Gentamicin blood agar used as a general-purpose selective medium. The potential value of a blood agar medium containing a final concentration of 5.5 mug of gentamicin per ml was assessed in a diagnostic laboratory over an 8-week period. The medium gave increased isolation rates of beta-hemolytic streptococci, other streptococci, Bacteroides, clostridia, and yeasts. It also proved valuable in detecting gentamicin-resistant gram-negative bacilli when these were present in heavy mixed culture.


Hormone producing tumors of the lung.

Hormone producing tumors of the lung.


Changes in the acetylcholine content in the rat brain after lesions of the septum, fimbria and hippocampus.


Some observations on the pathway of the vestibular rapid transmission system.


Uptake of ( 14 C)glutamate into dorsal and ventral roots of spinal nerves of the rat.


The effect of cyclic N-2-O-dibutyryl-adenosine-3',5'-monophosphate, adenosine triphosphate and butyrate on the body temperature of conscious cats.


Origin and central pathways of crossed inhibitory effects of afferents from the masseteric muscle on the masseteric motoneuron of the cat.


Ectopic production of human chorionic gonadotropin in Ugandan patients with hepatocellular carcinoma.


Cytologic diagnosis of lung lesions by bronchial brushing.


Adriamycin--an antitumor antibiotic in the treatment of neoplastic diseases.


Dependence of human erythrocyte PRPP synthetase specific activity on concentration.


Neurospora crassa pyruvate dehydrogenase: interconversion by phosphorylation and dephosphorylation.


The interaction of yeast inorganic pyrophosphatase with bivalent cations and pyrophosphate.


Inhibition of cyclic 3',5'-nucleotide phosphodiesterase in bovine taste papillae by bitter taste stimuli.


In vitro synthesis rat serum lipoproteins and proteins by Morris hepatoma 7777.


In vitro synthesis of rat serum lipoproteins and proteins by Morris hepatoma 7777.


Structure-activity studies of MSH-release-inhibiting hormone.


Biosynthesis of mannan in Saccharomyces cerevisiae, isolation of a lipid intermediate and its identification as a mannosyl-1-phosphoryl polyrenol.


Some observations on the cofactor requirement for partially purified DNA ligase from Escherichia coli.


A histochemical study on some enzyme changes in the kidney, liver and brain after chronic mercury intoxication in the rat.


Antioxidants and carcinogenesis: butylated hydroxytoluene, but not diphenyl-p-phenylenediamine, inhibits cancer induction by N-2-fluorenylacetamide and by N-hydroxy-N-2-fluorenylacetamide in rats.


An improved sulphur assay applied to a problem of isethionate metabolism in squid axon and other nerves.


Cyclic AMP and phosphodiesterase in synaptic vesicles from mouse brain.


Effect of growth hormone on glycogenesis in cerebral cortical slices.


Agarose-gel electrophoresis of soluble proteins from bronchial mucosa and bronchogenic carcinoma.


Intracellular cyclic adenosine 3', 5'-monophosphate and relaxing effects of isoprenaline and papaverine on smooth muscle of intestine.


Interferon induction by bluetongue virus and bluetongue virus ribonucleic acid.


Cells, intracells and schizogeny. 3. Formation of intracells in human amnion cells and SV40 virus-transformed mouse embryo cells cultured in vitro.


Clinical foot-and-mouth disease in the African buffalo (Syncerus caffer).


Marijuana and ethanol: effects on sleep.


Viruses and myocarditis.

Viruses and myocarditis.


Endocrine studies during a 14-day continuous exposure to 5.2 per cent O 2  in N 2  at pressure equivalent to 100 FSW (4 ATA).


Anaesthesia and toxicity of angiotensin and 5-hydroxytryptamine in rats.


Rise in plasma angiotensin II and of blood pressure induced by 5-hydroxytryptamine in rats.


Cuticlin: a noncollagen structural protein from Ascaris cuticle.


Cyclic AMP inhibition of reactions of glyceraldehyde 3-phosphate dehydrogenase.


Reaction of celite-bound fluorescein isothiocyanate with the 50S E. coli ribosomal subunit.


Modification by bovine growth hormone of liver plasma membrane enzymes, phospholipids, and circular dichroism.


Role of modifications in tyrosine transfer RNA. II. Ribothymidylate-deficient tRNA.


Studies of the copper and heme cofactors of pseudomonad L-tryptophan-2,3-dioxygenase by electron paramagnetic resonance spectroscopy.


Studies on the properties of 1,N 6 -ethenoadenine derivatives of various coenzymes.


Virogenic properties of AKR mouse sarcoma cells induced by rous sarcoma virus.


Physicochemical studies of equine infectious anemia virus. V. Effect of ultraviolet irradiation on virus infectivity.


Failure of actinomycin D to inhibit the induction of SV 40 viral antigen in BSC 1  transformed cells.


A new species of poliovirus top component.


Orthophosphate and amino acids as nutritional factors stimulating diethylaminoethyl-dextran-induced cell sensitization to transfection.


Avian sarcoma virus transformed hamster cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity.


Experiences from investigations of virus isolations from sewage over a two year period with special regard to polioviruses.


Lateral phase separations in binary mixtures of cholesterol and phospholipids.


Stimulation in vitro of prostatic ribonucleic acid polymerase by 5 -dihydrotestosterone-receptor complexes.


Phosphodiesterase activity and the potentiation by theophylline of adrenocorticotrophin stimulated steroidogenesis and adenosine 3',5'-monophosphate levels in isolated rat adrenal cells.


Phosphorylcholine: a specific promoter of reparin and serum  -lipoprotein interaction.


Activation of the template activity of isolated rat liver nuclei for DNA synthesis and its inhibition by NAD.


A spin label study of E. coli membrane vesicles.


The sequence and optical configuration of the amino acids xentoxin.


Allosteric and isosteric modifiers of NADH binding to cytoplasmic malic dehydrogenase.


Electrostatic interactions in the binding of fluorescent probes to lipid membranes.


Phospholipid hydrolysis by phospholipase A 1  and A 2  in plasma membranes and microsomes of rat liver.


Isolation and properties of skeletal muscle plasma membrane.


Proteins and glycoproteins in plasma membrane fractions of avian leukosis-sarcoma virus susceptible and resistant chicken embryo fibroblasts.


Structural properties of the membrane of intact human spermatozoa. A study with fluorescent probes.


The use of a PMR probe for studying the hydrophobic properties of Micrococcus lysodeikticus membranes and membrane fractions.


Ouabain binding sites and the (Na + ,K + )-ATPase of brain microsomal membranes.


Heart rate variability during sleep and wakefulness in low-birthweight infants.


The prejunctional actions of some non-depolarizing blocking drugs. 1. Trains of end-plate potentials (e.p.p.s) have been recorded from the isolated tenuissimus of the cat. The muscle was paralyzed either by transversely cutting the muscle fibres or by non-depolarizing blocking drugs.2. The following parameters of transmitter synthesis, storage and release have been calculated: the quantal content of the first e.p.p. in the train, the size of the available store, fractional release, quantum size, and the rate of refilling of the available store.3. Tubocurarine and benzoquinonium depressed the rate of refilling of the available store causing its depletion at high rates of stimulation. This was offset by an increase in fractional release, which in the case of tubocurarine was sufficient for the quantal content of the first e.p.p. to be unchanged.4. Dimethyltubocurarine and pancuronium had a similar effect to tubocurarine on the rate of refilling of the store and depletion of the store at high rates of stimulation but did not increase fractional release. There was, therefore, a decrease in the quantal content of the first e.p.p.5. Lignocaine depressed the rate of refilling of the store and depleted the store at high rates of stimulation. Fractional release was also depressed.6. It is suggested that the non-depolarizing drugs have a weak local anaesthetic action retarding the influx of sodium into the nerve terminal which slows the rate of refilling of the store. This effect is due to the quaternary ammonium head. The presence of a phenolic group increases fractional release due either to an increased influx of calcium into the nerve terminal or to a potentiation of the actions of calcium.


Some pharmacological effects of the nematocide, morantel. 1. In isolated nerve-muscle preparations as well as in nerve-muscle preparations in intact anaesthetized animals, morantel exhibited neuromuscular blocking properties similar to those of depolarizing blockers. The drug also caused spastic paralysis of 3 day-old chicks and contracture of the isolated toad rectus abdominis muscle.2. Morantel caused contraction of the guinea-pig and rabbit isolated small intestine. This effect was antagonized by atropine and hexamethonium.3. Morantel caused an increase in the blood pressure of the anaesthetized rat and cat and contraction of the nictitating membrane of the anaesthetized cat. These effects were antagonized by hexamethonium.4. It was concluded that morantel (like the related compound pyrantel) has acetylcholine-like action and that its structure is consistent with such action.


Effects of an isolated toxin from Australian tiger snake (Notechis scutatus scutatus) venom at the mammalian neuromuscular junction. 1. The acute effects of a purified toxin from Australian Tiger snake (Notechis scutatus scutatus) venom have been investigated at the mammalian neuromuscular junction.2. The toxin was injected into the tail vein of mice. Death was due to respiratory paralysis.3. The resting membrane potential, and action potential of muscle fibres in muscles from in vivo intoxicated animals were normal.4. The frequency of miniature end plate potentials (m.e.p.p.s) from intoxicated nerve-muscle preparations was reduced, although m.e.p.p. amplitude was unaltered.5. Nerve stimulation resulted in end plate potentials (e.p.p.s) of quantal amplitude; only rarely was the e.p.p. large enough to give rise to an action potential.6. High (20 mM) K(+) did not increase m.e.p.p. frequency in intoxicated preparations.7. The toxin was largely ineffective in vitro.8. The similarities and differences between this toxin, beta-bungarotoxin and botulinum toxin are discussed.


Some effects of inhibiting endogenous prostaglandin formation on the responses of the cat spleen. 1. Cat isolated spleens release prostaglandins into the venous effluent in response to stimuli such as nerve stimulation, noradrenaline and angiotensin II.2. The release of prostaglandins is abolished by pre-treatment of the spleen with indomethacin (0.3-5 mug/ml).3. The capsular and vascular responses to the different stimuli are augmented after inhibition of prostaglandin release.4. The prevention of prostaglandin release, as well as the augmentation of vascular and capsular responses, are reversible after indomethacin treatment is stopped.5. The role of prostaglandins as modulators of the responses to several stimuli is discussed.


Pharmacological properties of centrally-administered agents which interfere with neurotransmitter function: a comparison with the central depressant effects of ouabain. 1. Centrally administered sodium diethyldithiocarbamate (DDC) produced hypothermia, central nervous depression and potentiation of the antinociceptive effect of morphine. These effects resemble those seen with centrally administered ouabain. Furthermore, the interactions of (+)-amphetamine, desmethylimipramine and nialamide with DDC and ouabain were similar.2. 6-Hydroxydopamine by the same route also produced central nervous depressant effects including hypothermia, decreased locomotor activity and catalepsy but not ptosis.3. Both ouabain and chlorpromazine produced similar effects on behaviour and body temperature including selective abolition of a conditioned avoidance response.4. Although centrally administered tetrabenazine produced ptosis, decreased locomotor activity and catalepsy, it had no significant effect on body temperature. However, the hypothermia produced by peripherally administered reserpine was reversed by centrally administered dibutyryl cyclic 3',5'-adenosine monophosphate.5. Centrally administered cocaine and desmethylimipramine produced no depressant effects but an increased excitability and responsiveness were apparent in both cases.6. Although the observed behavioural depression and hypothermia can occur independently both seem to involve an interference with dopaminergic systems.


The surgical management of primary hepatocellular carcinoma in Uganda.


Renin-like activity of the rat submaxillary gland: characterization and the effect of several drugs and stimuli. 1. Renin-like activity was found in rat submaxillary glands.2. This activity was destroyed by boiling, was non-dialyzable and showed an optimum at approximately 50 degrees C.3. Renin-like activity in the submaxillary gland was not diminished 24 h after nephrectomy but was considerably reduced after ligature of the submaxillary duct.4. Renin-like activity in the submaxillary gland was reduced following food-deprivation, water-deprivation or hypovolemia.5. Renin-like activity in the rat submaxillary gland was increased after isoproterenol administration but not following pilocarpine.6. Renin-like activity in the rat submaxillary gland was increased considerably by administration of NaCl or KCl, as well as following adrenalectomy.7. Chlorothiazide and ouabain increased submaxillary renin-like activity but diazoxide did not affect this activity.


Effects of exercise, training, and diet on muscle citric acid cycle enzyme activity.


Dog kidney phospholipids and the question of renin inhibition.


Activity and positional specificity of a rat plasma phospholipase on phosphatidylethanolamine.


A spin-labelled fluorescent probe for membranes.


Chromatin low molecular weight RNA components.


Interrelationship between serum  -lipoprotein and cholinesterase.


Translational control of protein synthesis in rat anterior pituitary by N 6 -2'-O-dibutyryl adenosine 3',5'-monophosphate.


Tumor and plasma ACTH concentrations in patients with and without the ectopic ACTH syndrome.


ACTH secretion by bronchial carcinoid tumours.


Steroid 17,20-desmolase deficiency: a new cause of male pseudohermaphroditism.


Herpes simplex encephalitis: a light and electron microscopic study in a fatal case.


Forefoot reconstruction. A case study.

Forefoot reconstruction. A case study.


Autogenous immunity to endogenous RNA tumor virus. Radioimmune precipitation assay of mouse serum antibody levels. The radioimmune precipitation assay using (3)H-labeled AKR leukemia virus was applied to the detection and quantitation of natural serum antibodies directed against endogenous murine leukemia virus (MuLV) envelope antigens B6C3F(1) and BALB/c mice, which have low natural incidences of leukemia and lymphoma, and AKR mice, which have a high incidence, were used in this study. Sera from mice of various age groups were assayed. A marked difference in age-associated levels of the autogenous immune response to endogenous murine leukemia virus was detected, and the quantitative differences among these strains were inversely related to the incidence of lymphoma. The radioimmune precipitation test as applied was 500 times more sensitive than virus neutralization. That the reactions we have observed are specific is suggested by several lines of evidence, including the nonreactivity of normal hamster and absorbed rat serum, the positive reaction of absorbed rat anti-AKR serum, the inhibition of precipitation of labeled virus by purified unlabeled virus, and isopycnic gradient analysis of the reactive products.


Stress relaxation in rat skin after treatment with hormones.


Carcinogenesis in tissue culture. 21. Changes in back-transplantability of the population of rat ascites hepatoma JTC-15 cells during a long-term of cultivation.


Descending and segmental inhibition of transmission through the spinocervical tract. 1. Micro-electrode recordings were made from axons of the spinocervical tract in unanaesthetized decerebrate-spinal cats.2. The effects of stimulation of (1) descending systems at the level of the upper cervical spinal cord and (2) hind limb cutaneous nerves, on discharges of spinocervical tract neurones were examined.3. Effects were obtained from bilateral spinal cord regions in the dorsolateral funiculi and the most medial and ventral parts of the ventral funiculi and also from the dorsal columns in the upper cervical region even though the columns had been transected at low thoracic and upper lumbar levels.4. Stimulation of either descending or segmental systems inhibited spontaneous and evoked responses. Facilitation was not seen. The inhibition had a time course of up to 250 msec, with maximal action at 20-40 msec and was greatest for polysynaptic responses or those evoked from the smaller myelinated cutaneous axons.5. It is suggested that the descending and segmental systems converge on to common inhibitory interneurones.


Influence of chronic neostigmine treatment on the number of acetylcholine receptors and the release of acetylcholine from the rat diaphragm. 1. Rats were treated twice daily for 7 days with neostigmine and the diaphragm was isolated for study of its acetylcholine content, release upon nerve stimulation and the number of receptors in the end-plate.2. While the content of total acetylcholine was unchanged, the release of acetylcholine on stimulation with trains of 500 pulses at 100 Hz every 20 sec was reduced by about 50%. Five days after the end of neostigmine treatment the release of acetylcholine recovered to normal.3. The total number of acetylcholine receptors in the end-plate as measured from the binding of N, O-di[(3)H]acetyl alpha-bungarotoxin was reduced from 2.1 x 10(7) to 1.2 x 10(7) per end-plate.4. The above-mentioned changes are not due to acute pharmacological effects of neostigmine, nor to an immediate effect of cholinesterase inhibition but presumably due to chronic accumulation of acetylcholine at the neuromuscular junction.


Effects of proteolytic enzymes on function and structure of frog neuromuscular junctions. 1. Frog cutaneous pectoris nerve-muscle preparations were incubated with collagenase and protease and examined with electrophysiological and electron microscopic techniques.2. The physiological properties and intracellular ultrastructural appearance of individual muscle and nerve cells were not affected by the enzyme treatment. However, neuromuscular transmission and the morphology of the nerve-muscle junction were altered.3. Collagenase produced an irreversible loss of activity of end-plate cholinesterase and a partial loss of stainable ;synaptic cleft material'.4. Protease produced these changes and, in addition, the entire basement membrane was digested, which led to ;synaptic disjunction' of nerve terminals and muscle end-plates.


Nasopharyngeal carcinoma and Epstein-Barr virus. I. Factors related to the anti-VCA antibody.


Nasopharyngeal carcinoma and Epstein-Barr virus. II. Clinical course and the anti-VCA antibody.


Clostridium perfringens exotoxins. I. Purification and properties of  -toxin.


Mode of action of hallucinogenic molecules.


Adenyl cyclase of polymorphonuclear leukocytes.


Assignment of codons to arginine residues in vertebrate proteins.


Effects of flow-stop on the metabolism of noradrenaline released by nerve stimulation in the perfused spleen.


Alterations produced by cortisol in the spontaneous activity and responsiveness to sensory stimuli of single cells in the tuberal hypothalamus of the rat.


Influence of cyclization and acyl substitution on the inotropic effects of adenine nucleotides.


Studies on the in vitro and the in vivo transcription of the bluetongue virus genome.


Visualization of the extracapsid coat in certain bluetongue-type viruses.


Phagocytic activity of peritoneal exudate cells from mice.


Therapeutic planning for patients with lung cancer.


Lung biopsy: transbronchoscopic, percutaneous, open.


Mediastinal tumors and cysts in the pediatric patient.


Alveolar and tissue phospholipids of rat lung.


Effects of changes in sodium balance and of experimental renal hypertension on plasma angiotensin I converting enzyme activity of rabbits.


The disinfection of instruments and hands during outbreaks of epidemic keratoconjunctivitis.


Treatment of adenovirus type 8 kerato-conjunctivitis.


Pseudomalignant cutaneous amoebiasis in Uganda.


Riboflavin carrier protein from egg yolk. Spectral and other properties observed upon binding of flavin to apoprotein.


The effects of nitrofuranfuradroxyl on the phosphatase enzymes in kidney and liver of rats.


Complex conformations in mitochondrial DNA isolated from rat ascites hepatoma cells.


Probable explanation for differential inhibition of hepatoma growth in liver upon carbohydrate deprivation.


Studies on urinary infections in diabetics. IV. Significant bacteriuria in pregnancy in relation to age of onset, duration of diabetes, angiopathy and urological symptoms.


Studies on arterial and renal venous plasma renin activity in hypertensive patients.


Lipoprotein lipase activity of human adipose tissue in health and in some diseases with hyperlipidemia as a common feature.


Cell size and lipolysis by human subcutaneous adipose tissue.


Primary epithelial neoplasms of the lacrimal sac.


Parenteral long-acting corticosteroids: effect on hypothalamic-pituitary-adrenal function.


A new short-acting antagonist of angiotensin II.


Influence of vasoactive hormones on mammary arterial blood flow in lactating cows.


Mechanism of acetylcholine release by drugs and its blockade.


Effect of sulfur compounds on various sulfhydryl-dependent enzyme systems.


Interaction of lipids with acid-soluble bovine skin collagen.


A philosophy of relaxation. Eleventh annual Baxter-Travenol lecture.


Analysis of human hypoxanthine-guanine phosphoribosyl transferase isozymes by isoelectric focusing in polyacrylamide gel.


Neonatal diseases in dogs and cats.

Neonatal diseases in dogs and cats.


Chronic benign familial pemphigus. Induction of lesions by Herpesvirus hominis.


Pulmonary cavitary lesion with productive cough.


Cervical chemodectoma with extensive pulmonary metastases.


Do maternally administered neuromuscular blocking agents interfere with fetal neuromuscular transmission?


Delayed granuloma following bronchography.


Mediastinal xanthoma with familial hyperlipoproteinemia.


Clinical staging of primary lung cancer.


Wilms tumor: diagnosis by thoracentesis.


The spectrum of diffuse pulmonary infiltration in malignant disease.


Clinical significance of hemodynamic findings in hypertension.


ACTH and calcitonin secreting medullary carcinoma of the thyroid.


The effect of renal failure and hyperkalemia on the duration of pancuronium neuromuscular blockade in man.


The effect of chlorpromazine on pituitary function.


A radioimmunoassay for MTV-antigens.

A radioimmunoassay for MTV-antigens.


Hydrolytic enzymes of the hepatopancreas of the ascidian, Pyura stolonifera.


Carcinoma associated with fistula in ano.


Gas gangrene of the anterior abdominal wall.


Histidyl-transfer ribonucleic acid synthetase mutants requiring a high internal pool of histidine for growth. Mutants that require histidine due to an altered structural gene for the histidyl-transfer ribonucleic acid synthetase (hisS) have been isolated by a general selection for histidine-requiring strains in which the mutation producing histidine auxotrophy is unlinked to the histidine operon. One of the mutants has been shown to require an abnormally high internal histidine pool for growth owing to an altered synthetase that is unstable at low histidine concentrations. It is difficult to determine accurately the K(m) for histidine of the synthetase enzyme from the mutant because of the instability of the enzyme at limiting histidine concentrations; however, a histidine K(m) value has been estimated that is approximately 100 times higher than the histidine K(m) of the wild-type enzyme. For the mutant strains to achieve the high internal pool of histidine required for growth, all the systems that transport histidine from the growth medium must be functioning to capacity. Amino acids that interfere with histidine transport strongly inhibit the growth of the mutants. The mutants have been useful in providing a selective genetic marker for transductional mapping in the hisS region. The mutants are discussed as representative of a general class of curable mutants that have an altered enzyme with poor affinity for a substrate or coenzyme.


Common enzymes of branched-chain amino acid catabolism in Pseudomonas putida. Two types of Pseudomonas putida PpG2 mutants which were unable to degrade branched-chain amino acids were isolated after mutagenesis and selection for ability to grow on succinate, but not valine, as a sole source of carbon. These isolates were characterized by growth on the three branched-chain amino acids (valine, isoleucine, and leucine), on the corresponding branched-chain keto acids (2-ketoisovalerate, 2-keto-3-methylvalerate, and 2-ketoisocaproate), and on other selected intermediates as carbon sources, and by their enzymatic composition. One group of mutants lost 2-ketoisovalerate-inducible branched-chain keto acid dehydrogenase that was active on all three keto acids. There was also a concomitant loss of ability to grow on all three branched-chain amino acids as well as on all three corresponding keto acids, but there was retention of ability to use subsequent intermediates in the catabolism of branched-chain amino acids. Another type of mutant showed a marked reduction in branched-chain amino acid transaminase activity and grew poorly at the expense of all three amino acids, but it utilized subsequent intermediates as carbon sources. Both the transaminase and branched-chain keto acid dehydrogenase mutants retained the ability to degrade camphor. These findings are consistent with the view that branched-chain amino acid transaminase and branched-chain keto acid dehydrogenase are common enzymes in the catabolism of valine, isoleucine, and leucine.


Genetic and biochemical investigation of the Escherichia coli mutant hfl-1 which is lysogenized at high frequency by bacteriophage lambda. The Escherichia coli mutant hfl-1 is lysogenized at very high frequency by bacteriophage lambda. The normal requirement for the lambdacIII gene product in the establishment of repression is not observed in hfl-1 strains. These phenotypic characteristics are specified by a single locus at 82.5 min on the E. coli map in extremely close proximity to the purA gene, cotransduction frequencies ranging from 97 to 100% depending on the particular purA marker used. The lactose operon is shown to function normally in this strain, and there are also no demonstrable differences in ribonucleic acid polymerase activity or cyclic-adenosine monophosphate levels. Alterations in the cell envelope are indicated by a slight rifamycin resistance, which is reversible by pretreating the cells with ethylenediaminetetraacetic acid, and by a resistance to penicillin and a sensitivity to high concentrations of sodium dodecyl sulfate. It is not known whether this change in cell surface is the primary lesion, or a pleiotropic effect of some more basic metabolic shift.


Relationship of adenosine 3',5'-monophosphate to growth and metabolism of Tetrahymena pyriformis. The concentration of adenosine 3',5'-monophosphate (cyclic AMP) and the activity of adenylate cyclase were determined for the first time in conjuncation with cyclic 3',5'-nucleotide phosphodiesterase (phosphodiesterase) during the growth cycle of Tetrahymena pyriformis. High levels of cyclic AMP observed during early exponential and late stationary phases were associated with elevated adenylate cyclase and decreased phosphodiesterase activities. Adenylate cyclase and cyclic AMP were decreased and phosphodiesterase was increased in cells grown in glucose-supplemented medium. In contrast to findings in mammalian liver, cyclic AMP was decreased during active gluconeogenesis in Tetrahymena. This suggests a different modulation of carbohydrate metabolism in the two species. The results illustrate that both the content of cyclic AMP and its action as a regulatory agent in Tetrahymena are uniquely suited to the metabolism of this organism.


Respiratory development in Saccharomyces cerevisiae grown at controlled oxygen tension. Saccharomyces cerevisiae was grown in batch culture over a wide range of oxygen concentrations, varying from the anaerobic condition to a maximal dissolved oxygen concentration of 3.5 muM. The development of cells was assayed by measuring amounts of the aerobic cytochromes aa(3), b, c, and c(1), the cellular content of unsaturated fatty acids and ergosterol, and the activity of respiratory enzyme complexes. The half-maximal levels of membrane-bound cytochromes aa(3), b, and c(1), were reached in cells grown in O(2) concentrations around 0.1 muM; this was similar to the oxygen concentration required for half-maximal levels of unsaturated fatty acid and sterol. However, the synthesis of ubiquinone and cytochrome c and the increase in fumarase activity were essentially linear functions of the dissolved oxygen concentration up to 3.5 muM oxygen. The synthesis of the succinate dehydrogenase, succinate cytochrome c reductase, and cytochrome c oxidase complexes showed different responses to changes in O(2) concentration in the growth medium. Cyanide-insensitive respiration and P(450) cytochrome content were maximal at 0.25 muM oxygen and declined in both more anaerobic and aerobic conditions. Cytochrome c peroxidase and catalase activities in cell-free homogenates were high in all but the most strictly anaerobic cells.


Metabolism of the pyridine nucleotides involved in nicotinamide adenine dinucleotide biosynthesis by Clostridium butylicum. In order to elucidate the mechanism of the accumulation of considerable amounts of free nicotinic acid (NA) in the culture medium of Clostridium butylicum, this organism was investigated with regard to its ability to metabolize nicotinamide adenine dinucleotide (NAD) and its immediate biosynthetic precursors, nicotinic acid mononucleotide (NAMN) and nicotinic acid adenine dinucleotide (deamido-NAD). Cell-free extracts of C. butylicum were found to degrade NAMN and deamido-NAD to NA. NAMN, in the presence of adenosine triphosphate (ATP), was converted to deamido-NAD, but only at high concentrations of ATP (20 mM) was significant synthetic activity observed in competition with NAMN degradation. Degradation of both NAMN and deamido-NAD was activated by ATP at concentrations of 5 and 10 mm. Anaerobiosis markedly enhanced the degradation of the nucleotides. The data indicate that the synthesis of NAMN and deamido-NAD prevails over their degradation only in the presence of high concentrations of ATP. NAD was degraded to nicotinamide mononucleotide (NMN) by a pyrophosphatase. Phosphate markedly inhibited both the deamido-NAD and NAD pyrophosphatases. Under anaerobic conditions there was practically no further degradation of NMN to NA, whereas barely measurable amounts of NA were formed under aerobic conditions. All of these observations suggest that, under the given conditions of anaerobiosis and physiological phosphate concentrations, there is very little degradation of NAD to NMN and practically no degradation to NA by C. butylicum. Thus, NAD represents an insignificant source of the NA accumulated in the culture medium. The intermediates in the biosynthetic pathway (NAMN and deamido-NAD) have been shown to be the major source of the NA which is accumulated by C. butylicum.


Inhibition by fluoride ion of hormonal activation of fat cell adenylate cyclase.


Fractionation of the C-apoproteins from human plasma very low density lipoproteins.


An iron-containing superoxide dismutase from Escherichia coli.


Effects of ethanol and fat on the transport of reducing equivalents into rat liver mitochondria.


Structure-function unitization model of biological membranes.


Studies on the role of ribosomal proteins L 7  and L 12  in the in vitro synthesis of  -galactosidase.


Comparative biological chemistry of cobalt hemoglobin.


Glucocorticoid receptors in lung. II. Specific binding of glucocorticoids to nuclear components of rabbit fetal lung.


Preparation and characterization of plasma membranes from bovine corpus luteum.


Properties of the luteinizing hormone receptor of isolated bovine corpus luteum plasma membranes.


Perifused fat cells. Effect of lipolytic agents.


The plasma membrane of isolated fat cells. I. Identification of trypsin-sensitive membrane peptides by sodium dodecyl sulfate polyacrylamide gel electrophoresis.


The distribution of carbohydrate groups in rat skin collagen.


The dynamic equilibrium of folding and unfolding of nuclease-T.


Function of phosphorylated ribosomes. The activity of ribosomal subunits phosphorylated in vitro by protein kinase.


Glucagon stimulation of adenylate cyclase sulfhydryl reactivity. Evidence for hormone-induced conformational changes.


Modification of the rat's startle reaction by termination of antecedent acoustic signals.


Positional specificities of acyl coenzyme A: glycerophosphate and acyl coenzyme A: monoacylglycerophosphate acyltransferases in Escherichia coli.


The valence and spin state of iron in oxyhemoglobin as inferred from resonance Raman spectroscopy.


Localization of phosphatase activity on the membranes of the mammalian sperm head.


Neuraminidase "unmasking" and failure of trypsin to "unmask"  -D-galactose-like sites on erythrocyte, lymphoma, and normal and virus-transformed fibroblast cell membranes.


Distinctive cells similar to fetal hepatocytes associated with liver carcinogenesis by diethylnitrosamine. Electron microscopic study.


Anaerobic glycolysis in spontaneous and transplanted liver tumors of mice.


Unusually high incidence of spontaneous lymphomas in wild house mice.


Types B and C RNA virus in breast tissue and milk of wild mice.


Infection of embryonic myogenic cells with murine sarcoma virus in vitro.


Immunologic studies of pertussis. Development of precipitins.


Acceleration of growth and bone maturation in childhood thyrotoxicosis.


Insulin secretion in children with constitutional familial short stature.


Intravenous alimentation in patients with cancer and neutropenia.


Central modulation of baroreceptor reflex by angiotensin.


Complications of influenza and their management.


Sexual differences in the hypothalamic cholinergic system before and after puberty: inductory effect of testosterone.


The relationship between maternal and neonatal sleep.


Abnormalities of carbohydrate and lipid metabolism in experimentally induced acute uremia.


Serum total lipids and lipid fractions in marasmus.


A mutant transcription factor that is activated by 3':5'-cyclic guanosine monophosphate. We report here the isolation of an extragenic suppressor of a promoter mutation in the lactose operon of Escherichia coli. Genetic data have indicated that the suppressor of the P(-) mutation maps in the structural gene for the transcription factor catabolite activator protein. Biochemical studies suggest that the mutant molecules of catabolite activator protein are altered in such a way that they are activated by 3':5'-cyclic GMP in addition to their normal effector, 3':5'-cyclic AMP.


Open-field behavior, habituation and passive avoidance learning: effect of ACTH and hydrocortisone on normal and adrenalectomized rats.


Interaction of amines with ketone aldehydes and unsaturated aldehydes. The optical changes occurring during the interaction of amines with ketone aldehydes or unsaturated aldehydes are accompanied by strong electron spin resonance signals, and can thus be explained as due to the transfer of one whole electron in the ground state.


Nonvirion antigens produced by herpes simplex viruses 1 and 2. Of nine herpes simplex virus 1 strains (from lip, mouth, throat, cornea, or brain) only five produced enough nonvirion antigen (i.e., not a structural component of the virus) to be detected by complement fixation with specially prepared, virion-absorbed, type-1 guinea pig antisera, while the remaining four strains produced only enough of the same antigen to induce specific antibody in hyperimmunized guinea pigs. While the type 1 virion antiserum used reacted equally well by complement fixation with the type 1 and type 2 strains, the type 1 nonvirion antisera failed to react with nonvirion antigens produced by three type-2 (genital) strains. However, type 2 nonvirion antiserum reacted equally well with the three type 2 and four type 1 nonvirion antigens that were tested. It appears, therefore, that while herpes simplex virus 1 codes only for type 1 nonvirion antigen, herpes simplex 2 codes not only for an immunologically distinct type 2 nonvirion antigen but also for enough type 1 nonvirion antigen to stimulate antibody production for it. Herpes simplex 2 nonvirion antigen exhibited the same properties as type 1, i.e., its activity was lost on storage at 4 degrees for 15 days, it was sedimented by centrifugation at 33,360 x g for 1 hr, and the maximum concentration was found at 3 hr in guinea pig kidney culture cells, but at 24 hr in HEp 2 and rabbit kidney culture cells. Sera from patients with genital lesions caused by herpes simplex virus 2, as well as from randomly selected adults, failed to react with either type 2 or type 1 nonvirion antigens. Accordingly, the basic information is now available to permit the use of these nonvirion antigens to determine the possible role of the herpes simplex viruses in certain human cancers.


Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent. A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.


Evidence for the absence of the G-T-psi-C sequence from two mammalian initiator transfer RNAs. Analyses were performed on the purified initiator tRNA from rabbit liver to test for the presence of Psip and Tp in this molecule. Neither of these nucleotides could be detected after hydrolysis by piperidine, NaOH, or T2 RNase. Similarly digestion with venom phosphodiesterase plus phosphatase failed to release any pseudourdine or ribothymidine. Identical results were obtained with the initiator tRNA from sheep mammary gland. The absence of these nucleosides was confirmed by pancreatic and T1 RNase digestion of the rabbit-liver initiator tRNA. The classical G-T-Psi-C sequence was not detected in this molecule. An A-U-C-G sequence has been identified; it may possibly replace the G-T-Psi-C sequence.


Effects of adrenal glucocorticoids on polyoma virus replication. Treatment of polyoma virus-infected fibroblasts from mouse embryos and virus-infected 3T3 cells with physiological concentrations of the glucocorticoids enhances plaque formation at least 10-fold. The data suggest that this effect results from an increased rate of virus production. Steroid specificity studies indicate that the cytoplasmic receptors that mediate the physiological actions of these hormones are also involved in the present effect.


Amino-acid acceptor activity of the "70S-associated" 4S RNA from avian myeloblastosis virus. The "70S-associated" 4S RNA from avian myeloblastosis virus contains transfer RNA, as determined by aminoacylation. The various amino acids esterified, plus the great number of minor bases found in this "70S-associated" 4S RNA indicate that a sizeable fraction, but not all, of this RNA is transfer RNA.


Transformation of chick-embryo fibroblasts by wild-type and temperature-sensitive Rous sarcoma virus alters adenylate cyclase activity. The activity of the enzyme adenylate cyclase, a component of the plasma membrane, has been determined in chick-embryo fibroblasts and in cells transformed by either Bryan high-titer strain of Rous sarcoma virus (RSV-BH) or a temperature-sensitive mutant of this virus (RSV-BH-Ta). Adenylate cyclase activity is reduced in cells transformed by the wild-type virus and also by the temperature-sensitive mutant when the cells are grown at the permissive temperature (37 degrees ). Transformation results in an altered affinity (K(m)) for the substrate (Mg ATP). The apparent K(m) ATP is 0.23 mM in normal cells and 1.1 mM in cells transformed with wild-type virus. The K(m) ATP of the cells infected with RSV-BH-Ta is 0.67-1.0 mM at 37 degrees and 0.28 mM at 42 degrees . The enzyme from normal cells appears to have two binding sites for Mg(++), one at the catalytic site and a second at a regulatory site. Transformation by RSV-BH or RSV-BH-Ta (37 degrees ) apparently alters this second Mg(++) site. A decrease in adenylate cyclase activity occurs within 10 min after cells infected with RSV-BH-Ta are shifted from 42 degrees to 37 degrees ; the activity falls to one-half that of normal cells 30 min after the temperature shift. Our observations indicate that a viral function lowers cyclic AMP content by lowering the activity of adenylate cyclase, probably through some modification of the plasma membrane.


Activation of prolyl hydroxylase in L-929 fibroblasts by ascorbic acid. The addition of ascorbate to the culture medium of early log-phase mouse fibroblasts (L-929 cells) resulted in a 5-fold increase of prolyl hydroxylase activity. Maximal activity was reached within 2 hr after addition of 5 muM ascorbate. The total amount of enzyme-related antigen did not change on treatment with ascorbate and the activation was shown to be independent of RNA and protein synthesis. The increase in activity caused by ascorbate is therefore due to the activation of a preformed precursor. Enzyme (molecular weight 260,000-300,000) and putative precursor (molecular weight 85,000-105,000) were separated by chromatography on DEAE-Sephadex. Treatment of intact cells with dithiothreitol resulted in an almost quantitative conversion of the enzyme to the smaller inactive protein. When these cells were treated with ascorbate or incubated overnight in fresh medium the enzyme reappeared and precursor concentrations decreased proportionately. Ascorbate may act by bringing about aggregation of enzymatically inactive subunits.


A physiological mechanism for Hebb's postulate of learning. Hebb's postulate of learning envisages that activation or inactivation of extant synaptic contacts in plastic neural networks depends on the synchronous impulse activity of pre- and postsynaptic nerve cells. The physiological mechanism proposed here for this process posits that at synapses acting according to Hebb's postulate, the receptors for the neurotransmitter are eliminated from the postsynaptic membrane by the transient reversals of the sign of membrane polarization that occur during action potential impulses in the postsynaptic cell. But, since the release of neurotransmitter drives the membrane potential of the synaptic zone towards a level about half-way between the negative-inside resting potential and the positive-inside action potential, it would follow that the membrane patches surrounding the receptors of a synapse whose activity has contributed to setting off the postsynaptic impulse would be spared the full extent of the noxious polarity reversal. This mechanism can account for a neurophysiologically documented example of the operation of Hebb's postulate, namely the plasticity of the connections between fourth- and fifth-order neurons in the visual cortex of cats.


Continuous propagation of radiation leukemia virus on a C57BL mouse-embryo fibroblast line, with attenuation of leukemogenic activity. The radiation leukemia virus (RadLV), a murine leukemia virus derived from thymic lymphomas induced by x-irradiation in strain C57BL/Ka mice, has been successfully propagated in sustained high titer in vitro in a newly established line, BL-5, of C57BL/Ka mouse-embryo fibroblasts. In addition, the production of endogenous virus, presumed to be RadLV, has been induced and sustained through multiple serial passages after treatment of BL-5 cell cultures with 5-bromodeoxyuridine. The chronically RadLV-infected subline, designated BL-5 (RadLV), sheds virus into the supernatant culture fluids that is biologically active in vitro in the XC cell plaque assay, in interference assays for focus-formation by murine sarcoma virus, and in the intracellular induction of group-specific antigens detectable by immunofluorescence, but is apparently devoid of leukemogenic activity after intrathymic inoculation into neonatal or immunosuppressed C57BL/Ka mice. Although BL-5 cells exhibited morphological alterations suggestive of transformation in vitro and gave rise to fibrosarcomatous ascites tumors after intraperitoneal inoculation with C57BL/Ka mice, the chronically infected BL-5(RadLV) cells remained normal in morphology and failed to yield fibrosarcomas in vivo.


Pituitary-adrenal responsiveness in surgical patients on acute and chronic corticosteroid therapy: plasma corticosterone assay.


On the fine structure of horse sweat glands.


The effect of aminophylline on pentagastrin-induced gastric secretion in the dog.


Sequential immunization procedure against Group B arboviruses using living attenuated 17D yellow fever virus, living attenuated Langat E5 virus, and living attenuated dengue 2 virus (New Guinea C isolate).


Axillary metastases from unknown primary sites.


Passive immunity for arbovirus infection. II. Quantitative aspects of naturally and artificially acquired protection in mice for Japanese (B) encephalitis virus.


Crystalloid inclusions in the Schwann cell cytoplasm.


The evaluation of a new peritoneoscope as a diagnostic aid to the surgeon.


Evidence that endogenous cyclic AMP does not modulate serum sulfation factor action on embryonic chicken cartilage.


Induction of serine dehydratase activity by cyclic AMP is restricted to S phase in synchronized CHO cells.


Amino acid compositions of simian virus 40 structural proteins.


Inhibition of Escherichia coli growth by cyclic adenosine 3', 5'-monophosphate.


Association of prolyl hydroxylase activity with membranes.


Adenylate cyclase activity is decreased in chick embryo fibroblasts transformed by wild type and temperature sensitive Schmidt-Ruppin Rous sarcoma virus.


Occurrence of cis-7-tetradecenoic acid in the envelope phospholipids of Escherichia coli K12.


Polyriboadenylate synthesizing activity in chromatin of wheat seedlings.


The inhibition of the avian myeloblastosis virus DNA polymerase by poly(U) fractions of varying chain length.


Identification and extent of fluid bilayer regions in membranous cytochrome oxidase.


A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system.


Localization of an RNA-degrading system of enzymes on the rat liver plasma membrane.


Space-time clustering of limb defects in Cardiff.


Settlement of Mr. Kenneth Robinson's libel action.


Infectious mononucleosis.

Infectious mononucleosis.


Cell-mediated immunity to Varicella-Zoster antigen in acute Herpes zoster (shingles).


Immunoconglutinin response in patients with acute viral respiratory disease.


Magnetic-circular-dichroism studies of cytochrome c and cytochrome b 2 .


Oxidation of acetaldehyde by rat-liver mitochondria in relation to ethanol oxidation and the transport of reducing equivalents across the mitochondrial membrane.


Molecular weight and subunit structure of the malate-lactate transhydrogenase.


Studies on metabolism of lung flukes of the genus Paragonimus. VI. Succinoxidase system in homogenates of eggs, larvae, and adults.


Plasma renin studies in patients with traumatic spinal cord injuries.


Limb-girdle muscular dystrophy. Case report with electron microscopic study of a muscle biopsy.


Growth and development of children with Legg-Calvé-Perthes syndrome in Israel.


The detection of Clostridium welchii in the differential reinforced clostridial medium technique.


Nitroblue tetrazolium reduction by neutrophils: biochemical and ultrastructural effects of methylene blue.


Studies on the mechanism of inflammation induced by calcium pyrophosphate crystals.


Intrathecal gentamicin in treatment of bacterial meningitis.


Dose-response to lead ingestion in rats fed low dietary calcium.


Twenty-four hour patterns of plasma luteinizing hormone and follicle-stimulating hormone in sexual precocity.


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 32-1973.


Clinical dermatology (third of three parts).


Lead inclusion bodies in osteoclasts.

Lead inclusion bodies in osteoclasts. Inclusion bodies occur frequently in the nuclei and rarely in the cytoplasm of osteoclasts in pigs with experimental lead poisoning. The light and electron microscope pictures of undemineralized sections are similar to those described for liver cord cells and renal tubular cells.


The prolactin cell of the white-crowned sparrow, Zonotrichia leucophrys pugetensis.


Ultrastructure of the aesthetase hairs of the littoral decapod, Paragrapsus gaimardii.


The fine structure of the lutein cells in the mink (Mustela vison) with special reference to the secretory activity during pregnancy.


The ultrastructural arrangement of thrombosthenin in glycerol extracted thrombocytes.


Progressive external ophthalmoplegia--some clinical associations.


An attempt to explain nervous transmitter release as due to nerve impulse-induced cation exchange.


Degeneration activity in the rat urinary bladder.


Afferent discharges via the laryngeal communicans related to respiration in the rat.


High-pressure anion exchange chromatography and enzymatic isotope displacement assays for cyclic AMP and cyclic GMP.


Saturation assay for cyclic AMP using endogenous binding protein.


Determination of relative levels of cyclic AMP in tissues or cells prelabeled with radioactive adenine.


An assay method for cyclic AMP and cyclic GMP based upon their abilities to activate cyclic AMP-dependent and cyclic GMP-dependent protein kinases.


Radioimmunoassay for the measurement of cyclic nucleotides.


The luminescence assay of cyclic AMP.

The luminescence assay of cyclic AMP.


Analysis of cyclic AMP and cyclic GMP by enzymic cycling procedures.


Firefly luminescence in the assay of cyclic AMP.


Protein binding assays for cyclic nucleotides.


Herpesvirus type 2 in genitourinary tract infections.


Effects of sonication on physicochemical and biological properties of synthetic double-stranded polyribonucleotides as interferon inducer.


Inhibitory effect of N-phenyl-N'-aryl or alkylthiourea derivatives on the multiplication of some picornaviruses (Coxsackie B 1, ECHO 19, and foot-and-mouth disease virus) in cell cultures.


Infection of chick embryo tracheal organ cultures with influenza A2 (Hong Kong) virus. II. Electron microscopy.


The use of frozen cells in the agar-cell suspension titration technique. A reproducible system. Brief report.


The ultrastructure of acute silicosis.

The ultrastructure of acute silicosis.


Bovine virus diarrhoea virus: acquired resistance to acriflavine. Brief report.


Morphological effects of Compound 48-80, polymyxin B sulfate, and dextran on rat skeletal muscle.


Possible role of cyclic AMP in gastric acid secretion in rat. Activation of carbonic anhydrase.


Multifocal granular cell myoblastoma. Report of a case involving trachea, stomach, and anterior abdominal wall.


The use of tetanic and single twitch stimuli to assess neuromuscular block in man.


The effects of oestrogen, ACTH and cortisone administration and hypophysectomy on histological changes induced by unilateral renal pedicle clamping.


Significance of high plasma renin activity in patients on maintenance haemodialysis therapy. Blood pressure and plasma renin activity were remeasured after an interval of 8 to 17 months in 16 patients on maintenance haemodialysis previously found to have high renin activity. Continuing intermittent haemodialysis changed the plasma renin activity and blood pressure but no consistent pattern was seen. This confirmed our previous finding that adequate dialysis can control blood pressure in the presence of high renin activity. We therefore conclude that bilateral nephrectomy should be approached with renewed caution.


Immunological assessment of mice with chronic jaundice and runting induced by reovirus 3.


Oestrogen receptor in human breast cancer tissue and response to endocrine therapy. Oestrogen receptor determinations were done in metastatic breast cancer tissue of patients with advanced breast cancer. In 37 patients with progressive disease evaluation of the response to endocrine treatment was possible, following the criteria of the E.O.R.T.C. Co-operative Breast Cancer Group. In 20 patients with receptor-negative tumours two objective remissions were noted; in 17 patients with receptor-positive tumours 14 objective remissions were seen. There seems to be a striking correlation between the presence or absence of oestrogen receptor in tumour tissue and the clinical response to hormonal therapy.


Radioimmunoassay for plasma renin activity.


A sensitive bioassay for adrenocorticotrophic hormone in human plasma.


Urinary pregnanediol and pregnanetriol in the salt-losing and nonsalt-losing forms of C-21-hydroxylase deficiency.


Cystosarcoma phyllodes.

Cystosarcoma phyllodes.


Effect of 1-naphthyl isothiocyanate and 3-methylcholanthrene on hepatocarcinogenesis in rats treated with diethylnitrosoamine.


Relations between postnatal days of mice and carcinogenic effect of cycasin.


Influence of vascular permeability on blood-borne metastasis.


Virus-like particles observed in transplantable intrahepatic bile duct carcinomas and hepatoma of Syrian golden hamsters.


Inhibitory effect of a streptococcal preparation (OK-432) on the nucleic acid synthesis in tumor cells in vitro.


Carcinoma of the large intestine of rats induced by rectal infusion of methylazoxymethanol.


A new method for the investigation of the kinetics of the capture reaction in phosphatase cytochemistry. I. Theoretical aspects of the local formation of crystalline precipitates.


What a dietitian should know about hyperlipidemia.


The vanishing chronic. The reduction of the resident patient population of a large urban Ontario mental hospital from 1950 to 1970 with an analysis of associated administrative and therapeutic changes.


A new method for the investigation of the kinetics of the capture reaction in phosphatase cytochemistry. II. Theoretical and experimental study of phosphate diffusion from thin polyacrylamide films.


Angiotensin II analogs. 10. Stereochemical factors in the 5 position influencing pressor activity.


Neutralizing activity in human sera against the leukocyte-transforming agent.


6-Substituted 5-chloro-1,3-dihydro-2H-imidazo(4,5-b)pyrazin-2-ones with hypotensive activity.


Antibiotics and the kidney.

Antibiotics and the kidney.


Viral cystitis.

Viral cystitis.


Further study on acute hemorrhagic cystitis due to adenovirus type 11.


Stomach contractions at REM sleep in goats and sheep.


Transplantability of avian lymphoid tumor cells.


Acetylcholine-dependent molecular receptor. Activation of triphosphoinositide phosphomonoesterase.


Oral tumours in Kenya.

Oral tumours in Kenya.


Normal structure, ultrastructure and enzyme content of glomic cells.


Roentgen and telecobalt therapy of tumors of the brain.


Electroneuroprostheses: past and present uses in man.


Ceramics, glasses, and composites in medicine.


Is there a vestibulo-spinal reflex contribution to running?


Cerebral hemodynamics in carotid surgery.


Experimental cerebellar uvulonodular lesions in the squirrel monkey.


The vestibular thalamus in the rhesus monkey.


Galvanic body sway in normals and patients with 8th nerve lesions.


Studies on methanol-oxidizing bacteria. I. Isolation and growth studies.


Long-acting phenothiazines in the prevention of relapse of schizophrenic patients.


Potentiation of rifampicin, rifampicin analogs, and tetracycline against animal cells by amphotericin B and polymyxin B.


DNA polymerases I and II in regenerating rat liver and Morris hepatomas.


A comparative study of some properties of chromatin from two "minimal deviation" hepatomas.


Viropexis of type B particles in reticulum cell sarcoma of R3-Dm strain mice.


Vincristine treatment of advanced cancer: a cooperative study of 392 cases.


Poly(adenosine diphosphate ribose) synthesis during the cell cycle of transformed hamster lung cells.


Separable DNA polymerase activities in host liver and Morris hepatomas.


Protein turnover in microsomal subfractions of liver and Morris hepatomas 7800 and 9618A.


Rapid arrest of DNA synthesis by N 6 ,O 2' -dibutyryl cyclic adenosine 3',5'-monophosphate in cultured hepatoma cells.


Protection against radiation leukemogenesis by repeated injections of immune and nonimmune foreign sera.


A selective review of experimental studies in cervical carcinoma.


A comparison review of key epidemiological studies in cervical cancer related to current searches for transmissible agents.


Latent infections induced by herpes simplex viruses.


Summary of informal discussion on general aspects of herpesviruses.


The transcription and state of herpes simplex virus DNA in productive infection and in human cervical cancer tissue.


Evidence for an oncogenic potential of the Epstein-Barr virus.


Simian herpesviruses.

Simian herpesviruses.


Oncogenicity of rabbit herpesvirus.

Oncogenicity of rabbit herpesvirus.


Oncogenic potential of guinea pig herpes- and C-type viruses.


Summary of informal discussion on oncogenicity of other herpesviruses.


Clinical features of herpes genitalis.

Clinical features of herpes genitalis.


Summary of informal discussion of part I of genital herpesvirus.


Relation of cytohistopathology of genital herpesvirus infection to cervical anaplasia. Cytological changes resulting from herpes simplex virus (HSV) were detected in 673 (.26%) of 279,589 cervicovaginal smears from an indigent hospital population.  Histopathological features of HSV infection were observed in 45 of 126 biopsies taken from the group of 673.  Most of the lesions were multifocal and were located on the squamocolumnar junction;  diagnostic cells were found at the base of normal squamous epithelium or at the surface of anaplastic epithelium.  The cytological and histological changes and diagnostic procedures for HSV infection are given in detail;  cytological detection is 2/3 as sensitive as viral isolation.  The presence of anaplasia in about 2/3 of the 673 HSV cases either before or at the same time as the detection of HSV suggests that anaplastic tissue is more susceptible to HSV infection.  However, serological evidence showing HSV antibodies before the onset of cervical anaplasia, the recurring nature of HSV, and epidemiological evidence showing the age of acquisition of HSV to be 5-30 years before diagnosis of cervical cancer are all compatible with the view that HSV causes cervical cancer.


A review of the identification and titration of antibodies to herpes simplex viruses type 1 and type 2 in human sera.


Possible etiologies of cancer of the cervix other than herpesvirus.


An analysis of seroepidemiological studies of herpesvirus type 2 and carcinoma of the cervix.


Prospective studies of the association of genital herpes simplex infection and cervical anaplasia.


Effect of herpesvirus type 2 and hormonal imbalance on the uterine cervix of the mouse.


Herpesvirus and cervical cancer studies in experimental animals.


Summary of informal discussion of part 3 of genital herpesvirus and of in vivo animal studies.


Human embryonic lung cells transformed by herpes simplex virus.


Transformation of hamster embryo fibroblasts by herpes simplex viruses type 1 and type 2.


Virions and antigens of herpes virus type 2 in cervical carcinoma.


Nucleic acid hybridization and the relationship between cervical cancer and herpes simplex virus type 2.


Summary of informal discussion of experimental approaches.


Symposium on herpesvirus and cervical cancer. Epilog.


Summary of papers delivered at the Conference on Herpesvirus and Cervical Cancer (Key Biscayne, Florida).


Epithelial cell surfaces and the basement membrane in the ocular lens, studied with the scanning electron microscope.


Cataracts induced by the polypeptide antibiotic polymyxin B sulfate.


Immunohistochemical demonstration of glucagon in an A 2 -cell carcinoma.


Distribution of vitamin D 3 : evidence of accumulation in renal proximal tubuli and thyroid parafollicular cells.


Effect of some drugs on the chemotaxis of rabbit neutrophils in vitro.


Effects of hypophysectomy on lactate metabolism in the perfused rat liver.


4-Uloses as intermediates in enzyme-nicotinamide adenine dinucleotide-mediated oxidoreductase mechanisms. I. Uridine diphosphate-galactose-4-epimerase.


4-Uloses as intermediates in enzyme-nicotinamide adenine dinucleotide-mediated oxidoreductase mechanisms. II. Regulation of enzymatic activity by reductive inhibition.


The pH dependence of the steady state kinetic parameters for staphylococcal nuclease-catalyzed hydrolysis of deoxythymidine-3'-phosphate-5'-p-nitrophenylphosphate in H 2  O and D 2  O.


Further studies of the interaction of cyclic adenosine 3':5'-monophosphate with endometrial protein kinase.


The vasopressin-sensitive adenylate cyclase of the rat renal medulla.


Studies on the kinetic mechanism of lipoamide dehydrogenase from rat liver mitochondria.


Assembly of the vesicular stomatitis virus envelope: incorporation of viral polypeptides into the host plasma membrane.


Transmural gradient of NAD-NADPH ratio in their canine left ventricular myocardium, and effects of coronary dilators on the transmural gradient.


Bone cell response to calcium active drugs.


The influence of psychiatry in medical education.


Streptozotocin-induced lactic acidosis. A case report and some experimental observations.


Methal-induced granulomatous hypersensitivity in man.


A low-temperature EPR study of partially purified, soluble ferric cytochromes P-450 and P-448 from rat liver microsomes.


The use of gel chromatography to study rapid equilibria of the type A + B forms and is formed from C + D.


Thermal transitions of proteins.

Thermal transitions of proteins.


Effects of preparation method on reversible conformational changes induced by neutral salts in spin-labeled erythrocyte membranes.


Variable response of renin activity in primary and secondary hypertension.


Proton relaxation rate and fluorometric studies of manganese and rare earth binding to concanavalin A.


Ultrastructure of rat odontoblasts in various stages of their development and maturation.


Observations on the ultrastructure of the buccal epithelium of the rat.


A comparison of kinetic parameters obtained with three major non-interconvertible isozymes of rat pyruvate kinase.


Partial purification and properties of rat liver mitochondrial polynucleotide phosphorylase. 1. Polynucleotide phosphorylase was partially purified from the inner membrane of rat liver mitochondria. 2. The partially purified particulate enzyme catalyses phosphorolysis of poly(A), poly(C), poly(U) and RNA to nucleoside diphosphates. 3. It is devoid of nucleoside diphosphate-polymerization activity. 4. Variable amounts of ADP/P(i)-exchange activity are associated with the polynucleotide phosphorylase and are probably due to a different enzyme. 5. ADP is the preferred substrate for exchange, and little or no reaction occurs with other nucleoside diphosphates, but ATP/P(i)-exchange takes place at one-third the rate observed with ADP. 6. The partially purified enzyme is free from the phosphatases found in the crude mitochondrial inner membrane, but is associated with an endonuclease activity and some adenylate kinase activity; no cytidylate kinase activity analogous to the latter was detectable.


Induction of bacterial enzymes.

Induction of bacterial enzymes.


Mechanism of action of cortisol in rat liver.


Preparation and some properties of a suspension of fragmented tubules from rat kidney. A method is described whereby short fragments of rat kidney tubule were obtained when kidney slices were gently dispersed by exposure to collagenase and hyaluronidase. When suspended in buffered saline the fragmented tubules respired actively over a period of several hours, the rate of oxygen consumption being proportional to the amount of cell protein. Oxygen uptake was stimulated by the addition of glucose, lactate, butyrate, alpha-oxoglutarate and other substrates and was decreased by the omission of Ca(2+) from the suspending medium. With alpha-oxoglutarate as the added substrate, dinitrophenol strongly stimulated oxygen uptake. Dinitrophenol had a less-marked stimulatory effect when glucose was the added substrate, and inhibited respiration in the absence of added substrate. Oligomycin inhibited respiration and this inhibition was partially reversed by dinitrophenol. Fragmented tubules synthesized glucose from lactate at a high rate but this capacity for gluconeogenesis was abolished by dinitrophenol and by physically damaging the cells.


Nitrogenase of Klebsiella pneumoniae: electron-paramagnetic-resonance studies on the catalytic mechanism.


Regulation of L-serine dehydratase in developing rat liver.


The induction of ornithine decarboxylase in cultured hepatoma cells.


Creatine kinase activity in cultures of differentiating myoblasts.


The effect of calcium on temperature-induced phase changes in liquid-crystalline cardiolipin structure.


Membrane reconstitution in chl-r mutants of Escherichia coli K 12. V. ATPase incorporation into particles formed by complementation.


B-cell amplification by dibutyryl cyclic AMP in mice.


Basic concepts underlying recent developments in the field of Parkinson's disease.


The relationship of rapidly labeled adrenal RNA synthesis to steroidogenesis in a superfusion system: effect of ACTH.


Acute effects of altered photoperiods on the onset of ovulation in gonadotropin-treated immature rats.


Endogenous gonadotropin requirements for follicular growth and maintenance in immature rats pretreated with PMS.


Inhibition of adrenocorticotropic hormone production by glucocorticoids in mouse pituitary tumor cells.


The effect of phenformin on lipolysis and the concentration of cyclic AMP and ATP in white fat cells.


Adrenal cells in tissue culture. X. On the mechanism of the stimulation of steroidogenesis by cyclic cytidine monophosphate.


Interaction of glucagon and catecholamines in the regulation of serine dehydratase.


Effects of p-chlorophenoxyisobutyrate (CPIB) on serum insulin levels, insulin sensitivity, and adipose tissue cyclic AMP concentration.


Control of insulin secretion by catecholamines, stress, and the sympathetic nervous system.


Insulin receptor of liver and fat cell membranes.


The problem of identifying the glucagon receptor.


Lipid peroxidation damage to cell components.


Control of free radical mechanisms in nucleic acid systems: studies in radioprotection and radiosensitization.


Falcon Herpesvirus, the etiologic agent of inclusion body disease of falcons. A viral agent has been isolated from five fatal cases of naturally occurring inclusion body disease in three different falcon species, namely, the prairie falcon (Falco mexicanus), the red-headed falcon (F. chiquera), and the peregrine falcon (F. peregrinus). The virus has been shown to possess the physical, chemical, and biological properties of a herpesvirus and has been used to reproduce inclusion body disease in the prairie falcon, merlin (F. columbarius), and American kestrel (F. sparverius). A similar disease was also produced with this virus in the great horned owl (Bubo virginianus), screech owl (Otus asio), and ring-necked turtle dove (Streptopelia risoria). Serological comparison of the falcon herpesvirus with other known avian herpesviruses revealed that the virus is antigenically closely related to a pigeon herpesvirus and an owl herpesvirus while differing from the former in host range. No antigenic relationship to infectious laryngotracheitis virus, duck virus enteritis, or Marek's disease virus could be demonstrated.


Surface antigens produced by Herpesviruses Varicella-Zoster virus. Surface antigen(s) was demonstrated by the mixed agglutination technique on cell cultures infected with varicella-zoster virus (V-Z). The reactions appeared to be specific for V-Z, and no cross-reaction was obtained with herpes simplex virus (HSV). V-Z surface antigen had similar physicochemical properties to that of HSV. It was stable to heating at 56 C and treatment with 1% Formalin, 2,4-dinitrophenol, and periodate, whereas it was undetectable after exposure to acetone or ethanol. Antibodies detecting surface antigen were present in high titer in sera from varicella or herpes zoster patients and paralleled titers obtained by the complement fixation test.


Hemagglutination-inhibition test in rhinovirus infections of volunteers. The hemagglutination-inhibition (HI) test for antirhinovirus antibody was carried out on paired sera from volunteers inoculated with rhinovirus type 3 or type 4 (RV4). The HI test gave results which paralleled the neutralization test and was at least as sensitive as a microneutralization method for detection of serotype-specific antibody. Although high levels of HI antibody in the serum were associated with protection from infection, in the case of RV4 low serum HI antibody levels did not necessarily imply susceptibility to challenge with small doses of virus. HI activity could be measured in concentrated nasal-washing fluids, and this antibody also seemed relevant to protection against infection.


immunoglobulin and histamine-sensitivity response of mice to live Bordetella pertussis. Mice injected intranasally (it.n.) and intraperitoneally (i.p.) with a nonlethal dose (2.5 x 10(5) colony-forming units) of live Bordetella pertussis were examined for 50 days for infection, respiratory tract immunoglobulins (Ig), changes in serum Ig, and histamine sensitivity. With mice infected it.n., respiratory infection markedly declined between day 20 and day 30. Ig classes (A, G(1), G(2a), G(2b), but no M), which had specificity for B. pertussis, were present in tracheobronchial wash (TBW) by day 15; by day 50, TBW immunodiffusion and immunoelectrophoretic precipitin bands were more intense. A sharp rise in serum IgA after day 30 was the only significant change relative to controls among the five serum Ig examined. A high degree of histamine sensitivity developed by day 15 to 20 and persisted for the 50 days. With mice inoculated i.p., no bacteria were recovered, no Ig or only traces were found in TBW and IgA only was specific, and no significant changes in the serum Ig relative to controls occurred. Histamine sensitivity developed somewhat more slowly and to a lesser degree than in it.n.-injected mice but persisted for the 50 days. A similar small number of killed bacteria (pertussis vaccine) injected it.n. or i.p. likewise induced slowly developing histamine sensitivity in contrast to published reports of 4 to 5 day peak sensitivity and decline following i.p. injection of 10(9) or more killed bacteria.


The clinical significance of pituitary-adrenal suppression by exogenous corticosteroids.


Hyperlipidemia in coronary heart disease. 3. Evaluation of lipoprotein phenotypes of 156 genetically defined survivors of myocardial infarction. Although analysis of lipoprotein phenotypes is widely used to diagnose and classify the familial hyperlipidemias, an evaluation of this system as a method for genetic classification has hitherto not been published. The present study of 156 genetically defined survivors of myocardial infarction was therefore designed to examine the relationship between lipoprotein phenotypes and genetic lipid disorders. The lipoprotein phenotypes of each survivor was determined primarily by measurement of his plasma triglyceride and low density lipoprotein (LDL)-cholesterol concentrations; his genetic disorder was identified by analysis of whole plasma cholesterol and triglyceride levels in relatives. The mean levels of LDL-cholesterol discriminated statistically among the three monogenic lipid disorders; it was highest in survivors with familial hypercholesterolemia (261+/-61 mg/100 ml [mean +/-SD]); intermediate in those with familial combined hyperlipidemia (197+/-50); and lowest in those with familial hypertriglyceridemia (155+/-36) (P < 0.005 among the three groups). However, on an individual basis no lipoprotein pattern proved to be specific for any particular genetic lipid disorder; conversely, no genetic disorder was specified by a single lipoprotein pattern. This lack of correlation occurred for the following reasons: (a) individual LDL-cholesterol levels frequently overlapped between disorders; (b) in many instances a small quantitative change in the level of either LDL-cholesterol or whole plasma triglyceride caused qualitative differences in lipoprotein phenotypes, especially in individuals with familial combined hyperlipidemia, who showed variable expression (types IIa, IIb, IV, or V); (c) lipoprotein phenotypes failed to distinguish among monogenic, polygenic, and sporadic forms of hyperlipidemia; (d) clofibrate treatment of some survivors with genetic forms of hyperlipidemia caused their levels of triglyceride and LDL-cholesterol to fall below the 95th percentile, thus resulting in a normal phenotype; and (e) beta-migrating very low density lipoproteins (beta-VLDL), previously considered a specific marker for the type III hyperlipidemic disorder, was identified in several survivors with different lipoprotein characteristics and familial lipid distributions. These studies indicate that lipoprotein phenotypes are not qualitative markers in the genetic sense but instead are quantitative parameters which may vary among different individuals with the same genetic lipid disorder. It would therefore seem likely that a genetic classification of the individual hyperlipidemic patient with coronary heart disease made from a quantitative analysis of lipid levels in his relatives may provide a more meaningful approach than determination of lipoprotein phenotypes.


Evidence for a common, saturable, triglyceride removal mechanism for chylomicrons and very low density lipoproteins in man. Hypertriglyceridemic subjects were fed diets in which dietary fat calories were held constant, but carbohydrate calories were varied. Three subjects with fasting chylomicronemia (Type V) were given less carbohydrate and four subjects without fasting chylomicronemia (Type IV) were fed diets with more calories as carbohydrate. The restricted carbohydrate intake led to disappearance of chylomicronemia in those subjects who had chylomicronemia on a normal diet (Type V to IV). In those subjects without chylomicronemia, chylomicronemia appeared in response to increased carbohydrate intake (Type IV to V). Thus chylomicron concentrations in plasma were altered even though fat intake and presumably chylomicron input into plasma was kept constant. These findings provide evidence for saturation of chylomicron removal mechanisms by alteration of endogenous triglyceride-rich lipoprotein concentrations. They suggest that chylomicrons compete with very low density lipoproteins for similar removal mechanisms. The relationship between endogenous triglyceride concentration and the lipolytic activity in plasma following heparin was then evaluated with the use of long-term heparin infusions to release and maintain lipolytic activity in the circulation. 10 subjects were placed on fatfree diets to remove circulating dietary fat. The plasma lipolytic rate during the heparin infusion was measured consecutively on different days in individuals whose triglyceride concentrations were varied by either increasing or decreasing calories. The lipolytic rate was curvilinearly related to the plasma triglyceride concentrations. This curvilinear relationship followed Michaelis-Menton saturation kinetics over a wide range of triglyceride concentrations on fat-free, high-carbohydrate diets, in multiple studies in a group of individuals. These studies suggest that endogenous and exogenous triglyceride compete for a common, saturable, plasma triglyceride removal system related to lipoprotein lipase.


Identification of inorganic pyrophosphate in human platelets and its release on stimulation with thrombin. Serum inorganic pyrophosphate (PPi) levels were consistently two- to threefold higher than plasma PPi prepared from the same blood. PPi was found in platelets in amounts ranging from 1.4 to 3 nmol/10(8) cells, using three different techniques for quantification. These levels are approximately 800 times higher than the mean PPi concentration in normal plasma and approximate the levels of ADP found in platelets by other workers. About 50% of platelet PPi was specifically released extracellularly after stimulation with thrombin. Timed release experiments showed a pattern of release that resembled that described for ADP and ATP. This pattern was clearly different from that shown by platelet calcium, serotonin, or beta-glucuronidase. Platelet inorganic pyrophosphatase was not released into the supernate in detectable amounts. Platelets from patients with nucleotide storage pool deficiency showed greatly reduced levels of PPi as compared with control. There was no detectable release of PPi into extracellular medium after thrombin addition to a suspension of these platelets.


Glucocorticoid-binding proteins in human acute lymphoblastic leukemic blast cells. The first known step in steroid hormone action is the association of the steroid with specific cytoplasmic steroid-binding proteins (SBP). Using a competitive binding assay, we detected, quantified, and partially characterized such a SBP in cytosol from glucocorticoid-sensitive human lymphoblastic leukemic blasts. The affinity of steroids for the SBP was directly related to their known killing potency. For example, steroids without glucocorticoid effect such as androstenedione, etiocholanolone, and tetrahydrocortisol were unable to displace radiolabeled dexamethasone from the SBP in the binding reaction. The dose-response curve for in vitro inhibition of [(3)H]thymidine uptake in leukemic blasts correlated closely with the binding affinity of glucocorticoids to the SBP, providing additional support for an essential physiologic role for SBP in steroid action. SBP activity was either greatly diminished or absent in glucocorticoid-resistant cells. Six patients who intially had SBP in their blasts and were responsive to combinations of drugs including glucocorticoids no longer had SBP activity detectable at a time when they no longer responded to combinations of drugs including glucocorticoids. In vitro [(3)H]thymidine uptake was not inhibited by steroids in leukemic blast cells lacking SBP activity. Other patients who had received some antileukemic therapy including glucocorticoids and who still had SBP in their leukemic blasts, were still responsive to drug combinations that included glucocorticoids. This appears to be the first study demonstrating glucocorticoid receptors in a human tissue.


Hemoglobin Abraham Lincoln, beta32 (B14) leucine leads to proline. An unstable variant producing severe hemolytic disease. An unstable hemoglobin variant was identified in a Negro woman with hemolytic anemia since infancy. A splenectomy had been performed when the patient was a child. The anemia was accompanied by erythrocyte inclusion bodies and excretion of darkly pigmented urine. Neither parent of the proposita demonstrated any hematologic abnormality, and it appeared that this hemoglobin variant arose as a new mutation. Erythrocyte survival in the patient was greatly reduced: the erythrocyte t(1/2) using radiochromium as a tag was 2.4 days, and a reticulocyte survival study performed after labeling the cells with L-[(14)C]leucine indicated a t(1/2) of 7.2 days. When stroma-free hemolysates were heated at 50 degrees C, 16-20% of the hemoglobin precipitated. The thermolability was prevented by the addition of hemin, carbon monoxide, or dithionite, suggesting an abnormality of heme binding. An increased rate of methemoglobin formation was also observed after incubation of erythrocytes at 37 degrees C. The abnormal hemoglobin could not be separated from hemoglobin A by electrophoresis or chromatography, but it was possible to isolate the variant beta-chain by precipitation with p-hydroxymercuribenzoate. Purification of the beta-chain by column chromatography followed by peptide mapping and amino acid analysis demonstrated a substitution of proline for beta32 leucine. It appears likely that a major effect of this substitution is a disruption of the normal orientation of the adjacent leucine residue at beta31 to impair heme stabilization.


Biologic and immunologic characterization and physical separation of ACTH and ACTH fragments in the ectopic ACTH syndrome. Extracts of tumors from 32 patients with the ectopic ACTH syndrome were subjected to simultaneous bioassay and radioimmunoassays for ACTH. Radioimmunoassays were performed using three antisera, one of which reacts with the extreme N-terminal 1-13 amino acid sequence of ACTH, the second with the N-terminal 1-23 sequence of the ACTH molecule, and the third with the C-terminal 25-39 amino acid sequence of ACTH. There was, in general, good correlation between bioactivity and N-terminal ACTH immunoreactivity. However, there were large excesses of both extreme N-terminal and C-terminal immunoreactive materials in most tumor extracts, which were not found in extracts of three human pituitaries. Three tumor extracts were subjected to molecular sieve chromatography on Sephadex G-50 fine resin. The bioactive ACTH eluted in the same fractions as pituitary ACTH (mol wt approximately 4,500 daltons) and reacted equally in all three ACTH radioimmunoassay systems. The bioactive tumor ACTH was neutralized by incubation with the C-terminal antiserum, indicating it has an intact C-terminal sequence of amino acids. The next several fractions from the Sephadex column contained a material, mol wt approximately 3,100, which was biologically inactive and had C-terminal immunoreactivity but no N-terminal or extreme N-terminal immunoreactivity. Incubation with the N-terminal 1-23 ACTH antiserum did not adsorb these C-terminal fragments, indicating they lacked an intact sequence of amino acids in this region. A smaller ACTH fragment (mol wt approximately 1,800 daltons) eluted in still later fractions and reacted with the extreme N-terminal antiserum but not with the N-terminal or C-terminal antisera. It had no steroidogenic activity, but appeared to have significant melanocyte-stimulating activity. It is concluded that, in addition to an ACTH similar, if not identical, to pituitary ACTH, tumors of patients with the ectopic ACTH syndrome contain both N-terminal and C-terminal ACTH fragments.


Cytomegalovirus infection. A case with meningoencephalitis.


Septicaemia due to Pasteurella pneumotropica. The literature concerning Pasteurella pneumotropica infection in animals and man is briefly reviewed and a case presented in which the organism was the cause of septicaemia in a patient receiving chemotherapy for myeloid leukaemia. Bacteriological findings are recorded and compared with those of other authors.


Evidence for complement activation via the alternate pathway in skin diseases, I. Herpes gestationis, systemic lupus erythematosus, and bullous pemphigoid. A patient with herpes gestationis, 6 of 6 patients with bullous pemphigoid, and 5 of 25 patients with systemic lupus erythematosus were found to have properdin deposited along the skin basement membrane.The patient with herpes gestationis demonstrated by immunofluorescence basement membrane deposition of C3 and C5 in the absence of C1q, immunoglobulins, and light chains. A second patient with herpes gestationis had C3 deposition with no demonstrable immunoglobulins or light chains. A thermolabile humoral factor(s) capable of depositing C3 (without C1q or C4) on normal skin basement membrane was found in the sera of both patients with herpes gestationis. No anti-basement membrane antibodies could be demonstrated in the sera of these patients.The patients with systemic lupus erythematosus and bullous pemphigoid who manifested properdin deposition also showed skin basement membrane deposits of C1q, C4, C3, C5, and immunoglobulins. C3 proactivator (C3PA) was also found deposited along the skin basement membrane of three patients with systemic lupus erythematosus and all six bullous pemphigoid patients. This study provides suggestive evidence that activation of complement is occurring via the alternate pathway in herpes gestationis. In systemic lupus erythematosus and bullous pemphigoid, both the classical (antibody) mediated activation of complement as well as the alternate pathway may be operative.


Malignant pulmonary neoplasms predisposing to spontaneous pneumothorax.


In vitro effect of human lymphotoxin on microorganisms.


Electron microscopic detection of simian-type virus particles in human milk.


The effect of intra-arterial contrast media on the small intestine of rabbits. An electron-microscopic study.


Biochemical and endocrinological studies in parasite-infested patients with and without infantilism.


Cyclic adenosine monphosphate, metabolites, and phosphorylase in neural tissue: a comparison a methods of fixation. Fixation of rat brain tissue by freeze-blowing, microwave irradiation, immersion of whole rats in liquid nitrogen, and decapitation into liquid nitrogen indicates that postmortem changes in metabolites and enzyme forms are minimal in freeze-blown brains. Cyclic adenosine monophosphate levels are lowest in microwave-irradiated brains, which has been interpreted by some investigators to indicate rapid fixation and minimal anoxia. However, the changes in phosphocreatine, adenosine triphosphate, lactate, and phosphorylase clearly demonstrate that fixation by freeze-blowing or immersion in liquid nitrogen more closely approximate the state in vivo.


Fungal genetics.

Fungal genetics.


On the mechanism of antiatherosclerotic agents: microchemical studies on the in vitro effects of pyridinol carbamate and estrogen (premarin) on phosphofructokinase and malate dehydrogenase in the arterial wall.


Psychophysiological parallels in dreams.


Synthesis and biological activity of some purine 5'-thio-5'-deoxynucleoside 3',5'-cyclic phosphorothioates.


Studies on the active-site sulfhydryyl groups of yeast alcohol dehydrogenase.


An interacting spin label study of the fluidizing and condensing effects of cholesterol on lecithin bilayers.


Optical analysis of electron micrographs of cytochrome oxidase membranes.


Studies on cell-coat macromolecules in normal and virus-transformed BHK 21-C13 cells.


REM sleep in four-month infants under home and laboratory conditions.


Effect of anionic surfactants, nonionic surfactants and neutral salts on the conformation of spin-labeled erythrocyte membrane proteins.


Characterization of hydrophobic regions in proteins by spin labeling technique.


The stoichiometry of the paramagnetic copper and the oxidation-reduction potentials of type I copper in human ceruloplasmin.


The effect of different fatty acid and sterol composition on the erythritol flux through the cell membrane of Acholeplasma laidlawii.


Purification of c-type cytochromes from Nitrosomonas europaea.


The purification and some properties of the molybdenum-iron protein of Chromatium nitrogenase.


Genital herpes simplex virus infection and gonorrhoea. Association and analogies.


Plasma renin activity, plasma angiotensin and plasma and urinary electrolytes in normal and toxaemic pregnancy, including a prospective study.


Vasopressor-induced natriuresis and altered intrarenal haemodynamics in cirrhotic man.


The measurement of arginine vasopressin in human plasma by radioimmunoassay.


Adipose tissue cellularity and metabolism in newly diagnosed juvenile diabetics.


Interaction of tellurite with the respiratory chain in rat liver mitochondria.


Size of the RNA's synthesized by purified calf thymus DNA-dependent RNA polymerases on SV40 DNA.


A study of tRNA methylase action.

A study of tRNA methylase action.


The oxidation of acetate by liver mitochondria.


Specific effect of estrogens on an interaction between the uterine estradiol receptor and DNA.


Purification of virus glycoproteins by affinity chromatography using Lens culinaris phytohaemagglutinin.


Submitochondrial localization of DNA polymerase in rat liver tissue.


Effects of manganese on the activity of glycolytic and gluconeogenic enzymes in the perfused rat liver.


The significance of enzyme histochemical patterns in carcinomas of the large intestine in man. The activities of 13 enzymes in 40 carcinomas of the large bowel have been studied using histochemical techniques. Five enzymes-non-specific esterase, monoamine oxidase, succinate dehydrogenase, cytochrome oxidase, and acid phosphatase-commonly show much less activity in the tumours than in adjacent normal colon. The tumours have been classified based upon the number of enzymes which show marked reduction in activity in each tumour (types 1-5). The enzyme histochemical type and the size of the tumours have been jointly correlated with the presence of lymph node metastasis. Small type 1 or 2 tumours do not appear to be associated with metastatic disease. Small type 5 tumours were commonly associated with secondary carcinoma in the lymph nodes. Large tumours (greater than 25 sq cm surface area) of any histochemical type were frequently associated with lymph node metastasis. Discussion of the possible reasons for these findings and their clinical significance is presented.


E.P.R. of free radicals formed in DNA and its constituents after exposure to discharge-excited inert gases. II. Purines and DNA.


Dysphagia, vomiting and obdurate constipation as a metabolic manifestation of malignancy.


Water-soluble and pentanol-extractable proteins in human brain normal tissue and human brain tumours, with special reference to S-100 protein.


Myelin subfractions in developing rat brain: characterization and sulphatide metabolism.


The effect of ionizing radiation upon mitochondria of the central nervous system.


Mitochondrial cytochrome oxidase and monoamine oxidase activities in the developing chick cerebral hemispheres.


The occurrence of a glycine cleavage system in mammalian brain.


Availability to neuraminidase of gangliosides and sialoglycoproteins in neuronal membranes.


Electron microscopic identification of papova virus in laryngeal papilloma.


Bicuculline, GABA and central inhibition.


Infantile polymyoclonia.

Infantile polymyoclonia.


Hyperkalaemic periodic paralysis associated with Addison's disease.


Dysaesthesia-dyskinesia: a syndrome of painful legs and moving toes.


Spontaneous regression of leukemia in chickens infected with avian myeloblastosis virus.


Isolated pituitary cells: CRF-like activity of neurohypophysial and related polypeptides.


Calcification of a lysozyme-inositol phosphatide complex in vitro.


Suppression of established Friend virus leukemia by statolon. VII. Relative roles of interferon and the immune response in development of FV-dormant infections.


The effect of riboflavin deficiency on key gluconeogenic enzyme activities in rat liver.


Enhanced growth of a polyoma transformed tumor cell in Spirometra mansonoides-infected hamsters.


Further studies of viral antibodies in the cerebrospinal fluid of patients with multiple sclerosis: vaccinia and parainfluenza type 1.


Propagation of Rauscher leukemia virus to human lymphoblastoid cells by in vivo diffusion chamber cultivation.


The effect of LATS and TSH on protein and RNA synthesis in isolated thyroid cells.


Body movements during sleep after sleep loss.


DNA polymerases in RNA tumor viruses and possible "protoviruses".


Trans-sphenoidal surgery for pituitary lesions.


The presence and expression of RNA tumor virus genes in normal and infected cells: detection by molecular hybridization.


Introductory review. Molecular mechanisms in viral neoplasia of animals and their implications in the origin of cancer in man.


Evidence for 30-40S RNA as precursor of the 60-70S RNA of Rous sarcoma virus.


Evidence for viral involvement in murine and human mammary adenocarcinoma.


Molecular evidence for a viral etiology of human leukemias, lymphomas, and sarcomas.


Animal model of human disease: primary hepatocellular carcinoma.


Molecular probes in studies of the relationship of viruses to human neoplasia.


Interaction of angiotensin with disseminated intravascular coagulation: a possible mechanism in the genesis of acute renal failure. Because of the importance of the renin-angiotensin system in renal homeostatic mechanisms, the effect of angiotensin administration upon disseminated intravascular coagulation has been studied in rabbits. An infusion of angiotensin II (0.1 mug/kg/min for 2 hours) produced neither histologic abnormalities in the kidneys nor an elevation of creatinine. After an infusion of thrombin (2.0 units/kg/min for 2 hours) only 3 of 10 rabbits, when sacrified 24 hours later, showed histologic lesions comprised of occasional fibrin thrombi and foci of tubular necrosis. Creatinine levels did not rise. In contrast, the combination of angiotensin and thrombin resulted in renal lesions in 12 of 14 animals. Four had frank cortical necrosis, while combinations of tubular necrosis, glomerular thrombosis and segmental glomerular infarction occurred in the others, together with elevated creatinine levels. Blockade of alpha-adrenoreceptors with phenoxybenzamine in 12 animals did not prevent either these histologic changes or creatinine elevation, showing that the effect of angiotensin was independent of alpha-adrenoreceptor stimulation. The synergistic interaction between angiotensin and disseminated intravascular coagulation was not explained by differences in the consumption of plasma fibrinogen but apparently was due to localization of fibrin thrombi within glomerular capillaries by the vasomotor actions of angiotensin, as has previously been shown to occur with alpha-adrenoreceptor simulation. Such a mechanism might contribute to renal glomerular deposition of fibrin in acute ischemic renal failure.


Gastrontestinal cytomegalovirus infection.


Duodenal wall tumors and the Zollinger-Ellison syndrome. Surgical management.


A soluble CO-binding c-type cytochrome from the marine bacterium Beneckea natriegens.


Biochemical and biophysical studies on cytochrome c oxidase. X. Spectral and potentiometric properties of the hemes and coppers.


Carotid body chemodectomas: recent experience at University of Alabama hospitals.


Inhibition of 5 -reductase activity of rat prostate by estradiol derivatives.


The pneumonocytes in the lung of Xenopus laevis.


Measurement of inorganic pyrophosphate in biological fluids. Elevated levels in some patients with osteoarthritis, pseudogout, acromegaly, and uremia. A rapid and relatively simple method for measurement of inorganic pyrophosphate (PPi) in biological samples has been described. The mean +/-SEM of plasma samples from 94 normal subjects was 1.8+/-0.06 muM, giving a normal range (99% confidence limits) of 0.16 - 3.40 mumol/liter. Analysis of 17 plasma samples in duplicate showed a standard deviation of 0.18, giving a 99% probability that a single determination of plasma PPi would be +/-0.68 muM of the true value. The mean PPi levels in plasma from subjects with osteoarthritis, pseudogout, acromegaly, and uremia were significantly greater than the normal mean (P < 0.01). Samples from rheumatoid arthritis showed PPi levels distributed about a mean identical to the normal mean. Plasma inorganic orthophosphate levels correlated positively with PPi levels in samples from normal subjects and in samples from patients with osteoarthritis, pseudogout, and uremia, but not with acromegaly. This correlation was statistically significant only in the normal samples and in those from patients with osteoarthritis.


Simulation of tissue properties in irreversible diffuse obstructive pulmonary syndromes. Enzyme digestion. The length-tension properties of alveolar wall from normal cats were studied before and after exposure to enzymes naturally found in mammals (elastase, trypsin, collagenase, hyaluronidase). Hyaluronidase effected little change while all the proteolytic enzymes altered the mechanical properties of lung tissue. Collagenase removed the "mechanical stop" and the alveolar walls fractured at low forces. The properties of wall exposed to trypsin resembled those of elastase-treated tissue. Elastase increased the extension necessary to reach a given force and increased the maximum length (L(max)) and resting length (L(o)). Maximum extensibility (lambda(max)), the ratio of L(max) to L(o), fell with both elastase and trypsin digestion. A reduction in lambda(max) simulates the changes in alveolar wall properties seen in the lungs of the aged and in those with an irreversible diffuse obstructive pulmonary syndrome (DOPS(I)). Unlike these states, however, the energy loss in stretching alveolar wall increased with elastolysis. Furthermore, the changes in L(o) necessary to effect a change in lambda(max) of alveolar wall comparable to that seen in DOPS(I) were excessive. The altered tissue properties that occur in man with obstructive pulmonary syndromes could not be produced with these proteolytic enzymes or with hyaluronidase.