Penetration and removal of horseradish peroxidase injected into the cerebrospinal fluid: role of cerebral perivascular spaces, endothelium and microglia.


Insulin secretion and glucose metabolism during succinylcholine paralysis in dogs.


Evolutionary divergence in the two kinds of subunits of ribulose diphosphate carboxylase isolated from different species of Nicotiana.


Analysis of high molecular weight material from the polymerization of adenosine cyclic 2', 3'-phosphate.


Simultaneous isolation of adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) in small tissue samples.


Flotation equilibrium of serum low density lipoproteins.


An enzymatic, spectrophotometric glycerol assay with increased basic sensitivity.


Adrenal-gonadal interactions at puberty.


Nuclear receptor-estrogen complex: in vivo and in vitro binding of estradiol and estriol as influenced by serum albumin.


Radioimmunoassay for aldosterone and normal values under various physiological conditions.


Steroid excretion in the neo-natal period: a comparative study of the excretion of steroids by human, ape and rhesus monkey infants.


Demonstration of an androgen binding protein (ABP) in rabbit testis: secretion in efferent duct fluid and passage into epididymis.


Influence of adrenal steroidogenesis in urinary excretion of catecholamine acid metabolites.


Specificity of cyclic AMP potentiation of glucose-stimulated insulin release.


Glucose transport in isolated brush-border and lateral-basal plasma-membrane vesicles from intestinal epithelial cells.


The effect of oxygen on the photoconductivity of lipid bilayers containing magnesium-porphyrin.


On the sidedness of plasma membrane enzymes.


Two variants of feedback artificially created in central motor disturbances on the base of EMG.


Photooxidative and spectral studies on cytochrome c. Conformational changes induced by binding of cardiolipin.


Characteristics of the sensory discharge of the muscle spindle in Xenopus laevis.


Modification of fructose-1,6-diphosphatase with pyridoxal 5'-phosphate. Evidence for the participation of lysyl residues at the active site.


Chemical polymerization of oligonucleotides directed by a complementary polynucleotide. Preparation and polymerization of oligo(2'-O-methylinosine 3'-phosphate).


Substrate and product inhibition in the xanthine oxidase catalyzed oxidation of acetaldehyde.


Neurospora arginyl transfer ribonucleic acid ligase. Binding and dissociation of transfer ribonucleic acid.


Contribution of carotenoids to the optical activity of human serum low-density lipoprotein.


Linear electric field effect measurements of variant low-spin forms of ferric cytochrome c.


Interactions of periodate-oxidized guanine nucleotides with Escherichia coli elongation factor G and the ribosome.


Chemical linkage of nucleic acids to neutral and phosphorylated cellulose powders and isolation of specific sequences by affinity chromatography.


The genetics of Caenorhabditis elegans.

The genetics of Caenorhabditis elegans. Methods are described for the isolation, complementation and mapping of mutants of Caenorhabditis elegans, a small free-living nematode worm. About 300 EMS-induced mutants affecting behavior and morphology have been characterized and about one hundred genes have been defined. Mutations in 77 of these alter the movement of the animal. Estimates of the induced mutation frequency of both the visible mutants and X chromosome lethals suggests that, just as in Drosophila, the genetic units in C. elegans are large.


Gel electrophoresis, isoelectric focusing, and localization of thymidine kinase in normal and simian virus 40-infected monkey cells.


Induction of common transplantation antigen by various isolates of papovavirus SV40 and by virus rescued from transformed cells.


Enzyme activities associated with cytoplasmic polyhedrosis virus from Bombyx mori. II. Comparative studies.


On the nature of the hemolytic activity of Sendai virus. A kinetic study.


Sanfilippo A disease in the fetus.

Sanfilippo A disease in the fetus.


Polysyndactyly and Marfan's syndrome.

Polysyndactyly and Marfan's syndrome.


E.s.r.-spectroscopy of radiation-produced radicals in 9-ethyl adenine.


Genetic variation in coumarin hydroxylase activity in the mouse (Mus musculus). Basal and phenobarbital-induced rates of hepatic metabolism of coumarin to 7-hydroxycoumarin are markedly higher in DBA/2J mice than they are in the AKR/J, C57BL/6J, and C3H/HeJ strains. Intermediate coumarin hydroxylase activity in F(1) hybrids of mating between DBA/2J and the other three strains indicates an additive mode of inheritance.


Insulin binding to isolated human adipocytes.


Increased sensitivity of cell-free protein synthesis to double-stranded RNA after interferon treatment.


Absence of REM rebound after barbiturate withdrawal. Administration of three different barbiturates reduced rapid eye movement (REM) sleep. Drug withdrawal led to a return to baseline REM] values without significant overshoot. Similar results are observed with administration of benzodiazepines in pharmacologically equivalent dosages; therefore, a distinction between these two drug classes on the basis of withdrawal effects on the sleep electroencephalogram appears unwarranted. Further investigation is required determine why high REM levels are sometimes associated with the withdrawal of sedative-hypnotic agents.


Partial suppression by UV irradiation of the mechanism of resistance to cucumber mosaic virus in a resistant cucumber cultivar.


Comparative studies on tomato aspermy and cucumber mosaic viruses. II. Virus stability.


Posttranscriptional block to adenovirus replication in nonpermissive monkey cells.


Rescue of simian virus 40 (SV 40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells.


Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus.


Influence of increasing 2'-O-methylation on the interferon stimulating capacity of poly(rl)-poly(rC).


Production of interferon-like substance by mouse spleen cells through contact with BHK cells persistently infected with HVJ.


Transcription of herpes simplex type 1 DNA in nuclei isolated from infected HEp-2 and KB cells.


Redetermination of the RNA content and the limiting RNA size of three strains of cucumber mosaic virus.


Synthesis of alpha-fetoprotein by membrane-bound polysomes of rat ascites hepatoma cells.


Cross-reacting tumor-associated antigen (s) among chemically induced rat colon carcinomas.


Deviation in esterase isozyme pattern during early stage of hepatocarcinogenesis by 3'-methyl-4-dimethylaminoazobenzene.


Virus release by cell colonies derived from chickens and cultures infected with avian myeloblastosis virus.


Effect of nitrofurans antagonistic to 3'-methyl-4-dimethylaminoazobenzene in hepatocarcinogenesis and RNA polymerase activity of liver cell nuclei in rats.


Proceedings: Proposed mechanisms by which autochthonous neoplasms escape immune rejection.


Proceedings: The graded enzymic immaturity of transplanted neoplasms.


Hormonal interactions among GH, ACTH, cortisol and dexamethasone upon size of kidney, liver, and adrenal.


Identification by immunofluorescence of adenoviral antigen in exfoliated bladder epithelial cells from patients with acute hemorrhagic cystitis.


Depressed growth of Morris hepatomas and altered lysosomal hydrolases during altitudinal hypoxia.


Interferon cell species specificity: role of cell membrane receptors.


Growth of Novikoff hepatoma cells in the presence of long-chain fatty acids.


Epidermal cell-leukocyte interactions in spongiform pustules of Kogoj. An ultrastructural study.


Progressive multifocal leukoencephalopathy with paramyxovirus-like structures, Hirano bodies and neurogibrillary tangles.


Morphological and biochemical observations in the Jimpy spinal cord.


Steroid therapy of multiple myeloma and macroglobulinemia.


The molecular basis of connective tissue pathology.


The structure of the gills of the elasmobranch, Scyliorhinus canicula (1).


Characteristics of aortic diastolic pressure decay with application to the continuous monitoring of changes in peripheral vascular resistance.


Copper content and exchange in mammalian hearts.


Effect of ischemia on capillary pressure and equivalent pore radius in capillaries of the isolated dog hind limb.


Lipoprotein uptake and metabolism by rat aortic smooth muscle cells in tissue culture.


Studies on the cardiomegaly of the spontaneously hypertensive rat.


Nerve and muscle stimulation of the rat isolated phrenic nerve-diaphragm preparation.


A Raman and infrared spectroscopic study of the 3-carbonyl group of pyridine nucleotide coenzymes and related model compounds.


Chemical studies on the cyanogen bromide peptides of rat skin collagen. Amino acid sequence of alpha 1-CB3.


Separation of ribonucleotides and deoxyribonucleotides on columns of borate covalently linked to cellulose. Application to the assay of ribonucleoside diphosphate reductase.


Study of free and bound lipids of Brassica campestris, var yellow sarson.


In vitro activity of delta 6 and delta 9 desaturases in hepatomas of different growth rates.


Unusual lipids. II. Head oil of the north atlantic pilot whale, Globicephala melaena melaena.


Hyperlipoproteinaemia in peripheral arterial disease in the north of England.


Enhancement by rat liver preparations of the gene conversion frequency induced by ethyl methanesulphonate in Saccharomyces cerevisiae.


Comparative studies of the effects of carcinogenic and antitumor agents on the DNA replication of cultured mammalian cells.


Effect of actinomycin D, cycloheximide and puromycin on hepatic adenosine 3',5'-monophosphate in rats treated with glucagon.


Electron-microscopic investigation of pathologic inclusions in neuron mitochondria during retrograde degeneration.


Lymphocytopoiesis after irradiation.

Lymphocytopoiesis after irradiation.


The pathology of colitis and other inflammatory diseases of the colon.


Neutrons in radiotherapy--yes or no?

Neutrons in radiotherapy--yes or no?


Biological effects of fast neutrons.

Biological effects of fast neutrons.


Compact cyclotron neutron dosimetry.

Compact cyclotron neutron dosimetry.


Experimental studies on the regulation of myocardial and adipose tissue lipoprotein lipase activitites in rat.


The electron paramagnetic resonance of oxidized clostridial ferredoxins.


Regulation of fructose, 1,6-bisphosphatase by histidine under gluconeogenic conditions. Fructose 1,6-bisphosphatase (EC 3.1.3.11) requires a free divalent metal and a metal chelate for optimum activity in the neutral pH range. The latter requirement can be satisfied by histidine, which appears to function as the chelating metabolite in vivo. Under fasting conditions, the concentrations of histidine in rabbit liver vary in the range required for activation of fructose bisphosphatase, and the enzyme itself is modified so that it becomes more responsive to histidine.


Pattern of immunoglobulin synthesis and assembly in a human-mouse somatic cell hybrid clone. Fusion of human peripheral blood lymphocytes, not forming detectable immunoglobulins, with mouse myeloma cells (TEPC-15), secreting mouse immunoglobulin A with known antibody activity, yielded a somatic cell hybrid clone that secreted both human and mouse immunoglobulins. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that human gamma, alpha, and light chains, as well as mouse alpha and light chains, were formed by the hybrid cells. To determine whether individual antibody molecules with both human and mouse components were secreted, medium from the hybrid cells was precipitated first with antibody against mouse immunoglobulin produced in rabbit, reduced, and alkylated, and then reprecipitated with antibody against human immunoglobulin produced in rabbit. Human gamma, alpha, and light chains were detected after electrophoresis of the immunoprecipitates on sodium dodecyl sulfate-polyacrylamide gels, indicating that antibody molecules containing both human and mouse components were secreted by the hybrid cells. These data indicate that this somatic cell hybrid clone synthesized human gamma and alpha heavy chains and human light chains, as well as mouse alpha heavy chains and mouse light chains. Some of these immunoglobulin components were assembled as hybrid antibody molecules.


A phosphate-acceptor protein related to parvalbumins in dogfish skeletal muscle. A phosphate-acceptor protein was isolated from the skeletal muscle of the Pacific dogfish (Squalus acanthias) displaying properties extremely similar to those of the parvalbumins, i.e., the low-molecular-weight, soluble, Ca-binding muscle proteins found in fish and amphibians. It has the same characteristic UV spectrum, strong affinity for calcium, and immunological crossreactivity with antibodies against homogeneous dogfish parvalbumin. Although it was isolated in three states of aggregation with molecular weights of about 350,000, 75,000, and 25,000, all species dissociate in Na dodecyl sulfate into subunits of 11,000 and 13,000 molecular weight. Furthermore, whereas no phosphorylation of parvalbumins could be demonstrated under any experimental conditions, the aggregated forms could be readily phosphorylated by a cyclic AMP-independent dogfish protein kinase, but not by phosphorylase kinase. One acid-stable and base-labile phosphate group was introduced per subunit which could be rapidly released by a dogfish protein phosphatase, but only very slowly if at all by phosphorylase phosphatase. It is speculated that this "phosphate-acceptor protein" might represent a physiologically active form of the parvalbumins.


Dominance of the senescent phenotype in heterokaryons between replicative and post-replicative human fibroblast-like cells. In heterokaryons between senescent and young diploid fibroblast-like cells, dominance of the former with respect to nuclear DNA synthesis (incorporation of [(3)H]thymidine) was demonstrated. For identification of the respective partners, double-layer autoradiography was used after the old cells were labeled with [(3)H]methionine and the young cells were labeled with [(14)C]thymidine. Synchrony of nuclear labeling (i.e., all nuclei in a cell labeled with [(3)H]thymidine) was observed in the majority of di- and polykaryons during the second and third of three 24-hr periods of labeling with [(3)H]thymidine. The results are compatible with either terminal differentiation or error theories of clonal senescence.


Localization of replicating DNA of simian virus 40 in monkey kidney cells. Approximately 80-85% of the DNA synthesized by cells infected with simian virus 40 during a short pulse with [(3)H]thymidine is associated with host nuclear membrane components, as defined by the M-band technique. A substantial portion of this membrane-associated DNA has been shown to be viral specific by sedimentation studies and by DNA.DNA hybridization. Examination of DNA associated and unassociated with nuclear M-band by neutral sucrose gradient analysis suggested that while replicating DNA of simian virus 40 is attached to the nuclear membrane, mature component I DNA of simian virus 40 is released from the membrane upon completion of replication.


Effects of dialyzable transfer factor in patients with breast cancer. Five patients with advanced breast cancer were treated with pooled dialyzable transfer factor from healthy adult donors. The period of treatment ranged from 21 to 310 days, the total dose from 20 to 257 ml. Transfer factor did not elicit inflammatory or hypersensitivity reactions or detectable formation of antibody to itself, nor any hematological or biochemical abnormalities or other side effects. Three patients became responsive (by skin test) to tuberculin and/or streptococcal antigens. Marked partial regression of the breast cancer, lasting 6 months, was observed in one patient.


Lipid binding to the amphipathic membrane protein cytochrome b5. The lipid binding properties of the membrane protein cytochrome b(5) (detergent-extracted from calf liver microsomal preparations) were characterized by studying the interaction of spin-labeled lipids (5-, 12-, and 16-doxylstearic acid and 5- and 16-doxylphosphatidyl-choline, where doxyl refers to the nitroxide moiety) with cytochrome b(5), using electron spin resonance spectroscopy. The intact cytochrome b(5) molecule immobilizes all of the lipid spin labels, while the segment of cytochrome b(5) released by trypsin does not affect lipid mobility. The immobilization of lipid spin labels on the hydrophobic surface of intact cytochrome b(5) is not appreciably altered by associating the protein with liposomes. Differences in polarity of the lipid binding sites between cytochrome b(5) and phospholipid vesicles were also observed. The lipid binding sites on cytochrome b(5) are hydrophobic by conventional criteria, but are more polar than the interior of fluid phospholipid bilayers.


Glucose inhibition of adenylate cyclase in intact cells of Escherichia coli B. Previous studies in E. coli B have demonstrated an inverse correlation between the presence of glucose in the medium and the accumulation of cyclic AMP in the medium. This observation could not be explained by the action of glucose as a repressor of adenylate cyclase (EC 4.6.1.1) synthesis, as a stabilizer of cyclic AMP phosphodiesterase (EC 3.1.4.17) activity, or as a direct inhibitor of adenylate cyclase activity in cell-free preparations. The recent development of an in vivo assay for adenylate cyclase has provided a basis for further exploring the inhibitory action of glucose in intact cells. With this assay it has been possible to show that, while glucose does not affect adenylate cyclase in vitro, it rapidly inhibits the enzyme activity in intact cells. Extensive metabolism of glucose is not required, since alpha-methylglucoside also inhibits adenylate cyclase in vivo. When cells are grown on glucose as carbon source, some sugars (mannose, glucosamine) substitute for glucose as adenylate cyclase inhibitors while others (e.g., fructose) do not. Dose-response studies indicate that low concentrations of glucose lead to essentially complete inhibition of adenylate cyclase activity while only moderately decreasing intracellular cyclic AMP concentrations. The evidence presented suggests that the decreased cellular cyclic AMP levels resulting from glucose addition can be accounted for by inhibition of adenylate cyclase without any significant effect on cyclic AMP phosphodiesterase or the transport of cyclic AMP from the cells to the medium.


Isolation of the major viral glycoprotein and a putative precursor from cells transformed by avian sarcoma viruses. Immune precipitation with a monospecific antiserum was employed to study the synthesis of the major viral glycoprotein gp85. Labeled gp85 was detectable by polyacrylamide gel electrophoresis of immune precipitates prepared from lysates of transformed cells which had been labeled for long term with radioactive amino acid or fucose. When immune precipitates were prepared from lysates of cells pulse-labeled with radioactive amino acid, the bulk of the precipitated counts did not appear in gp85 but in a heterogeneous protein fraction with a mean molecular weight of approximately 70,000; this fraction has been designated p70. If, however, the pulse label was followed by incubation of the cells in medium containing excess unlabeled amino acid, the bulk of the precipitated counts comigrated with gp85. Similar pulse-labeling experiments with radioactive fucose and glucosamine suggested that p70 represents incompletely glycosylated precursor to gp85.


The isolation and characterization of a new iron-sulfur protein from photosynthetic membranes. A new iron-sulfur protein, distinct from the soluble chloroplast ferredoxin, was isolated from chloroplast membranes. The isolated protein, purified to homogeneity, had a molecular weight of about 8000 and 4 atoms of iron and 4 inorganic sulfides per mole. Its absorption spectrum had a broad absorbance band in the 400 nm region, a shoulder at approximately 310 nm, and a peak around 280 nm. The absorbance ratio A(400) to A(280) was 0.55. The electron paramagnetic resonance spectrum (measured at 12 degrees K) of the reduced protein was similar to that of other reduced iron-sulfur proteins, showing a major resonance line at g = 1.94. The isolated protein, when photoreduced by spinach chloroplasts, can in turn transfer electrons to mammalian cytochrome c. However, the photoreduced protein cannot replace soluble ferredoxin in NADP(+) reduction because of its apparent inability to interact with the chloroplast enzyme, ferredoxin-NADP(+) reductase. The relation of the isolated iron-sulfur protein to the bound ferredoxin that acts as the primary electron acceptor in Photosystem I is discussed.


Regulation of adenylate kinase and creatine kinase activities in myogenic cells. The regulation of the specific activities of adenylate kinase (EC 2.7.4.3) and creatine kinase (EC 2.7.3.2) in myogenic cell lines is independent of cell fusion. The observed increases in enzyme specific activities are cell density dependent, and may be further broken down into contributions from an increase in enzyme activity per cell and a decrease in protein per cell. Only the former appears to be affected by medium conditioning.


The homopolyadenylate and adjacent nucleotides at the 3'-terminus of 30-40s RNA subunits in the genome of murine sarcoma-leukemia virus. Adenosine is the major 3'OH-terminal nucleoside of the 60-70S RNA genome of the murine sarcoma-leukemia virus, its 30-40S RNA subunits, and the poly(A) segments derived by RNase treatment of both RNA species, as determined by periodate oxidation-[(3)H]-borohydride reduction. The binding 30-40S RNA to oligo(dT)-cellulose suggests that most viral RNA subunits contain poly(A). The molecular weight of poly(A) derived from viral RNA by digestion with RNase and purified by affinity chromatography is 64,000-68,000, as determined by gel electrophoresis. From the size of poly(A) and the poly(A) content of viral RNA (1.6%), it is estimated that there is about one poly(A) segment for each viral 30-40S RNA subunit. The results of 3'-termini labeling with [(3)H]borohydride, in vivo labeling with [(3)H]adenosine, and base composition of [(32)P]poly(A) indicate that a homopoly(A) segment is located at the 3'-end of a 30-40S RNA subunit. The homogeneous poly(A) segments isolated from RNase T1 digests of 60-70S [(32)P]RNA consist of one cytidylate, one uridylate, and about 190 adenylate residues, while those isolated from RNase A digests consist exclusively of adenylate residues. These results indicate that -G(C,U)A(190)A(OH) is the 3'-terminal nucleotide sequence of the viral 30-40S RNA subunits.


Induction of a lactogenic receptor in rat liver: influence of estrogen and the pituitary. A receptor exists in female rat liver with high specificity for lactogenic hormones. Previous work showed the receptor level increased at the time of puberty in female but not male animals. Pregnancy caused a further substantial increase. Here we show that estrone (50 mug/day) administration to male rats induced a 10- to 30-fold increase in specific binding of ovine prolactin and human growth hormone after 8-12 days with a significant increase first seen after 4 but not 2 days of injection. In females, this regimen increased binding to pregnancy levels. In prepuberal (20-days-old) male and female rats, estrone was also markedly stimulatory. The binding sites for ovine prolactin and human growth hormone were of high affinity in liver membranes from both female and estrone-treated male rats (K(a) = 0.6 to 1.4 x 10(9) M(-1)). Estrone and estradiol were equally effective in inducing the lactogenic receptor. Estriol (50 mug/day), progesterone (500 mug/day), human placental lactogen (1 mg/day), and testosterone (100 mug/day) were without influence. Hypophysectomy drastically decreased the levels of lactogenic receptor in mature female rats, and estrogen treatment failed to restore receptor levels to normal. Hypophysectomized male rats were also unresponsive to estrogen. Throughout these studies the specific binding of (125)I-labeled insulin remained relatively constant. This work demonstrates estrogen induction of a lactogenic receptor. The pituitary gland appears to have a critical, though presently undefined, role in the induction process.


Short-term binding and hydrolysis of cyclic 3':5'-adenosine monophosphate by aggregating Dictyostelium cells. Transient binding of cyclic AMP to aggregating cells of Dictyostelium discoideum was measured under cyclic GMP excess in order to inhibit cyclic AMP hydrolysis by cell-bound phosphodiesterase. Cyclic GMP extended the period of half-maximal cyclic AMP binding from less than 5 sec to 1-2 min. With the same time course as bound cyclic AMP was released from the cells, labeled 5'-AMP appeared in the medium. Specificity, kinetics, and developmental regulation suggest that the cyclic AMP-binding sites exposed in living cells are identical with receptor sites for the chemotactic response. The functioning of the cyclic AMP-receptor/phosphodiesterase system and its formal similarity with the synaptic acetylcholine-receptor/esterase system are discussed.


Effect of poliovirus double-stranded RNA on viral and host-cell protein synthesis. Cell-free protein-synthesizing systems that initiate on endogenous messenger RNA have been developed from uninfected and poliovirus-infected HeLa cells. Poliovirus double-stranded RNA is an effective inhibitor of protein synthesis in these extracts, and both cell-directed and virus-specific protein synthesis are equally sensitive to the inhibitory action of double-stranded RNA. The concentrations of double-stranded RNA required for inhibition are not achieved in the infected cell at early times after infection when host-cell shut-off occurs, but rather are achieved only late in infection when virus-specific protein synthesis begins to decline. This indicates that double-stranded RNA does not act as a direct agent to inhibit host cell protein synthesis following infection by poliovirus. The possible significance of inhibition by double-stranded RNA of poliovirus-specific protein synthesis is discussed.


Kinetic properties and electron paramagnetic resonance spectra of the nitric oxide derivative of hemoglobin components of trout (Salmo irideus). The binding of nitric oxide to hemoglobin components of trout (Salmo irideus), i.e., Hb trout I and Hb trout IV, has been studied by optical and electron paramagnetic resonance spectroscopy. Kinetic studies show that the Root effect in Hb trout IV is operative also for NO, since a large increase in the dissociation velocity constant (j(4)) is observed as the pH is decreased below 7. Moreover, the time course of the displacement of NO by CO is heterogeneous, suggesting that alpha and beta chains may have different j(4) values. Low-temperature X-band electron paramagnetic resonance spectra have been recorded with Hb trout I and IV saturated with NO at different pH values. The spectra of Hb trout IV are strongly pH-dependent. The high-pH form (pH 8.1) shows axial symmetry and no resolved hyperfine splitting, while the low-pH form is rhombic with a hyperfine splitting of 6.5 G in the g(z) region. The latter form reflects a more distorted site with a more significant delocalization of the unpaired electron on the proximal histidine; both features indicate a destabilization of the ligand binding at low pH. On the other hand, spectra of Hb trout I are axial at both pH values, with hyperfine splitting of 16.5 G, indicating that the site is not distorted and interacts with the ligand very strongly at either pH.


Phosphate metabolism in intact human erythrocytes: determination by phosphorus-31 nuclear magnetic resonance spectroscopy. Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to decrease in association with a concomitant increase in inorganic orthophosphate. When aged cells containing little 2,3-diphosphoglycerate were incubated in the presence of inosine and pyruvate, 2,3-diphosphoglycerate formation could be demonstrated. These results show that cellular metabolism can be recorded directly in intact cells by (31)P nuclear magnetic resonance.


Demonstration of dopamine-sensitive adenylate cyclase in malignant neuroblastoma cells and change in sensitivity of adenylate cyclase to catecholamines in "differentiated" cells. Adenylate cyclase (EC 4.6.1.1) activity was stimulated by low concentrations of dopamine and apomorphine, but not by low concentrations of norepinephrine in homogenates of malignant mouse neuroblastoma cells. In cyclic AMP-induced "differentiated" cells, dopamine concentration required for a maximal increase in adenylate cyclase activity was about 10-fold less than that required for a similar increase in control cells, and norepinephrine-sensitive adenylate cyclase activity became apparent at low norepinephrine concentrations. The pharmacological properties of dopamine-sensitive adenylate cyclase were different from those of norepinephrine-sensitive enzyme. For example, dopamine-stimulated adenylate cyclase activity was markedly reduced by low concentrations of haloperidol and phentolamine, whereas norepinephrine-stimulated enzyme activity required higher concentrations of these blocking agents for a similar amount of inhibition. Norepinephrine-stimulated enzyme activity was markedly blocked by low concentrations of propranolol, whereas dopamine-stimulated enzyme activity required a much higher concentration of this blocking agent for a similar amount of inhibition. Low concentrations of isoproterenol increased adenylate cyclase activity in malignant cells, but in "differentiated" cells even a high concentration failed to do so. The fact that dopamine and norepinephrine produced an additive stimulatory effect on adenylate cyclase activity suggests that they interact at different receptor sites. This suggestion is further supported by the observation that the combination of prostaglandin E(1) and norepinephrine produced an additive stimulatory effect of enzyme activity. The observation that the effects of dopamine and prostaglandin E(1) are not additive, coupled with the observation that a low concentration of phentolamine blocked the effect of prostaglandin E(1), suggests that these two agents may interact at a common site.


The mechanism of viral replication. Structure of replication complexes of encephalomyocarditis virus. The structure of the purified replicative intermediate of encephalomyocarditis virus was determined by electron microscopy. Approximately 80% of the replicative intermediate complexes were characterized by a filament of double-stranded RNA of widely variable length, which had a "bush" of single-stranded RNA at one end. In many examples one or more additional single-stranded bushes were appended internally to the double-stranded RNA filament. These results support the view that before deproteinization, replicative intermediate contains little if any double-stranded RNA.


Cholera toxin mimics melanocyte stimulating hormone in inducing differentiation in melanoma cells. Cholera toxin (choleragen) and melanocyte stimulating hormone alter within hours the morphology of melanoma cells in culture, and they slow the growth of serum-stimulated cells. After 7-10 days, cells exposed to choleragen or hormone show increased size and a fibroblastic growth pattern. Tyrosinase (EC 1.14.18.1; monophenol monooxygenase) activity increases after 3 days in the presence of 10(-8) M hormone or 10(-10) M choleragen. Binding studies with (125)I-labeled choleragen indicate that although a melanoma cell can bind a maximum of 10(6) molecules of cholera toxin, only about 4000 binding sites must be occupied to achieve maximum stimulation of tyrosinase activity. Melanocyte stimulating hormone and choleragen probably have different membrane-binding sites. After exposure to choleragen for 5 min, membrane adenylate cyclase (EC 4.6.1.1) activity increases dramatically upon further incubation of intact cells for several hours at 37 degrees and falls slowly to basal values over a period of more than 10 days. Hormone stimulation of adenylate cyclase is rapidly reversed by washing the cells, but subsequent restimulation of cyclase by the hormone is impaired. These studies indicate that cAMP mediates the effects of melanocyte stimulating hormone on growth and morphology as well as on tyrosinase activity. Cholera toxin may permanently activate the available adenylate cyclase molecules, and the protracted decay of stimulation that follows may reflect the biological turnover of adenylate cyclase molecules in these cells.


Acquirement of fetal properties in hepatoma on glutathione metabolism.


Hormonal regulation of hepatic and jejunal formiminotransferase activity in man and rat.


Correlations between the enzymatic activities and the factors active on blood coagulation and platelet aggregation from the venom of Vipera aspis.


SV40 nucleoprotein complex activity unwinds superhelical turns in SV40 DNA.


Toxins of Vibrio cholerae and Escherichia coli stimulate adenyl cyclase in rat fat cells.


Binding of concanavalin A and ricin to synaptic junctions of rat brain.


Constitutivity of the HCG-receptor protein in the testis of rat and man.


Release of cyclic AMP from rat superior cervical ganglia after stimulation of synthesis in vitro.


The effect of angiotensin upon the vasculature of the isolated perfused rat kidney.


Meiotic drive in tn-bearing mouse spermatozoa: a relationship between aerobic respiration and transmission frequency.


The somatomedins: a family of insulinlike hormones under growth hormone control.


MSH, cyclic AMP, and melanocyte differentiation.


Interpreting the failures to confirm the depression of cerebellar Purkinje cells by cyclic AMP.


D(--)-lactic acid and d(--)-lactate dehydrohgenase in octopus spermatozoa. The spermatozoa of Octopus dofleini martini produce anaerobically D(-)-lactic acid and possess a very active D(-)-lactate dehydrogenase. In this respect, while resembling certain microorganisms, they differ strikingly from mammalian spermatozoa which produce L(+)-lactic acid and contain L(+)-lactate dehydrogenase.


The spectrum of antibacterial activity of human amniotic fluid determined by scanning electron microscopy.


Serum estradiol-17beta and serum human chorionic gonadotropin in patients with hydatidiform moles.


Progesterone concentrations in the sera of patients with intact and aborted hydatidiform moles.


Quantitation of the lecithin-sphingomyelin ratio in amniotic fluid by a simple charring method.


Respiratory movements in fetal rhesus monkeys.


Studies on the luteinizing hormone- and follicle-stimulating hormone-releasing mechanism in the testicular feminization syndrome. Hypothalamic-pituitary responsiveness to clomiphene, luteinizing hormone-releasing hormone, gonadectomy, and sexual steroids.


Viral teratogenesis: a review with experimental and clinical perspectives.


Cyclic AMP, ATP and cell contact.

Cyclic AMP, ATP and cell contact.


Correlations between plasma ACTH concentrations and breathing movements in foetal sheep.


Herpes simplex viruses: discrimination of types and correlation between different characteristics.


Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses.


The nucleotide sequence of chicken 5S ribosomal RNA.


Potentiated acid 1,5 pentanedial solution--a new chemical sterilizing and disinfecting agent.


Zonal electrophoresis and isoelectric focusing of proteins and virus particles in density gradients of small volume.


Analysis of hydroxyproline and hydroxylysine by gas chromatography.


The steroid specificity of the estrogen-receptor of human breast carcinoma.


Quantitative histologic distribution of adenosine-3',5'-monophosphate in the rat stomach. Improved procedure for the luminescence assay.


Mechanism of estrogen action--independence of several responses of the rat uterus from the early increase in adenosine 3',5'-cyclic monophosphate.


Involvement of the anticodon region of Escherichia coli tRNAGln and tRNAGlu in the specific interaction with cognate aminoacyl-tRNA synthetase. Alteration of the 2-thiouridine derivatives located in the anticodon of the tRNAs by BrCN or sulfur deprivation.


Nucleases in HeLa cell nucleoplasm and nucleoli.


Ribosomal RNA homologies in flowering plants: comparison of the nucleotide sequences in 5.8-S rRNA from broad bean, dwarf bean, tomato, sunflower and rye.


Lateral phase separations in Escherichia coli membranes.


Tumors of the submandibular gland.

Tumors of the submandibular gland.


Structural differences in the adenylate kinase enzymes.


Activity of microsomal enzymes in the livers of rats fed rapeseed oil.


Pathology of ulcerogenic and diarrheogenic tumors of the pancreas.


The effect of partial degradation of mitochondrial phospholipids by phospholipase A on the temperature dependence of succinate-cytochrome c reductase and cytochrome c oxidase.


Studies on the stability of rat liver D-glucose-6-phosphate phosphohydrolase (EC 3.1.3.9).


Uptake and accumulation of purine bases by stationary yeast cells pretreated with glucose.


Partitioning turkey blood plasma and egg yolk lipoproteins using agarose gel.


Regulation of membrane lipid fluidity in Acholeplasma laidlawii: effect of carotenoid pigment content.


Hematological responses associated with resistance to Rous sarcomas in four strains of chickens.


Chemistry of technetium 99m.

Chemistry of technetium 99m.


Effect of caffeine on the neuromuscular junction of the frog, and its relation to external calcium concentration.


Analysis of the relationship between 99mTc-Sn-polyphosphate and 99mTc-Sn-pyrophosphate.


A new electrophoretic technique for mouse, human, and Chinese hamster galactokinase.


The diagnostic value of bronchial washing in lung cancer.


Intraocular and optic nerve sheath hemorrhage in cases of sudden intracranial hypertension.


Blood loss and factors affecting pulmonary antibacterial defenses.


Estradiol mustard (NSC-112259) and phenester (NSC-116785): possible mediation of action by estrogen binding protein.


Ganglion of the peroneal nerve. Report of two cases.


The Mongolian gerbil as a model for lead toxicity. I. Studies of acute poisoning. Mongolian gerbils fed diets containing lead acetate maintained body weight comparable to gerbils fed the same diet without added lead. Intranuclear lead inclusion bodies in epithelial cells of the proximal convoluted tubules of the kidney were first observed at 4 weeks, and increased in number to about 50 per high power field at 12 weeks. At this time, a corticomedullary area of empty-appearing tubules was prominent. Transmission electron microscopy confirmed the increase in number and size of nuclear lead inclusion over the 12-week period. Cytoplasmic changes observed in proximal tubule cells containing lead inclusions were considered indicative of acute lethal injury. Distinct cytoplasmic fibrillar structures, first apparent at 8 weeks, were present in some proximal tubular lining cells and strongly resembled newly formed intranuclear lead inclusions. After 12 weeks, the total amount of lead present in the gerbil kidney was four to six times greater than that in rat kidney as determined by atomic absorption spectrophotometry. A hypothesis has been formulated that relates the more efficient nephron of the gerbil kidney to the rapid and extensive development of intranuclear inclusion bodies and the greater accumulation of total lead.


Some effects of 5-bromodeoxyuridine on polyoma-transformed mouse cells.


Induction of alkaline phosphatase activity by dibutyryl adenosine 3':5'-cyclic monophosphate in aneuploid rat liver cells.


Ultrastructural localization of SV40 viral DNA in cells, during lytic infection, by in situ molecular hybridization.


Ribosomal protein differences between animal cells.


A hospital's support systems for chronic patients living in the community.


Holding tea groups: a home visiting program for chronic schizophrenics.


Boerum Hill: a private long-term residential program for former mental patients.


A diseases of Sokoto goats resembling "peste des petits ruminants".


Inhibitory effects in the cuneate nucleus produced by vago-aortic afferent fibers.


Disynaptic corticospinal effects in forelimb motoneurones in the cat.


Effect of prolonged alcohol administration on calcium transport in heart muscle of the dog.


Perthes-like changes in the syndrome of caudal regression.


Ochratoxin A, an inhibitor of mitochondrial transport systems.


Virus encephalitis: pathogenesis in the immature brain.


Some chemical aspects of dose-response relationships in alkylation mutagenesis.


Caffeine-induced enhancement of chromosome damage in human lymphocytes treated with methyl-methanesulphonate, mitomycin C and X-rays.


Cytomegalovirus infection and sudden death in infants.


Early eruption of deciduous and permanent teeth: a case report.


A modified gas chromatographic method for lactate analysis.


Identification of a vasopressin-neurophysin and of an oxytocin-neurophysin in man.


Effects of neurogenic and systemic stresses on hypothalamic and adenohypophysial cAMP content.


Platelet glucose-6-phosphatase activity in patients with von Gierke's disease and their parents.


Lecithin:cholesterol acyltransferase and plasma proteins in liver diseases.


The binding of indocyanine green and other organic anions to serum proteins in liver diseases.


Investigations on the oncogenic activity for the chick embryo of ribonucleic acid (RNA) extracted from fowl sarcoma induced by the Carr strain (Zilber) of Rous virus.


Association between production of infectious virus and chromosomal aberrations in carcinomatous human cells (HeLa) persistently infected with Papova virus SV40.


Mitochondrial functions under hypoxic conditions. The steady states of cytochrome c reduction and of energy metabolism.


The effect of temperature on the primary reaction of chloroplast photosystem II. Evidence for a temperature-dependent back reaction.


Identification of ubiquinone as the secondary electron acceptor in the photosynthetic apparatus of Chromatium vinosum.


Light activation of phosphodiesterase activity in retinal rod outer segments.


EPR studies by 57Fe isotopic substitution on the nature of an unknown electron acceptor in Azotobacter vinelandii phosphorylating particles.


The synthesis of 3-deoxy-4-O-methyl-D-arabino-2-heptulosonic acid and its behaviour in the Warren reaction.


Studies on dextrans and dextranases. XI. The structure of a dextran elaborated by Leuconostoc mesenteroides NRRL B-1299.


A synthesis of (plus or minus)-myo-inositol 1-phosphate.


Alkaline sucrose gradient sedimentation studies of DNA from HeLa S3 cells exposed to methyl methanesulphonate or methylazoxymethanol acetate.


The determination of metallic elements in wastes and waters with the graphite furnace.


Using primates in medical reseach. 2. Recent comparative research. Cancer--a general review.


Using primates in medical research. 2. Recent comparative research. Communicable diseases: hazards for man and models for research.


Monkey cell cultures in virology.

Monkey cell cultures in virology.


Smallpox and monkeypox in primates.

Smallpox and monkeypox in primates.


Using primates in medical research. 1. Husbandry and technology. New World monkeys.


Trophic functions of the neuron. VI. Other trophic systems. Neurotrophic regulation at the neuromuscular junction.


Trophic interactions in the mammalian central nervous system.


Brain and meningeal infections in children. Radiological considerations.


Some properties of embryonic mitochondria of the loach.


The relationship between chromosomal aberrations, survival and DNA repair in tumour cell lines of differential sensitivity to X-rays and sulphur mustard.


Persistence of Adenovirus 5 in guinea pigs. One intracardiac inoculation of adenovirus 5 in guinea pigs leads to virus persistence in different organs, viz., 5 days in lungs and liver, 14 days in blood and lymph nodes, and 56 days or more in the spleen. After cultivation of tissue cells for 1 week, virus was recovered from blood, lymph nodes, or spleen lymphocytes, but virus could be detected directly in cells only when organs were removed within 48 h of inoculation. To determine how the virus persisted in low concentrations and as a latent infection, spleens were primarily selected for study by three techniques: homogenization of spleens, suspended Maitland fragment cultures, and in vitro cultivation of spleen cells. The last procedure showed virus in fibroblast-like cells (probably macrophages or reticuloendothelial cells) for 56 days after infection of guinea pigs. With other methods, the virus was found only within the first 2 days after inoculation.


Biomolecules from HCN.

Biomolecules from HCN.


Reactivity of envelope, capsid, and soluble antigens of Herpesvirus hominis types 1 and 2 in the indirect hemagglutination test. Envelope, capsid, and soluble antigens of Herpesvirus hominis (HVH) types 1 and 2 were compared to crude antigens (disrupted HVH-infected cells) for potency, type-specificity, and diagnostic value in the indirect hemagglutination (IHA) test. The envelope appeared to be the predominant component reacting in the IHA test, but recurrent HVH infections increased the reactivity of human sera with capsid antigens. The soluble antigens reacted in the IHA test with HVH immune animal sera, but very few convalescent-phase human sera showed reactivity with soluble antigens. Overall, none of the subunit antigens showed greater type-specificity than did crude antigens in IHA tests with immune animal or convalescent-phase human sera. Recurrent infections with type 1 or type 2 viruses tended to broaden heterotypic reactivity of the patients' sera with both crude and subunit antigens, even in patients showing only a single type of antibody by IHA inhibition. Subunit antigens were no more sensitive than crude antigens in demonstrating significant IHA antibody titer rises for serodiagnosis of herpesvirus infections, and they generally had to be used at lower working dilutions than crude antigens.


In vitro cellular immunity to unrelated pathogens in chickens infected with fowlpox virus. Peritoneal macrophages recovered from chickens 15 to 20 days after inoculation with fowlpox virus and showing a delayed hypersensitivity reaction against fowlpox antigens demonstrated an enhanced antimicrobial effect against fowlpox virus as well as unrelated viruses and bacteria. Inoculation of normal chicken macrophage cultures with fowlpox virus resulted in approximately a 200-fold increase in virus titer by 96 h, whereas the virus showed less than a fourfold increase in macrophage cultures from fowlpox-immune chickens. Similarly, the titer of Newcastle disease virus increased by more than 1 log in cultures of normal macrophages, whereas the titer decreased by approximately 1 log after infection of fowlpox-immune macrophages. Vaccinia, vesicular stomatitis, and herpes simplex viruses, which are not natural pathogens of chickens, failed to replicate in cultures of either normal or fowlpox-immune macrophages. By using Salmonella gallinarum, it was further demonstrated that the antimicrobial activity of fowlpox-immune macrophages encompassed not only nonspecific viruses but also bacteria. Infection of normal macrophages with Salmonella resulted in intracellular replication of the organism between 0 and 24 h as determined by microscope examination and viable bacteria counts. In contrast, cultures of fowlpox-immune macrophages failed to show an increase in intracellular organisms and showed a marked decrease in viable bacteria. In conclusion, these studies clearly showed that cellular immunity, previously demonstrated in mammalian species, develops in chickens after infection with fowlpox virus.


Production of chemotactic factor and lymphotoxin by human leukocytes stimulated with Herpes simplex virus. Peripheral blood leukocytes (PBL) of 41 subjects were tested for their ability to be stimulated by herpes simplex virus antigens as measured by [(3)H]thymidine incorporation and lymphokine production (i.e., lymphocyte-derived chemotactic factor and lymphotoxin). The PBL of seronegative subjects failed to respond to viral antigens as measured by stimulation or lymphokine production. In contrast, PBL from seropositive subjects without clinical lesions of herpes labialis were stimulated by herpes simplex virus antigens and produced lymphokines. PBL from seropositive patients with clinical lesions of herpes labialis also produced lymphokines, but [(3)H]thymidine incorporation was slightly depressed. These findings suggest that lymphocytes from patients with a recent herpes simplex virus infection may respond less vigorously to in vitro stimulation by herpes antigens, but our study fails to show any basic defect in the responsiveness of the lymphocytes to account for recurrent herpetic episodes.


Aspects of the geologic history of seawater.


Oxygen uptake by Treponema pallidum.

Oxygen uptake by Treponema pallidum. Virulent Treponema pallidum has been shown to consume O(2) at a rate similar to that of the known aerobic spirochaete, Leptospira. Such O(2) uptake is cyanide sensitive, indicating a functioning cytochrome oxidase. Inhibition of O(2) uptake by azide, chlorpromazine, and amytal further suggests a functioning electron transport system for the oxidation of nicotinamide adenine dinucleotide (reduced) to O(2). Evidence is consistent with the probability that this terminal electron-transport system is coupled to oxidative phosphorylation. The potential significance of these findings is discussed.


Escherichia coli enterotoxin-induced steroidogenesis in cultured adrenal tumor cells. A heat-labile, Pronase-sensitive factor has been partially purified from cell-free culture filtrates of enterotoxigenic Escherichia coli. The partially purified factor contains both protein and carbohydrate moieties and appears to be E. coli enterotoxin (ECT). ECT binds to cultured adrenal tumor cells rapidly and irreversibly leads to adenosine 3', 5'-cyclic monophosphate formation and steroidogenesis after a 60-min lag phase. Further studies indicate that it interacts with the cholera toxin receptor site on adrenal cells rather than the adrenocorticotropin receptor to activate adenyl cyclase. Mixed gangliosides block stimulation of steroidogenesis in response to both E. coli and cholera enterotoxin. In contrast to adrenocorticotropin, ECT has no additive effect on cholera toxin-induced steroidogenesis. The protein moiety of ECT is similar to cholera enterotoxin because horse serum anticholeragenoid prevented stimulation of steroidogenesis by either enterotoxin. Cultured adrenal cells provide a quantitative assay system that has facilitated the purification and characterization of E. coli enterotoxin.


Growth of murine cytomegalovirus in a heterologous cell system and its enhancement by 5-iodo-2'-deoxyuridine. Mouse cytomegalovirus replicated in rabbit kidney cultures, a cell system of nonrodent origin. However, the sensitivity of these cultures, and the yields of virus therefrom, were lower than those of mouse cultures. Although a cytopathic effect developed in rabbit kidney cultures inoculated with sufficient amounts of the virus, such cultures were unsatisfactory for plaque assay. This was also true when rabbit fibroblast cultures were used, even though the murine cytomegalovirus replicated much better in mouse fibroblasts than in mouse kidney cultures, the latter of which contained extensive areas of epithelial cells. Viral growth in rabbit kidney cells was considerably enhanced when those cells had been initiated and grown in the presence of 5-iodo-2'-deoxyuridine; not only were the viral titers increased, but also the clarity and distinctness of the inclusion bodies.


Comparison of techniques and immunoreagents used for indirect immunofluorescence and immunoperoxidase identification of enteroviruses. Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of the immunoperoxidase technique appear to be related to the relative size, charge, and uniformity of the preparations. Monomers of peroxidase-conjugated globulin are larger than those of FITC conjugates, but the latter readily form aggregates. This was shown by chromatography on Sepharose 6B columns: various FITC-conjugated globulins eluted before those conjugated to peroxidase and before (125)I-labeled immunoglobulin G. The net charge of the conjugates was determined by adsorption to ion-exchange columns. FITC-labeled globulins had a negative net charge, eluting at pH 5.1 from diethylamino-ethyl-Sephadex A-50 columns. Peroxidase conjugates were not retained by either cationic or anionic exchangers at pH values ranging from 4.0 to 10.5. Further, the fluorescein/protein ratios of FITC conjugates from different commercial sources and of those prepared in the laboratory were found to be variable; higher fluorescein/protein ratios (>2:1) give a higher degree of nonspecific reactions, whereas the peroxidase/protein ratio does not appear to affect specificity. These characteristics of peroxidase conjugates make the immunoperoxidase technique easier to standardize and more reliable for enterovirus identification than the immunofluorescence technique.


Origin and biological role of molecular asymmetry.


Editorial: Tempest in the chickenpot.

Editorial: Tempest in the chickenpot.


Benign tumors of the hand.

Benign tumors of the hand.


Molecular mechanisms of temperature compensation in poikilotherms.


Fetal hamartoma of the kidney--a benign tumour to be distinguished from Wilms' tumour.


Complementation of adeno-associated satellite viral antigens and infectious DNA by temperature-sensitive mutants of herpes simplex virus.


The host DNA sequences in different populations of serially passaged SV40.


Studies on action mechanisms of a possible false cholinergic transmitter, (2-hydroxyethyl) methyldiethylammonium.


A pilgrim's progress in cancer research, 1918 to 1974: autobiographical essay.


Blood glucose levels and gluconeogenesis in animals bearing transplantable tumors.


The presence of the Epstein-Barr viral genome in human lymphoblastoid B-cell lines and its absence in a myeloma cell line.


Alveolar cell carcinoma-like antigen and antibodies in patients with alveolar cell carcinoma and other cancers.


Mitochondrial protein content and enzyme activity of Reuber hepatoma H-351.


Inhibition of subcutaneously growing line 1 carcinomas due to metastatic spread.


Proceedings: Heterogeneity of carcinoembryonic antigen.


Estrogen excretion in a case of theca-granulosa cell tumor.


Prolactin binding to rat mammary tumor tissue.


Anterolateral hypothalamic deafferentation prevents compensatory hypersecretion of ACTH following adrenalectomy in the rat.


Isolation and characterization of a neutral deoxyribonuclease from the testes of the crab Cancer pagurus.


Different expressions of cooperativity in the kinetics of two forms of cytoplasmic malic dehydrogenase.


Fabry's disease: specific inclusions found on electron microscopy of fibroblast cultures.


Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.


DNA-synthesis in the spleen of mice after whole-body X-irradiation and its modification by 5-hydroxytryptamine and NADH.


Radical yield in X-irradiated biological molecules. II. Naturally-occurring amino-acids.


5'-Adenylylation and 5'-uridylation of N-acetyl tyrosine ethyl ester: a general synthesis of 5'-nucleotidylated tyrosyl residues.


Metabolism of glycol-derived lipids in Novikoff hepatomas.


Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.


The length of repetitive sequences of monkey cell and salmon sperm DNA.


Decreased basal cyclic adenosine 3',5'-monophosphate levels in Morris hepatoma 5123 t.c. (h).


TSH regulation of cAMP-dependent protein kinase activity in the thyroid.


Stimulation of DNA polymerase activity by the combination of p-hydroxymercuribenzoate and dithiothreitol.


A proposed hydrogen transfer function for cytochrome c. Outlines of proposed mechanisms for cytochrome c oxidoreduction are presented which differ in detail from those previously published. The mechanisms, while accommodating the fact that cytochrome c is a net electron acceptor and donor in its catalytic cycle, dictate that the enzyme catalyses hydrogen transfer. It is shown that the mechanism of cytochrome c reduction fulfills a recent proposal that the transition state is an analogue of the structure of ferricytochrome c(2). The implications of a hydrogen transfer role of cytochrome c are discussed.


Structure-function correlations of formylmethionine transfer RNA.


Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. I. Mannans produced in phosphate-containing medium.


Direct measurement of electric current generation by cytochrome oxidase, H+-ATPase and bacteriorhodopsin.


Preferential inhibition of the growth of virus-transformed cells in culture by rifazone-82, a new rifamycin derivative. Rifazone-8(2), a new rifamycin derivative, is shown to preferentially inhibit the growth of virus-transformed chick cells in culture. Macromolecular synthesis and glucose uptake of transformed cells are also appreciably decreased in the presence of low concentrations of rifazone-8(2) where the normal cells appear unaffected. While rifazone-8(2) is shown to be a selective inhibitor of RNA-directed DNA polymerase in vitro, its action on the growth of transformed cells may involve some other mechanism.


Effects of nucleotides, hormones, ions, and 1,25-dihydroxycholecalciferon on 1,25-dihydroxycholecalciferol production in isolated chick renal tubules.


Preparation of cell lysate for separation of polyoma DNA from cellular DNA by sedimentation velocity.


Maintenance of pregnancy in rats on deficient diets.


Virus-augmented tumor transplantation antigens: evidence for a Helper antigen mechanism.


Reduced transplantability of syngeneic tumors in rats immunized with allogeneic tumors.


Occupational lung cancer among copper smelters.


Induction of neurogenic tumors in C3HeB-FeJ mice by nitrosourea derivatives: observations by light microscopy, tissue culture, and electron microscopy.


Frequency dependence of dual negative feedback control of secretion of sympathetic neurotransmitter in guinea-pig vas deferens. The secretion of sympathetic neurotransmitter in guinea-pig vas deferens appears to be normally controlled by two different mechanisms, one dependent and one independent of prostaglandin E. The efficiency of both control systems, as well as of exogenous prostaglandin E(2), is inversely related to nerve stimulation frequency.


Basal cell adenoma of intraoral minor salivary gland origin.


Inhibition by diuretics of cyclic 3',5'-AMP-dependent protein kinase from toad bladder epithelium. 1. A cAMP-dependent protein kinase enzyme has been isolated from toad bladder epithelium.2. This enzyme catalyses the phosphorylation of histones; a reaction stimulated by 0.1 muM cAMP.3. Activity of this enzyme in the presence of 0.1 muM cAMP was significantly inhibited by 100 muM mercuderamide, bumetanide and frusemide. A small, though statistically insignificant inhibition was seen with the same concentrations of hydrochlorothiazide and ethacrynic acid.4. The half maximal inhibition was achieved with mercuderamide, 5 muM, and with frusemide and bumetanide 600 muM.


The oncogenicity of Epstein-Barr virus.

The oncogenicity of Epstein-Barr virus.


Bronchoscopic diagnosis of cytomegalovirus infection.


Studies on 1-beta-D-arabinofuranosyl-cytosine (Ara-C) resistant mutants of Chinese hamster fibroblasts. II. High resistance to Ara-C as a genetic marker for cellular hybridization.


Cell surface interactions with concanavalin A. Location of bound radiolabeled lectin.


A differential chemotactic response of slime mould amoebae to regions of the early amphibian embryo.


Serological studies of normal and leukemic cats in a multiple-case leukemia cluster.


Low resistance junctions between normal and between virus transformed fibroblasts in tissue culture.


Herpesviruses and cervical cancer.

Herpesviruses and cervical cancer.


Perspectives on the epidemiology of cervical cancer with special reference to the herpesvirus hypothesis. Epidemiological considerations with regard to mortality statistics and incidence rates for cervical cancer are discussed and recent data are summarized.  The unresolved problem of the nature of the relationship between cervical dysplasia and carcinoma in situ with invasive cervical cancer is considered.  A summary is made of data on time trends in the incidence of cervical cancer and of the details of studies on the biological behavior of cervical carcinoma in situ.  Coital/marital and penile factors in the etiology of cervical cancer are reviewed, and the herpesvirus hypothesis is evaluated in light of methodological considerations.  Finally, a major new collaborative prospective test of the herpesvirus hypothesis is proposed.


Epidemiological studies relating genital herpetic infection to cervical carcinoma. Epidemiological studies relating genital herpetic infection to cervical carcinoma are reviewed.  The high frequency of herpes simplex virus type 2 (HSV-2) antibodies in young women (21 years or younger) with cervical carcinoma in situ and in women with dysplasia or carcinoma in situ, matched for various sexual attributes to control women, provide support for a causal relation.  However, various laboratory, histopathological, and statistical problems associated with all epidemiological studies do not yet permit a firm conclusion as to the etiological role to the genital virus in cervical carcinogenesis.  With the use of herpes-related cancer antigens or purified HSV-2 type-specific antigens, and with the possible development of protective HSV-2 vaccines, the application of epidemiological approaches may be necessary to provide the most finite evidence of causality.


Biochemical approaches to detection of herpes simplex virus type 2 in cervical carcinoma.


A nonhuman primate model for the study of the cervical oncogenic potential of herpes simplex virus type 2.


Discussion of case-control study of Hodgkin's disease.


Nasopharyngeal carcinoma: recent studies and outlook for a viral etiology.


Antibodies to herpes group viruses in patients with nasopharyngeal and other head and neck cancers.


Antibody levels to infectious agents other than Epstein-Barr virus in Burkitt's lymphoma patients.


Immunological reactions to tumor-associated antigens in Burkitts lymphoma and other lymphomas.


Animal models: herpesvirus saimiri, a nonhuman primate model for herpesvirus-associated neoplasia of man.


Epstein-Barr virus DNA in Hodgkin's disease, American Burkitt's lymphoma, and other human tumors.


Epstein-Barr virus: one or a family of viruses.


DNA and nucleoprotein changes in liver and tumor nuclei after irradiation.


Eicosatrienoic acid omega9 in serum lipids of patients with hepatocellular carcinoma.


Studies on the dense pre-beta-lipoprotein of human serum.


3,4-Methylene hexanedioic acid--a previously unknown compound in human urine.


Direct contact between a neuron and soma and myocardial cells; an electronmicroscopic finding in the sinus venosus of the turtle heart.


Antiviral compounds. XVI. p-Alkylthiophenylglyoxals and their derivatives.


Cell culture of the endocrine pancreas of the mouse in chemically defined media.


Femoral cortical erosions and osteoarthrosis of the knee with chondrocalcinosis. An anatomo-radiological study of two cases.


The effect of drugs influencing the level of cyclic AMP on angiotensin hyperglycaemia in rabbits.


Clindamycin phosphate in severe infections mainly caused by Bacteroides fragilis: clinical and microbiological evaluation.


Seroepidemiology of herpesvirus type Epstein-Barr in blood donors from communities around Lyon.


Occurrence of respiratory illness due to an atypical strain of adenovirus type 11 during a large outbreak in Spanish military recruits.


Study of Japanese encephalitis virus in Chiangmia Valley, Thailand. V. Animal infections.


Study of Japanese encephalitis virus in Chiangmia Valley, Thailand. VI. Summary and conclusions.


Use of the delayed brain scan in differentiating calvarial from cerebral lesions.


Further studies on chemical closure of the Fallopian tubes in the monkey.


A fluorometric kinetic method for creatine in urine.


Purification and further characterization of three DNA polymerases of rat Ascites hepatoma cells.


Effect of protein deficiency on the inducibility of the hepatic microsomal drug-metabolizing enzyme system. 3.


Inhibition of 2-deoxy-D-glucose transport by theophylline, caffeine and papaverine in alveolar macrophages.


Antibody formation after irradiation and its cellular background.


Anti-tumor activity in regression fluid.


Endocrine action during insect growth.

Endocrine action during insect growth.


Nerve growth factor and insulin: evidence of similarities in structure, function, and mechanism of action.


The lysosome as a mediator of hormone action.


Microtubule proteins in the nerve growth factor mediated response. Interaction between the nerve growth factor and its target cells.


Insulin secreting pancreatic adenoma demonstrated by selective angiography.


Heterogeneity of peptide hormones.

Heterogeneity of peptide hormones.


Preparation of DNA-splitting activities yielding 3'-mononucleotides from the sponge Verongia aerophoba.


Adenocarcinoma of the lung cytologic correlation with histologic types.


Cellular changes in the glandular epithelium of patients using IUCD--a source of cytologic error.


Hydrophobic binding sites in bovine serum albumin and erythrocyte ghost proteins. Study by spin-labelling, paramagnetic fluorescence quenching and chemical modification.


Biological properties of two strains of Simian virus 40 isolated from patients with progressive multifocal leukoencephalopathy. Biological properties of two strains of simian virus 40 (SV40) from brains of two patients with progressive multifocal leukoencephalopathy (PML) have been compared to those of a standard laboratory strain of SV40. Infectivity of both SV40-PML viruses was resistant to treatment with chloroform, low pH, and 50 C for 120 min. African green monkey kidney and BSC-1 cells were the most sensitive for viral replication, and cytopathology in these cultures was indistinguishable from that caused by SV40. Both viruses formed plaques in these cells. but, in African green monkey kidney cells, strain 1 virus produced plaques measuring 2 mm in diameter whereas strain 2 virus produced pleomorphic plaques varying from 1 to 10 mm in diameter. Hamster cells were not permissive for viral replication, and infection resulted only in viral transformation. Inoculation of human fetal glial cells resulted in a permissive lytic infection of one cell type and a persistent infection with only partial expression of the viral genome in the other. No morphological evidence of transformation was evident in the latter cells. Both strains of SV40-PML viruses were neutralized by commercial anti-SV40 serum, but in reciprocal kinetic neutralization tests differences in K values were noted when each was compared to SV40. Both viruses showed oncogenicity for hamsters, producing undifferentiated sarcomas when injected subcutaneously and choroid plexus papillomas after intracerebral inoculation. All hamster tumor cells contained intranuclear immunofluorescent tumor antigen. This was indistinguishable from SV40 T antigen in reciprocal staining reactions using hamster anti-T antibody induced by the two SV40-PML agents and SV40. These two human agents appear therefore to be new variants of simian virus 40.


Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. The immunoflourescent antibody technique (IFAT) and cell culture isolation procedures were compared for their efficiency in the etiological diagnosis of viral respiratory illness in children. Before the IFAT was incorporated as a routine procedure, antisera used in the test were carefully calibrated to insure specificity. A study was then conducted in which 375 nasopharyngeal suctions were investigated by both IFAT and isolation for the presence of parainfluenza virus types 1, 2, and 3, respiratory syncytial, influenza A, and influenza B viruses. Methods already established in our hospital for patient management and specimen collection were not altered for the purposes of the study. The IFAT, as conventionally practiced in the detection of respiratory virus antigens, requires adequate numbers of ciliated epithelial cells. There were 68.5% specimens which contained cells suitable for IFAT, whereas 31.5% had either an insufficient number or inappropriate types of cells and could be used only for virus isolation. Cell-associated immunoglobulins were detected in 16% of those specimens with adequate cells. When all specimens were considered regardless of their cell population, IFAT was inferior to isolation in diagnostic efficiency. However, isolation complemented by IFAT resulted in a statistically significant increase in number of positive virus identifications. Under routine working conditions in a large pediatric hospital, it was found that IFAT could not replace isolation techniques but could, if used in conjunction with isolation, provide a significant overall increase in number of positive diagnoses. The time that the specimen was taken in relation to first symptoms was found to be an important variable with respect to the method most likely to succeed in virus identification.


Hepatocellular localization of fetal antigens during induction of rat liver tumors by 3'-methyl-4-dimethylaminoazobenzene.


Primary neoplasms in dog liver induced by diethylnitrosamine.


Biosynthesis of globoside and Forssman-related glycosphingolipid in mouse adrenal Y-1 tumor cells.


The effects of di-butyryl cAMP on enzymatic and metabolic changes in explants of rat mammary tissue.


Influence of reserpine-induced depletion of noradrenaline on the negative feed-back mechanism for transmitter release during nerve stimulation. 1. The effects of depletion of endogenous noradrenaline by reserpine-pretreatment on [(3)H]-noradrenaline overflow elicited by nerve stimulation were determined in the isolated nerve-muscle preparation of the cat's nictitating membrane.2. Reserpine pretreatment (0.3 mg/kg, s.c., 4 days prior to the experiment) reduced the noradrenaline levels in the smooth muscle of the nictitating membrane to about 10% of the control values while granular retention of [(3)H]-noradrenaline had recovered to nearly 40% of the controls.3. In the reserpine-pretreated tissue the fraction release per shock induced by nerve stimulation was 2.2-fold higher than the value obtained in the untreated tissues. This effect was correlated with the degree of depletion of the noradrenaline stores rather than with the decrease in the response of the effector organ.4. Phenoxybenzamine, 2.9 muM reduced the responses to nerve stimulation to the same extent in control and in reserpine-pretreated tissues. Yet, this concentration of phenoxybenzamine increased by 13-fold the overflow of the labelled transmitter in the controls and only by 3-fold in reserpine-pretreated tissues.5. The decrease in effectiveness of phenoxybenzamine in enhancing transmitter overflow after reserpine-pretreatment appears to be due to the decrease in the total release of the transmitter.6. The results obtained support the view that in reserpine-pretreated tissues decreased transmitter output reduces the activation of the presynaptic alpha-adrenoceptors which mediate the negative feed-back mechanism that regulates transmitter release by nerve stimulation.


Presynaptic and postsynaptic effects of the venom of the Australian tiger snake at the neuromuscular junction. 1. Crude venom (TSV) from the Australian tiger snake (Notechis scutatus scutatus) has both presynaptic and postsynaptic effects at the neuromuscular junctions of toads.2. TSV (50 mug/ml) rapidly blocked indirectly elicited muscle twitches without affecting the compound action potential in the sciatic nerve or twitches elicited by direct stimulation.3. Low concentrations of the venom (1-10 mug/ml) reduced the amplitude of miniature endplate potentials (m.e.p.ps) and inhibited the depolarization of muscle fibres normally caused by carbachol. It was concluded that a fraction of the venom binds to acetylcholine receptors.4. The frequency of m.e.p.ps was at first increased by TSV at a concentration of 1 mug/ml. Occasional, high frequency ;bursts' of m.e.p.ps were recorded in some preparations. The mean frequency of m.e.p.ps appeared to fall after several hours in the venom.5. The quantal content of endplate potentials (e.p.ps) was reduced by the venom. With low concentrations (1 mug/ml), an initial increase in quantal content was often seen. When the quantal content was markedly depressed there was no parallel reduction in the amplitude of nerve terminal spikes recorded extracellularly, though a later fall in size and slowing of time course was often seen.6. There was evidence that TSV eventually changed the normal Poisson characteristics of the spontaneous release of quanta and this may be correlated with electronmicroscopic changes in nerve terminals.7. Tiger snake antivenene counteracted the postsynaptic, but not the presynaptic effects of TSV when they had developed.


Transformation of chick embryo neuroretinal cells by Rous sarcoma virus in vitro: induction of cell proliferation. Neuroretinal cells from 7-day-old chick embryos are transformed and induced to proliferate after infection with Rous sarcoma virus in vitro. Susceptibility of neuroretinal cells to the virus is also dependent on the stage of development since infection of cells from 10-day-old embryos is uneffective.


Management of burn syndactyly.

Management of burn syndactyly.


Degranulation of sensitised rat peritoneal mast cells in response to antigen, compound 48-80 and polymyxin B. A scanning electron microscope study.


The distribution of 5-bromodeoxyuridine in the DNA of polyoma-transformed mouse cells and some apparent effects on transcription.


Studies on a murine form of spontaneous lower motor neuron degeneration--the wobbler (wa) mouse. The wobbler (wr) mouse has been considered an animal model of spontaneous lower motor neuron degeneration which is inherited as an autosomal recessive trait. Previously available data are, however, also compatible with genetic factors playing a necessary though not exclusive role in the expression of the wobbler trait. The present report describes the results of chromosome analyses, electromyography and selected histochemical studies on wobbler mice, as well as studies exploring the possible participation of infectious agents, transneuronal effects, retrograde effects, aging, circulating toxins and immunologic factors in the pathogenesis of the wobbler disorder. The data obtained do not implicate any of these mechanisms in the pathogenesis of the wobbler trait and also indicate that the wobbler mouse is not a clinical variant of the recently described type C oncornavirus-induced murine paralytic disease.


Chemotactic responses of human polymorphonuclear leukocytes to cyclic GMP and other compounds.


Infectious pericarditis in children.

Infectious pericarditis in children.


Muscular hypertrophy in hypothyroid children: the Kocher-Debré-Semelaigne syndrome. A review of 23 cases.


Postural protection against complications in radiculography with dimer X.


Type-specific tumor patterns in the combined early and late cerebral scintigrams.


The binding of 1alpha-hydroxycorticosterone to tissue soluble proteins in the skate, Raja ocellata.


Trophic functions of the neuron. VI. Other trophic systems. Taste bud regeneration in organ culture.


Studies on fish liver protein synthesis. I. Isolation and characterization of shark liver transfer ribonucleic acid.


Development of host-mediated mutagenicity tests. I. Differential response of yeast cells injected into testes of rats and peritoneum of mice and rats to mutagens.


Mutagenicity of hycanthone in Drosophila melanogaster.


Assessment of biological age. Methodological study in two finnish populations.


Monosynaptic projections from the pontine reticular formation to the 3rd nucleus in the cat.


Observations on pathogenic organisms in the airborne state.


Presynaptic modulation of la fiber synaptic effectiveness without concurrent postsynaptic inhibitory conductances.


Ultrastructural aspects of the liver in cases of constitutional non haemolytic hyperbilirubinemia before and after treatment with barbiturates. Morphological aspects of enzymatic induction.


Canine glomeruli: light and electron microscopic change in biopsy, perfused, and in situ autolyzed kidney from normal dogs.


A contribution to the study on embryonal lung cancer (alveogonioma).


A review of prostaglandin F2alpha for ovulation control in cows and mares.


Persistence and expression of the herpes simplex virus type 2 genome in cervical tumor cells.


Nasopharyngeal carcinoma: present status of knowledge.


Transformation of nonhuman primate lymphocytes by Epstein-Barr virus.


Uranyl acetate as an excellent fixative for lipoproteins after electrophoresis on agarose gel.


Formation of high-energy phosphate bonds effected by electron-deficient sulfides.


Newer beta-lactam antibiotics.

Newer beta-lactam antibiotics.


Meningeal and glial tumors in combination.


Antineoplastic substances from the sea: a review.


Cross-reacting tumor-associated antigen(s) of adenovirus type 9-induced fibroadenomas and a chemically induced mammary carcinoma in rats.


Enhanced Na+-K+-activated adenosine triphosphatase activity in transformed fibroblasts.


Interrelation of afferent impluses from different bellies of the temporal muscle of the cat.


The immunologic modulation of infectious disease pathogenesis. Friedenwald Lecture, 1973.


Radioimmunoassay for mouse mammary tumor virus-associated antigen.


Klinefelter syndrome and cancer. A family study.


Pathways for glucose metabolism in the rat gingiva. I. Patterns of enzymes of glucose metabolism.


Association of Wolff-Parkinson-White syndrome with congenital abnormalities of hands and feet.


Electroneurography in intermittent claudication due to obliterative arteriosclerosis.


A sporadic case of apparent Crouzon's syndrome with extracraniofacial manifestations.


Association of PPi-phosphohydrolytic activity with fractions of alkaline orthophosphatase (EC 3.1.3.1) from human placenta.


Isolation of infectious bovine rhinotracheitis virus from feces of a feeder steer.


Epipolythiopiperazinedione antibiotics from Penicillium turbatum.


Suggestive evidence for an oncorna-virus-specific DNA polymerase from C-type particles of bovine leukosis.


Influence of sodium and cyclic AMP on the passive permeability of the mucosal membrane in the rat intestine.


The lysosomal enzymes in the regression of the mesonephron. Histochemical observations on chick embryo development.


The resident's page: pathologic quiz case 2.


Adenoid cystic carcinoma in the head and neck (cylindroma).


Three dimensional structure of adenyl kinase.


Comparison of predicted and experimentally determined secondary structure of adenyl kinase.


Topological comparison of adenyl kinase with other proteins.


Food vacuole membrane in nutrient uptake by Tetrahymena.


The reaction of xanthine oxidase with molecular oxygen.


The mechanism of action of xanthine oxidase.


Heme-spin label studies of hemoglobin. I. Preparation and properties of heme-spin-labeled ferrihemoglobin.


Effects of temperature on conformation, hydroxylation, and secretion of chick tendon procollagen.


Effect of sulfate on the activity and the kinetics of dexoyribonucleic acid degradation by porcine spleen deoxyribonuclease.


The mechanism of action of ethanolamine ammonia-lyase, a B-12-dependent enzyme. The participation of paramagnetic species in the catalytic deamination of 2-aminopropanol.


Interactions of saccharides with concanavalin A. Relation between calcium ions and the binding of saccharides to concanavalin A.


The recovery of anaerobic bacteria from swabs. When a standard sample of simulated exudate containing known numbers of anaerobic bacteria was taken up on a swab and plated on solid medium, the number of colonies subsequently cultured represented a very small proportion of the original sample. Evidence is produced that the apparent loss is not primarily attributable to inactivation on the swab but rather to retention of organisms on the swab. This was demonstrable with Clostridium welchii and with Bacteroides species that have hitherto been regarded as relatively oxygen-sensitive.When stock strains of Bacteroides species were held for some hours on swabs, some progressive loss of viability was demonstrable. A measure of protection was afforded when these organisms were held aerobically on blood agar medium, but a very exacting anaerobe and some wild strains of faecal anaerobes showed gradual inactivation under these conditions.These findings may have important implications in relation to currently employed bacteriological sampling procedures with swabs in clinical practice.


Selective binding of human interferon to albumin immoblized on agarose.


A study of foot-and-mouth disease virus strains by complement fixation. I. A model for the fixation of complement by antigen-antibody mixtures. An examination was made of the relations between antigen, antibody and fixation of complement with foot-and-mouth disease virus (FMDV). It was found that complement fixation in this system follows the same principles as models developed in other antigen/antibody systems. The assumption that there is a relation of direct proportionality between the amount of complement fixed and the amount of antiserum reacting with constant antigen was found to be incorrect. An alternative method was proposed for the quantitative differentiation of FMDV strains by comparing the titres of an antiserum when reacting with optimum amounts of homologous or heterologous antigens.


A study of foot-and-mouth disease virus strains by complement fixation. II. A comparison of tube and microplate tests for the differentiation of strains. Several foot-and-mouth disease virus strains were examined by complement-fixation tests in microplates and in tubes. It was established that the two systems are comparable, although greater reproducibility is obtained with tube tests. While microplate tests are a satisfactory method for the differentiation of strains, tube tests provide a more precise method for the identification of small antigenic differences.


Viruses associated with acute respiratory infections 1961-71. Influenza-like illness, cold and sore throat was the diagnosis given in over 80% of 5177 acute respiratory illnesses in patients swabbed over a 10-year-period. A pathogenic organism was isolated twice as frequently from patients with a sore throat or an influenza-like illness as from those diagnosed as suffering from croup or laryngitis and bronchitis. A laboratory diagnosis was commoner in school children than in older or younger persons.Most of the organisms isolated were found in association with all types of acute respiratory illness but, with increasing age of the patient, one particular agent or group of agents was more likely to be of aetiological significance.


Diagnosis of idiopathic hypertrophic subaortic stenosis by right ventricular septal biopsy.


Antibodies against adeno-, cytomegalo and rubella viruses in Australia-antigen-negative sera from patients with infectious hepatitis. Antibodies neutralizing adenovirus type 5 were found in all of 50 pairs (100%) of sera from patients with acute icteric infectious hepatitis. The incidence in sera from the general population was 57%. No differences in mean titre or in proportion of positive sera were found in the same sera tested for complement-fixing antibodies to cytomegalovirus and for antibodies to rubella virus haemagglutinin. The results can be interpreted as supporting the involvement, either direct or indirect, of adenovirus in the aetiology of infectious hepatitis; but could also be due to a non-specific anamnestic enhancement of the production of antibody to adenovirus, or to coincidental infection with adenovirus and the agent of infectious hepatitis.


The Brenner tumor and the Walthard cell nest. An electron microscopic study.


The Golgi complex. II. The effects of aminonucleoside on ultrastructure and glycoprotein biosynthesis.


Ultrastructure of the parafollicular (C) cells and the parathyroid cell in growing dogs on a high calcium diet.


Evaluation of a new mucolytic drug.

Evaluation of a new mucolytic drug.


Pharmacologic effects of nicotinic acid on human purine metabolism.


Spontaneous eyelid behavior in the sleeping cat.


Effects of glutamate on spontaneous electrical activity in the embryonic chick cerebrum.


Rigidity and primary motoneuron damage in tetanus.


The uptake process for gamma-aminobutyric acid in cultures of developing chick cerebrum.


Superinfection with adenovirus of Burkitt's lymphoma cell lines.


Effects of partial hepatectomy on carcinogenicity, metabolism, and binding to DNA of ethyl carbamate.


Isolation and characterization of a cell line from the cocultivation of Lucké renal tumor cells and nontransformed feeder cells.


Cell culture from a rat brain tumor induced by intracerebral inoculation with murine sarcoma virus.


Effect of cyclopropenoid compounds on the carcinogenic activity of diethylnitrosamine and aflatoxin B1 in rats.


Neuraminidase and cell viability: failure to detect cytotoxic effects with dye-exclusion techniques.


In vivo inhibition of murine leukemia and sarcoma viruses by the heteropolyanion 5-tungsto-2-antimoniate.


Suppression of chemically induced pulmonary tumors by treatment of strain A mice with murine sarcoma virus.


Ultrastructural studies of cell-virus interaction in reptilian cell lines. II. Distribution, incidence, and factors enhancing the production of intramitochondrial virions.


Destruction of syngeneic tumors by tuberculin-stimulated peritoneal exudate cells from guinea pigs immunized to Mycobacterium bovis (strain BCG).


The murine male antigen. 3. Variable expression of H-Y by murine tumors.


Induction of adenofibrosis and hepatomas of the liver in BALB-cJ mice by polychlorinated biphenyls (Aroclor 1254).


Several changes associated with the acquisition of a single chromosome in rat glial tumor cells.


Treatment of metastatic Wilm's tumor.

Treatment of metastatic Wilm's tumor.


Editorial: The "oat-cell" tumors: a medical and biological challenge.


Drug levels in the target tissue and effect.


The supine subcostal modification of the thoraco-abdominal incision.


Blood level measurements and antimicrobial agents.


Zinc deficiency and decreased pancreatic secretory response.


Peritubular membrane transport of PAH into proximal cells in Necturus kidney slices.


Comparison of the effects of cholera enterotoxin and ACTH on adrenal cells in tissue culture.


Pulmonary adema and hemorrhage as a consequence of systemic vasoconstriction.


Effect of pH on epinephrine-induced lipolysis in isolated fat cells.


Influence of sodium, potassium, and pituitary hormones on iso-renin in rat adrenal glands.


Computer-assisted analysis of chromosomal abnormalities: detection of a deletion in aniridia-Wilms' tumor syndrome. A chromosome translocation, t(8p + ; 11q -), in a patient with aniridia and Wilms' tumor, appeared balanced by standard techniques, including trypsin banding. Computer analysis of optical microscope scanning profiles of chromosome pairs 8 and 11 revealed an interstitial deletion of the short arm of 8. Computer analysis coupled to the new banding techniques provides greater resolution for the detection of subtle chromosomal variations not recognized by banding methods alone.


Cancer of the pancreas induced in the Syrian golden hamster. A high incidence of pancreatic neoplasms was induced in Syrian golden hamsters following subcutaneous applications of diisopropanolnitrosamine (DIPN) once weekly for life. The tumor latency was as short as 15 weeks. In clinical and morphologic aspects the induced pancreatic tumors closely resembled those of humans.


Toxic polyneuropathy produced by methyl N-butyl ketone. A polyneuropathy affecting a large number of workers was recently observed at a plant producing plastic-coated and color-printed fabrics. Epidemiological data suggested strongly that methyl N-butyl ketone (MBK) was responsible for the outbreak. This hypothesis is now supported by the development of a peripheral neuropathy in chickens, rats, and cats exposed to MBK at atmospheric concentrations of 200 to 600 parts per million, 24 hours per day, 7 days per week. Although the animals were exposed continuously and the affected workers were exposed intermittently, the averages of the total number of hours of exposure for development of the peripheral neutropathy in the animals and workers were remarkably close.


Animal model of human disease. Burkitt's lymphoma, malignant lymphoma and lymphocytic leukemia.


Altering lipid patterns in adult males.

Altering lipid patterns in adult males.


Reduced calcium absorption after nephrectomy in uremic patients.


The renin-angiotensin system (second of two parts).


Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 34-1974.


Cellular pathology and uterine cancer. Ward Burdick Award address.


Effects of actinomycin D on RNA metabolism in the growing hair follicles of the mouse.


Autoimmunity and neoplasia. The possible role of C-type viruses.


Carcinoma of the gallbladder and extrahepatic biliary tree.


Macrodactyly and localized hypertrophic neuropathy.


The innervation of muscle spindles in a case of hypertrophic polyneuropathy.


Ocular myasthenia gravis mimicking progressive external ophthalmoplegia. Rare case of myasthenia associated with peripheral neuropathy and spastic paraparesis.


The fate of kidney transplants from donors with minor renal abnormalities.


Trace elements in noraml and malignant human breast tissue.


Letter: Effect of exogenous oestrogens on minor psychiatric symptoms in postmenopausal women.


Prevention of food poisoning in hospital patients.


Oestrogen assays in gynaecology.

Oestrogen assays in gynaecology. The applications of total urinary estrogen and pregnanediol assays in nonpregnant patients are presented.  Estrogen levels provide information on overall ovarian activity, and when measured periodically during the ovulatory cycle, give values of 10-20 mcg/24 hours during the first week, 45-100 mcg/24 hours just before ovulation, then back down and  up again to a second maximum of 30-100 mcg/24 hours during the luteal phase.  Urinary estrogen levels are useful in determining whether ovarian function is present or absent, since values remain less than 10 mcg/24 hours in complete absence of ovarian activity.  Anovulatory cycles are also characterized by low pregnanediol levels.  These assays are also important in determining proper dosage for gonadotrophin and clomiphene treatment.  Both assays are useful in the differentiation between disease and normal pregnancy.


The preparation of microsomal fractions of rodent respiratory tract and their characterization.


Enhancement of 7,12-dimethylbenzanthracene skin carcinogenesis by adenosine 3',5'-cyclic monophosphate.


A comparative study of rat liver, muscle, and hepatoma 3924A phosphofructokinase isozymes.


Synthesis, toxicities, and carcinogenicities of carcinogenic bifunctional aminoazo dyes.


Endogenous type C particles from rat embryo cells treated with 5-bromodeoxyuridine.


Isozyme patterns of pyruvate kinase in various primary liver tumors induced during the process of hepatocarcinogenesis.


Rabbit antibodies to nucleoli of Novikoff hepatoma and normal liver of the rat.


Substrate requirements and kinetic analysis of the cortisol effects on uridine uptake and incorporation by mouse lymphoma P1798 cells in vitro.


Polyamine depletion of tumor tissue and subsequent elevation of spermidine in the sera of rats with 3924A hepatomas after 5-fluorouracil administration.


Stromal nephromas and renal cell tumors in suckling and weaned rats.


Transfer RNA methylase activity in benign human ovarian neoplasms.


Overview on pathogenesis of cardiac hypertrophy.


Massive hemothorax: two unusual cases.

Massive hemothorax: two unusual cases.


Travelers' warning: dipyrone in over-the-counter drugs.


Bilateral symmetrical metastases to the clavicular heads from bronchogenic carcinoma simulating primary hyperparathyroidism.


Proceedings: Electron spin resonance study of the role of calcium in the fibrinogen fibrin reaction.


The role of transferrin in iron transport.


A temperature-sensitive mutant of herpes simplex virus type 1 defective in the synthesis of the major capsid polypeptide.


Inhibition of foot-and-mouth disease virus replicase by frog virus 3 particles.


The induction of deoxythymidine kinase by bacteriophage T4.


Electron microscopic observations on the entry of influenza virus into susceptible cells.


Transformation of rat embryo cells by temperature-sensitive mutants of herpes simplex virus.


Inactivation of some bacterial and animal viruses by exposure to liquid-air interfaces.


The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG.


Absence of a requirement for host polypeptides in the herpes virus thymidine kinase.


Treatment of gestational trophoblastic disease.


A clinical test useful for predicting the development of acute hypertension in pregnancy.


Relative importance of viral and neoantigens in cytotoxic reaction against murine leukaemia cells.


Simple siliceous pneumoconiosis in Negev Bedouins.


The inhibition of mouse bone collagenase by lysozyme.


Vibrational spectra of some phosphate salts amorphous to X-ray diffraction.


The lipids of matrix vesicles from bovine fetal epiphyseal cartilage.


The effect of galactose on bone metabolism.


Vitamin A deficiency stimulated phosphatase activity in epiphyseal chick cartilage.


Cytochemical, ultrastructural, and autoradiographic study of the intranuclear crystals in the midgut cells of Gyrinus marinus Gyll.


Human serum albumin phenotype activation in mouse hepatoma--human leukocyte cell hybrids. Murine hepatoma cells that secreted mouse serum albumin were fused with human leukocytes that did not produce albumin. The resulting hybrids secreted both mouse and human serum albumin, as demonstrated by immuno-electrophoretic techniques. The activation of the human genome suggests that mapping genes governing specialized functions in somatic cell hybrids may be accomplished by using adifferentiated human cells as a parental line.


Antibody to leukemia virus: widespread occurrence in inbred mice. Mice from a wide variety of inbred strains produce immunoglobulin G antibody against murine leukemia virus. This is contrary to the common view that the mouse is immunologically tolerant to its endogenous leukemia virus.


Cooperative effects of AMP, ATP and fructose 1,6-P2 on the specific elution of fructose 1,6-diphosphatase from cellulose phosphate.


Affinity chromatography. New approaches for the preparation of spacer containing derivatives and for specific isolation of peptides.


The biosynthesis of riboflavin: affinity chromatography purification of GTP-ring-opening enzyme.


Immobilized polynucleotides and nucleic acids.


Preparation, characterization and applications of enzymes immobilized on inorganic supports.


Immobilized cofactors and multi-step enzyme-systems.


The preparation of microenvironments for bound enzymes by solid phase peptide synthesis.


The optimization of porous materials for immobilized enzyme (IME) systems.


Chain refolding and subunit interactions in enzyme molecules covalently bound to a solid matrix.


Use of immobilized enzymes for synthetic purposes.


Studies on the site of origin of the androgen binding protein present in epididymal cytosol from mature intact rabbits.


Androgen binding protein (ABP) in rabbit testis and epididymis.


In vitro cellular immunity to Epstein-Barr virus in normal human subjects.


Disappearance of a major cell-type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by Rous sarcoma virus.


Attempts to detect virus-secific DNA in human tumors. I. Nucleic acid hybridizations with complementary RNA of human wart virus.


An analysis of variables affecting the measurement of tumor immunity in vitro with 125I-iododeoxyuridine-labelled target cells. Studies of immunity to primary Moloney sarcomas.


Attempts to detect virus-specific DNA in human tumors. II. Nucleic acid hybridizations with complementary RNA of human herpes group viruses.


Proceedings: Influence of large doses of rifampicin on mammalian embryonic development.


Cell-mediated and humoral immunity studies of murine and hamster sarcoma virus-induced producer and non-producer tumors in syngeneic animals.


Proceedings: The effect of PGE1, PGE2 and PGF2alpha on parasympathetic transmission.


Proceedings: Calcium-dependent changes of adenylate cyclase activity by adenosine.


Proceedings: Effects of cyclic nucleotide monophosphates (cAMP, dbcAMP, cGMP, cIMP) on transepithelial netfluxes in proximal convolutions of rat kidneys.


Proceedings: Iso-renin in adrenal glands of rats: a possible factor in steroid production.


Proceedings: The specificity of membranes of chromaffin granules: a biochemical and immunohistological study.


Proceedings: Angiotensin receptors in the adrenal cortex.


Proceedings: The action of tetanus toxin on the electrical activity of the rabbit's brain.


Proceedings: Influence of hydroxyphenyl-orciprenalin (HO) on the cAMP level and the motility of the rat uterus in vitro.


Proceedings: (14C)-labelling and fractionation of lipids in bovine glomerular basement membrane.


Proceedings: Cardiac effects of dibutyryl-C-AMP in several mammalian species.


Proceedings: Investigation of the antilipolytic action of clomiphene.