Ultrastructure of a human chorionic gonadotropin-and adrenocorticotropin-responsive functioning Sertoli-Leydig cell tumor (type I).


Fucosylglycolipid metabolism in oncornavirus-transformed cell lines.


Oestrogenic stimulation of macromolecular synthesis is correlated with nuclear hormone-receptor complex in neonatal rat uterus.


Variations among sublines of inbred AKR mice.


Secretion of growth hormone in the rat.

Secretion of growth hormone in the rat.


The intestinal flora of pigs with special reference to Clostridium perfringens.


Prostaglandin receptors in the hamster uterus during the estrous cycle.


Correlation between measures of the rapid eye movements of wakefulness and sleep.


REM sleep interruption: experimental shortening of REM period duration.


Brain tumor types induced by the Schmidt-Ruppin strain of Rous sarcoma virus in inbred Fischer rats.


Light and electron microscopic studies of mouse CNS after subcutaneous administration of the E and M variants of the encephalomyocarditis virus.


The effects of methoxyflurane on sympathetic ganglionic transmission.


Tear flow in man.

Tear flow in man.


Phase transitions in lipids.

Phase transitions in lipids.


Motion of spin labeled fatty acids in membrane structures.


Interrelationship between the kinds of dietary carbohydrate and fat in hyperlipoproteinemic patients. 1. Sucrose and starch with polyunsaturated fat.


Membrane lipid pattern and non-electrolytes permeability in Salmo trutta L. red blood cells.


Inhibitory effect of chick interferon and interferon inducer (rI:rC) on the induction of cellular DNA synthesis and transformation of chick embryo fibroblasts infected in culture with Rous sarcoma virus.


Sperm velocity and morphology in 1727 ejaculates with normal sperm count.


The prevention of postoperative pelvic adhesions following conservative operative treatment for human infertility. A final 3-year follow-up report. A combination of Decadreon (dexamethasone) and Phenergan (promethazine) was tested in this collaborative study of 240 infertility patients as a means of preventing postoperative adhesion formation after simple pelvic surgery.  24 patients were lost to follow-up.  The overall pregnancy rate among the 240 patients was 51.7% (124): 49% with primary infertility and 58% with secondary infertility conceived after surgery.  90 full-term deliveries were recorded, 27 spontaneous abortions occurred, and 7 ectopic gestations were conceived.  In all, 31 cases were reinspected for various reasons after the drug treatment postsurgery;  42% showed no adhesions, 23% showed minimal adhesions, and 35% had significant adhesions.  Complications coincident with use of the combined medication were seen in 2.2% (11 of 240) patients.  Though this study lacked controls, the authors feel thta the use of corticosteroids postoperatively after pelvic intervention may control the numbers of adhesions which form postoperatively.


Effect of dietary starches on the serum, aorta and hepatic lipid levels in cholesterol-fed rats.


Plasma LH-HCG, 17 -estradiol, estrone and progesterone monitoring around ovulation and subsequent nidation. At the University Hospital in Louvain, Belgium, plasma hormonal fluctuations in 6 women, aged 16-46, were studied.  Measurements were made during the nonpregnant state and in the 4 women who conceived also in the period around nidation.  Measurements during the nonpregnant state yielded expected results: Preovulatory midcycle luteinizing hormone (LH) surge preceded by increase of estrone and estradiol, sudden fall of estrogens and rise of progesterone during or just after LH  surge, and secondary estrogen rise together with progesterone peak during the luteal phase accurred.  In the women implantation occurred, human chorionic gonadotropin (HCG) started to rise sharply 10 or 11 days after the LH surge.  Progesterone and estrogen levels remained high during the implantation period.  In the patient who aborted at 17 weeks of pregnancy, the HCG rise was slower and the secondary rise of estradiol was rather limited; progesterone levels, however, appeared to be normal.  An estradiol surge may occur just prior to implantation.


Interrelationship between the kinds of dietary carbohydrate and fat in hyperlipoproteinemic patients. 3. Synergistic effect of sucrose and animal fat on serum lipids.


Penetration of spermatozoa into the uterine mucosa: a possible cause of infertility in the human.


Clomifen in the treatment of anovulatory women.


Interrelationship between the kinds of dietary carbohydrate and fat in hyperlipoproteinemic patients. 2. Sucrose and starch with mixed saturated and polyunsaturated fats.


The effect of synthetic luteinizing hormone-releasing hormone on blood levels of luteinizing hormone and follicle stimulating hormone in Klinefelter patients.


Sonographic visualization of coexisting gestation sac and I.U.D. in the uterus and a consideration of a causative factor of accidental pregnancy.


Reactions between nitroxyls and radiation-induced long-lived DNA-transients.


Effect of a hypolipidaemic drug (CH 13,437) on plasma and tissue lipids, and on the intravenous fat tolerance in man.


H-adduct radicals in x-irradiated frozen solutions of uracil derivatives.


Plasma phospholipids in type IV hyperlipoproteinemia.


Radical yield in x-irradiated biological molecules. I. Glycine.


The pathogenesis of corneal inflammation due to Herpes simplex virus. I. Corneal hypersensitivity in the rabbit.


Role of cyclic AMP in mitogen induced transformation of human peripheral leukocytes.


Neutralizing antibodies to Epstein-Barr virus in healthy populations and patients with infectious mononucleosis.


Herpesvirus hominis infection in newborn mice. I. An experimental model and therapy with iododeoxyuridine.


Experimental animal model for encephalitis due to herpes simplex virus.


Isolation of herpesviruses from trigeminal ganglia of man, monkeys, and cats.


Viruses causing common respiratory infections in man. II. Enteroviruses and paramyxoviruses.


The role of water in the origin of life and its function in the primitive gene.


Relation between the reaction cytochrome oxidase-oxygen and oxygen uptake in cells in vivo. The role of diffusion.


Virus specific surface antigen in cells productively infected with polyoma virus.


Infection with herpes-simplex viruses 1 and 2. 3.


Intracranial tumors of apoplectiform onset.


Resistance of rabbit corneal epithelium to herpes simplex virus.


Bacterial infections in immunosuppressed patients: diagnosis and management.


Immunosuppression and virus infections.

Immunosuppression and virus infections.


Histocompatibility antigens of human plasma. Localization to the HLD-3 lipoprotein fraction.


Transduction by bacteriophage MU-1.

Transduction by bacteriophage MU-1.


Polyoma gene function required for viral DNA synthesis.


Testing by the "paired-label" antibody binding technique for feline leukemia virus-induced cell surface antigens (FeLV-CSA) on the surface of human rhabdomyosarcoma cells releasing RD-114 virus.


Murine mammary tumor cell clones with varying degrees of virus expression.


Effect of 2-deoxy-D-glucose on cell fusion induced by Newcastle disease and herpes simplex viruses.


Structural components of Sendai virus. Serological and physicochemical characterization of hemagglutinin subunit associated with neuraminidase activity.


Studies on herpes simplex virus DNA: denaturation properties.


Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus.


Synthesis of Epstein-Barr virus antigens and DNA in activated Burkitt somatic cell hybrids.


Fine structural alterations with age in the fat body of the adult male housefly, Musca domestica.


Quantitative microscopical studies of the mouse peritoneal macrophage following stimulation in vivo.


The development of synapses in vitro between previously dissociated chick spinal cord neurons.


Ultrastructural localization of glycogen in erythrocytes and developing erythrocytic cells in normal human bone marrow.


Fine structure of the adenohypophysis in immature sockeye salmon, Oncorhynchus nerka.


Ultrastructure of the pseudohermaphrodite rat testis.


Observations on skin structure and sloughing in the stone fish Synanceja verrucosa and related fish species as a functional adaptation to their mode of life.


An analysis of some aspects of synaptosomal ultrastructure by the use of serial sections.


Small-sized haemagglutinin of vesicular stomatitis virus released spontaneously and with Nonidet P 40.


Typing of Herpesvirus hominis strains by indirect immunofluorescence and biological markers.


Granular cell myoblastoma of multicentric origin involving the vulva: a case report.


Spin changes in hemoproteins.

Spin changes in hemoproteins.


The Chediak-Higashi syndrome. Fine structure of giant inclusions in freeze-fractured neutrophils. The technic of freeze-fracture and etching has been used in the present study to examine the fine structure of giant inclusions in circulating leukocytes from a patient with the Chediak-Higashi syndrome (CHS). The surface granularity of the membranes enclosing the giant inclusions differed slightly from that of normal sized organelles. Two types of giant granules were distinguished in replicas of freeze-fractured CHS neutrophils. The difference in fine structure suggests that one variety is a massively enlarged, but essentially unaltered primary lysosome, while the other develops as a result of continued fusion of small organelles with huge inclusions throughout the stages of polymorphonuclear leukocyte maturation.


Evolving behavior in the clinical and experimental amphetamine (model) psychosis.


The dream-protein hypothesis.

The dream-protein hypothesis.


Alterations of liver metabolism associated with experimental acute pancreatitis.


Diarrheogenic tumor of the pancreas.

Diarrheogenic tumor of the pancreas.


Nonbacterial pneumonitis with antineoplastic therapy.


Enuresis.

Enuresis.


Active, water-insoluble derivatives of D-glucose oxidase and alginic acid, chitin, and Celite.


Etiology of breast cancer. II. Epidemiologic aspects. The epidemiology of breast cancer is reviewed with particular emphasis on its etiology. A number of studies suggest that differences in breast cancer incidence are associated with differences in marital status, number of pregnancies, age at menarche, age at menopause, height and weight, socioeconomic status, geographic location and residence. However, in no case is the evidence adequate to establish a "cause and effect" relationship. The genetic component of these associations may be of primary importance, while other conditions such as marital status are probably indirect reflections of the operation of more fundamental factors.There is a general consensus that endocrine factors play an important part in mammary cancer occurrence. At present, the association between breast cancer and the presence of the virus-like (type B) particles in human milk is not established.


Measurement of urinary aldosterone by a simplified radioimmunoassay procedure.


Automated primary screening for hyperlipoproteinemias.


Action of imipramine on a serotonergic synapse.


The effects of hemicholinium-3 in an insect neuromuscular preparation.


Acetylcholine receptors and the diphasic action of nicotine in the American cockroach, Periplaneta americana (L.).


Glycogenolysis and lipolysis in Gallus domesticus during the perinatal period.


Pituitary-adrenal axis activation as a function of hypothalamic 5-hydroxytryptamine titre.


Neuromuscular block in insects caused by the venom of the digger wasp Philanthus triangulum F.


Transmitter substances in insects: a review.


High resolution deuterium magnetic resonance: an approach to the study of molecular organization in biological membranes and model systems.


Anaerobic cilio-excitation and metabolic stimulation by 5-hydroxytryptamine in bivalve gill.


Activation by glucose of adenyl cyclase in pancreatic islets of the rat.


Intracranial tumors in India: incidence and variations.


Incidence and behavior pattern of intracranial tumors in Ceylon.


Nerve sheath tumors.

Nerve sheath tumors.


Demonstration of iodide transport defect but normal iodide organification in nonfunctioning nodules of human thyroid glands. Benign and malignant nodules in human thyroid glands, which did not concentrate iodide in vivo, were also unable to accumulate iodide in vitro. The mean thyroid-to-medium ratio (T/M) in seven benign nodules was 0.8+/-0.2 compared with 7+/-2 in adjacent normal thyroid tissue. In four malignant thyroid nodules, the mean T/M was 0.5+/-0.1 compared with 11+/-4 in adjacent normal thyroid. Despite the inability of such nodules to concentrate iodide, iodide organification was present but was only one-half to one-third as active as in surrounding normal thyroid. Thyroid-stimulating hormone (TSH) increased iodide organification equally in both benign nodules and normal thyroid although it had no effect in three of the four malignant lesions. The reduction in organification is probably related to the absence of iodide transport, since incubation of normal thyroid slices with perchlorate caused similar diminution in iodide incorporation but no change in the response to TSH. Monoiodotyrosine (MIT) and di-iodotyrosine (DIT) accounted for most of the organic iodide in both the nodules and normal tissue. The MIT/DIT ratio was similar in normal and nodule tissue. The normal tissue contained much more inorganic iodide than the nodules, consistent with the absence of the iodide trap in the latter tissue. The thyroxine content of normal thyroid was 149+/-17 mug/g wet wt and 18+/-4 mug/g wet wt in the nodules. The transport defect in the nodules was not associated with any reduction in total, Na(+)-K(+)- or Mg(++)-activated ATPase activities or the concentration of ATP. Basal adenylate cyclase was higher in nodules than normal tissue. Although there was no difference between benign and malignant nodules, the response of adenylate cyclase to TSH was greater in the benign lesions. These studies demonstrate that nonfunctioning thyroid nodules, both benign and malignant, have a specific defect in iodide transport that accounts for their failure to accumulate radioactive iodide in vivo. In benign nodules, iodide organification was increased by TSH while no such effect was found in three of four malignant lesions, suggesting additional biochemical defects in thyroid carcinomas.


AY-9944 inhibition of sterol biosynthesis in Chlorella emersonii.


Search for prostaglandin A1 in onion.

Search for prostaglandin A1 in onion. Thus far there have been no reports that prostaglandins have been isolated from plant materials.  Numerous plant materials were screened using this layer chromatographic technique to determine if such materials could contain prostaglandins.  Following this initial screening, onions were selected for further study.  A fatty acid fraction separated from yellow onion was subjected to thin layer chromatographic techniques, UV and IR spectrophotometric analyses.  This silicic acid-chromatographed fraction corresponded to the prostaglandin A1 fraction.  Preliminary identification strongly suggested that this component of onions appeared to be a prostaglandin (PGA1) or a prostaglandin-like compound.


Collagen-mediated platelet aggregation. Effects of collagen modification involving the protein and carbohydrate moieties. In an effort to elucidate the nature of the collagen-platelet interaction, the effects of collagen modification on platelet aggregation have been studied. We have shown that purified rat skin (salt) soluble collagen is effective at about 20 nM in mediating platelet aggregation in human platelet-rich plasma. This concentration is somewhat greater than that required of several skin insoluble collagens (ca. 10 nM). Both the alpha1(I) and alpha2 chains from rat skin soluble collagen produced platelet aggregation, but only at concentrations of about 13 muM and 55 muM, respectively. In contrast, heat-denatured collagen and chains (e.g., 65 muM alpha1(I) and 160 muM alpha2) failed to induce platelet aggregation and to inhibit platelet aggregation by native collagen. Glycopeptides were prepared from human skin insoluble collagen by extended digestion with bacterial collagenase and trypsin, and were purified by gel filtration into two classes. One class of higher molecular weight contained sialic acid, glucosamine, galactosamine, fucose, mannose, galactose, and glucose, and the other of lower molecular weight consisted primarily of a mixture of galactose and galactosyl-glucose units O-glycosidically linked to hydroxylysine-containing peptides. We found that, after the residual tryptic activity contaminating the higher molecular weight fraction was inhibited, neither of the glycopeptide classes produced nor inhibited native human skin insoluble collagen-mediated platelet aggregation at the highest concentration examined (ca. 1-2 mg glycopeptide per ml of platelet-rich plasma). Highly purified samples of the hydroxylysyl glycosides, hydroxylysylgalactose and hydroxylysylgalactosylglucose (Hyl-Gal and Hyl-Gal-Glc, respectively), were prepared from human urine and labeled at galactose using galactose oxidase followed by reduction with tritiated borohydride. Binding studies with platelet-rich plasma showed that, at concentrations greater than 50 nM, Hyl-Gal gives apparent binding to platelets, but there was no evidence of Hyl-Gal-Glc binding to platelets at concentrations up to 250 nM. At concentrations several hundredfold higher than the equivalents present in the minimum concentration of rat skin soluble collagen required for platelet aggregation, neither Hyl-Gal (at 29 muM) nor Hyl-Gal-Glc (at 18 muM) caused platelet aggregation or inhibited platelet aggregation by native collagen. Also, at a concentration of 85 muM (which represents a concentration about two thousandfold higher than the equivalents in the minimum concentration of soluble collagen required for platelet aggregation) the Gal-Glc-containing 36 residue rat skin soluble collagen alpha1(I)cyanogen bromide #5 peptide had no platelet aggregating or inhibiting activity. Modification of at least 90% of the rat skin soluble collagen carbohydrate by mild periodate oxidation had no effect on the platelet aggregating activity. Human skin insoluble collagen was reacted with periodate under the same conditions, and this had no demonstrable effect on its ability to induce platelet aggregation. This indicates that the normal carbohydrate side chains of these collagens are not required for the platelet interaction that produces the release of ADP and other metabolic constituents and leads to aggregation.Thus, collagen-platelet interactions appear to involve at least two distinct binding sites on the platelet plasma membrane. One is a protein binding site that activates platelet aggregation and has high specificity and affinity for the collagen triple-helical fold or perhaps even for a particular amino acid sequence in the triple helix.


The kinetics of intramolecular distribution of 15N in uric acid after administration of (15N) glycine. A reappraisal of the significance of preferential labeling of N-(3+9) of uric acid in primary gout. THE CONCEPT OF AN ABNORMALITY OF GLUTAMINE METABOLISM IN PRIMARY GOUT WAS FIRST PROPOSED ON THE BASIS OF ISOTOPE DATA: when [(15)N]glycine was administered to gouty subjects, there was disproportionately great enrichment of N-(3 + 9) of uric acid, which derive from the amide-N of glutamine. An unduly high concentration of (15)N in glutamine was postulated, and attributed to a hypothetical defect in catabolism of glutamine. Excess glutamine was proposed as the driving force of uric acid overproduction. WE HAVE REEXAMINED THIS PROPOSITION IN FOUR GOUTY SUBJECTS: one mild overproducer of uric acid with "idiopathic gout," one marked overproducer with high-grade but "partial" hypoxanthine-guanine phosphoribosyl-transferase deficiency, and two extraordinary overproducers with superactive phosphoribosylpyrophosphate synthetases. In the last three, the driving force of excessive purine biosynthesis is a known surplus of alpha-5-phosphoribosyl-1-pyrophosphate. Disproportionately high labeling of N-(3 + 9) was present in all four gouty subjects, most marked in the most flamboyant overproducers. The precursor glucine pool was sampled by periodic administration of benzoic acid and isolation of urinary hippuric acid. Similarly, the precursor glutamine pool was sampled by periodic administration of phenylacetic acid and isolation of the amide-N of urinary phenylacetylglutamine. The time course of (15)N enrichment of hippurate differed from that of the amide-N of glutamine. Whereas initial enrichment values of hippurate were very high, those of glutamine-amide-N were low, increasing to a maximum at about 3 h, and then declining less rapidly than those of hippurate. However, enrichment values of hippurate and of phenacetyl glutamine were normal in all of the gouty subjects studied. Thus, preferential enrichment of N-(3 + 9) in gouty overproducers given [(15)N]glycine does not necessarily reflect a specific abnormality of glutamine metabolism, but rather appears to be a kinetic phenomenon associated with accelerated purine biosynthesis per se.In addition, greater enrichment of N-9 than of N-3 on days 1 and 2 provided suggestive evidence for a second pathway for synthesis of the initial precursor of purine biosynthesis, phosphoribosylamine, perhaps utilizing ammonia rather than the amide-N of glutamine as nitrogen donor. In this limited study, the activity of this potential second pathway did not appear to be selectively increased in gout.


The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices.


Analogues of adenosine 3',-5'-cyclic phosphate as activators of phosphorylase b kinase and as substrates for cyclic 3',5'-nucleotide phosphodiesterase.


A kinetic model of a cyclic system for the fluorometric microdetermination of adenosine triphosphatase activity.


Study of the mechanism of renal vacuologenesis induced in the rat by ethylenediaminetetraacetate. Comparison of the cellular activities of calcium and chromium chelates.


Adverse effect of cytosine arabinoside on disseminated zoster in a controlled trial.


Stimulation of yeast RNA synthesis by cycloheximide and 3',5'-cyclic AMP.


ATP requirement for release of adenovirus mRNA from isolated nuclei.


Alkaline phosphatase in vascular smooth muscle.


Angiotensin receptors in smooth muscle cell membranes.


Plasma membrane proteins of normal and Rous sarcoma virus-transformed chick-embryo fibroblasts.


Stimulation of calcitonin release in vitro by purine and pyrimidine nucleotides.


Modification of expression of the testicular feminization (Tfm) gene of the mouse by a "controlling element" gene.


Antisynaptosome antibodies affect synaptosomal permeability to neurotransmitters.


REM sleep deprivation impairs latent extinction in rats.


Long-term results of breast cancer treatment during 1959-1966.


Serum levels of parathyroid hormone in the Zollinger-Ellison syndrome.


The brain scan.

The brain scan.


On the development of insulin sensitivity by mouse mammary gland in vitro.


Effects of adenosine-3',5'-monophosphate and 5-hydroxytryptamine on the secretory activity of the canine testis.


Quantitative differential thermal analysis of small dust samples containing quartz.


Bureau of Mines procedure for analysis of respirable dust from coal mines.


A monumental study--reconstruction of a 1920 granite shed.


Isolation of polypeptides containing the intermolecular cross-link  , '-dihydroxylysinonorleucine from dentin collagen.


Respiratory activation of 2,4-dinitrophenol-stimulated ATPase activity in plant mitochondria.


Suppurative perichondritis of the auricle.


Energy dependent changes in the cytochromes of the mitochondrial respiratory chain.


Interconversion of the A and B forms of ceramide trihexosidase from human plasma.


Nucleoside diphosphatase from pig liver: purification and some properties.


Management of nasopharyngeal angiofibroma.


Gadolinium asa probe of the alkaline earth and ATP-metal binding sites in pyruvate kinase.


Incorporation of glucosamine into rhodopsin in isolated bovine retina.


Superoxide dismutase: a comparison of rate constants.


Immunological studies on glucose 6-phosphate dehydrogenase of rat liver.


Radiation therapy for cancer of the lung: previous experience and definition of current issues.


A clinical trial of cyclophosphamide (NSC-26271) and radiation therapy for oat cell carcinoma of the lung.


Combination chemotherapy and radiation therapy for small cell carcinoma of the lung.


Should initial treatment of small cell carcinoma include systemic chemotherapy and brain irradiation?


Observer variability and reliability in lung cancer diagnosis.


A follow-up survey of out-patients in a medical clinic for Africans at Harare Hospital in Rhodesia.


Arousing effects of trophic hormones in hibernating Citellus lateralis.


Behr's syndrome: familial optic atrophy, spastic diplegia and ataxia.


The problem of the Lennox syndrome.

The problem of the Lennox syndrome.


Microbial transformation of cholesterol into coprostanol. Properties of a 3-oxo- 4-steroid-5 beta-reductase.


Stimulation of ribonucleic-acid synthesis in chick-embryo fibroblasts by exogenous adenosine 3':5'-monophosphate.


Effect of pH on electron-paramagnetic-resonance spectra of ceruloplasmin.


Effect of Bordetella pertussis vaccine on plasma corticosterone level and on ACTH-induced corticosterone secretion in rats.


Electron spin resonance spectra of white, cardiac and various red skeletal muscles of the carp, Cyprinus carpio L.


Effect of prostaglandins on cyclic AMP concentrations in toad bladder and rat kidney.


Shortened duration of drug-induced behavioral excitation in rats with septal lesions.


Reaction of rabbit muscle apo-glyceraldehyde-3-P-dehydrogenase with pyridoxal-5'-phosphate.


The binding of divalent cations by purified gangliosides.


Steroid metabolism in testis tissue. Concentrations of testosterone, pregnenolone and 5 -androst-16-en-3 -ol in normal and cryptorchid rat testis, and in isolated interstitial and tubular tissue.


The characterization of glycolipids derived from long-chain polyprenols: chemical synthesis of  -D-mannopyranosyl dolichyl phosphate.


On the specificity of cyclic AMP action in Escherichia coli.


The unambiguous characterization of dolichol phosphate mannose as a product of mannosyl transferase in pig liver endoplasmic reticulum.


Correlation between (3H)dexamethasone binding to a rat liver cytosol receptor protein and stimulation of RNA synthesis by glucocorticosteroids.


Cholesterol and phospholipid composition of mitochondria and microsomes isolated from morris hepatoma 5123 and rat liver.


Stimulation of RNA synthesis in Cicer arietinum seedlings by cyclic 3':5' adenosine monophosphate.


Ganglioside patterns and phenotypic characteristics in a normal variant and a transformed back variant of a simian virus 40-induced hamster tumor cell line. Ganglioside patterns of a cloned Simian virus 40- (SV40) induced hamster tumor cell (Cl(2)TSV(5)-S), its normal variant (Cl(2)TSV(5)-R) which are Cl(2)TSV(5)-S gradually adapted to grow in the presence of 2 microg/ml actinomycin D and exhibit certain normal phenotypic characteristics, and its back variant (Cl(2)TSV(5)-RR), which are Cl(2)TSV(5)-R cells grown in the absence of actinomycin D for more than 60 passages and which exhibit greater phenotypic similarity to Cl(2)TSV(5)-S cells, have been analyzed. All three cell lines contain N(acetylneuraminyl) galactosylglycosyl ceramide (hematoside, GM(3)), N-acetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosyl ceramide (GM(2)), and a higher ganglioside tentatively identified as disialohematoside. However, Cl(2)TSV(5)-R have more GM(2) than Cl(2)TSV(5)-S whereas Cl(2)TSV(5)-RR contain an intermediate amount of GM(2). The amount of GM(2) is correlative with the activity of UDP-N-acetylgalactosamine: hematoside N-acetylgalactosaminyl transferase in the extract of the three cell lines and with their agglutination by wheat germ agglutinin.


Cell division, ciliary regeneration and cyclic AMP in a unicellular system.


Cell cycle-dependent changes in the surface membrane as detected with (3H)concanavalin A.


The effect of environmental pH on the growth of normal and malignant cells.


Isolation and characterization of revertant cell lines. II. Growth control of a polyploid revertant line derived from SV40-transformed 3T3 mouse cells.


Post-transcriptional regulation of catalase synthesis in rat liver and hepatoma: factors activating and inhibiting catalase synthesis.


Defective cyclic adenosine-3', 5'-phosphate-phosphodiesterase regulation in morphogenetic mutants of Dictyostelium discoideum.


Post-transcriptional regulation of catalase synthesis in rat liver and hepatoma: binding of inhibiting factor(s) with polyribosomes synthesizing catalase.


Synthesis of polyoma proteins in vitro.

Synthesis of polyoma proteins in vitro.


Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.


SV40 DNA: quantitative conversion of closed circular to open circular form by an ethidium bromide-restricted endonuclease.


Clostridium infection after intra-amniotic hypertonic saline injection for induced abortion.


Australia antigen in liver tissue--an immunofluorescent and immunoelectron microscopic study.


Effect of myoinositol and other polyols on  -galactosidase of mammarian tissues.


Primary herpetic gingivostomatitis of adult onset. Clinical, laboratory, and ultrastructure correlations identifying viral etiology.


Effects of PGF2  infusion on human cortisol biosynthesis.


Effects of thyrotropin and N6,02'-dibutyryl cyclic 3'.5'-adenosine monophosphate on prostaglandin levels in thyroid.


Antagonism of prostaglandin-promoted pituitary cyclic AMP accumulation and growth hormone secretion in vitro by 7-oxa-13-prostynoic acid.


Prostaglandin release by the isolated perfused rabbit heart.


The effect of PGE1 on ADP-induced aggregation in refractory platelets.


Renomedullary interstitial cells (RIC), prostaglandins (PG) and the antihypertensive function of the kidney.


Differential inhibition by prostaglandins of the renal actions of pressor stimuli.


Significance of the effects of prostaglandins on renal and adrenal function in man.


An essay: prostaglandins and toxemia of pregnancy.


Inotropic effects of prostaglandins, fatty acids and adenosine phosphates on hypodynamic frog hearts.


Further studies on the antigenicity of some injectable tissue extracts.


Evaluation of the effectiveness of treatment on adult height prognosis in disorders with advanced and retarded bone age. Height and height velocity related to chronological or to bone age.


Improvement of adult height prognosis in precocious puberty by cyproterone acetate.


Uptake and degradation of 3H-phenoxymethylpenicillin in rat liver slices.


The effect of guanethidine pretreatment on transmission in the superior cervical ganglion.


Cyclic AMP as a mediator of the relaxing action of papaverine, nitroglycerine, diazoxide and hydralazine in intestinal and vascular smooth muscle.


Pox in wild birds.

Pox in wild birds.


Toxemia of pregnancy. Mechanism and therapeutic management.


An assessment of "free" and conjugated estriol in uncomplicated and complicated pregnancy plasma.


Abnormal biochemistry in myocardial failure.


Trophoblastic disease in Uganda.

Trophoblastic disease in Uganda.


Primary amenorrhea: a simplified approach to diagnosis.


Clinical and metabolic features of anorexia nervosa.


Exercise of normal muscle: biochemical effects.


Fluorescence studies on polyoma virus.

Fluorescence studies on polyoma virus.


Complementation of theta-toxinogenicity between mutants of two groups of Clostridium perfringens.


Carcinoma of the extrahepatic biliary tract.


A "spot-infection technique" for titration and neutralization of poliovirus.


Serial propagation of varicella virus in primary rabbit kidney cells.


Influence of fasting and hemin on microsomal cytochromes and enzymes.


Interaction of cytochrome c with lipid monolayers.


Characterisation of the surface of bovine milk fat globule membrane using microelectrophoresis.


The isolation and characterization of plasma membrane from cultured cells. V. The chemical composition of plasma membranes isolated from chicken tumors initiated with virus-transformed cells.


Isolation of plasma membranes from Ehrlich ascites tumor cells. Influence of amino acids on (Na++K+)-ATPase and K+-stimulated phosphatase.


Binding of proteins to mycoplasma membranes.


Mobility of spin-labelled sulfhydryl sites in excitable tissue.


Preparation of inverted plasma membrane vesicles from isolated adipocytes.


Spin labels as enzyme substrates. Heterogeneous lipid distribution in liver microsomal membranes.


The effects of long-chain alcohols on membrane lipids and the (Na++K+)-ATPase.


Deoxycytidine uptake by isolated membrane vesicles from Escherichia coli K 12.


The purification of membrane-associated tumour antigens by preparative polyacrylamide gel electrophoresis.


Dibutyryl cyclic adenosine monophosphate enhancement of alpha-methyl-D-glucoside accumulation by kidney cortex slices.


Alveolar adenocarcinoma complicating pulmonary fibrosis due to mitral stenosis.


Effect of guanosine 3':5'-monophosphate on the hydroosmotic activity of adenosine 3':5'-monophosphate in toad urinary bladder.


Factors affecting proton magnetic resonance linewidths of water in several rat tissues.


The control of phosphorylase kinase phosphatase by "second site phosphorylation"; a new form of enzyme regulation.


Isolation of acidic lipoproteins from brain chromatin. Their relation to the acidic nonhistone proteins.


Correlation of substrate specificity of cAMP-phosphodiesterase in Dictyostelium discoideum with chemotactic activity of cAMP-analogues.


Stimulation by prostaglandin E1 of adenosine 3':5'-cyclic monophosphate formation in neuroblastoma cells in the presence of phosphodiesterase inhibitors.


Studies of carcinoembryonic antigen activity of whole and extracted serum in ulcerative colitis. The present study has used a microradioimmunoassay to detect carcinoembryonic antigen(s) (CEA) in whole and extracted serum in 77 patients with ulcerative colitis, two of whom had coexisting colonic carcinoma, and 69 patients with carcinoma of the colon or rectum. Taking 5 ng/ml as the level of positivity, the CEA assay on whole serum showed positive results in patients with ;active' ulcerative colitis (15 of 17) but not in those with ;inactive' disease (0 of 23). In two patients, levels fell during drug-induced remission. Our results also indicated that a positive result for CEA on extracted serum was associated with carcinomatous changes in ulcerative colitis in contrast to uncomplicated ulcerative colitis in which positive results were obtained only with whole serum.


Hypersecretion of zymogen granules in the pathogenesis of cystic fibrosis. In the submandibular saliva of 10 cystic fibrosis subjects and 10 controls the turbidity and elevated calcium, protein, and amylase concentrations of the cystic fibrosis secretions, and precipitation of calcium and phosphate in a ratio consistent with hydroxyapatite have been confirmed. By electron microscopy the centrifuged deposits of the cystic fibrosis saliva were seen to be composed predominantly of round or oval subcellular corpuscles. By comparison with submandibular gland, these corpuscles have been identified as inclusion bodies (spherules) from within zymogen granules. Hydroxyapatite crystals formed on standing in the cystic fibrosis saliva. Polyacrylamide gel disc electrophoresis of the cystic fibrosis centrifuged deposits showed five bands, one of which, band 4, was more prominent in the deposit than in the supernatant gels. Comparisons have been made between these results and other studies and have shown (1) elevated calcium and protein in cystic fibrosis exocrine secretions; (2) simultaneous secretion of calcium and enzymes from salivary glands, stomach, and pancreas; and (3) increased salivary secretion of calcium and protein in response to parasympathomimetic and sympathomimetic drugs. Hypersecretion of calcium-containing zymogen granules is postulated as the cause of obstruction in cystic fibrosis.


Studies on the mechanism of the priming effect of interferon on interferon production by cell cultures exposed to poly(rI)-poly(rC). Interferon induction by poly(rI).poly(rC) in primary rabbit kidney and mouse L-929 cell cultures was markedly increased if the cells were previously treated with homologous interferon. This priming effect has been established with different times of exposure of the cells to poly(rI).poly(rC), and was most pronounced for short pulses of contact of the polynucleotide with the cells (10 s, 1 min). Treatment of the cells with pancreatic ribonuclease immediately after their exposure to poly(rI).poly(rC) brought about a relatively greater reduction of the interferon response in interferon-primed cells than it did in unprimed cell cultures. Priming of the cells with interferon did not increase cell-binding of poly(rI).poly(rC), whether this cell-binding was measured quantitatively (by radioactivity, upon exposure of the cells to radiolabeled polymer) or qualitatively (by antiviral activity, by assaying the cell extract for virus plaque reduction). Similarly, interferon priming did not alter the sensitivity of cell-associated poly(rI).poly(rC) to extraneous ribonuclease treatment. Finally, priming with interferon did not decrease the rate of degradation of cell-bound poly(rI).poly(rC) by cellular nucleases nor did it increase the anti-nuclease potency of the cells. The exact mechanism by which previous exposure of the cells to interferon enhances subsequent interferon production, induced by either synthetic polynucleotides or viruses, has not yet been resolved.


Urinary corticosteriod and diphenylhydantoin metabolite patterns in neonates exposed to anticonvulsant drugs in utero.


Role of zinc in the production of Clostridium perfringens alpha toxin. Clostridium perfringens was found to produce alpha toxin in a synthetic medium containing zinc; in medium containing no zinc, a little toxin was detected in the early logarithmic phase of growth and it disappeared rapidly. No intracellular accumulation of alpha toxin protein occurred whether or not zinc was present in the medium. In zinc-deficient medium, the organisms produced and released into the surrounding medium the protein specifically precipitable with alpha antitoxin in an amount comparable to that of alpha toxin produced in the zinc-containing medium. The protein combined rapidly in some unknown way with zinc to form the active and stable alpha toxin.


Inhibition by lithium of prostaglandin E1 and norepinephrine effects on cyclic adenosine monophosphate production in human platelets.


Interferon responsiveness of rabbit synovial cells and chondrocytes. Rabbit synovial cells (SC), articular chondrocytes (AC), and epiphyseal (EC) chondrocytes were grown in vitro. A portion of the spectrum of human pathogenic viruses infective for the cells was determined. Responses of the cells to different viruses or a chemical inducer of interferon varied, with EC responding vigorously and with high titers, AC responding only to chemical inducer and influenza virus, and SC producing no detectable interferon. All cells supported prolonged growth of influenza virus without showing microscopic cytopathology.


Inhibition of enzymes by human salivary immunoglobulin A. Human whole saliva inhibited bacterial neuraminidases and the inhibition was found to reside in the salivary IgA fraction. Further, salivary immunoglobulin (Ig)A inhibited various bacterial enzymes and toxins: neuraminidases from Streptococcus mitis, Streptococcus sanguis, and Clostridium perfringens, hyaluronidase and chondroitin sulfatase from oral bacteria, diphtheria toxin, and streptolysin O. The inhibitory activity of salivary IgA did not correlate with that of serum on the basis of minimum inhibitory dose. A small amount of salivary IgA was required to inhibit oral bacterial neuraminidases, whereas a large amount was required to inhibit other bacterial neuraminidase. Therefore, it is concluded that the absence of neuraminidase activity of oral bacteria in whole saliva may be due to specific inhibition by salivary IgA.


Visna virus infection of sheep and human cells in vitro--an ultrastructural study.


Ultrastructural characterization of genome of epizootic hemorrhagic disease virus. Purified epizootic hemorrhagic disease (EHD) virus preparations were treated with urea and sodium perchlorate. The viral ribonucleic acid (RNA) released when spread on protein monolayers according to the Kleinschmidt technique was examined by rotary shadow-casting electron microscopy. The viral RNA released by urea treatment had filaments which frequently formed three to five loop-shaped figures of varied length. In 80% of the virus particles the lengths of the viral RNA released were 2.5 to 4.5 mum. The sodium perchlorate-released viral nucleic acid also appeared linear, and about 70% had lengths of 0.1 to 2.0 mum, the longest filament measuring 5.8 mum. Evidence was obtained that EHD virus contains double-stranded RNA as its genetic material and the molecular weight of the EHD viral RNA was calculated to be 12.2 x to 10(6) to 15.1 x 10(6) daltons.


The effect of x-radiation on spermatogenesis and the fertility of Schistocerca gregaria (Forsk.).


Glycerolipid synthesis in rat adipose tissue. II. Properties and distribution of phosphatidate phosphatase. The properties and subcellular distribution of phosphatidate phosphatase (EC 3.1.3.4) from adipose tissue have been investigated. The enzyme was assayed using both aqueous phosphatidate and membrane-bound phosphatidate as substrates. When measured with aqueous substrate, activity was detected in the mitochondria, the microsomes, and the soluble fraction. Mg(2+) at low concentration stimulated the phosphatidate phosphatase from soluble and microsomal fractions but had no effect on the mitochondrial phosphatidate phosphatase. At higher concentration Mg(2+) was inhibitory. In the presence of Mg(2+), the phosphatidate phosphatase from soluble and microsomal fractions was active against membrane-bound phosphatidate. No activity was demonstrated with membrane-bound substrate in the absence of Mg(2+). Mitochondria did not contain activity toward the membrane-bound substrate. The rate of utilization of aqueous phosphatidate was always higher than that of membrane-bound substrate. These results indicate that there are at least two different phosphatidate phosphatases in adipose tissue.


A regional survey of myelin development: some compositional and metabolic aspects. A survey of differences in composition and metabolism of myelin from five areas of the central nervous system was made in brain and spinal cord slices of the rat from 20 days to 20 months postnatal age. Purified myelin from the forebrain areas showed a composition characteristic of immaturity longer than did myelin from the hindbrain and spinal cord. The trend of chemical maturity is in agreement with the anatomical observations that myelination begins in the hindbrain and proceeds rostrally. Myelin recovery per 100-mg slice increased continually from 20 days to 20 months of age, while the uptake of [1-(14)C]acetate into myelin lipid and of [1-(14)C]leucine into myelin protein decreased precipitously with age. Taking into account the continuous increase in myelin during maturation, a calculation was made of the total amount of incorporation of labeled material into lipids or proteins per 100-mg slice for each region at each age. The metabolic characteristics of myelin from the cerebral cortex (including the corpus callosum), the thalamic area, and the cerebellum were very similar, while myelin from brainstem and spinal cord was metabolically more active, especially at the early ages. Synthesis of lipid in the myelin sheath represents about 50% of the lipid synthesis of the whole brain and about 75% of that of the spinal cord. The proportion of myelin-related protein synthesis is much less, probably less than 10% of the protein synthesis occurring in whole brain and about 15% of that in the spinal cord except at early ages.


Regulation by dietary choline of hepatic fatty acid synthetase in the rat. Fatty acid synthetase activity was measured in the high-speed supernatant fraction of liver homogenates from rats fed a semisynthetic diet low in lipotropic factors. If choline was omitted from the diet, a significant increase of fatty acid synthetase activity was observed after two feedings of the deficient diet. Compared with controls, the increase of fatty acid synthetase activity was of a magnitude that could account for the amount of triglyceride accumulating in the hepatic floating lipid fraction. Gas-liquid chromatographic analysis of the floating lipid triglycerides showed an increased content of palmitic acid due to choline deficiency; this increase could be predicted from the increased fatty acid synthetase activity and its known characteristic yield of palmitic acid.


A hypothesis relating lymphocyte phosphorylation and transport of ara-C (cytarabine) to its antiviral activity.


Diffusion models for firing of a neuron with varying threshold.


Contribution of the free energy of mixing of hydrophobic side chains to the stability of the tertiary structure of proteins.


Clostridium welchii enterocolitis in arteriosclerotic patients.


Application of radioimmunoassay to the renin-angiotensin system and to aldosterone.


Peptide hormone binding to receptors: a review of direct studies in vitro.


Measurement of steroid hormones using intracellular receptor proteins.


Application of competitive ligand-binding radioassays to the nucleotides.


The impact of radioimmunoassay on our understanding of human adrenal physiology.


Measurements of renin-angiotensin-aldosterone.


Radioimmunoassay methodology: application to problems of heterogeneity of peptide hormones.


Role of the renal lymph in relation to the renin-angiotensin system in the rat.


Thyrotropin- and prostaglandin E2-responsive adenyl cyclase in thyroid plasma membranes.


Gelatin: a poor substrate for a mammalian collegenase. A rabbit tumor collagenase was purified more than 5000-fold. In this form it degrades native collagen in helical conformation at 37 degrees C, pH 7.6, into two fragments, but it had little capacity to cleave gelatin, an indication of the importance of higher-order structure of substrate for this enzyme in pure form. It is likely that, in vivo, enzymes other than collagenase degrade gelatin polypeptides produced by primary collagenolysis.


Nerve growth factor: relationship to the cyclic AMP system of sensory ganglia. Nerve growth factor and N(6),O(2)' dibutyryl adenosine 3',5'-monophosphate both stimulate neurite elongation by explanted ganglia. However, the addition of nerve growth factor does not lead to increased amounts of adenosine 3',5'-monophosphate in intact ganglia, nor does it stimulate adenylate cyclase activity in broken ganglia cells.


Gamma-aminobutyric acid antagonism in visual cortex: different effects on simple, complex, and hypercomplex neurons. Intravenous bicuculline was used to examine how removing gamma-aminobutyric acid-mediated inhibition affects the visual response properties of single cortical neurons. Simple neurons were depressed and complex neurons showed increase in the vigor and range of responses. Hypercomplex cells were no longer inhibited by elongated stimuli. The results are consistent with present evidence concerning the origin and distribution of inhibitory connections within the cortex.


Mutations in immunoglobulin-producing mouse myeloma cells. Three mouse myeloma cell lines were cloned in soft agar and screened by an antiserum overlay method for variants defective in secretion of the myeloma protein. Variants that had lost the capacity to synthesize heavy chains arose spontaneously at a high rate of about 10(-3) per cell per generation. Such variants lost the capacity to produce light chains at a similarly high rate. After cells were treated with the acridine half mustard ICR-191, variants occurred with an even higher incidence, and some of these synthesized heavy chains differing from that of the parent.


Neural micro- and macrostates.

Neural micro- and macrostates.


Estrogen-receptor interaction.

Estrogen-receptor interaction. The interaction of estradiol with uterine cells involves the association of the hormone with an extranuclear receptor protein, followed by temperature dependent translocation of the resulting complex to the nucleus. During this process, the steroid binding unit of the protein undergoes an alteration, called "receptor transformation," that can be recognized by an increase in its sedimentation rate from 3.8S to 5.2S, and by its acquisition of the ability to bind to isolated uterine nuclei and to alleviate a tissue specific deficiency in the RNA synthesizing capacity of such nuclei. Receptor transformation can be effected in the absence of nuclei by warming uterine cytosol with estradiol. This preparation of transformed complex resembles that extracted from nuclei both in its sedimentation rate (5.3S) and in its ability to bind to uterine nuclei and augment RNA synthesis, properties that are not shown by the native complex. It is proposed that receptor transformation is an important step in estrogen action and that a principal role of the hormone is to induce conversion of the receptor protein to a biochemically functional form.


Mesotheliomata in rats after inoculation with asbestos and other materials. Four experiments in which SPF Wistar rats were inoculated intrapleurally with asbestos or other materials are described. Mesotheliomata were observed in a considerable proportion of animals with all the samples of asbestos used and with a sample of brucite. A few were produced with synthetic aluminium silicate fibres and single ones with barium sulphate, glass powder and aluminium oxide. The risk of developing a mesothelioma at a given time after injection was approximately proportional to the dose. Of the UICC standard reference samples, crocidolite was the most carcinogenic and removal of the oils by benzene extraction did not alter the carcinogenicity of these samples. Chemical properties also seem unlikely to be the main factor producing mesotheliomata but the results support the hypothesis that the finer fibres are the more carcinogenic, and this is additional to the known aerodynamic advantage which the finer fibres have in penetrating to the periphery of the lung.


Pediatric antimicrobial therapy IV.

Pediatric antimicrobial therapy IV.


Human polyomavirus infection in renal allograft recipients. Cytological and virological studies on 74 patients with functioning renal allografts were undertaken to detect polyomavirus infection of the renal tract. Ten patients (13.5%) were excreting polyomavirus. Virus particles were seen in the electron microscope in urine samples from eight patients. B.K. polyomavirus was isolated from four patients. Infection with a different polyomavirus was probable in one patient. Virus isolation was most readily achieved when large numbers of intact virus particles were seen in the urine. Patients who were excreting large amounts of polyomavirus shed numerous inclusion-bearing cells which could be detected by cytology. A serological study showed that the prevalence of B.K. antibody was similar to that found in the general population and 38% of this series of transplant patients had evidence of active infection with B.K. virus.


Growth impairment following irradiation in one of a pair of monozygotic twins.


The effects of inactivity, programmed stimulation, and denervation on the histochemistry of skeletal muscle fiber types.


Effects of atropine, reserpine, 6-hydroxydopamine, and handling on seizure development in the rat.


Drug inhibition of PAH transport from cerobrospinal fluid in rabbits.


Acute antiepileptic effects of 9-tetrahydrocannabinol in rats with kindled seizures.


Evaluation of recovery from nondepolarizing neuromuscular block, using a digital neuromuscular transmission analyzer: preliminary report.


Magnetic resonance methods in membrane research.


Adenyl cyclase activity in non-nucleated red blood cells: evidence for its localization in the reticulocytes.


Depolarization by acetylcholine (ACH activation of triphosphoinositide phosphomonoesterase).


Intracellular enzymes in renal lymph as a measure of anoxic injury of the kidney.


Alkylating esters. VII. The metabolism of iso-propyl methanesulphonate and iso-propyl iodide in the rat.


Ineffectiveness of adrenocorticosteroids and adrenocorticotropin in altering Na-K currents in squid giant axon.


A quantitative investigation of the response to injury of the central nervous system of rats treated with ACTH and triiodothyronine.


On mitochondrial inclusions in granulosa lutein cells of pregnant cows.


Inhibition of cell proliferation caused by oncogenic DNA-polyoma virus.


Induction of active immune state by multinucleate tumour cells in mice.


Splenic cellular responses in vitro after tumour-immunization.


Hair tissue in the diagnosis of marasmus and kwashiorkor.


Congenital multiple hemivertebrae.

Congenital multiple hemivertebrae.


Bone case of the month: fibrosarcoma.

Bone case of the month: fibrosarcoma.


Perspective: mental health.

Perspective: mental health.


Characterization of herpes simplex virus-induced deoxyribonucleic acid polymerase.


N-methyl-2-anilinonaphthalene-6-sulfonyl peptides as fluorescent probes for pepsin-substrate interaction.


Phosphorylation of lysine-rich histone in the isolated perfused rat liver. Effects of glucagon, cyclic adenosine 3':5'-monophosphate, and insulin.


Nucleotide sequence determination of bacteriophage T4 leucine transfer ribonucleic acid.


Electron paramagnetic resonance studies of manganese (II)-pyruvate kinase-substrate complexes.


Mechanisms regulating the concentration and the conformation of progesterone receptor(s) in the uterus.


Characterization of a proposed oxytocin receptor in rat mammary gland.


Substrate-dependent, thiol-dependent, inactivation of pig brain nicotinamide adenine dinucleotide glycohydrolase.


Malate dehydrogenase. XII. Initial rate kinetic studies of substrate activation of porcine mitochondrial enzyme by malate.


Cell-free collagen synthesis on membrane-bound polysomes of chick embryo connective tissue and the localization of prolyl hydroxylase on the polysome-membrane complex.


Affinity chromatography and the purification of estrogen receptors.


Pituitary growth retardation in children.


The effect of high-frequency vibration on the activity of some enzymes (cytochrome oxidase, talase, peroxidase) participating in biological oxidation in experiment.


Immunogenic properties of formalin herpes antigen prepared from cell cultures.


Reaction of mice on infection with mycoplasma and leukosis viruses.


Pituitary apoplexy in a child. Case report.


Attenuation of the morphine EEG continuum following a repeat dose within 16 days: delayed tolerance in the rat.


Behavioural effects of dibutyryl cyclic 3',5' AMP, noradrenaline and cyclic 3',5' AMP in rats.


Isolation of a neurotropic type C virus. A neurogenic paralysis of the lower limb can be induced and serially transmitted in mice by a nontransforming type C virus strain that originated in an embryo of a wild mouse. The virus exerted a neurotropic effect on the anterior horn neurons.


Regulation of phosphodiesterase synthesis: requirement for cyclic adenosine monophosphate-dependent protein kinase. Endogenous cyclic adenosine monophosphate (AMP) and its dibutyryl derivative increase cyclic AMP phosphodiesterase activity in cultured lymphoma cells. This effect is prevented by cycloheximide. A variant population of cells deficient in cyclic AMP-dependent protein kinase contains lower basal phosphodiesterase activity, which cannot be induced by cyclic AMP.


Comparative studies on bacterial 5S ribosomal RNA.


Protein binding of nicotinamide adenine dinucleotide and regulation of nicotinamide adenine dinucleotide glycohydrolase activity in homogenates of rabbit skeletal muscle.


Activity of foot-and-mouth disease virus RNA-dependent RNA polymerase in vitro: inhibition by polyamines and poly(amino acid)s.


Bull semen nicotinamide adenine dinucleotide nucleosidase. VI. Fluorescence studies of the enzyme with reduced nicotinamide adenine dinucleotide.


Ligand-induced conformational states of rabbit liver fructose 1,6-bisphosphatase as revealed by digestion with subtilisin.


Relation between the time at which cytopathic endpoint occurs in infected cultures and the initial infective virus dose, with application to the study of inhibitors of cytopathic effect.


Carbohydrate composition of rat hepatocyte nuclear membrane as compared to normal, Morris hepatoma 7800, and phenobarbital-induced microsomal membranes.


Electron microscopy of porcine cytomegalovirus. Viral crystalline arrays and viral forms in the nasal mucosa of piglets.


Enhancement of immunizing potency of a foot-and-mouth disease vaccine for cattle by treatment of the antigen with formaldehyde.


Growth of rhinovirus type 14. Brief report.


The significance of failure to isolate infectious viruses in cases of subacute sclerosing panencephalitis. Brief report.


A simple viability test of cells producing viral antigen. Brief report.


Physical adsorption of respiratory viruses to polystyrene tubes. Brief report.


Bile duct carcinoma in chronic ulcerative colitis.


Auto-antibodies in cirrhosis and hepatocellular carcinoma.


The chemical diagnosis of ganglioneuroma and neuroblastoma using random specimens of urine.


Lesch-Nyhan syndrome: absence of the mutant enzyme in erythrocytes of a heterozygote for both normal and mutant hypoxanthine-guanine phosphoribosyl transferase.


Electrophoretic properties of hypoxanthine-guanine phosphoribosyl transferase in erythrocytes of subjects with Lesch-Nyhan syndrome.


Transmembrane potentials and phospholipid flip-flop in excitable membrane vesicles.


Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.


Specific cytoplasmic glucocorticoid hormone receptors in lactating mammary glands.


Ascites tumor ribonucleic acid polymerases. Isolation, purification, and factor stimulation.


Specific estrogen receptors in the lactating mammary gland of the rat.


Decline in glucose-6-phosphatase activity during prolonged postfixation washing.


Purification of particulate glucose-6-phosphatase.


Cyclic adenosine 3',5'-monophosphate dependent phosphorylation of ribosomal proteins from bovine anterior pituitary gland.


A survey of the different methods used. Experimental study on the application of some methods found suitable to our country.


Evidence for an angiotensinogen-stimulating factor after nephrectomy.


Inhibition and enhancement of the noradrenaline constrictor response by methylxanthines.


Nonspecific entry of thoracic duct immunoblasts into intradermal foci of antigens.


Nuclear differentiation and nucleic acid synthesis in well-fed exconjugants of Paramecium aurelia.


Carcinogenic effect of N-methyl-N'-nitro-N-nitrosoguanidine with various kinds of surfactant in the glandular stomach of rats.


Effect of adenosine3', 5'-monophosphate on thymidine kinase in tumor cells.


Heterogeneity of ferritin from 3'-methyl-4-(dimethylamino) azobenzene-induced hepatomas.


Mucopolysaccharides of rat ascites hepatoma cells.


Production of growth-and differentiation-stimulating factors for mouse leukemia cells by different cell species.


Immuno-electron microscopic studies on cell surface antigens of Friend virus-induced tumor in the rat.


Multinucleated giant cell formation in cultured cells by murine leukemia virus infection.


Chromosomal aberrations of a lymphoblastoid cell line by experimental infection with Epstein-Barr virus.


Human plasma iron responses following test doses of iron from known sources.


Reconstruction of the primary sequence of a protein from peptides of known sequence.


Smooth muscle-like cells in ovaries of the hamster and gerbil.


Comparison of glucose-6-phosphatase activity in unfertilized and fertilized sea urchin egg fractions.


Fluorescence and electron microscopy of the monoamine-containing cells in the turtle heart.


Mediastinoscopic observations of metastatic spread in pulmonary carcinoma.


A quantitative electron microscopic analysis of myelination in the optic nerve of suckling rats treated with an inhibitor of cholesterol biosynthesis.


Mechanism of enzyme action.

Mechanism of enzyme action.


Toward molecular mechanisms of developmental processes.


The cell surface membrane: biochemical aspects and biophysical probes.


Stimulation of polypeptide synthesis by cyclic 3'-5'-guanosine monophosphate.


Studies on calciferol metabolism. VII. The effects of actinomycin D and cycloheximide on the metabolism, tissue and subcellular localization, and action of vitamin D3.


Effects of 2,4-substituents of deuteroheme upon peroxidase functions. II. Reactions of peroxidases and metmyoglobin with azide, cyanide and fluoride.


The b cytochromes in succinate--cytochrome c reductase from pigeon breast mitochondria.


Receptors for glucocorticoids in lung tissue.


Bull semen nicotinamide adenine dinucleotide nucleosidase. VII. Nonenzymatic basis of apparent transglycosidation with histamine.


Cytochrome c interaction with membranes. II. Comparative study of the interaction of c cytochromes with the mitochondrial membrane.


The effects of actin on the electron spin resonance of spin-labeled myosin.


The activities of pyruvate carboxylase, phosphoenolpyruvate carboxylase and fructose diphosphatase in muscles from vertebrates and invertebrates. 1. The activities of pyruvate carboxylase, phosphoenolpyruvate carboxylase and fructose diphosphatase in crude homogenates of vertebrate and invertebrate muscles are reported. 2. Pyruvate carboxylase activity was present in all insect flight muscles that were investigated: in homogenates of bumble-bee flight muscle the activity was inhibited by ADP and activated by acetyl-CoA, and it was distributed mainly in the mitochondrial fraction. This is the first demonstration of pyruvate carboxylase activity in muscle. However, the activity appears to be restricted to insect flight muscle, since it was not found in other invertebrate or vertebrate muscles. 3. Since the three enzymes were never found together in the same muscle, it is concluded that these enzymes cannot provide a pathway for the synthesis of glycogen from lactate or pyruvate in muscle. Other roles for these enzymes in muscle are suggested. In particular, pyruvate carboxylase may be present in insect flight muscle for the provision of oxaloacetate to support the large increase in activity of the tricarboxylic acid cycle which occurs when an insect takes flight.


Atropine sulphate absorption from an intramuscular injection of a mixture of the oxime, P2S, and atropine in exercising humans.


Effects of Naja nivea venom on nerve, cardiac and skeletal muscle activity of the frog. 1. The effects of 1 mg/ml whole Naja nivea (Cape cobra) venom, and of three different toxins isolated from it, on spinal reflex excitability and cardiac and skeletal muscle activity of the frog (Xenopus laevis), were studied. Isolated spinal cords, sciatic nerve-gastrocnemius muscle units and isolated heart preparations were used.2. Synaptic efficiency and/or motoneurone excitability ceased totally and irreversibly after the application of whole venom within 18.5 minutes. The application of alpha toxin caused polysynaptic reactivation of the motoneurones after the monosynaptic response elicited by electrical stimulation, i.e. it had an excitatory effect, which was retained after washing off the toxin. The effect of beta toxin resembled that of alpha toxin, except that the effect was lost after the toxin was washed off. The delta toxin abolished all nervous reactivity irreversibly and was found to be 2.64 times more effective in this respect than whole venom.3. Responses of the gastrocnemius muscle to electrical stimulation of its motor nerve ceased 10 to 20 min after application of the venom. The muscle usually still responded slightly to direct stimulation for a few minutes. The effect was irreversible. The venom did not only block neuromuscular transmission, but also affected the muscle fibre itself. Nerve conductivity was relatively resistant to the venom; alpha and beta toxins produced similar results but were less effective.4. The application of whole venom (1 mg/ml) to the isolated heart resulted in complete spasm of the ventricle within 3 to 8 minutes. The atria continued contracting long after the ventricle ceased. When the venom was washed off, contractions of the atria were restored, while the ventricle remained irreversibly in a state of spasm. The alpha, beta and delta toxins weakened the contractions of the ventricle, but did not produce spasm. The effect on the ventricle was reversible. Contractions of the atria remained apparently unaltered.


Specificity of adenosine on transmitter output at the neuromuscular junction.


Analysis of labile brain constituents using a technique for the instantaneous fixation of brain tissue in vivo.


Chronic dorsal root section on free amino acid levels in the rabbit spinal cord.


Fixation of a Salmonella minnesota R-form glycolipid on the membrane of normal and transformed rat-embryo fibroblasts.


Evidence for a catalytic-centre heterogeneity of molybdoferredoxin from Clostridium pasteurianum.


The effect of insulin and growth hormone on the flux of tracer from labelled lactate in perfused rat heart.


The flux of pyruvate in perfused rat heart.


Relationships between intracellular contents of nucleotides and 5-phosphoribosyl 1-pyrophosphate in Escherichia coli.


Adenylate-cyclase receptors for adrenergic neurotransmitters in rat cerebral cortex.


The neutral proteases of human granulocytes. Isolation and partial characterization of two granulocyte collagenases.


An antibody survey of children in an Ottawa public school.


Formation of the yeast mitochondrial membrane. 1. Effects of inhibitors of protein synthesis on the kinetics of enzyme appearance during glucose derepression.


Magnetic-resonance studies of the interactions between bovine-serum albumin and surfactants. 1. Nature of binding site.


Magnetic-resonance studies of the interactions between bovine-serum albumin and surfactants. 2. Effect of surfactants on the structure of bovine-serum albumin.


ATP-controlled fatty-acid synthesis in cultured arterial fibroblasts.


Tyrosine substitutions resulting from suppression of amber mutants of iso-1-cytochrome c in yeast.


Confirmation of UAG as a nonsense codon in bakers' yeast by amino acid replacements of glutamic acid 71 in iso-1-cytochrome c.


DNA synthesis in polyoma virus infection. II. Relationship between viral DNA replication and initiation of cellular DNA replicons.


Evolution of transfer RNA.

Evolution of transfer RNA.


An in vitro assay for corticotropin releasing factor(s) using suspensions of isolated pituitary cells.


Autoimmune thrombocytopenia occurring in a young woman with idiopathic haemochromatosis.


Interaction of glucocorticoids with rat liver nuclei. I. Role of the cytosol proteins.


Interaction of glucocorticoids with rat liver nuclei. II. Studies on the nature of the cytosol transfer factor and the nuclear acceptor site.


Purification of (14C) protocollagen and its hydroxylation by prolyl-hydroxylase.


Synthesis of four undecanucleotides complementary to a region of the coat protein cistron of phage fd.


Circular dichroism of liver alcohol dehydrogenase complexes with auramine O.


Effect on coenzyme on the bis(8-anilino-1-naphthalenesulfonate)-linked association of beef muscle lactic dehydrogenase.


Lanthanide ions activate alpha-amylase.

Lanthanide ions activate alpha-amylase.


Cooperativity in human erythrocyte phosphofructokinase.


Spectrophotometric assay of 2',3'-cyclic nucleotide 3'-phosphohydrolase: application to the enzyme in bovine brain.


Elevated levels of acceptor activity of hepatoma transfer ribonucleic acid.


Excitatory effects of 5-hydroxytryptamine in the hippocampal-septal circuit.


Increased shock sensitivity in rats with experimental allergic encephalomyelitis and reversal by 5-hydroxytryptophan.


Bicuculline and picrotoxin sensitive inhibition in interpositus neurones of cat.


A simple preparation of bicuculline methiodide, a water-soluble GABA antagonist.


Short-term changes in the firing pattern of hypothalamic neurons during Pavlovian conditioning.


Subarachnoid versus ventricular perfusion in the rabbit.


Hypothermia--a practical aid in veterinary surgery.


Synthesis and evaluation of the anticancer activity of a new series of methanesulfonates.


Focal rhythmic EEG slowing with temporal lobe tumors. Report of six cases.


The influence of topically applied D-tubocurarine on the taste-induced electrical nerve activity in the frog.


The role of transcortin in the distribution of corticosterone in the rat.


Plasma calcitonin-like activity and urinary cyclic adenosine monophosphate during pregnancy, parturition, and lactation in cows with parturient hypocalcemia.


Lipolytic activity of rat pituitaries in vitro.


Influence of median eminence extracts on neosynthesis and release of ACTH.


Biological ACTH activity of lipid mobilizing peptide fractions from hog pituitaries.


Fatty changes and hepatic cell excretion in avian liver. An electron microscopical study of Kepone toxicity.


Experimental infections with Pasteurella septica (sero-group A) and an adeno-or enterovirus in gnotobiotic piglets.


Lipid characterization of bovine serum lipoproteins throughout gestation and lactation.


Inhibition of urea on the hepatocarcinogensis induced by N, N-dimethyl-4-aminoazobenzene in rats.


Modulation of postural reflexes by voluntary movement. II. Modulation at an inactive joint. We have observed that there is a brief, 400 msec period of widespread facilitation of both the Hoffmann and the tendon-jerk reflexes associated with the initiation of a variety of local, voluntary, isometric efforts. It is suggested that the site of this facilitation lies within the spinal cord. We have also observed that this period is followed, for the Hoffmann reflex, by a period of spinal inhibition. By contrast, the tendon-jerk is facilitated, at a reduced level, for at least another 500 msec by mechanisms probably working at the muscle spindle. These results are discussed in comparison with experiments involving activation of the reflex agonist and antagonist muscles. It is proposed that voluntary control of the locomotor system is organized through separate mechanisms for muscle recruitment and for regulation of the sensitivity of the myotatic reflex loop.


Multiple cerebral gliomas in multiple sclerosis. A patient with multiple sclerosis and a multicentric anaplastic astrocytoma is described. The evidence suggests that the relationship is not coincidental and that the astrocytomas originate in multiple sclerosis plaques.


Comparison of the tonic stretch reflex in athetotic patients during rest and voluntary activity. An electromyographic technique was used to record the tonic stretch reflex (TSR) responses to passive sinusoidal stretching of biceps brachii muscle in athetotic patients. The TSR transmission characteristics measured while the patient was in a relaxed state were compared with those measured while the patient was voluntarily active. The resting TSR and action TSR were found to differ markedly. Sensitivity, pattern, duration, and timing of the TSR responses were all changed during voluntary activity. The results suggest that the TSR in athetosis is functionally reorganized by supraspinal influences during voluntary activity. It is proposed that athetoid movements and action tremor may be a manifestation of abnormal action TSR caused by faulty supra-spinal control during activity.


Significance of plasma lysolecithin in patients with multiple sclerosis: a longitudinal study. Five patients with multiple sclerosis and four normal subjects have been followed for over two years. Plasma phospholipid levels and phospholipid fatty acid composition have been assayed periodically. The increased amount of plasma lysolecithin already described by us has been confirmed in this longitudinal study. A significant increase in saturated fatty acids of lysolecithin has also been demonstrated. In contrast, the lecithin concentration and fatty acid composition do not show any significant variations. The lysolecithin saturated/unsaturated fatty acid ratio was different in the same patients at different times, but no correlation was established between these variations and the clinical symptoms measured using the Kurtzke scale. The possible significance of saturated fatty acid increase in lysolecithin in the pathogenetic mechanisms of multiple sclerosis is discussed.


Renshaw cell activity in man.

Renshaw cell activity in man. The H-reflex elicited in triceps surae by percutaneous stimulation of the posterior tibial nerve was conditioned by stimuli applied through the same electrode. The differential sensitivity of motor and sensory fibres to duration of the stimulus pulse made it possible to condition the H-reflex with either a motor or a sensory stimulus. With both types of conditioning, the H-reflex was inhibited at conditioning-test intervals of 2-3 msec and was then facilitated, the peak of facilitation occurring at 5-8 msec with motor conditioning and 6-10 msec with sensory conditioning. The phase of facilitation was followed by further inhibition. We have concluded (1) that the effects of motor conditioning on the H-reflex result from the discharge of Renshaw cells activated by the antidromic volley in the motor axons, and (2) that the effects of sensory conditioning (at the times used in these experiments) are largely due to the activation of Renshaw cells secondary to the discharge of alpha motoneurones by the conditioning volley.


Some effects of chloramphenicol and ethidium bromide on Tetrahymena pyriformis.


The enzymic synthesis of dihydropteroate and dihydrofolate by Plasmodium berghei.


Two improved methods for the determination of soluble carbohydrates.


An operon for histidine biosynthesis in Streptomyces coelicolor. II. Biochemical evidence.


Cure of the child with cancer.

Cure of the child with cancer.


Clostridial brain abscess and hyperbaric oxygen.


Bell's palsy and its treatment.

Bell's palsy and its treatment.


Biogenic monoamines in the cyclostome and lower vertebrate brain.


Paraneoplastic syndromes. A clinically relevant concept in cancer.


Mechanism of the short-term poststimulatory depression of the cochlear microphonics (hysteresis).


Understanding biochemical pathology of depressive illness. An overview.


Faith healing-Pennsylvania style.

Faith healing-Pennsylvania style.


Granulomatous disease of the lungs.

Granulomatous disease of the lungs.


The radiology of bacterial and viral pneumonias.


The radiographic presentation of pulmonary opportunistic inflammatory disease.


Bronchographic criteria of inflammatory disease and radiologic lung biopsy techniques.


Homeostasis and the prostaglandins.

Homeostasis and the prostaglandins.


Proline hydroxylase in injury-induced arteriosclerosis.


Polyol pathway activity in aorta.

Polyol pathway activity in aorta.


Clinical amoebiasis syndrome and case reports of a few unusual cases of amoebiasis.


Netherlands Society for Microbiology meeting at Delft on 25 October 1972. Homologies in the amino acid sequences of cytochrome c-555 from the green photosynthetic bacteria "Chloropseudomonas ethylica" and Chlorobium thiosulfatophilium.


The identification and semi-quantitative assay of some fat-soluble vitamins and antioxidants in pharmaceutical products and animal feeds by thin-layer chromatography.


Hormones and gene expression.

Hormones and gene expression.


Effects of ACTH, adrenalectomy and time of day on emotional activity of the rat.


Lymphocyte activation: the dualistic effect of cAMP.


Cyclic 3',5'-AMP phosphodiesterase of Neurospora crassa.


A novel monosialoganglioside synthesized by a rat brain cytidine-5'-monophospho-N-acetylneuraminic acid: galactosyl-N-acetylgalactosaminyl-galactosyl-glucosylceramide sialyltransferase.


Quiescent SV40 virus transformed 3T3 cells in culture.


The activation of adenylate cyclase. II. The postulated presence of (A) adenylate cyclase in a phospho (inhibited) form (B) a dephospho (activated) form with a cyclic adenylate stimulated membrane protein kinase.


The occurrence of fluoride stimulated membrane phosphoprotein phosphatase.


Synthesis of the E. coli pyruvate dehydrogenase complex: non-dependence on 3'-5'-cyclic AMP.


Evidence for RNA-linked nascent strands in polyoma virus DNA replication.


Decreased binding of insulin to its receptors in rats with hormone induced insulin resistance.


A 5'-amino analog of adenosine diphosphate.


Studies on the in vitro effects of insulin on glycogen synthesis and ultrastructure in isolated rat liver hepatocytes.


The messenger-directed synthesis of the alpha-fragment of the enzyme beta-galactosidase.


Changes of the activities of adenyl cyclase and cAMP-phosphodiesterase and of the level of 3'5' cyclic adenosine monophosphate in rat mammary gland during pregnancy and lactation.


Kinetic and molecular characteristics of allosteric pyruvate kinase from muscle tissue of the sea mussel Mytilus edulis L.


Glucose-6-phosphatase activity in a soluble fraction from cotyledon tissue of Brassica nigra.


Effects of sulfhydryl reagents on synthetic and hydrolytic activities of multifunctional glucose-6-phosphatase.


Properties of adenylate cyclase in mucosal cells of the rabbit ileum and the effect of cholera toxin.


Collagenase activity in homogenates of the involuting rat uterus.


Effect of spermine on the reaction catalyzed by threonyl-tRNA synthetase from rat liver.


Comparative morphometric study of the human pancreas in its normal state and in primary chronic calcifying pancreatitis.


Effect of pyrazole on ACTH stimulated lipolysis "in vivo".


Prostaglandin E1 and platelets.

Prostaglandin E1 and platelets.


Study of steatosis induced in the rat by orotic acid.


Prognosis of early bronchogenic carcinoma. Survival curves of 451 patients after resection of lung cancer in relation to the results of pre-operative tuberculin skin test.


Direct stimulant effect of cyclic AMP and dibutyryl cAMP on glycogenolysis by hepatocytes in histiotypic culture.


Activation of endogenous C-type oncornavirus by 5-bromodeoxyuridine in cultured embryonic rat fibroblasts.


Immunological responses of rabbits artificially infested with the cattle grubs Hypoderma bovis (L.) and H. Lineatum (De Vill.) (Diptera: Oestridae).


Experimental genital herpes infection of the marmoset.


Production of non-defective and defective oligomers of viral DNA in mouse 3T3 cells transformed by a thermosensitive mutant of polyoma virus.


Nuclear magnetic resonance study of exchangeable protons in ferrocytochrome c.


Defective interfering particles of poliovirus. II. Nature of the defect.


Defective interfering particles of poliovirus. 3. Interference and enrichment.


Localization of free radicals on irradiated deoxyribonucleohistone.


Renal failure, uncontrollable thirst, and hyperreninemia. Cessation of thirst with bilateral nephrectomy.


Snoring.

Snoring.


Computerized axial tomography of the head: the EMI-scanner, a new device for direct examination of the brain "in vivo". Special article.


Cochlear summating potentials. Descriptive aspects.


Vestibular disturbances in clinical otosclerosis.


Intraocular penetration of Ara A and idu-therapeutic implications in clinical herpetic uveitis.


Ophthalmic ointment.

Ophthalmic ointment.


Cyclic AMP and calcium ions in mechanical and metabolic responses of smooth muscles; influence of some hormones and drugs.


Adrenergic, cholecystokinetic and morphine-induced effects on extra-hepatic biliary motility.


The metabolic role of lecithin: cholesterol acyltransferase: perspectives form pathology.


Surgery on only-hearing ears.

Surgery on only-hearing ears.


Acute renal failure in obstetric septic shock. Current views on pathogenesis and management.


Detection of a single, metal-independent aminopeptidase activity in polymyxin-extracts from Escherichia coli B.


Roentgen diagnosis of naso-pharyngeal masses.


Chronic marijuana usage and sleep-wakefulness cycles in cats.


Studies on dream recall in chronic schizophrenic patients after prefrontal lobotomy.


L-dopa and REM sleep in normal and mentally retarded subjects.


Effects of alpha-methyltyrosine on REM sleep and brain amine levels in the cat.


A high activity divalent cation ATPase from the distal, caudal epididymis of bulls.


Alkylated pyrimidine derivatives as antiviral agents. 3. Synthesis and antiviral effect of 5-ethyluracil nucleosides.


Alternate immunoprophylactic and biologic techniques in the control of viral respiratory tract diseases.


Lipids in viruses.

Lipids in viruses.


Respiratory syncytial and adenovirus infections of the lower respiratory tract in infanCY.


Chronic renal failure.

Chronic renal failure.


Conversion of angiotensin 1 to angiotensin 2 in the isolated perfused dog lung.


Starvation and human slow-wave sleep.

Starvation and human slow-wave sleep.


Observations on the growth of varicella-zoster virus in human diploid cells.


Homogeneous murine myeloma protein-315 and spin-labeled DNP as a model system for spin-labeled hapten titration technique and spin immunoassay.


Radioimmunoelectroosmophoresis, a technique combining immunoelectroosmophoresis with autoradiography; applications to virology.


X-ray of the month.

X-ray of the month.


When should you consider a cortisone prep?


Effect of catecholamines, histamine and oxyfedrine on isotonic contraction and cyclic AMP in the guinea-pig heart.


Cross-species transmission of mammalian RNA tumor viruses.


Methods of differentiation between virulent and attenuated strains of infectious canine hepatitis.


Response of the eye to a flash of light.


Structure-activity relationships for inhibitors of phosphodiesterase from erythrocytes and other tissues.


Absent posterior arch of the atlas.

Absent posterior arch of the atlas.


Pseudogout.

Pseudogout.


Purine nucleotide synthesis in lymphoblasts cultured from normal subjects and a patient with Lesch-Nyhan syndrome.


Rat uterine contractility and the activities of uterine adenyl cyclase and phosphodiesterase during the estrus cycle.


Muscle tone abnormality and tonic vibration reflex (TVR).


Chemotherapy of choriocarcinoma and related trophoblastic tumors in women.


The kinetics of transmitter release in myasthenia gravis. 3. An electrophysiological analysis of the release of transmitter.


Modulation of adenyl cyclase action in toad bladder by chlorpropamide: antagonism to prostaglandin E.


The action of reserpine on teleost melanophores.


Roy Hertz (1909- ).

Roy Hertz (1909- ).


Studies on the bradycardia induced by (-)- -trans-tetrahydrocannabinol in anesthetized dogs.


Interaction of vasoactive agents and micro-emboli on the pulmonary circulation.


Acute effects of morphine on dopamine synthesis and release and tyrosine metabolism in the rat striatum.


Overview of gestational trophoblastic neoplasia.


Cardiogenic shock. Recent advances in pharmacologic management. II.


Multiplication, O2 consumption and cytochrome oxidase activity of a cold-adapted L cell line.


Synthesis of 2-hydroxy-ethynylestradiol and 4-hydroxy-ethynylestradiol.


The relationship between the viruses causing hepatitis and autoimmunity in liver disease.


Syndactyly in a German shepherd dog.

Syndactyly in a German shepherd dog.


Intranuclear fibrillar bundles in functioning beta-cell adenoma of pancreas.


Effect of cyclic 3'-5'-adenosine monophosphate and theophylline on renal function.


Reinvestigation of the anaerobic glycolytic potential of the regenerating rat liver.


A review of clinical applications and methods for cholinesterase.


Isoelectric focusing of human fetoprotein: an aid in purification and characterization of microheterogeneity.


Circular dichroism studies of the conformation of synthetic peptides in the carboxyl-terminal region of cytochrome c.


Yeast inorganic pyrophosphatase. II. Magnetic resonance and steady-state kinetic studies of metal ion and pyrophosphate analog binding.


Proton magnetic resonance studies of horse cytochrome c.


A molecular weight determination of the 16S ribosomal ribonucleic acid from Escherichia coli.


Magnetic resonance studies of substrate and inhibitor binding to porcine muscle adenylate kinase.


Effects of deoxyribonucleic acid and histone on the number and length of chains of poly(adenosine diphosphate-ribose).


Kinetic effects of inorganic pyrophosphate analogs on several inorganic pyrophosphate hydrolyzing enzymes.


Estradiol receptors in benign and malignant disease of the breast.


Free radicals and neoplasia--some experimental findings and some possible implications.


Calcium and gastrointestinal secretion.

Calcium and gastrointestinal secretion.


Studies on the bacteriophage MS2. An internal nucleotide fragment resembling some ribosomal binding sites.


Oxidative phosphorylation in bacteria which contain different cytochrome oxidases.


Studies on the mechanism of the oxygen-induced synthesis of cytochrome oxidase in yeast. Specific effects of benzimidazole on cytochrome-oxidase anabolism.


The effect of fluoride on the spectral and catalytic properties of the three copper-containing oxidases.


Microbial assimilation of methanol. The ethanol- and methanol-oxidizing enzymes of the yeast Candida boidinii.


Subcellular location of superoxide dismutase in spinach leaves and preparation and properties of crystalline spinach superoxide dismutase.


Solubility requirements for cholesterol esters in human plasma: role of high density lipoprotein protein and phospholipids.


An enzyme variation in a case of congenital methemoglobinemia.


Zollinger-Ellison syndrome.

Zollinger-Ellison syndrome.


Infective agents in the causation of Hodgkin's disease. A review of the epidemiological evidence.


Afferent lung denervation by brief inhalation of steam.


Isomer subfractionation effects in the study of longer chain monoethylene fatty acids in hydrogenated oils.


The component triglycerides of rat adipose tissue. I. As studied after fractionation into classes by silver ion-thin layer chromatography.


Note on the activity of angiotensinase in exercise proteinuria.


Whipple's disease.

Whipple's disease.


Electron microscope studies on aldehyde-fixed, unstained microbial cells.


Studies on the antilipolytic effect of adenosine and related compounds in isolated fat cells.


Frequency dependent decrease of quantal content in a drug-treated neuromuscular junction.


Michaelis-Menten kinetics of secretion of sympathetic neurotransmitter as a function of external calcium: effect of graded alpha adrenoceptor blockade.


Effects of antilipolytic drugs on cyclic 3',5'-AMP dependent protein kinase.


Micropuncture investigations on the effect of angiotensin on the inorganic phosphate reabsorption in the proximal tubule of the rat.


Copper homeostasis in the mammalian system.


Microphysiology of vertebrate neuromuscular transmission.


Histochemical studies on thyroid adenoma built of C cells.


The mode of action and clinical use of spirolactones.


Spontaneous and virus induced transformation in cell culture.


Brain tumors during the first year of life.


Tissue response to a single intraperitoneal injection of various substances in rats.


The inhibitory effect of diethylaminoethyl diphenylvalerate (SKF 525-A) on glucuronidation by cultures of rat hepatoma cells.


Disorders due to enzyme defects in the red blood cell.


Biology of the trophoblast.

Biology of the trophoblast.


Tumours of the central nervous system in Zambia.


Enterotoxic diarrheal syndromes.

Enterotoxic diarrheal syndromes.


Prostaglandins and asthma.

Prostaglandins and asthma.


Insulin receptors.

Insulin receptors.


Review of reviews.

Review of reviews.


Experimental studies on cobalt cardiopathy.


Cytology of the corpus luteum.

Cytology of the corpus luteum.


The mystique of the herpesviruses.

The mystique of the herpesviruses. Probing the biologic mystique of the herpesvirus family, we find elements of antigenic similarity, the capacity of virions to persist within our cells in complete form, and the potential at some later time for these virions to undergo renewed replication. A venereal mode of transmission from one host to another may be characteristic of some of these viruses. Similar patterns of illness may be produced by several of the herpesviruses. Within the cellular framework of inflammation and necrosis which infective virus may evoke in the host, there may also reside the capability of neoplastic transformation.


Some interface phenomena in parasitic protozoa and platyhelminths.


A clinical comparison between monochrome color film and black and white RP-film as a universal x-ray film. A preliminary report.


An electron microscopic study of synaptic organization in the medial superior olive of normal and experimental chinchillas.


The N6-(dimethylamino)methylene derivative of 9-beta-D-arabinofuranosyladenine as an antiviral agent.


Bis-basic-substituted polycyclic aromatic compounds. A new class of antiviral agents. 1. Bisalkamine esters of fluorenone-, fluorenol-, and fluorenedicarboxylic acids.


Regeneration of the endometrium in rabbits after curettage. II. Pretreatment with progesterone.


Regeneration of the endometrium in rabbits after curettage. I. Pretreatment with estrogens.


Phenocopies.

Phenocopies.


Malignant uterine mesotheliomas in squirrel monkeys following diethylstilbestrol administration. 10 adult female squirrel monkeys were implanted subcutaneously with 4 60 mg pellets of diethylstilbestrol (DES); 4 animals were implanted with cholesterol pellets to serve as controls.  Animals were killed at 5, 9, 11, and 14 months after insertion of pellets.  All animals implanted with DES had evidence of extreme estrogenic stimulation, including marked edema of the labia, heavy vaginal cornification, and enlargement of the uterine corpus.  There were several stages of uterine lesions, in general in proportion to the duration of treatment.  The less advanced lesions were restricted to hyperplasia and hyperthrophy of the serosal cells.  In more advanced lesions the myometrium was infiltrated; in the most advanced cases there was invasion of the endometrium.  The 7 most advanced uterine tumors were classified as malignant mesotheliomas.  Cholesterol-treated controls did not have any significant lesions.  These observations indicate that DES is carcinogenic in the squirrel monkey, as it has been demonstrated to be in other lower mammalian species.


Use of neotropical rodents in protein efficiency studies.


An automatic data processing system for long-term toxicological study utilizing the beagle dog.


Serum thyroxine levels in the perinatal guinea pig (Cavia porcellus).


Tuberculin testing in rhesus monkeys (Macaca mulatta): a comparative study using experimentally sensitized animals.


Stability of resistance-susceptibility characteristics in 2 inbred mouse strains.


Characterization of a fibroma virus isolated from naturally-occurring skin tumors in domestic rabbits.


Neoplasms and proliferative lesions in 1065 nonhuman primate necropsies.


Edentate diets. I. Armadillos.

Edentate diets. I. Armadillos.


Edentate diets. II. Two-toed sloths.

Edentate diets. II. Two-toed sloths.


Repair of a mandibular fracture in an irradiated monkey using intraoral immobilization and intraosseous metal mesh fixation.


A vacuum-assisted technic for repetitive blood sampling in the rat.


Technic for collecting blood from neonatal rats.


The use of succinylcholine in the handling and restraint of rhesus monkeys (Macaca mulatta).


Spontaneous Lancefield group G streptococcal infection in a random source cat colony.


A simplified technic for preparing sterile chicken serum.


A clinical laboratory study of conditioned mongrel dogs and Laborador retrievers.


Comparative organ weights for 8 standardized wild rodent species.


Baseline blood determinations of the Taiwan macaque (Macaca cyclopis).


A case of spontaneous congenital pyloric stenosis in a rabbit.


Long-term rabbit restraint--a simple method.


Enzyme induction by viruses.

Enzyme induction by viruses.


Neurotropic viruses and the developing brain.


Purification of 9 -tetrahydrocannabinol, an active constituent of marihuana, by accelerated microparticulate gel chromatography.


Electroencephalography.

Electroencephalography.


On the pathogensis of renovascular hypertension.


Adrenal response to synthetic adrenocorticotrophic hormone during pregnancy and after delivery, with special reference to pre-eclamptic and hypertensive pregnancy.


Long-chain acyl-CoA synthetase activity in blood platelets and some human tissues.


Bluetongue virus.

Bluetongue virus.


Biological significance of molecular chirality in energy balance and metabolism.


The results of prophylaxis of endemic cretinism with iodized oil in rural Andean Ecuador.


Increased aldosterone secretion during sodium depletion with inhibition of renin release.


Some parameters relevant to affinity chromatography on immobilized nucleotides. 1. The suitability of cellulose and Sepharose as supports for affinity chromatography of two groups of cofactor-linked enzymes, dehydrogenases and kinases, was examined. Sepharose was found to be superior. 2. The selective capacities of the columns were measured by frontal analysis and are discussed in relation to the nucleotide contents. 3. The effect of various concentrations of enzyme and of non-specific protein on the performance of the affinity columns, and the effects of equilibration time, flow rate, sample volume and dilution of the nucleotide were examined. 4. The effect of interposing polymethylene and polyglycine extension arms between the matrix backbone and the nucleotide was investigated for several cofactor-dependent enzymes. Maximum binding was observed with an extension arm 0.8-1nm long.


Affinity chromatography of lactate dehydrogenase on immobilized nucleotides. The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immobilized NAD(+) was investigated. The interaction of both lactate dehydrogenase isoenzymes with immobilized pyridine and adenine nucleotides and their derivatives were measured. The effects of these parameters on the interaction of lactate dehydrogenase with immobilized nucleotides were correlated with the known kinetic and molecular properties of the enzymes in free solution.


Adenosine 3':5'-cyclic monophosphate-binding protein from the cellular slime mould Dictyostelium discoideum. During the differentiation of myxamoebae of Dictyostelium discoideum strain Ax-2 grown in axenic medium there is a seven- to ten-fold increase in the specific activity of cyclic AMP-binding protein(s). Evidence is presented for the belief that cyclic AMP phosphodiesterase and cyclic AMP-binding protein are distinct molecular species.


The mechanism of inhibition of rat liver tryptophan pyrrolase activity by 4-hydroxypyrazolo(3,4-d)pyrimidine (Allopurinol). 1. Allopurinol (4-hydroxypyrazolo[3,4-d]pyrimidine) selectively inhibits the apotryptophan pyrrolase activity in homogenates of rat liver in vitro and after intraperitoneal administration. The inhibition is abolished by an excess of haematin. The allopurinol metabolite alloxanthine has no effect on the pyrrolase activity in vitro or after administration. Allopurinol also inhibits the activation of the enzyme in vitro by ascorbate, ethanol plus NAD(+), NADH, hypoxanthine or xanthine. It is suggested that these agents cause the conversion of a latent form of the pyrrolase into the apoenzyme, and that xanthine oxidase is not involved in this process. 2. The raised total pyrrolase activity observed after the administration of cortisol, cyclic AMP, tryptophan, salicylate or ethanol is lowered by allopurinol in vitro to the corresponding holoenzyme values. A similar effect is observed when allopurinol is administered shortly before cortisol or cyclic AMP. Pretreatment of rats with allopurinol completely prevents the enhancement of the pyrrolase activities by tryptophan, salicylate or ethanol. 3. It is suggested that allopurinol inhibits rat liver tryptophan pyrrolase activity in vitro and after administration by preventing the conjugation of the apoenzyme with its haem activator. The possible usefulness of combined allopurinol-tryptophan therapy of affective disorders is discussed.


Inosine 5'-monophosphate dehydrogenase of Escherichia coli K12: the nature of the inhibition by guanosine 5'-monophosphate. When IMP is the variable substrate, IMP dehydrogenase (EC 1.2.1.14) gives non-linear Lineweaver-Burk plots in the presence of GMP. At 0.1mm-GMP the Hill coefficient n=1.15 and at 0.2mm-GMP n=1.34. In the absence of GMP n=1.0. The fact that n exceeds 1.0 in the presence of GMP indicates that the enzyme possesses regulatory (allosteric) properties. The inhibition by GMP is competitive with respect to IMP and non-competitive with respect to NAD(+).


Infection with E.B. virus.

Infection with E.B. virus.


Late clinical and roentgen observations following Thorotrast administration.


The radiological and scintigraphic investigation of spontaneous hypoglycaemia.


Sensitivity to mutagens of Rumex acetosa chromosomes.


Inhibition of angiotensin-coverting enzyme by analogs of peptides from Bothrops jararaca venom.


Enhancement of ethylemethane sulfunate-induced frequency in Drosophila by dimethyl sulphoxide.


Phosphatases. IX. Differences in sialic acid content of rat liver alkaline phosphatase isoenzymes.


Isolation of different thermophilic enzymes from Bacillus stearothermophilus.


Enzyme-polymer adducts of aldolase, glyceraldehydephosphate dehydrogenase, and fructosediphosphatase.


Effect of chemical sympathectomy on pressor responses to norepinephrine, angiotensin and tyramine.