Biosynthesis of geraniol and nerol and beta-D-glucosides in Pelargonium graveolens and Rosa dilecta. 1. 3R-[2-(14)C]Mevalonate was incorporated into geranyl and neryl beta-d-glucosides in petals of Rosa dilecta in up to 10.6% yield, and the terpenoid part was specifically and equivalently labelled in the moieties derived from isopentenyl pyrophosphate and 3,3-dimethylallyl pyrophosphate. A similar labelling pattern, with incorporations of 0.06-0.1% was found for geraniol or nerol formed in leaves of Pelargonium graveolens The former results provide the best available evidence for the mevalonoid route to regular monoterpenes in higher plants. 2. Incorporation studies with 3RS-[2-(14)C,(4R)-4-(3)H(1)]-mevalonate and its (4S)-isomer showed that the pro-4R hydrogen atom of the precursor was retained and the pro-4S hydrogen atom was eliminated in both alcohols and both glucosides. These results suggest that the correlation of retention of the pro-4S hydrogen atom of mevalonate with formation of a cis-substituted double bond, such as has been found in certain higher terpenoids, does not apply to the biosynthesis of monoterpenes. It is proposed that either nerol is derived from isomerization of geraniol or the two alcohols are directly formed by different prenyltransferases. Possible mechanisms for these processes are discussed. 3. The experiments with [(14)C,(3)H]mevalonate also show that in these higher plants, as has been previously found in animal tissue and yeast, the pro-4S hydrogen atom of mevalonate was lost in the conversion of isopentenyl pyrophosphate into 3,3-dimethylallyl pyrophosphate.


Isolation and characterization of membranes from normal and transformed tissue-culture cells. Homogenates of baby-hamster kidney cells and rat embryo fibroblasts prepared by nitrogen cavitation contain a small population of slowly sedimenting mitochondria or mitochondrial fragments, which contaminate the microsomal fraction. This appears to limit the resolution of surface membrane and endoplasmic reticulum on magnesium-containing dextran gradients. The microsomal material and mitochondria can, however, be completely separated on a 10-60% (w/w) sucrose zonal gradient containing a 30% sucrose plateau. On magnesium-containing dextran gradients this mitochondria-free microsomal material can be resolved into at least two surface membrane fractions and at least two endoplasmic reticulum fractions. Comparison of polyoma virus-transformed and normal baby-hamster kidney cells reveals some interesting differences in their microsomal fractionation patterns and the characteristics of the Na(+)/K(+)-Mg(2+) adenosine triphosphatase of their surface membranes, in particular a tenfold lower K(m) in the virus-transformed cells. The fractionation patterns of normal and spontaneously transformed rat embryo fibroblasts are also briefly discussed, particularly in relation to the significance of the observation that both the surface membrane and endoplasmic reticulum from these cells can be subfractionated.


The regulation of rabbit liver fructose 1,6-diphosphatase activity by phospholipids in vitro.


Evidence for modification of protein phosphorylation by cytokinins. Kinetin stimulated phosphorylation of protein in floated Chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from Chinese-cabbage or tobacco leaves. Kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. Purified Chinese-cabbage leaf ribosomes exhibited protein kinase activity which was inhibited by kinetin and zeatin. The ribosome-associated kinase responded to kinetin and zeatin differently from that associated with nuclei+chloroplast preparations. Protein phosphorylation in vitro was not affected by adenosine 3':5'-cyclic monophosphate, indol-3-ylacetic acid or gibberellic acid. It was only inhibited by N(9)-unsubstituted purines, among which the known cytokinins were the most effective inhibitors. The results are discussed in relation to possible similarities between the effects of cytokinins in plant tissues and the effects of adenosine 3':5'-cyclic monophosphate in animal tissues. Both compounds appear to modify the activity of protein kinases and both affect many different cellular processes.


Isolation and characterization of lipoprotein B from high-density human serum lipoproteins. 1. Lipoprotein B from female Lp(a)-lipoprotein-negative serum was isolated from the fraction of density 1.073-1.125 by using immunoadsorbent; 2.5mg of freeze-dried material was obtained from 100ml of serum from a fasting patient. 2. The hydrated density of this lipoprotein was found to be 1.084g/cm(3). A flotation rate F(1.200) of 9.4 and lipid/protein ratio 1.40 were found, similar to that of high-density (d 1.073-1.125) lipoprotein preparations. 3. From immunochemical and electrophoretic studies of the intact and totally delipidized lipoprotein B it was concluded that this lipoprotein represents a separate family within the high-density range of human serum lipoproteins. 4. The possibility that the isolated lipoprotein B is an artifact created by the isolation procedure is discussed.


Can Culex pipiens fatigans or Aedes aegypti spread smallpox? A preliminary report.


Development of the activities of enzymes of the isoprenoid pathway during early stages of pea-seed germination. The activities of individual enzymes of the isoprenoid pathway from mevalonate kinase to squalene synthetase in homogenates of seeds germinated up to 32h were assayed. Changes in the activity of each enzyme were observed and compared with the activity at the 2h germination stage. Activities of alkaline phosphatase and fructose 1,6-diphosphate aldolase were similarly measured to provide a reference for changes in the general metabolic activity of seeds during imbibition of water. Water uptake reached a plateau after 12h. The reference enzymes almost doubled in activity between 2 and 8h and thereafter their activities steadily declined. All of the enzymes of the isoprenoid pathway increased in activity between 2 and 6h and, thereafter, with the exception of the prenyltransferase, their activities remained relatively constant. With the prenyltransferase activity the initial increase was followed by a short plateau between 6 and 9h and then a second increase to a maximum between 14 and 16h. After 16h the activity declined. The relative activities of the isoprenoid enzymes at 16h of germination were mevalonate kinase>phosphomevalonate kinase>pyrophosphomevalonate decarboxylase approximately isopentenyl pyrophosphate isomerase>squalene synthetase>isopentenyl pyrophosphate/dimethylallyl pyrophosphate prenyltransferase. The finding that the prenyltransferase may be the rate-limiting enzyme in squalene synthesis from mevalonate is discussed in relation to regulation of isoprenoid synthesis during pea-seed germination.


Proteolytic activity of Entamoeba histolytica. 1. Detection of enzyme by photographic film band.


The identification of the enzymes that catalyse the oxidation of glyoxylate to oxalate in the 100000 g supernatant fraction of human hyperoxaluric and control liver and heart tissue.


Electron microscopic evidence of the subviral oncogenic particles (virosomes) isolated from the mitochondria of Rous sarcoma cells.


Classroom approach teaches patients independence skills.


Hospital takes group therapy to released alcoholics in distant community.


A drug unit for life-style change: the Tellurian community.


The production of vesicular stomatitis virus by antigen- or mitogen-stimulated lymphocytes and continuous lymphoblastoid lines. A variety of lymphoid cell populations were examined in terms of their ability to replicate vesicular stomatitis virus (VSV), a lytic, RNA-containing virus maturing at the cell surface. The number of cells capable of producing VSV was estimated in terms of infectious centers by the virus plaque assay (VPA), and morphologically by electron microscopy (EM). The lymphoid cells examined in this study included: (a) lymph node cells from delayed hypersensitive guinea pigs stimulated by specific antigen, (b) mouse spleen cells activated by selective bone marrow-derived (B) cell and thymus derived (T) cell mitogens, and (c) cells of human and murine continuous lymphoblastoid or lymphoma lines. In unstimulated cultures of guinea pig lymph node cells there is a background of approximately 1 in 1,000 cells which produces VSV; in purified protein derivative (PPD)-stimulated cultures the number of cells producing virus was 1.6% in the VPA and 1.9% by EM. These cells were large lymphocytes with some morphological features of transformed lymphocytes but were not typical blast cells. A few macrophages were associated with virus in both stimulated and control cultures. These observations indicate that (a) cells responsive to antigens, as detected by a marker virus, were lymphocytes; (b) cells other than lymphocytes (macrophages) were capable of replicating VSV even without antigenic stimulation; and (c) the correlation of results obtained by VPA and morphologic examination was usually quite good. Of the total number of mouse spleen cells stimulated with concanavalin (Con A), a T cell mitogen, 4.5 (EM)-5.7% (VPA) were associated with VSV. These were characteristic transformed lymphocytes, similar to phytohemagglutinin (PHA)-stimulated human lymphocytes. In contrast Escherichia coli lipopolysaccharide (LPS)-treated mouse spleen cultures contained lower numbers of virus plaque-forming cells. The majority of such cells associated with virus displayed extensive rough endoplasmic reticulum. Two cultured murine lymphomas containing lymphocytes with the theta surface marker (L5178Y and EL-4) showed a 15-100-fold higher incidence of virus-producing cells than leukemias (L1210 and C57Bl/6) which did not carry this marker. Similarly, the L2C guinea pig leukemia, a known B cell leukemia, yielded a low percent of virus plaque-forming cells (<2%). However, MOPC-104, a plasma cell tumor presumed to be of B cell origin, was found to be an efficient virus producer. There was a wide variation in the efficiency of VSV replication among human lymphoblastoid lines. One line, Wil-2, produced 80% infectious centers after 24 h of exposure to VSV, and all cells were associated with virus at the EM level. The relationship between the virus-producing cells and different lymphocyte subpopulations as well as the efficiency of the two assays for studying virus-producing lymphocytes is discussed.


Induction of arginase activity with the Shope papilloma virus in tissue culture cells from an argininemic patient. Inoculation of the Shope virus in tissue cultures of human fibroblasts from a patient with a deficiency of the enzyme arginase results in an induction of arginase activity, apparently virus coded.


The effect of Bordetella pertussis on lymphocyte cyclic AMP metabolism. Bordetella pertussis culture fractions produce decreased metabolic responses to isoproterenol and epinephrine in mice and rats, suggesting the possibility of systemic beta adrenergic blockade. The present study was undertaken to elucidate the mechanism of the alteration in adrenergic responsiveness and to clarify its relationship to other biological effects of the organism. Lymphocytes were selected as a suitable tissue because of the marked alteration in lymphocyte distribution in pertussis-treated mice and rats, suggesting a change in the surface properties of these cells. Human peripheral blood lymphocytes, purified by nylon fiber chromatography, were studied. In short incubation experiments (20 min or less) B. pertussis did not alter the cyclic AMP response to isoproterenol, prostaglandin E (PGE(1)), or methacholine. However, when cells were preincubated with B. pertussis for 90 min at 37 degrees C, the responses to all three agents were markedly inhibited. Although these observations provide direct confirmation of the ability of B. pertussis to inhibit catecholamine responsiveness, the fact that PGE(1) and methacholine responses were also inhibited suggests that blockade at the level of the beta adrenergic receptor is doubtful. The inhibitory activity was localized in a nondialyzable, protein-rich fraction that is precipitated from B. pertussis culture fluid by ammonium sulfate at 90% of saturation. The bulk of the activity was obtained in the load volume after 50,000 g centrifugation in a cesium chloride gradient, density 1.2-1.5 (fraction 4). Fraction 4 produced a change in lymphocyte hormonal responsiveness at concentrations as low as 5 ng/ml. The relationship between cyclic AMP inhibitory activity in isolated human cells and leukocytosis-producing activity in intact mice was studied. The two activities seemed to parallel one another quite closely until the final Sephadex G-150 fractionation step, in which the two activities were obtained in the same column fraction, but a greater recovery of the leukocytosis-producing activity was obtained. Additional purification will be required to establish conclusively whether the same macromolecule is responsible for both activities. The availability of a bacterial product that markedly inhibits cyclic AMP accumulation in purified lymphocytes may help to clarify the role of cyclic AMP in lymphocyte activation by antigen and nonspecific mitogens.


Relation of chromosome 4 (linkage group 8) to murine leukemia virus-associated antigens of AKR mice. Genes specifying or controlling the expression of G(IX) (cell surface), GCSA (cell surface), and gs (internal viral) antigens are located in chromosome 4 (linkage group [LG] VIII) of the AKR mouse. All three antigens may exhibit mendelian inheritance, mice being antigen positive or antigen negative, but each may also appear in leukemic cells of mice whose inherited genotype was antigen negative. The G(IX)-determining gene in LG VIII of AKR mice apparently is equivalent to Gv-1, which determines expression of the same antigen in 129 strain mice, but which in the latter strain is located in LG IX. As the estimated distance of Gv-1 from H-2 in 129 mice is considerable (37 units) further tests are now indicated to assess the possibility of pseudolinkage in this case. The Fv-1 locus, also located in LG VIII, influences the mouse's titer of MuLV, and might thereby be thought to regulate the G(IX) and gs phenotypes of AKR backcross segregants. But the data indicate a discrete LG VIII locus for G(IX), since expression of this antigen is mendelian and independent of infectious virus titer. Since the G(IX) and GCSA phenotypes of AKR backcross segregants were invariably concordant, these two antigens must be specified or controlled by closely linked genes, and the latter also is presumably independent of virus titer. The question as to what extent expression of gs antigen in the segregants is secondary to virus production is undecided.


Radiographic and physiologic patterns among workers engaged in manufacture of asbestos cement products: a preliminary report.


Antagonism of peripheral and central effects of angiotensin II by (1-sarcosine, 8-isoleucine)angiotensin II.


Metabolic factors involved in the interaction between pyrazole and butane-1,4-diol and 4-hydroxybutyric acid.


Inhibition of lysosomal enzyme release by adenosine 5' triphosphate and N 6 ,O 2 -dibutyryl cyclic 3',5'-adenosine monophosphate.


Effects of denervation and lack of calcium on the action of Latrodectus venom on rat sympathetic ganglion.


Effect of mineralocorticoid agonists and antagonists on binding of  3 H-aldosterone to adrenalectomized rat kidney plasma membranes.


The inhibitory effects of theophylline feeding on gluconeogenic enzymes in rat liver.


Stimulation of mitochondrial DNA synthesis in HeLa cells by Herpes simplex virus.


Cyclic-AMP binding sites on the cell surface of thymic lymphocytes.


Isosmotic intracellular regulation and redox changes in the respiratory chain components of Callinectes sapidus isolated muscle fibers.


Aortic proline hydroxylase in hypoxia induced arteriosclerosis in rabbits.


Hepatic surgery.

Hepatic surgery.


Increased phosphofructokinase concentration in hepatoma 3924-A: enzymic and immunological evidence.


Association of cervical cytomegaloviruses with venereal disease.


Hepatocyte specificity for  -foetoprotein production.


A study on the levels of nucleic acids, protein and phosphatases in normal and X-irradiated guinea pigs.


Interactions of components of human rhinovirus type 2 with Hela cells.


Ribonucleases of Rous sarcoma virus.

Ribonucleases of Rous sarcoma virus.


Simian virus 40 DNA integration within the genome of virus-transformed mammalian cells.


The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy.


An analysis of the physical and chemical factors involved in the reactivation of neutralized poliovirus by the method of freezing and thawing.


Host cell DNA synthesis as a possible factor in the enhancement of replication of human adenoviruses in simian cells by SV40.


Adenosine 3',5'-cyclic monophosphate regulation of chloramphenicol acetyltransferase synthesis in vitro from P1CM DNA.


Polyacrylamide gel electrophoresis of fowlpox and vaccinia virus proteins.


Studies of SV40 DNA. VI. Cleavage of SV40 DNA by restriction endonuclease from Hemophilus parainfluenzae.


Morphological aspects of the hypothalamic-hypophyseal system. IV. Medial basal hypothalamus. An experimental morphological study.


Profound cystic colitis. Colitis cystica profunda.


Ultrastructural aspects of the nucleus infundibularis dorsalis in the caudal hypothalamus of Xenopus laevis.


Adenosine 3',5'--cyclic monophosphate dependent protein kinase and phosphoprotein phosphatase activities in rat islets of Langerhans.


The preparation of a modified GTP-sepharose derivative and its use in the purification of dihydroneopterin triphosphate synthetase, the first enzyme in folate biosynthesis.


Effect of histamine of lipolysis and adenosine 3',5'-monophosphate levels in canine adipose tissue.


The mechanism of the nicotinamide adenine dinucleotide stimulation of lipid phosphorylation in a rat liver microsome-cytosol system.


Cell density dependent glycolipids in NILz hamster cells, derived malignant and transformed cell lines.


Effect of phospholipids on lipoprotein lipase activation in vitro.


Lipid composition, Ca 2+  uptake and Ca 2+ -stimulated ATPase activity of sarcoplasmic reticulum of the cardiomyopathic hamster.


Production of cholinesterase by Pseudomonas aeruginosa, its regulation by glucose and cyclic AMP and inhibition by antiserum.


Corticosteroid binding macromolecules in the nucleus and cytosol of rat thymus cells.


Endogenous production of steroids by subcellular fractions from total rat testis and from isolated interstitial tissue and seminiferous tubules.


Involvement of thiol enzymes in the lysosomal breakdown of native and denatured proteins.


Phospholipids in artificially induced muscular dystrophy of calves.


Submandibular duct lithiasis associated with a mixed salivary gland tumor. A case report.


Proteinaceous precipitate in nodular (follicular) lymphomas.


Correlation of suspicious mammograms and x-rays of breast biopsies during surgery. Results in 60 cases.


Carcinoma of the kidney in a 15-year-old boy. Unusual histologic features with formation of microvilli.


Primary thymic seminoma--a neoplasm ultrastructurally similar to testicular seminoma and distinct from epithelial thymoma.


Sclerosing stromal tumors of the ovary.

Sclerosing stromal tumors of the ovary.


Ultrastructure of the renal arterial bifurcation of rabbits.


Isolation of oncornaviruses from chemically induced murine leukemias.


The intracellular site of surfactant synthesis. Autoradiographic studies on murine and avian lung explants.


NADH-cytochrome C reductase activity of antigen-stimulated macrophages.


Enzyme activity in the fluid of the human ovarian follicle.


Cinemicrography of pyrene perchlorate crystal formation by electrolysis.


Renal sodium- and potassium-activated adenosine triphosphatase and sodium reabsorption in the hypothyroid rat. The relationship between net tubular reabsorption of sodium and renal microsomal sodium- and potassium-activated adenosine triphosphatase (Na-K-ATPase) was evaluated in hypothyroid and hyperthyroid rats and in age-matched euthyroid controls. Tubular sodium reabsorption per gram of kidney was lower in thyroidectomized rats than in controls (186+/-14 vs. 246+/-12 mueq/min; P < 0.005) and was accompanied by a quantitatively similar reduction in Na-K-ATPase specific activity (49.4+/-2.4 vs. 65.8+/-2.3 mumol inorganic phosphate (P(t))/mg protein per h; P < 0.001). This decrement was present in both cortex and outer medulla, and was limited to Na-K-ATPase since other representative enzymes not involved in sodium transport (magnesium-dependent adenosine triphosphatase [Mg-ATPase], glucose-6-phosphatase, 5'-nucleotidase) remained unchanged or increased in the hypothyroid animals. Conversely, Na-K-ATPase rose when sodium reabsorption increased in euthyroid rats treated with triiodothyronine. Subsequent experiments were performed to determine to what extent the decrease in Na-K-ATPase is due to lack of thyroid hormone per se or to an adaptive response to decreased reabsorptive sodium load. Triiodothyronine in concentrations of 10(-12) to 10(-5) M had no effect in vitro on microsomal Na-K-ATPase of either thyroidectomized or euthyroid rats. When hypothyroid rats were uninephrectomized or treated with methylprednisolone, sodium reabsorption per gram kidney increased markedly and was similar to that of intact controls. Despite persistence of the hypothyroid state, Na-K-ATPase specific activity also increased to levels not significantly different from euthyroid animals. These data suggest that decreased tubular sodium transport is a major determinant of the reduction in renal Na-K-ATPase in thyroid deficiency since the latter can be reversed by increasing sodium reabsorption during continuing hypothyroidism. Furthermore, the modest sodium leak of hypothyroid animals does not appear to be due to decreased Na-K-ATPase since it was not corrected by uninephrectomy despite restoration of both cortical and medullary Na-K-ATPase activity to normal by this maneuver. The close correlation between net sodium reabsorption and Na-K-ATPase in all the experimental situations described here demonstrates that renal Na-K-ATPase changes adaptively in hyper- or hypothyroidism as it does in numerous situations in the normal animal, in accord with its postulated role in the active transport of sodium across the renal tubule.


Comparative effects of angiotensin and ACTH on cyclic AMP and steroidogenesis in isolated bovine adrenal cells. The comparative effects of angiotensin II and adrenocorticotropic hormone (ACTH) on cyclic AMP and steroidogenesis were investigated employing isolated bovine adrenal cells from the zona fasciculata. Like ACTH, angiotensin produced a prompt increase in cyclic AMP which preceded the increase in corticosteroid production. Although this increase in cyclic AMP was small when compared to that induced by ACTH, it correlated with the amount of steroidogenesis. This observation is consistent with the view that cyclic AMP is the intracellular mediator of the steroidogenic action of angiotensin. Angiotensin acted synergistically with ACTH on cyclic AMP levels. This synergism was not explained by inhibition of phosphodiesterase activity. Unlike ACTH, angiotensin failed to stimulate adenylate cyclase in broken cell preparations. The observations suggest that more than one mechanism may be involved in effects of ACTH and angiotensin on cyclic AMP levels.


Evidence for stimulation of renal gluconeogenesis by catecholamines. Isoproterenol and norepinephrine (10(-4) M) significantly increased cyclic AMP formation and glucose production by the isolated tubules of the renal cortex of the rat. These effects were abolished by propranolol. Theophylline diminished the effects of the catecholamines on gluconeogenesis despite a marked augmentation in cyclic AMP concentration. In the absence of calcium ion in the incubation medium, isoproterenol stimulates cyclic AMP production, but has no effect on gluconeogenesis. It is concluded that catecholamines enhance gluconeogenesis in renal cortical tubules by the stimulation of beta adrenergic receptors. This effect is probably mediated through adenyl cyclase-cyclic AMP system and requires an adequate level of ATP and the presence of calcium ion.


Tuberculosis--manifestations at uncommon sites.


Effects of a false neurotransmitter, p-hydroxynorephedrine, on the function of adrenergic neurons in hypertensive patients. Previous studies have shown that amphetamine and p-hydroxyamphetamine impair adrenergic transmission, and it has been suggested that this effect is mediated by an active metabolite, p-hydroxynorephedrine (PHN). Studies in experimental animals have shown that PHN can deplete and substitute for norepinephrine (NE) in the transmitter pool, thus meeting the criteria of a false neurotransmitter. The pharmacologic effects of PHN on adrenergic function and NE synthesis were studied in eight hypertensive patients and compared with placebo. Mean erect and supine blood pressure (BP) decreased 22/14 and 9/6 mm Hg, respectively, during PHN 600 mg daily. The post-Valsalva diastolic overshoot was abolished. The pressor sensitivity to tyramine decreased whereas the pressor response to NE was enhanced. A mild natriuresis occurred. The 24 h urinary excretion of catecholamines and catecholamine metabolites during the administration of PHN compared with placebo changed as follows: vanillylmandelic acid (VMA), 42% decrease; NE. 42% decrease; normetanephrine (NM), 400% increase: metanephrine, unchanged; dopamine, 40% decrease; while homovanillic acid was unchanged. The sum of VMA, NE, and NM decreased 23%. The posttreatment urinary excretion of PHN was biexponential with first and second phase half-lives of 13 and 55 h. respectively. The time of the second phase closely approximated the recovery of the changes in BP and excretion of VMA. No effects of PHN on the central nervous system were observed. These studies show that PHN acts peripherally to interfere with adrenergic function and NE synthesis in hypertensive patients with a resultant decrease in BP.


Intestinal absorption of phosphate in the chick: effect of vitamin D and other parameters.


Influence of dietary protein and carbohydrate levels on liver enzyme activities in quail.


Changes in the statistics of transmitter release during facilitation. 1. The statistical nature of transmitter release during facilitation was studied at single synaptic sites by recording extracellular excitatory junctional potentials from the claw opener muscle in crayfish.2. At low temperatures, single quanta could be counted in the responses to nerve impulses. The distribution of the number of quanta observed (x) was most accurately described by assuming that x is a binomial random variable.3. A quantitative estimate was made of the effects of errors in counting quanta due to the simultaneous release of quanta and the release of quanta which were not individually detectable above the noise of the recording system. Such errors of observation cannot account for the deviation of quantal release from a Poisson distribution.4. Facilitated release occurred in the responses to the second of two closely following nerve impulses and in the responses to successive impulses in a tetanus. In both cases, the increase in the average number of quanta released (m) could be attributed entirely to an increase in the probability (p) that available quanta were released.5. The results can be interpreted most easily in terms of a model in which the maximum number of releasable quanta is limited by a finite number of discrete release sites within recording distance of the microelectrode. In this model, the binomial parameter n is an estimate of the number of these sites, and the statistical parameter p is a compound probability depending on the rate of re-occupying sites after a nerve discharge and the probability that an impulse activates an occupied site.


Pre- and post-junctional effects of 1-fluoro-2,4-dinitrobenzene at the frog neuromuscular junction. 1. FDNB increased by 60-90% the depolarization of the end-plate produced by applied carbachol in frog sciatic nerve-sartorius muscle preparations.2. In partially curarized preparations, FDNB (0.4 mM) increased the amplitude of the end-plate potential by a factor of 1.8.3. The quantal content of end-plate potentials was increased by FDNB (2 mM) as determined by the method of failures.4. After approximately 25-35 min, neuromuscular transmission was blocked by 0.4 mM-FDNB, as evidenced by abolition of neurally elicited end-plate potentials. At this stage miniature end-plate potentials could still be recorded, which indicates that the neuromuscular block was presynaptic.5. FDNB (0.4 mM) increased miniature end-plate potential frequency several hundred-fold when the Ringer solution contained normal calcium concentration (1.8 mM) or 0.45 mM calcium and 5.4 mM magnesium.6. During the first 60 min of exposure to 0.4 mM-FDNB there was a slight drop (4-6 mV) in resting potentials of muscle fibres. During this period directly initiated action potentials showed a marked decrease in the rate of repolarization and a small decrease in the amplitude and rate of rise.7. Using the technique of point voltage clamping in tetrodotoxin-treated muscles, it has been found that FDNB almost completely abolished the active increase in g(K) during stepwise depolarization of the nonjunctional muscle fibre membrane from -90 to 0 mV. The passive outward leakage current appeared unaffected by FDNB.


Plasma aldosterone regulation in anephric man.


Influence of lymphography in determining extent of disease in patients with lymphoma.


Effect of Rho on transcription of bacterial operons.


Human embryonic lung cells abortively infected with Herpes virus hominis type 2 show some properties of cell transformation.


Mechanotransduction in insect neurones.

Mechanotransduction in insect neurones.


Skin as aid to neurology.

Skin as aid to neurology.


Seventeenth Bowditch lecture. Neural and humoral factors in the temporal organization of behavior.


Stimulation of splenic prostaglandin release by angiotensin and specific inhibition by cysteine 8-AII.


Steroids and related products. 38. 11-Aza steroids. 3. The synthesis of 11-aza 9 -steroids. I.


An androgen binding protein in the testis cytosol fraction of adult rats. Comparison with the androgen binding protein in the epididymis.


Steroid modifications. II. The effect of ruthenium tetroxide on several aromatic steroids.


Crystalloid intracytoplasmic inclusions in lymphocytes of a patient with chronic lymphocytic leukemia.


The diagnostic significance of enzyme estimations in clinical medicine.


Recognition of leprosy.

Recognition of leprosy.


Ultrastructure of the pars distalis of the lizard Anolis carolinensis with special reference to the identification of the gonadotropic cell.


The ultrastructure of the accessory sex organs of the male rat. I. Normal structure.


Ultrastructure of the rat neural lobe during recovery from hypertonic saline treatment.


Purification and properties of nucleases from pig pituitary gland.


Localization of oestradiol receptors in the rat hypothalamus.


Compartmental arrangement of steroids formed from (1- 14 C)acetate by rat adrenal zona glomerulosa and the effect of corticotrophin.


The biosynthesis and secretion of aldosterone by the rat adrenal zona glomerulosa, and the significance of the compartmental arrangement of steroids.


Early variations of plasma cortisol after pulse intravenous injection of dibutyryl cyclic adenosine 3',5'-monophosphate in normal subjects.


Effects of gonadotrophins and cyclic 3',5'-AMP on in vitro incorporation of ( 3 H)uridine into RNA of the prepubertal rat ovary.


Managing the menopause.

Managing the menopause.


Pathogenicity of low-virulence strains of two canine adenovirus types.


Chronic superficial keratitis in dogs: negative results of isolation procedures for Chlamydia.


Isolation of parainfluenza type 3 virus from the prepuce of bulls in Northern Queensland.


The influence of age and barbital treatment on the content of cytochrome P-450 and b 5  and on the activity of glucose-6-phosphatase in microsomes of the rat liver and kidney.


Cell-bound antiglial immunity in patients with malignant tumors of the brain.


Adenosine 3',5'-cyclic monophosphate phosphodiesterase in guinea-pig lung--properties and effect of adrenergic drugs.


Activation of a specific human plasma kininogenase with trypsin and plasmin.


Regulatory role of reducing-equivalent transfer from substrate to oxygen in the hepatic metabolism of glycerol and sorbitol.


Differences in nucleotide sequences of ribosomal RNA between the liver and a hepatoma of C3H-He mice.


The effects of thioketo substitution upon uracil-adenine interactions in polyribonucleotides. Synthesis and properties of poly (2-thiouridylic acid) and poly(2,4-dithiouridylic acid).


The induction of translocators for di- and tricarboxylic-acid anions in Azotobacter vinelandii.


The role of lysosomal enzymes in skeletal tissues.


Estimation of virus-neutralizing antibody in microplates. A simple and rapid method for the titration of virus-neutralizing antibody is described. The micro-apparatus and reagents are readily available from commercial sources. Results are included from selected cases which illustrate various conditions of immune status and response.


FL 1060: a new beta-lactam antibiotic with novel properties. The effect of FL 1060, a newly described beta-lactam antibiotic, on three strains of Escherichia coli was studied. The agent showed extraordinarily high activity as judged by conventional titrations, but inoculum size, osmolality of the medium, and length of the incubation period were all found to have a marked effect on the result. Continuous turbidimetric monitoring and microscopical observation of cultures of E. coli exposed to FL 1060 showed the effect of the antibiotic to have many novel features. Exposed bacterial rods converted to spherical forms, but in a different manner from that of classical penicillin-mediated transformation. Furthermore, although FL 1060 was bactericidal for the majority of the bacterial population, a fraction not only survived its lethal action but grew in high concentrations of the agent thereby producing a phenotypically resistant population which nonetheless continued to show characteristic morphological deformities. Possible reasons for this anomalous behaviour are discussed.


Frequency of intraendothelial 'virus-like' particles: an electron microscopy study of 376 human renal biopsies. Intraendothelial ;virus-like' particles were found in all cases requiring a renal biopsy: in 89% of patients with systemic lupus erythematosus (SLE), in 73% of those receiving renal transplants more than one year before, and in 24.5% of all other renal biopsies.


Cardiac mitochondrial activity in acute and chronic cyanosis.


Serial nitroblue tetrazolium tests in the management of infection. Two cases are described in which extensive use was made of the nitroblue tetrazolium (NBT) test. In the first case the advantages of using this technique to diagnose and control infection is shown; in the second the considerable advantage of the speed of the technique. In both of these cases the test made a material contribution to the management of the patient, and it is concluded that the test brings bacteriological control of the patient within the immediate clinical area, thus overcoming one of the principal disadvantages of the classical bacteriological methods.


Relationship of cyclic AMP to secretin stimulation of the pancreas.


Fatal measles infection in children with leukemia.


Arsenic intoxication from well water in the United States.


Effects of 3',5'-cyclic nucleotides on adrenocortical cells of hypophysectomized rats. A stereologic and autoradiographic study.


Neuropathy of long-distance running.

Neuropathy of long-distance running.


Adrenocortical response to ACTH in Addison's disease.


Intranuclear inclusion bodies.

Intranuclear inclusion bodies.


The amino-acid sequence of cytochrome c from Euglena gracilis.


The properties and amino-acid sequence of cytochrome c from Euglena gracilis.


A preliminary investigation of the swine vesicular disease epidemic in Britain.


Electron microscopic study of liver lesions, especially hepatic megalocytosis induced by 1-(4-dimethylamino-benzylidene)-indene (DABI) in mice.


The role of reticular formation in integration.


Studies on the tardigrades. 3. Fine structure of the esophagus of Milnesium tardigradum Doyère.


The digital pads of the tree frog, Hyla cinerea. I. The epidermis.


Treatment of amoebic liver abscess and amoebic dysentery with metronidazole.


Australia antigen in liver disease in Zambia.


A new method for measuring ultrasonic velocity.


Sterol and phospholipid biosynthesis in phytohemagglutinin stimulated human lymphocytes.


Pseudomonas cytochrome c peroxidase. V. Absorption spectra of the enzyme and of its compounds with ligands. Inhibition of the enzyme by cyanide and azide.


Recurring digital fibrous tumour of childhood. Case report and survey.


Fine structure of human pituitary adenoma.


Encephalomyocarditis virus (E variant) in mice. Pancreatic changes.


Nuclear DNA in salivary gland tumors. II. Adenocystic carcinomas.


Treatment of vaginal trichomoniasis with a new anti-protozoal compound ( -chloromethyl-2-methyl-5-nitro-1-imidazole-ethanol).


Delayed cutaneous metastases of mucus-producing cell carcinoma of the stomach.


Conformational studies on lipoamide dehydrogenase from pig heart. I. Interconversion of dissociable and non-dissociable forms.


Conformational studies on lipoamide dehydrogenase from pig heart. 2. Spectroscopic studies on the apoenzyme and the monomeric and dimeric forms.


Conformational studies on lipoamide dehydrogenase from pig heart. 3. The binding of sodium dodecylsulphate.


Conformational studies on lipoamide dehydrogenase from pig heart. 4. The binding of NAD +  to non-equivalent sites.


Slow conformational changes of the inorganic pyrophosphatase from bakers' yeast induced by divalent metal ions.


The initiation of protein synthesis directed by the RNA from encephalomyocarditis virus.


Suppression of testicular androgen synthesis in the rat by ethylene dimethanesulphonate.


Binding of ligands to the inorganic pyrophosphatase of bakers' yeast.


Antagonists of trophic hormones.

Antagonists of trophic hormones.


Temperature studies of the hand and its claw deformities due to peripheral nerve paralysis in leprosy.


Sarcoma of tendon sheath.

Sarcoma of tendon sheath.


Influence of time factor on the restoration of adrenal weight and cortical functions in hypophysectomized rats.


Lipolytic enzymes and the action of ACTH on lipid metabolism in rat adrenals.


Viral-host relationships in chronic diseases of man.


On the aetiology of whooping cough.

On the aetiology of whooping cough. The results of routine bacterial and viral studies on 483 patients with whooping cough, investigated at one hospital over a five year period are presented. The possible role of respiratory viruses in the aetiology of the disease is discussed.


Social class and infectious mononucleosis. The socio-economic status of 80 patients with infectious mononucleosis was compared with the socio-economic distribution of the general population in the same area of south-west London. An excess incidence of infectious mononucleosis was observed among subjects from upper socio-economic groups. A possible relationship between this observation and the epidemiology of the Epstein-Barr virus is discussed.


Epstein-Barr virus antibody in cases and contacts of infectious mononucleosis; a family study. Serological investigations were carried out on 147 patients with Paul-Bunnell positive infectious mononucleosis (IM) from the general population. All possessed antibody to the Epstein-Barr virus (EBV) and 63% showed serological evidence of recent infection. Contacts of 132 patients, 306 in all, were followed serologically; within 6 months of the index cases' illness twenty one contacts developed evidence of EBV infection or re-infection and of these five developed overt IM. The secondary attack rate of EBV infection among susceptible contacts was at least 19%; the corresponding figure for clinically apparent IM was 6%. EBV antibody prevalence among patients' siblings was significantly lower than among age-matched controls, suggesting that cases of IM come from families with a lower than normal previous experience of the virus. Of thirteen patients with persistently Paul-Bunnell negative ;glandular fever' four had serological evidence of recent EBV infection and two had probable cytomegalovirus mononucleosis. Recent EBV infection may have been associated with the illnesses of five of the remaining patients.


An analog computer study of fast, isolated movements.


Pertussis antibodies in the sera of children exposed to Bordetella pertussis by vaccination or infection. Low agglutinin titres to pertussis suspensions were found in 99% of sera from a group comprising healthy adults and non-vaccinated, non-infected infants of 1-6 months of age. These are attributable to agglutinins to heat-stable antigens and/or heat labile agglutinogen 1, and cross-absorption tests must be done on the sera in order to distinguish between the two. Agglutinins to agglutinogens 2 and 3 were found in only about 20% of adult sera. Bactericidal antibody was low in titre or absent in all sera from non-exposed individuals.Raised bactericidal antibody titres and the presence of agglutinins 2 and 3 were attributed to exposure to Bordetella pertussis antigens, either as vaccine or as infection. The variation, amongst both vaccinated and infected children, was very great. A vaccinated child who became ill responded to the infection in much the same way as a non-vaccinated child. We were unable to relate the immunity of the child to the titres either of agglutinins or of the bactericidal antibody.The protective ability of sera from vaccinated or infected children measured in mice against small, lethal brain infections was also unrelated to the state of immunity in the children, but this protective ability was correlated with the complement-mediated bactericidal antibody titres of the sera.The distribution of agglutinins, bactericidal antibody, and anti-haemagglutinin in serum IgG and IgM was different in vaccinated and infected children.


Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics.


Cell fusion induced by a virus within the zona pellucida of mouse eggs.


Effect of magnesium on horizontal cell activity in the skate retina.


Differentiation between necrotic ulcerative gingivitis and primary herpetic gingivostomatitis.


1 -Hydroxy derivative of vitamin D 3 : a highly potent analog of 1 ,25-dihydroxyvitamin D 3 . The 1alpha-hydroxy derivative of vitamin D(3) has been chemically synthesized and tested for its biological activity. This analog has comparable biological activity on a weight basis to 1,25-dihydroxyvitamin D(3) in the stimulation of intestinal calcium transport and bone calcium mobilization in normnal and anephric rats. Because the 1alpha-hydroxy derivative is synthesized from cholesterol, it is easier and less expensive to prepare than 1alpha,25-dihydroxy derivative, making it attractive as a drug in the treatment of renal osteodystrophy and hypopara-thyroidism.


Natural abundance carbon-13 nuclear magnetic resonance spectra of human serum lipoproteins. Human serum lipoproteins have been studied by Fourier transform nuclear magnetic resonance of carbon-13 in natural abundance. Spectra of highdensity, low-density, and very-low-density lipoproteins were recorded and partly assigned. The prominent features of these spectra reflect the qualitative and quantitative composition of the lipid moiety of these complexes. The results suggest that carbon-13 nuclear magnetic resonance will be a useful technique for studies of the structural and dynamic parameters of lipoproteins.


Extraction of equine infectious anemia immunodiffusion antigen with the aid of the chaotropic agent, thiocyanate. Immunodiffusion antigen from spleens of horses infected with equine infectious anemia virus was prepared by methods employing freeze-thaw cycles and thiocyanate treatment. Thiocyanate (0.5 M) permitted the recovery of the greatest amount of antigen. Furthermore, it was most effective for recovery of immunodiffusion antigen from spleens which yielded unsatisfactory concentrations of antigen by the conventional freeze-thaw or water-extraction methods. The reactivity of the antigen did not appear to be affected by this chemical treatment.


Virus susceptibility of a new simian cell line of fetal origin. The cultivation and characterization of a cell line derived from the foreskin of a fetal, rhesus monkey (rhfs2) are described. This cell line has proven satisfactory for isolation and assay of a variety of viral agents of human and simian origin. Virus titrations performed on foreskin cells yielded titers comparable to, or higher than, those obtained in rhesus monkey kidney cells (LLC-MK2). Replicate isolation attempts in our laboratory from simian clinical specimens have proven rhfs2 superior to LLC-MK2 for ease of detection and frequency of isolation.


Rapid method to determine labeling specificity of radioactive enteroviruses. The specificity of labeling enteroviruses with (32)P-labeled NaH(2)PO(4) or (14)C-leucine can be determined by comparing the percent adsorption of infective particles and radioactivity to membrane (Millipore) filters.


Evaluation of two types of infectious mononucleosis antigen slides by the indirect fluorescent-antibody technique. The efficiency of two types of antigen slides was compared by using the indirect fluorescent-antibody technique. Fifty sera from infectious mononucleosis patients were tested concurrently on the two sets of slides for antibody to Epstein-Barr virus. The indirect fluorescent-antibody serum titer readings from the epoxy slides were either equal to or twofold higher than those from the cover slip slides.


Neuraminidase induced loss in the transplantability of murine leukaemia L 1210, induction of immunoprotection and the transfer of induced immunity to normal DBA-2 mice by serum and peritoneal cells. Leukaemia L 1210 cells preincubated in vitro with neuraminidase preparations derived either from Vibrio cholerae (VCN) or Clostridium perfringens (CPN) lost their i.p. transplantability for normal DBA/2 mice. This loss of transplantability could not be observed when the enzyme treated cells were implanted in mice whose immune status was suppressed by prior cyclophosphamide treatment. Mice receiving i.p. implants of enzyme treated leukaemia cells developed immunity to subsequent i.p. challenge with untreated L 1210 cells but not to a challenge with Ehrlich ascites tumour cells. The magnitude of immune response evoked by L 1210 cells preincubated with 250 u/ml of VCN or 35 μg/ml of CPN for 60 minutes was of relatively low level when compared with the immunity induced by leukaemia cells preincubated with 50 u/ml of VCN or 15 μg/ml of CPN for 30 or 60 minutes. Evidence is presented to show that the induced immunity can be transferred passively with the serum and with the peritoneal cells from the mice implanted with VCN treated L 1210 cells to normal DBA/2 mice. The significance of the neuraminidase induced increase in the immunogenicity of treated tumour cells is discussed.


Glomus tumours.

Glomus tumours.


Human peripheral lung tumours: light and electron microscopic correlation. Thirteen human peripheral lung tumours have been studied in both light and electron microscopy. They were classified as epidermoid carcinoma, mucus-secreting cell adenocarcinoma, and alveolar cell adenocarcinoma, the latter made up of granular pneumocytes. Alveolar cell cancer, as defined by ultrastructural features, could assume different gross histological patterns in light microscopy, and therefore electron microscopy is required for its identification.Since neither squamous nor mucous metaplasia was observed in any alveolar cell tumour, it is tentatively suggested that all peripheral lung tumours which lack these features may be derived from granular pneumocytes, irrespective of whether they appear to be adenocarcinomata or large cell carcinomata when examined by light microscopy.


Glioblastoma multiforme: a controlled trial to assess the value of specific active immunotherapy in patients treated by radical surgery and radiotherapy. The results are reported of a randomized prospective clinical trial carried out to assess the value of specific active immunotherapy using irradiated autologous tumour cells in patients with glioblastoma multiforme treated by radical surgery and post-operative irradiation. The results in 62 patients show no statistically significant difference in survival between the group receiving adjuvant autologous tumour cells and those treated with surgery and radiotherapy alone. All 27 patients receiving tumour cells were dead at 30 months, whereas 7 of the 35 controls were alive at this time. The results were considered sufficiently discouraging to abandon the trial at this stage on the grounds that there was sufficient evidence in this study that the administration of irradiated autologous cells was of no benefit to patients with high grade astrocytomata.


Early diagnosis of nephroblastoma.

Early diagnosis of nephroblastoma.


Persistent haemagglutination for infectious mononucleosis in rheumatoid arthritis.


Infection with E.B. virus.

Infection with E.B. virus.


Comparative trial of P1496, a new non-steroidal oestrogen analogue. The results are reported of a preliminary trial of P1496, a new non-steroidal oestrogen analogue, compared with a conjugated equine oestrogen and a placebo. The oestrogenicity of both substances was well substantiated by vaginal epithelial maturation indices. P1496 was superior to conjugated equine oestrogen in producing a significant reduction of plasma calcium levels and a possible reduction in serum cholesterol. Conjugated oestrogen caused slightly more nausea than P1496 but there were no notable side effects from either drug. P1496 is considered to be at least as effective an oestrogenic substance as conjugated oestrogen and worthy of further therapeutic evaluation.


Recovery of adrenal function after substitution of beclomethasone dipropionate for oral corticosteroids. Of 16 steroid-dependent asthmatic patients oral treatment has been discontinued in six and reduced in four after the introduction of beclomethasone dipropionate. Substitution of inhaled beclomethasone for oral steroids was unsuccessful in the remaining six patients. Serial adrenal function studies in the patients whose oral treatment was discontinued showed progressive recovery, and five out of six had a normal response to tetracosactrin stimulation after two months.


Herpes simplex and zoster.

Herpes simplex and zoster.


Determination of IgG heavy chains by electroimmunodiffusion in the nanogram range: simultaneous application to serum and CSF of neurological patients.


Plasma lipids in patients with chronic renal failure.


The diagnostic value of thrombocytic glucose-6-phosphatase in patients with von Gierke's disease and its heterozygotes.


Histochemical and histological studies of rat myocardium following mechanical compression.


Crystalloid inclusions in mitochondria of renal tubules.


Electron spin resonance of spin labels in organic inclusion crystals. Models for anisotropic motion in biological membranes.


Growth and its regulation in the healthy child.


A theoretical model for the effects of local nonpolar heme environments on the redox potentials in cytochromes.


A convenient synthesis of 1 -hydroxy-vitamin D 3 .


Hindlimb muscle fiber populations of five mammals.


Different patterns of neurologic involvement with herpes simplex virus types 1 and 2: isolation of herpes simplex virus type 2 from the buffy coat of two adults with meningitis.


The limited effect of nasal interferon induced by rhinovirus and a topical chemical inducer on the course of infection.


Inhibition of oral shedding of herpes simplex virus by cytosine arabinoside.


Impaired production of interferon in lymphocytes from immunosuppressed patients.


Adenoid cystic carcinoma of the ear canal.


Leukocyte-transforming agent: prolonged excretion by patients with mononucleosis and excretion by normal individuals.


Survey for antibodies to canine viruses in selected wild mammals.


Lipid metabolism in pregnancy. I. Changes in lipoprotein triglyceride and cholesterol in normal pregnancy and the effects of diabetes mellitus.


Ultrasonic visualization in determination of tumor growth rate.


Prism adaptation and brain damage.

Prism adaptation and brain damage.


Indium-113 m generator using alumina or silica gel.


RNA-directed DNA synthesis and RNA tumor viruses.


Oxygenated cytochrome P-450 as an intermediate in hydroxylation reactions.


Characteristics of tumors induced in mammals, especially rodents, by viruses of the avian leukosis sarcoma group.


Dipyrone in bronchial asthma.

Dipyrone in bronchial asthma.


Association between malignant disease in children and maternal virus infections. The possibility of an association between virus infections during pregnancy and subsequent malignant disease in the child has been investigated using retrospective data from the Oxford Survey of Childhood Cancers. Such an association appears to exist for influenza, chickenpox, and possibly rubella. For influenza and rubella the estimated risk is small; the data do not permit an estimate to be made directly in the case of chickenpox. It is suggested that there may be a specific association between maternal chickenpox and tumours of the nervous system.It is important to emphasize that, even if the relative risk associated with these viruses is fairly large, the number of cases of childhood cancer and leukaemia actually attributable to them is probably very small.


Proteolytic activity of Entamoeba histolytica. 3. Some properties of the enzyme.


Virus-erythrocyte interactions.

Virus-erythrocyte interactions.


Antipsychotic drug actions: a clue to the neuropathology of schizophrenia?


A proton magnetic resonance study of the conformations of 3',5'-cyclic nucleotides.


A  13 C and  1 H nuclear magnetic resonance study of the conformations of 2',3'-cyclic nucleotides.


Enzymatic preparation of an optically active phosphotriester, asymmetric only at phosphorus.


The effect of cycloheximide on the interaction between mitochondrial respiration and gluconeogenesis in guinea pig and rat liver.


The regulation of nicotinamide adenine dinucleotide-linked substrate oxidation in isolated liver mitochondria.


A class I (Schiff base) fructose diphosphate aldolase of prokaryotic origin. Purification and properties of Micrococcus aerogenes aldolase.


Structural comparisons between the class I fructose diphosphate aldolases from Micrococcus aerogenes and rabbit.


Enzymatic epoxidation. II. Comparison between the epoxidation and hydroxylation reactions catalyzed by the  -hydroxylation system of Pseudomonas oleovorans.


Purification and properties of the colicin E3 receptor of Escherichia coli.


Formation of monoenoic fatty acids by desaturation in rat brain homogenate. Some properties of the enzyme system of 10-day-old brain.


Regulation of adenosine 3',5'-monophosphate-dependent protein kinase. I. Preliminary characterization of the adipose tissue enzyme in crude extracts.


Regulation of adenosine 3',5'-monophosphate-dependent protein kinase. II. Hormonal regulation of the adipose tissue enzyme.


Squalene synthetase. II. Purification and properties of bakers' yeast enzyme.


Squalene synthetase. 3. Mechanism of the reaction.


Conditionally lethal mutations in Chinese hamster cells. I. Isolation of a temperature-sensitive line and its investigation by cell cycle studies.


Differential blood lipid pattern in diabetics and atherosclerotics.


Viruses causing common respiratory infections in man.


The ability of calcium to change cyclic AMP from a stimulator to an inhibitor to thymic lymphoblast proliferation.


Investigations of the mechanism of decreased tumorigenicity of cells grown in BrdU.


Endocrine causes of hypertension.

Endocrine causes of hypertension.


Ultrastructural cytopathology of mouse myocardium associated with EMC viral infection.


Adenylate kinase in human tissue. II. Serum adenylate kinase and myocardial infarction.


Patient participation in ward program planning.


Identification of laboratory animals: freeze marking.


The institutional milieu as a work environment.


Automated nursing notes in a psychiatric institution.


Aspects of the Petrie test in psychiatric patients.


Immunologic renal disease in laboratory animals.


Constraints and opportunities in a nursing career.


Conjoint therapy as an adjunct to individual therapy.


The plasmid of Salmonella typhimurium LT2.


Phobia: a private experience.

Phobia: a private experience.


A patient with multiple insulinomata.

A patient with multiple insulinomata.


Catecholamine and Ca 2+  activation of adenylate cyclase systems in synaptosomal fractions from rat cerebral cortex.


Thymidine kinase activity in Burkitt lymphoblastoid somatic cell hybrids after induction of the EB virus.


An old fashioned method to deal with delinquent behavior: more effective than courts?


Syncytial giant cell focus assay for viruses derived from feline leukemia and a simian sarcoma.


Effect of zinc on the viability and phagocytic capacity of peritoneal macrophages.


Medicare--nursing home benefits.

Medicare--nursing home benefits.


Study on plasma cyclic nucleotide concentrations in fasting rats.


The effects of an acid pH upon the induction of antiviral resistance in vitro by interferon.


Virus resistance in rabbit kidney cell cultures contaminated by a protozoan resembling Encephalitozoon cuniculi.


Nurses' attitude toward heroin addicts.

Nurses' attitude toward heroin addicts.


Regulation of myocardial prostaglandin dehydrogenase activity. The role of cyclic 3',5'-AMP and calcium ions.


Charge heterogeneity of human low density lipoprotein (LDL).


Effect of angiotensin II on plasma free fatty acids in hypertensive subjects with and without overweight.


Luxation and ligamentous injuries of the elbow of the dog.


Vitamin D. Metabolism and mechanism of action.


Immunity and immunopathology in virus infections.


On the prevention of virus induced blockade of cell protein synthesis by specific inhibitors of poliovirus growth.


Double drug-dependence in a poliovirus strain.


Electromagnetic measurement of iliac artery blood flow in the rat: on-line integration of flow.


Multiple sclerosis: an immunologic reassessment.


Chemical stimulation of the brain: relationship between neural activity and water ingestion in the rat.


Mapping and characerization of mutants of Pseudomonas aeruginosa affected in nitrate respiration in aerobic or anaerobic growth.


Localized muscle fatigue--definiton and measurement.


Metastatic carcinomas involving the testis: a review.


Serological survey of rhinovirus infections in Japan.


A case of massive hypertriglyceridemia corrected by nicotinic acid or nicotinamide therapy.


Effect of chlorophenoxyisobutyric acid (CPIB) on fat-mobilizing lipolysis and cyclic AMP levels in rat epididymal fat.


Antagonism of non-inducer ribonucleic acid against induction of antiviral cell resistance and interferon by inducer ribonucleic acid.


Isolation and immunological characterization of human glomerular basement membrane antigens.


The effect of a reversed cycle of sleep and wakefulness on diurnal EEG patterns in rhesus monkeys.


Carcino-foetal human liver ferritins.

Carcino-foetal human liver ferritins.


Damage to microsomal membrane by lipid peroxidation.


The use of phospholipid film for shaping cell cultures.


Comparison of ribosomal protein synthesis in Becker and Duchenne muscular dystrophies.


Cerebral dysautoregulation in central neurogenic orthostatic hypotension (Shy-Drager syndrome).


On thalamic substrates of cortical synchrony.


Electron spin resonance studies of  -irradiated dinucleotides and related compounds at 77 degrees K.


The REM sleep-inducing action of a naturally occurring organic bromine compound in the encéphale isolé cat.


Hepatic tumours in infancy and childhood.


Neurophysiological studies on muscle function in the stump of above-knee amputees.


Multiple virus isolations from a pregnant patient.


Polygraphic aspects of sleep in man.

Polygraphic aspects of sleep in man.


Principles of automatic analysis of sleep records with a hybrid system.


Hereditary neuronal abiotrophy in the Swedish Lapland dog.


Multiple effects of glucocorticoids on TSH secretion in unanesthetized rats.


Apnoeic periods and the respiratory related arousal patterns during sleep in the Pickwickian syndrome. A polygraphic study.


Stimulation of the adenyl cyclase-cyclic AMP system in the thyroid of the rat.


Corticosteroid feedback control of ACTH secretion: effect of stress-induced corticosterone ssecretion on subsequent stress responses in the rat.


Insomnia, narcolepsy, and sleep apneas.

Insomnia, narcolepsy, and sleep apneas.


Effects of estrogen treatment on plasma renin parameters in the rat.


The binding of 18-hydroxydeoxycorticosterone and 18-hydroxycorticosterone to mineralocorticoid and glucocorticoid receptors in the rat kidney.


Hypersomnia with periodic breathing: periodic apneas and alveolar hypoventilation during sleep.


Pinealectomy and brain myelination.

Pinealectomy and brain myelination.


Evidence for a new class of corticosterone receptors in the rat kidney.


Tracheostomy in hypersomnia with periodic breathing.


Continuous recording of the pulmonary and systemic arterial pressure during sleep in syndromes of hypersomnia with periodic breathing.


Role of pancreatic monoamines in the impaired insulin secretion of the fasting state.


Effect of adrenocorticotropic hormone on extracellular adenosine 3',5'-monophosphate in the hypophysectomized rat.


The metabolism of perchlorate in the rat.


Nuclear receptor-estrogen complex: relationship between concentration and early uterotrophic responses.


Effect of hypophysectomy on serum dopamine-beta-hydroxylase activity in rat.


Hydrochlorothiazide therapy in nephrolithiasis. Effect on the urinary activity product and formation product of brushite.


Peripheral levels of estrogen in the PMS-primed cycling hamster.


Ouabain stimulation of  45 CA ++  accumulation in the rat pituitary.


Hormonal factors influencing ovarian uptake of human chorionic gonadotropin.


Anomalous prolactin release in vitro in response to cold and its specific blockade by a purified hypothalamic-inhibiting factor.


Characterization of human corneal collagenase.


Discharge plans--the weakest link.

Discharge plans--the weakest link.


Study of cellular inclusion in the human foetal intestinal neuron.


Prevalence of amoebiasis and other intestinal parasitic infections in a selected community.


Evaluation of laboratory methods in the detection of E. histolytica and other intestinal parasites.


Does entrapment neuropathy contribute to nerve damage in leprosy?


The use of cord serum and indirect viral immunofluorescence as a method of evaluating the immunological specificity of fluorescein-labelled anti-human IgA conjugate.


Coagulation and renal disease.

Coagulation and renal disease.


A comparison of the antilipolytic effects of nicotinic acid, methylisoxazole carboxylic acid, methylpyrazole carboxylic acid and insulin in isolated fat cells.


A novel assay for erythrocyte carbonic anhydrase and certain sulfonamide drugs employing spin-labels.


The effect of corticotrophin on the compartmental arrangement of steroids, and the control of steroid hormone secretion by the rat adrenal cortex in vitro.


Preparation of oestriol-6-(O-carboxymethyl) oxime and oestrone-6-(carboxymethyl) oxime-bovine serum albumin conjugates.


On the oscillatory potentials of the human electroretinogram in light and dark adaptation.


A reappraisal of histopathology in lung cancer and correlation of cell types with antecedent cigarette smoking.


Subcellular location of the kininogenase in the coagulating gland of the guinea-pig.


Studies on cytochrome c. V. Synthesis of the protected decapeptide (sequence 57-66) of Baker's yeast iso-1-cytochrome c.


Studies on cytochrome c. VII. Synthesis of the protected undecapeptide (sequence 82-92) of Baker's yeast iso-1-cytochrome c.


Studies on cytochrome c. VI. Synthesis of the protected pentadecapeptide (sequence 64-81) of Baker's yeast iso-1-cytochrome c.


Actinomycin D for Wilm's tumor.

Actinomycin D for Wilm's tumor.


Studies on cytochrome c. 8. Synthesis of the protected hexadecapeptide (sequence 93-108) of Baker's yeast iso-1-cytochrome c.


Glomus tumours.

Glomus tumours.


Vincristine neuropathy. Clinical and electrophysiological observations.


Phagocytosis by alveolar macrophages: pharmacologic features.


Retarded clearance of macroaggregated albumin from the lung of asthmatic patients.


Role of viral infection and host factors in acute episodes of asthma and chronic bronchitis.


Quantitative analysis of phenolic compounds after thin-layer chromatographic separation.


Thin-layer chromatography for quantitative polychlorinated biphenyl analysis.


The juvenile angiofibroma of the nasopharynx.


Effect of hypophysectomy, isletectomy and ACTH on glycemia and hematocrit in the bullhead, Ictalurus melas.


Studies on carcinogenic bifunctional aminoazo dyes. IV. Progressive microscopic alternation in the livers of rats fed the carcinogenic bifunctional aminoazo dyes: (N', N'-dimethyl-4'-amino)-N-acetyl-N-monomethyl-4-aminoazobenzene.


Cyclic AMP and its binding protein in the stimulated thyroid of the rat.


Thermoregulatory effects of prostaglandins E 1 , E 2 , F 1   and F 2   in the sheep.


3',5'-cyclic AMP phosphodiesterases in cytosol of uterine smooth muscle; effects of angiotensin.


Tumor immunology.

Tumor immunology.


Histocompatibility antigens and species-specific antigens.


Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production.


Induction of specific unresponsiveness with the aid of Bordetella pertussis.


Isolation of islets of Langerhans for transplantation.


Role of virus infections in immunosuppressed renal transplant patients.


Cytotoxicity of Rous sarcoma cells by lymphoid cells in Japanese quails.


Depressed allograft immunity in leukemia virus infected mice.


The Epstein-Barr Virus (EBV) and Marek's Disease.


Optimal growth conditions for (Ibadan) strains of Entamoeba histolytica.


Role of hormones of adrenal cortex and ovaries of pregnant mother in stress reaction of rat fetus.


The extra-articular features of rheumatoid arthritis. A systematic analysis of 127 cases.


A mucopolysaccharide storage disease with involvement of the renal glomerular epithelium.


Corticosteroidogenesis in isolated adrenal cell preparations of vitamin E-deficient rats.


The dynamics of vitamin E transport in the human erythrocyte.


Hepatocarcinoma with biliary obstruction. Report of a case and review of the literature.


The cellular mechanisms of vitamin E action: direct and indirect effects of  -tocopherol on mitochondrial respiration.


Pathogenesis of demyelination induced by a mouse hepatitis.


Nuclear DNA in salivary gland tumors. I. Warthin tumors, benign mixed tumors, and mucoepidermoid carcinomas.


Fine structure of Toxoplasma in the human brain.


Peliosis hepatis. Possible etiologic role of anabolic steroids.


Urea-induced inactivation and dissociation of NAD-specific glutamate dehydrogenase of Neurospora and its reversal by substrates.


The electron paramagnetic resonance spectrum of ferricytochrome c and lysine-modified derivatives at alkaline pH.


The pH-induced transition of iodinated ferricytochrome c: electron paramagnetic resonance and absorption spectra.


A comparison of the secretion of two components of very low density lipoproteins by perfused rat liver.


The technique and applications of lymphography. The technique of radiographic examination by lymphography of normal lymphatic structures regional to the hind limbs is described in the rabbit, dog and rhesus monkey. Radiographic appearances of normal lymph nodes and possible applications to veterinary science are indicated.Normal lymph node microstructure is illustrated using in vivo intralymphatic injection of a brightly colored radiopaque silicone rubber. Angiogenesis in active germinal follicles during the period of an immune response is displayed. Further applications of the casting technique are suggested.


Collagenolytic activity of gingivae from alloxan-diabetic rats.


Effect of morphine on the transport of dopamine into mouse brain slices.


Effects of angiotensin-II on myocardial mechanics and contractile state of heart muscle.


Nicotinic acid and nicotinamide adenine dinucleotide (NAD) therapy in schizophrenia: a review.


The biphasic response of the isolated guinea-pig ileum by bradykinin.


Effect of angiotensin on the response to norepinephrine and peri-arterial stimulation of the isolated perfused cat terminal ileum.


N-methyl bicuculline and primary afferent depolarization.


The cytomegaloviruses and the eye.

The cytomegaloviruses and the eye.


Characterization of bovine serum lipoproteins.


Isolation and partial characterization of temperature-sensitive mutants of the SAT-1 strain of foot-and-mouth disease virus.


Preparation and metabolism of isolated cells from bovine adipose tissue.


Double antibody radioimmunoassay of renin activity and angiotensin II in human peripheral plasma.


Electron microscopic study of ATPase activity in human brain tumors.


Myoneural and intermuscular junctions in a molluscan smooth muscle.


Stereotaxic diagnosis and treatment of intracranial tumors including an initial experience with cryosurgery for pinealomas.


The effect of Triton X-100 on the ultrastructure of ejaculated bovine sperm.


Lithium-induced ADH resistance in toad urinary bladders.


The choice of therapy in the treatment of malignancy.


Amyloid in endocrine tumors.

Amyloid in endocrine tumors.


The use of drugs in combination for the treatment of cancer: rationale and results.


Marginal vitamin B 12  intake during gestation in the rat has long term effects on the offspring.


A simple method for estimating H 1  in ESR experiments--the microwave power saturation of  -irradiation induced glycylglycine radicals.


Effect of dieldrin on hepatic carbohydrate metabolism and protein biosynthesis in vivo.


Effect of colloidal silicic acid on lecithin bilayers.


Bilayer lipid membrane as a model for vasopressin, prostaglandin and Ca 2+  effects on water permeability.


Plasma membranes from fibroblastic cells in culture. Isolation, morphological and enzymatic identification.


Enzyme activities in membranes from three phenotypes of the murine plasmocytoma MOPC 173, cultivated in vitro.


Metabolically controlled hemolysis of chicken erythrocytes.


Isolation of pig platelet membranes and granules. Distribution and validity of marker enzymes.


Studies of neoplastic myelomonocytic cells in BALB-c mice producing infectious C-type viruses.


Ultrastructural studies of histiocyte-tumor cell interactions during tumor regression after intralesional injection of Mycobacterium bovis.


Characterization of glycogen synthetases and phosphorylases in transplantable rat hepatomas.


Enhancement of virus-induced leukemias and sarcomas in F 1  hybrid mice inoculated with parental spleen cells.


Independence of pituitary tumor-mediated suppression of growth hormone secretion and cyclic 3',5'-adenosine monophosphate production.


Sulfated mucopolysaccharide production by synovial sarcoma cells in vivo and in tissue culture.


Suppression of pituitary tumor growth and function by ergot alkaloids.


Chromatographic studies of tyrosyl and phenylalanyl transfer RNA's of liver and tumor cells.


Effects of age on patterns of enzyme adaptation in male and female rats.


Octahedral crystals in Phycomyces. II.

Octahedral crystals in Phycomyces. II. "Phycomyces blakesleeanus" sporangiophores contain octahedral crystals throughout their cytoplasm and vacuole. More octahedral crystals were found in the wild-type strain G5 (+) than in the beta-carotene-deficient mutant C5 (-), and much more than in the mutant C141 (-), which is sensitive to only high light intensity. In the wild type, the number of crystals per sporangiophore increased until the sporangiophore reached stage IV, and then decreased. Stage I contained the most crystals per unit volume. Cultures grown in darkness had the maximum number of crystals. Under high light intensity, there was an overall reduction of crystals. The crystals are regular octahedrons. The crystals were isolated from the sporangiophores by a method of sucrose density-gradient centrifugation. They contain nearly 95% protein, are stable in organic solvents, but can be solubilized in buffer solution above pH 9.5 and below 2.5. The crystals weakly fluoresce with an emission peak at 540 nm, which is affected by irradiation with white light. Absorption spectra of freshly prepared crystals show absorption maxima around 265-285 nm, 350-380 nm, and 450-470 nm. These absorption peaks for the crystals are close to those of the phototropic and light-growth action spectra. These data suggest that the crystals may contain a flavoprotein which may be the photoreceptor pigment of "Phycomyces".


Appearance and distribution of ferritin in mouse peritoneal macrophages in vitro after uptake of heterologous erythrocytes. Mouse peritoneal macrophages have been studied in vitro after ingestion of treated rat, rabbit, or sheep erythrocytes. Under light microscopy, phagocytic vacuoles persist up to 24 h. Macrophages lose benzidine reactivity about 5 h after red cell ingestion, and they become prussian blue positive at 2 days. Ultrastructural studies show little or no ferritin in control macrophages not fed erythrocytes. In contrast, after red cell ingestion, ferritin is widely distributed in the cytoplasmic matrix and in some cytoplasmic granules by 48 h. The Golgi complex, pinocytic vacuoles, endoplasmic reticulum, nuclei, and mitochondria do not contain ferritin. Between 2 and 4 days, ferritin in cytoplasmic granules increases, concomitant with decrease in the ferritin in the cytoplasmic matrix. Evidence is presented suggesting that ferritin in the cytoplasmic matrix is translocated into cytoplasmic granules by autophagy. Polyacrylamide gel studies on macrophages after uptake of red blood cells labeled with radioiron confirm that macrophages produce radiolabeled ferritin by 4 days.


Enhancement of Sendai virus-mediated cell fusion by cupric ions. The effect of divalent cations on cell fusion by concentrated Sendai virus, inactivated by beta-propiolactone, was investigated using Vero and mouse L-929 cells in monolayers. With both cell lines, which are normally resistant to exogenous viral fusion, Cu(2+) in sublethal concentrations was found to promote polykaryon formation to a marked degree. The simultaneous presence of Cu(2+) and virus was required for this effect, which was thought to be related to the cytotoxic action of Cu(2+) on the cell membrane. Accordingly, under standard conditions and in the absence of virus, leakage of isotopically labeled intracellular protein was shown to bear a quantitative relationship to Cu(2+) concentration. Concomitant changes in the membrane were seen electron microscopically to consist of loss of microvilli and the appearance of numerous vesicles on, or adjacent to, the membrane. The relationship of enhanced fusibility to these toxic changes was not further elucidated. The fusion-promoting effect of Cu(2+) far exceeded that of Ca(2+); and other cations tested had no effect.


Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction. When the nerves of isolated frog sartorius muscles were stimulated at 10 Hz, synaptic vesicles in the motor nerve terminals became transiently depleted. This depletion apparently resulted from a redistribution rather than disappearance of synaptic vesicle membrane, since the total amount of membrane comprising these nerve terminals remained constant during stimulation. At 1 min of stimulation, the 30% depletion in synaptic vesicle membrane was nearly balanced by an increase in plasma membrane, suggesting that vesicle membrane rapidly moved to the surface as it might if vesicles released their content of transmitter by exocytosis. After 15 min of stimulation, the 60% depletion of synaptic vesicle membrane was largely balanced by the appearance of numerous irregular membrane-walled cisternae inside the terminals, suggesting that vesicle membrane was retrieved from the surface as cisternae. When muscles were rested after 15 min of stimulation, cisternae disappeared and synaptic vesicles reappeared, suggesting that cisternae divided to form new synaptic vesicles so that the original vesicle membrane was now recycled into new synaptic vesicles. When muscles were soaked in horseradish peroxidase (HRP), this tracerfirst entered the cisternae which formed during stimulation and then entered a large proportion of the synaptic vesicles which reappeared during rest, strengthening the idea that synaptic vesicle membrane added to the surface was retrieved as cisternae which subsequently divided to form new vesicles. When muscles containing HRP in synaptic vesicles were washed to remove extracellular HRP and restimulated, HRP disappeared from vesicles without appearing in the new cisternae formed during the second stimulation, confirming that a one-way recycling of synaptic membrane, from the surface through cisternae to new vesicles, was occurring. Coated vesicles apparently represented the actual mechanism for retrieval of synaptic vesicle membrane from the plasma membrane, because during nerve stimulation they proliferated at regions of the nerve terminals covered by Schwann processes, took up peroxidase, and appeared in various stages of coalescence with cisternae. In contrast, synaptic vesicles did not appear to return directly from the surface to form cisternae, and cisternae themselves never appeared directly connected to the surface. Thus, during stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vesicles by way of intermediate cisternae.


Growth inhibition of murine tumor cells, in vitro, by puromycin, ( 6 N)O 2 '-dibutyryl 3',5'-adenosine monophosphate, or adenosine: evidence of commitment for cell division. The cytostatic effects of puromycm, [(6)N]O(2')-dibutyryl 3',5'-adenosine monophosphate, and adenosine on asynchronous and synchronous cultures of the murine mastocytoma, P815Y, have been studied. Cell growth was arrested after a minimum of one further division. A model is proposed for the inhibition of cell division in which the periods of inhibition and growth arrest are separated in time by one cell cycle.


Subcellular morphometric and biochemical analysis of developing rat hepatocytes. Livers of rats between the 16th gestational and 100th postnatal day of age were subjected to quantitative biochemical and electron microscope, morphometric analyses. The amount of total mitochondrial protein per gram of liver remained at 34% of the adult level throughout the last 4 days of gestation but this was the period of rapid rise in the levels of cytochrome c oxidase, aspartate aminotransferase, and glutamate dehydrogenase in mitochondria; the nuclear fraction also acquired some glutamate dehydrogenase but lost most of it during postnatal development. During early postnatal life the amount of mitochondrial protein rose in parallel with the levels of cytochrome c oxidase and glutamate dehydrogenase but the upsurges of glutaminase and, later, of ornithine aminotransferase were accompanied by relatively little change in total mitochondrial protein. The surface area of rough endoplasmic reticulum per unit volume of hepatocyte cytoplasm (S(v) (RER)) did not change significantly throughout the period of development studied. From the 16th day of gestation to term the surface area of smooth ER (S(v) (SER)), the volume occupied by mitochondria (V(v) (MT)) and their number (N(v) (MT)) remained at 30, 66, and 45% of their adult values, respectively. V(v) (MT) and N(v) (MT) attained their maximal levels by the 2nd postnatal day and S(v) (SER) between days 2 and 12. Mitochondria of adult liver are thus smaller and contain more protein per unit volume than do those of fetal liver. After the 12th postnatal day, hepatocytes treble their size; they acquire more cytoplasm with additional enzymes but without further change in organelle concentration. The data reveal several distinct phases in the differentiation of hepatocytes. Each phase can be characterized by the extent to which the quantity and composition of various subcellular compartments evolve.


Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction. Curarized cutaneous pectoris nerve-muscle preparations from frogs were stimulated at 10/s or at 2/s for periods ranging from 20 min to 4 h. End plate potential were recorded intracellularly and used to estimate the quantity of transmitter secreted during the period of stimulation. At the ends of the periods of stimulation the preparations were either fixed for electron microscopy or treated with black widow spider venom to determine the quantities of transmitter remainind in the terminal. Horseradish peroxidase or dextran was added to the bathing solution and used as a tracer to detect the formation of vesicles from the axolemma. During 4 h of stimulation at 2/s many new vesicles were formed from the axolemma and the quantity of transmitter secreted was several times greater than the quantity in the initial store. After this period of stimulation, the terminals were severely depleted of transmitter, but not of vesicles, and their general morphological organization was normal. During 20 min of stimulation at 10/s the nerve terminals swelled and were severely depleted both of vesicles and of transmitter. During a subsequent hour of rest the changes in morphology were largely reversed, many new vesicles were formed from the axolemma and the stores of transmitter were partially replenished. These results suggest (a) that synaptic vesicles fuse with, and re-form from, the membrane of the nerve terminal during and after stimulation and (b), that the re-formed vesicles can store and release transmitter.


Cyclic adenosine monophosphate in phagocytizing granulocytes and alveolar macrophages.


The effect of poly-L-lysine on the uptake of reovirus double-stranded RNA in macrophages in vitro. The effect of polycations on cultured mouse peitoneal macrophages has been examined. Polycations, at concentrations greater than 5 microg/ml, are toxic for macrophages) as measured by failure of the cells to exclude vital dyes. At toxic concentrations polycations bind in large amounts to nuclei and endoplasmic reticulum, while at nontoxic levels polycations bind selectively to the cell surface. Nontoxic concentrations of polycations stimulate binding of reovirus double-stranded (ds) RNA to the macrophages by forming polycation-dsRNA complexes either in the medium or at the cell surface. These complexes enter the cell in endocytic vacuoles and are concentrated in secondary lysosomes. Despite exposure to the acid hydrolases within this cell compartment, the dsRNA and the polycation (poly-L-lysine) are conserved in a macromolecular form within the vacuolar system. The mechanism(s) by which the uptake of infectious nucleic acids and the induction of interferon by dsRNA are stimulated by polycations are discussed.


Transmission of maternal antibodies to Sendai virus in mice and its significance in enzootic infection.


Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map.


The possible nature of the chance-event in initiation of virus infection at the cellular level.


Genetic interactions between temperature-sensitive mutants of types 1 and 2 herpes simplex viruses.


A practical and quantitative microtest for determination of neutralizing antibodies against Epstein-Barr virus.


Oncogenicity of non-transforming mutants of avian sarcoma viruses.


An infectious centres technique for the assay of infectious polyoma virus DNA.


Comparative studies of the mitochondrial properties of Longissimus dorsi muscles of Pietrain and Large White pigs.


The effect of diarrhoea on low density beta lipoproteins and cholesterol in protein calorie malnutrition.


Low-renin hypertension.

Low-renin hypertension.


Metaplastic breast carcinoma. Rare form of mammary cancer.


Hemochromatosis, hepatoma, erythrocytosis, and erythropoietin.


Gentamicin therapy for liver abscesses due to P. aeruginosa and S. marcescens.


Ultraviolet photobleaching of free radicals created in  -irradiated amino acids.


Free radicals in irradiated nitro-substituted pyrimidines.


Electron spin resonance of x-irradiated single crystals of L-threonine at 77 degrees K.


Effect of FSH on the in vivo uptake of  3 H-testosterone by rat seminiferous tubules.


Investigation of the metabolism of 3-methoxy-17-epioestriol.


Studies of the potency of polypeptides with ACTH action by a new method based on continuous measurement of plasma corticosterone.


Mitochondrial crystals in muscles of a patient with spastic entropion.


Ceroid-lipofuscinosis (Batten's disease). First ophthalmological report of cytoplasmic inclusions in the Schwann's cell of the sural nerve in two patients with an amaurotic familial idiocy.


Return of goblet cells after vitamin A therapy in xerosis of the conjunctiva.


Hydrolytic enzyme substrates. I. Chemical synthesis and characterization.


Hydrolytic enzyme substrates. 3. Phosphomonoesterase substrates.


Hydrolytic enzyme substrates. II. A new method for measuring periodate.


Room-temperature chromatography of nucleic acids on hydroxylapatite columns in the presence of formamide.


Predictability of response to endocrine ablation in advanced breast carcinoma. A correlation to estrogen receptor and steroid sulfurylation.


Model studies on the effects of neutral salts on the conformational stability of biological macromolecules. II. Effects of vicinal hydrophobic groups on the specificity of binding of ions to amide groups.


Model studies on the effects of neutral salts on the conformational stability of biological macromolecules. 3. Solubility of fatty acid amides in ionic solutions.


Control of glucose-metabolizing enzymes in preneoplastic rat liver.


Model studies on the effects of neutral salts on the conformational stability of biological macromolecules. IV. Properties of fatty acid amide micelles.


Interaction of an apolipoprotein (apoLP-alanine) with phosphatidylcholine.


Oxidative modification of proteins in the presence of ferrous ion and air. Effect of ionic constituents of the reaction medium on the nature of the oxidation products.


Respiration of rat lung mitochondria and the influence of Ca 2+  on substrate utilization.


Ionic partition between surface and bulk water in a silica gel. A biological model. Distribution of the biologically important ions between two aqueous phases of different structure has been used as a model for ionic distribution in living tissue. When other sources of specificity had been eliminated or corrected for, surface-oriented water in a silica gel was found to have increased solvent power for water-structure-breaking ions and decreased solvent power for water-structure-making ions; and the relative solubility of an ion in the phase of enhanced structure increased regularly with the water-structure-breaking powers of the ion. The ionic selectivity was decreased in the presence of urea. The selectivity of the gel water for potassium relative to sodium increased to a maximum when the gel surface was partially ionized so that distribution of cations was not linked to distribution of anions, and then decreased as the surface changed from a hydrogen bonding to an ionic surface. It is pointed out that the distribution of ions across most living cell membranes is qualitatively the same as that found in this silica gel, and it is suggested that the membrane separates two aqueous phases of different structure, and that the enhanced structure of cell water contributes to the observed ionic distributions.


Identification of a viral inhibitor in ground beef.


Cyanogen bromide peptides from insoluble skin and dentin bovine collagens.


Inhibition of polyoma virus development in mouse-hamster heterokaryons.


Rapid extraction and physical detection of polyoma virus.


The secondary effects of sterilization of the rifampicin by gamma irradiation.


Interactions between calcium and barium on the spontaneous release of transmitter from mammalian motor nerve terminals.


Correlation between amplitudes and rise times of the miniature endplate potentials in frog muscle.


'Hallucinogenic drugs and central transmitter substances'.


A neural network with a background level of excitation in the cat hippocampus.


Interference of uranyl chloride with structure and function of molecules active at the central nervous system level.


Metabolite levels and enzyme activities in the bovine mammary gland at different stages of lactation. I. Metabolite levels related to energy production.


Cytomegalovirus latency in cultured human cells.


An investigation of factors influencing the susceptibility of Balb-c 3T3 cells to polyoma virus.


Cyclic variation in susceptibility of Balb-c 3T3 cells to polyoma virus.


The kinetics of DEAE-dextran-induced cell sensitization to transfection.


The production of infective virus by SV40-transformed human renal cells.


Virus antigens on the surface of infected cells: binding and elution of ( 125 I)-labelled antivirus antibody.


Distortion of poliovirus particles by fixation with formaldehyde.


Angiotensin antagonism and antihypertensive activity of phosphodiesterase inhibiting agents.


The behavioral effects of dibutyryl cyclic AMP in mice.


Estradiol-17 , cyclic AMP and prostaglandins: in vivo and in vitro studies on the cervicovaginal epithelium from neonatal mice.


Estrogens and the menopausal patient.

Estrogens and the menopausal patient. It is noted that advertisements in medical journals recommend treatment of emotional symptoms in menopausal patients with Premarin (Ayerst brand of conjugated estrogens), Ogen (Abbott brand of piperazine estrone sulfate), or other compounds.  There are no acceptable studies proving the usefulness of such combinations for symptoms relating to the menopause and no persuasive evidence to justify use of conjugated or any other type of estrogen in the treatment of emotional symptoms in  menopausal women.  Vasomotor symptoms, flushing, and sweats respond to estrogens.  Symptoms do not recur if treatment is stopped after 1 or 2 years.  Systematic or topical use of estrogens fails to promote the appearance of youthfulness.  Vaginal pruritus and dyspareunia due to atrophic vaginitis may be relieved by estrogens either applied locally or orally.  Libido is not heightened by exogenous estrogens but sufficient androgen doses cause virilization.  It is doubtful if osteoporosis is favorably influenced by long-term use of estrogens.  Estrogen therapy may cause spotting, menarrhagia, nausea, breast tenderness, or fluid retention.  Prolonged use may cause increase in size of uterine fibroids.  Personal or even family history of breast or genital cancer are considered contraindications.


Phosphodiesterase in tongue epithelium: activation by bitter taste stimuli.


Galactose metabolism and its regulation.


Subcellular distribution, age dependent variation and species differences of brain pyridoxal phosphate phosphatase.


Significance of clostridia in the female reproductive tract.


Gonadotropin producing hepatoma.

Gonadotropin producing hepatoma.


Effect of oral contraceptive therapy on the renin-angiotensin system in normotensive and hypertensive women. Plasma renin activity, plasma renin substrate (angiotensinogen), angiotensin I (AI), and plasma angiotensinase activity were measured in 8 women who had first become hypertensive during a mean 3.1 years on various oral contraceptives, and in  5 normotensive women, before and after 1 cycle of pills.  All were determined by radioimmunoassay.  8 or more blood pressures were taken by 2 observers and averaged.  Plasma renin activity increased from mean 2.12 to 3.52 ng/ml/hr (p greater than .2) in normal women, and from 3.0 to 5.06 in hypertensives (p less than .02).  The mean plasma renin substrate values for both groups together rose from 1881 ng/ml to 4245 ng/ml.  Angiotensin I rose from .029 ng/ml/hr to .049 in normals, and .027 to .037 in hypertensives.  Mean plasma angiotensinase activity values rose from 3.6 to 5.4% degraded per minute in normal women and varied only from 6.5 before and to 5.9 after a pill cycle in hypertensive patients.  The authors suggest that development of hypertension during oral contraceptive therapy may be due to abnormal inactivation of angiotensin.


Malignant mixed müllerian tumor of the uterine tube.


Granular cell myoblastoma of the vulva.

Granular cell myoblastoma of the vulva.


EEG patterns in relation to respiratory rate and body movement in Macaca mulatta.


Water and sodium chloride intake following microinjections of angiotensin II into the septal area of the rat brain.


Effect of adrenocorticotropic hormone on appetitive discrimination learning in the rat.


Instrumental learning without proprioceptive feedback.


Arousal from sleep: responsiveness to different auditory frequencies equated for loudness.


Viruses of the respiratory tract.

Viruses of the respiratory tract.


The programme of the World Health Organization in medical virology.


Advances in the serodiagnosis of viral infections.


Helper-dependent bacteriophage P4: a model satellite virus and its implications for animal virology.


Electron microscopy of viruses in thin sections of cells grown in culture.


Cytomegaloviruses of man and animals.

Cytomegaloviruses of man and animals.


Rectal ultrastructure in salt- and freshwater mosquito larvae in relation to physiological state.


Filament bundles and contractility of endothelial cells in coronary arteries.


On the regional metabolism of beef heart ventricles.


On the descending control of the lumbosacral spinal cord from the "mesencephalic locomotor region".


The ultrastructure of the sinuatrial ring bundle and of the caudal extension of the sinus node in the right atrium of the rabbit heart.


Estrogen for older women.

Estrogen for older women.


Some pathologic and biochemical aspects of Coats' disease.


Conservative management of congenital eversion of the eyelids.


The structure of a lipid-cytochrome c membrane. In the preceding paper a theory has been developed for the X-ray diffraction pattern from a lipid bilayer coated with protein molecules. In this paper the validity of the theory is tested. After adding cytochrome c to a preparation of lipid vesicles both vesicles and, in lesser amount, a multilayered structure are found. The expected cross-interference ripple is evident, and a computed profile shows the added protein at the surface of an unchanged lipid bilayer. The results confirm the structure proposed by others on the basis of rather indirect chemical and X-ray data. Protein coating a lipid bilayer is a possible structure for natural membranes. These results demonstrate that globular protein molecules can be detected at the surfaces of a lipid bilayer.


Striate-peristriate cortico-cortical connections in the albino and gray rat.


Patterns of degeneration after intrinsic lesions of the visual cortex (area 17) of the monkey.


Descending influences on spinocervical tract cell discharges evoked by non-myelinated cutaneous afferent nerve fibres.


Interactions of cutaneous myelinated (A) and non-myelinated (C) fibres on transmission through the spinocervical tract.


Effect of calcium and magnesium on spontaneous transmitter release accelerated by raised potassium.


Accumulation of adenosine 3',5'-monophosphate induced by catecholamines in the rat superior cervical ganglion in vitro.


Serum magnesium, serum cholesterol and serum lipoproteins in cases of coronary artery disease.


Some subcortical determinants of visual cortical excitability in the cat.


The effect of pertinent and non-pertinent stimuli on the secretions on thyrotropin and corticotropin.


Platelet aggregation in atopic and normal patients.


Effect of training on enzyme activity and fiber composition of human skeletal muscle.


A comparison of the major apolipoprotein from pig and human high density lipoproteins.


The purine nucleotide cycle. 3. Oscillations in metabolite concentrations during the operation of the cycle in muscle extracts.


Stimulation of steroid secretion by antimicrotubular agents.


Enzymes of pentose biosynthesis. II. Evidence that the proposed control of glucose 6-phosphate dehydrogenase by reduced diphosphopyridine nucleotide is an instrumental artifact.


Adenosine 3',5'-monophosphate in glial tumor cells. Alterations by 5-bromodeoxyuridine.


The mechanism of action of the flavoprotein melilotate hydroxylase.


The purification and properties of the flavoprotein melilotate hydroxylase.


Pituitary-adrenal interaction and growth of the embryonic avian adrenal gland.


Fibrous histiocytoma or xanthoma of the lung with bronchial involvement.


The role of specific IgM globulin estimations in the diagnosis of acquired rubella.


Systemic corticosteroid therapy.

Systemic corticosteroid therapy.


Characterization of chemically induced mutations in the ad-I locus of Schizosaccharomyces pombe.


Genetic analysis of purple adenine (ad-3) mutants induced by methyl methanesulfonate in Neurospora crassa.


Production of cyclic inositol phosphate in stimulated tissues.


Levels of cyclic AMP in sparse and dense cultures of growing and quiescent 3T3 cells.


Cyclic AMP and growth of fibroblasts: effect of environmental pH.


Cylindroma of the sphenoid sinus. A study of two cases.


Bovine virus diarrhea associated with severe pneumotropism.