The degradation of L-histidine in the rat. The formation of imidazolylpyruvate, imidazolyl-lactate and imidazolylpropionate. 1. Soluble and mitochondrial forms of histidine-pyruvate aminotransferase were separated from rat liver preparations by chromatography on DEAE-cellulose. 2. These enzymes were characterized with respect to substrate specificity, substrate affinity, pH optimum, stability and molecular weight by chromatography on Sephadex G-200. 3. Each enzyme has a relatively broad specificity showing significant activity towards l-phenylalanine and l-tyrosine and catalysing transamination with a number of monocarboxylic 2-oxo acids. 2-Oxoglutarate is not a substrate for either enzyme. 4. The molecular weights of the two enzymes, by chromatography on Sephadex G-200, are in the range 130000-150000. 5. The formation in vitro of imidazolyl-lactate from imidazolylpyruvate and NADH was demonstrated by using liver preparations. 6. From a study of imidazolyl-lactate-NAD(+) oxidoreductase activity after electrophoresis of liver preparations on polyacrylamide gel, and from an examination of the activity of l-lactate-NAD(+) oxidoreductase (EC 1.1.1.27) towards imidazolylpyruvate, it is concluded that this latter enzyme is responsible for the formation of imidazolyl-lactate in the liver. 7. Preparations of bacteria obtained from rat faeces form imidazolylpropionate from l-histidine and urocanate without further subculture. The amount of imidazolylpropionate formed is increased under anaerobic conditions and more so in an atmosphere of H(2). It is suggested that the gut flora of the rat contribute largely, if not exclusively, to the formation of imidazolylpropionate normally found in the urine.


Quantitative studies on ferredoxin in greening bean leaves. Two methods of measuring small amounts of the iron-sulphur protein ferredoxin are described. One involves measurements of the signal at g=1.96 produced by reduced ferredoxin in an e.p.r. (electron-paramagnetic-resonance) spectrometer; the other depends on the rate of ferredoxin-dependent electron transport in a chloroplast bioassay measured in an O(2) electrode. These methods of measurement were used to examine the development of ferredoxin during the greening of etiolated bean leaves. Ferredoxin is present in low concentrations in the leaves and cotyledons of 14-day-old etiolated beans (Phaseolus vulgaris L. var. Canadian Wonder), and develops in a linear manner with time when the leaves are illuminated. This synthesis appears to be independent of chlorophyll synthesis during the early stages of greening. However, the chlorophyll/ferredoxin ratio reaches a final value of approx. 360 irrespective of the light intensity, indicating the existence of a control mechanism operative in deciding the stoicheiometry of these components in the mature chloroplast. The ferredoxin synthesis appears to be light-dependent, and red light is the most effective in its promotion. The effect of red illumination is not reversed by far-red light, indicating the absence of a phytochrome control of ferredoxin synthesis. From experiments using specific inhibitors of chloroplast protein synthesis, it is concluded that ferredoxin is synthesized on cytoplasmic ribosomes.


The ultrastructure of Geotrichum candidum hyphae.


Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin. Poly(U)-directed polyphenylalanine synthesis by rat liver ribosomes is strongly inhibited by ricin. Experiments involving hybridization between subunits derived from normal and ricin-treated ribosomes demonstrate that the 60S subunit is the site of action of the toxin. The toxin inactivates the 60S subunit independently of the presence of the 40S subunit.


Recent developments with the L5178Y TK heterozygote mutagen assay system.


Use of the asparagine auxotroph of the L5178Y murine leukemia for the detection of chemical mutagens in vitro and in the host-mediated assay.


The immunodepressive effect of infectious bursal disease virus in chickens.


Investigation of an increase of solid tumors in chickens vaccinated against Marek's disease.


Current status of the host-mediated L5178Y system for detecting chemical mutagens.


Evaluation of chromosome aberration effects of chemicals on mouse germ cells.


Some primary considerations in the interpretation of the dominant-lethal assay.


Chromosome aberrations as a measure of mutagenesis: comparisons in vitro and in vivo and in somatic and germ cells.


Atypical fowlpox in a poultry farm in Illinois.


New developments in mutagenicity screening techniques with yeast.


Estrogen target cells in the skin.

Estrogen target cells in the skin.


Strategies for developing multisource funding for community mental health centers.


Scanning electron microscopic study of normal and molar trophoblast.


Gynecologic aspects of breast disease.

Gynecologic aspects of breast disease.


A process to help community mental health boards plan programs and facilities.


Myomycin, a new antibiotic.

Myomycin, a new antibiotic.


Surgical correction of islet cell adenocarcinoma in a dog.


Ultrastructure of the crayfish kidney--coelomosac, labyrinth, nephridial canal.


Virology and the nonhuman primate.

Virology and the nonhuman primate.


Editorial: The kidney and uric acid excretion in man.


Cell culture techniques in the search for cancer viruses of man.


Cell transformation by viruses. I. Significance of virus-specific antigens induced by deoxyribonucleic acid-containing tumor viruses. II. Complementary ribonucleic acid in cells transformed by ribonucleic acid-containing tumor viruses.


Use of nonhuman primates in virus research.


Activities of the NIH-WHO regional reference center for simian viruses.


Natural and experimental infections of nonhuman primates with respiratory viruses.


Viral epizootics in captive nonhuman primates.


Biochemical aspects of viral transformation.


Spontaneous lymphoma of the nonhuman primate.


C-type oncogenic viruses of nonhuman primates.


Virus from stumptailed monkey (Macaca arctoides) kidney cultures.


Pathology and clinical history of Delta herpesvirus infection in patas monkeys.


Recent studies on the isolation and characterization of Delta herpesvirus.


Ascorbic acid (vitamin C) and its effects on parainfluenza type 3 virus infection in cotton-topped marmosets.


Spontaneous neoplasms of nonhuman primates. Attempted transmission to immunosuppressed hosts and in vitro characterization.


Comparative protection by polyoma virus and xenogenic polyoma-induced tumour cells against tumour allograft.


Use of oral antibiotics in protected units environment: clinical effectiveness and role in the emergence of antibiotic-resistant strains.


Clinicopathology of equine rhinopneumonitis abortion in central Iowa.


The effects of circumcision on sleep-wake states in human neonates.


Studies on nucleic acids in RSV-induced mouse ascites sarcoma cells. Base compositions and chromatographic behaviors.


Adenosine 3', 5'-monophosphate-dependent protein kinase from SV 40-induced hamster tumor.


Immunofluorescent and electron microscopic studies of cultured human cells successively transformed by Rous sarcoma virus and simian virus 40.


Molecular forms and activities of glucose 6-phosphate dehydrogenase in cultured human cells.


Immunologic deficiency in Hodgkin's disease.


Human immunoglobulin M antibody in the sero-diagnosis of Japanese encephalitis virus infections.


Family-based epidemiologic studies. The second Wade Hampton Frost Lecture.


Evolutionary aspects of interactions between human host and virus populations.


Epidemic of adenovirus type 7 acute conjunctivitis in swimmers.


Enzymatic production of experimental emphysema in the dog. Route of exposure.


Electron microscopic studies on the parafollicular cells and parafollicular cell complexes in the dog.


The central nervous system, liver, and spleen of FM mice. Ultrastructural, histochemical, and biochemical studies.


Action of micrococcal nuclease on chemically modified deoxyribonucleic acid.


Studies on the relationship between deoxyribonucleic acid polymerase activity and intracisternal A-type particles in mouse myeloma.


Differences in in vivo methylation patterns of tyrosine and histidine transfer ribonucleic acids from rat liver and Novikoff hepatoma.


Isolation and partial characterization of chimpanzee plasma high density lipoproteins and their apolipoproteins.


Chemical structure of a novel glycolipid from an extreme thermophile, Flavobacterium thermophilum.


Frequency and temperature dependence of the proton relaxation rates of solvent and substrate interaction with isocitrate dehydrogenase bound Mn(II).


A comparison of the kinetics and stoichiometry of proton uptake with aldehyde reduction for liver alcohol dehydrogenase under single turnover conditions.


Biosynthesis of viomycin. I. Origin of alpha, beta-diaminopropionic acid and serine.


Electron and nuclear magnetic resonance studies of the interaction of pyruvate with transcarboxylase.


Circular dichroism spectra of the copper enzyme, galactose oxidase, in the presence of its substrates and products.


Guanidine hydrochloride and acid denaturation of horse, cow, and Candida krusei cytochromes c.


Biosynthesis of viomycin. II. Origin of beta-lysine and viomycidine.


Duck hepatitis virus interactions with DDT and dieldrin in adult mallards.


PPD-stimulated interferon: in vitro macrophage-lymphocyte interaction in the production of a mediator of cellular immunity.


Derivative polarography of normal and cancerous tissue extracts and urines.


Hormonal regulation of the immune response. II. Influence of pituitary and adrenal activity on immune responsiveness in vitro.


Investigations on the specificity of hormone receptors in planarians.


Structure and function of the transplanted pituitary in the seawater goby, Gillichthys mirabilis. I. The rostral pars distalis.


Enzymatic ADP-ribosylation of proteins and regulation of cellular activity.


Relationship of craniofacial changes and skeletal age increments in females.


A longitudinal examination of speed of reaction and speed of movement in young boys 7 to 13 years.


Hepatoblastoma: pathological studies in a case.


Neural control of pituitary adrenal activity in Cyprinus carpio.


The phosphorescent state of indole. Observations using optically detected magnetic resonance.


Resolution of tryptophan phosphorescence from multiple sites in proteins using optical detection of magnetic resonance.


Collaborative study of an on-column periodate reaction method for the analysis of phenylpropanolamine hydrochloride in elixirs.


Crystal structure of 1-(2-indol-3-ylethyl)-3-carbamidopyridinium chloride, an intramolecular model of the nicotinamide adenine dinucleotide--tryptophan charge-transfer complex.


Fructose-1, 6-bisphosphatase of an extreme thermophile.


Electron and proton transport in Rhodospirillum rubrum chromatophores.


Sparganum proliferum, a sparganum infected with a virus?


Effect of lysolecithin and albumin on lecithin-cholesterol acyltransferase activity in human plasma.


Isospora felis: development in cultured cells with some cytological observations.


Hemoglobin absorption in the gut of a monogenetic Trematode, Dicilidophora merlangi.


Hyperlipidemia and coronary artery disease. Prinicples of diet and drug treatment.


Anucleate mammalian cells: applications in cell biology and virology.


HeLa cell plasma membranes.

HeLa cell plasma membranes.


Use and misuse of steroids.

Use and misuse of steroids.


The role of macromolecules in neuronal function in Aplysia.


Reiter's disease: a review with special attention to cardiovascular and neurologic sequellae.


Some aspects of the penicillin V-acylase produced by Rhodotorula glutinis var. glutinis.


Preparation and evaluation of ACTH antibodies.


Arterial hypertension induced by 9 alpha-fluorocortisol in rats.


Sex dimorphism in the adrenal of hamsters.


Ultrastructural pathology of murine cytomegalovirus infection in cultured mouse nervous system tissue. Mouse spinal cord-ganglia cultures were innoculated with murine cytomegalo-virus 14 days after explantation. Intranuclear virus was first observed 4 days after infection. The viruses, which occurred in four forms, were observed in increasing numbers during the ensuing 4 days. Differences were noted in the relative prevalence of certain of these forms in older as compared to younger cultures. This suggests that variations in virus form are related to virus maturation. Cytoplasmic viruses were occasionally observed, but their site of origin is not certain. A variety of cytoplasmic inclusions were seen, particularly in the older cultures. It seems likely that they represent specific cell responses to the presence of the virus. They were not observed in the control cultures, even though some of the latter did show severe degenerative changes.


Ultraviolet light and cytoplasmic tubules in lupus erythematosus.


Chordoma-like subcutaneous nodules in an infant.


Proceedings: Cylindroma.

Proceedings: Cylindroma.


The genetics of herpesvirus.

The genetics of herpesvirus.


Light microscopic observations of transplantable mouse hepatomas.


A direct relationship between immune competence and the subcutaneous growth rate of a malignant murine lung tumor.


Nucleotide contents of ascites hepatoma cells and their changes induced by D-galactosamine.


Prolactin receptors in mammary carcinoma cells.


The enzymology of methionine metabolism in rat hepatomas.


Sites of synthesis of murine RNA tumor virus (oncornavirus) group-specific antigens.


Strain- and age-dependent transplacental carcinogenesis by 1-ethyl-1-nitrosourea in inbred strains of mice.


Sensitivity of transformed and nontransformed cells to amphotericin B and several rifamycin derivatives.


Thioacetamide-induced alterations in nuclear RNA transport.


The temperature dependency of cochlear adaptation and masking in the guinea pig.


Mitochondrial membrane-associated properties of Morris hepatomas.


The cryptophthalmos-syndactyly syndrome. Description, manner of inheritance, and notes on the eye lesions.


Studies on the relationship between infection with bovine C-type virus, leukemia, and persistent lymphocytosis in cattle.


Membranous conjunctivitis due to adenovirus type 7 infection.


Schistosoma mansoni: ultrastructural observations on the small intestine of murine host.


Ectopic ACTH production in carcinoma of the lung. Immunoreactive ACTH was found in almost all tissue extracts of lung carcinoma from patients without clinical evidence of Cushing's syndrome; i.e. 14 of 15 primary tumors, nine of nine metastatic lymph nodes, and four of four metastatic liver nodules contained immunoreactive ACTH. The incidence of ACTH in extracts of other tumor types was much lower. Comparable normal tissues contained no detectable ACTH. Immunoreactive growth hormone, parathyroid hormone, or gastrin was not found in the same carcinoma tissue. The predominant form of ACTH in the tumor extracts was big ACTH. In pituitary extracts little ACTH predominated.53% of 83 patients with lung carcinoma had afternoon plasma ACTH levels greater than 150 pg/ml; more than 90% of plasmas containing less than 150 pg/ml were obtained from patients who had received radiation therapy or chemotherapy. 31% of 45 patients with chronic obstructive pulmonary disease (COPD), 28% of 25 patients with other severe lung disease, and 6% of 33 controls had elevated values. Big ACTH predominated in the plasma of patients with lung carcinoma or COPD having elevated ACTH levels. Tissue from the lung of a smoking dog with atypical histologic changes contained immunoreactive ACTH, almost exclusively in the big form, while tissue from another smoking dog that was histologically normal contained no ACTH. Thus ACTH may be present even in precancerous lung lesions. These studies suggest that serial plasma ACTH levels may be of value in screening for, and/or management of, patients with carcinoma of the lung.


Beta-sitosterolemia and xanthomatosis. A newly described lipid storage disease in two sisters. Although the usual diet may contain 150-250 mg of plant sterols, chiefly beta-sitosterol, only trace amounts of these sterols have heretofore been found in human or animal blood and tissues. We now report elevated plant sterol levels in the blood and tissues of two sisters with extensive tendon xanthomas but normal plasma cholesterol levels. Besides beta-sitosterolemia and xanthomatosis, no other physical, mental, or biochemical abnormalities were detected.Repeatedly, the plasmas of the two sisters have contained 27.1 and 17.7 mg/100 ml of beta-sitosterol, 9.7 and 8.2 mg/100 ml of campesterol, and 0.5 and 0.5 mg/100 ml of stigmasterol, respectively. These plant sterols constituted 15.6 and 11.3% of the total plasma sterols. Some 60% of the plasma beta-sitosterol and campesterol was esterified; the measurable stigmasterol was entirely unesterified. The transport of the plasma beta-sitosterol and campesterol was largely in low density lipoproteins (76 and 83%, respectively). High density lipoproteins carried the remainder. Plant sterols were barely detectable in the very low density lipoprotein fraction. Only trace amounts of stigmasterol could be detected in the low density and high density lipoprotein fractions. The plant sterol content of the red blood cells averaged 12-13 mg/100 ml packed cells or about 13% of the total sterols. Two tendon xanthoma biopsies with the usual high concentration of cholesterol had 36.7 and 4.0 mg of plant sterols/g dry wt, of which 25.7 and 2.9 mg were beta-sitosterol, entirely in the free form. Plant sterols were also found in adipose tissue (0.2 mg/g wet wt) and in skin surface lipids (3.2 mg/g of lipid). The intestinal absorption of beta-sitosterol in both the patients, measured by two techniques, indicated greatly increased absorption of this sterol (about 24 and 28% in the patients L. H. and R. H., respectively, normal absorption being <5%). We suggest that increased absorption of beta-sitosterol must be considered as one cause of this disease. The reason for the extensive xanthomatosis in these two patients remains unknown. Perhaps in some way plant sterols initiated the development of xanthomas with otherwise normal plasma cholesterol levels. Clinical atherosclerosis has not yet occurred. The occurrence of beta-sitosterolemia in these two sisters with un-affected parents suggests an inherited recessive trait.


The mechanism of activation of hormone-sensitive lipase in human adipose tissue. A partially purified hormone-sensitive triglyceride lipase of human adipose tissue was found to be activated twofold by the addition of cyclic 3',5'-AMP, ATP, and magnesium ions. Lipase activities against diolein and monoolein were not affected. Addition of protein kinase inhibitor at zero time completely inhibited activation, and this inhibition was prevented by prior addition of an excess of exogenous protein kinase (from rabbit skeletal muscle). Addition of protein kinase inhibitor during the activation step blocked the activation process without a time lag, suggesting that protein kinase operates directly on hormone-sensitive lipase. Further purification yielded a fraction free of protein kinase, and lipase activation in this fraction depended absolutely on addition of exogenous kinase. Incubation of human fat with epinephrine or isoproterenol stimulated lipolysis and caused conversion of nonactivated hormone-sensitive lipase to its activated form, as indicated by a decrease in the activation subsequently obtainable in fractions prepared from such hormone-treated tissues. These findings strongly suggest that the stimulation of lipolysis by hormonal treatment is the consequence of the activation of hormone-sensitive triglyceride lipase by cyclic 3',5'-AMP-dependent protein kinase.


On the mechanism of lithium-induced diabetes insipidus in man and the rat. The mechanism of lithium-induced diabetes insipidus was investigated in 96 patients and in a rat model. Polydipsia was reported by 40% and polyuria (more than 3 liter/day) by 12% of patients receiving lithium. Maximum concentrating ability after dehydration and vasopressin was markedly impaired in 10 polyuric patients and was reduced in 7 of 10 nonpolyuric patients studied before and during lithium therapy. Severe polyuria (more than 6 liter/day) was unresponsive to trials of vasopressin and chlorpropamide, but improved on chlorothiazide. Rats receiving lithium (3-4 meq/kg/day) developed massive polyuria that was resistant to vasopressin, in comparison to rats with comparable polyuria induced by drinking glucose. Analysis of renal tissue in rats with lithium polyuria showed progressive increase in the concentration of lithium from cortex to papilla with a 2.9-fold corticopapillary gradient for lithium. The normal corticopapillary gradient for sodium was not reduced by lithium treatment. The polyuria was not interrupted by brief intravenous doses of vasopressin (5-10 mU/kg) or dibutyryl cyclic AMP (10-15 mg/kg) capable of reversing water diuresis in normal and hypothalamic diabetes insipidus rats (Brattleboro strain). The present studies suggest that nephrogenic diabetes insipidus is a common finding after lithium treatment and results in part from interference with the mediation of vasopressin at a step distal to the formation of 3',5' cyclic AMP.


A specific role for saline or the sodium ion in the regulation of renin and aldosterone secretion. It is well established that in normal man the renin-angiotensin-aldosterone system is responsive to changes in volume. The present study was performed to determine whether sodium has an action apart from volume in the regulation of the secretion of renin and aldosterone. Acute volume expansion was induced either by saline, dextran, or glucose infusion in supine, normal subjects in balance on a 10 meq sodium/100 meq potassium diet. Plasma renin activity (PRA), angiotensin II (A II), aldosterone (PA), cortisol, serum sodium, and potassium were measured every 10 min for the first 30 min and then at 1, 2, 4, 6, and 8 h. During saline infusion (500 cm(3)/h for 6 h) mean PRA and A II levels declined very rapidly, falling significantly below control at 10 min (P < 0.01) and by 50% at 60 min. Thereafter, the rate of fall was more gradual, reaching a nadir at 360 min (70-80% below control). PA declined in a parallel pattern except that a significant fall did not occur until 30 min. In contrast to saline, dextran infusion (250 cm(3)/h for 4 h) did not produce a significant fall in PRA, A II, or PA until 4 h after the start of the infusion despite equivalent volume expansion. On the other hand, the infusion of 5% glucose and water (500 cm(3)/h for 6 h) did not produce a significant decline in PRA, A II, or PA over the first 6 h of the study. Although the response rate of PRA, A II, and PA was different in each of the three infusion studies, these parameters were significantly correlated within each study. Serum sodium and potassium levels did not change during any study except dextran infusion, where a significant fall in both occurred at 120 min. In all the infusion studies, plasma cortisol levels gradually declined during the 8-h study period consistent with its expected rhythm of diurnal secretion. These results demonstrate that rate of response of the renin-angiotensin-aldosterone system to acute volume expansion with saline differed from that with dextran and glucose infusion in sodium-depleted man. The data support a specific role for volume expansion with saline or the sodium ion per se in the regulation of renin and aldosterone.


Genetic control of the immune response: the effect of non-H-2 linked genes on antibody production.


Studies on the effect of specific antisera on the metabolism of cellular antigens. II. The synthesis and degradation of TL antigens of mouse cells in the presence of TL antiserum.


Sequence of events mediating the effect of cholera toxin on rat thymocytes. We have found that in rat thymocytes binding of [(125)I]choleragen is followed by cellular accumulation of cyclic 3',5'-AMP which, in turn, is followed by stimulation of amino acid transport. Binding of cholera toxin was complete by 30 min and remained constant for the subsequent 150 min. After stimulation by choleragen, cellular cyclic 3',5'-AMP became maximal by 30 min, after which it declined steadily so that by 90 min of incubation, cellular cyclic nucleotide levels were only 20% of those seen at 30 min. Stimulation of amino acid transport, although detectable by 15 min, did not become maximal until 120 min (by which time cellular cyclic 3',5'-AMP had decreased by more than 80%). We have also used this system to delineate the step at which various pharmacologic agents and hormones act to alter the sequence of events mediating the response of rat thymocytes to cholera toxin. The ability of cycloheximide to abolish choleragen-stimulated amino acid influx without reducing [(125)I]choleragen binding or cellular cyclic 3',5'-AMP suggests that cyclic nucleotide stimulation of amino acid transport includes a step involving protein synthesis.


Comparative in vitro studies on effector cell diversity in the cellular immune response to murine sarcoma virus (MSV)-induced tumors in mice.


The characteristics of a circulating antigen in schistosomiasis.


Metabolic bone disease--recent developments.


Provocative tests of the hypothalamic-anterior pituitary-target organ axis.


The enterohormones. Current status and review.


Cancer and ectopic hormones.

Cancer and ectopic hormones.


Multidisciplinary teams develop programming for patient education.


Biochemistry of cancer cells: focus on the cell surface.


Toxoplasmosis: a review.

Toxoplasmosis: a review.


Nucleus gracilis responses to knee joint motion: a frequency response study.


Statistical properties of neurons in the rat mesencephalic reticular formation.


Reward-associated inhibition and pain-associated excitation lasting seconds in single intralaminar thalamic units.


Presynaptic depolarization and extracellular potassium in the cat lateral geniculate nucleus.


Hippocampal suppression of intralaminar thalamic unit responses in cats and comparison with suppression evoked from the caudate nucleus.


Dissecting a short-term memory circuit in the rat brain. I. Changes in entorhinal unit activity and responsiveness of hippocampal units in the process of classical conditioning.


Discharge characteristics of neurons in anteroventral and dorsal cochlear nuclei of cat.


Repetitive discharge rate of a simple neuron model with accumulation of after-hyperpolarization conductance.


Spontaneous activity of single neurons in the precruciate area of the cat's sensory motor cortex.


Effects of excitants on neurones and cerebellar-evoked field potentials in the inferior olivary complex of the rat.


Responses of spinal cord neurons to graded noxious and non-noxious stimuli.


Correlation of latency to drink and unit activity following cholinergic stimulation of medial septal nucleus in rats.


Distribution of carotid body chemoreceptor afferents in the medulla of the cat.


Projection of tooth pulp afferents in the brain stem of rhesus monkey.


Caudate-evoked inhibition and actions of GABA and other substances on cat pallidal neurons.


Depression by p-methoxyphenylethylamine of sympathetic reflex firing elicited by electrical stimulation of the carotid sinus nerve or pelvic nerve.


The effect of intracochlearly administered acetylcholine-blocking agents on the efferent synapses of the cochlea.


Somatosensory properties of neurons in the superior parietal cortex (area 5) of the rhesus monkey.


Effect of gamma rays and some chemical mutagens on induction of telotrisomics in Hordeum.


The repair of methyl methanesulphonate-induced lesions in the DNA of a murine lymphoma cell.


Separation and characterization of polypeptides from the venom of Dendroaspis viridis.


Visual and somatosensory receptive fields of neurons in the squirrel monkey pulvinar.


Hormonal regulation of cyclic-AMP-dependent protein kinase of rat diaphragm by epinephrine and insulin.


Thiophosphate-analogues and 1-N-oxides of ATP and ADP in mitochondrial translocation and phosphoryl-transfer reactions.


Preparation of the enzymatically active subfragment of myosin by proteolysis of myofibrils.


Studies of glutamate dehydrogenase. The interaction of glutamate dehydrogenase with alpha-NADH.


Correlations between structure and spectroscopic properties in membrane model system. Fluorescence and circular dichroism of the cytochrome c-cardiolipin system.


Subunit structure of aspartokinase 3 of Escherichia coli K12.


Studies on thymus products. VI. The effects of cyclic nucleotides and prostaglandins on rosette-forming cells. Interactions with thymic factor.


Cyclic AMP-mediated stimulation by calcium of thymocyte proliferation.


Control of DNA replication in Physarum polycephalum. I. Specific activity of NAD pyrophosphorylase in isolated nuclei during the cell cycle.


Mutant human fibroblast clones able to grow on homocysteine instead of cyst(e)ine.


Depression of RNA synthesis in the prematurely condensed chromatin of pulverized HeLa cells.


Immune microfluorimetric analysis of the distribution of species specific nuclear antigens in HeLa-chick erythrocyte heterokaryons.


Radiation-induced DNA synthesis in nuclei of hen erythrocytes reactivated in heterokaryons.


Comparative activities of mammalian, reptilian, and piscine gonadotropins in monkey granulosa cell cultures.


The control of adrenocortical secretion in the brush-tailed possum, Trichosurus vulpecula.


Sleep induced by spinal cord cholinergic stimulation.


Remission of acanthosis nigricans associated with polycystic ovarian disease and a stromal luteoma.


Ovarian maintenance in the hypophysectomized catfish, Heteropneustes fossilis (Bloch), with mammalian hypophyseal and placental hormones, and gonadal and adrenocortical steroids.


Secretion and metabolic efficacy of parthyroid hormone in patients with severe hypomagnesemia.


Steroid production from plasma cholesterol. II. In vivo conversion of plasma cholesterol to ovarian progesterone and adrenal C19 and C21 steroids in humans.


Integrated concentrations of growth hormone correlated with stage of puberty and estrogen levels in girls.


Uptake of iodinated human chorionic gonadotropin by monkey ovaries in vivo.


Pregnenolone in man: plasma levels in states of normal and abnormal steroidogenesis.


Protection by pregnancy. Zollinger-Ellison syndrome.


Ascorbate oxidase. New method of purification of the enzyme from green zucchini squash and identity of its copper moiety with that of cucumber ascorbate oxidase.


Extracellular current density analysis of responses in cerebellar cortex to climbing fiber activation.


Local vesicle populations in rat superior cervical ganglia and the vesicle hypothesis.


Evidence that cyclic AMP is not the chemical mediator of the metabolic changes accompanying phagocytosis.


The relationship between ATP and an electrogenic pump in the plasma membrane of Neurospora crassa.


Some inferences from galactokinase deficiency.


The pathogenesis of anuria following shock.


Galactokinase deficiency in twins: clinical and biochemical studies.


Infectious mononucleosis and Epstein-Barr virus in childhood.


"Typus Edinburgensis"?

"Typus Edinburgensis"?


Multiple activities of DNA polymerase from visna virus.


The association of herpesvirus type 2 infection and cervical cancer.


Genetic economy of polyoma virus: capsid proteins are cleavage products of same viral gene. Two-dimensional tryptic peptide maps of the nonhistone proteins of purified polyoma virus show marked similarities. Protein P(1) is a nondisaggregated, possibly covalent, dimer of the major capsid protein P(2), whereas P(3) and P(4) share several new peptides as well as many of the peptides derived from P(2). Extensive use of this kind of processing of viral proteins during the biosynthesis of DNA-containing animal viruses has not been reported previously.


Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein. Extracts of wheat germ are capable of synthesizing the major capsid protein of simian virus 40. Poly(A)-containing RNA from BS-C-1 cells infected with simian virus 40 directed the synthesis of a novel polypeptide that migrates in polyacrylamide gels together with the major capsid polypeptide of simian virus 40, VP-1. The patterns of the major tryptic peptides of purified VP-1 and the novel polypeptide synthesized in vitro were identical after two-dimensional paper electrophoresis. The novel polypeptide was not synthesized in response to poly(A)-rich RNA from uninfected cells or from virus-infected cells treated with cytosine arabinoside. Messenger RNA from infected cells purified by selective hybridization to DNA of simian virus 40 directs the synthesis of a major polypeptide of electrophoretic mobility similar to that of VP-1 of simian virus 40. This approach should prove useful in identifying additional products specified by DNA tumor viruses.


The influence of N6-2'-O-dibutyryl cyclic 3',5'-AMP on the metabolism of cholesterol-26-14C and cholesterol-4-14C in the rats fed on atherogenic or lipid-rich diet.


Specific binding of cholera toxin to isolated intestinal microvillous membranes. A sucrose density gradient assay was used to demonstrate the specificity and saturation of the binding of [(125)I]cholera toxin to isolated intestinal microvillous membranes from rat small intestine. When the toxin is first complexed to antitoxin and then exposed to intestinal membranes, the binding of cholera toxin is inhibited. To emphasize the physiologic importance of these observations, similar concentrations of [(125)I]cholera toxin were shown to stimulate the accumulation of cyclic AMP in mucosal homogenates and to increase the secretion of fluid into intestinal loops, whereas the same concentrations of toxin mixed with antitoxin had no effect on cyclic AMP accumulation. These studies suggest that cholera toxin attaches to brush border binding sites before exerting its biologic effect and that local intestinal antibody protection against cholera toxin may be due to inhibition of toxin attachment to these binding sites.


Inhibition of induced aldosterone biosynthesis with a specific antagonist of angiotensin II. The present study determined the effect of [Sar(1)-Ile(8)]-angiotensin II on steroidogenesis and induced aldosterone synthesis by the octapeptide angiotensin II, the heptapeptide des-Asp(1)-angiotensin II, and adrenocorticotropic hormone. Rabbit adrenal cells were suspended by incubation of the capsular cortical tissue with collagenase, and aldosterone levels were determined by immunoassay. Steroidogenic responses to angiotensin II and des-Asp(1)-angiotensin were essentially the same. [Sar(1)-Ile(8)]-angiotensin II (10(-7) to 5 x 10(-7) M) had no significant effect on basal aldosterone biosynthesis. However, when added with steroidogenic peptides, it completely blocked the effect of angiotensin II and des-Asp(1)-angiotensin II but not of adrenocorticotropic hormone. The dose ratio of the antagonist to angiotensin II that gave 100% inhibition was about 2:1 and to des-Asp(1)-angiotensin II, about 50:1. The data suggest that des-Asp(1)-angiotensin II has a much higher affinity for the angiotensin receptor in adrenal cortex than angiotensin II.


Homology between Burkitt herpes viral DNA and DNA in continuous lymphoblastoid cells from patients with infectious mononucleosis. At least 90% of the sequences of purified, in vitro labeled, DNA from Epstein-Barr virus (prepared from HR-1, Burkitt's lymphoblastoid cells) are homologous to the DNA of the herpes virus contained in cell lines derived from patients with infectious mononucleosis. The thermal stability of the homologous and heterologous hybrid DNA molecules could not be differentiated, indicating at least 97% matching of base pairs between DNA of Epstein-Barr virus and the herpes viral DNA contained in the lymphoblasts from patients with infectious mononucleosis.


Nucleotide sequences of RNA transcribed in infected cells and by Escherichia coli RNA polymerase from a segment of simian virus 40 DNA. The nucleotide sequence of 180 residues of an RNA transcript of DNA of simian virus 40 has been deduced. This sequence adjoins a preferred initiation site for E. coli RNA polymerase. Comparison of this sequence with that of complementary RNA of simian virus 40 from infected cells has shown that this site also adjoins the apparent 3' terminus of some of the cytoplasmic complementary RNA of simian virus 40.


Absence of a specific ganglioside galactosyltransferase in mouse cells transformed by murine sarcoma virus. The ganglioside composition of a nonproducer subclone (derived from BALB/c 3T3 mouseembryo cells) transformed by Kirsten murine sarcoma virus was drastically altered compared to the nontransformed parental clone. The transformed clone was unable to synthesize the mono- and disialogangliosides, G(M1) and G(D1a), due to the complete absence of a specific galactosyltransferase. The lack of this enzyme activity was established by sensitive radiochemical and enzymological techniques in vivo and in vitro.


Translational diffusion coefficient and partition coefficient of a spin-labeled solute in lecithin bilayer membranes. The translational diffusion coefficient and the partition coefficient of a spin-labeled solute, di-t-butyl nitroxide, in an aqueous suspension of dipalmitoyl lecithin vesicles have been studied by electron spin resonance spectroscopy. When the lecithin is cooled through its phase transition temperature near 41 degrees C, some solute is "frozen out" of the bilayer, and the standard partial molar enthalpy and entropy of partition go more positive by a factor of 8 and 6, respectively. However, the apparent diffusion constant in the lecithin phase is only slightly smaller than that in water, both above and below the transition temperature. The fraction of bilayer volume within which solute is distributed may increase with temperature, contributing to the positive enthalpy of partition. Comparison of time constants suggests that there is a permeability barrier to this solute in the periphery of the bilayer.


Initiation characteristics for the synthesis of five T4 phage-specific messenger RNAs in vitro. The involvement of the nucleoside triphosphates in the initiation of the synthesis of the messenger ribonucleic acid of five T4 specific enzymes has been studied. Only one of these, the messenger RNA for deoxynucleosidemonophosphate kinase, can be initiated in the presence of one nucleoside triphosphate, namely ATP. All of the remaining four require the presence of at least two nucleoside triphosphates during the initiation period. The combination of ATP and UTP was best for the initiation of messenger RNA for dihydrofolate reductase, ATP and CTP for deoxycytidylate hydroxymethyltransferase and beta-glucosyltransferase, and ATP and GTP for alpha-glucosyltransferase. We have concluded that there is a great variation in the nucleotide composition and sequence of the initiation sites in T4 DNA. No correlation in the requirements of nucleoside triphosphates during the initiation period could be observed among the five systems studied according to their classification as one type or another of "early" T4 messenger RNA.


A comparison of membrane proteins of normal and transformed cells by lactoperoxidase labeling. The enzyme lactoperoxidase (iodide:hydrogen-peroxide oxidoreductase, EC 1.11.1.8) was used to iodinate ((125)I) accessible proteins on membranes of intact virally transformed and untransformed cells. A number of labeled bands of proteins were detected by acrylamide gel electrophoresis. A heavily labeled band with a molecular weight of approximately 250,000 daltons was found in all untransformed cells but was absent from transformed cells. When Coomassie-blue-stained membrane preparations were compared, a band was seen in normal cells which co-migrated with the lactoperoxidase-labeled band. In transformed cell membranes, three discrete bands were present in the same position. Thus, the expression of this protein may be altered when cells are in the transformed state.


Multiple regulation of the galactose operon-genetic evidence for a distinct site in the galactose operon that responds to capR gene regulation in Escherichia coli K-12. Previous results demonstrated that the capR (lon) locus, which is not linked to the gal operon, independently controls the synthesis of the gal operon enzymes and gal mRNA, i.e., galO(+)capR9 strains are derepressed 4- to 6-fold as compared to galO(+)capR(+) strains. A mutation has been isolated and localized in the galactose operator region that defines a new and distinct site of control. Mutation in this site, designated galO(capR+), causes a 4-fold increase in the galactose enzymes, galactokinase (EC 2.7.1.6) and UDP-galactose-4-epimerase (EC 5.1.3.2), in a capR(+) background. These mutants exhibit a reduced response to regulation by the unlinked regulator gene capR (lon). However, the galO(capR+) mutants are still subject to control by the galR repressor, since they can be further derepressed by growth in the presence of D-fucose. They also synthesize more galactokinase when grown in glycerol as compared to glucose. Thus there are now at least three, and probably four, sites for control of mRNA synthesis in the operator-promoter regions of the gal operon, making it one of the most complex control systems to date for a single operon in bacteria. The complexity is sufficient to accommodate models for differentiation in higher organisms that require more than one "switch" to control a single group of genes.


Homeoviscous adaptation--a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. E. coli incorporates increasing proportions of saturated and long-chain fatty acids into phospholipids as growth temperature is increased. It was found that this compositional variation results in the biosynthesis of phospholipids that have identical viscosities at the temperature of growth of the cells. This "homeoviscous adaptation" can also be observed in E. coli membrane preparations. Viscosities were determined by use of the electron spin resonance spin-label technique.


Effect of prostaglandins and epinephrine on the level of cyclic AMP-14C of smooth muscle from the rhesus monkey.


The effect of light on fruiting body formation and adenosine 3':5'-cyclic monophosphate metabolism in Coprinus macrorhizus. The monokaryotic mycelia of a mutant strain, fis(c), of Coprinus macrorhizus, which are able to form monokaryotic fruiting bodies in the light, failed to form any fruiting bodies in darkness. A dikaryon and a mutant strain, ds, formed malformed fruiting bodies in darkness. Illumination for 1 day of fis(c) mycelia grown in darkness for 4 days or longer was effective in inducing malformed fruiting bodies. The accumulation of adenosine 3':5'-cyclic monophosphate in the illuminated mycelia of strain fis(c) was demonstrated. The illuminated mycelia of strain fis(c) produced high levels of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] and phosphodiesterase (3':5'-cyclic-AMP 5'-nucleotidohydrolase, EC 3.1.4.17), which degrades cAMP, while the dark-grown mycelia showed no or very low activities of these enzymes. Dikaryotic mycelia and monokaryotic mycelia of strain ds produced significant amounts of these enzymes even in darkness. When the dark-grown mycelia of strain fis(c) were exposed to continuous light, the activities of adenylate cyclase and phosphodiesterase increased rapidly after a lag period whose length depends on the culture age of mycelia. Cycloheximide inhibited the increase in these enzyme activities stimulated by light. When the fis(c) mycelia were exposed to continuous light, an increase in cAMP-binding activity was observed. A possible participation of cAMP in the formation of fruiting bodies in C. macrorhizus is discussed.


31P nuclear magnetic resonance spectroscopy of native and recombined lipoproteins. Native and recombined lipoproteins have been studied by (31)P nuclear magnetic resonance spectroscopy. Very low-, low-, and high-density lipoproteins exhibited characteristic spectra. The main resonances were assigned to phosphatidylcholine and sphingomyelin. Relaxation times for these phospholipids were separately measured in low-density lipoproteins and high-density lipoproteins. The effect of paramagnetic ions (Eu(+++)) on the nuclear magnetic resonance spectrum of high-density lipoproteins is reported.


Prostaglandin F2 alpha concentration in genital tract secretions of dairy bulls.


Prostaglandin E2 and its antagonists: effects on autonomic transmission in the isolated sino-atrial node.


Enzyme polymorphism and metabolism.

Enzyme polymorphism and metabolism.


Prolactin receptors in estrogen receptor-deficient mammary carcinoma. We demonstrate for the first time the presence of specific high-affinity receptors for prolactin in rat mammayy carcinoma. There appear to be no significant differences between normal rat mammary tissue and the transplantable, estrogen receptor-deficient R3230AC tumor with regard to the number of binding sites, the affinity of the receptor for prolactin, or the specificity of binding.


Deoxyribonuclease in the cow snout epidermis.


Mechanism of action of lithium salts. Biochemical aspects.


The role of macrophages in the development of contact sensitivity.


Odland body and intercellular substances.


Functional aspects of dermo-epidermal junction.


The particulate origin of adenylate kinase isozyme 4.


The role of the renal medulla in hypertension.


Virulence of indigenous strains of Entamoeba histolytica in experimental amoebiasis of rats.


Studies on the effect of lowered temperature on Entamoeba histolytica strains, as  a trial to isolate a strain of the "Laredo" type.


Development of globule leucocyte specific granules from lysosomes.


Planning a community mental health program: a case history.


Ultrastructural study of a case of extramammary Paget's disease with special reference to cellular changes in melanocytes and a Langerhans cell.


Special observations in skeletal identification.


The effect of dibutyryl 3'5'-cyclic AMP on the cardiac pacemaker, arrested with reserpine and alpha-methyl-tyrosine.


Medical management of idiopathic facial (Bell's) palsy.


Prostaglandins, cyclic nucleotides and cell function.


Topological analysis of enzymic mechanisms.


Pain and suffering. The gate control theory.


Power spectra of cortical electric activity in the rabbit in relation to conditioned reflexes. In chronic experiments on rabbits spectral and correlation characteristics of cerebral cortical potentials were computed to study the elaboration of a conditioned defense reflex. was found that the frequency spectra of the EEG are subject to consistent changes: a narrow-band frequency constantly developing in the theta range. During exposure to a positive conditioned stimulus, the maximum of this frequency increased relative to the pre-stimulus period and a maximal coherence of electrical processes was observed in a narrow theta range between the electrical processes of the cortical analyzers to which the conditional and unconditional stimuli were addressed. Different relationship was noted in the theta range during presentation of a differential or extinguishing stimuli: it became slightly slower than in the pre-stimulus period, and the coherence of the theta rhythms was less than maximal, remaining high at the other frequencies. It was concluded that the presence of highly coherent electrical processes in the theta range is important for the formation of conditioned reflex in the rabbit cerebral cortex. The processes of internal inhibition are characterized electrographically by disagreement of electrical processes in the theta range in respect to phase or frequency.


The effects of marijuana on human sleep patterns.


Role of the medial zone of the medulla in rhythmic activity of respiratory center neurons.


Urinary tract infection in hospitalized patients.


Cytochrome c reducing substances in photosynthetic electron transport.


Effect of midbrain raphe and lateral mesencephalic stimulation on spontaneous and evoked activity in the lateral geniculate of the cat.


Effects of electrically induced convulsions and transmitter substances on ribonucleic acid turnover in brain slices.


The effect of electrical stimulation, light and amino acids on the efflux of 35S-taurine from the retina of domestic fowl.


Binocular neurons of the rabbit's visual cortex: receptive field characteristics.


Binocular neurons of the rabbit's visual cortex: effects of monocular sensory deprivation.


Studies on the reduction of tetrazolium salts. 3. The products of chemical and enzymic reduction.


Diagnosis of testicular tumours.

Diagnosis of testicular tumours.


Urinary angiotensinogen loss in chronic proteinuric glomerulonephritis.


Resonance Raman spectra of heme proteins. Effects of oxidation and spin state.


The hydrolysis of penicillin G to 6-amino penicillanic acid by entrapped penicillin acylase.


Identification of elastic tissue in human skin viewed in the scanning electron microscope.


Correlations between fatty acid distribution in phospholipids and the temperature dependence of membrane physical state.


Biochemical and genetic studies on mammalian cells.


A comparison of characteristic temperatures for transport in two unsaturated fatty acid auxotrophs of Escherichia coli.


Isolation and characterization of viruses from fetal calf serum.


Electron microscopic observation of the renal lymphatic capillary after injection of ink solution.


Adrenaline, DB-c-AMP and myocardial 45Ca exchange. Comparative studies in rat and guinea-pig auricles.


The effects of 5-hydroxytryptamine and some related compounds on the cat superior cervical ganglion in situ.


Are cholinergic mechanisms involved in morphine effects on motility?


Stimulatory effects of DB-c-AMP and adrenaline on myocardial contraction and 45Ca exchange. Experiments at reduced calcium concentration and low frequencies of stimulation.


Pathogenicity of Mokola, a rabies-related virus, in baby mice.


An electron microscope study of the tegument of the metacercaria and adult of Leucochloridiomorpha constantiae (Trematoda: Brachylaemidae).


Observations on the frog dorsal column nucleus.


Effects of goggle-altered color perception on sleep.


Neurogenic disorders affecting muscle: five-year analysis of muscle biopsies. I. Segmental atrophy.


Community control in mental health: how far can it go?


Brief psychotherapy in the hospital setting: techniques and goals.


Chemotherapy of experimental brain tumors: preliminary studies.


A new viral-hematologic etiology hypothesis for chronic and paroxysmal headache syndromes.


Seasonal change in glycerol kinase activity of goat spermatozoa.


Bronchial carcinoid with choroidal metastasis in an adolescent. Case report and ultrastructural study.


Wilms' tumor--a clinical study of eighty-one patients.


Down's syndrome: establishment of lymphoblastoid cell lines with trisomy 21.


A technique for studying chemotaxis of leucocytes in well-defined chemotactic fields.


Interspecific somatic cell hybridization between asparagine-requiring and drug-resistant cell lines.


Effects of cytochalasin B on cell division and vacuole formation in Tetrahymena pyriformis GL.


Unidirectional loss of human chromosomes in rat-human hybrids.


The deoxyribonucleoside 5'-triphosphate (dATP and dTTP) pool in phytohemagglutinin-stimulated and non-stimulated human lymphocytes.


Comparison of amoebicidal action of antibiotics on Entamoeba histolytica grown axenically & with bacterial or trypanosome associates.


Action potentials in the cochlea. Masking, adaptation and recruitment.


In vitro neoplastic transformation of bovine embryonic urothelium by simian vacuolating virus 40(SV40).


Renal ischemia modified by an angiotensin antagonist.


A comparison of the hormone responsiveness of the prostate and seminal vesicles.


Congenital epulis--a case report.

Congenital epulis--a case report.


A psychophysiological study of nightmares and night terrors. 3. Mental content and recall of stage 4 night terrors.


Massive gingival hyperplasia preceding dental eruption in I-cell disease.


Anomalous flexor sublimis muscle with incipient carpal tunnel syndrome. Case report.


Metabolic differences between normal and neoplastic cells: effects of aminonucleoside on cytoplasmic messenger RNA. Treatment of cultured normal human fibroblasts with an adenosine analog (aminonucleoside of puromycin) rapidly inhibits the appearance of cytoplasmic messenger RNA, identified by its polyadenylate sequence. Similar treatment of SV40-transformed fibroblasts does not lead to such an inhibition. Cordycepin. another analog of adenosine. inlhibits polyadenylate-containing cytoplasmic RNA in both types of cell.


Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus. In mouse cell lines derived from mammary adenocarcinomnas, the synthetic steroid dexamnethasone stimulates production of murine mammary tumor virus. Viral RNA and antigens are increased as much as 20-fold, and culture fluid supernatants from steroid-treated cells contain type B particles with reverse transcriptase. These cells provide a possible tissue culture source of this virus and a model system for studying the mechanism of action of corticosteroids and the regulation of transcription of integrated viral DNA.


Brain tumors presenting with psychiatric symptomatology. (A five-year survey).


Diarrhoea in intensively-reared lambs.

Diarrhoea in intensively-reared lambs.


Ultrastructure of a mycoplasma-like avian conjunctivitis agent.


Properties of mitochondrial thymidine kinases of parental and enzyme-deficient HeLa cells.


Specificity of sugar-phospholipid interactions.


Induction of microsomal aryl hydrocarbon (3,4-benzo(alpha)pyrene) hydroxylase and cytochrome P-450K in rat kidney cortex. I. Characteristics of the hydroxylase system.


Kinetic studies of a hepatoma alkaline phosphatase.


Electron spin resonance of myosin spin labeled at the S1 thiol groups during hydrolysis of adenosine triphosphate.


H2O2 production and cytochrome c peroxidase activity in mitochondria isolated from the trypanosomatid hemoflagellate Crithidia fasciculata.


Effect of interferon on tumor forming ability of Walker carcinosarcoma 256 cells and secretion of exo-toxin by the bacillus, Clostridium welchii (type C).


Subtilopeptidase a cleavage in the cyclic region of the peptide antibiotic polymyxin B1.


Letter: Rapid relaxation of spin equilibrium in ferric myoglobin hydroxide.


The chemistry of penicillanic acids. II. Some reactions of methyl penicillanate and its 6alpha-bromo- and 6,6-dibromo-derivatives.


High-speed liquid chromatographic separation of equine estrogens.


Evaluation of centrifugal chromatography. I. Seperation of steroid hormones on silca gel.


Radiologic seminar. CXXXVI. Linitis plastica: "leather-bottle stomach".


Pancreatic insulin in diabetes mellitus.


Chronic disease of liver and lungs in New Guinea.


Blood-pressure regulation in renal failure.


Steroid biosynthesis by transplantable gonadal tumors of rat.


Receptors for androgens in rat epididymis. Interactions with 5alpha-androstane-3alpha,17beta-diol.


Immunosuppressant agents in the treatment of glomerulonephritis. 1. Corticosteroid drugs.


Effect of adenosine-3',5'-cyclic monophosphate on beta-hydroxy steroid dehydrogenase in the bovine corpus luteum.


Serologic evidence of arbovirus activity in a moose population in Alberta.


Inclusion body hepatitis in a prairie falcon.


Survey for antibody to infectious bovine rhinotracheitis (IBR), bovine virus diarrhea (BVD) and parainfluenza 3 (PI3) in moose sera.


Infectious pancreatic necrosis virus--temperature and age factors in mortality.


The immune response of channel catfish. I. Basic responsiveness to soluble and viral antigens.


Pseudogout syndrome in hospital patients.


Barium enema septicemia. Occurrence in a patient with leukemia.


Photoinactivation of Herpes simplex virus continues to look promising.


On the phospholipids of Culex pipiens fatigans.


Lipid composition and endogenous respiration of pig heart mitochondria.


Postnatal changes in the phospholipid composition of livers from young lambs.


Determination of the position of ethylenic linkages in lipids.


Isomerization of L-glyceraldehyde to dihydroxyacetone during glyceride synthesis by rat liver microsomes.


Inhibition of phospholipase C by isosteric phosphinic acid analogues of lecithin.


Genetic duplications induced at very high frequency by ultraviolet irradiation in Escherichia coli.


Management of spina bifida cystica. II. Initial management of the treated child.


The effects of l,l-dimethyl-4-phenyl-piperazinium (DMPP) in the cat superior cervical ganglion in situ.


Mutants of Salmonella typhimurium deficient in DNA polymerase. I. Detection by their failure to produce colicin E1.


Management of spina bifida cystica. III. Follow-up care and further treatment.


Somatomedin and growth after renal transplantation.


Neuronal circuitry in the nucleus lateralis of the cerebellum.


Quantitative determination of urinary metabolites of cortisol in eluates from thin-layer chromatograms obtained by the partition method.


Hydroxamic acids of natural origin.

Hydroxamic acids of natural origin.


The biosynthesis of pteridines.

The biosynthesis of pteridines.


Clostridium perfringens septicemia complicating degenerating uterine leiomyomas.


Effect of prolonged continuous intracarotid arterial perfusion of hydroxyurea on the normal monkey brain.


The effects of long-term estrogen on hysterectomized women. Presented is a report of 737 women subjected to hysterectomy and subsequently given estrogen therapy with conjugated equine estrogens.  A total of 9899 patient-years were studied.  The original objective of the study was to evaluate the role of estrogen in the development of carcinoma of the breast.  No statistical increase in breast cancer was observed.  The most striking finding was the unexpectedly low incidence of all cancer and the markedly low mortality rate.  A study was carried out on the subjective response of 550 of the women.  Questions were asked on general health, emotional status, and sex.  477 of the women said their health was better, 338 said that their nerves were better, and 181 out of 373 (61 were unmarried) said that sex was better.  It believed that due to the use of estrogen, delays were effected in the onset of osteoporosis, and these resulted in an improvement of the incidence of the skeletal fractures which might be anticipated in this age group.


Studies on control of infections at time of intraocular surgery.


Enzymatic theory of corneal ulceration---a beginning.


Bacterial flora of the lids and conjunctiva before, during, and after intraocular surgery.


Proceedings: How vital is mammography in the diagnosis and management of breast carcinoma?


Effects of REM deprivation and awakening on instrumental performance during stage 2 and REM sleep.


Separation of phosphoribulokinase from enzymes of the Calvin cycle in Hydrogenomonas eutropha H 16.


Hypnotizability predicted by digital computer-analyzed EEG pattern.


Techniques of human breast neoplasm cell culture.


Inhibition and enhancement of mammary tumorigenesis by 7,12-dimethylbenz (a) anthracene in the female sprague-dawley rat.


A chromatographic comparison of the constituents of nutmeg and mace (Myristica fragrans Houtt.) with those of marihuana and hashish (Cannabis sativa L.).


Two-dimensional TLC of lipophilic compounds. Characterization of a mixed stationary phase permitting both adsorption TLC and reversed-partition TLC on one plate.


The carbohydrate moiety of rhodopsin: lectin-binding, chemical modification and fluorescence studies.


Gas-liquid chromatographic separation of equine estrogens.


Evidence for dual corticosteroid-receptor mechanisms in the feedback control of adrenocorticotrophin secretion.


Editorial: Do human tumors show a chromosome pattern specific for each etiologic agent?


Heterogeneity of virus production and antigenicity detected in cell clones derived from a "nonproducer" neoplasm induced by Moloney murine sarcoma virus.


Neoplasms and amyloidosis in strains of mice treated with 3-methylcholanthrene.


Rescue of cell-transforming virus from a non-virus-producing bovine cell culture transformed by feline sarcoma virus.


Mammalian sarcoma-leukemia viruses. I. Infection of feline, bovine, and human cell cultures with Snyder-Theilen feline sarcoma virus.


Immunoglobulin analysis of C57BL mice infected with a radiation-induced leukemia virus.


Replicating type-C virus particles in monolayer cell cultures of tissues from cattle with lymphosarcoma.


Virus particles in primordial germ cells of fetal guinea pigs.


Different chromosome morphology of diploid and aneuploid malignant cells.


In vitro effects of melanocyte-stimulating hormone, adrenocorticotropic hormone, 17 beta-estradiol, or testosterone propionate on Cloudman S91 mouse melanoma cells.


Effect of feline leukemia virus on transferrred hamster fetuses.


Mixed Müllerian tumour of the corpus uteri.


Clinicopathologic conference. Cholecystitis, pericholecystic abscess, and septicemia due to Clostridium perfringens. Case presentation.


Letter: Nephroblastoma.

Letter: Nephroblastoma.


A diallel analysis of the genetic underpinnings of mouse sleep.


Hepatic scintiangiographic patterns.

Hepatic scintiangiographic patterns.


Absence of ponto-geniculo-occipital (PGO) spikes in rats.


Angiographic features of focal lymphoma of the liver.


Comparative study of the incorporation of 32P into the phospholipids of membranes from Novikoff hepatoma ascites cells and liver cells from normal and tumor bearing animals.


The water tank technique: avoidance conditioning as a function of water level and pedestal size.


Diabetes and hypertensive vascular disease. Mechanisms and treatment.


Control of the enzyme systems in plasma renin activity measurement by angiotensin I radioimmunoassay.


Radiopertechnetate flow study and liver scan in a case of benign hepatoma (liver cell adenoma).


Wilm's-aniridia syndrome with transient hypo-gamma-globulinaemia of infancy.


Lymphangiography in patients with pulmonary dysfunction.


The pituitary and primary hypothyroidism. Enlargement and unusual growth hormone secretory responses.


Thyrotoxicosis from molar thyrotropin.

Thyrotoxicosis from molar thyrotropin.


The REM state and drugs.

The REM state and drugs.


The binding of acetylcholine to receptors and its removal from the synaptic cleft. 1. Acetylcholine (ACh) noise and miniature end-plate potentials were recorded with focal external micro-electrodes.2. The effect of prostigmine on the time course of the ;molecular' and ;quantal' transmitter actions was studied. Prostigmine (10(-6) g/ml.) has little or no effect on the duration of the molecular ;gating action', while it greatly prolongs the quantal conductance change.3. After inhibition of ACh hydrolysis, the removal of the transmitter from the synapse is generally too slow to be accounted for by free diffusion. It is suggested that diffusion is delayed by binding to post-synaptic receptors. This is consistent with the finding that receptor blockage by curare or alpha-bungarotoxin shortens as well as reduces quantal transmitter action.4. The correlated effects of the receptor-blocking agents, on size and time course of the miniature end-plate currents, were subjected to a simple analysis. Its result suggests that after inhibition of cholinesterase about two thrids of the quantal packet of ACh combines with post-synaptic receptors.5. During focal external recording the effect of prostigmine on the time course of miniature end-plate potentials can become exaggerated due to what appears to be a compression artifact which obstructs outward diffusion of the transmitter.


Membrane properties and inhibitory innervation of the circular muscle cells of guinea-pig caecum. 1. The membrane properties of the circular muscle cells of guinea-pig caecum and nervous factors influencing the muscle activity were studied with micro-electrodes using partition and field stimulating methods.2. The mean membrane potential was -52 mV. Spontaneous discharges appeared as regular bursts between silent periods, as regular spikes without silent period, or as regular slow potential changes with superimposed spikes.3. Spontaneous spikes with overshoot were frequently observed. The mean maximum rate of rise was 5.2 V/sec. The mean conduction velocity of evoked spikes was 5.4 cm/sec.4. The amplitude of the elctrotonic potential was linearly proportional to the current applied by the partition stimulating method. The spatial decay of the electrotonic potential along the tissue was exponential, with a mean length constant of 1.7 mm.5. The time constants of the membrane calculated from the electrotonic potential, and from the conduction velocity, length constant and time course of the foot of the spike were about 200 and 100 msec respectively. These results indicate that the circular muscle of guinea-pig caecum possesses cable like properties.6. Field stimulation (0.3 msec pulse duration) to the circular muscle evoked three different responses successively, i.e. initial depolarization (initial excitatory junction potential) with or without spike, hyperpolarization (inhibitory junction potential) and delayed depolarization (delayed excitatory junction potential) with or without spikes.7. These three different potential changes were completely blocked by treatment with tetrodotoxin (5 x 10(-6) g/ml.), and both the initial and late excitatory junction potentials were blocked by treatment with atropine (5 x 10(-5) g/ml.).8. The distribution of inhibitory nerves in the circular muscle cells was investigated. The results indicate that inhibitory nerves arise from Auerbach's plexus situated just beneath the taenia coli and the nerve branches spread over the whole distance from one taenia coli to the next along the ciruclar muscle cells, a width of about 3 mm.9. The mean conduction velocity of excitation of the inhibitory nerves was 16.0 cm/sec. Hexamethonium, in a concentration of 5 x 10(-6) g/ml. depolarized the circular muscle membrane and lowered the rate of rise and fall of spike, but did not block the generation of inhibitory junction potentials.


The role of the nurse in a drug crisis center.


The clinical specialist in the geographic unit system.


Corticosteroid therapy for rheumatoid arthritis.


The use of glucocorticoids in intestinal disease.


The radiotherapy of nasopharyngeal angiofibroma.


A computer model for the study of image formation of blood vessels smaller than the exposing focal spot.


One interdisciplinary planning team: a case study.


The use of antibodies to study cell structure and metabolism.


Giant fibro-adenoma (cystosarcoma phyllodes) in adolescent females--a clinicopathological study.


Infantile autism: the puzzle.

Infantile autism: the puzzle.


A follow-up survey of the post-treatment adaptation of former day hospital patients.


Amoebic perforation of the intestine in children.


The pros and cons of methadone maintenance.


The reduction of surgical wound infections by topical cephaloridine: a controlled clinical trial.


Black patient, white therapist.

Black patient, white therapist.


Higher education in nursing.

Higher education in nursing.


Korsakoff's syndrome.

Korsakoff's syndrome.


Cancer of the biliary system: a study of 445 cases.


Multiple intestinal malignancies and prolonged survival in a young woman.


Proceedings: Carcinoma of the urachus.

Proceedings: Carcinoma of the urachus.


Retroperitoneal tumors in children.

Retroperitoneal tumors in children.


Letter: Potential hazards of HClO4 in heated systems where esters are involved.


Letter: Phosphodiesterase: an important role in cyclic nucleotide regulation in the retina.


A depression of cell-mediated immunity to measles antigen in patients with systemic lupus erythematosus. Using the direct migration inhibition test, response to measles antigen in patients with systemic lupus erythematosus (SLE) was found to be decreased when compared with that of normal subjects. No alteration was observed in similar experiments using parainfluenza type 1 and rubella antigens. The specific decrease in measles antigen effect showed no obvious correlation with activity of SLE or with the presence of lymphocytotoxic antibodies. Whether the specificity of the decrease in reactivity is due to some particular relationship between the measles virus or antigen and SLE, or to the possibility that measles reactivity is a more sensitive indicator of a generalized defect of cell-mediated immunity, remains unclear.


Interferon production of human leukemic cells--an in-vitro assay.


Interaction of cholera toxin and toxin derivatives with lymphocytes. I. Binding properties and interference with lectin-induced cellular stimulation. The interaction of cholera toxin and a number of toxin derivatives, containing different proportions of light and heavy toxin-composing subunits (L and H), with mouse lymphocytes was studied. Experiments with [(125)I]toxin showed that a single cell can rapidly, within minutes, bind up to 40,000 molecules of toxin, the association constant was estimated to 7 +/- 4 x 10(8) liters/mol, and binding was found to be very similar at 37 degrees C and 5 degrees C. Immunofluorescence studies revealed that the toxin attachment is located on the cell surface, and that purified L subunit but not H subunit binds to the cells. A natural cholera toxoid, built up by aggregated L subunits, showed almost identical binding properties as toxin to the cells. Pure G(M1) ganglioside, the proposed membrane receptor structure for toxin, prevented entirely the cellular binding of both toxin and toxoid. Cholera toxin in concentrations down to approximately 5 x 10(-11) mol/liter (corresponding to 10 bound molecules/cell) inhibited thymus cells from being stimulated to DNA synthesis by concanavalin A (con A), and spleen cells from such stimulation by phytohemagglutinin. The G(M1) ganglioside but not a series of other pure structurally related gangliosides and neutral glycosphingolipids neutralized this toxin activity. Toxin derivatives which, in similarity with toxin, possessed H as well as L subunits but in other proportions, were potent inhibitors of con A-induced thymocyte stimulation, whereas the natural toxoid (aggregated L subunits), purified toxin L subunit and purified toxin H subunit were up to 300-fold less active on a weight basis. The capacity of cholera proteins to inhibit con A-induced thymocyte stimulation correlated well with their activity in the rabbit intradermal toxicity assay. The inhibitory action of cholera toxin on con A-induced thymocyte stimulation did not depend on decreased cell viability from the toxin treatment, nor was it caused by a reaction between toxin and con A. [(125)I]con A bound equally well to the cells when toxin was present as when it was absent, which proves that the toxin did not compete for cellular con A receptors. Nor did the toxin seem to disturb the general mobility of membrane receptors or their ability to accumulate in caps. It is concluded that the L type of subunit confers rapid and firm binding of cholera toxin to lymphocyte membranes, probably to G(M1) ganglioside receptors. For biologic activity the additional presence of H subunit is important. One manifestation of toxin action on lymphocytes is inhibition of lectin-induced DNA synthesis; probably this effect relates to the ability of cholera toxin to raise the levels of intracellular cyclic 3'5'-adenosine monophosphate.


An apparently new enterovirus isolated from patients with disease of the central nervous system.


Type B hepatitis antigen and antibody among prostitutes and nuns: a study of possible venereal transmission.


Microbiologic study of street heroin and injection paraphernalia.


Virucidal activity of H2O2-generating bacteria: requirement for peroxidase and a halide.


Pulmonary defense mechanisms in consolidated and nonconsolidated regions of lungs infected with Sendai virus.


Subcellular distribution of cardiac glycosides in rabbit and rat myometrium and their binding to isolated plasma membrane.


Chemical studies of potential relevance to penicillin hypersensitivity: kinetic studies of methicillin, phenoxymethylpenicillin and their penicillenic acids.


The ultrastructure and function of the tentacle in Rhyncheta cyclopum Zenker (Ciliatea, Suctorida).


Pharmacological analysis of synaptically mediated increase in cyclic adenosine monophosphate in rabbit superior cervical ganglion.


Mutagenic properties of DNA- and RNA-containing viruses nonvirulent for Drosophilia.


Study of the properties of a DNA-polymerase-deficient mutant of Escherichia coli.


Pharmacological fibrinolysis.

Pharmacological fibrinolysis.


Inhibition of platelet aggregation by isochinoline derivatives.


The mechanism of intragenic recombination.


Paramutation.

Paramutation.


Genetics of resistance to environmental stresses in Drosophila populations.


Pharmacological actions of a novel O-acetylsalicylic acid composition.


Insulin interactions with its receptors: experimental evidence for negative cooperativity.


Cyclic AMP binding to living cells of Dictyostelium discoideum in presence of excess cyclic GMP.


[Prolactin cell tumors in animal experiments and in man].


Determination of oxidation and spin states of heme iron. Resonance Raman spectroscopy of cytochrome c, microperoxidase, and horseradish peroxidase.


Effect of potassium cyanate on the catalytic activities of carbamyl phosphate synthetase.


A new procedure for the reconstitution of biologically active phospholipid vesicles.


Detection of antigens determined by the Epstein Barr virus (EBV) in human lymphoblastoid cell culture lines by elution of specific radioiodine labeled antibody.


Neutrophilic leukocytes in immunologic reactions in vitro. 3. Pharmacologic modulation of lysosomal constituent release.


Mammalian collagenases.

Mammalian collagenases.


Lipid lowering drugs and hyperlipidaemia.


The sequential inactivation of glycogen phosphorylase and activation of glycogen synthetase in liver after the administration of glucose to mice and rats. The mechanism of the hepatic threshold to glucose.


Pulse-recovery studies on cycloheximide-insensitive protein synthesis in Neurospora. Association of products with cytochrome-oxidase.


A comparison of mitochondrially synthesized proteins from whole mitochondria and cytochrome oxidase in Neurospora.


Measurements of the concentration of active sites in preparations of yeast alcohol dehydrogenase.


A simplified procedure for the assay of adenosine 3':5'-monophosphate by the activation of liver phosphorylase.


The effect of adenosine analogues on the ATP-pyrophosphate exchange reaction catalysed by methionyl-tRNA synthetase.


Study of some glycolytic enzymes of mouse fibrosarcoma.


Collagenolytic activity of dental plaque associated with periodontal pathology. Certain dental plaques, removed from sites of gingival and periodontal pathology in mentally retarded, institutionalized individuals, when incubated in phosphate buffer with Achilles tendon collagen, gave rise to an increase in ninhydrin-positive material. These plaques, while showing great variability, released significantly more ninhydrin-positive material per milligram of plaque (wet weight) than did either the endogenous or heat-treated controls. Certain plaques could also break down soluble, tritiated, labeled collagen isolated from the calvaria of chicken embryos. Bacteroides melaninogenicus and Clostridia histolyticum were found in plaques by either fluorescent antibody or cultural methods. C. histolyticum, when detected, accounted for about 0.01 to 0.1% of the bacteria in plaque. A conspicuous isolate from some plaques was a Bacillus species which rapidly liquefied gelatin. Cell-free supernatants of this organism were able to degrade about 50 to 70% of the soluble collagen when incubated at 36 C. C. histolyticum ATCC 8034 caused an 80% degradation of the collagen under the same conditions of incubation. The Bacillus strains were facultative, could ferment glucose, reduced nitrate to nitrite, and were catalase, indole, and urease negative. The limited taxonomic information for the isolates is compatible with the description given for Bacillus cereus.


Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils. Human polymorphonuclear neutrophils (PMN) were made anaerobic by nitrogen washout (oxygen saturation <1%, Eh < -42 mV at pH 7.0), and the ability of the cells to kill bacteria was assayed and compared to the bactericidal activity of aerobic PMN. Anaerobic PMN were able to kill Staphylococcus epidermidis, Enterococcus, viridans streptococci, Pseudomonas aeruginosa, Peptostreptococcus anaerobius, Bacteroides fragilis, Clostridium perfringens, and Peptococcus magnus normally. Organisms that were not killed normally by anaerobic PMN included Staphylococcus aureus (strains Wood 46 and 502a), Escherichia coli, Serratia marcescens, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella typhimurium. These findings suggest that mechanisms other than those dependent on hydrogen peroxide may be important in the killing of some bacteria.


Replication or inactivation of different viruses by human lymphocyte preparations. Cultures of phytohemagglutinin-stimulated, unstimulated, and frozen and thawed mononuclear (as controls) cells from normal human donors were infected with different viruses. A variable pattern of virus infectivity was noted, and influenza and Coxsackie virus were rapidly inactivated even by stimulated lymphocytes. Direct inactivation by lymphocytes may be one form of host defense in infection by some viruses.


Capsular polysaccharide of Clostridium perfringens Hobbs 10. A capsular polysaccharide was isolated from a strain of Clostridium perfringens Hobbs 10 type A by cold-water extraction of whole, heavily encapsulated cells. The water-soluble polymer was isolated by alcohol precipitation and purified by treatment with chloroform-butanol, cetytrimethylammonium bromide, and column gel permeation chromatography by using Bio-Gel A-5m agarose. The formation of a single precipitin line, when the isolated polysaccharide was reacted with its homologous antisera by double diffusion in gel, was considered a criterion of immunochemical purity. The purified polymer appeared as a single peak when eluted from diethylaminoethyl-Sephadex with a linear gradient of NaCl. The polysaccharide was composed of glucose, galactose, galactosamine, and iduronic acid in a molar ratio of 4.1:5.1.7:1, respectively. These constituents accounted for 83% of the dry weight. The polysaccharide appeared to have a molecular weight of 40,000 and exhibited aggregation up to 120,000. A trace of peptide material could not be removed during purification.


Epidemiological and biochemical studies on thiamine-less dwarf-colony variants of Staphylococcus aureus as etiological agents of bovine mastitis. Dwarf-colony (D) variants of Staphylococcus aureus are relatively widespread etiological agents of bovine mastitis in Israel. D strains grow on ordinary solid nutrient media with pinpoint, transparent colonies. An epidemiological study indicated that these variants are more communicable than normal (N) S. aureus strains. Biochemically, several types of metabolic defects have been demonstrated among the D strains: strains from one herd were pantothenate-less, most of the other isolates were thiamine-less, and a few of them were both thiamine-and pantothenate-less. Among the thiamineauxotrophs were a few (from one herd) which were unable to concentrate thiamine-thiazole, whereas all of the other strains required thiamine-pyrimidine (HMP) in the form of pyrophosphate (HMP-PP). At least some of these D strains were defective in three functions: concentrative uptake of HMP, phosphorylation of HMP, and phosphorylation of HMP-monophosphate. Since mutants with normal growth occurred readily, we assumed that the information for these three functions exists on a polycistronic operon. Quantitative requirements for HMP-PP varied highly among the D strains, and other data also indicated that HMP-PP-requiring strains had not developed from a common source.


Lipid catabolism of relapsing fever borreliae. Relapsing fever borreliae require lipid compounds for growth in vitro. In this study, the major pathways of lipid catabolism in three species of tick-borne relapsing fever borreliae were investigated. Thin-layer chromatography was used to compare chloroform-methanol extracts of fresh culture media with extracts of exhausted culture media after organisms were removed by centrifugation. The chromatographic data demonstrated that lysolecithin was removed from the culture media during growth of the spirochetes, whereas lecithin, sphingomyelin, triglycerides, and cholesterol esters were not affected by growth of the organisms. Sonic extracts of the organism were tested for the presence of specific enzymes of lipid catabolism. Lysolecithinase, glycerophosphorylcholine diesterase, and acid phosphatase activities were demonstrated. Thus, these organisms can sequentially dissimilate lysolecithin to fatty acids, choline, inorganic phosphate, and glycerol. Assays for phospholipases A, C, and D, alpha-glycerophosphate dehydrogenase, alkaline phosphatase, and lipase were negative.


Combined effects of phytohemagglutinin and staphylococcal enterotoxin B on deoxyribonucleic acid synthesis during blast transformation in human lymphocytes. Three mitogenic agents, phytohemagglutinin (PHA), staphylococcal enterotoxin B (SEB), and concanavalin A (Con A) were tested for their effects on deoxyribonucleic acid (DNA) synthesis in the normal human lymphocyte. When optimal concentrations of PHA and SEB were combined, tritiated thymidine incorporation in lymphocytes derived from several donors was enhanced significantly. In the presence of graded concentrations of one of these mitogens added to fixed optimal concentrations of the other, this enhancement was shown to be additive. By contrast, when PHA or SEB were combined with Con A, the resulting thymidine incorporation was slightly lower than for either mitogen alone. An inhibition of further thymidine incorporation when puromycin was added to lymphocytes incubated with PHA and SEB suggested that the additive effect of these mitogens was due to increased enzyme synthesis. To define potential differences in mechanisms of action underlying the additive effect of SEB and PHA, the relative contribution of the de novo and salvage pathways for pyrimidine biosynthesis was tested with cytidine, a specific salvage pathway inhibitor. Cytidine (10(-3) M) inhibited synthesis through the salvage pathway, but did not significantly alter induction of carbamyl phosphate synthetase II, the rate-limiting enzyme for the de novo pathway. An inhibition of DNA synthesis by millimolar cytidine concentrations in lymphocytes incubated with PHA or SEB, singly or in combination, suggested that pyrimidines for the observed enhancement of DNA synthesis were derived largely via the salvage pathway.


Antibody against Clostridium perfringens type A enterotoxin in human sera. Antibody against Clostridium perfringens type A enterotoxin was found in 82% of Brazilian and 65% of American serum samples.


Stereoelectronic properties of metalloenzymes. II. Effects of ligand coordination on the electron spin resonance spectrum of galactose oxidase as a probe of structure and function.


Stereoelectronic properties of metalloenzymes. I. A comparison of the coordination of copper (II) in galactose oxidase and a model system, N,N'-ethylenebis(trifluoroacetylacetoniminato)copper(II).


Reaction of phenylmethanesulfonyl-alpha-chymotrypsin with hydrogen peroxide and other nucleophiles.


Cation radicals of tetraalkylporphyrins.


Studies on the sarcoplasmic reticulum of rat, cat and sheep intrafusal fibres.


The ultrastructure of Leydig cells in the testis of the domestic fowl.


Pressure accelerated chromatography through microparticulate silica columns packed by centrifugation.


Report on microbiological methods.

Report on microbiological methods.


Tumorous hyperparathyroidism in hypophosphatemic vitamin D--resistant rickets.


Novel analogs of nucleoside 3',5'-cyclic phosphates. I. 5'-Mono- and dimethyl analogs of adenosine 3',5'-cyclic phosphate.


Phospholipids in biology and medicine (second of two parts).


Eighteenth Bowditch lecture. Motor aspects of cerebellar control.


Surgery of atherosclerosis below the inguinal ligament. The value of profundaplasty.


Role of viruses in the etiology of congenital malformations.


Separation of prostaglandins A, B and C by thin-layer chromatography on ferric chloride impregnated silica gel. A convenient thin-layer chromatographic method for separating and isolating the isomeric prostaglandins A1, B1, and C1 under non-alkaline conditions was developed as a prerequisite for the in vivo investigation of the metabolism of PGA1.  The method was based on thin-layer chromatography on ferric chloride impregnanted silica gel.  The resolution of prostaglandin A from prostaglandin B proved to be superior to that on silica-gel thin-layer chromatography plates impregnated with silver nitrate.  Thin-layer chromatography has the advantages of simplicity, speed, and amenability to radioactivity scanning.  The method should find application in the separation of other prostaglandins, as well as a number of other classes of compounds.


A review of myelography.

A review of myelography.


Food safety and food poisoning.

Food safety and food poisoning.


On the effects and the mechanism of action of corticosteroids in normal and neoplastic target tissues: findings and hypotheses, with a review of information on intracellular steroid receptors in general.


The metabolism of tamoxifen (I.C.I. 46,474). I. In laboratory animals.


Effect of cyclic AMP and polyadenylic acid treatment on the delayed hypersensitivity of guinea pigs.


Antiviral effect of 1-aminoadamantane derivatives in vitro.


The National Center for Health Statistics. A research resource for pediatricians.


Use of oxandrolone for growth stimulation in children.


A new look at the brain-damaged adult.

A new look at the brain-damaged adult.


Herpesvirus hominis infections in the fetus and the newborn.


The process of collaboration. II. Implementation and its discontents or you don't learn that in school.


Incorporation and replication of foreign metaphase chromosomes in cultured mammalian cells.


Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.


c-AMP and the cell cycle: inhibition of growth stimulation.


The federal role in health: a conversation with Charles C. Edwards, M.D., assistant Secretary for Health, Department of Health, Education, and Welfare.


Effect of induced interferon in experimental rhinovirus infections in volunteers. Nasal administration of an interferon inducer, CP-20,961 (N, N-dioctadecyl-N', N'-bis(2-hydroxyethyl)propanediamine), was evaluated in a double-blind, placebo-controlled study of experimental rhinovirus infection in 29 volunteers. Detectable nasal interferon developed in 10 of 15 subjects treated with CP-20,961, and 2 of 14 in the group receiving placebo (P < 0.006). Titers of CP-20,961-induced interferon ranged from 10 to 250 international units, concentrations similar to those observed in rhinovirus infections. Ten in the CP-20,961 group and eight in the placebo group became ill (P > 0.05). However, mean total sign scores were significantly diminished among CP-20,961-treated subjects as compared with placebo-treated subjects (P < 0.025). No significant effect was noted on quantitative virus shedding patterns or neutralizing antibody responses. These findings suggest that such concentrations of induced interferon do not protect against rhinovirus infection, and that factors other than interferon may be important in recovery from rhinovirus infection.


Prevention of malnutrition.

Prevention of malnutrition.


Prospects for decline in population growth in the developing nations.


Attenuation of rhinovirus type 15: relation of illness to plaque size. Rhinovirus type 15 passaged three times in WI-38 cells produced illness in only one of 35 volunteers, but infection resulted in formation of significant quantities of antibodies in the serums of 100%, and in nasal secretions of 89%, of infected volunteers. The inoculum contained large- (60%) and small- (40%) plaque variants. "Purified" large- and small-plaque inocula were prepared, and each was administered to 20 volunteers. There was a significant association of illness with the small-plaque inocula (P < 0.01) but the incidence of infection, quantitative virus shedding patterns, and mean serum and nasal secretory antibodies were not significantly different between the two groups. These findings suggest that plaque size may be an in vitro marker of attenuation of illness production by rhinoviruses.


The excitable membrane-biophysical theory and experiment.


Acceptance of Elizabeth Blackwell Award.


A new era: challenges for the woman physician. (Hey, no fair. There are hardly any lady doctors.).


Fluorescence studies of possible protein-protein interactions in model lipoprotein complexes.


Cell agglutination mediated by concanavalin A and the dynamic state of the cell surface.


Initiation of DNA replication in mammalian cells and its inhibition by reovirus infection.


Early components of the averaged electroencephalic response to constant level clicks during all-night sleep.


The enzymic make-up of the left and right ventricles of the normal rat heart and the changes shown on exposure to exercise and hypoxic exercise.


Consequences of excess hormonal secretion in digestive disease.


The role of phosphorylation in the uptake of thymidine in mammalian cells.


Separation and radioimmunoassay of f alpha prostaglandins using silica gel micro columns.


Stimulatory effects of prostaglandin e-1 on rat anterior pituitary cyclic AMP and luteinizing hormone release.


Induction of liver tumours in rats by a single treatment with nitroso compounds given after partial hepatectomy.


TRH and prostaglandin action on rat anterior pituitary: dissociation between cyclic AMP levels and TSH release.


Prostaglandins and cyclic nucleotides in Moloney sarcoma tumors.


Granular cell myoblastoma of the esophagus.


Propagation of some common viruses in human embryonic brain cells.


Clear-cell carcinoma of the liver. A clinicopathologic study of 13 patients.


Acquired cytomegalovirus infection presenting as viral endometritis.


Proceedings: Carcinoma of the soft palate and uvula.


Non-chromaffin paragangliomatosis manifesting as a cold thyroid nodule.


Proceedings: Serum alpha-fetoprotein and Ga67 citrate uptake in hepatoma.


Proceedings: Arteriography in insulinoma.


Multiple primary bronchogenic carcinoma with a review of the literature.


Ozone effect on respiratory deposition of vesicular stomatitis virus aerosols.


Beclomethasone in childhood asthma.

Beclomethasone in childhood asthma.


Pulmonary infections complicating treatment of multiple sclerosis. Occurrence with administration of corticotropin or adrenal steroids.


Reye syndrome. An association with type I vaccine-like poliovirus.


Parainfluenza, histocompatibility, and multiple sclerosis. Association of parainfluenza antibodies and histocompatibility types in MS and optic neuritis.


Genetic heterogeneity of hereditary sensory neuropathy.


MU-chain disease in an African patient.

MU-chain disease in an African patient.


Hemoglobin Duarte: (alpha2beta2 62(E6)Ala leads to Pro): a new unstable hemoglobin with increased oxygen affinity.


Importance of central catecholaminergic neurones in the development and maintenance of renal hypertension.


Metabolic clearance rate of angiotensin II in arterial hypertension.


Spironolactone and amiloride in the treatment of low renin hyperaldosteronism and related syndromes.


Synthesis of renin inhibitors.

Synthesis of renin inhibitors.


Effects of angiotensin II on adenylcyclase activity, phosphodiesterase activity and on the level of cyclic AMP in rat uterus.


Angiotensin-salt hypertension.

Angiotensin-salt hypertension.


Plasma renin activity and plasma renin substrate in hypertension associated with steroid excess.


Experimental studies on the mechanism of adrenocorticotrophic hormone-induced hypertension in the sheep.


Oral contraceptives and hypertension.

Oral contraceptives and hypertension.


Renin release and renal vasomotor changes during stimulation of the brain stem in the cat.


Diseases and surgery of the nose.

Diseases and surgery of the nose.


Hormonal profiling in the diagnosis and treatment of essential hypertension.


Effects of diphenylhydantoin upon glucose-induced insulin secretion in three patients with insulinoma.


Insulin receptors in human and animal placental tissue.


Cytologic and functional changes in the adrenal cell culture from a human fetus under the action of o, p1-DDD. I. Morphological study.


Cytologic and functional changes in the adrenal cell culture from a human fetus under the action of o, p1-DDD. II. Study of corticosteroid production.


Affinity chromatography on immobilised adenosine 5'-monophosphate. 3. The binding of glycerokinase and lactate dehydrogenase in relation to column geometry and dynamics.


Comparative studies on the template-initiator requirements of the chick-embryo DNA polymerase I and the avian-myeloblastosis-virus DNA polymerase.


Affinity chromatography on immobilised adenosine 5'-monophosphate. 4. Variation of the binding of dehydrogenases and kinases with pH.


Affinity chromatography on immobilised adenosine 5'-monophosphate. 5. Some applications of the influence of temperature on the binding of dehydrogenases and kinases.


Animal DNA-dependent RNA polymerases. Analysis of the RNAs synthesized on Simian virus 40 superhelical DNA by mammalian RNA polymerases AI and B.


Animal DNA-dependent RNA polymerases. Studies on the reaction parameters of transcription in vitro of Simian virus 40 DNA by mammalian RNA polymerases AI and B.


Inhibition of spontaneous aggregation by wheat germ agglutinin in suspensions of BALB-C-3T3 and BHK21 tissue culture fibroblasts.


Animal DNA-dependent RNA polymerases. Studies on the binding of mammalian RNA polymerases AI and B to Simian virus 40 DNA.


Cyclic AMP dependent regulation of mitosis in human lymphoid cells.


Synthesis of DNA-binding protein in WI-38 cells stimulated to synthesize DNA by medium replacement.


Thymidine transport by Novikoff rat hepatoma cells synchronized by double hydroxyurea treatment.


Myogenesis in vitro. Enhancement by dibutyryl cAMP.


Tes and HEPES buffers in mammalian cell cultures and viral studies: problem of carbon dioxide requirement.


Chromatin fractionation of normal and virus-transformed cells in culture.


Pneumoconiosis.

Pneumoconiosis.


Chemical labeling and freeze-fracture studies on the localization of rhodopsin in the rod outer segment disk membrane.


Prolonged administration of Penicillium viridicatum to mice: preliminary report of carcinogenicity.


Corticosteroid therapy in corneal disease. I.


Current therapy of herpes simplex virus infection of the cornea.


Investigations on serum lipid components and serum vitamin E in iron deficiency anemia.


Treatment of corneal ulcers with collagenase inhibitors.


In vitro phosphate transport in chick ileum: effect of cholecalciferol, calcium, sodium and metabolic inhibitors.


Fluctuation of the nucleotide pools of flavinogenic and nonflavinogenic strains of Eremothecium ashbyii grown in the presence of purines.


Cyclic adenosine 3':5'-monophosphate and protein kinase levels in developing bovine spermatozoa.


Letter: Estrogen binding in breast cancer.


Prevention of gestational trophoblastic disease by use of actinomycin D in molar pregnancies.


Inclusion-body beta-thalassemia trait. A form of beta thalassemia producing clinical manifestations in simple heterozygotes.


Arrhenoblastomas and associated ovarian pathology.


A new oral amoebicid (RO 7-0207) in the treatment of intestinal amoebiasis.


Arrhenoblastoma associated with adrenal androgenic hyperfunction.


Chemistry and biologic activity of the complement system.


Synthesis and metabolism of complement proteins.


Editorial: Implications of equine virus abortion.


Morphologic revertants of murine sarcoma virus transformed nonproducer BALB-3T3: selective techniques for isolation and biologic properties in vitro and in vivo.


Cell killing by viruses. I. Comparison of cell-killing, plaque-forming, and defective-interfering particles of vesicular stomatitis virus.


Conditional lethal mutants of vesicular stomatitis virus. I. Phenotypic characterization of single and double mutants exhibiting host restriction and temperature sensitivity.


Conditional lethal mutants of vesicular stomatitis virus. II. Synthesis of virus-specific polypeptides in nonpermissive cells infected with "RNA-" host-restricted mutants.


The molecular size of the herpes simplex virus type 1 genome.


Interferon production by individual cells.


Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.


An improved murine leukemia virus immunofluorescence assay.


Structure of the Mengo virion. I. Polypeptide and ribonucleate components of the virus particle.


In vitro association of vesicular stomatitis virus proteins with purified HeLa and erythrocyte plasma membranes.


Isolation, complementation and preliminary phenotypic characterization of temperature-sensitive mutants of herpes simplex virus type 2.


Antigens specified by herpesviruses. 3. Viral-induced nuclear polypeptides.


Therapy of herpes simplex with heat inactivated Herpes virus hominis type 1 and type 2.


Effect on steroid metabolism of STC-407 and cyproterone acetate in female hirsutism.


Plasma free fatty acid and acute vascular injury.


Cholangiocarcinoma with hypercalcemia--a case report studied by electron-microscope and radioimmunoassay.


Determination of the amount of bone in the metacarpal.


Should we always keep controlling?

Should we always keep controlling?


Diet and bone rarefaction in old age.

Diet and bone rarefaction in old age.


Editorial: Viruses and arteriosclerosis.


Finite level models of biological systems.


Glioblastoma of the optic chiasm.

Glioblastoma of the optic chiasm.


The radiological importance of soft-tissue lesions related to the skeleton.


Cullen's sign, a feature in liver disease.