as follows. Monosodium urate crystals are avidly phagocytized. The urate crystal-containing phagosomes eventually become phagolysosomes. The phagolysosomal membrane is damaged and the contents leak out into the cytoplasm. The leakage of the hydrolases initiates the host cell injury. The urate crystals released by cell disruption are again phagocytized by other cells and the series of events are repeated.


Ultrastructural studies of early nidation in pregnancy and pseudopregnancy.


Ultrastructure of the inclusion bodies and nuclear abnormalities in beta-thalassemic erythroblasts.


Ultrastructure of tumors induced in mice by Moloney and Harvey strains of murine sarcoma virus.


Unit responses in the hypothalamus.

Unit responses in the hypothalamus.


Unstable hemoglobins and thalassemia: some properties of Hb Köln.


Ureteral and vesical metastases in nephroblastoma.


Uptake of 2-deoxyglucose by BALB-3T3 cells: changes after polyoma infection.


Use of stochastic signals in evaluation of the dynamic properties of a neuronal system.


Use of unithiol for reducing radiation hazard of renal scintigraphy with chlormerodrin 203Hg. I. Estimation of the urinary excretion of 203Hg.


Use of unithiol for reducing radiation hazard of renal scintigraphy with chlormerodrin 203Hg. II. Scintigraphic estimation.


Value structure as a predictor of type of discharge among residential addicts.


Variable sensitivity of different hypertensives to constant infusion of angiotensin.


Variables related to success in a medical rehabilitation setting.


Various aspects of alpha-thalassemia.

Various aspects of alpha-thalassemia.


Varicella dendritic keratitis.

Varicella dendritic keratitis.


Vastus lateralis cytochrome oxidase activity and its relationship to maximal oxygen consumption in man.


Very early effects of aminonucleoside on some rat liver enzymes.


Vesicular stomatitis virus envelope glycoprotein alterations induced by host cell transformation.


Virilization in pregnancy associated with polycystic ovary disease.


Virogenic nontransformed cells isolated following infection of normal rat kidney cells with B77 strain Rous sarcoma virus.


Virus antibody in synovial fluid of rheumatoid and control patients.


Vitamin D physiology and its importance in dairy cattle: a review.


Visual loss: a rare complication following oil myelography. Case report and review of the literature.


Waxes of the uropygial gland secretion of birds of the genus Parus.


Vitamin D: mode of action and biomedical applications.


Workshop on possible pharmacologic mechanisms for enhancement of lung maturation.


14CO2 fixation and glycolate metabolism in the dark in isolated maize (Zea mays L.) bundle sheath strands.


1Alpha-hydroxyvitamin D3. An analog of vitamin D which apparently acts by metabolism to 1alpha, 25-dihydroxyvitamin D3.


3-Epi-20-hydroxyecdysone from meconium of the tobacco hornworm.


What do women want?

What do women want?


8-(6-Aminohexyl)-amino-adenine nucleotide derivatives for affinity chromatography.


Some applications of calorimetry in biochemistry and biology.


U.S. childhood cancer deaths by cell type, 1960-68.


What cyclic AMP is all about.

What cyclic AMP is all about.


High density lipoproteins in cholestasis.


Some diseases of zoo animals.

Some diseases of zoo animals.


Some hematologic and biochemical observations in the mongoose (Herpestes auropunctatus).


On a family with coexistence of phenotypes II and III hyperlipoproteinemia.


Cell inoculum size and rate of subsequent exponential multiplication in human cell lines.


Role of cyclic nucleotides in the nervous system.


Role of cytochrome b5 in hydroxylation by a reconstituted cytochrome P-450-containing system.


Role of lipid in the formation and function of the respiratory system of Staphylococcus aureus.


Loss of mitotic response of the thyroid gland to TSH in hypophysectomized rats and its partial restoration by anterior and posterior pituitary hormones.


Role of splenic arteriography in evaluation of malignant tumors in the body of the pancreas.


Role of the amygdaloid complexes in the stress-induced release of ACTH in the rat.


Role of phospholipids in transport and enzymic reactions.


Role of 3-mercaptopyruvate sulfurtransferase in the formation of the iron-sulfur chromophore of adrenal ferredoxin.


Some properties of Ascaris suum allergen A.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 48-1974.


Host responses to infectious bovine rhinotracheitis virus. III. Isolation and immunologic activities of bovine T lymphocytes.


Fine structure of the adrenal gland in the duck (Anas platyrhynchos).


Poly(riboadenylic acid) and adjacent nucleotides in Rous sarcoma virus RNA.


Host-cell reactivation in mammalian cells. IV. Cell culture conditions affecting virus survival.


Cell-mediated immunity to varicella-zoster virus: in vitro lymphocyte responses.


Drug-induced phospholipidosis.

Drug-induced phospholipidosis.


Drug-receptor-related stereoselectivities in fat cells.


Post-transfusion hepatitis in a London hospital: results of a two-year prospective study. A report to the M.R.C. Blood Transfusion Research Committee by the Medical Research Council Working Party on Post-Transfusion Hepatitis. Seven hundred and sixty-eight patients were seen and tested at frequent intervals after transfusion of whole blood. Eight patients were judged to have developed icteric or anicteric post-transfusion viral hepatitis, an incidence of 1%. Five were icteric and four of these were hepatitis B antigen (HB Ag) positive; two of these four died. One of the fatal cases and one non-fatal HB Ag positive case had received HB Ag positive blood. Two other antigen-positive patients had received blood or plasma or both which had not been tested for antigen.Thirty-five patients showed conspicuous or sustained elevations of alanine transaminase without clinical features of hepatitis.Four were positive for HB Ag but had not received antigen positive blood.Two who had received antigen positive blood remained antigen negative, but one developed hepatitis B antibody (HB Ab).Two other patients were also transfused with plasma.Five had serological evidence of cytomegalovirus (CMV) infection accompanying the enzyme changes.One patient who had received HB Ag positive blood remained antigen-negative and showed no abnormalities.Five patients who became HB Ag positive, although they had been given antigen-negative blood, remained clinically and biochemically well.Cytomegalovirus primary infection or reactivation occurred in another 32 patients; five had isolated, transient enzyme rises, one other was associated with a drug-induced focal liver necrosis and 26 showed no enzyme changes. Epstein-Barr virus infections, one of which was associated with a transient upset of enzyme activity, were detected in five patients. There were no cases of post-perfusion syndrome.


Visna-maedi in sheep.

Visna-maedi in sheep.


Destabilization of membranes with chaotropic ions.


Surface proteins of the erythrocyte membrane. Effect of aging.


Intracellular activity of medullary respiratory neurons.


Extracellular toxins of Pseudomonas aeruginosa.


Avenaciolide inhibition of anion transport in mitochondria.


Intracisternal inclusions in nerve cells of cat ventromedial hypothalamic nucleus.


Characteristic ultrastructural changes in organs of newborn mice infected with EMC virus.


Dose-response analysis of angiotensin- and renin-induced drinking in the cat.


Characterization and comparison of rabbit blastokinin produced under various physiological and experimental conditions.


Characterization of the fraction obtained from the CNS of Jimpy mice by a procedure for myelin isolation.


Characterization of the insulin and somatomedin-C receptors in human placental cell membranes.


Characterization of Bacillus polymyxa amylase as an exo-acting(1 leads to 4)-alpha-D-glucan maltohydrolase.


Degradation of oil shale by sulfur-oxidizing bacteria. Approximately 40% of oil shale can be solubilized by the action of sulfur-oxidizing bacteria. Thiobacillus thiooxidans and Thiobacillus concretivorous are equally effective in solubilization. Continuous leaching experiments show that this process can be completed within 14 days. The growth of Thiobacillus and the production of acid were measured under several conditions. Almost all of the CaMg(CO(3))(2) was removed by this process, leaving a complex of silica and kerogen that could be burned as low-energy fuel. The silica-kerogen complex had not yet been biologically degraded.


Circadian variation in human urinary cyclic AMP and the effect of different diets on this rhythm.


Intradiaphragmatic cyst: a diagnostic challenge.


Estradiol receptor in the human fallopian tube.


Estradiol receptors in the oviduct and uterus of the rabbit.


Leukaemogenic effect of repeated inoculations with small doses of Tennant virus in BALB-c mice.


The aggregation of rabbit polymorphonuclear leucocytes: effects of antimitotic agents, cyclic nucleotides and methyl xanthines.


Spinal pathways for cortical influences on lumbar motoneurons in rats.


Spinal transmission of autonomic processes.


Isovaleric acid as a precursor of odd numbered iso fatty acids in Tetrahymena.


Mammalian acyl carrier protein. Dissociation of the acyl carrier protein subunit from dog liver fatty acid synthetase complex.


A team approach to a disease survey on an aboriginal island (Orchid Island, Taiwan). V. Arboviral, rickettsial and brucella antibodies on Orchid Island.


Glutamate dehydrogenase activity related to histopathological grade of hepatocellular carcinoma in man.


Monoaminergic enzymes other than MAO.

Monoaminergic enzymes other than MAO.


Neuraminidase treatment of adrenal cells increases their response to cholera enterotoxin.


Monoamines and the regulation of vigilance and sleep.


Neuraminidase: its effect on synaptic transmission.


Manganese in photosynthetic oxygen evolution. I. Electron paramagnetic resonance study of the environment of manganese in Tris-washed chloroplasts.


The anatomy and physiology of the oviduct.


Methanesulfonic acid derivative of p,p'-diaminodiphenylsulfone. II. Pharmacokinetics and availability of parental drug in rabbit.


Cholangiocarcinoma: a clinicopathologic study of five cases with ultrastructural observations.


Mechanically self-induced muzzle alopecia in mice.


Intramitochondrial localization of arginase appearing in chicken liver upon starvation.


Mechanism of stimulation of murine type-C RNA tumor virus production by glucocorticoids: post-transcriptional effects. We have previously shown that dexamethasone stimulates production of type-C virus from seemingly normal murine fibroblasts (BALB/3T3) and from transformed (Kirsten sarcoma-leukemia virus) nonproducing cells (BALB/K3T3) induced by 5-iododeoxyuridine. In this report, we further examine the mechanism of this effect by using BALB/K3T3 cells. Several observations suggest that this effect is post-transcriptional. The optimal stimulation by dexamethasone is obtained when dexamethasone is given 24 to 48 h after 5-iododeoxyuridine induction. Although this effect is late, time course experiments suggest that dexamethasone does not act to promote release of preformed virions. The stimulation by dexamethasone is blocked when cells are treated with cordycepin (3'-deoxyadenosine) during the first 24 h of induction, but not when cordycepin is added later. Conversely, interferon, which inhibits virus production, interferes with dexamethasone when it is added late or after removal of the steroid. The results of molecular hybridization experiments show that there is no detectable increase in Kirsten sarcoma-leukemia virus-specific RNA in dexamethasone-treated cells (with or without 5-iododeoxyuridine). The results of the time course studies, and the cordycepin, interferon, and hybridization experiments, suggest that the effect of dexamethasone on type-C virus production in this system is post-transcriptional.


Mechanisms by which activated normal macrophages destroy syngeneic rat tumour cells in vitro. Cytokinetics, non-involvement of T lymphocytes, and effect of metabolic inhibitors.


Mechanisms of blood-vascular reactions of the primate lung to acute endotoxemia.


Mechanisms of inflammation.

Mechanisms of inflammation.


Mechanisms for development of diabetic hypertriglyceridemia in streptozotocin-treated rats. Effect of diet and duration of insulin deficiency. A combined ultrastructural and functional approach was employed to define the effects of duration of diabetes and of diet on various aspects of lipid metabolism in rats with severe streptozotocin (SZ)-induced insulin deficiency. Plasma triglyceride (TG) levels rose to a mean of 479 mg/100 ml 24 h after SZ administration in rats eating a fat-free, high carbohydrate diet as compared to a mean of 324 mg/100 ml in rats eating a high fat diet. These changes were associated with a commensurate increase in hepatocyte Golgi very low density lipoprotein (VLDL) content, but only a small increase in estimates of VLDL-TG secretion rate (post-Triton WR 1339 increment in plasma TG level). Although these findings are consistent with the thesis that VLDL-TG synthesis and secretion are increased 24 h after administration of SZ, it seemed unlikely that the observed increase in VLDL-TG secretion could entirely account for the severity of the hypertriglyceridemia. Thus, although lipoprotein removal rate was not measured directly, it was necessary to postulate that a defect in VLDL-TG removal was also present at this stage. Hypertriglyceridemia was still present 7 days later, only in this instance plasma TG levels were higher in rats eating the high fat diet (a mean of 589 mg/100 ml, as compared to 263 mg/100 ml). Rats with diabetes of 7-day duration had a 50% decrease in both TG entry rate and hepatocyte Golgi complex VLDL content, irrespective of diet. Thus, there was no evidence of increased VLDL-TG secretion in chronic insulin deficiency. In this instance, although not assessed directly, it was necessary to postulate that the hypertriglyceridemia in chronically insulin-deficient rats is due entirely to a defect in lipoprotein removal, involving both dietary and endogenous fat.


The antiviral activity of some related benzo(b)thiophene derivatives. I. Initial screening tests.


Ocular malformations in chick embryo resulting from diminished intraneural pressure.


Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes.


Comparative immunogenicity of irradiated, neuraminidase treated, and fused cells of a strain-restricted sarcoma.


Preparation of isolated fat cells and fat cell "ghosts"; methods for assaying adenylate cyclase activity and levels of cyclic AMP.


Comparative studies of viral infections of the developing forebrain. II. Electron microscopic studies of rat virus and bluetongue vaccine virus infections.


Comparative studies of viral infections of the developing forebrain. I. Pathogenesis of rat virus and bluetongue vaccine virus infections in neonatal hamsters.


Univariate and multivariate models for evaluating long-term changes in neurobiological development, maturity and aging.


Suprarenal cortical reserve capacity in Kwashiorkor.


Comparison between effects of cyclic AMP, dibutyryl cyclic AMP and luteinizing hormone on ovarian glycolysis.


Comparison of adenylate kinase from liver and muscle of normal mice and those with an hereditary muscular dystrophy.


Comparison of antigenicity of hepatoma cells, normal liver cells, foetal liver cells and chemically damaged liver cells in guinea-pigs immunized with hepatomata using the macrophage migration inhibition test. The macrophage migration inhibition test has been used to study the immune responses of guinea-pigs immunized with injections of whole cells of both an allogeneic and a syngeneic hepatoma grown as established cell lines in tissue culture.A clear dose-response relationship between tumour cell concentration and migration inhibition was seen in immunized animals and no significant migration inhibition was seen in control animals. There was no cross reaction between the two tumours used. There was no cross reaction between whole isolated normal liver cells and tumour cells, or between foetal liver cells and tumour cells. Whole isolated liver cells from carbon tetrachloride damaged livers caused some degree of migration inhibition in both normal and immunized guinea-pigs but, taking this into account, they did not appear to cross react with hepatoma cells.


Urinary cyclic adenosine monophosphate excretion in diabetes insipidus of childhood. Effect of vasopressin and chlorpropamide.


Comparison of the fractional post-coital test with the Sims-Huhner post-coital test.


Urinary excretion of adenosine 3' :5'-cyclic monophosphate in depressive illness.


Primary hyperparathyroidism. Preoperative evaluation and correlation with surgical findings.


Primary linitis plastica carcinoma of the colon.


Primary malignant hemangioendothelioma of the liver. Report of a successful case of resection and review of the Japanese literature.


Primary reninism, a surgically curable form of hypertension.


Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.


Urinary steroid dynamics after a single dose of metyrapone.


Ultrastructural effects of dietary galactoflavin on mouse hepatocytes.


Ultrastructural and biochemical studies of the isolated fibrillar component of nucleoli from Novikoff hepatoma ascites cells.


Ultrastructural changes in the nervous system after chronic exposure to halothane.


Ultrastructural features of papillomatosis and carcinoma of nipple ducts. The significance of myoepithelial cells and basal lamina in benign, "questionable," and malignant lesions.


Ultrastructural observation of possible nerve endings in rat sebaceous gland.


Ultrastructural observations on the spinal cords of piglets affected with congenital tremor type AIV.


Ultrastructure and three-dimensional organization of synaptic glomeruli in rat somatosensory thalamus.


Ultrastructure of Michaelis-Gutmann body. A study of a case of testicular malacoplakia.


An easy radioimmunological microassay of renin activity, concentration and substrate in human and animal plasma and tissues based on angiotensin I trapping by antibody.


Ultrastructural evolution of experimentally induced lipid inclusions in rat and mouse hepatocytes indicative for the recovery of livers from effects of benzidamine and triacetyloleandomycin.


Ultrastructure of the lamina propria in the ovine seminiferous tubule. Development and some endocrine considerations.


Neonatal changes in the concentration of rat liver cyclic AMP and of serum glucose, free fatty acids, insulin, pancreatic, and total glucagon in man and in the rat.


Neonatal keratitis due to herpes virus hominis type i infection.


Neonatal plasma lipids as measured in cord blood. The status of cord blood screening of plasma lipids is reviewed, emphasizing the problems associated with the diagnosis of familial hyperlipoproteinemia (HLP), particularly type II (hyperbetalipoproteinemia), in the neonate. For 2937 neonates in this study the mean +/- standard deviation (SD) of plasma cholesterol was 70.3 +/- 16.9 mg/dl. The mean triglyceride level of 1805 neonates was 39.6 +/- 19.3 mg/dl. The mean level of beta-cholesterol in 240 neonates was 34.3 +/- 10.9 mg/dl and the correlation between total and beta-cholesterol was high (r=0.89). By convention, the upper limit of normal was defined as the mean + 2 SD, and these levels for total cholesterol, triglyceride and beta-cholesterol were 100, 80 and 55 mg/dl respectively. Only by careful follow-up of hyperlipidemic neonates can the incidence of HLP and the worth of early diagnosis be assessed.


Repeated examinations, using the laparotomy sampling technique, of the gastro-intestinal microflora of baboons fed a natural or a synthetic diet.


Isolation and characterization of two distinct types of HVJ (Sendai virus) spikes.


Isolation and characterization of rifampin-resistant and streptolydigin-resistant mutants of Bacillus subtilis with altered sporulation properties. Mutants of Bacillus subtilis with altered deoxyribonucleic-dependent ribonucleic acid polymerase activity have been isolated and characterized. These mutants, selected as strains resistant to rifampin or streptolydigin, demonstrate drug-resistant in vitro ribonucleic acid synthesis. Sporeforming ability and support of phage infection are altered in many of the mutants. Mutations to rifampin and streptolydigin resistance have been located on the B. subtilis chromosome and ordered relative to the markers cysA14 and str.


Radiation effects on NAD-and DNA-metabolism in mouse spleen.


Isolation of hen's egg yolk very low density lipoproteins by DEAE-cellulose chromatography.


Isolation of kidney lysosomes.

Isolation of kidney lysosomes.


Isolation of modified liver lysosomes.

Isolation of modified liver lysosomes.


Isolation of myelin from nerve tissue.

Isolation of myelin from nerve tissue.


Isolation of nuclei from liver and other tissues.


Isolation of plasma membranes from rat and mouse livers and hepatomas.


Isolation of rat liver plasma membrane fragments in isotonic sucrose.


Variation of serotype in strains of Bordetella pertussis. The four main serotypes of Bordetella pertussis (1, 2, 3; 1, 2; 1, 3; 1) undergo spontaneous variation involving loss or gain of antigen 2 or antigen 3. By serial subculture from single colonies on charcoal-blood-agar medium, we have detected loss-mutations from type 1, 2, 3 to 1, 2 or 1, 3, and from type 1, 2 to type 1. Likewise we have found gain-mutations from type 1 to 1, 2 or 1, 3, and from 1, 2 to 1, 2, 3.These mutations apparently occur with a high frequency in some strains. Other strains have a lower mutation-rate and are more stable antigenically. We have not detected, by this method, either gain- or loss-mutations from the type 1, 3 strains that we have tested.These findings offer an explanation for the changes in serotype that occur during the course of a pertussis infection in the child and in the marmoset. They also constitute a warning on the possible antigenic instability of laboratory strains, especially relevant in the production, absorption and testing of diagnostic antisera and in the preparation of pertussis vaccine.


Isolation of ACTH-induced protein from adrenal perfusate.


Radiation therapy in bronchiolar-alveolar cell carcinoma: a case report.


Formation of 5'-nucleotides of 6-methylmercaptopurine ribonucleoside in human tissues in vitro.


Isolation, identification, and characterization of six bovine picornavirus isolates.


Variations of cytotoxic antibodies to cells with herpes simplex virus antigens in women with progressing or regressing cancerous lesions of the cervix.


Chelator-sensitive in chloroplast electron transport.


The background to Arthur Harden's discovery of cozymase.


Solubilization of membrane-associated tumour-specific antigens by beta-glucosidase.


Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media.


Inhibition of cytochrome c oxidase by hydrophobic metal chelators.


Inhibition of growth of gonadal dependent structures by arginine vasotocin and purified bovine pineal fractions in immature mice and hamsters.


Inhibition of Flac transfer by the fin+ I-like plasmid R62. Flac mutants have been isolated in Escherichia coli K-12 which carry dominant mutations resulting in insensitivity to transfer inhibition by the Fin(+) I-like plasmid R62. These mutants were still sensitive to transfer inhibition by the fin(+) F-like plasmid R100 and, conversely, FlactraO(-) and traP(-) mutants, which are insensitive to R100 inhibition, were still sensitive to R62. The sites of action of the two inhibition systems are therefore different. Furthermore, inhibition by R62, unlike R100, did not require an F-specified product. Like R100, R62 prevented transfer, pilus formation, and surface exclusion and, therefore, probably inhibits expression of the transfer operon traA through traI. However, R62 was different from R100 in inhibiting transfer of J-independent mutants, indicating that its effect on the transfer operon is probably direct rather than via traJ. This is consistent with the different sites of action of the two inhibition systems. None of the Flac mutants overproduced pili in the absence of R62, although one mutant differing from those described above showed increased levels of transfer and surface exclusion.


Amoebic liver abscess--a clinical study.


Antibodies to herpes-simplex virus in the cerebrospinal fluid of patients with herpetic encephalitis.


Antibodies to Epstein-Barr virus and some other herpesviruses in patients with sarcoidosis, pulmonary tuberculosis and erythema nodosum.


Metabolic effects of cortisol and corticotrophins in the Australian brush-tailed opossum, Trichosurus vulpecula (Kerr).


Metabolic regulation by multifunctional glucose-6-phosphatase.


Metabolic adaptation of spiny mice (Acomys cahirinus) transferred from the desert to laboratory diets.


Metabolic studies on 125I-labeled baby hamster kidney cell plasma membranes.


Metabolism of iodinated very low density lipoprotein in the rat. Autoradiographic localization in the liver.


Metabolism of plasma triglycerides in endogenous hypertriglyceridemia.


Foodborne disease surveillance: Washington State.


The bovine respiratory disease complex.

The bovine respiratory disease complex.


A rubredoxin-like mononuclear FeS4 derivative of adrenal iron-sulfur protein (adrenodoxin).


Cerebrocerebellar communication systems.


L-Ribulokinase from an initiator constitutive mutant. The N-terminal sequence of l-ribulokinase (EC 2.7.1.16) isolated from an initiator constitutive (araI(-)) mutant is identical to wild-type kinase, indicating that the I(c) mutation probably lies outside of kinase structural gene araB.


99mTc pyrophosphate for diagnostic bone imaging.


Radical treatment of recurrent cytosarcoma phylloides. Malignant cystosarcoma phylloides is a rare breast cancer which frequently recurs locally before metastasizing. Local recurrence of cystosarcoma phylloides is unlike adenocarcinoma of the breast since it does not necessarily indicate systemic metastases; and, therefore, aggressive surgery can cure a number of patients with locally recurrent tumor. The present report is an extreme example of a patient with locally recurrent malignant cystosarcoma phylloides, and it illustrates the value of radical surgery in controlling this disease which had previously recurred 21 times. Fifteen months following sternal resection, the patient enjoys her longest tumor-free interval in 16 years.


A receptor mediating sexual differentiation?


Bronchial carcinoma. A retrospective analysis of 2200 patients.


Capacitation in vitro of rabbit spermatozoa with cyclic adenosine monophosphate and human follicular fluid.


Principles of treatment of different types of parotid tumors.


The clinical effects of CI 744 in chinchillas, Chinchilla villidera (Laniger).


The coordination of sterically hindered bases to metalloporphyrins.


Two cases of chemodectoma of the carotid body.


Endocrine evaluation and indications for surgery of functional pituitary adenomas.


Cytochrome oxidase activity in isolated giant cell of the reticular formation from the rat brain stem following carbon monoxide intoxication (Cartesian micro-diver technique).


Direct binding of corticosterone and estradiol to rat liver nuclear non-histone proteins.


The control of aldosterone secretion in nephrectomized man.


Effect of aging, organ allografting, and surgical stress on rat thymus histidine decarboxylase activity.


Effect of beta-propiolactone inactivation of polyoma virus on viral functions. Polyoma virus was inactivated by treatment with beta-propiolactone. T-antigen production, polyoma-RNA synthesis, induction of host DNA synthesis (measured by incorporation of labeled thymidine into the cell culture), and in vitro transforming ability were inactivated to a similar degree by various beta-propiolactone concentrations (0.25% beta-propiolactone reduced these functions approximately 96%), whereas plaque-forming ability and the ability of the virus to replicate its DNA and to synthesize capsid antigen were inactivated by a given concentration of beta-propiolactone to a much greater degree (0.25% beta-propiolactone led to a reduction of plaque-forming ability of over 8 logs). The significance of these data and their relationship to previously published experiments are discussed.


Direct determination of molar ratios of various chemical constituents in endotoxic glycolipids in silicic acid scrapings from thin-layer chromatographic plates.


Effect of different solid metals and metallic pairs on human sperm motility.


Effect of calcium ions on steroid-binding spectra and pregnenolone formation in rat-adrenal mitochondria.


Effect of cysteine on the survival of mice with transplanted malignant thymoma.


Effect of glucose on the fruiting body formation and adenosine 3',5'-cyclic monophosphate levels in Coprinus macrorhizus. The formation of fruiting bodies in the monokaryotic fis(c) strain and a dikaryon of Coprinus macrorhizus was inhibited by growth in high-glucose media. In high-glucose media the characteristic burst of adenosine 3',5'-cyclic monophosphate (cAMP) accumulation during fruiting-body formation was absent. Enzymatic activity assays revealed that mycelia grown in high-glucose media contained relatively lower amounts of adenylate cyclase and cAMP-phosphodiesterase than mycelia grown in low-glucose media. The synthesis of inducible d-serine deaminase and tryptophanase was repressed in high-glucose media. A mutant (gluR) in which the glucose repression of fruiting-body formation is affected was isolated by selection in high-glucose media. The mutation caused the cAMP levels to be no longer affected by glucose and affected ability to synthesize the inducible d-serine deaminase and tryptophanase. The gluR mutant was partially dominant in dikaryons. It is suggested that cAMP may play important roles in inducing fruiting bodies and in controlling inducible enzyme synthesis in C. macrorhizus.


Effect of inflammation upon human gingival oxidative metabolism.


Effect of intraventricular norepinephrine on neuron activity in the medial forebrain bundle during self-stimulation behavior.


Effect of inhibitors of proteolytic enzymes on the growth of normal and polyoma transformed BHK cells.


Effect of manganese extraction on oxygen generation and EPR signal II in spinach chloroplasts.


Effect of peritubular infusion of angiotensin II on rat proximal nephron function.


Effect of pentobarbital and sodium hydroxybutyrate on central mechanisms regulating vascular tone.


Effect of pharmacological agents on human keratinocyte mitosis in vitro. I. Inhibition by adenine nucleotides.


Effect of polyinosinic-polycytidylic acid in dogs experimentally infected with infectious canine hepatitis virus.


Effect of pregnancy on cytoplasmic and mitochondrial enzymes in human and animal myometrium.


Effect of secretagogues on membrane parameters of adenohypophysial cells.


Effect of sleep-wake cycle reversal on luteinizing hormone secretory pattern in puberty.


Effect of tumor-promoting phorbol esters on the normal and the isoproterenol-elevated level of adenosine 3',5'-cyclic monophosphate in mouse epidermis in vivo.


Effect of xanthothricin on the respiratory chain.


Effect of zinc deficiency from day 18 of gestation and-or during lactation on the development of some rat brain enzymes.


Effect of TSH on iodine metabolism and intermediary metabolism in tissue from patients with Graves' disease.


Effect of 1,3-butanediol on hepatic fatty acid synthesis and metabolite levels in the rat.


Effect of 2-nitrosonaphthalene on bacteria and bacteriophage.


Effect of various dietary fats on the size and distribution of lymph fat particles in rat.


Selected recent vistas in bone pathology.


Induction of aspermatogenesis in guinea pig through immunization with lactate dehydrogenase-x isozyme.


Detection of sequence heterology by use of the N. Crassa nucleases.


Detection of neoplastic lesions with radiogallium(67Ga citrate). Clinical-statistical report on 125 cases.


Induction of the postsynaptic membrane.

Induction of the postsynaptic membrane.


Detection of trophoblast specific antigen in normal and neoplastic trophoblast.


Infections of swine with pseudorabies virus and enteroviruses: laboratory confirmation, clinical and epizootiologic features.


Defective immune responses and malignancy.


Selective radioprotection in extracellular space.


Selective measurement of two lipase activities in postheparin plasma from normal subjects and patients with hyperlipoproteinemia. An assay has been developed for specific measurement of two different lipase activities in postheparin plasma. Lipoprotein lipase, derived from extrahepatic sources, is measured as protamine-inactivated lipase activity; hepatic lipase activity is protamine-resistant under the conditions of this assay. In 100 normal subjects, both enzyme activities were noted to be related to age and sex. Protamine-resistant lipase, which comprised 46-95% of the total activity, was highest in men over 18. Protamine-inactivated lipase activity was greatest in younger males and was age-correlated in women, doubling between the second and sixth decades. In 12 patients with hyperchylomicronemia, including five previously shown to have familial type I hyperlipoproteinemia, protamine-inactivated lipase activity was markedly reduced, whereas protamine-resistant lipase was below normal in only 1. The results were not due to lack of plasma activator, presence of plasma inhibitor, or diet, and the deficiency was not overcome by increasing the provoking dose of heparin from 10 U to 75 U/kg. Mean values for both lipase activities were not reduced in 32 other patients with hyperchylomicronemia, nine with "floating beta" lipoproteins (type III hyperlipoproteinemia), and 23 with hyperprebetalipoproteinemia (type IV). Mean protamine-resistant lipase activity was below normal in a group of four women with hypothyroidism, in whom protamine-inactivated lipase was not reduced. Both of the lipase activities were capable of hydrolyzing lipid in very low-density lipoproteins, but the relative rate of hydrolysis of chylomicrons by protamine-resistant lipase was markedly limited. These results indicate the importance of distinguishing between lipases of hepatic and extra-hepatic origin in the measurement of postheparin lipolytic activity.


Defective simian virus 40 genomes: isolation and growth of individual clones.


Effects of alpha- and beta-receptor blocking agents on catecholamine and 5-hydroxytryptamine induced peroxidase and amylase secretion from guinea pig submandibular gland.


Effects of buffers and pH on in vitro binding of 67Ga by L1210 leukemic cells.


Effects of clofibrate on mitochondrial function.


Effects of cyclic neucleotide monophosphates (cAMP, cGMP, cIMP, dibutyryl-cAMP) on transtubular net fluxes in rat kidneys.


Effects of dose on the induction of  dominant-lethal mutations and heritable translocations with ethyl methanesulfonate in male mice. Genetic damage by ethyl methanesulfonate (EMS) in male mice was measured at doses ranging from 50 to 300 mg/kg with dominant-lethal mutations and reciprocal translocations as endpoints. No appreciable increase in dominant-lethal mutations was detected following a dose of 100 mg/kg. Dominant lethals induced by EMS were convincingly detected only after a dose of 150 mg/kg, but in the translocation experiment an increase in the genetic effect was detectable at the 50 mg/kg dose. It is likely that dominant lethals had also been induced at the 50 and 100 mg/kg doses, but were not detected due to the relative insensitivity of the dominant..lethal procedure. Thus, for detection of low levels of EMS-induced chromosome breakage, translocations are a much more reliable endpoint than are dominant-lethal mutations. A procedure for large-scale screening of induced translocations is described.-The dominant-lethal dose-response curve, plotted on the basis of living embryos as a percentage of the control value, is clearly not linear as it is markedly concave downward. Similarly, the translocation dose-response curve showed a more rapid increase in the number of translocations with dose than would be expected on the basis of dose-square kinetics. It is clear for both of these endpoints that the effectiveness of EMS in inducing chromosome breakage is proportionately much lower at low doses.


Effects of dose on the induction of dominant-lethal mutations with triethylenemelamine in male mice. Dose effects of triethylenemelamine (TEM) in the induction of dominant-lethal mutations were studied at the early spermatozoon, midspermatid and spermatocyte stages. The pattern of effects on spermatocytes, unlike midspermatids and early spermatozoa, indicated possible cytotoxic damage, so for the determination of TEM dose-response curves in the induction of genetic damage only the data for midspermatids and early spermatozoa were used. The TEM dose-effect curves for those two stages differ markedly from ethyl methanesulfonate (EMS) dose-effect curves. Beginning with the lowest doses at which significant effects are observed, there is a considerably more rapid increase in dominant-lethal effects with dose of EMS than TEM. Another marked difference between the two compounds is in the ratio of the genetically effective dose (as measured by dominant-lethal mutations) to the lethal dose. The ratio is 1:100 for TEM and only 1:3.5 for EMS; thus, TEM is mutagenic far below its toxic level. Obviously, these results have important implications not only for our understanding of the nature of chemical induction and recovery of chromosomal aberrations but also for the practical problems of evaluating the mutagenic effects of chemicals.


Effects of gonadotrophins, dibutyryl cyclic AMP and prostaglandin E2 on amino acid transport and glycolysis in isolated rat corpora lutea.


Effects of intraventricular infusions of ACTH1-24 and ACTH4-10 on LH release, ovulation and behavior in the rabbit.


Effects of papaverine derivatives on cyclic 3',5'-AMP phosphodiesterase and relaxation of rabbit ileum.


Effects of pH and enzyme inhibitors on apparent generation of angiotensin I in human plasma.


Effects of progesterone and four synthetic progestagens on sodium balance and the renin-aldosterone system in man.


Effects of sympathetic stimulation on facial muscle contractions.


Effects of tiletamine on spinal cord synaptic transmission.


Effects on tumor growth in vivo by manipulation of the tumor immune response.


Defects of two temperature-sensitive lysyl-transfer ribonucleic acid synthetase mutants of Bacillus subtilis. Two temperature-sensitive mutants (lysS1 and lysS2) of the lysyl-transfer ribonucleic acid synthetase (l-lysine:tRNA ligase [adenosine 5'-monophosphate], EC 6.1.1.6) of Bacillus subtilis have been isolated. Although protein synthesis is inhibited in both mutants at the restrictive temperature (42 to 45 C), the mutants remain viable in a minimal medium. In comparison with the wild-type lysyl-tRNA synthetase, the l-lysine-dependent exchange of [(32)P]pyrophosphate with adenosine 5'-triphosphate (ATP) for both mutant enzymes is decreased. The lysS1 enzyme is completely defective in the ATP-dependent attachment of l-lysine to tRNA, whereas the lysS2 enzyme has 3- to 10-fold reduced levels of this activity. Temperature-resistant transformants have wild-type enzyme levels, whereas partial revertants to temperature resistance have varied levels of enzyme activity. The attachment and exchange activities of the lysS2 enzyme are more heat labile in vitro than the wild-type enzyme, as is the attachment activity of a partial revertant of the lysS1 mutant. The lysS1 and the lysS2 lysyl-tRNA synthetases have higher apparent K(m) values for lysine and ATP, in both the activation and the attachment reactions. The lysS2 enzyme has a V(max) for tRNA(lys) one-third that of the wild-type enzyme. Molecular weights of approximately 150,000 for the wild-type and lysS2 enzymes and approximately 76,000 for the lysS1 enzyme were estimated from sedimentation positions in sucrose density gradients assayed by the ATP-pyrophosphate exchange activity. We propose that the two mutations (lysS1 and lysS2) directly affect the sites for exchange activity, but indirectly alter attachment activity as a consequence of defective subunit association.


Effects of DMSO on vacuole formation, contractile vacuole function, and nuclear division in Tetrahymena pyriformis GL.


Structural studies of avian myeloblastosis virus: absence of leukemogenic activity of the core component.


Structural and functional heterogeneity of the murine cytomegalovirus genome.


Structure of polysaccharides from the Polyporus tumulosus cell wall.


Molecular and clinical pharmacology of psoriasis.


Molecular and crystal structure of deoxyguanosine 5'-phosphate.


Mixed mesodermal tumors of the corpus uteri.


Mixed substrate experiments with human brain monoamine oxidase.


Lipid transport and the availability of insulin.


Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method. The indirect immunoperoxidase technique with type-specific antisera was compared to kinetic neutralization for the typing of herpes simplex virus strains. There was complete agreement between the results obtained by kinetic neutralization and indirect immunoperoxidase. The indirect immunoperoxidase assay was far simpler to perform and interpret than the kinetic neutralization tests, and it offered a rapid means for the routine antigenic typing of herpes simplex isolates.


Tinidazole in symptomatic intestinal amoebiasis.


Accidental infection of laboratory-reared Macaca mulatta with Trypanosoma cruzi.


Ribodeoxyviruses and cancer.

Ribodeoxyviruses and cancer.


Mixed mesodermal tumour of the ovary.

Mixed mesodermal tumour of the ovary.


The dependence of evoked transmitter release on external calcium ions at very low mean quantal contents. 1. The mean quantal content of the frog end-plate potential was examined under conditions that reduced evoked transmitter release to very low values.2. When the calcium concentration of the Ringer is reduced below 10(-4)M a deviation occurs from the fourth power dependence of the mean quantal content (m) on the calcium concentration such that more quanta are released than is expected from the behaviour of m at higher calcium concentrations.3. At 10(-5)M this extra quantal release is more than two orders of magnitude greater than that predicted by the fourth power relationship.4. The calcium dependence of very low values of m was studied in low calcium Ringer in which the calcium was buffered by either citrate or EDTA. It was found that in the fourth power dependence of m on the external calcium concentration changes rather suddenly to an approximately linear dependence.5. The inclusion of small concentrations of cobalt in the Ringer was found to reduce m to very low values even in the presence of millimolar concentrations of calcium.6. The fourth power dependence of m on the external calcium concentration at high values of m was unaffected by the presence of cobalt. At low quantal contents the transition to a linear dependence on external calcium was again seen, but was shifted to calcium concentrations that did not require buffering.7. The fourth power to linear transition is discussed in terms of its relevance to the relationship between m and the m.e.p.p. frequency.


Age-dependent changes in the regulation of glycerolphosphate dehydrogenase in the rat brain and in a glial cell line.


The detection of chemical mutagens using the L5178y-Asn-murine leukemia in vitro and in  a host-mediated assay.


Acrodermatitis enteropathica. A clinical and biochemical survey.


The determination of 2'-O-methylnucleosides in RNA.


Triadic synaptic arrangements and their possible significance in the lateral geniculate nucleus of the monkey.


Sex differences in the elongation of the cranial base during pubescence.


The differential diagnosis of neonatal hepatitis and biliary atresia.


A modification in the response of human astrocytes to visna virus.


Two distinct types of secretory granules in an islet cell carcinoma associated with the Zollinger-Ellison syndrome.


Two distinct toxic proteins in Corynebacterium ulcerans.


Pseudohomozygous type II hyperlipoproteinemia.


Pseudomonas aeruginosa endocarditis in drug addicts.


Three cases of hepatoma.

Three cases of hepatoma.


Acute effects of alpha-methyldopa on mean blood pressure and plasma renin activity.


Three comparative techniques on tubo-plasty.


Trace elements in the human fallopian tube epithelium. Copper, zinc, manganese and potassium in the menstrual cycle. A study was undertaken to determine the amounts of certain trace elements in the normal endosalpinx and their possible variation in the normal menstrual cycle.  Atomic absorption spectrophotometry was used to estimate the levels of copper, zinc, manganese, and potassium in 23 oviduct specimens which were divided into 4 groups according to the phase of the cycle: 1) early proliferative (EP), Cycle  Days 6-10; 2) late proliferative (LP), Cycle Days 11-14; 3) early secretory (ESY, Cycle Days 16-22; and 4) late secretory (LS), Cycle Days 23-28.  A biopsy was taken from the mucosa of the intramural part, isthmus, and ampulla in each tube.  Findings indicated no differences in copper, manganese, and potassium concentrations among the different parts of the tube in each phase of the cycle.  The zinc concentration was lower (p less than .05) in the ampulla (36.9 mcg/gm dry weight) than in the isthmus (57.4) and intramural part (69) in EP, but not in the other 3 phases.  In the 4 phases of the menstrual cycle there was no significant difference between the levels of zinc and manganese, whereas the potassium concentration was highest in ES (p less than .05) and the copper concentration was highest in LS (p  less than .05).


House fly and Drosophila cell cultures as hosts for human enteroviruses.


Hemodynamic effects of acute and prolonged beta-adrenergic blockade in essential hypertension.


Acute leukemia-associated antigens.

Acute leukemia-associated antigens.


Increased activity of a factor ("transferangiotensin") favoring the binding of angiotensin II to arterial wall in hypertension.


Force-feeding effects on growth, carcass and blood composition in the young chick.


Decreased response of plasma renin activity to orthostasis in diabetic patients with orthostatic hypotension.


Increased transcapillary escape rate of albumin, IgG, and IgM during angiotensin-II-induced hypertension in man.


Connection between the structural organization of the aortic baroreceptor zone and characteristics of the baroreceptors.


Soiled disposable diapers: a potential source of viruses.


Proceedings: A role for noradrenaline and cyclic AMP in prostaglandin E1 Fever.


Proceedings: Assay of steroid hormone receptor in cancers, methodology and perspectives.


Proceedings: Clinical value of predictive tests for advanced breast cancer. With different endocrine measures remissions may be brought about in 30% of cases of advanced breast cancer.  Remissions seen after ablative procedures are best.  However, average survival of the 70% of nonresponders is short, especially if the therapy is adrenalectomy.  Several techniques to predict response have been investigated.  There is correlation between uptake of tritiated estradiol by the tumor tissue in vitro and the response to endocrinetherapy, but this test is not for routine use.  Correlation between the presence of receptors in breast cancer tissue and a favorable response to adrenalectomy or other endocrine treatments has been demonstrated.  As a first treatment nafoxidine can produce remissions but it prevents effectiveness of a second therapy with estrogens.  A successful first therapy with estrogens may be followed by a second remission with nafoxidine.  In 3 patients estrogen receptors have been negative in new biopsy material after hormone treatment although negative results had been reported in previous tests.  In these cases subjective remissions had been obtained with estrogens or androgens.


Proceedings: Correlation of serum levels of hepatoma D23 antigen, antibody and immune complexes with growth of transplanted tumours.


Proceedings: Effect of alpha-bungarotoxin and dendroaspis neurotoxins on acetylcholine responses of snail neurones.


Proceedings: Effects of angiotensin on fluid transport by rat jejunum in vivo.


Proceedings: Further evidence that secretin stimulates pancreatic secretion through adenosine 3,'5'-monophosphate (cyclic AMP).


Proceedings: Oestrogen and androgen receptors in human breast cancer.


Proceedings: Oestrogen receptors in human breast cancer.


Proceedings: Pathways for bulk outflow of aqueous humour and cerebrospinal fluid.


Proceedings: Plasma cyclic AMP response to glucagon in thyroid disease.


Proceedings: Radioimmunoassay of HCG, alpha and beta subunits in cancer.


Proceedings: Possible role of mitochondria in transmitter release.


Proceedings: The use of tissue culture in the screening of hormone sensitivity of endometrial carcinoma.


The effect of acetyl-coenzyme A on phosphate-activated glutaminase from pig kidney and brain. Phosphate-activated glutaminase (EC 3.5.1.2; l-glutamine amidohydrolase) purified from pig kidney and brain is activated by CoA and short-chain acyl-CoA derivatives. Acetyl-CoA is the most powerful activator (K(A) about 0.2mm). Acetyl-CoA is maximally effective in the absence of other activating anions such as phosphate and citrate, and at low glutamine concentrations. The negative co-operative substrate activation observed at pH7 becomes more pronounced in the presence of acetyl-CoA. Similarly to phosphate, acetyl-CoA produces at high protein concentrations a different type of activation, which is time-dependent, depends on protein concentration and is accompanied by an increase in the sedimentation coefficient. Acetyl-CoA, phosphate and citrate appear to have binding sites in common. No significant difference was observed between kidney and brain phosphate-activated glutaminase.


The effect of antiestrogens on the nuclear binding of the estrogen receptor.


The effect of repeated vaccination in an enzootic foot-and-mouth disease area on the incidence of virus carrier cattle. A comparison was made of the incidence of foot-and-mouth disease virus ;carrier' cattle in an unvaccinated enzootic area and an area where routine 6-monthly vaccination with an inactivated vaccine had been carried out for 3-4 years. The incidence of carriers in the vaccinated area was 0.49% as compared to 3.34% in the non-vaccinated area. The results indicate that, provided the immune status of the vaccinated herd is maintained at a level sufficient to prevent outbreaks of clinical disease and the re-introduction of virus is prevented through livestock movement controls, it should be possible to eradicate the disease from an enzootic area through vaccination.


The effect of sulfhydryl inhibitors on substrate oxidation and proline transport with membrane preparations from Mycobacterium phlei.


The effect of the hypocholesterolemic drug clofibrate on liver mitochondrial biogenesis. A role for neutral mitochondrial proteases.


The effect of 3',5'-adenosine monophosphate on granulocyte adhesion. Human granulocyte adhesion to glass capillary tubes was tested in the presence of agents that increase intracellular levels of cyclic 3',5'-adenosine monophosphate (cAMP). Adhesion was significantly reduced by 10(-3)-10(-4) M dibutyryl cAMP, 10(-4)-10(-6) M prostaglandin E(1) (PGE(1)), 10(-4)-10(-6) M histamine, or 10(-3) M theophylline. Adhesion was not suppressed by 10(-4) M theophylline unless it was combined with PGE(1) or histamine. Eosinophil and basophil adhesion was especially sensitive to suppression by the above agents. These findings suggest that intracellular cAMP may play a role in regulation of adhesiveness of human basophils, eosinophils, and neutrophils.


The effects of a chronic limitation of sleep length.


The effects of prolonged physiologic estradiol therapy on the maturation of hypogonadal teen-agers.


Biodegradative L-threonine deaminase of Salmonella typhimurium. The threonine deaminase formed under anaerobic conditions by Salmonella typhimurium is induced by l-serine and l-threonine, is catabolite repressible, requires cyclic adenosine 3',5'-monophosphate for its synthesis and adenylic acid for optimal activity, and is immunologically different from biosynthetic threonine deaminase.


Protein kinase activity of bovine thyroid microsomes.


Protein kinase and cyclic 3',5'-AMP: significance of binding and activation constants.


Protein kinase in the sea urchin egg cortices. Its purification and characterization and some properties of an endogenous protein kinase in the cortices.


Protein model refinement based on x-ray data.


Protein secretion in the rat ventral prostate and the relation of golgi vesicles, cisternae and vacuoles, as studied by electron microscope radioautography.


Epstein-Barr virus and human malignancies.


Epstein-Barr virus from P3HR-1 cells grown in chemically defined medium. A chemically defined medium, designated FCRC-1, supports the growth of P3HR-1 cells and concomitant replication of infectious Epstein-Barr virus.


One-electron reactions in biochemical systems as studied by pulse radiolysis. VI. Stages in the reduction of ferricytochrome c.


Caffeine inhibition of dark-repair: lack of reversal by adenosine-(3'5')-monophosphate.


The electron transport system in the cochlear hair cell: the ultrastructural cytochemistry of respiratory enzymes in hair cell mitochondria of the guinea pig.


Subcellular distribution of transmitter-related enzyme activities in discrete areas of the rat dentate gyrus.


The electrophoretic properties of some human blood cells. Effect of pH, ionic strength and antilymphocyte globulin.


Subcellular fractionation of adipocytes.


Evidence for the existence of a fully reduced state of molybdoferredoxin during the functioning of nitrogenase, and the order of electron transfer from reduced ferredoxin.


Evidence against the presence of cyclic AMP and related enzymes in selected strains of Bacteroides fragilis.


Evidence for the formation of a gamma-phosphorylated glutamyl residue in the Escherichia coli acetate kinase reaction.


Evidence for the modification of fructose 1,6-bisphosphatase by two distinct lysosomal proteases.


Epidemiology of malignant neoplasms in children and juveniles in Warsaw Province between 1966-1970.


Biogenic amines in cultured neuroblastoma and astrocytoma cells. The presence of biogenic amines in cultured cells of mouse neuroblastoma C-1300 (clone NB-2a) was suggested by fluorescence-microscope histochemistry. Incubation in media containing L-[(14)C]tyrosine and L-[(14)C]tryptophan for 24 h, followed by high-voltage electrophoresis, radiochromatogram scanning, and scintillation counting, confirmed the presence of [(14)C]dopamine, [(14)C]norepinephrine, [(14)C]epinephrine, [(14)C]serotonin, [(14)C]tyramine, and [(14)C]octopamine. Dopamine, norepinephrine, epinephrine, and serotonin were demonstrated spectrophotofluorometrically in concentrations, expressed as micrograms amine per milligram protein, of 1.19, 0.027, 0.038, and 0.148, respectively, for cells in a stationary growth phase. Fluorescence-microscope histochemistry also suggested the presence of biogenic amines in cultured astrocytoma cells (cell line C6). Spectrophotofluorometric assay of cells in a stationary growth phase demonstrated intracellular dopamine, norepinephrine, epinephrine, and serotonin in concentrations significantly lower than those of neuroblastoma cells.


Content of adenosine 3'-5'-cyclic monophosphate in the pancreatic islets of mice with a hereditary defect of insulin secretion.


Segmental and lobar atrophy of the liver.


Carpenter syndrome--acrocephalopolysyndactyly type II.


Sequential renal lipid changes in weanling rats fed a choline-deficient diet.


Concentration of enteric viruses from water with lettuce extract. A method for recovering enteroviruses, adenovirus, and reovirus from water with lettuce extract is described. Lettuce extract at pH 8.5 was added to the sample and the pH was reduced stepwise with hydrochloric acid to 4.0 to 4.5. The flocculent lettuce-extract particles, and adsorbed virus, were readily removed from solution by low-speed centrifugation. Electron microscopy suggests that, under conditions suitable for adsorption, virus particles are coated with the lettuce-extract colloid.


The enzymology of short-chain fatty acyl-coenzyme A synthetase from seeds of Pinus radiata. Kinetic studies and a proposed reaction mechanism. 1. Short-chain fatty acyl-CoA synthetase from seeds of Pinus radiata was examined by acetate- and propionate-dependent PP(i)-ATP exchange. Reaction mixtures came to equilibrium almost instantly as judged by rates of exchange and analysis of an incubation mixture. 2. The activity of the enzyme was correlated with the concentration of MgP(2)O(7) (2-) but not with the concentration of Mg(2+), as judged by PP(i)-ATP exchange and fatty acyl AMP-dependent synthesis of ATP in the presence of PP(i). In PP(i)-ATP exchange assays, no clear relationship between activity and any single species of ATP was apparent. 3. High concentrations of fatty acid inhibited PP(i)-ATP exchange. PP(i)-dATP exchange was less than PP(i)-ATP exchange at low concentrations of fatty acid, but at higher concentrations PP(i)-dATP exchange exceeded PP(i)-ATP exchange. The rate of synthesis of fatty acyl-CoA in the presence of dATP was less than with ATP. 4. ATP and propionate inhibited the synthesis of ATP from propionyl-AMP and PP(i). The inhibition by ATP was competitive with respect to propionyl-AMP and non-competitive with respect to PP(i). The inhibition by propionate was non-competitive with respect to propionyl-AMP and PP(i). 5. AMP was a competitive inhibitor of propionyl-AMP-dependent synthesis of ATP and competitively inhibited propionate-dependent PP(i)-ATP exchange when ATP was the variable substrate. 6. It was concluded that the first partial reaction catalysed by the enzyme is ordered; ATP is the first substrate to react with the enzyme and PP(i) is probably the only product released.


Stereospecific (3H)(minus)-alprenolol binding sites, beta-adrenergic receptors and adenylate cyclase.


Stereotactic anterior cingulate lesions for persistent pain: a report on 68 cases.


Proteolytic processing in the biosynthesis of insulin and other proteins.


Susceptibility of human gingival cells to some animal viruses.


Nucleotide sequence of tRNA Cys from baker's yeast.


Cancer in immunosuppressed experimental animals and human patients.


Cancer risk in ulcerative colitis: its independence of luminal factors.


Cancer- and organ-specificity of the carcinoembryonic antigen.


Letter: Accurate quantitative estimation of lactate in whole blood.


Letter: Cyclic AMP and cyclic GMP in gastric acid secretion.


Letter: A newly recognized inherited syndrome of dwarfism, craniosynostosis, retinitis pigmentosa, and multiple congenital malformations.


Letter: Hematologic and biochemical profiles in normal Demoiselle cranes.


Letter: Cytomegalovirus and penta X syndrome.


Letter: Interference by antibiotics in amino acid assays.


Letter: Lead and intelligence.

Letter: Lead and intelligence.


Letter: Nephroblastoma--genetic aspects.


Letter: Nephrotic syndrome with oat-cell carcinoma.


Letter: Prostaglandins in renal venous blood of essential hypertensive patients.


Letter: Regulation of aldosterone biosynthesis by "angiotensin III".


Letter: The dynamic voltage-transfer function for rod-bipolar cell transmission.


Letter: Transmission of feline leukemia.


Letter: Zoster in three children in family.


Cooperativity in the inhibition of oxidative phosphorylation by chlorophenoxyisobutyrate.


Temperature-sensitive DNA polymerase from Rous sarcoma virus mutants.


Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of group I and group IV mutants. When tested in vitro, certain temperature-sensitive (ts) mutants of vesicular stomatitis virus (VSV) belonging to complementation groups I and IV appear to have defects in the virion-bound polymerase. To obtain further information concerning the nature of these defects, representative mutants were dissociated by the method of S. Emerson and R. Wagner (1972), and their supernatant (S) and pellet (P) fractions were tested for transcriptase activity when combined with the P and S fractions, respectively, of VSV-HR virions. It was found that the S fractions from group I mutants tsW4, 11, 14, 15, and 28 were defective in transcriptase activity, whereas their P fractions were as active as those of VSV-HR. On the other hand, the P fraction derived from virions of the group IV mutant tsW16B showed reduced activity at 25 C and very little activity at 38 C. These results suggest that our group I mutants, like those examined by D. Hunt and R. Wagner (1974), have a defect in the soluble transcriptase enzyme, whereas mutant tsW16B (group IV) has a defect in a sedimentable component required for transcriptase activity, possibly in the ribonucleoprotein template.


Differences in choroid plexus concentration of pertechnetate produced by varying time of pertechnetate administration and brain imaging.


Different types of mutagenic effect of ethylmethanesulfonate on Drosophila melanogaster.


Differential activation of biologically distinguishable endogenous mouse type C RNA viruses: interaction with host cell regulatory factors.


Differential diagnosis of tumors of the salivary glands with radioactive isotopes.


Bacterial exoenzymes toxic for insects; proteinase and lecithinase.


Bacterial interference between strains of S. aureus.


Epidermolysis in a case of severe cytomegalovirus infection.


Concerning specificity of the influence of pentoxifylline on various cyclic AMP phosphodiesterases.


Pattern of osmium deposition in the parietal cells of the stomach. Parietal cells of the stomach of the hamster show extensive amounts of dense material in a variety of organelles after prolonged exposure to a solution of osmium tetroxide. Conspicuous amounts of reduced osmium compounds are evident within the granular endoplasmic reticulum, perinuclear cisterna, and vesicular elements of the Golgi complex. Dense material is also apparent within cristae of the mitochondria, the surface coat of the microvilli of the intracellular canaliculus, and vesicular elements of the multivesicular bodies. Multivesicular bodies, containing numerous small osmiophilic elements, are often seen surrounding and/or in close contact with mitochondria. The proximity of the multivesicular bodies to the mitochondria appears to be related to an autophagic process involving degradation of mitochondria. The distribution and intensity of the precipitates within the organelles of the parietal cells vary in different regions of the gastric glands. The findings of this study emphasize that cell structures other than the Golgi complex may contain large concentrations of reduced osmium compounds after prolonged exposure to a solution of osmium tetroxide.


Dispersion of central nervous system tumors. Correlation between clinical aspects and tissue culture studies.


Properties of a phosphoprotein phosphatase from bovine heart with activity on glycogen synthase, phosphorylase, and histone.


Properties and substrate specificity of some reactions catalysed by a short-chain fatty acyl-coenzyme A synthetase from seeds of Pinus radiata. 1. Crude extracts of seeds of Pinus radiata catalysed acetate-, propionate-, n-butyrate- and n-valerate-dependent PP(i)-ATP exchange in the presence of MgCl(2), which was apparently due to a single enzyme. Propionate was the preferred substrate. Crude extracts did not catalyse medium-chain or long-chain fatty acid-dependent exchange. 2. Ungerminated dry seeds contained short-chain fatty acyl-CoA synthetase activity. The activity per seed was approximately constant for 11 days after imbibition and then declined. The enzyme was located only in the female gametophyte tissue. 3. The synthetase was purified 70-fold. 4. Some properties of the enzyme were studied by [(32)P]PP(i)-ATP exchange. K(m) values for acetate, propionate, n-butyrate and n-valerate were 4.7, 0.21, 0.33 and 2.1mm respectively. Competition experiments between acetate and propionate demonstrated that only one enzyme was involved and confirmed that the affinity of the enzyme for propionate was greater than that for acetate. CoA inhibited fatty acid-dependent PP(i)-ATP exchange. The enzyme catalysed fatty acid-dependent [(32)P]PP(i)-dATP exchange. 5. The enzyme also catalysed the fatty acyl-AMP-dependent synthesis of [(32)P]ATP from [(32)P]PP(i). Apparent K(m) (acetyl-AMP) and apparent K(m) (propionyl-AMP) were 57mum and 7.5mum respectively. The reaction was inhibited by AMP and CoA. 6. Purified enzyme catalysed the synthesis of acetyl-CoA and propionyl-CoA. Apparent K(m) (acetate) and apparent K(m) (propionate) were 16mm and 7.5mm respectively. The rate of formation of acetyl-CoA was enhanced by pyrophosphatase. 7. It was concluded that fatty acyl adenylates are intermediates in the formation of the corresponding fatty acyl-CoA.


Properties of 3-hydroxy-3-methylglutaryl coenzyme A reductase solubilized from rat liver and hepatoma.


Herpes encephalitis therapeutic considerations.


Herpes encephalitis with catatonic stupor.


Glomerular beta-lipoprotein in childhood renal diseases.


Herpes genitalis and aseptic meningitis.


Herpes simplex lesions of the skin diagnosed by the immunofluorescence technique.


Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. II. An ultrastructural study of viral penetration. An electron microscope study was carried out on the early minutes of herpes simplex virus (HSV) and cytomegalovirus (CMV) penetration into WI-38 cells. Both HSV and CMV entered cells either by fusion of the viral envelope with a limiting cell membrane, or via phagocytosis. Both fusion and phagocytosis occurred within 3 min after the initiation of penetration. After fusion, the naked capsids of CMV free in the cytoplasm became coated with a fine, fibrillar material. CMV capsids thus coated retained a well-defined and easily identifiable morphology until the eclipse of visible viral particles between 1 and 1.5 days postinfection. In contrast, naked HSV capsids free in the cytoplasm were never coated. Rather, within minutes after penetration, they assumed a rounded, less regular outline, and were no longer detectable by 90 to 120 min postinfection. The free naked capsids of both viruses appeared to migrate across the cytoplasm toward the nucleus and to become located near nuclear pores. Both HSV and CMV capsids reached the nucleus as early as 5 min after the initiation of penetration. No further interaction with the nucleus could be documented. Particles were also consistently identified in the Golgi region. Phagocytosed particles generally remained within phagosomes, where they appeared to be degraded. However, stages were identified in what is believed to be the escape of enveloped viruses from phagosomes into the cytoplasm via fusion of their envelope with the phagosomal membrane.


Herpes virus latency in spinal ganglia of mice without illness.


Herpes viruses and oncogenesis.

Herpes viruses and oncogenesis.


Successful extraction of large intracaval isthmus of nephroblastoma.


Herpes genitalis and its relationship to cervical cancer.


Depression of cell-mediated immunity during acute infectious mononucleosis.


Depression of human monocyte chemotaxis by herpes simplex and influenza viruses.


Expression of purified mitochondrial poly(A)polymerase of hepatomas by an endogenous primer from liver..


Progesterone and androgen receptors in non-flagellate germ cells of the rat testis.


Nondestructive separation of rat liver rough microsomes into ribosomal and membranous components.


Herpesvirus encephalitis and pulmonary sarcoidosis. A case report with necropsy findings.


Cholesteryl ester metabolism in tissue culture cells. I. Accumulation in Fu5AH rat hepatoma cells.


Competitive protein-binding radioassay of urinary cortisol.


Pasteur effect and phosphofructokinase.

Pasteur effect and phosphofructokinase.


EEG evaluation of a sleep recorder.

EEG evaluation of a sleep recorder.


Purification and properties of an extracellular acid ribonuclease from Penicillium janthinellum.


Modification of diphtheria toxin by NAD.


Purification and regulatory properties of fructose 1,6-diphosphatase from Hydrogenomonas eutropha. Fructose diphosphatase of Hydrogenomonas eutropha H 16, produced during autotrophic growth, was purified 247-fold from extracts of cells. The molecular weight of the enzyme was estimated to be 170,000. The enzyme showed a pH optimum of 8.5 in both crude extracts and purified preparation. The shape of the pH curve was not changed in the presence of ethylenediaminetetraacetic acid. The enzyme required Mg(2+) for activity. The MgCl(2) saturation curve was sigmoidal and the degree of positive cooperativity increased at lower fructose diphosphate concentrations. Mn(2+) can replace Mg(2+), but maximal activity was lower than that observed with Mg(2+) and the optimal concentration range was narrow. The fructose diphosphate curve was also sigmoidal. The purified enzyme also hydrolyzed sedoheptulose diphosphate but at a much lower rate than fructose diphosphate. The enzyme was not inhibited by adenosine 5'-monophosphate but was inhibited by ribulose 5-phosphate and adenosine 5'-triphosphate. Adenosine 5'-triphosphate did not affect the degree of cooperativity among the sites for fructose diphosphate. The inhibition by adenosine 5'-triphosphate was mixed and by ribulose 5-phosphate was noncompetitive. An attempt was made to correlate the properties of fructose diphosphatase from H. eutropha with its physiological role during autotrophic growth.


The fine structure of the ovarian follicle of Alloteuthis subulata Lam. (Mollusca, Cephalopoda).


The fine structure of the lutein cells in the blue fox (Alopex lagopus) with special reference to the secretory activity during pregnancy.


The foot-and-mouth disease virus subtype variants in Kenya. The subtype variants found in Kenya in the past ten years have been studied. The type O and type SAT 2 subtypes have a distinct geographical distribution which appears to be associated with livestock movement patterns. The type A viruses have a greater tendency to antigenic variation and their geographical distribution is less distinct. In type C only minor differences exist between the three viruses studied.


Wakefulness and REM sleep in human neonates.


The eye and its disorders. 29. Diseases of the visual pathways.


Rat gastric mucosal cAMP following cholinergic and histamine stimulation.


Cytogenetic study of primary tumors induced in the golden hamster by chemical and viral agents. Communication II. Karyotypic characteristics of tumors induced by the simian virus 40 (SV40) and the combined action of virus and 7,12-dimethylbenzanthracene.


The general febrile respiratory form of infectious bovine rhinotracheitis in calves.


The flower pot technique of rapid eye movement (REM) sleep deprivation.


Antigenic makeup of subcellular fractions of Trypanosoma cruzi.


Changes during development of mouse brain in the activities and subcellular distributions of creatine and adenylate kinases.


Changes in intra-ovarian pressure in woman following the administration of luteinizing hormone-releasing hormone LH-FSH).


Hypoglycemia: pathogenesis, diagnosis and treatment.


Recognition by electrophoretic localisation of the extracellular products of selected strains of staphylococci.


Assignment of the gene for galactokinase to human chromosome 17 and its regional localisation to band q21-22.


Antagonism of morphine by beta-melanocyte-stimulating hormone and by tetracosactin.


Antagonism of dopamine-mediated inhibition in the nigro-striatal pathway: a mode of action of some catatonia-inducing drugs.


Antagonistic effect of methadone on steroidogenesis and the adenylate cyclase system in isolated rat adrenocortical cells.


Lymph node metastasis as first manifestation of glioblastoma. Case report.


Energy-linked reactions in photosynthetic bacteria. IX. Pi-PPi exchange in Rhodospirillum rubrum.


On the electron transfer reaction between ferricytochrome c and ferrohexacyanide in the pH range 5 to 7.


Is the neurotransmitter system abnormal in multiple sclerosis?


On the origin of the genes for neoplasia: G.H.A. Clowes memorial lecture.


On the extracellular synthesis of adenosine triphosphate by mammalian cells.


On the problem of the origin of nerve terminals in the sympathetic ganglia.


On the question of the primary acceptor in bacterial photosynthesis:manganese substituting for iron in reaction centers of Rhodopseudomonas spheroides R-26.


On the relationship between cell division and cyclic adenosine-3',5'-monophosphate in Escherichia coli.


On the association of some dermatoses with South American pemphigus foliaceus.


Epithelial inclusions in the vas deferens: an electron microscopic study.


On the ultrastructure of the rat anococcygeus muscle.


Biochemical and endocrine differences between normotensive and spontaneously hypertensive rats.


Further studies on postsynaptic potential in masseteric motoneuron.


Biochemical and morphological characterization of azurophil and specific granules of human neutrophilic polymorphonuclear leukocytes. Postnuclear supernates from homogenates of purified neutrophil polymorphonuclear leukocytes (PMNs) from human blood were fractionated by zonal sedimentation and isopycnic equilibration in sucrose gradients. The fractions were characterized biochemically by measuring protein content and the activities of eight enzymes. Selected fractions were further analyzed by electron microscopy. In both centrifugation systems, azurophil and specific granules could be resolved almost completely. Azurophil granules sediment three to four times faster than the specifics and have an average density of 1.23. They contain all the peroxidase of the cells, large portions of four lysosomal hydrolases, and about half of the total lysozyme, and therefore appear to be, in biochemical terms, very similar to the azurophil granules of rabbit PMNs. The specific granules, which have an average density of 1.19, contain the remaining half of the lysozyme but appear to be free of the other components of the azurophil granules, and of alkaline phosphatase. Isopycnic equilibration disclosed a minor lysosomal population, which strongly overlaps the specific granules, and made possible the identification of a membrane-fraction which is characterized by the presence of the thiol-sensitive acid 4-nitrophenyl phosphatase and of alkaline phosphatase.


Synthesis of simian virus 40 DNA in isolated nuclei.


Synthesis and secretion of fibrinogen and albumin by isolated rat hepatocytes.


Synthesis of some new semisynthetic penicillins.


Morphological and cytological effects of carbenicillin on Pseudomonas pseudomallei.


Psychological aspects of cardiac surgery. Psychiatric grand rounds.


Morphology and X-ray sensitivity of rat glial cells after cytochalasin B treatment.


Morphology of the aging brain, human and animal.


N-(phosphonacetyl)-L-aspartate, a potent transition state analog inhibitor of aspartate transcarbamylase, blocks proliferation of mammalian cells in culture.


Psychotropic drugs and some enzymes of energy metabolism.


Prophylactic use of antibiotics in preventing bovine respiratory disease complex.


Staphylococcal sphingomyelinase (beta-hemolysin).


The hyperthermic effect of intracerebroventricular cholera enterotoxin in the unanaesthetized cat. 1. Cholera enterotoxin was used to evaluate a possible role of endogenous cyclic AMP in production of hyperthermia. Injection of purified toxin (0.10-1.0 mug in 0.10 ml.) into the lateral cerebral ventricle of unanaesthetized cats caused dose-related hyperthermic responses. Heating the toxin for 40 min at 90 degrees C abolished its hyperthermic activity.2. Intraventricular administration of dibutyryl cyclic AMP (250-1000 mug) also caused hyperthermia which, however, was preceded by transient periods of hypothermia and/or excitation in about half of the tests.3. Paracetamol, indomethacin and sodium salicylate inhibited hyperthermic responses to cholera enterotoxin. Paracetamol and indomethacin also inhibited hyperthermia induced by dibutyryl cyclic AMP (sodium salicylate was not tested).4. It is likely that the hyperthermic effect of cholera enterotoxin in the cat is mediated via endogenous cyclic AMP and that the antipyretics inhibit this effect by an action subsequent to the increase in cyclic AMP.5. It is unlikely that prostaglandin-induced hyperthermia in the cat is mediated via cyclic AMP since these antipyretics do not inhibit this response to prostaglandin E(1).


Cardiac hypertrophy in spontaneously hypertensive rats.


The hypothalamo-hypophysial system of the lamprey, Lampetra fluviatilis L. I. the neuohypophysis.


A trial of corticotrophin gelatin injection in acute optic neuritis. The effect of a 30 day course of corticotrophin gelatin injections on the natural history of acute unilateral optic neuritis in 27 patients is reported. A further 27 patients received inert injections in a double-blind control. Measurements were made on visual acuity, visual field, macular threshold, and colour vision, and the development of optic atrophy and other neurological episodes was recorded. No significant differences were found between treated and control groups at intervals up to two years from the start of the trial. The criteria for inclusion of patients are given and it is concluded that corticotrophin has no effect on the natural history of the condition as defined.


Ovarian hilus-cell tumours: their varying presentations.


Ovarian neoplasms at the Chelsea Hospital.


Possible higher valence states of cytochrome P-450 during oxidative reactions.


Amebiasis in a mental institution: serologic and epidemiologic studies.


A cribriform cylindroma with metastases.


Steric and electrostatic interactions in reactions of carbohydrates. III. Direct displacement of the C-2 sulfonate of methyl 4,6-o-benzylidene-3-o-methyl-2-o-methylsulfonyl-beta-d-gluco-and-mannopyranosides.


Cyclic AMP levels in Phycomyces during a response to light.


Cyclic adenosine monophosphate-dependent phosphorylation of specific fat cell membrane proteins by an endogenous membrane-bound protein kinase. Possible involvement in the regulation of insulin-stimulated glucose transport.


Cyclic AMP mediates the concanavalin A agglutinability of mouse fibroblasts. We have devised a quantitative way to measure the agglutination of cells which utilizes the size discrimination feature of an automatic particle counter. With this method we have studied the agglutinability by concanavalin A of 3T3 cells, a mutant of 3T3 cells (3T3cAMP(tcs)) in which cyclic AMP levels fall when the cells are subjected to temperature change or fresh serum, and L929 cells. We find with 3T3cAMP(tcs) cells that low levels of cyclic AMP correlate with increased agglutinability and that high levels of cyclic AMP correlate with decreased agglutinability. Prior treatment of these cells with a cyclic AMP phosphodiesterase inhibitor or Bt(2)cAMP blocks the increase in agglutinability induced by temperature change. When 3T3 cells are treated with fresh serum, their agglutinability also increases although to a much smaller extent than with 3T3cAMP(tcs) cells. Cells change their agglutinability very rapidly. Treatment of L929 cells for 15 min with 1-methyl-3-isobutyl xanthine at 1 mM decreases their agglutinability to the level of normal 3T3 cells. We conclude that in normal and transformed cells the level of cyclic AMP regulates agglutinability.


Cyclic GMP and cyclic AMP levels in normal and transformed fibroblasts.


Surgical aspects of amebiasis.

Surgical aspects of amebiasis.


Clinical antitumor activity and toxicity of streptozotocin (NSC-85998).


[Facial paralysis of unknown origin. Testing for a viral etiology by antibody measurement].


Atypical blood lymphocytes in sarcoidosis: morphology, cytochemistry, and membrane properties.


Physical properties of a dog liver and kidney cytosol protein that binds thyroid hormone.


Physical protein regulation of adenylate kinases from muscle, liver, and hepatoma.


Atypical demyelinating disease.

Atypical demyelinating disease. A 17 year old girl died after an illness characterized by progressive mental deterioration and severe myoclonic jerks. Extensive pathological, virological, and immunological studies failed to support the diagnosis of subacute sclerosing panencephalitis but were compatible with disseminated demyelinating disease. The spinal fluid was positive for measles virus antibodies when examined by the fluorescent antibody technique, by complement fixation, and by haemagglutination inhibition tests, but the antibody titres were not high. The concentration of vaccinia antibody in the serum was consistent with that found in the general population and none was detected in the spinal fluid. Animal and tissue culture studies failed to disclose a viral agent, but pathological sections revealed perivascular cellular infiltration, demyelination, rare inclusion bodies, and multinucleated giant cells. Although these studies were not definitive, a record of procedures used and results obtained, both positive and negative, may be helpful to other investigators interested in defining more accurately the clinical features and the pathogenesis of these diseases.


Chemical structure of a modification of the Escherichia coli ribonucleic acid polymerase alpha polypeptides induced by bacteriophage T4 infection.


Clinical use of renin determinations.

Clinical use of renin determinations.


Chemical synthesis and biological activity of 3-deoxy-1alpha-hydroxyvitamin D3 an analog of 1alpha,25-(OH)2-D3, the active form of vitamin D3.


Application of a vidicon spectrometer for simultaneous flame-emission analysis for sodium and potassium in serum.


Application of immune electron microscopy to the study of the antigenic relationships between three new human papovaviruses.


Corticosteroids: a direct effect at the neuromuscular junction.


Corticotropin release during ECT in man.


Deamidation of glutaminyl and asparaginyl residues in peptides and proteins.


President's address. Myasthenia gravis.

President's address. Myasthenia gravis.


Studies of the regulation of colicin Ib synthesis. II. The synthesis of col Ib-P9 specific RNA in vivo.


Studies of serum lipoprotein concentrations in children: preliminary report.


Studies of NAD(H) conformations in solution: a new method using the PMR spectra of cobalt ion-dinucleotide complexes.


Prediction of the secondary structure of proteins using the helix-coil transition theory.


Studies on the diagnostic value of an immunofluorescence test for EB virus-specific IgM. A modification of the test for EB virus/IgM introduced by Schmitz and Scherer (1972) is described. It is simple and gives reproducible results.EB virus/IgM was demonstrated in all but one case of infectious mononucleosis and in students with minor illness shown to have acquired EB virus/IgG recently. Unlike the EB virus/IgG, the IgM disappears within a few months. Although the Paul-Bunnell-Davidsohn test is still the test of choice for the diagnosis of infectious mononucleosis, the EB virus/IgM test could be useful to establish a diagnosis of current or recent EB virus infection where the Paul-Bunnell-Davidsohn test was negative or equivocal.


Studies on the effects of acetylcholine, epinephrine, dibutyryl cyclic adenosine monophosphate, theophylline, and calcium on the synthesis of myoglobin in muscle cell cultures estimated by radioimmunoassay.


Studies on the growth and changes in metabolism of rats fed on carbohydrate-deficient fatty acid-based diets supplemented with graded levels of maize starch.


Studies on the in vitro adenylation of RNA by vesicular stomatitis virus.


Studies on the mechanism of deacylation of aminoacyl-tRNAs by aminoacyl-tRNA synthetases in the absence of adenosine monophosphate and pyrophosphate.


Studies on the stability of the hemagglutinating antibody of Entamoeba histolytica.


Studies on the inhibition of RNA synthesis by phalloidin in primary cultures of fetal cells.


Studies on the structure-activity relationships of synthetic LHRH analogs.


Studies on 3,7-dimethyl-1-(5-oxo-hexyl)-xanthine(BL 191). I. Cyclic 3',5'-nucleotide phosphodiesterase (PDE) and the inhibitory effect of BL 191 on PDE in rat brain and heart.


Specific binding of growth hormone to thymocytes.


Amplification of a circular segment of SV40 DNA.


Calcification of selected strains of Streptococcus mutans and Streptococcus sanguis. Nine strains of cariogenic Streptococcus mutans and two strains of Streptococcus sanguis were tested for their ability to form hydroxyapatite. The cells were examined by X-ray diffraction and electron microscopy for apatite crystals after growth in a synthetic calcification medium. Each of the test isolates, except for one strain of S. sanguis, produced intracellular mineral. Two strains of S. mutans formed both intra- and extracellular crystals. There was no apparent relationship between calcifiability and serotype.


Stability and enzyme separation: integral representation of the solutions.


Stability studies and tumor uptake of a technetium-tetracycline complex.


Haemin and nicotinamide adenine dinucleotide requirements of Haemophilus influenzae and Haemophilus parainfluenzae.


Reagin synthesis in inbred strains of rats.