Cellular reactions against Burkitt Lymphoma cells. I. Colony inhibition with effector cells from patients with Burkitt's lymphoma.


Compared action of a polyoma-virus-specific inhibitor on the formation of polyoma capsid antigen and of polyoma tumor antigen.


Spontaneous appearance of cytopathology and rat C-type virus (WF-1) in a rat embryo cell line.


Studies on type-H virus-like particles in hamster: their role in oncogenesis.


Cytomegalovirus infections in leukaemic children.


Cellular immunity in Wilm's tumour and neuroblastoma.


The formation of variants with a reversion of properties of transformed cells. VII. Chromosome numbers and re-reversion in subtetraploid variants.


Is the brain behind the eye? Implications of processing by the retina.


A varicella-like disease in macaque monkeys.


Failure to induce fetal infection by inoculation of pregnant immune heifers with bovine parainfluenza 3 virus.


Host defense mechanisms against herpes simplex virus. II. Protection conferred by sensitized spleen cells.


Spontaneous Herpesvirus saimiri lymphoma in an owl monkey.


Incidence of antibody to envelope antigen of herpes simplex virus type 2 among patients with cervical carcinoma and matched controls.


The effects of endogenous compounds on ovine myometrial contractility and adenyl cyclase system.


Transmission of rhinovirus colds by self-inoculation.


Inapparent congenital cytomegalovirus infection. A follow-up study.


Infant of the addicted mother.

Infant of the addicted mother.


Median-nerve neuropathy after percutaneous puncture of the brachial artery in patients receiving anticoagulants.


Congenital cytomegalovirus infection.

Congenital cytomegalovirus infection.


Isolation of human amnion cells transformed by rescuable murine sarcoma virus.


Studies on the fine structure of the protozoan Cyclidium, with special reference to the mitochondria, pellicle and surface-associated bacteria.


[Ultrastructure of the dorsal giant fibre system in the ventral nerve cord of the earthworm. I. Somata of the giant fibres].


The ultrastructure of the caecal epithelium of the intraredial cercaria of Neophasis lageniformis (Lebour, 1910) (Trematoda, Acanthocolpidae).


The synaptic ribbons of the guinea-pig pineal gland under normal and experimental conditions.


Mortality, antibody development, and viral persistence in porcine fetuses incubated in utero with SMEDI (entero-) virus.


Arrhenoblastoma: in vivo and in vitro studies.


Feline virus-induced tumors and the immune response: recent developments.


A simple method for obtaining undiluted nasal secretions from cattle.


Vaccination against Marek's disease: possible causes of failure of herpesvirus of turkeys (strain FC126) to protect chickens against Marek's disease.


Abdominal-muscle rigidity induced by morphine and nitrous oxide.


The mitotic apparatus of a zygomycete, Phycomyces blakesleeanus.


Comparison of early injury to liver endoplasmic reticulum by halomethanes, hexachloroethane, benzene, toluene, bromobenzene, ethionine, thioacetamide and dimethylnitrosamine.


Cell wall composition and synthesis via Golgi-directed scale formation in the marine eucaryote, Schizochytrium aggregatum, with a note on Thraustochytrium sp.


Effect of cyclic 3',5'-monophosphate on edema and granuloma induced by carrageenin.


Effect of protein deficiency on the inducibility of the hepatic microsomal drug-metabolizng enzyme system. II. Effect of enzyme kinetics and electron transport system.


Inhibition of some mitochondrial functions by acrolein and methylvinylketone.


Inhibition by cysteamine of steroid 11  -hydroxylation.


Importance of the route of administration of ccl 4 in the protective effect of promethazine.


Magnetic resonance studies on membrane and model membrane systems. 3. Fatty acid motions in aqueous lecithin dispersions.


Effect of proteins on the motion of spin-labeled fatty acids in mycoplasma membranes.


Preparation of plasma membranes from isolated cells of newborn rat brain.


Platelet dysfunction in glycogen storage disease type I.


Neuroradiology of juxtasellar mass lesions.


Heterogeneity of acid phosphatase activity in human polymorphonuclear leukocytes.


Communication for child mental health: 1973 NIMH information interchange.


The fate of mestranol (17 -ethynylestradiol-3-methyl ether) in the isolated perfused rat liver.


Effect of insulin on cerebral adenyl cyclase and phosphodiesterase.


The influence of deamidation on the biological activity of porcine adrenocorticotropic hormone (ACTH).


Inhibitors of proteolysis and glucose uptake by diaphragm muscle in vitro under insulin action.


Further studies with precipitation in gel test in diagnosis of smallpox. I. Studies on detection of antibodies in sera by pig test.


Aflatoxin hepatic carcinogenesis: an experimental comparative study.


Comparative drug trials in symptomatic and asymptomatic non-dysenteric amoebic colitis.


Minor salivary gland tumors: a survey of 64 cases.


Malignant mixed tumor of parotid gland with a solitary metastasis to the radius.


Aminoacyl-tRNA synthetase activity in virions of avian myeloblastosis virus.


Transfer of the B77 virus genome from virogenic mammalian cells to other mammalian cells by cell fusion.


Monoclonal macroglobulinemia and cytomegalic inclusion disease.


Electron microscopic demonstration of adenovirus in appendix vermiformis in a case of ileocecal intussusception.


Cancer of the colon and disseminated intravascular coagulation.


A sensitive cell culture system for the virus of porcine inclusion body rhinitis (cytomegalic inclusion disease).


Tests in sheep of attenuated sheep pox vaccines.


Factors associated with the production of attenuated sheep pox vaccines.


Antagonists of angiotensin. Effect on the blood pressure of normal and hypertensive rats.


Presence and stimulation of adenyl cyclase in pancreas homogenate.


Generalized cytomegalic inclusion disease presenting an infectious mononucleosis syndrome (so-called cytomegalovirus mononucleosis) in a previously healthy adult--an autopsy study.


Pathological studies on viral pneumonia and interstitial pneumonitis caused by unknown etiology.


Post-traumatic hypothalamic obesity--an autopsy case.


Role of the small intestine in cholesterol metabolism.


Lipoproteins and the relative importance of plasma cholesterol and triglycerides in peripheral arterial disease.


CNS: afferent mechanisms with emphasis on physiological and behavioral correlations.


Hypothalamic control of adenohypophysial secretions.


Biochemical physiology of central synapses.


Wakefulness and feeding in human newborns.


Repeated oral ingestion of 5-hydroxytryptophan. The effect on behavior and sleep processes in two schizophrenic children.


Indetification and characterization of pulmonary dust burden in pneumoconiosis.


Biochemical studies of liver tumors of children.


Separation of plasma lipids by thin-layer chromatography.


Congenital mesoblastic nephroma and its recurrence.


Hexachlorobenzene and rat liver.

Hexachlorobenzene and rat liver.


Grouped muscle action potential area.

Grouped muscle action potential area.


Peripheral nerve damage resulting from local hemorrhage and ischemia.


Rat mammary gland nuclear RNA polymerases in late pregnancy and lactation.


Interaction of 6-thioguanylic acid homologues with ribonuclease T 1 . Spectrophotometric and gel filtration studies.


Degradation of ribosomal RNA during chicken lens development.


Lack of significant visible C-type virus activation in the bone marrow and thymus of mice given a leukaemogenic dose of methylnitrosourea.


Tracheotomy in infants and children.

Tracheotomy in infants and children.


Antibodies to viral and synthetic double-stranded RNA in discoid lupus erythematosus.


Fine structural analysis of rabbit synovial cells. I. The normal synovium and changes in organ culture.


Effects of ethanol on afferent transmission in the central visual pathway of cats.


Partial reduction of ferricytochrome c by cyanide mediated by disulphide compounds.


Iron binding in human liver ferritin.

Iron binding in human liver ferritin.


Origin of the milk fat globule.

Origin of the milk fat globule.


The requirement of membrane sialic acid in the stimulation of phagocytosis by the natural tetrapeptide, tuftsin.


The role of acidic phospholipids in glucagon action on rat liver adenylate cyclase.


Hormonal regulation of adipocyte enzymes. The effects of epinephrine and insulin on the control of lipase, phosphorylase kinase, phosphorylase, and glycogen synthase.


Glucocorticoid receptors in lung. I. Specific binding of glucocorticoids to cytoplasmic components of rabbit fetal lung.


The enzymatic cleavage of 5'-triphosphate termini from ribonucleic acid polymerase products.


The structure of oxidized cytochrome c 2  of Rhodospirillum rubrum.


Complex formation between bacitracin peptides and isoprenyl pyrophosphates. The specificity of lipid-peptide interactions.


Studies on enzymes involved in the catabolism of phospholipids in Escherichia coli.


Studies on rat liver phenylalanyl transfer ribonucleic acid synthetase. II. Further purification, substrate specificity, and effects of substrates on heat inactivation.


Novel phosphoglucans from the cytoplasm of Phytophthora palmivora and their selective occurrence in certain life cycle stages.


Purification and properties of Ascaris cytochrome b 560.


Leukocytic response to adrenocorticotrophic hormone as influenced by the infectious history of the mammary gland.


Effects of stress on serum zinc and plasma corticoids in dairy cattle.


Reduction of iodine transfer to milk of cows after perchlorate ingestion.


Primary pulmonary neoplasms in beagle dogs exposed to aerosols of  144 Ce in fused-clay particles.


Correlation between tumor invasiveness and content of A-type particles in Ehrlich ascites tumor: a model of quantitative electron microscopy.


Epidemiology of cancer of the pancreas.

Epidemiology of cancer of the pancreas.


Mammary tumor virus (MTV) infection of CBA/Bln mice involving production of antibodies to MTV.


Spontaneous tumors of aging wild house mice. Incidence, pathology, and C-type virus expression.


Histoproliferative effect of Rauscher leukemia virus on lymphatic tissue. IV. Lactic dehydrogenase virus and erythropoietin potentiation of the eythroid response.


Increased oncogenicity and synthesis of herpesvirus antigens in hamster cells exposed to herpes simplex type-2 virus.


Oncogenicity of plaque variants of CELO virus in newborn hamsters.


Suppression of growth of P-815 mastocytoma cells in vitro by drugs increasing cellular cyclic 3',5'-adenosine monophosphate.


Soluble and membrane-bound adenylate cyclase activity in Yoshida ascites hepatoma.


Ketone body oxidation by mouse hepatoma BW7756.


Detailed hormonal studies during and after pregnancy in a previously hypophysectomized patient.


Corticotrophin-releasing factor activity in hypothalamic extracts of man.


Iodide perchlorate discharge test in lithium-treated patients.


Bioassay of calcitonin by determinations of blood calcium-45 levels in rats and mice.


Remitting signs in cerebral mass lesions.


Studies in children with impetigo. Bacteriology, serology, and incidence of glomerulonephritis.


Guillain-Barré syndrome. Occurrence following combined mumps-rubella vaccine.


Hereditary recurrent brachial neuropathy.


Decrease in HCG--binding capacity of target tissues influenced by hypophysectomy.


Vascular damage and lipid peroxidation in choline-deficient rats.


The phosphorylases system of the cardiac muscle of normal and reserpinized rats poisoned with 2,4-dinitrophenol.


Alterations in lipid metabolism following trauma in rabbits.


Distribution of a renin-like enzyme in the bovine adrenal gland.


pH-dependent nuclear magnetic resonance spectra of  15 N-enriched glycine. Line shape and relaxation studies.


The reaction of Ca ++ with the inner and outer membrane of mitochondria.


Crystal and molecular structure of the salt (1-methylnicotinamide)(+) aden-9-ylacetate(-) dihydrate. A model for the intramolecular interactions of oxidized nicotinamide adenine dinucleotide.


Synthesis of 6'-cyano-6'-deoxyhomoadenosine-6'-phosphonic acid and its phosphoryl and pyrophosphoryl anhydrides and studies of their interactions with adenine nucleotide utilizing enzymes.


Antibody affinity chromatography of hog and bovine spleen DNase II.


Analysis of corticosteroids in mixtures by gradient elution liquid chromatography.


The action of streptolysin S on mouse-liver mitochondria.


Differential responses of crab neuromuscular synapses to cesium ion. Excitatory postsynaptic potentials (EPSP's) generated in crab muscle fibers by a single motor axon, differ in amplitude and facilitation. Some EPSP's are large at low frequencies of stimulation and show little facilitation; others are smaller and show pronounced facilitation. When K(+) is replaced by Cs(+) in the physiological solution, all EPSP's increase in amplitude, but small EPSP's increase proportionately more than large ones. Quantal content of transmission, determined by external recording at single synaptic regions, undergoes a much larger increase at facilitating synapses. The increase in quantal content of transmission is attributable to prolongation of the nerve terminal action potential in Cs(+). After 1-2 h of Cs(+) treatment, defacilitation of synaptic potentials occurs at synapses which initially showed facilitation. This indicates that Cs(+) treatment drastically increases the fraction of the "immediately available" transmitter store released by each nerve impulse, especially at terminals with facilitating synapses. It is proposed that facilitating synapses normally release less of the "immediately available" store of transmitter than poorly facilitating synapses. Possible reasons for this difference in performance are discussed.


Prevalence of cytomagalovirus antibodies in the population of Northern Greece.


Functional changes in motoneurones of hemiparetic patients. Forty-six patients have been studied after upper motor neurone lesions of cerebrovascular origin. The numbers of functioning motor units in extensor digitorum brevis muscles were reduced to approximately half between the second and sixth months after a hemiplegic episode. The surviving motor units tended to have slow twitches and appeared to increase their sizes after the lesions had been present for about 20 months. The findings are explained on the basis of transsynaptic changes in alpha-motoneurones after degeneration of corticospinal fibres.


Neuromuscular block after intra-arterially injected acetylcholine. 1. Introduction, methods, and technique. The neuromuscular depolarizing block induced by intra-arterially injected ACh was studied to determine the variability in the same subject and in different subjects without disorders at the motor end-plate. Amplitude of action potentials of the opponens pollicis muscle evoked by intermittent repetitive supramaximal stimulation of the median nerve at the wrist were recorded for one hour from the beginning of ACh injection. The features of prompt and late depression stages after the injection were analysed statistically. Re-testing of the same subjects after a while shows that, in spite of all efforts to maintain the same experimental conditions, variations do occur in late depression. Time course and duration are particularly affected, while the degree of depression is altered but slightly. The presence of such variations limits this test to evaluation of the influence of other factors only within their already established statistical limits.


Neuromuscular block after intra-arterially injected acetylcholine. 2. Effects of ACTH treatments as possible detectors of desensitization level in the receptor site. It has been suggested that the effect of ACTH in myasthenia gravis may be ascribed to an action involving neuromuscular transmission which favours repolarization processes, with a tendency towards hyperpolarization of the membranes of muscle fibres and motor nerve endings. A similar mechanism has been postulated for the action of ACTH in epilepsy (Klein, 1970). A direct or indirect action on nerve membrane would interfere with depolarization. There is evidence of raised concentration of intracellular potassium and increased outflow of sodium ions which would cause hyperpolarization of the membrane. This paper studies the effect of ACTH on the late block of neuromuscular transmission caused by acetylcholine (ACTH).


Recruitment order of motor units in man: significance of pre-existing state of facilitation. It has been shown in previous investigations that the recruitment order of motor units is different in tonic and in phasic voluntary activity. The significance of the pre-existing state of facilitation in the motoneurone pool for the recruitment of units is studied, using the phasic flexion reflex in the anterior tibial muscle as test reflex. It is shown that the recruitment order of units in a series of reflexes (1) is unstable if the subject does not expect the stimulus; (2) is stable and identical with that in tonic activity if the subject subliminally facilitates the motoneurone pool before the reflex activation; (3) is stable and almost identical with that in tonic activity if the subject expects the stimulus and therefore involuntarily influences the motoneurone pool; (4) is stable and similar to that in phasic voluntary activity if the subject inhibits the motoneurone pool before the activation and the stimulus strength thus consequentially is increased; and (5) is influenced by blockade of the proprioceptive afferent impulses from the muscle. It is concluded that normal man can select in advance the recruitment order of motor units most appropriate for the work intended.


Post-ischaemic paraesthesiae in diabetes mellitus. A quantitative assessment of post-ischaemic paraesthesiae has been made in 50 diabetic subjects and in a group of healthy age-matched controls. The results show a highly significant diminution of the paraesthetic response in the diabetic subjects. The degree of depression of the paraesthetic response was associated with the duration of the disease and the severity of the metabolic abnormality as determined by the degree of insulin dependence. Diabetics with the juvenile onset type of the disease were more adversely affected than those with the maturity onset type. There was no consistent relationship between the degree of depression of the paraesthesiae and the presence of peripheral neuropathy. The significance of these results is discussed in relation to the factors which determine the composition of the ionic micro-environment of myelinated nerve and the level of electrical excitability of the nerve fibre.


Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation. The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 x 10(6). Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 x 10(6) is considerably smaller than the estimates of 6 x 10(6) to 7 x 10(6) determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.


Growth of coliphage T7 in Salmonella typhimurium. A mutant of Salmonella typhimurium was found to be sensitive to killing by coliphage T7 because of an alteration in its surface properties. However, the infections were abortive and studies with (32)P-labeled T7 grown in Escherichia coli B (T7.B) indicated that the phage DNA was restricted by S. typhimurium. When a mutant T7 which survived the restriction and produced plaques on Salmonella (T7.S) was passed through one cycle of growth in E. coli B, its ability to grow in Salmonella was lost, indicating that host-controlled restriction and modification are operative in this system. Restrictionless S. typhimurium mutants were isolated that permit the growth of not only T7.S but also T7.B and coliphage T3. The physiology of T7 production in the restrictionless host is nearly identical to that in Escherichia coli.


Infectivity and RNA patterns as functions of high- and low-dilution passage of murine sarcoma-leukemia virus: evidence for autointerference within an oncornavirus population. Heterogeneity of buoyant density and RNA content of virions of Moloney murine leukemia-sarcoma complex [MSV (MLV)] was the result of passage at low dilution. Heterogeneous stocks revealed two major RNA components in the population, with the smaller component, apparent mol wt 4 x 10(6) to 5 x 10(6), becoming predominant upon serial passage at low dilution. Concomitantly, infectivity titers of both MLV and MSV decreased upon serial passage at low dilution. MSV (MLV) passaged at high dilution retained high titers and a rather homogeneous high-molecular-weight RNA population characteristic of high-buoyant-density virions. Interference of both MLV and MSV replication was demonstrated by employing mixed inocula containing both low- and high-dilution passage stocks of MSV (MLV). In contrast to results with MSV (MLV), MLV freed of MSV by limit dilution did not show heterogeneity of buoyant density or of RNA when propagated at low dilution.


Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties. Four new nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses have been isolated. Although these viruses (designated Ad2(+)ND(2), Ad2(+)ND(3), Ad2(+)ND(4), and Ad2(+)ND(5)) were clonal derivatives of the same Ad2-SV40 hybrid population, they differ significantly from each other and from the previously isolated nondefective hybrid, Ad2(+)ND(1), in their biological properties or in the amount of SV40-specific RNA induced during lytic infection.Like Ad2(+)ND(1), Ad2(+)ND(2), and Ad2(+)ND(4) pass serially in both human embryonic kidney (HEK) and primary African green monkey kidney cells. In contrast, Ad2(+)ND(3) and Ad2(+)ND(5) pass serially only in HEK cells. Ad2(+)ND(2) is like Ad2(+)ND(1) in that it induces the SV40 U antigen, but not SV40 T antigen; however, in contrast to the perinuclear SV40 antigen induced by Ad2(+)ND(1), the SV40 antigen induced by Ad2(+)ND(2) is located peripherally in the cytoplasm as well as in the perinuclear region of infected cells. Ad2(+)ND(4) induces both the SV40 T and U antigens. Ad2(+)ND(3) and Ad2(+)ND(5) do not induce serologically detectable SV40 antigens and are distinguished from each other on the basis of the relative quantities of SV40-specific RNA which they induce. The induction of different SV40-specific functions suggests the incorporation of different segments of SV40 DNA within the genomes of the respective hybrid viruses.


Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VI. Characterization of the DNA from five nondefective hybrid viruses. Certain biophysical characteristics of the DNA from each of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2(+)ND(1), Ad2(+)ND(2), Ad2(+)ND(3), Ad2(+)ND(4), Ad2(+)ND(5)) have been determined. The guanine plus cytosine content varied from 55 to 57% and was not significantly different from that of nonhybrid Ad2 (56%), and the hybrid DNA molecules had mean molecular lengths which were similar to that of the standard, Ad2. The Ad2 and SV40 components of each hybrid were linked by alkali-resistant, presumably covalent bonds. The percentage of SV40 DNA in each hybrid virus was determined by hybridization with SV40 complementary RNA in a calibrated system. The results indicate that each hybrid virus DNA contains a different percentage of SV40 nucleotide sequences. The estimated size of the SV40 DNA component varies from 48,000 daltons for Ad2(+)ND(3) to 840,000 daltons for Ad2(+)ND(4), the latter being equivalent to between one-fourth and one-third of the SV40 genome.


Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses. Five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses have been isolated and found to contain segments of SV40 DNA covalently linked to Ad2 DNA. The quantity of SV40 DNA present is a stable characteristic of each hybrid virus, and varies from less than 5% (in Ad2(+)ND(3)) to more than 30% (in Ad2(+)ND(4)) of the SV40 genome. We have characterized the SV40 portions of these hybrids by relating the SV40-specific RNA sequences transcribed in cells infected with each hybrid virus to those transcribed in cells infected with each of the other hybrid viruses and with SV40 itself. RNA-DNA hybridization-competition experiments indicate that the number of unique SV40 RNA sequences transcribed in infected cells is proportional to the size of the SV40 DNA segment contained within each hybrid and, in the case of the three hybrids which induce detectable SV40-specific antigens, to the number of SV40 antigens induced. Furthermore, the SV40-specific RNA sequences transcribed from any one of the hybrids are completely represented in the RNA transcribed from all other hybrids with longer SV40 segments. Thus, the SV40 DNA regions in the five hybrid viruses appear to contain some nucleotide sequences in common. The SV40-specific RNA transcribed from Ad2(+)ND(4), the hybrid containing the largest SV40 segment, is qualitatively similar to the SV40-specific RNA transcribed early (i.e., prior to viral DNA replication) in SV40 lytic infection. Thus, it appears that no significant amount of late SV40 DNA is transcribed during infection by any of the five nondefective Ad2-SV40 hybrid viruses.


Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1  and Ad2 + ND 3 . The DNA of simian virus 40 (SV40) was transcribed into RNA by Escherichia coli RNA polymerase at 18 to 24 C after synchronization of the initiation of RNA synthesis. After a brief synthetic period the RNA product contained relatively large amounts of sequences derived from a limited segment of SV40 DNA. The source for this pulse-labeled RNA was found to be a portion of the segment of SV40 DNA included within the nondefective adenovirus (Ad)-SV40 hybrid viruses, Ad2(+)ND(1) and Ad2(+)ND(3). After synthesis with [gamma-(32)P] ATP, Ad2(+)ND(1) and Ad2(+)ND(3) DNA transcripts contained an initial sequence missing from Ad2 transcripts. This sequence was identified as an initiation sequence for polymerase transcription of the SV40 DNA. Thus, there is a preferred site for initiation of in vitro transcription on the segment of SV40 DNA common to the nondefective Ad2(+)ND(1) and Ad2(+)ND(3) hybrid viruses.


Temperature-sensitive simian virus 40-transformed cells: phenomena accompanying transition from the transformed to the "normal" state. Temperature-sensitive simian virus (SV 40)-transformed 3T3 cells (tsSV3T3), which express the transformed phenotype when growing at 32 C but not at 39 C, were used to study changes in growth behavior during shift-up or shift-down experiments. In cultures of tsSV3T3 cells which had reached or were beyond monolayer density at 32 C, DNA synthesis reached very low levels within 24 to 48 h after shift-up. When cells which had been allowed to grow to high densities at 32 C were shifted to 39 C, not only cell growth stopped, but within two to three days the cultures shed a large number of cells into the medium. These cells were nonviable, and shedding stopped only when the number of cells attached had been reduced to that characteristic of the saturation density at 39 C. The remaining attached cells were viable and after the shift to 32 C were again able to grow from the monolayer to high cell densities. This behavior has been compared with that of normal 3T3 and wild-type SV3T3 cells under different conditions. We have also isolated new tsSV3T3 lines, using cells which had been infected with non-mutagenized wild-type SV40. This further demonstrates that the temperature sensitivity of these lines is due to a cellular rather than a viral mutation.


Structural rearrangement and subunit composition of RNA from released Soehner-Dmochowski murine sarcoma virions. Two types of genomic, high-molecular-weight RNA species were found in Soehner-Dmochowski murine sarcoma virions released from virus-induced rat tumor cells grown in tissue culture. The type of RNA species observed depended on the length of exposure of the tumor cells to radioactive precursor. Early RNA of virions labeled up to 4 h with radioactive uridine had a sedimentation coefficient of 50S, and late RNA of virions labeled for 24 h had a sedimentation coefficient of 58S. Thermal transitions of early and late RNA indicated a difference in the configuration or structure of these two types of RNA. The late RNA may represent either a different configurational state of the early RNA or an aggregate molecule of two early RNA components joined together. Heat dissociation revealed that the major subunit of both RNA types was a 28S species, which was not susceptible to degradation by the addition of micrococcal nuclease to virions. A transitional, intermediate RNA species with a sedimentation coefficient of 37 to 40S was detected when early RNA was dissociated by dimethyl sulfoxide or heat at temperatures suboptimal for complete conversion. No free RNA subunit components were detected in virions harvested at intervals as short as 30 s or 5 min. A model for the assembly of genomic RNA from 28S RNA subunits is proposed.


Inhibition in the joining of DNA intermediates to growing simian virus 40 chains. Viral DNA synthesis was inhibited for 1 h by the addition of 5-fluorodeoxyuridine (FUdR) to simian virus 40 (SV40)-infected cultures at 28 to 30 h postinfection. The subsequent addition of (3)H-thymidine to the inhibited cultures reverses the effect of the inhibitor, and during a 1-min labeling period there is rapid synthesis of SV40 DNA. By alkaline sedimentation analysis, it is observed that in FUdR-treated cultures there is synthesis of 4S SV40 DNA intermediates but there is a block in the joining of these intermediates to growing SV40 chain cultures. In addition to 4S fragments that are associated with replicating SV40 molecules, there is accumulation of SV40 DNA in the 6 to 8S region which is observed in neutral sucrose gradients. In an inhibited culture that is pulsed for 1 min with (3)H-thymidine and then chased for 10 min, accumulation of a Component II (Comp. II)-like material is observed. This Comp. II has the same neutral sedimentation characteristics and yields the same R(I) restriction endonuclease product as does authentic Comp. II. However, in alkali it is seen that it is composed of fragmented SV40 DNA. The basis for the failure of 4S fragments to join to growing SV40 chains is discussed. A model in which there is a requirement for two DNA polymerases and a ligase to permit SV40 DNA chain growth is proposed which is consistent with the data presented.


Avian oncornavirus group-specific antigen: detection and quantification by radioimmunoassay. A double-antibody competitive-inhibition radioimmunoassay for avian group-specific antigen is described. A viral protein preparation, consisting primarily of gs-1, was labeled with radioiodine. Hamster and rabbit antisera were reacted with the labeled antigen, and the resultant antibody-antigen complexes were precipitated with the appropriate antiglobulin. Standard curves based on the inhibition of binding of labeled antigen to antibody after preincubation with unlabeled antigen were prepared. These showed the lower limit of sensitivity to be 2 to 10 ng of unlabeled protein when labeled antigen of 2,000 to 5,000 counts per min per ng of specific activity was used in the assay. Extracts of avian oncornavirus-infected cells, likewise, were able to inhibit binding of labeled antigen. This technique will be very useful for the quantification of small amounts of oncornavirus group-specific protein.


Relationship of reticuloendotheliosis virus to the avian tumor viruses: nucleic acid and polypeptide composition. The RNA content and polypeptide composition of reticuloendotheliosis virus (REV) was compared to that of C-type RNA tumor viruses. Two RNA species with approximate sedimentation values of 64S and 4S were observed after sucrose gradient centrifugation of RNA extracted from purified REV. The high-molecular-weight RNA species of REV sedimented slightly faster than that of the Bryan strain of Rous sarcoma virus (RSV). Although these characteristics were consistent with those of other C-type RNA tumor viruses, significant differences were observed when the polypeptide composition of REV was compared with that of RSV possessing envelope determinants of Rous-associated virus RAV-2 and RAV-3. Five polypeptides of which two were glycosylated were resolved by polyacrylamide gel electrophoresis. The major nonglycosylated polypeptide of REV did not comigrate with that of RSV (RAV-2)-RSV(RAV-3). The majority of the group-specific antigen reactivity resides in this major nonglycosylated polypeptide of avian tumor viruses and comigrates when proteins of several avian tumor viruses are subjected to coelectrophoresis. This difference in the migration of the major polypeptide of REV and RSV(RAV-2)-RSV(RAV-3) may explain the absence of avian tumor virus group-specific antigen in REV.


Quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with Rous sarcoma virus. A sensitive and quantitative nucleic acid hybridization assay for the detection of radioactively labeled avian tumor virus-specific RNA in infected chicken cells has been developed. In our experiments we made use of the fact that DNA synthesized by virions of avian myeloblastosis virus in the presence of actinomycin D (AMV DNA) is complementary to at least 35% of the sequences of 70S RNA from the Schmidt-Ruppin strain (SRV) of Rous sarcoma virus. Annealing of radioactive RNA (either SRV RNA or RNA extensively purified from SRV-infected chicken cells) with AMV DNA followed by ribonuclease digestion and Sephadex chromatography yielded products which were characterized as avian tumor virus-specific RNA-DNA hybrids by hybridization competition with unlabeled 70S AMV RNA, equilibrium density-gradient centrifugation in Cs(2)SO(4) gradients, and by analysis of their ribonucleotide composition. The amount of viral RNA synthesized during pulse labeling with (3)H-uridine could be quantitated by the addition of an internal standard consisting of (32)P-labeled SRV RNA prior to purification and hybridization. This quantitative assay was used to determine that, in SRV-infected chicken cells labeled for increasing lengths of time with (3)H-uridine, labeled viral RNA appeared first in a nuclear fraction, then in a cytoplasmic fraction, and still later in mature virions. This observation is consistent with the hypothesis that RNA tumor virus RNA is synthesized in the nucleus of infected cells.


Stimulation of the collagen biosynthetic pathway by factors isolated from experimentally-injured liver.


Action of intracerebroventricular dibutyryl cyclic AMP on amobarbital anaesthesia in rats.


Amniotic fluid phospholipids in early gestation.


Tectal activity and eye movements during paradoxical sleep in cats.


Pulmonary surfactant. Is the fetal adrenal necessary?


The effects of conditioned behaviour and environmental factors on plasma corticosteroid levels in pigs.


Electrographic correlates of sleep behavior in a primitive mammal, the armadillo Dasypus novemcinctus.


Rewarding and aversive brain stimulation opposite effects on medial thalamic units.


The role of the dorsal nerves of the penis in the sexual behaviour of the male rhesus monkey.


Path neuron system of medial forebrain bundle as a possible substrate for hypothalamic self-stimulation.


Drinking to intracerebral angiotensin II and carbachol: dose-response relationships and ionic involvement.


Cellular responses to cyclic AMP.

Cellular responses to cyclic AMP.


Enzymatic properties of a repressible acid phosphatase in Neurospora crassa.


Neoplasms of the sella turcica region in Ibadan, Nigeria.


Homogenous immunochemical drug assays.

Homogenous immunochemical drug assays.


Immunological assays for drugs in biological samples.


Comparative evaluation of methods for laboratory diagnosis of herpetic eye disease.


Quillaiate of choline iodide: a new monoquaternary neuromuscular blocking agent.


Subcellular distribution of adenyl cyclase and phosphodiesterase in Acanthamoeba palestinensis.


Studies with phosphodiesterase. 3. Two forms of the enzyme from human blood platelets.


Incisional and peritoneal infection after emergency celiotomy.


Substrate specificity of hog plasma angiotensin-converting enzyme.


Histamine sensitizing activity of lentinan, an antitumour polysaccharide.


The renin-angiotensin system in urinephrectomized adrenalectomized rats.


A quantitative investigation of the response to injury of the central nervous system of rats treated with ACTH and triiodothyronine.


Radioimmunoassay of angiotensin II in rat plasma.


The primary tissue culture of rat adult decapsulated adrenal glands: radioautographic studies on the metabolic effects of   1-24 -ACTH.


Strength modification of polycarboxylate cement with fillers.


Further studies on the chromatographic behaviour of dinucleoside monophosphates.


Preparative argentation column chromatography for separation of sterols from orange vesicles.


Application of direct spectrophotometry to the analysis of chromatograms. 3. A new micro thin-layer chromatoplate and its application to the analysis of tocopherols.


Detection of naturally fluorescent pesticides on silica gel layers.


The formation of synapses in reinnervated and cross-reinnervated adult avian muscle. 1. A study has been made of the formation of synapses in spontaneously reinnervated and cross-reinnervated anterior latissimus dorsi (ALD) and posterior latissimus dorsi (PLD) muscles of adult fowls.2. Denervated ALD and PLD muscle fibres have a uniform and high sensitivity to iontophoretically applied acetylcholine (ACh). During early reinnervation the sensitivity distribution to ACh of the ALD muscle fibres begins to return to normal before synaptic potentials can be evoked. The normal ACh sensitivity distribution of PLD muscle fibres is also restored after reinnervation. After cross-reinnervation of the ALD and PLD muscles the ACh sensitivity distribution of many of the muscle fibres is again restored to normal.3. Reinnervating and cross-reinnervating ALD nerve terminals showed a greater than normal degree of facilitation of transmitter release when a test impulse was applied at various intervals after a conditioning impulse. Cross-reinnervating PLD nerve terminals showed facilitation of transmitter release rather than the normal depression in a conditioning-test impulse sequence.4. The distribution of nerve terminals over the surface of spontaneously reinnervated and cross-reinnervated ALD and PLD muscle fibres has been determined from an examination of the sensitivity distribution to applied ACh, the graded versus all-or-none nature of the evoked potential and the distribution of cholinesterase stained synapses.5. The results suggest that the innervation pattern of individual ALD and PLD muscle fibres is restored both after spontaneous reinnervation and cross-reinnervation.


Effects of muscle stretch on transmitter release at end-plates of rat diaphragm and frog sartorius muscle. 1. Miniature end-plate potentials (m.e.p.p.s) and end-plate potentials were recorded intracellularly at neuromuscular junctions of the frog sartorius muscle and the rat diaphragm before, during and after muscle stretch.2. Stretch increased the frequency of the m.e.p.p.s and the mean quantum content (m) at end-plates of the sartorius muscle.3. Stretch enhanced m.e.p.p. frequency of the sartorius muscle in normal Ringer solution as well as in solutions high in magnesium and low in calcium content. In addition, an increase in frequency occurred in the presence of tetrodotoxin and in the absence of neostigmine methylsulphate.4. The magnitude of the increase in m.e.p.p. frequency in the sartorius muscle was negatively correlated with the control frequency.5. In contrast to the above findings at sartorius end-plates, muscle stretch did not affect the frequency of m.e.p.p.s or the m values at end-plates of the rat diaphragm.


The orderly recruitment of human motor units during voluntary isometric contractions. 1. The contractile properties of human motor units from the first dorsal interosseus muscle of the hand were studied during voluntary isometric contractions using recently developed techniques.2. The twitch tensions produced by motor units varied widely from about 0.1-10 g. The twitch tension of a motor unit varied nearly linearly as a function of the level of voluntary force at which it was recruited over the entire range of forces studied (0-2 kg).3. The number of additional motor units recruited during a given increment in force declined sharply at high levels of voluntary force. This suggests that even though the high threshold units generate more tension, the contribution of recruitment to increases in voluntary force declines at higher force levels.4. Contraction times for these motor units varied from 30 to 100 msec. Over 80% had contraction times less than 70 msec, and might be classed as fast twitch motor units. The larger motor units, which were recruited at higher threshold forces, tended to have shorter contraction times than the smaller units.


Maternal infections and the developing foetus.


Patterning mechanisms, patterned reflexes, and attack behavior in cats.


Maternal cytomegalovirus excretion and perinatal infection.


Influence of levodopa on serum levels of anterior pituitary hormones in man.


Neural pathways mediating adrenocortical responses to photic and acoustic stimuli.


Management of nursing care on a psychiatric service.


Patterns of development of adenyl cyclase activity and norepinephrine responsiveness in the rat.


Sleep patterns in hyperphenylalaninemia: a lesson on serotonin to be learned from phenylketonuria.


Inhibition of glucose oxidation and fatty acid synthesis in liver slices from fed, fasted and fasted-refed rats by glucagon, epinephrine and cyclic adenosine-3',5'-monophosphate.


Characterization of guinea pig C-type virus.


Effect of the synthetic peptide, Sq 20,881, on distribution of blood flow in the rat.


HL-A antigens of human tumor-derived cell lines and viral transformed fibroblasts in culture.


Estrogen-topographical localization of estrogen-concentrating cells in the rat spinal cord following  3 H-estradiol administration.


Adrenal and plasma corticosterone and pituitary and plasma ACTH in alloxan diabetic rats.


Immunosuppression and increased susceptibility to Japaneses B encephalitis virus in Trichinella spiralis-infected mice.


Effects of prostaglandins PGE 2  and PGF 2   on the adenyl cyclase activity of various segments of the immature rabbit oviduct and uterus.


Endogenous triglyceride and glycogen in perfused rat hearts.


B-scan ultrasound in the evaluation of pediatric abdominal masses.


Mammary duct injection.

Mammary duct injection.


The incidence of osseous involvement in lung cancer, with special reference to the development of osteoblastic changes.


Evidence on the constitutive nature of a p-nitrophenylphosphatase of Streptococcus mutans.


Significance of a p-nitrophenylphosphatase for Streptococcus mutans.


Epidemiologic characteristics of presumed ocular histoplasmosis.


Changes in lipid values and lipoprotein patterns of serum samples contaminated with bacteria.


Automated stopped-flow kinetic analysis of serum enzymes.


pH dependence of the renin reaction: a modification of commercial renin activity radioimmunoassay kits.


Corneal degeneration.

Corneal degeneration.


Antiviral activity of isoprinosine in vitro and in vivo.


Microsomal electron transport: tetrazolium reduction by rat liver microsomal NADPH-cytochrome c reductase.


Effect of the pH of the incubation medium on glycolysis and respiration in Saccharomyces cerevisiae.


Bovine hepatic fructose 1,6-diphosphatase: modification of the catalytic and allosteric properties by sulfhydryl reagents.


Protein synthesis directed by encephalomyocarditis virus mRNA. 3. Discrete polypeptides translated from a monocistronic messenger in vitro.


Interaction of viral RNA with Escherichia coli. 3. Synthesis of poliovirus-specific RNA directed by isolated poliovirus RNA.


Purification and properties of cyclic phosphodiesterase: 3'-nucleotidase, a periplasmic enzyme of Haemophilus influenzae.


On the source of GABA-containing terminals in the substantia nigra. Electron microscopic autoradiographic and biochemical studies.


Neuronal activity in the association cortex of the cat during sleep, wakefulness and anesthesia.


Compensatory changes in REM sleep time of cats during ad libitum sleep and following brief REM sleep deprivation.


Miniature end-plate potentials: evidence that the intervals are not fit by a Poisson distribution.


Action of a GABA-derivative on postsynaptic potentials and membrane properties of cats' spinal motoneurones.


Some effects of vinblastine and colchicine on neuromuscular transmission.


Scorpion venom-induced discharge of catecholamines accompanied by hypertension.


Ribosome-covered vesicles associated with synthesis and secretion of invertase in yeast.


The long-acting thyroid stimulator.

The long-acting thyroid stimulator.


Effects of ovarian hormones at the subcellular level.


Endocrinological implications of prostaglandins.


Steroid hormones and the differentiation of the central nervous system.


Polygraphic profile of oral tardive dyskinesia and of rabbit syndrome: for quantitative and qualitative evaluation.


Gout and pseudogout.

Gout and pseudogout.


Ultrastructural and lipid biochemical comparisons of human eruptive, tuberous and planar xanthomas.


Virus-induced changes in the endogenous pathway of thymidine in animal cells.


An ultrastructural analysis of the photoreceptors of the squid and their synaptic connections. II. Intraretinal synapses and plexus.


Indirect haemagglutination reaction with varicella-zoster virus antigen.


Identification of a major basic protein in guinea pig eosinophil granules. Elucidation of the functions of the eosinophil might be accomplished by analysis of the granule constituents. We have purified eosinophils (93% or greater) from the peritoneal cavity of the guinea pig and have investigated a variety of methods to disrupt cells and liberate intact granules. Lysis in 0.34 M sucrose gave the best yield of granules and these had the characteristic morphology of eosinophil granules when examined by electron microscopy. Granules were solubilized by a variety of treatments and the solutions analyzed by polyacrylamide electrophoresis at pH 3 in 6 M urea. Comparison of the electrophoretic patterns of solubilized eosinophil and neutrophil granules revealed a difference: a major portion (53+/-3%; x +/-1 SE) of the protein from the eosinophil granule migrated as a single component. This major band protein has a molecular weight between 6,000 and 12,000 daltons and a pI of 10 or greater. Analysis of eosinophil granule constituents on Sephadex G-50 revealed two main peaks; peak 1 possessed peroxidase activity and peak 2 contained the major band protein. These studies indicate that eosinophil granules contain a cationic protein of low molecular weight which lacks peroxidase activity and which accounts for greater than 50% of granule protein.


An analysis of cylindroma of the head and neck.


Plasma renin activity during levodopa therapy. Significance of long- and short-term treatment.


Stand up and be diagnosed.

Stand up and be diagnosed.


Reversible inactivation of liver microsomal pyrophosphatase.


Effect of clofibrate on arginine-induced insulin and glucagon secretion.


Effects of a purified growth hormone releasing factor on growth hormone secretion and pituitary cyclic nucleotide content.


Precursors of plasma triglyceride fatty acids in obesity.


Triglyceride removal efficiency and lipoprotein lipases: effects of Oxandrolone.


Increase of microsomal glucose-6-phosphatase activity after chronic ethanol administration.


Sensory transduction. II. Visual receptors. Quantum efficiency of isomerization.


Brain, speech and consciousness.

Brain, speech and consciousness.


Immunization of chickens for the control of infectious bursal disease.


The inheritance of resistance to subgroup C leukosis-sarcoma viruses in New Hampshire chickens.


Horizontal transmission of turkey herpesvirus to chickens. 2. Some factors affecting transmission.


Modification of high temperature and ACTH induced immunodepression by metyrapone.


Conversion of glutamic acid to proline in the chick.


Regression of Rous sarcomas in two lines of chickens.


Characteristics of physicians interested in psychiatric education.


Ovarian tumors in pregnancy. A private hospital ten year survey.


Carbohydrate metabolism during differentiation (sclerotization) of the myxomycete Physarum flavicomum.


Cortico-fugal facilitation of thalamic transmission.


Discussion: functional studies of thalamic internuclear interactions.


The second international standard for polymyxin B. Since supplies of the first International Standard for Polymyxin B were exhausted, it was replaced by a second international standard the potency of which was estimated from the results of a collaborative assay carried out by 5 laboratories in 4 countries. The wide variations in the results probably resulted from difficulties experienced in handling the first international standard. The potency finally agreed upon by the collaborating laboratories, on the basis of the overall mean values obtained after rejection of the most discrepant assays, was 8 403 IU/mg. That value was accepted by the WHO Expert Committee on Biological Standardization (1970), which consequently defined the International Unit of polymyxin B as the activity contained in 0.000119 mg of the second international standard.


Intraaortic balloon pumping for ventricular septal defect or mitral regurgitation complicating acute myocardial infarction.


Effects of infections on pregnancy risk.


Antibody tests in streptococcal pharyngitis. Streptozyme versus conventional methods.


Labial fusion: a cause of recurrent urinary tract infections.


Differentiation of human cortical neurons: proteins of subcellular particles.


Enhancement of senescence in low passage human embryonic lung cells by an agent extracted from phase 3 cells.


Stepwise accumulation of autoregulated enzyme activities during the cell cycle of the eucaryote Chlorella.


The effect of photosensitizing dyes on the  3 H-tymidine incorporation of cells growth in vitro.


Myogenic cells derived from murine sarcoma virus induced mouse tumours: DNA synthesis and the effects of Simian virus 40 infection.


Activation of nuclear chromatin and the release from contact-inhibition of 3T3 cells.


Maturation-stimulating effect of brain extract and dibutyryl cyclic AMP on dissociated embryonic brain cells in culture.


Differential sensitivity of antigen- and mitogen-stimulated human leucocytes to prolonged inhibition of potassium transport.


Citric acid-sonication method for the isolation of nucleoli.


Deoxyribonuclease in sea urchin embryos. Comparison of the activity present in developing embryos, in nuclei, and in mitochondria.


The kinetics of transmitter release in myasthenia gravis. I. An electrophysiological analysis of the storage of transmitter.


Phosphatases from rice bran.

Phosphatases from rice bran.


Inhibition of hexosephosphate aminotransferase activity in vitro by tolbutamide.


Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. 3. Lactate dehydrogenase.


Studies on L-glycerol-3-phosphate dehydrogenases in Japanese Quail, Coturnix coturnix japonica. II. Comparison of activity of mitochondrial and cytoplasmic L-glycerol-3-phosphate dehydrogenases in testes and liver of developing male Japanese quail.


DNA-dependent RNA polymerase from Ehrlich ascites tumor cells. I. Partial purification and characterization of RNA polymerase II.


Biochemical studies on sulfate-reducing bacteria. XII. Some properties of flavodoxin from Desulfovibrio vulgaris.


The crystal structure of bonito (Katsuo) ferrocytochrome c at 2.3 A resolution.


Synthetic analogues of polynucleotides. IX. Synthesis of 3'-O-carboxymethyl-2'-deoxyribonucleosides and their use in the synthesis of an analogue of 2'-deoxyadenylyl-(3' leads to 5'-)-thymidine 3'-phosphate.


The influence of silica gel G on the quantitative analysis of lipid phosphorus.


Fine-structural aspects of macrogametogenesis in Eimeria magna.


The effects of actinomycin D, gibberellin A7, and cyclic AMP on mating of Chlamydomonas reinhardtii.


Growth phase and the number of phosphorylation sites in the mitochondrial electron transport chain of Acanthamoeba castellanii.


Current therapy of Wilms' tumor.

Current therapy of Wilms' tumor.


Juvenile nasopharyngeal angiofibroma.

Juvenile nasopharyngeal angiofibroma.


Otoneurology.

Otoneurology.


Peripheral nerve surgery.

Peripheral nerve surgery.


General neurophysiology: bioelectric aspects.


Effects of liothyronine-induced hypermetabolism on dipyrone metabolism in man.


Regional physiology of the nervous system.


Neuropathology.

Neuropathology.


Clinical neurology.

Clinical neurology.


Effect of REM sleep deprivation on electric shock threshold in rats.


Some aspects of nucleoside and nucleotide metabolism in the peripheral leukocytes during kidney allograft rejection.


Correlations between some economic, social and infectious factors and the pathology of pregnancy and of the neonate.


Comparative studies on some herpes simplex virus strains with different locations. II. Study of the virulence character.


The action of human immunoglobulins in experimental herpes infection in the newborn rat.


Atherosclerosis and the arterial smooth muscle cell: Proliferation of smooth muscle is a key event in the genesis of the lesions of atherosclerosis.


Slow viruses: role in persistent disease.


Postlesion axonal growth produces permanent functional connections. Lesions of the entorhinal cortex in neonatal rats cause the commissural projections to the dentate gyrus to spread from their normal location in the inner molecular layer into the outer molecular layer, a region normally occupied by afferents from the entorhinal cortex. These lesions also cause the short-latency response to commissural stimulation to spread into the outer molecular layer, a result suggesting that these abnormally located connections are operative.


Angiotensin-sodium interaction in blood pressure maintenance of renal hypertensive and normotensive rats. A specific inhibitor of angiotensin II was used in rats to investigate whether angiotensin is involved in the maintenance of blood pressure in one-kidney Goldblatt hypertension, in which plasma renin levels are not usually increased. The inhibitor produced marked falls in blood pressure, often down to normal levels in the hypertensive animals only when they were depleted in sodium and not after sodium repletion. Much lesser but still significant falls in blood pressure were also produced in normotensive sodium-depleted rats but not in repleted rats. We conclude that the importance of angiotensin for maintaining blood pressure is largely determined by its relation to available sodium or fluid volume, since the renin component in maintenance of either the hypertensive or the normotensive state could be exposed only by sodium deprivation. Therefore, volume expansion per se or other pressor factors may be involved in maintaining blood pressure of these sodium-replete normotensive or hypertensive animals.


Delta 9 -tetrahydrocannabinol and ethanol: differential effects on sympathetic activity in differing environmental setting. Serum dopamine beta-hydroxylase activity, a useful biochemical index of peripheral sympathetic nervous activity, was measured in rats treated with Delta(9)-tetrahydrocannabinol or ethanol or both substances. After 7 days of treatment with either substance, serum dopamine beta-hydroxylase activity decreased significantly. Combined treatment with both agents enhanced the effects of each given alone. In rats subjected to immobilization stress, treatment with Delta(9)- tetrahydrocannabinol appeared to potentiate the stress-induced increase in serum enzyme activity. Treatment with ethanol, with or without Delta(9)-tetrahydrocannabinol, effectively blocked this increase in enzyme activity. These results show that both substances have significant effects on the sympathetic nervous system which are critically influenced by environmental setting.


The effect of iodine prophylaxis on the incidence of endemic cretinism.


Effect of iodine correction early in fetal life on intelligence quotient. A preliminary report.


Prophylaxis of endemic goiter with iodized oil in rural Peru.


The optimal program for prophylaxis of endemic goiter with iodized oil.


IQ tests and intelligence in endemic cretinism.


Tetrahydroisoquinoline alkaloids: uptake, storage, and secretion by the adrenal medulla and by adrenergic nerves.


Experimental study of sleep in chronic alcoholics before, during, and after four days of heavy drinking with a nondrinking comparison.


The effects of alcohol on dreams and hallucinations in alcohol addicts.


Certain validity problems in computer analysis of clinical EEGS.


Electroencephalographic and other evidence for mixed depressant and stimulant actions of alcoholic beverages.


Recent studies in man and animal of peripheral nerve and muscle dysfunction associated with chronic alcoholism.


Seryl transfer ribonucleic acid synthetase of Escherichia coli B, Enzyme-substrate interactions.


Seryl transfer ribonucleic acid synthetase of Escherichia coli B. Purification, subunit structure, and behavior in the acylation reaction.


Two physically and functionally distinct forms of eukaryotic 40S ribosomal subunits.


Characterization and poly(adenylic acid) content of Ehrlich ascites cell ribonucleic acids fractionated on unmodified cellulose columns.


Phospholipid biosynthesis in the membranes of immature and mature red blood cells.


A filter assay for steroid hormone receptors.


Oxidation of 3,3'-diaminobenzidine by rat liver mitochondria.


Lipid model membranes. Characterization of mixed phospholipid vesicles.


Thorotrast-induced hepatoma presenting as hyperparathyroidism.


Radiation-induced extraskeletal osteosarcoma.


Ultrastructure of bronchial oncocytoma.

Ultrastructure of bronchial oncocytoma.


Structure and size prognosis in nephroblastoma.


Oat cell carcinoma of the pancreas with ectopic ACTH secretion.


Cytoplasmic and mitochondrial NAD + -NADH 2  ratios in rat brain and liver: effect of hypoxia.


An electrophoretic study of some hydrolases from an OP-susceptible and an OP-resistant strain of the green peach aphid, Myzus persicae (Sulz.).


Enhancement of potassium influx, in baby hamster kidney cells and chicken erythrocytes, during adsorption of parainfluenza 1 (Sendai) virus.


Phosphatidylinositol metabolism in cells receiving extracellular stimulation.


Formation of 2,3-pentanediol from 2,3-pentanedione and acetylethylcarbinol by diacetyl(acetoin)reductase from Aerobacter aerogenes. A possible new pathway.


DNA synthesis in isolated nuclei and isolated mitochondria of herpes simplex virus infected HeLa cells.


Structural properties of the ligand binding sites of murine myeloma proteins.


Specific killing of RSV-transformed cells in vitro by lymphoid cells from RSV tumor-bearing chickens.


The variable influence of host microflora and intercurrent infections on immunological competence and carcinogenesis.


Analysis of cell membrane antigens using radioiodinated, purified antibodies. II. A factor in the serum of hamsters bearing SV 40 -induced tumors which reacts with antibodies to SV 40 -transformed cell membranes.


Analysis of cell membrane antigens using radioiodinated, purified antibodies. I. Solubilization and preliminary fractionation of membrane antigens of cells transformed by SV 40  virus.


Nutritional effectiveness of 1,25-dihydroxycholecalciferol in preventing rickets in chicks.


Experimental Listeria cystitis. II. Further evidence of the epithelial phase in experimental Listeria infection. An electron microscopic study.


Ultrastructure of the proximal convoluted tubules during repair following hormonally induced necrosis in rat kidney.


Histochemical and ultrastructural investigation on the endocrine cells of the stomach in hypersecreting (pylorus-ligated) rats.


Primary carcinoma of the extrahepatic bile ducts.


Metastases and primary neoplasms of the stomach in patients with breast cancer.


Measurement of plasma renin activity by angiotensin I radioimmunoassay. II. Clinical results.


Rabbit red cell cyclic AMP-dependent protein kinase. I. Reversible subunit interaction.


Respiratory control and oxidative phosphorylation of the cytochrome c-cytochrome oxidase complex.


A rapid method for the isolation of large quantities of rat liver parenchymal cells with high anabolic rates.


Purification of anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase from Salmonella typhimurium using affinity chromatography: resolution of monomeric and dimeric forms.


Cortisol receptors in rabbit fetal lung.


Comparison of the Ca ++  requirement for the steroidogenic effect of ACTH acid dibutyryl cyclic AMP in rat adrenal cell suspension.


Contribution of "substrate shuttles" in hepatic alcohol metabolism in chronic alcoholism.


Demonstration of a glycoprotein which is associated with a purified myelin fraction from rat brain.


Mitochondrial DNA and glucose repression in yeast.


Chain reversals in proteins.

Chain reversals in proteins.


Mössbauer effect in bacterial catalase.

Mössbauer effect in bacterial catalase.


A hydrogen-deuterium exchange study of membranous cytochrome oxidase.


Turbidimetric estimation of chylomicrons and very low density lipoproteins in human sera after precipitation by sodium lauryl sulfate.


Electron microscopic evidence of the subviral oncogenic particles (virosomes) isolated from the mitochondria of Rous sarcoma cells.


Status of credentialing for health personnel.


The influence of in vivo administered chloramphenicol and oxytetracycline on some mitochondrial enzymes of rat-small-intestinal epithelium: histochemical data.


Regulation of cyclic AMP levels and aggregation in human platelets by prostaglandin E   .


Monolayer cell culture of neonatal rat pancreas: an ultrastructural and biochemical study of functioning endocrine cells.


Autopsy findings in a long-surviving liver recipient.


The structures of the principal glycerolipids of pig liver.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 28-1973.


Epidemiology of slipped capital femoral epiphysis: a review of the literature.


Revolutions and cyclical rhythms in prenatal life: fetal respiratory movements rediscovered.


Interstitial pneumonia and cytomegalovirus infection as complications of human marrow transplantation.


A simple method for evaluating the immunosuppressive potency of antimouse lymphocytic serum.


Histological and ultrastructural studies of the basal disk of Hydra. II. Nerve cells and other epithelial cells.


Cochlear pathology in viral disease.

Cochlear pathology in viral disease.


Spiral ganglion virus-like particles of guinea pigs. Further observations.


Clinical studies in sudden deafness.

Clinical studies in sudden deafness.


The role of viruses in sudden deafness.

The role of viruses in sudden deafness.


Clinical measures of 8th nerve function.


Behavioral studies of noise-induced hearing loss in primates: loudness recruitment.


Public policy toward environment 1973: a review and appraisal. The seabed.


Thermal enhancement of DNA damage by an alkylating agent in human cells.


The role of superoxide and singlet oxygen in lipid peroxidation promoted by xanthine oxidase.


Resonance Raman spectroscopy of cytochrome c oxidase and electron transport particles with excitation near the Soret band.


Translation of AKR-murine leukemia viral RNA in an E. coli cell-free system.


Pituitary hormone releasing or inhibiting activity of metal ions present in hypothalamic extracts.


Action of methanol on the assocation of ribosomal subunits and its effect on the GTPase activity of elongation factor G.


CRF-stimulated biosynthesis of ACTH in a cell-free system from rat anterior pituitaries.


Effect of pyrimidine and ribose modifications on the antiviral activity of synthetic polynucleotides.


Simian virus 40 rapidly lowers cAMP levels in mouse cells.


The principal glucocorticoid binding macromolecule in hepatoma cells in culture is similar to corticosteroid binder II of rat liver cytosol.


Detection of three kinetic phases in the thermal unfolding of ferricytochrome c.


Inhibition of ACTH-stimulated steroidogenesis in isolated rat adrenal cells treated with neuraminidase.


Thermodynamic studies of the opening of the heme crevice of ferricytochrome c.


Effects of insulin, epinephrine, and cyclic adenosine monophosphate on pyruvate dehydrogenase of adipose tissue.


On the complexation of ferric ions by reduced nicotinamide adenine dinucleotide.


Lateral phase separation in phospholipid membranes.


Proton spin lattice relaxation time measurements at 90MHz and 270 MHz.


Amino acid sequence of a cyanogen bromide cleavage peptide from hens egg phosvitin.


Lactate dehydrogenase: electrophoretic behaviour, electron microscopy and structure.


Isolectins from wax bean with differential agglutination of normal and transformed mammalian cells.


Anion-binding and the state of copper in caeruloplasmin.


Virological investigations in chronic prostatitis.


Significance of upward gaze palsy (Parinaud's syndrome) in hydrocephalus due to shunt malfunction.


Clostridia. Ubiquitous and still lethal.


Synthesis of cholesterol in hypercholesterolemia and its relationship to plasma trigylcerides.


Studies on the de novo biosynthesis of NAD in Escherichia coli. V. Properties of the quinolinic acid synthetase system.


Stimulation of sensitivity of the glycogen phosphorylase-converting system in skeletal muscle by metabolic inhibitors.


Investigation of procedures for subcellular fractionation of rabbit aortic tissue.


Concanavalin A as a probe of phosphomannan molecular structure.


Quantum chemical study of the mechanism of collagen proline hydroxylation.


Factors affecting the synthesis and release of the extracellular protease of Aspergillus oryzae NRRL 2160.


Basal and hormone-stimulated adenylate cyclase in liver plasma membranes: measurement by radioimmunoassay of cyclic AMP.


Polysaccharides of SV 40-transformed green monkey kidney cells.


Cerebral amino acids of rats deprived of paradoxical sleep.


Cyclic nucleotide phosphodiesterase activity in the plasma and erythrocytes of normal and diabetic rats.


The effect of placebo and drugs on human sleep.


The distribution of free myo-inositol in fluids, spermatozoa, and tissues of the bull genital tract and observations on its uptake by the rabbit epididymis.


Calcium transport through carbon tetrachloride solutions of vitamin D 3 .


The early ambulation of patients after myocardial infarction.


Mastocalcergy in the mouse.

Mastocalcergy in the mouse.


A study of the direct o-toluidine blood glucose determination.


A stable plasma lipoprotein and lecithin cholesterol acyltransferase control material.


Inhibitors of the growth and aggregation of calcium oxalate crystals in vitro.


Increased bone formation in rabbits following intravenous and intra-osseous injection of zinc beryllium silicate.


Difficulties of the differential diagnosis in a medulloblastoma. A casuistic contribution.


Glycogen metabolism in human hormone-producing trophoblastic cells in continuous culture--II. Characterization of BeWo glycogen phosphorylase.


Metabolic oscillations and food intake.

Metabolic oscillations and food intake.


The adnormal biochemistry of inherited disorders of lipid metabolism.


Current enigmas in cancer research.

Current enigmas in cancer research.


Genetic control of melanin metabolism within the melanin unit of mammalian epidermis.


Dermatoglyphics.

Dermatoglyphics.


Ophthalmologic, biochemical, platelet, and ultrastructural defects in the various types of oculocutaneous albinism.


The inheritance of psoriasis.

The inheritance of psoriasis.


Primary glioblastoma multiforme of the spinal cord in infancy and childhood.


Divalent ion metabolism and the kidney.

Divalent ion metabolism and the kidney.


A possible relationship between persistent hepatitis B antigenaemia and renal disease in Southern African Bantu.


Combination chemotherapy in the treatment of advanced Hodgkin's disease.


The paricipation of phospholipids in the extrinsic pathway of the coagulation process.


Rhythmic heart rate variability (sinus arrhythmia) related to stages of sleep.


Comparison of equivalent spherical volume and aerodynamic diameters for irregularly shaped particles.


Oral fat tolerance studies in chronic pancreatitis.


Gastrointestinal neuropathy in familial amyloidosis. Report of a case with severe diarrhea without steatorrhea or malabsorption.


Microbiological assay of purines.

Microbiological assay of purines.


A rapid and sensitive assay for pyrimidine dimers in DNA.


A method of measuring protein dry weight in solutions of unknown salt concentration and an application to lipoproteins.


Recommendations concerning the use of bacterial collagenase for isolating collagen-associated molecules from tissues.


A sensitive mechanized determination of ATP+ADP.


An enzymatic cycling method for nicotinamide-adenine dinucleotide with malic and alcohol dehydrogenases.


Autoimmune progesterone dermatitis of pregnancy.


The quest for instant mental health.

The quest for instant mental health.


Contraceptive conundrum. II. Hypertension.


An investigation of viral and bacterial agents associated with ovine perinatal mortality.


Australian exotic animal disease control plans.


Inhibition by avidin of the ATP-Pi enchange activities associated with preparations of energy transfer factors A and A-D.


Inability of detect cyclic AMP in vegetative or sporulating cells or dormant spores of Bacillus megaterium.


Irreversible inhibition of ribonucleotide reductase from Ehrlich tumor cells by a modulator analog.


Structure of the flavin site of Chromatium flavocytochrome c -552 .


Substrate specificity of glycogen synthase phosphatase from bovine heart: action on phosphorylase a and histone.


Stability and function of mengovirus RNA in cell-free protein synthesis.


Loss of hepatoma ribosomal RNA during warfarin therapy.


The fully-active nature of synthetic and hydrolytic activities of glucose-6-phosphatase of intact nuclear membrane.


Glucose metabolism in mixed glioblastoma and neuroblastoma cultures.


Kinetics of poliovirus replication in HeLa cells infected by isolated RNA.


Analysis of a crosslinked peptide from calf bone collagen: evidence that hydroxylysyl glycoside participates in the crosslink.


Kinetics of synthesis of cytochromes a and a 3  during respiratory adaptation of yeast.


A study of transfer RNA methylation.

A study of transfer RNA methylation.


Rheologic hysteresis curve shape specificity of mixed lipid films.


K + -stimulated ATPase in purified microsomes of bullfrog oxyntic cells.


Pure and mixed monomolecular films of 12-nitroxide stearate.


SH-groups on the surface of pancreas cells involved in secretin stimulation and glucose-mediated secretion.


Effects of glucagon, 3',5'-AMP and 3',5'-GMP on ion fluxes and transmembrane potential in perfused livers of normal and adrenalectomized rats.


Aminoacyl transfer RNA formation. 3. Mechanism of aminoacylation stimulated by polyamines.


Synthesis and properties of an alternating polydeoxynucleotide containing 4-thiodeoxyuridine: poly (d(A S 4 U)-d(A-S 4 U)).


Preparation and properties of phosphatidal ethanolamine from bovine white matter.


Spin labeled lipid probes in serum lipoproteins.


Two forms of microsomal palmitoyl-coenzyme A synthetase.


The free lipids of Brucella melitensis and Bordetella pertussis.


The kinetics of the redox reactions of ubiquinone related to the electron-transport activity in the respiratory chain.


Liver scintiphotography as an index of liver abnormality.


In vivo neutron activation analysis for calcium in man.


Intravenous perchlorate in brain scanning: effects on choroid plexus and lesion visibility.


Embryonal rhabdomyosarcoma of the head and neck in children.


Frequent association of IgMlambda with crystalline inclusions in chronic lymphatic leukemic lymphocytes.


Herpetic manifestations.

Herpetic manifestations.


Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis. A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.


Genetic control of development in Volvox: isolation and characterization of morphogenetic mutants. Morphogenetic mutants of the colonial green alga, Volvox carteri f. nagariensis, were induced by chemical mutagenesis. The 68 independent mutants are classified into 12 readily identifiable phenotypes affecting various stages of asexual development. Nine of the mutants are temperature sensitive with normal development at 25 degrees and mutant development occurring at 35 degrees . Some mutant genes appear to be involved in the regulation of differentiation or the stability of the differentiated state. Other mutations occur in genes apparently responsible for structural components of dividing cells or adult colonies. Two mutants affect different aspects of the posterior-anterior polarization of the mature colony. One mutation affects a gene which acts very early in colonial development, but affects the appearance of the mature colony. The mutants isolated demonstrate the feasibility of using Volvox to study the genetic control of early steps in embryogenesis.


Sequence homology at the 5'-termini of insect messenger RNAs. Treatment of insect polyribosomes with 1 M KCl released a messenger ribonucleoprotein with a pronounced 16S peak. Phenol extraction resulted in a defined peak of 10S RNA, which was judged as mRNA by the following criteria: it showed specificity for binding to ribosomes, and the formation of initiation complex was dependent on protein initiation factors, GTP, mRNA, and aminoacyl-tRNA. The complex directed protein synthesis upon the addition of elongation factors. mRNA was treated with phosphatase and phosphorylated at the 5'-end with [(32)P]cyanoethylphosphate. [(32)P]mRNA was digested by T1 ribonuclease to completion and chromatographed on DEAE-cellulose. The only fragment with (32)P was 15 nucleotides long; it was treated with pancreatic ribonuclease and fingerprinted. Fractions of AC, AAC, and AAAC were found. Initiation signal AUG or GUG in these mRNAs does not begin immediately at the 5'-end and may be at a distance greater than 15 nucleotides. Alkaline hydrolysis of mRNAs labeled in vivo with [(14)C]adenosine revealed Ap and pppAp. Alkaline hydrolysis of mRNA labeled with (32)P at the 5'-terminus resulted in pAp. Hence, these results suggest that in a heterogeneous population of mRNAs from insects, all start with A and have sequence homology at the 5'-termini. This sequence may reflect the signal for RNA polymerase on the gene or may promote the binding of mRNA to ribosomes.


Cell-free synthesis of procollagen: L-929 fibroblasts as a cellular model for dermatosparaxis. A cell-free system that actively synthesizes collagen was prepared from L-929 fibroblasts. Chromatographic and electrophoretic techniques were used to demonstrate that the only collagenous products are pro alpha(1) and pro alpha(2) chains. The collagen synthesized by the cell-free system was also compared to the collagen extracted from the cells. The cellular collagen was composed of aggregates of pro-alpha chains, while no alpha chains were found. Procollagen peptidase activity could not be detected in the cells, and the activity present in the medium was low, comparable to that in dermatosparaxic cell cultures. These properties indicate that L-929 cells may be a model system for dermatosparaxis.


Stimulation of eukaryotic DNA-dependent RNA polymerase by protein factors. Protein factors that stimulate DNA-dependent RNA polymerase activity in vitro have been purified from Novikoff ascites cells. These factors are not adsorbed by the diethylaminoethylcellulose column used for RNA polymerase purification and appear in the flow-through fraction. They can be fractionated into two classes by chromatography on carboxymethylcellulose. The first peak of activity elutes at 0.1 M NH(4)Cl, is stable to heat treatment of 100 degrees for 5 min, and is designated heat-stable factor; the second peak of activity elutes at 0.3 M NH(4)Cl, is heat labile, and is designated heat-labile factor. Heat-labile factor can be further resolved into two components by chromatography on phosphocellulose. The heat-stable factor and second heat-labile factor stimulate the activity of (Novikoff ascites) RNA polymerase B by several-fold, and appear to function independently. RNA synthesis is stimulated only with native DNA as a template. No stimulation of Escherichia coli RNA polymerase is observed with any of the factors.


Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts. BHK 21/13 fibroblasts grown in the presence of insulin show some characteristics of a transformed strain. Cells broaden, cross each other intricately, and form colonies on both polystyrene dishes and soft agar. The effect of insulin is prevented by addition of N(6)-butyryl adenosine 3':5'-cyclic monophosphate and theophylline to the culture medium. Insulin causes a decrease in the concentration of intracellular adenosine 3':5'-cyclic monophosphate and inhibition of adenylate cyclase activity in BHK fibroblast membranes. This inhibition is observed at insulin concentrations of 0.1 nM or higher.


Orientation and motion of amphiphilic spin labels in hexagonal lipid phases. The acyl chain of spin-labeled fatty acids intercalates between the lipid hydrocarbon chains in hexagonal and micellar phases with the carboxyl group anchored at the lipid water interface. The spectra are characteristic of anisotropic motion and cannot be distinguished from the spectra of these probes in lamellar dispersions. In the hexagonal and micellar phases the molecular motion of the spin label increases as it is moved further away from the carboxyl group, similar to the behavior in the lamellar phase (Jost et al. (1971) J. Mol. Biol. 59, 77-98). The similarity in packing of the acyl chains in the hexagonal and lamellar phases suggests that localized regions of hexagonal phase are compatible with a bilayer matrix.


Pleiotypic control by adenosine 3':5'-cyclic monophosphate: a model for growth control in animal cells. The effects of serum deprivation on several general cellular biochemical processes ("pleiotypic response") related to the growth of normal fibroblasts can be mimicked by treatment of these cells with prostaglandin E(1) in the presence of serum. N(6),O(2)'-Dibutyryl adenosine 3':5'-cyclic monophosphate and theophylline inhibit the membrane transport processes without much effect on other pleiotypic reactions such as overall protein and RNA synthesis and protein degradation. The amount of intracellular cyclic AMP increases during serum starvation and returns to the initial concentration in unstarved cells when growth is initiated again upon addition of serum. Fibroblasts transformed by simian virus 40 have a lower cyclic AMP content than their untransformed parents. Serum deprivation neither increases cyclic AMP content nor significantly affects the pleiotypic reactions in transformed cells. Cycloheximide causes a decrease in cyclic AMP content of normal fibroblasts coincidentally with the ability of this inhibitor to stimulate uridine transport and slow protein degradation in cells deprived of serum.


Host-gene control of type-C RNA tumor virus expression and tumorigenesis in inbred mice. We analyzed the relationship of genetic factors determining the expression of endogenous type-C RNA tumor viruses and other host-gene markers to tumorigenesis. A hybridization experiment was performed with mice of strains AKR/J and C57L, the first filial (F(1)) generation hybrids, the second filial (F(2)) generation hybrids, and the backcrosses to the two parental strains. The results demonstrated a highly significant and predictable association between the expression of complete infectious virus or the viral group-specific (gs) antigen in spleens of young mice and tumorigenesis later in life. Most of the tumors were thymic leukemia and reticulum sarcoma, but other mesenchymal, as well as epithelial, tumors were also observed. Tumors occurred preferentially in gs-antigen- or virus-positive mice of all crosses; in the C57L-backcross and F(2) mice segregating for gs-antigen and virus expression, a few gs-antigen-negative mice developed reticulum cell sarcomas. At the time of their occurrence, the mice were all gs-antigen-positive, and most had virus as well.A minor effect of the major histocompatibility locus, H-2, on leukemogenesis was found in the F(2) mice. Several tumor types were also found that we have never observed in the two parental strains. Our data provide the most direct biological evidence in favor of the viral oncogene theory. Thus, from the presence or absence of expression in early life of splenic gs antigen or virus, we can predict whether or not a tumor is likely to develop later in life. These findings suggest that the genome of endogenous type-C RNA viruses is the major determinant for tumorigenesis although they provide no clues about the factors responsible for the various histological types.


Determination of natural host taxonomy of RNA tumor viruses by molecular hybridization: application to RD-114, a candidate human virus. The lysogenic bacteriophages and the RNA tumor viruses have in common the ability to add their genetic information to the genome of their host cells. The biological similarity extends further; data summarized here indicate that they both possess homology to the DNA of their uninfected indigenous hosts. Sharing of common sequences with normal host DNA has been established with avian, murine, feline, and primate oncornaviruses. This finding provides a method for determining the taxonomic position of the natural host of any new RNA tumor virus isolated. Application of this approach to RD-114 revealed extensive hybridization to normal cat DNA and little, if any, hybridization to human DNA. We conclude that the data assign a feline origin rather than a human origin to RD-114.


A quantitative assay for intercellular adhesion. Intercellular adhesion is measured by a new method based on determination of the rates of attachment of single cells to confluent cell monolayers. The procedure is simple, rapid, and reproducible. Specific and nonspecific intercellular adhesions can be quantitated and distinguished from each other, and from the adhesion to glass (or plastic). The rate of adhesion of single cells to the monolayer is characteristic of more than 80% of the single-cell population. This method, therefore, provides a means for study of the molecular basis of intercellular adhesion.


Conformations of diphosphopyridine coenzymes upon binding to dehydrogenases. The binding of oxidized as well as reduced coenzyme to some dehydrogenases has been studied under different concentration ratios and temperatures by nuclear magnetic resonance spectroscopy. A significant difference in the spectral behavior between DPN(+) and DPNH upon binding is interpreted in terms of fast and slow on-off rates relative to the nuclear magnetic resonance time scale in the binding of these two coenzymes. Significant downfield shifts of DPN(+) were observed upon binding, comparable in magnitude to those expected upon opening (destacking) of the coenzymes in the case of chicken-muscle and lobster-tail lactate dehydrogenase (EC 1.1.1.27) and yeast alchol dehydrogenase (EC 1.1.1.1.). A preliminary survey of several other dehydrogenases is consistent with these findings. In the case of 3-phosphoglyceraldehyde dehydrogenase, there is a possibility that the coenzyme exists in the folded form.


A tumor antigen in tissue cultures derived from patients with Hodgkin's disease. Pellets obtained from supernatant fluids of monolayer cultures of cells from patients with Hodgkin's disease were fractionated by isopycnic density sedimentation. Material in a peak of specific gravity 1.15-1.21 g/ml from two Hodgkin's disease cultures was used to immunize rabbits, and the antisera obtained in this manner were reacted by agar-gel diffusion and immunoelectrophoresis with antigens from the purified peaks and the unfractionated pellets of centrifuged culture medium from all the cultures. The antisera reacted with material from 9 of 10 lines derived from spleens of patients with Hodgkin's disease, 2 of 8 cell lines from histologically negative spleens from patients with Hodgkin's disease and with 3 of 6 lymphoma cell lines not diagnosed as Hodgkin's disease. The antisera did not react with 12 cell cultures prepared from normal adult and fetal spleen and thymus. The antigen from cultures from patients with Hodgkin's disease was not found in material sedimenting at lower specific gravities; it resisted Tweenether solubilization, and migrated as a single band by immunoelectrophoresis. The antigen was not found in disrupted, noncultured tumor cells from patients with Hodgkin's disease, and an antiserum against noncultured, minced tumor tissue did not react with the Hodgkin's disease tissue-culture material. No immunological relationship was found between the tissue culture antigen and Epstein-Barr, RD-114, or Rauscher murine leukemia viruses. The Hodgkin's disease antigen may be a tumorrelated antigen or a component of an oncogenic virus.


Antigenic properties of endogenous type-C viruses from spontaneously transformed clones of BALB-3T3. During long-term tissue culture of spontaneously transformed clones from BALB/c 3T3 mouse-embryo cells, some clones spontaneously begin to produce high titers of endogenous murine type-C viruses. The antigenic properties of these viruses have been analyzed by indirect immunoelectronmicroscopy and can be classified into two distinguishable populations: (a) BALB/c murine myeloma-associated extracellular viruses that carry a specific envelope antigen, xVEA, different from the typical murine leukemia viral envelope antigens; and (b) previously uncharacterized type-C viruses that have neither xVEA nor the murine leukemia viral envelope antigens. The former produces PC1 antigen and the latter might induce a new cell-surface antigen. Neither of these two populations of BALB/3T3 endogenous type-C viruses was able to infect BALB/c cells but both could infect NIH Swiss cells. A single BALB/3T3 clone, then, can release infectious endogenous type-C viruses with at least two different antigenic properties. We conclude that BALB/c somatic cells contain preexisting genetic information for production of at least closely related but, nevertheless, distinct type-C viruses.


The fine structure of human lymph nodes under reactive conditions.


Autonomic defects in paralysis agitans and in the Shy-Drager syndrome.


Cerebrocerebellar pathways. Anatomical data and some functional implications.


Biochemical and pharmacological aspects of Parkinsonism.


Functional aspects of cerebellar signs in clinical neurology.


On the possible role of cyclic AMP in the neuromuscular transmission and in the treatment of myasthenia gravis.


Polyneuropathy caused by unknown universal intracellular deposits-paraamyloidosis? Clinical, light microscopic and electron microscopic study.


Clinical syndromes associated with EB virus infection.


Clinical applications of hypothalamic-releasing factors.


Viral hepatitis.

Viral hepatitis.


Fine structure of acinar cells of human parotid gland.


Metabolic activity during the degenerative and early regenerative stages of minced skeletal muscle.


Regional variations in endoplasmic reticulum in the vas deferens of normal and vasectomized rats.


Tissue explants used for enhancement of herpesvirus isolations. Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made from these same tissue sources. There is also evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.


Purification and properties of yeast histidinol phosphate phosphatase.


Application of laser self-beat spectroscopic technique to the study of solutions of human plasma low-density lipoproteins.


Adenosine 3',5'-monophosphate and protein kinase dependent phosphorylation of ribosomal protein.


Electron paramagnetic resonance study of iodine-induced radicals of benzo( ) pyrene and other polycyclic hydrocarbons.


Photochemical systems of Rhodospirillum rubrum. Light-induced reactions and biological functions of c-type cytochromes in relation to P-870.


Dysfunction patterns analogous to frontal lobe system and caudate nucleus syndromes in some groups of minimal brain dysfunction.


Is anatomy destiny? Panel: the changing role of women psychiatrists.


New Jersey women physicians--their opinions on today's world.


Clinical application of fluorescent staining of chromosomes.


Purification and properties of a mutant bifunctional enzyme from the HisB gene of Salmonella typhimurium.


Studies on the reconstitution of bovine erythrocyte superoxide dismutase. IV. Preparation and some properties of the enzyme in which Co(II) is substituted for Zn(II).


Assembly of the mitochondrial membrane system. IX. Purification, characterization, and subunit structure of yeast and beef cytochrome oxidase.


Assembly of the mitochondrial membrane system. X. Mitochondrial synthesis of three of the subunit proteins of yeast cytochrome oxidase.


Interactions of 3-aminopyridine adenine dinucleotide with dehydrogenases.


Binding of ( 3 H)concanavalin A to normal and transformed cells.


Sialidase activity in transformed cells.


Intraventricular septations complicating neonatal meningitis.


Metastases of glioblastoma multiforme to cervical lymph nodes. Report of two cases.


Gastrointestinal hormones.

Gastrointestinal hormones.


Metabolic aspects of alcohol and their biological significance.


Enhanced release of dopamine  -hydroxylase and norepinephrine from sympathetic nerves by dibutyryl cyclic adenosine 3', 5'-monophosphate and theophylline.


The specific binding of vitamin A acid to equine liver alcohol dehydrogenase.


Mechanism of inactivation of rabbit muscle glyceraldehyde 3-phosphate dehydrogenase by ethacrynic acid.


Increased binding of transfer ribonucleic acid species to ribosomes under conditions interfering with their aminoacylation.


Xanthoma types in relation to the type of hyperlipoproteinemia.


Acrokeratoelastoidosis mimicking palmo-plantar xanthomata.


Experiences with the homozygous cases of familial hypercholesterolemia. A report of 52 patients.


Early diagnosis of familial type II hyperlipoproteinemia.


The role of altered vascular permeability in the induction of experimental xanthomata.


Atherosclerosis of heart valves and the formation of the corneal arcus as models for the study of atherosclerosis.


Advances in lipoprotein research. Biochemical and clinical aspects.


Endocrine profile of a specific prolactin inhibitor: Br-ergocryptine (CB 154). A preliminary report.


The problem-oriented medical synopsis. Applications to patient care, education, and research.


The lysosome: a role in disease.

The lysosome: a role in disease.


Mechanisms of the crystal deposition diseases--gout and pseudogout.


Inositol dehydrogenase from the yeast Cryptococcus melibiosum.


Hereditary sensory neuropathy, type II. Clinical, electrophysiologic, histologic, and biochemical studies of a Quebec kinship.


Evidence for the existence of lactate dehydrogenase X in embryonic chick liver.


The ratio of type-I and -2 Cu(II) in human ceruloplasmin.


Specificity and mode of action of acid carboxypeptidase from Aspergillus saitoi.


Inhibition of homogeneous angiotensin-converting enzyme of rabbit lung by synthetic venom peptides of Bothrops jararaca.


Multiple thoracic nodules in a young asymptomatic woman.


Karyological analysis of Rous sarcoma virus-producing rat-kangaroo cell lines.


Determination of anionic surfactants in water.


Determination of particulate phosphorus by alkaline persulfate digestion.


Analysis of chlorinated aromatic hydrocarbons by exhaustive chlorination: qualitative and structural aspects of the perchloro-derivatives of biphenyl, naphthalene, terphenyl, dibenzofuran, dibenzodioxin and DDE.


Sleep continuity changes in depression.

Sleep continuity changes in depression.


A rapid in vivo test for chromosomal damage.


The mechanism of action of tetanus toxin: effect on synaptic processes and some particular features of toxin binding by the nervous tissue.


Local tetanus in cats: the influence of neuromuscular activity on spinal distribution of  125 I labelled tetanus toxin.


A note on the specific fixation, specific deactivation and non-specific inactivation of bacterial toxins by gangliosides.


Vagal ascent and distribution of  125 I-tetanus toxin after injection into the anterior wall of the stomach.


Ultrastructural changes in the coronet cells of the saccus vasculosus from rainbow trout, Salmo gairdneri (Richardson), kept in sea water.


Prejunctional depressant actions of strychnine on soleus neurotransmission in the cat.


Energy metabolism of working muscle: concentration profiles of selected metabolites.


Sodium depletion and postcaval vein constriction on steroid secretion in the bullfrog.


Specific stimulation and inhibition of renal prostaglandin release by angiotensin analogs.


Differences in macromolecular binding between cyclic AMP and its dibutyryl derivative in vitro.


Cyclic-3', 5'-AMP-dependent and independent protein kinase levels in normal and feline sarcoma virus transformed cells.


Effects of lipids on cyclic-nucleotide phosphodiesterases.


A novel nucleolytic activity associated with rabbit liver tRNA nucleotidyltransferase.


In vitro activation of leukocyte glycogen synthetase.


Storage cells of spleen and bone marrow in thalassemia: an ultrastructural study.


Recurrent Kaposi's varicelliform eruption in Darier's disease.


Steroidogenesis and RNA synthesis in rat adrenal gland in vitro.


Effect of LH on steroidogenesis and cyclic AMP accumulation in rat Leydig cell preparations and mouse tumor Leydig cells.


Carcinoma of the sphenoid sinus.

Carcinoma of the sphenoid sinus.


Plasma LH changes following cortical spreading depression in female rats.


Plasma growth hormone in the anesthetized rat. Effects of dibutyryl cyclic AMP, prostaglandin E 1 , adrenergic agents, vasopressin, chlorpromazine, amphetamine and L-dopa.


Interaction of growth hormone and ACTH in the regulation of adrenocortical secretion in rats.


The action of a human uterine protease on the estrogen receptor.


Mechanism of action of gonadotropin. 3. Binding of adenosine 3',5'-monophosphate by the 105,000 X g supernatant fraction from homogenates of calf ovaries.


TRF and thyroid hormones on prolactin secretion by rat anterior pituitary cells in vitro.


Stimulation of cyclic AMP accumulation in rat ovaries in vitro.


Potentiation by angiotensin II of the vasopressin response to an increasing plasma osmolality.


Intranuclear receptor for androgens in rat epididymis.


Minimal O 2  consumption as an index of thyroid status: standardization of method.


Progesterone and corticosterone binding in rabbit uterine cytosols.


Goat-pox: an anthropozoonosis.

Goat-pox: an anthropozoonosis.


Evoked cortical potentials in experimental spinal cord trauma.


Subacute cerebellar degeneration and Eaton-Lambert syndrome with bronchogenic carcinoma. A case report.


A pleomorphic adenoma (so-called mixed tumor) in the wall of a dentigerous cyst.


Rod (nemaline) bodies in the skeletal muscle of an acute schizophrenic patient.


Considerations on the correlation between the blood groups and the incidence of cerebral tumours.


A study of the relationship of reticuloendotheliosis virus to the avian leukosis-sarcoma complex of viruses.


A temperature-sensitive mutant of Rous sarcoma virus that is defective for replication.


Studies on the mechanism of neuromuscular transmission in insects. The effect of glutamic acid.


Two different activities of alkaline phosphatase in cultured mammalian cells.


The purification of pig vitamin D-induced intestinal calcium binding protein.


Studies on phosphodiesterase from oxyntic cells of bullfrog gastric mucosa. I. Intracellular localization, partial purification, and properties.


Vitamin D 3 -25-hydroxylase: tissue occurrence and apparent lack of regulation.


Covalent attachment of fluorescent groups to the 5'-end of transfer RNA.


Effect of osmolality and phosphate, 'tris', 'tes', 'mes', and 'hepes' hydrogen ion buffers on the motility of bull spermatozoa stored at 37 or 5 degreesC.


A rate-zonal centrifugation procedure for screening particle populations by sequential product recovery utilizing edge-unloading zonal rotors.


A polymeric form of haemoglobin-binding protein in sheep following metabolic and hormonal disturbance.