Presence in leukemic cells of avian myeloblastosis virus-specific DNA sequences absent in normal chicken cells. (3)H-labeled 35S RNA from purified avian myeloblastosis virus (AMV) was exhaustively hybridized with an excess of normal chicken DNA to remove all viral RNA sequences which are complementary to DNA from uninfected cells. The [(3)H]RNA which failed to hybridize was isolated by hydroxylapatite column chromatography which separates DNA-RNA hybrids from single-stranded [(3)H]RNA. The residual RNA hybridized to leukemic chicken DNA but did not rehybridize with normal chicken DNA. This demonstrates conclusively that DNA from AMV-induced leukemic cells contain viral-specific sequences which are absent in DNA from normal cells.


Colitis in mice with a high incidence of rectal prolapse.


Inhibition of herpes simplex virus type 2 replication by thymidine. Replication of herpes simplex virus type 2 (HSV-2) was impeded in KB cells which were blocked in their capacity to synthesize DNA by 2 mM thymidine (TdR). The degree of inhibition was dependent upon the concentration of TdR. In marked contrast, HSV-1 is able to replicate under these conditions. The failure of HSV-2 to replicate is probably due to the inhibition of viral DNA synthesis; there was a marked reduction in the rate of DNA synthesis as well as the total amount of HSV-2 DNA made in the presence of 2 mM TdR. We postulated that the effect of TdR on viral replication occurs at the level of ribonucleotide reductase in a manner similar to KB cells. However, unlike KB cells, an altered ribonucleotide reductase activity, highly resistant to thymidine triphosphate inhibition, was found in extracts of HSV-2-infected KB cells. This activity was present in HSV-2-infected cells incubated in the presence or absence of TdR. Ribonucleotide reductase activity in extracts of HSV-1-infected KB cells showed a similar resistance to thymidine triphosphate inhibition. These results suggest that the effect of TdR on HSV-2 replication occurs at a stage of DNA synthesis other than reduction of cytidine nucleotides to deoxycytidine nucleotides.


Quantitative studies on the migration and development of Taenia pisiformis larvae in laboratory rabbits.


Bacterial diseases of bats: a review.

Bacterial diseases of bats: a review.


Heart rate in caged rhesus monkeys (Macaca mulatta).


A new technical approach for the study of endometrial regeneration in the rabbit.


Comparative aspects of mastocytosis in man and animals with report of a case in a baboon.


The thiolation, methylation, and formation of pseudouridine and dihydrouridine in tRNA of regenerating rat liver, human phytohemagglutinin stimulated lymphocytes, and Novikoff ascites cells.


The changing seborrheic keratosis.

The changing seborrheic keratosis.


Interrelation of epithelial glycogen, cell proliferation and cellular migration with cyclic adenosine monophosphate in epithelial wound healing.


Hyporeactivity of tissue cultures.

Hyporeactivity of tissue cultures.


Radioimmunoassay of insulin by the double antibody method. Evaluation of the techique in practice.


A new indigogenic method for the light and electron microscopic demonstration of 5'-nucleotide phosphodiesterases.


A mitochondrial iron protein with properties of a high-potential iron-sulfur protein.


Histochemical demonstration of xanthine dehydrogenase in the tissues of the domestic fowl.


Unsubstituted agarose as an affinity matrix for isolation of NAD-dependent alpha-glycerophosphate dehydrogenase from the trypanosomatid Crithidia fasciculata.


Mobilization of iron from reticulocyte ghosts by cytoplasmic agents.


Kinetics of Woodward's Reagent K hydrolysis and reaction with staphylococcal nuclease.


Evaluation of mammography based upon correlation of specimen mammograms and histopathologic findings.


Yeast glycogen synthetase in the glucose 6-phosphate-dependent form. I. Purification and properties.


Myosin in cultured fibroblasts.

Myosin in cultured fibroblasts.


The interaction of polypeptide components of human high density lipoprotein with sodium dodecyl sulfate.


Comparison of surface membranes isolated from cultured murine neuroblastoma cells in the differentiated or undifferentiated state.


Childhood cancer: the national Wilm's tumor study. A progress report.


Proceedings: Rhodopsin and light-sensitive kinase activity of retinal outer segments.


Epinephrine and adrenocorticotropic hormone-stimulated magnesium accumulation in adipocytes and their plasma membranes.


Immediate inactivation of T7 coliphage treated by monofunctional alkylating agents.


Rapid diagnostic techniques in clinical virology.


The association of adenovirus infection with disease of the intestinal tract.


Immunoglobulins in virus disease.

Immunoglobulins in virus disease.


Immunofluorescence evidence for the distribution of cyclic AMP in cells and cell masses of the cellular slime molds. With immunofluorescent techniques it has been possible to show that bound cyclic AMP is uniformly distributed in the nucleus and cytoplasm of a number of species of cellular slime molds. One species (which does not respond to cyclic AMP as an acrasin) is an exception and has its cyclic AMP concentrated in the nucleus during the feeding and aggregation stage. In cell masses of Dictyostelium discoideum that show early signs of differentiation the anterior, prestalk cells contain more cyclic AMP than the posterior, prespore cells.


Effects of epinephrine, adrenocorticotrophic hormone, and theophylline on adenosine 3', 5'-monophosphate phosphodiesterase activity in fat cells. When rat fat cells were incubated with ACTH, epinephrine, or theophylline for 2 to 10 min, cyclic AMP phosphodiesterase (3':5'-cyclic-AMP 5'-nucleotidohydrolase, E.C. 3.1.4.17) activity (K(m) about 0.39 muM) in the 100,000 x g sediment fraction of homogenates was increased 35 to 50%. The effects of epinephrine and ACTH were concentration dependent and maximal increases were produced with concentrations similar to those that maximally stimulate lipolysis. Theophylline (0.5 mM) similarly increased phosphodiesterase activity but did not enhance the effects of maximally effective concentrations of the hormones. The changes in phosphodiesterase activity following addition of ACTH or theophylline paralleled changes in cell cyclic AMP content; both reached a maximum within 5 min and then declined, approaching basal levels after 20 or 30 min. The increased phosphodiesterase activity in cells incubated for 5 min with epinephrine reverted to basal levels within 2.5 min after the addition of propranolol. Our data are consistent with the view that there is a component of the fat-cell phosphodiesterase, perhaps localized in the plasma membrane, whose activity can be acutely modified by the concentration of its substrate, cyclic AMP. As previously reported (J. Biol. Chem.248, 7164-7170, 1973), exposure of fat cells to insulin increases the activity of a low K(m) phosphodiesterase also localized in the 100,000 x g sediment fraction of fat cell homogenates. In the presence of insulin, however, phosphodiesterase remained elevated for at least 40 min and there was no significant change in fat-cell cyclic AMP content. When phosphodiesterase activity was elevated and cyclic AMP content maintained at a high level by incubation of cells with ACTH plus theophylline, insulin produced a further increase in enzyme activity. Whether or not insulin and ACTH (or epinephrine or theophylline) affect the same phosphodiesterase, there seems little doubt that the underlying mechanisms are different.


Immune lysis of liposomes and erythrocyte ghosts loaded with spin label. It is shown that the paramagnetic resonance of spin labels offers a convenient technique for monitoring the complement-mediated immune lysis of erythrocyte ghosts, and sensitized phospholipid liposomes. The ghosts or liposomes are loaded with a concentrated solution of a water-soluble, membrane-impermeable spin label, so that there is a strong exchange broadening of the paramagnetic resonance signal due to labels enclosed within the liposomes or ghosts. Complement-mediated lysis releases the labels into a dilute solution, where a relatively sharp and intense paramagnetic resonance signal is detected. AN UNANTICIPATED RESULT IS ALSO REPORTED: this is the augmentation of release of spin label from loaded ghosts sensitized with egg albumin, in the presence of antibody against egg albumin, complement, and egg albumin in solution, over and above the release in the absence of egg albumin in solution. Comparison with complement fixation suggests that the augmentation of lysis is effected by crosslinking of immune complexes with the cell membrane.


Angiotensin-converting enzyme from rabbit pulmonary particles. Angiotensin-converting enzyme has been solubilized from rabbit pulmonary particles and purified to homogeneity. The molecular weight of the native enzyme was estimated to be about 136,000 by glycerol gradient centrifugation, and a value of 140,000 was obtained for the reduced denatured protein by disc-gel electrophoresis in the presence of sodium dodecyl sulfate and 2-mercaptoethanol. The enzyme was found to be a glycoprotein with carbohydrate accounting for approximately 16% of its dry weight. The major sugar residues were identified as galactose, N-acetylglucosamine, and mannose, with smaller amounts of fucose and sialic acid. The homogeneous enzyme catalyzed the release of His-Leu from the COOH-terminus of angiotensin I and of Phe-Arg and Ser-Pro from that of bradykinin.


Interspecific recombination of mitochondrial DNA molecules in hybrid somatic cells. The mitochondrial DNA (mtDNA) in rodent-human hybrid somatic cells was studied in strains that contain nucleotide sequences from both parental mtDNAs. A test for linkage of rodent to human mtDNA was devised on the basis of the density and sequence differences between these DNAs. When a mixture of rodent and human mtDNAs was banded in a CsCl gradient and each fraction hybridized with a mixture of complementary [(3)H]RNA transcribed from human mtDNA and complementary [(32)P]RNA transcribed from rodent mtDNA, each DNA was detected as a distinct and separate band at its expected density by specific hybridization with its complementary RNA. In some of the hybrid cell strains, the mtDNA sequences derived from the two species did not separate in the CsCl gradients. This result is interpreted as evidence for linkage between sequences from the two parental mtDNAs. While the exact nature of the linkage and the structure of the molecules containing both types of sequences are not known, the evidence supports the conclusion that the linkage is realized by a covalent bond. An event leading to the covalent bonding of these different sequences may be described as recombination. Among 18 hybrid cell strains examined, 13 contained large proportions of recombinant molecules. These molecules were found in both mouse-human and rat-human strains, and in strains containing more human or more rodent mtDNA sequences.


Characterization and substrate specificity of a protein carboxymethylase in the pituitary gland. Protein carboxymethylase, an enzyme capable of methylating proteins and polypeptides, was purified from bovine pituitary. The anterior pituitary hormones, luteinizing hormone, follicle-stimulating hormone, adrenocorticotropic hormone, growth hormone, thyroid-stimulating hormone, and prolactin, were found to be substrates for this enzyme. The posterior pituitary hormones, oxytocin and vasopressin, did not serve as substrates. With luteinizing hormone as the substrate, protein carboxymethylase had a pH optimum near pH 5.5. A limiting K(m) of 1.47 muM for S-adenosyl-L-methionine was obtained with luteinizing hormone as the methyl acceptor. Possible roles of this enzyme in the posterior and anterior pituitary are discussed.


Cyclic-AMP-controlled oscillations in suspended Dictyostelium cells: their relation to morphogenetic cell interactions. Periodic spikes of decreased optical density were recorded in cell suspensions of Dictyostelium discoideum. Spike formation as well as changes in the redox state of cytochrome b are facultatively and independently coupled to an oscillating system which is under developmental control and presumably underlies signal transmission in aggregating cells. Cyclic AMP triggers a double response, the slow component resembling the spikes formed during spontaneous oscillations. The fast component shows characteristics of the chemotactic response to cyclic AMP. The receptor system is suggested to sense changes of cyclic AMP concentration in time. Cyclic AMP pulses interact with the oscillating system, resulting in phase shift or suppression of spike formation, and in the induction of oscillations in an early stage of development before the onset of spontaneous oscillations. Continuous flow application of cyclic AMP does not change frequency up to flow rates which extinguish oscillations.


Estrogen-related increases in uterine guanosine 3':5'-cyclic momophosphate levels. Endogenous uterine cyclic GMP and cyclic AMP concentrations were monitored at each stage of the estrus cycle; cyclic GMP concentrations were found to be highest and cyclic AMP concentrations lowest during proestrus when plasma estrogen levels have been shown to be maximal. After administration of estradiol-17beta or diethylstilbestrol, the concentrations of uterine cyclic GMP in ovariectomized rats underwent a progressive and significant elevation and the levels of cyclic AMP declined significantly below control values when near maximal accumulation of tissue cyclic GMP was achieved. The increase in uterine cyclic GMP concentrations produced by estradiol benzoate administration was prevented when progesterone and the estrogen were co-administered. These results raise the possibility that an enhanced cellular accumulation of cyclic GMP may be involved in the expression of some of the actions of estrogen in uterine tissue.


Simultaneous administration of dichlorvos anthelmintic and modified live virus vaccines to puppies.


A study of serum vitamin B12 in various peripheral neuropathies.


Third F. Raymond Keating, Jr., Memorial Symposium--parathyroid hormone, calcitonin and vitamin D: clinical considerations. II. Vitamin D--1973.


Rickets and the pathogenesis of impaired tubular transport of phosphate and other solutes.


The physiologic significance of plasma transport of vitamin D and metabolites.


Circulating 25-hydroxyvitamin D in man.

Circulating 25-hydroxyvitamin D in man.


Intrauterine infection and cord immunoglobulin M. 3. Serological analysis of infants with elevated cord serum immunoglobulin M. The presence of antibodies to rubella, cytomegalovirus and Toxoplasma gondii was determined at birth and at 6 months of age in a group of 147 infants with cord serum IgM levels >/= 19.0 mg/dl and in 92 control infants. Maternal syphilis serology was determined in both groups as well. No significant differences in the prevalence or levels of antibodies to these pathogens were found between the two groups which might have led to the diagnosis of unsuspected intrauterine infection. Persistence of antibodies to 6 months of age was similar in the two groups, indicating that this is not a useful index of intrauterine infection.ANALYSIS OF THE RESULTS YIELDED THE FOLLOWING DATA ON THE PREVALENCE OF ANTIBODIES TO THE PATHOGENS STUDIED: rubella virus, 90 and 75% seropositivity at birth and 6 months respectively; cytomegalovirus, 65 and 35%; and Toxoplasma gondii, 33% seropositivity at birth.


Transport of peroxidase in flounder kidney tubules studied by electron microscope histochemistry.


Sedative or tranquillizer? A comparison of the hypnotic effects of chlordiazepoxide and amylobarbitone sodium.


Nuclear gene dosage effects on mitochondrial mass and DNA. In order to assess the effect of nuclear gene dosage on the regulation of mitochondria we have studied serial sections of a set of isogenic haploid and diploid cells of Saccharomyces cerevisiae, growing exponentially in the absence of catabolite repression, and determined the amount of mitochondrial DNA per cell. Mitochondria accounted for 14% of the cytoplasmic and 12% of the total cellular volume in all cells examined regardless of their ploidy or their apparent stage in the cell cycle. The mean number of mitochondria per cell was 22 in the diploid and 10 in the haploids. The volume distribution appeared unimodal and identical in haploids and diploids. The mitochondrial DNA accounted for 12.6 +/- 1.2% and 13.5 +/- 1.3% of the total cellular DNA in the diploid and haploid populations, respectively. These values correspond to 3.6 x 10(-15) g, 2.2 x 10(9) daltons, or 44 genomes (50 x 10(6) daltons each) per haploid and twice that per diploid cell. On this basis, the average mitochondrion in these cells contains four mitochondrial genomes in both the haploid and the diploid.


Lysosomal packaging in differentiating and degenerating anuran lateral motor column neurons. The role of the Golgi apparatus and the Golgi-endoplasmic reticulum-lysosome complex (GERL) in the genesis of lysosomes was examined in differentiating and degenerating motor neurons of anuran larvae. Acid phosphatase, aryl sulfatase, and thiolacetic acid esterase were utilized as marker enzymes for the lysosomal system, while nucleoside diphosphatase and thiamine pyrophosphatase labeled the inner saccule(s) of the Golgi apparatus. Reduced osmium tetroxide was routinely deposited in the outer Golgi saccule regardless of the state of neuronal maturation. In all young neurons, the disposition of acid hydrolase reaction product paralleled the formation of GERL, with no lytic activity in the Golgi apparatus per se. Hypertrophy of the Golgi apparatus and GERL was observed in the early phases of degeneration, and both organelles apparently exhibit extensive hydrolytic activity. Dense bodies, autophagic vacuoles, and primary lysosomes were found arising from GERL, while the Golgi apparatus may produce primary lysosomal granules during regression. On the other hand, in differentiating neurons, hydrolytic activity was restricted to GERL and an occasional dense body and autophagic vacuole. These studies illustrate a parallelism between the development of GERL and genesis of primary and secondary lysosomes during neuronal cytodifferentiation, and implicate GERL and possibly the Golgi apparatus in lysosomal packaging in degenerating neurons.


Quinoline derivatives as antiallergy agents.


Thymidine 5' variants as inhibitors of thymidylate kinase.


Morphology of mesenchymal elements of normal artery, fatty streaks, and plaques.


Studies of primate arterial smooth muscle cells in relation to atherosclerosis.


Changes in plasma levels of estrone sulfate and estrone in the pregnant ewe around parturition.


Fatal herpes hepatitis associated with pemphigus vulgaris and steroids in an adult.


Dugbe virus: a tick-borne arbovirus from Nigeria.


Thiamine diphosphate content of liver mitochondria of the pregnant and developing rat.


Partial separation of Sendai virus-fused lymphocytes by velocity sedimentation.


Intracellular concentration of inorganic pyrophosphate in the cells of Escherichia coli: a method for its determination.


Riboflavin deficiency and galactose metabolism in human subjects.


A microassay for guanosine 3',5'-monophosphate phosphodiesterase activity.


Microsomal Na+-stimulated NADH-cytochrome c reductase: could it be involved in sodium transport?


Assay of cyclic AMP by an isotope dilution test with binding protein.


Coprecipitation of ATP with potassium perchlorate: the effect of the firefly enzyme assay of ATP in tissue and blood.


Separation and automated analysis of phosphorylated metabolic intermediates.


Langerhans cells in a bronchiolar-alveolar tumour of lung.


A statistical analysis of the release of acetylcholine at newly formed synapses in striated muscle. 1. A statistical analysis has been made of the changes in the binomial parameters n and p during transmitter release from motor-nerve terminals when synapses are forming in either regenerating or reinnervated mammalian muscle.2. The amplitude-frequency distribution of e.p.p.s evoked by a single impulse was well predicted by binomial statistics at all junctions analysed allowing values of n and p to be determined. At most of these junctions, p > 0.5 and Poisson statistics did not predict the observed distributions at all.3. During the changes in quantal content which occur during short trains of impulses or during continual stimulation over minutes, no significant change in p was detected. Changes in quantal content of the e.p.p. were therefore determined by n.


Sulfhydryl group reactivity of D-galactose dehydrogenases from Pseudomonas saccharophila.


28-S RNA from Xenopus laevis contains a sequence of three adjacent 2'-O-methylations.


Aspiration biopsy cytology. I. Cytology of supradiaphragmatic organs. 8 Lungs.


Characterization of blood-group-H-active ceramide tetrasaccharide from hog-stomach mucosa.


Proceedings: Plasma levels of amino acids and glucagon in patients with pancreatic glucagonomas.


Editorial: Vitamin D, the kidney and calcium homeostasis.


Fine-structural observations on the gill filaments of the fresh-water crayfish, Astacus pallipes Lereboullet.


The effect of exogenous angiotensin on superficial and deep glomeruli in the rat kidney.


An evaluation of the contribution of cholinergic mechanism to thirst.


Centrally mediated effects of angiotensin II in the unanesthetized rat.


Studies on Coccidioides immitis. XVII: Morphology of the parasite in relationship with the host immunoallergic condition in the experimental infection of the guinea-pig.


Occurrence of atypical lymphocytes following intravenous injection of Candida albicans in mice.


Comparative treatment of hyperlipoproteinaemias in chimpanzees.


Glycosylation of beta-lipoprotein and the possible role of choline derivatives.


Enzymatic dehydrogenation of testosterone coupled to pyruvate reduction in a two-phase system.


Urinary excretion of cyclic AMP by cold-acclimated rats.


Nucleophilic addition at the photoexcited flavin cation: synthesis and properties of 6- and 9-hydroxy-flavocoenzyme chromophores.


Identification and properties of new flavins in electron-transferring flavoprotein from Peptostreptococcus elsdenii and pig-liver glycolate oxidase.


Regulation of myocardial contraction.

Regulation of myocardial contraction.


Free fatty acids and the ischaemic myocardium.


Lymphocytes in infectious mononucleosis. Properties of atypical cells and origin on the lymphoblastoid lines.


Regulatory role of adenyl cyclase and cyclic AMP.


Urea-cycle enzymes in normal liver and in patients with alcoholic hepatitis.


Effect of onion ingestion on serum triglyceride, betalipoprotein-cholesterol and phospholipids in alimentary lipaemia.


Coeliac disease. Electron microscopy of jejunal mucosa.


Cytomegalovirus infection after lumbosacral fusion. A case report.


Editorial: Sex and alcohol.

Editorial: Sex and alcohol.


Influence of prostaglandin E1 on cyclic 3',5'-AMP levels and glycosaminoglycan secretion of fibroblasts cultured in vitro.


Studies on human cytomegalovirus strain variations by membrane-fluorescence.


Astrocytosis without globules in infant cerebral white matter. An epidemiologic study.


Vitreoretinal juncture--simple epiretinal membranes.


Effect of dipyridamole on retinal embolism associated with cardiopulmonary bypass surgery in the dog.


Serum prolactin levels during ovulatory menstrual cycle and menstrual disorders in women.


Rubella and cytomegalovirus. Current concepts of congenital and acquired infection.


Influence of antiphlogistic drugs on prostaglandin E1 stimulated cyclic 3',5'-AMP levels and glycosaminoglycan synthesis in fibroblast tissue cultures.


Adynamic ileus, its pathogenesis and treatment.


Plasma renin and angiotensin I converting enzyme activity in anaphylactic shock.


Effects of prostaglandin synthesis inhibitors on angiotensin tachyphylaxis in the isolated coeliac and mesenteric arteries of the rabbit.


A human papovavirus (B.K.), biological properties and seroepidemiology.


Abnormal reflexes in the "stiff-man" syndrome.


A roentgenologic study of epiphyseal union around elbow, wrist and knee joints and the pelvis in boys and girls of Uttar Pradesh.


Guanidine inhibition of poliovirus growth. Partial elimination by protease antagonists and low temperature.


Studies on beta-galactosidase activity in Vibrio El Tor.


Biochemical studies on sulfate-ruducing bacteria. 8. Sulfite reductase from Desulfovibrio vulgaris--mechanism of trithionate, thiosulfate, and sulfide formation and enzymatic properties.


The response of adipose tissue to lipid mobilizing hormones in postnatally under- and overnourished rats.


The activity of adenylate cyclase, phosphodiesterase and protein kinase in the adenohypophyses of rats treated by chronic administration of estrogens and acute application of thyroxine in vivo.


Two types of insulin receptors mediating opposite effects on cyclic-amp in fat cells of the rat.


Effect of insulin on the phosphorylation of adipose tissue protein.


Gastric HCO3-stimulated ATPase: evidence against its microsomal localization in rat fundus mucosa.


Testosterone-estradiol-binding globulin of human plasma: denaturation and protection.


Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.


Synthetic analogs of the active sites of iron-sulfur proteins. 8. Some electronic properties of (Fe4S4(SR)4)3-, analogs of reduced bacterial ferredoxins.


A possible mechanism for the stimulation of Krebs cycle activity by ACTH in isolated rat adrenal cells.


The influence of 2,3 diphosphoglycerate on the Bohr effect of sulfhemoglobin.


Peptidase activity of carrier proteins used in radioimmunoassays.


Pathomorphologic and histochemical changes in the liver during hemodilution.


Regulation of glycogen metabolism in acute uremic hearts.


Catecholamine metabolism in health and disease.


Biochemical genetics of bacterial sporulation. V. Fine structure analysis of two sporulation mutants of B. subtilis derepressed for late functions.


Saponin adjuvants. 3. Isolation of a substance from Quillaja saponaria Molina with adjuvant activity in food-and-mouth disease vaccines.


The development of double-seeded and mixed cell culture systems for use in diagnostic virology.


Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture.


Effect of morphinization on in vitro incorporation of labelled precursors into adrenal protein and nucleic acids in rats.


Adrenergic mechanism in Tetrahymena. 3. Cyclic adenosine 3', 5'-monophosphate and cell proliferation.


Differentiation between early and late membrane antigen on human lymphoblastoid cell lines infected with Epstein-Barr virus. I. Immunofluorescence.


Latent infection and malignant lymphoma in marmosets (Callithrix jacchus) after infection with two oncogenic herpesviruses from primates.


Pancreatic islet cell tumors in domestic animals. Data from 11 colleges of veterinary medicine in the United States and Canada.


Presence and transmission of mammary tumor virus and leukemia virus in the BALB-cfRIII mouse.


Variation in quantitative expression of Forssman antigen on virus-transformed hamster cells.


Elevated cyclic AMP levels in human breast-cancer tissue.


Zinc intake and growth of a transplanted hepatoma induced by 3'-methyl-4-dimethylaminoazobenzene in rats.


Acute cerebral infarction and hypotension: an ultrastructural study.


The astrocyte response in experimental portal-systemic encephalopathy: an electron microscopic study.


Electron-microscopic observations of scirrhous and mucin-producing carcinomas of the breast.


Gliomas containing bone and cartilage.

Gliomas containing bone and cartilage.


The nature of the circadian periodicity and suppressibility of immunoreactive ACTH levels in Addison's disease.


Cyclic adenosine 3',5'-monophosphate concentration in plasma, adipose tissue and skeletal muscle in normal subjects and in patients with hyper- and hypothyroidism.


Secretion of 16alpha-hydroxyprogesterone and other steroids by the adult human adrenal.


Regulation of aldosterone in the syndrome of inappropriate antidiuretic hormone secretion (SIADH).


Human adrenal tissue in vitro: steroidogenic properties.


Cirrhosis and malignant hepatoma in alpha 1-antitrypsin deficiency.


Respirable silica dust exposure of sandblasters and associated workers in steel fabrication yards.


Effects of cigarette smoke components on bronchial clearance in the donkey.


Wilm's tumour.

Wilm's tumour.


Intranuclear inclusions in the lead-poisoned cultured kidney cell.


Cytophysiology and metabolism of pancreatic B-cell.


Plasma MSH and cortisol levels in 567 patients with special reference to brain trauma.


Immediate effects of total visual deafferentation on single unit activity in the visual cortex of freely behaving cats. II. Rhythmic EEG bursts and PGO waves.


Immediate effects of total visual deafferentation on single unit activity in the visual cortex of freely behaving cats. I. Tonic excitability changes.


Clostridium perfringens panophthalmitis.


Tumours of the orbit and the eye in Indonesia. A clincal-pathological correlation.


Methyl n-butyl ketone.

Methyl n-butyl ketone.


Treatment of advanced primary liver carcinoma using intermittent intra-arterial nitrogen mustard.


Genital herpes in guinea-pigs. An experimental model with Herpesvirus hominis.


Simian virus 40 in a human cancer.

Simian virus 40 in a human cancer.


Metabolism and action of the hormone vitamin D. Its relation to diseases of calcium homeostasis. Extensive experimental evidence has established a significant role of calciferol in the maintenance of normal calcium homeostasis. Present knowledge indicates that vitamin D(3) must first be converted to 25-OH-D(3) and then to 1,25(OH)(2)D(3), the most active known form of the steroid. Many of the factors regulating the rate of production of this last steroid from its precurser have been evaluated, and the concept that vitamin D functions as a steroid hormone seems to be well established. Deranged action of calciferol, caused by impaired metabolism of the steroid or through altered sensitivity of target tissues, may be involved in the pathophysiology of several disease states with abnormal calcium metabolism. It is noted that liver disease, osteomalacia due to anticonvulsant therapy, chronic renal failure, hypophosphatemic rickets, hypoparathyroidism, hyperparathyroidism, sarcoidosis and idiopathic hypercalciuria have possible relation to alterations in metabolism or action of vitamin D. The future clinical availability of 1,25(OH)(2)D(3) and other analogs of this steroid may offer potential therapeutic benefit in the treatment of certain of the disease entities discussed.


Outline structure of cytochrome c3 and consideration of its properties.


Congenital corneal clouding with abnormal systemic storage bodies: a new variant of mucolipidosis.


Serologic responses to herpes simplex virus in rabbits: complement-requiring neutralizing, conventional neutralizing, and passive hemagglutinating antibodies.


Pathogenesis of pulmonary cytomegalovirus infection in immunosuppressed mice.


The effect of cholera enterotoxin on steroidogenesis in cultured adrenal tumor cells.


Bacterial interference among strains of Staphylococcus aureus in man.


Urinary tract infection and pyelonephritis due to Escherichia coli in dogs infected with canine adenovirus.


Fecal excretion of cytomegalovirus in disseminated cytomegalic inclusion disease.


Plasma proinsulin. Comparison of insulin specific protease and gel filtration assays.


Influence of dietary protein on 6- and 9-desaturation of fatty acids in rats of different ages and in different seasons.


Occurrence of Parkinson's disease in black patients at Charity Hospital in New Orleans.


Lipid cerebrospinal fluid in a patient with cerebral hemorrhage.


Plasma vitamin A and E levels in children with familial type II hyperlipoproteinemia during therapy with diet and cholestyramine resin.


Sleep epilepsy: its pattern and prognosis. In a series of 645 patients with epilepsy only 38 had attacks exclusively during sleep. A further 111 had attacks while waking and sleeping. The total number of patients whose epilepsy started with sleep attacks and who later developed attacks when waking increased with each successive year. The prognosis of sleep epilepsy is important in relation to the granting of driving licences.


Letter: Unsuspected cytomegalic mononucleosis.


Vitamin A and glycosaminoglycan metabolism in rats.


Relation of hypertension to changes in the arteries.


EB virus and the treatment of lymphomas. The high incidence of EB virus infections (and possibly of other viruses) in patients with African lymphoma and, to a lesser extent, in patients with lymphosarcoma in the temperate zones, is thought to be due to its being a passenger virus which persists in the human body and, since it is lymphotropic, presents an opportunistic infection of proliferating lymphoid cells. The thesis is put forward that by entering the cell nucleus, the virus renders malignant lymphoid cells more vulnerable to cytotoxic drugs. Hence a deliberate policy of EB virus inoculation before starting chemotherapy might achieve a more rapid response and longer remissions in indigenous lymphomas of the temperate zones, and might possibly render drug-resistant cases once more drug-sensitive.


Renin and aldosterone and the pathogenesis of hypertensive vascular damage.


Letter: Mental changes in Parkinsonism.

Letter: Mental changes in Parkinsonism.


An unusual case of adenocarcinoma of the pancreas.


Metronidazole and amebiasis.

Metronidazole and amebiasis.


A histological, histochemical and biochemical study of the effect of the toxin of lupinosis on murine liver.


Plasma corticotrophin levels during insulin-hypoglycaemia: comparison of radioimmunoassay and cytochemical bioassay.


Mass determination of lipids with automated thermogravimetric analytical procedure.


DDT absorption and chylomicron transport in rat.


Radioimmunoassay for medroxyprogesterone acetate (Provera) using the 11alpha-hydroxy succinyl conjugate. The development of a radioimmunoassay for medroxyprogesterone acetate (MPA) employing rabbit antibodies made against a bovine serum albumin (BSA) conjugate of the hemisuccinate ester of 11alpha-hydroxy MPA are described.  The 11alpa-hydroxyl group was introduced into MPA by a series of chemical and microbiological transformations.  The acid succinate MPA was coupled to BSA by reacting it with N,N'-carbonyldiimidazole.  Cross-reactivities of several MPA analogs were compared suing rabbit antisera developed against the C-11 conjugate of MPA and goat antiserum developed against a C-3 conjugate of MPA.  Antibodies formed against the C-11 conjugate showed increased specificity to steroid analogs which changes in the C-21 side chain.  The profiles of serum MPA levels vs time, measured with both the C-3 and C-11 antisera, after oral drug administration to the monkey were similar.  After intramuscular drug administration to dogs, serum MPA levels measured with the C-3 antisera were consistently greater as compared with those with C-11 antisera.  Factors affecting precipitation of the antibody-antigen complex, assay precision, and assay sensitivity were evaluated.


The cytochemical section assay for corticotrophin.


Ectopic hormone production by non-endocrine tumours.


The redox bioassay technique for the direct assessment of pituitary adrenocorticotrophic activity in the rat.


Validation of the redox cytochemical assay for corticotrophin.


Massive fetal ascites, urethral atresia, and cytomegalic inclusion disease.


Virus-like particles induced in guinea pig cells by 5-bromo-2'-deoxyuridine are morphologically similar to murine B-type virus.


Evidence that most noradrenaline is stored without ATP in sympathetic large dense core nerve vesicles.


Individual versus group aftercare treatment.


Mixed Müllerian tumors of the oviduct.

Mixed Müllerian tumors of the oviduct.


Auxiliary structures of tuberous organ (electroreceptor) of Sternarchus albifrons (Gymnotidae).


The giant melanosome: a model of deranged melanosome-morphogenesis.


Striated and crystalline inclusions in the nuclei and cytoplasm of intact yeast cells and yeast protoplasts.


Letter: Acute poliomyelitis disputed.

Letter: Acute poliomyelitis disputed.


Cerebral blood flow in acute hypertension.


Proceedings: Interactions of separate types of cells during normal and neoplastic mammary gland growth.


Proceedings: Hormones and other factors controlling growth in the mammary gland: a review.


Special diet for familial type II hyperlipoproteinemia.


Proceedings: Mammogenesis in transsexuals.


Bone marrow biopsy in malignant disease.


Pitfalls in the diagnosis of low-back and leg pain.


Brain scanning and conventional neuroradiologic methods in intracranial tumours. Scanning with 99Tcm and a gamma camera.


Experience with intraoperative echoencephalography in cerebral mass lesions.


Cerebral scintiphotos with 113mIn.

Cerebral scintiphotos with 113mIn.


Unusual myelographic appearances of the lumbosacral radicular sheaths.


Electron microscopic observations of human retinitis pigmentosa, dominantly inherited.


Retinal degeneration in monkeys induced by deficiencies of vitamin E or A.


Primary malignant lymphoma of the bladder: case report and review of the literature.


Nuclear bodies in papillary carcinomas of the human thyroid gland.


Association between herpes hominis type 2 and the male genitourinary tract.


Megakaryocytes in the May-Heggelin anomaly.


Hepatic injury associated with penicillin therapy.


Functional black adenoma of the adrenal gland. Light and electron microscopical study.


An electrophoretic study of the negative correlation of certain carboxylesterases to insecticide resistance in Musca domestica L.


Electron microscope studies on the dissociation of actomyosin by pyrophosphate.


Effects of GABA on presynaptic nerve terminals in bullfrog (Rana catesbiana) sympathetic ganglia.


Light-microscopic demonstration of drug-induced myelin bodies in the liver of rats.


A renal tumour of the nephroblastoma type in Praomys (Mastomys) natalensis.


Excretion of equine infectious anemia virus from horses infected with the virus.


In vitro synthesis of an ACTH-like hormone and human chorionic somatomammotrophin by placental and amniotic cells.


Studies on the replicase of encephalomyocarditis virus.


SV40 DNA molecules which contain host cell DNA.


Prostaglandins and cancer: an update.

Prostaglandins and cancer: an update.


Cyclic AMP, prostaglandins, and the control of cell proliferation.


Effects of prostaglandins on the stomach and the intestine.


Morphology of the electromotor system in the spinal cord of the electric eel, Electrophorus electricus.


Synaptic organization of the substantia gelatinosa glomeruli in the spinal trigeminal nucleus of the adult cat.


Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40. Temperature-sensitive mutants of simian virus 40 (SV40) have been classified as those that are blocked prior to viral DNA synthesis at the restrictive temperature, "early" mutants, and those harboring a defect later in the replication cycle, "late" mutants. Mutants of the A and D complementation groups are early, those of the B, C, and BC groups are late. Our results confirm earlier reports that A mutants are defective in a function required for the initiation of each round of viral DNA synthesis. D mutants, on the other hand, continue viral DNA replication at the restrictive temperature after preincubation at the permissive temperature. The length of time required for D function to be expressed at the permissive temperature-after which infection proceeds unabated on shifting of the cultures to the restrictive temperature-is 10 to 20 h. The viral DNA synthesized in D mutants under these conditions progresses in normal fashion through replicative intermediate molecules to mature component I and II DNA molecules.


Genetic analysis of a temperature-resistant revertant of the conditional lethal Escherichia coli double mutant polA12 uvrE502. Conditional lethality of the Escherichia coli polA12 uvrE502 double mutant may be overcome by a mutation that has been termed polA350. The polA350 mutation restored the polymerizing activity of deoxyribonucleic acid polymerase I at 42 C in the polA12 mutant and partially suppressed ultraviolet (UV) and methylmethane sulfonate sensitivities of the polA12. Mapping experiments have located polA350 between metE and polA12, very close to the latter. The strain carrying polA12 polA350 and recB21 was viable at 42 C. The effects of the recB21 and polA12 polA350 combination on the UV sensitivity were additive. The triple mutant polA12 polA350 uvrE502 was more UV sensitive than the single uvrE502 mutant.


Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus: partial characterization of the enzyme. Some of the catalytic properties of the biosynthetic dihydroorotate dehydrogenase purified from an anaerobic bacterium, Lactobacillus bulgaricus, are described. Studies with p-hydroxymercuribenzoate, N-ethylmaleimide, and mercuric chloride showed that sulfhydryl groups are necessary for transfer of electrons from dihydroorotate to a variety of electron acceptors. Protection studies with substrates for the enzyme indicated that free sulfhydryl groups at or near the active center are required for catalytic activity. Evidence is presented for the production of superoxide free radicals during reaction of the enzyme with molecular oxygen. Inhibitor studies with Tiron indicated that reduction of cytochrome c by the enzyme may involve the superoxide free radical as an intermediate. Orotate, one of the substrates for the enzyme, has been found to be a competitive inhibitor for the dihydroorotate site. The K(i) for orotate as estimated by several techniques is 0.1 mM. The K(m) for dihydroorotate with ferricyanide as the electron acceptor is estimated to be 0.5 mM.


Pleiotropic alteration of activities of several toxins and enzymes in mutants of Staphylococcus aureus. Pleiotropic alteration of several genetic characters including toxin production was quantitatively shown with a strain of Staphylococcus aureus of phage type 80, 81 which had been given a very specific genetic marker (temperature sensitivity of mannitol fermentation) to avoid confusion by contamination. Thus, alpha-hemolysin hyperproducers obtained by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis were very often hyperproducers of DNase, coagulase, and protease. Their colonies were less yellow than the parent. DNase hyperproducers obtained after NTG mutagenesis were also often hyperproducers of alpha-hemolysin, coagulase, and protease, with colonies less yellow than the parent. Almost all of the revertants obtained by mutagenesis with ethyl methane sulfonate with respect to alpha-hemolysin or DNase were shown to have simultaneously become hypoproducers of alpha-hemolysin, DNase, and protease. Since the pleiotropic alteration of multiple functions was thus quantitatively confirmed, the mechanism underlying this phenomenon should probably be related to a regulatory mechanism common to them.


Ribitol catabolic pathway in Klebsiella aerogenes. In Klebsiella aerogenes W70, there is an inducible pathway for the catabolism of ribitol consisting of at least two enzymes, ribitol dehydrogenase (RDH) and d-ribulokinase (DRK). These two enzymes are coordinately controlled and induced in response to d-ribulose, an intermediate of the pathway. Whereas wild-type K. aerogenes W70 are unable to utilize xylitol as a carbon and energy source, mutants constitutive for the ribitol pathway are able to utilize RDH to oxidize the unusual pentitol, xylitol, to d-xylulose. These mutants are able to grow on xylitol, presumably by utilization of the d-xylulose produced. Mutants constitutive for l-fucose isomerase can utilize the isomerase to convert d-arabinose to d-ribulose. In the presence of d-ribulose, RDH and DRK are induced, and such mutants are thus able to phosphorylate the d-ribulose by using the DRK of the ribitol pathway. Derivatives of an l-fucose isomerase-constitutive mutant were plated on d-arabinose, ribitol, and xylitol to select and identify mutations in the ribitol pathway. Using the transducing phage PW52, we were able to demonstrate genetic linkage of the loci involved. Three-point crosses, using constitutive mutants as donors and RDH(-), DRK(-) double mutants as recipients and selecting for DRK(+) transductants on d-arabinose, resulted in DRK(+)RDH(+)-constitutive, DRK(+)RDH(+)-inducible, and DRK(+)RDH(-)-inducible transductants but no detectable DRK(+)RDH(-) constitutive transductants, data consistent with the order rbtC-rbtD-rbtK, where rbtC is a control site and rbtD and rbtK correspond to the sites for the sites for the enzymes RDH and DRK, respectively.


Isolating peripheral nerve activity with respect to propagation velocity.


Regulation of rabbit liver fructose-1,6-diphosphatase. II. Modification by lysosomal cathepsin B1 from the same cell.


D-Arabitol catabolic pathway in Klebsiella aerogenes. Klebsiella aerogenes strain W70 has an inducible pathway for the degradation of d-arabitol which is comparable to the one found in Aerobacter aerogenes strain PRL-R3. The pathway is also similar to the pathway of ribitol catabolism in that it is composed of a pentitol dehydrogenase, d-arabitol dehydrogenase (ADH), and a pentulokinase, d-xylulokinase (DXK). These two enzymes are coordinately controlled and induced in response to d-arabitol, the apparent inducer of synthesis of these enzymes. We obtained mutants which lacked a functional d-xylose pathway and were constitutive for the ribitol catabolic pathway. These mutants were able to grow on the unusual pentitol, xylitol, only if they contained the functional DXK of the d-arabitol pathway. This provided us with a specific selection technique for DXK(+) transductants. As in A. aerogenes, mutants constitutive for ADH were able to use this enzyme to convert the hexitol d-mannitol to d-fructose. With mutants blocked in the normal d-mannitol catabolic pathway, growth on d-mannitol became a test for ADH constitutivity. Growth of such mutants on xylitol, d-arabitol, and d-mannitol was utilized to classify transductants in mapping, by transductional analysis, the loci involved in d-arabitol utilization. Three-point crosses gave the order dalK-dalD-dalC, where dalK is the DXK structural gene, dalD is the ADH structural gene, and dalC is a regulatory site controlling synthesis of both enzymes.


Morphologic abnormalities in human infant cerebral white matter related to gestational and postnatal age.


Ultrastructural changes in germinating ascospores of Saccharomyces cerevisiae.


The cytochrome system in Mycobacterium lepraemurium.


Histoenzymological demonstration of certain phosphatases in the intestinal mucosa of common garden lizard Calotes versicolor.


Hypoglycemia with primary liver cell carcinoma.


The acupunture point.

The acupunture point.


The influence of presleep suggestions on dream content: evidence and methodological problems.


Proceedings: The oxidation of catecholamines by hemepeptide obtained from enzymatically digested yeast cytochrome C.


Hepatic cell adenoma: A report of four cases. Four patients with hepatic cell adenoma have been treated at the UCLA Hospital since 1965. The most recent was a 22-year-old woman who underwent subtotal resection of a giant hepatic cell adenoma after an unusual and confusing clinical presentation. The tumor may be the largest reported to date and may have excreted metabolically-active substances. Increased familiarity with the varying clinical and radiographic presentations of these rare tumors may facilitate earlier diagnosis and management.


Ultrastructure of the fetal rat adenohypophysis.


The retina and optic nerve.

The retina and optic nerve.


The effect of plasma cortisol concentration on the pituitary adrenal response elicited by electrical stimulation of the infundibular area of the awake unrestrained cat.


Vasodilator drugs in the treatment of peripheral arterial insufficiency.


Experimental studies of the endolymphatic radiotherapy.


Expression of the familial hypercholesterolemia gene in heterozygotes: mechanism for a dominant disorder in man. Studies in ctltured fibroblasts indicate that the primary genetic abnormality in familial hypercholesterolemia involves a deficiency in a cell surface receptor for low density lipoproteins (LDL). In normal cells, binding of LDL to this receptor regulates cholesterol metabolism by suppressing cholesterol synthesis and increasing LDL degradation. In cells from heterozygotes, a 60 percent reduction in LDL receptors leads to a concentration-dependent defect in regulation, so that attainment of equal rates of cholesterol synthesis and LDL degradation in normal and heterozygous cells requires a two- to threefold higher concentration of LDL in the heterozygote. The identification of this genetic regulatory defect in fibroblasts of heterozygotes makes available an in vitro system for studying the effects of a dominant mutation on gene expression in mammalian cells.


The effects of Escherichia coli endotoxin and ACTH on the plasma zinc concentration in the domestic fowl.


Collagenolytic enzymes and acid-proteinase activity in granuloma tissue.


Loss of serum protein from hepatoma-bearing animals.


The lipoprotein apoproteins. Their role in normal lipid transport and dyslipoproteinemia.


Comparison of MHG virus with mouse encephalomyelitis viruses.


Neuromuscular transmission in myasthenia gravis studied with single fibre electromyography. Patients with myasthenia gravis have been investigated with single fibre electromyography. In all myasthenic muscles recordings were obtained where there was an increase of the jitter and occasional blocking of different degree of single fibre action potentials. The jitter is the variability at consecutive discharges in the time interval between action potentials from two muscle fibres from the same motor unit. During prolonged activity the jitter increased and blockings occurred more and more frequently. The size of the jitter and the degree of blocking also depended on the discharge rate. At a low rate the jitter was lower and the degree of blocking less than at the high rate. Injection of edrophonium could decrease or increase the jitter or leave it unaffected. Single fibre electromyography is a sensitive way of studying the transmission in individual motor end-plates in myasthenia gravis and is also a valuable diagnostic aid.


Role of extrapulmonary conversion in mediating the systemic pressor activity of angiotensin I. The effect of antibodies against angiotensin II (AII) on systemic pressor responses to intravenously injected AII and angiotensin I (AI) was studied in a group of bioassay rats. AII antibody was only 29% as effective in neutralizing AI given intravenously as it was in neutralizing AII injected by the same route. Control plasma caused no change in the relative potencies of AI and AII. In a further series of experiments, AII antibody was significantly less effective in blocking intra-arterial AI than in blocking intravenous AI. The potency of intra-arterial AI, initially less than that of intravenous AI, became nearly twice that of intravenous AI after antibody administration, a result which could not occur if AI were inactive before lung transit. Thus, AI can elicit systemic pressor activity independently of pulmonary conversion to AII. However, since the intra-arterial AI responses were abolished by an inhibitor of angiotensin-converting enzyme, the activity would appear to be mediated by peripheral conversion to AII rather than by an intrinsic action of the decapeptide. Both series of experiments suggest that the efficacy of AII antibody in abolishing the systemic pressor activity of AI is highly dependent on the site of conversion of the AI to AII. The occurrence of localized intramural conversion of AI to AII near arteriolar receptors in vivo may so minimize exposure of the liberated AII to circulating antibody as to render AII immunization an inefficient means of blocking endogenous pressor activity of the renin-angiotensin system.


Oncornavirus expression in human x mouse hybrid cells segregating mouse chromosomes. Human x mouse hybrid clones obtained by fusing transformed human (VA2) cells with embryonic mouse brain cells were tested for their ability to spontaneously express type C virus particles. It had been previously shown that these hybrid cells preferentially retained human chromosomes while mouse chromosomes were lost. The culture fluid from one cell line was found to contain type C particle markers in abundance, and typical budding C particles were observed in the cells by electron microscopy. In contrast, no particle markers were detected in the culture fluid from parental cells and several other hybrid cell lines. Subclones of the virus-positive cell line continued to lose mouse chromosomes and were found to vary more than 100-fold in their culture fluid DNA polymerase activity. The hybrid cell viruses, termed HMV1, banded in a sucrose gradient between 1.14 and 1.16 g/ml, possessed viral group-specific antigens, and exhibited B-tropic host range for replication in mouse embryo cells, but did not replicate in human cells when directly applied. The virus did not transform mouse cells but was able to rescue the defective murine sarcoma virus from sarcoma-positive, helper-virus-negative cells. Activity of the DNA polymerase associated with HMV1 was similar to the activity of Rauscher murine leukemia virus (MuLV) DNA polymerase in its preference for poly(rA) over poly(dA) as a template, use of endogenous template, detergent requirement, and inhibition by antiserum directed against MuLV.DNA polymerase. The results suggest that human x mouse hybrid cells segregating mouse chromosomes can spontaneously express endogenous type C viruses and that such hybrid cell lines may be used for the isolation of latent mammalian oncornaviruses and analysis of viral gene regulation.


Control of derepressed beta-galactosidase synthesis in Escherichia coli.


Functional and morphological effects of hypophysectomy and androgen replacement in the rabbit epididymis.


Induction and isolation of auxotrophic mutants in mammalian cells.


Isolation of temperature-sensitive mutants of mammalian cells.


Methods for obtaining revertants of transformed cells.


Uses of enucleated cells.

Uses of enucleated cells.


Ionic coupling between nonexcitable cells in culture.


Enucleation of somatic cells with cytochalasin B.


Persistent changes induced by subcarcinogenic doses of 3'-methyl-4-(dimethylamino)azobenzene in rat liver.


Methods of cell transformation by tumor viruses.


Effect of N-(3,5-dichlorophenyl)succinimide on the histological pattern and incidence of kidney tumors in rats induced by dimethylnitrosoamine.


Variation in the mode of sugar transport among normal and tumor cells.


Episodic secretion of aldosterone in primary aldosteronism: relationship to cortisol.


Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.


Ribosomal activities dependent on elongation factors T and G. Effects of methanol.


Effect of Clostridium perfringens phospholipase C(alpha-toxin) on the human diploid fibroblast membrane.


Phosphorylation of N-protected deoxyoligonucleotides by T4 polynucleotide kinase.


Conformation of nicotinamide adenine dinucleotide bound to cytoplasmic malate dehydrogenase.


Polynucleotides containing 2'-amino-2'-deoxyribose and 2'-azido-2'-deoxyribose.


Acidophilic activation of steroid hormone receptors.


Gonadotropin regulation of nucleotide biosynthesis in corpus luteum.


Studies on the function of central noradrenergic neurons.


Shape of the hydrophobic barrier of phospholipid bilayers (evidence for water penetration in biological membranes).


Regulation of glycolysis in the early development of fish embryos.


Galactosyl-diglyceride from Actinomyces viscosus.


Virus, the primordial parasite, and man.


Mass spectra of complex molecules. I. Chemical ionization of sphingolipids.


Herpesviruses, latency and cancer: a biochemical approach.


The Wilms' tumour--old and new facts. I. Clinical features and tumour biology.


The Wilms' tumour--old and new facts. 3. Special problems in therapy.


Intraoperative upper gastrointestinal endoscopy and biopsy.


Granuloma annulare and vitamin D resistant rickets.


Radioimmunoassay of pituitary gonadotropins: serum levels in normal women and those with gonadal dysfunction.


The role of estradiol in the equilibrium binding of estrogen receptor to rabbit uterine chromatin.


Effect of dibutyryl cyclic amp on glucose-6-phosphatase activity in human fetal liver explants.


Effects of decapitation, ether and pentobarbital on guanosine 3',5'-phosphate and adenosine 3',5'-phosphate levels in rat tissues.


Effect of triamcinolone treatment, starvation and diabetes on rat liver protein kinase activity.


The physical chemistry of hemes and hemopeptides. 3. Synthesis and physicochemical properties of cytochrome c-related heme--pentapeptide soluble in organic solvents.


The enrichment of adenylate cyclase in the plasma membrane and golgi subcellular fractions of porcine adenohypophysis.


Role of the cell surface in growth and transformation.


Ultrastructure, steroidogenic potential, and energy metabolism of the Snell adrenocortical carcinoma 494. A comparison with normal adrenocortical tissue. Electron microscope studies were carried out with the adrenocortical carcinoma 494 and normal adrenal cortex tissue. The mitochondria of the tumor cells showed marked differences when compared with mitochondria from fasciculata cells of the normal adrenal cortex. These differences were primarily related to mitochondrial number and crista structure. Corticosterone production in isolated tumor cells was extremely low and neither ACTH nor dibutyryl cyclic AMP had any stimulatory effect. Normal adrenal cells showed at least a tenfold increase under identical conditions. In the presence of corticosteroid precursors the amount of corticosterone produced by the tumor cells was much less than that produced by normal cells. The results indicate a reduced capacity for 11beta-hydroxylation in the tumor mitochondria and a possible reduced capacity for biosynthetic steps before the 11beta-hydroxylation reaction. Glycolysis in isolated tumor cells was also lower than in normal cells. Isolated tumor mitochondria oxidized succinate normally with a good degree of coupling with phosphorylation. However, unlike normal adrenal mitochondria, the tumor mitochondria showed little or no oxygen uptake with other Krebs cycle substrates. These data suggest that the tumor mitochondria may be lacking in the flavoprotein dehydrogenases responsible for the oxidation of NADH and NADPH, although other components of the respiratory chain may be intact.


Time on organic evolution.

Time on organic evolution.


Colchicine-induced inhibition of lipoprotein and protein secretion into the serum and lack of interference with secretion of biliary phospholipids and cholesterol by rat liver in vivo. Rats were injected with colchicine and the secretion of triglycerides into the serum was studied for 90 min after injection of [(14)C]palmitic acid and Triton WR 1339. The release of labeled and chemically determined triglyceride was reduced to about 20-30% of control values. The effect of colchicine on serum triglyceride levels was not dependent on the presence of Triton and was similar in males and females and in fed and fasted rats. The effect was dose dependent and was reversible 6-7 h after injection of 0.05 mg/100 g body weight. Colchicine inhibited also the release of labeled proteins into the serum but did not affect the amount of [(3)H]leucine incorporated into liver proteins. Within 4 h of colchicine treatment there was an 80% fall in serum very low density lipoproteins (VLDL), a 30% fall in serum high density lipoproteins (HDL), and no change in the d > 1.21 protein level, but reduction in the appearance of labeled proteins was encountered in all serum fractions. Colchicine had no effect on the rate of bile flow and on the secretion of phospholipids and cholesterol into the bile. In the hepatocyte there was accumulation of Golgi-derived secretory vesicles, containing nascent VLDL particles; these vesicles were seen also in the vicinity of the sinusoidal cell surface, but the space of Disse contained few or no VLDL particles. There was an apparent reduction in microtubules and some increase in microfilaments. It is suggested that microtubules affect the secretion of lipoproteins and proteins into the serum by maintaining the organization of the plasma membrane required for its fusion with secretory vesicles. The lack of effect of colchicine on biliary lipid secretion indicates that the latter is not dependent on vesicular transport.


Effect of dibutyryl adenosine cyclic 3',5'-monophosphate and testololactone on concanavalin A binding and cell killing.


Observations on the epidemiology of pseudocowpox in South-west England and South Wales.


A study of the renin-angiotensin system in the nephrotic syndrome.


The effect of intravenous oestrogen injections on plasma human placental lactogen levels.


Adenosine 3',5'-monophosphate in guinea pig cerebral cortical slices: effect of benzodiazepines.


Alterations in cyclic AMP metabolism associated with photoreceptor cell degeneration in the C3H mouse.


Effects of ATP on the activity of nucleoside 3',5'-cyclic monophosphate phosphodiesterase from brain.


Oat cell tumor of the small bowel.

Oat cell tumor of the small bowel.


Corticotrophin-like peptides in the rat pituitary.


Purification and characterization of porcine corticotrophin-like intermediate lobe peptide.


The thyrotrophin receptor in guinea-pig thyroid homogenate: general properties.


DNA synthesis and the secretion of prolactin and growth hormone by the pituitary gland of the male rat: effects of diethylstilboestrol and 2-bromo-alpha-ergocryptine methanesluphonate.


The thyrotrophin receptor in guinea-pig thyroid homogenate: interaction with the long-acting thyroid stimulator.


Genetic study of the effects of (8-arginine)- and (8-lysine)-vasopressins on adenyl cyclase activity of mouse kidney.


Letter: Serological tests for amoebiasis.


Virological findings and blood gas tensions in acute lower respiratory tract infections in children. Sequential blood gas tensions and pH have been measured in 84 children selected from 486 admitted to hospital during a 15-month period with acute lower respiratory tract infections. Of those selected 73 were treated conservatively and 11 by intermittent positive-pressure ventilation; one infant in the latter group died. Respiratory syncytial virus (R.S.V.) was isolated from 24 out of 62 patients studied and the main pathogen in the most severely affected infants. Statistical analysis showed that age and R.S.V. infection were independent determinants of severity, as reflected by a peak Pco(2) measurement at the height of the illness (age, P < 0.01; R.S.V. 0.05 > P > 0.01).


Acute respiratory failure in bronchiolitis and pneumonia in infancy. Modes of presentation and treatment. The modes of presentation and the management of acute respiratory failure in 11 infants with severe lower respiratory tract infections (due to respiratory syncytial virus in eight) are described. Progressive respiratory difficulties leading to exhaustion, peripheral circulatory collapse, recurrent apnoeic attacks, or generalized convulsions were the main clinical presentations resulting in severe ventilatory failure. In nine infants preventilation carbon dioxide tensions exceeded 65 mm Hg. It seems likely that the use of intermittent positive-pressure ventilation in these patients contributed to the low mortality rate, less than 0.5%, from such illnesses during the 15-month study period.


Studies on hormone-cytostatic complexes in the cytoplasm of rat prostatic gland.


Estracyt (NSC 89199) as a substrate for phosphatases in human serum.


Immunology of virus-induced tumours.

Immunology of virus-induced tumours.


Cellular hypersensitivity in Guillain-Barré syndrome. The Guillain-Barré syndrome is hypothesized to be secondary to cellular hypersensitivity to peripheral nerve antigens. To test this theory lymphocytes from 100 subjects were studied using the macrophage-migration-inhibition factor (MIF) assay. Thirty-four normal controls gave a mean migration of 100.4 +/- 9%. Of 34 patients with peripheral nervous system disease, only those with the Guillain-Barré syndrome showed hypersensitivity with a mean migration of 72 +/- 11%. Of 34 patients with central nervous system disease only three with multiple sclerosis and two with stroke gave similar results. Positive results in the Guillain-Barré syndrome were found only in patients presenting with classical disease and who were ill at the time of study.


Oncogenic DNA viruses of nonhuman primates: a review.


Transvenous adrenal destruction: clinical trials in patients with metastatic malignancy.


A possible mechanism of 1,2-shift reactions catalyzed by coenzyme B 12 dependent enzymes.


The pharmacodynamics of depression and its relation to therapy.


Beta-islet cell tumors of the pancreas: results of a study on 1,067 cases.


Contributions of clonal systems to neurobiology.


Studies on the availability of intramitochondrial carbamoylphosphate for utilization in extramitochondrial reactions in rat liver.


Cytochrome c interaction with membranes. Interaction of cytochrome c with isolated membrane fragments and purified enzymes.


Effects of membrane steroid modification on human erythrocyte glucose transport.


On the interpretation of electron paramagnetic resonance spectra of binuclear iron-sulfur proteins.


Stimulation of cytochrome synthesis in Escherichia coli by cyclic AMP.


Polyacrylamide gel electrophoresis of glycoproteins on single concentration and gradient gels.


Resonance-raman evidence for anomalous heme structures in cytochrome c' from Rhodopseudomonas palustris.


Purification and properties of a protein which stimulates succinate oxidation in the particulate fraction of Azotobacter vinelandii.


Localization of low-density lipoproteins in atherosclerotic lesions from human normolipemics employing a purified fluorescent-labeled antibody.


Energy-dependent accumulation of iron by isolated rat liver mitochondria. 3. Submitochondrial localization of the iron accumulated.


Biochemical and biophysical studies on cytochrome c oxidase. XVI. Circular dichroic study of cytochrome c oxidase and its ligand complexes.


Synaptic potentials of hypoglossal motoneurons and a common inhibitory interneuron in the trigemino-hypoglossal reflex.


Articular rhizotomy.

Articular rhizotomy.


The accumulation of adenosine 3',5'-monophosphate in cerebral cortical slices of the quaking mouse, a neurologic mutant.


Biochemical and physiological properties of a purified snake venom neurotoxin which acts presynaptically.


Characterization of the serum low-density lipoproteins of normal and two rhesus monkeys with spontaneous hyperbeta-lipoproteinemia.


Alcohol dehydrogenases of Euglena gracilis, strain Z.


A DNA antigen that reacts with antisera to cardiolipin.


Cyclic AMP stimulation of calcium efflux from kidney, liver and heart mitochondria.


Liver microsomal electron transport systems. II. The involvement of cytochrome b5 in the NADH-dependent hydroxylation of 3,4-benzpyrene by a reconstituted cytochrome P-448-containing system.


Singlet oxygen generation by the lipoxidase system.


Effect of nuclear protein kinases on mammalian RNA synthesis.


Proceedings: Effects of hypoxia on maternal and foetal renin and angiotensin II in sheep.


Proceedings: Peptide antagonists of the renin-angiotensin system and the elucidation of the receptors for angiotensin-induced drinking.


Use of (1-14C) aectic anhydride to quantitate diglycerides: a new analytical procedure.


The muscular atrophy of central origin (an ultramicroscopic study on muscle biopsy material).


The amino-acid sequence of sarcine adenylate kinase from skeletal muscle.


Positive and negative control of bacterial gene expression.


A prospective controlled study of neonatal hypoglycemia.


Performances of Mueller-Hinton agars prepared by three manufacturers.


Role of the superoxide anion in the myeloperoxidase-mediated antimicrobial system.


Proceedings: Physiology and enzymology of frog photoreceptor membranes.


Proceedings: Light regulation of adenosine 3',5' cyclic monophosphate levels in vertebrate photoreceptors.


A new overgrowth syndrome with accelerated skeletal maturation, unusual facies, and camptodactyly.


Lipoproteins in neonates.

Lipoproteins in neonates.


The effects of acetylcholine and of synaptic stimulation on the sensorimotor cortex of cats. I. Neuronal responses to stimulation of the reticular formation.


Biochemically active substances from Actinomycetes and other organisms. II. Effects of various compounds on motilometry.


Dissociated loss of cold and warm sensibility during regional anaesthesia.


Fine structure of Bowen's disease in chronic arsenicalism.


Effects of cis-dichlorodiammine platinum (II) in the regression of sarcoma 180: a fine structural study.


Humoral immune responses to tumor-specific antigens in strain-2 guinea pigs.


Summary and recommendations of the workshop on uterine-cervical cancer.


Epithelial cell cultures from normal glandular tissue of mice.


Respiratory virus inhibitors in the colostrum of Chinese donors.


Effects of corticotrophin, starvation and glucose on ascorbic acid levels in the blood plasma and liver of piglets.


Lack of fixed order of recruitment in cat motoneuron pools.


Purification of alpha-toxin (phospholipase C) of Clostridium perfringens by affinity chromatography.


Fine structure of myogenesis and elastogenesis in the developing rat lung.


A colicin-tolerant mutant of Escherichia coli with reduced levels of cyclic AMP and the regulation of lambdoid phage lysogeny.


Spin-label studies at F9(93)beta of deoxyhemoglobin S aggregation.


Brenner tumors and Walthard cell nests.

Brenner tumors and Walthard cell nests.


Atherosclerosis in rat kangaroos.

Atherosclerosis in rat kangaroos.


Proteinaceous hexagonal inclusions in hyphae of Whetzelinia sclerotiorum and Neurospora crassa.


Influence of mesencephalic stimulation on unit activity in striate cortex of squirrel monkeys.


A supraspinal monosynaptic input to hindlimb motoneurons in lumbar spinal cord of the frog, Rana catesbiana.


Dynamic characteristics of responses to horizontal head angular acceleration in vestibuloocular pathway in the cat.


Phenylethanolamine-N-methyl transferase activity in the avian adrenal following immobilization or adrenocorticotropin.


Transformation by adenovirus type 12 of rat embryol fibroblasts cultivated in the presence of interferon.


Bacterial pathogens in dry dog foods.

Bacterial pathogens in dry dog foods.


Alterations with age in composition and lipolytic activity of adipose tissue from male and female chickens.


Abnormality in hepatic lipids of a patient having taken coralgil, (hexestrol bis (beta-diethylaminoethyl ether)) for a long period.


The effect of ergocornine on melanophores of Ambystoma tigrinum: evidence for suppression of pituitary MSH release.


Gas chromatographic resolution of homologous monoacyl and monoalkylglycerols.


Subcutaneous glucagon as a test of the ability of the pituitary to secrete GH and ACTH.


Biosynthesis of monoacyl-sn-glycerol 3-phosphate by rabbit heart mitochondria: positional specificity differing from liver enzyme.


Prostaglandins of rat testis.

Prostaglandins of rat testis.


Embryonic vs tumor lipids. II. Changes in phospholipids of developing chick brain, heart, and liver.


The ACTH-enhanced conversion of 118-hydroxyprogesterone to corticosterone in the adrenal glands of fetal rhesus monkeys (Macaca mulatta).


In vivo and in vitro biosynthesis of free fatty alcohols in Escherichia coli K-12.


Gas chromatographic differentiation of 4-desmethyl, 4-monomethyl and 4,4-dimethylsterols.


Simultaneous radioimmunoassay of testosterone and dihydrotestosterone.


Extranasopharyngeal juvenile angiofibroma--a case report.


Plasticity in small neuronal assemblies. When spike activity of two or more individual neurons is simultaneously recorded, it is possible to detect various types of neuronal connections to and between the observed neurons by making appropriate statistical analysis of the data. Such methods are reviewed. This approach is particularly useful in seeking changes of neuronal connectivity associated with behavioral plasticity. Examples from experiments with Aplysia and crayfish are given.


Influence of pure virus infection on wound healing.


Insulin responses to calcium infusion.

Insulin responses to calcium infusion.


The role of the renin-angiotensin system in the aldosterone response to acute hemorrhage.


Alterations in plasma aldosterone levels in response to acute stimuli in kidney allograft recipients.


Influence of altered cyclic 3',5'-adenosine monophosphate phosphodiesterase activity on systemic hemodynamics.


Diagnosis of pulmonary cytomegalic virus by bronchoscopy.


Temperature-sensitive mutations in Habrobracon.


Histamine and collagen synthesis in keloid and hypertrophic scar.


The regulation of NAD L-glutamate dehydrogenase in Aspergillus nidulans.


Differentiation between the steroidogenic effects of cholera enterotoxin and adrenocorticotropin through use of a mutant adrenal cell line.


Demonstration of acetylcholine-sensitive adenyl cyclase in malignant neuroblastoma cells in culutre.


Yeast glycogen synthetase in the glucose 6-phosphate-dependent form. II. The effect of proteolysis.


Hydroxyproline in the early diagnosis of bone metastases in prostatic cancer.


Letter: Ménétrier disease.

Letter: Ménétrier disease.


Effects of phorbol myristate acetate on the metabolism and ultrastructure of neutrophils in chronic granulomatous disease. Previous investigations have demonstrated that phorbol myristate acetate (PMA), the active principle of croton oil, stimulates alterations in normal polymorphonuclear leukocytes (PMN) that resemble closely the changes that develop in the cells after phagocytosis of bacteria. The present study has compared the effects of PMA and heat-killed bacteria on the oxygen uptake, glucose oxidation, nitroblue tetrazolium (NBT) reduction, and ultrastructure of normal neutrophils and PMN from six patients with chronic granulomatous disease (CGD). PMA stimulated oxygen consumption, hexose monophosphate shunt activity, and NBT reduction in normal cells but failed to produce similar effects in CGD neutrophils. However, PMA did induce formation of cytoplasmic vacuoles in the CGD cells similar to those observed in normal neutrophils. The results indicate that PMA is a useful nonparticulate agent for distinguishing between normal and CGD neutrophils and for studying basic mechanisms of phagocytosis in normal and abnormal PMN.


Sensitivity of serological tests and the diagnosis of streptococcal sore throat in children.


Proceedings: Renin and lysosomal enzyme activities in the kidney of spontaneously hypertensive rats.


Regulation of glycerol catabolism in Klebsiella aerogenes. The utilization of glycerol as a carbon source for growth by Klebsiella aerogenes, strain 2103, involves separate aerobic (sn-glycerol-3-phosphate or G3P) and anaerobic (dihydroxyacetone or DHA) pathways of catabolism. Enzyme and transport activities of the aerobic pathway are elevated in cells grown under oxygenated conditions on glycerol or G3P. Anaerobic growth on G3P as carbon source requires the presence of an exogenous hydrogen acceptor such as fumarate; cells thus grown also are highly induced in the G3P pathway. Anaerobic growth on glycerol requires no exogenous hydrogen acceptors; cells thus grown are highly induced in the DHA pathway but almost uninduced in the G3P pathway and the addition of fumarate electron acceptors has no effect on the relative levels of the two pathways. When both glycerol and G3P are provided anaerobically with fumarate, the DHA pathway is still preferentially induced, which probably accounts for the exclusive utilization of glycerol until its exhaustion. These observations suggest the presence of a regulatory control of G3P pathway imposed by the operation of the DHA pathway.


Evidence for a composite state of an F'his, gnd element and a cryptic plasmid in a derivative of Salmonella typhimurium LT2. A method designed to select mutants constitutive for expression of the histidine operon has been applied to a Salmonella typhimurium LT2 strain containing an F'his,gnd element and a cryptic plasmid. One of the mutants isolated, strain AA0019, has not only increased levels of histidinol phosphate phosphatase (hisB), but also increased levels of gluconate-6-phosphate dehydrogenase (gnd). Ultracentrifugation studies of extrachromosomal deoxyribonucleic acid (DNA) isolated from strain AA0019 revealed the presence of a single species of covalently closed circular (CCC) DNA that sedimented more rapidly through neutral and alkaline sucrose gradients than any of its possible plasmid precursors. From neutral sucrose gradients, sedimentation coefficients of 130, 100, and 86S were derived, corresponding to the CCC DNA of the large plasmid in strain AA0019, the F'his,gnd element and the cryptic LT2 plasmid, respectively. An Escherichia coli plasmid-free strain that upon mating had received the large 130S plasmid also contained 86S and 100S CCC DNA components. A histidine-requiring derivative of strain AA0019 obtained after acridine orange treatment retained the cryptic plasmid DNA. Apparently, the large plasmid in strain AA0019 consists of the F'his,gnd element and the cryptic LT2 plasmid of the parental strain.


Differential diagnosis of an abnormal brain scan.


Papillary cystadenoma lymphomatosum associated with pleomorphic adenoma of the parotid gland.


Reusability of silica gel in thin layer chromatography.


The effects of human serum on local (Ibadan) strains of Entamoeba histolytica. (Preliminary studies).


Proceedings: Long-term follow-up of fluorine-18 bone scans in carcinoma of the prostate.


Proceedings: Urinary hydroxyproline and leucocyte ascorbic acid levels in patients bearing breast tumour with skeletal metastases.


Studies on a new antiviral antibiotic, 9-methylstreptimidone. I. Physicochemical and biological properties.


Juvenile nasopharyngeal angiofibroma.

Juvenile nasopharyngeal angiofibroma.


Formation of interchain disulfide bonds and helical structure during biosynthesis of procollagen by embryonic tendon cells.


Comparison of proteins of ribosomal subunits and nucleolar preribosomal particles from Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.


An electrophoretic method for the quantitative isolation of human and swine plasma lipoproteins.


Wilms' tumor.

Wilms' tumor.


Adrenergic receptors and insulin secretion.


Fat absorption after infusing bile salts into the human small intestine.


Electron microscopy of small intestinal mucosa in pancreatic insufficiency.


Jejunal calcium transport in health and metabolic bone disease: Effect of vitamin D.


Changes in the fine structure of mouse gastric epithelium produced by ethanol and urea.


Pathogenesis of herpetic encephalitis in mice after ophthalmic inoculation.


News. From the National Academy of Sciences: Nomenclature of antigens associated with viral hepatitis type B.


Effect of adenine arabinoside on cytomegalovirus infections.


Hyperoxia and lung metabolism.

Hyperoxia and lung metabolism.


Clinical trial design in studies of corticosteroids for bacterial infections.


Letter: Anticoagulant therapy and acute adrenal insufficiency.


Viral disease of the labyrinth. I. Review of the literature and discussion of the role of cytomegalovirus in congenital deafness.


Letter: Androgen role in erythroleukemia after treatment with alkylating agents.


Use of amoxicillin in the treatment of patients with septic abortion.


Ribonuclease T1 catalyzed degradation of transfer RNA: an unusual alteration induced by urea.


The frog dorsal column nucleus.

The frog dorsal column nucleus.


Evaluation of the role of 5-phosphoribosyl-alpha-1-pyrophosphate synthetase in congenital hyperuricemia and gout: a simple isotopic assay and an activity stain for the enzyme.


A theoretical treatment of the turnover of protein-bound phosphate in the presence of both protein kinase and phosphatase activities.


The effect of hypophysial hormones and human chorionic gonadotrophin (HCG) on the anencephalic fetal adrenal cortex and on parturition in the human.


Uptake and relase of (14C)adenine derivatives at beds of mammalian cortical synaptosomes in a superfusion system.


Transfer RNA and the regulation of protein synthesis in rat cerebral cortex during neural development.


Comparison of effects of long-term corticotrophin and corticosteroid treatment on responses of plasma growth hormone, ACTH, and corticosteroid to hypoglycaemia. The development of the highly sensitive cytochemical bioassay for ACTH has permitted the measurement of plasma ACTH levels during the insulin hypoglycaemia test (I.H.T.) in patients treated with corticosteroids and corticotrophin. The ACTH, corticosteroid, and growth hormone (GH) responses in the I.H.T. were measured in three groups of 12 rheumatoid arthritis patients. One group was receiving long-term corticotrophin treatment, the second was undergoing long-term corticosteroid treatment, and the third had never received systemic hormone therapy. The increments in plasma ACTH, corticosteroids, and GH were diminished in the corticosteroid-treated group, as were increments in plasma GH and ACTH in the corticotrophin-treated group; but in this group the corticosteroid increment was normal. Examination of the area under the curve of the ACTH response showed that the total amount of ACTH secreted was normal though the rate of secretion was reduced. In the corticosteroid-treated group both rate and total secretion were diminished.


Methods for selecting and studying temperature-sensitive mutants of BHK-21 cells.


Reversal of bacteriophage T4 induced polynucleotide kinase action.


The catecholamines and aggressive behavior.


Experimental herpes infection in the newborn rat. 3. Disease development after oral inoculation.


Conjunctival collagenase: partial purification.


The pathogenesis of the neuropathy in dimorphous leprosy: electron microscopic and cytochemical studies.


Thyrotrophin responses to intravenous thyrotrophin-releasing hormone in patients with hypothalamic and pituitary disease.


Glycerolipid formation from sn-glycerol-3-phosphate by rat liver cell fractions. The role of phosphatidate phosphohydrolase.


31P nuclear magnetic resonance: application to the study of human serum high density lipoproteins.


Effect of exogenous adenosine cyclic 3'-5'-monophosphate on antibody-producing cells in vivo and in vitro.


An enzymatic explanation for dietary induced alterations in hepatic glycerolipid metabolism.


Transcription products synthesized by purified calf thymus DNA-dependent RNA polymerase on Shope papilloma virus DNA.


Virus-like particles in human laryngeal papilloma. An ultrastructural study.


The efficacy of various mutagens and polyene antibiotics for the induction and isolation of sterol mutants of Neurospora crassa.


Chemical induction of streptomycin-resistant mutations in Escherichia coli. Dose and mutagenic effects of dichlorvos and methyl methanesulfonate.


Methionine biosynthesis in Saccharomyces cerevisiae: mutations at the regulatory locus ETH2. I. Genetic data.


Rsf mutants of Escherichia coli HfrC defective in the production of the factor stimulating recombination in conjugation.


Repair replication and unscheduled DNA synthesis in mammalian cell lines showing differential sensitivity to alkylating agents.


The molecular biology of the steroid hormones.


Effect of prostaglandin E1 on hepatic cyclic AMP activity, carbohydrate and lipid metabolism.


Effects of oestradiol on different cell types.


Sequential gene expression in response to estradiol-17 beta during post-natal development of rat uterus.


The relationship of transmitter release and storage to fine structure in a sympathetic ganglion.


Medical management of the patient with arthritis.


Tibial osteotomy for the treatment of degenerative arthritis of the knee.


The substantia gelatinosa Rolandi of the rat. Fine structure, cytochemistry (acid phosphatase) and changes after dorsal root section.


Toxicity of collagenase: the relation to enzyme therapy of disk herniation.


Effect of a surgical horizontal unidirectional filtered air flow unit on wound bacterial contamination and wound healing.


Stabilization of pathologic fractures with acrylic cement.


Application of the cytochemical bioassay for corticotrophin to clinical and physiological studies in man.


Studies on the mechanism of cytochemical bioassays.


Cytomegalic inclusion syndrome with thrombocytopenia.


Lanthanum inhibition of the action of oxytocin on the water permeability of the frog urinary bladder: effect on the serosal and the apical membrane.


On the Nordic Pharmacopoeia limit test for heavy metals. Heating with anhydrous sodium sulphate as a source of error.


Principles of synaptic ultrastructure in the central nervous system.


The effect of chlorhexidine on some biochemical parameters of rat liver microsomes.


Serial plasma growth hormone concentration during selective removal of pituitary tumors in acromegaly.


Lymphocyte inhibition by mammary tumor virus in patients with breast cancer.


Collagenase activity in the alkali-burned cornea.


Experimental liquefaction of the vitreous body.


Functional morphology of cestode larvae.


Close genetic linkage of the determinants of the ribitol and D-arabitol catabolic pathways in Klebsiella aerogenes. Klebsiella aerogenes strain W70 has separate inducible pathways for the degradation of the pentitols ribitol and d-arabitol. These pathways are closely linked genetically as determined by transduction with phage PW52. There are two regulatory sites for the ribitol catabolic pathway as defined by loci for mutations to constitutive synthesis of ribitol dehydrogenase and d-ribulokinase, rbtB and rbtC. The two control sites are separated by a site represented by the dalB22 mutation. This mutation deprives the cell of the ability to induce synthesis of d-arabitol dehydrogenase and d-xylulokinase activities. Two additional regulatory mutations for the d-arabitol pathway, dalC31 and dalC37, map to the opposite side of rbtB13 relative to dalB22. The order of the genetic sites thus far determined for this region is dalK-dalD-dalC31, dalC37-rbtB13-dalB22-rbtC14-rbtD-rbtK, where dalK and dalD represent structural genes for the kinase and dehydrogenase of the d-arabitol pathway, respectively, and rbtK and rbtD represent the corresponding genes for the ribitol pathway. The two mutations that lead to constitutive synthesis of the d-arabitol-induced enzymes, dalC31 and dalC37, have different phenotypes with regard to their response to xylitol. The growth of dalC31 is inhibited by xylitol, but the toxicity can be reduced by increasing the levels of ribitol dehydrogenase either by induction with ribitol or by selection of a ribitol dehydrogenase-constitutive mutation.


Bladder function during lumbar myelography. An experimental investigation in cats.


Low temperature photodissociation of hemoproteins: oxygenated cobalt-myoglobin and hemoglobin.


Cyclic nucleotide phosphodiesterase activities from isolated fat cells: correlation of subcellular distribution with effects of nucleotides and insulin.


Direct evidence for the involvement of epoxide intermediates in the biosynthesis of the C18 family of cutin acids.


Divalent metal ion effects on a mutant histidinol phosphate phosphatase from Salmonella typhimurium.


Characterization of porcine malate dehydrogenase. II. Amino acid sequence of a peptide containing the active center histidine residue.


Chimpanzee (Pan troglodytes) serum high density lipoproteins. Isolation and properties of their two major apolipoproteins.


Biochemical and biophysical studies on cytochrome c oxidase. XVII. An epr study of the photodissociation of cytochrome a32+-CO.


Effects of ATP, antimycin and cyanide on the EPR spectra of cytochromes in phosphorylating submitochondrial particles.


A re-examination of succinic dehydrogenase activity and its association with cell death in the interdigit of the chick foot.


Application of physical methods to the study of serum lipoproteins.


Half-site reactivity.

Half-site reactivity.


The role of the membrane in the utilization of nucleic acid precursors.


Use of Xavin in auditory nerve lesions.

Use of Xavin in auditory nerve lesions.


The inhibition of alkaline phosphatase by periodate and permanganate.


Angiotensin I-converting enzyme of the kidney cortex.


Complications in therapeutic soft lens wear.


Intracellular localisation and properties of aldehyde dehydrogenases from sheep liver.


Maleimideinactivation of lactate dehydrogenase isozymes.


Inhibitor of nitrate reductase in the roots of rice seedlings and its effect on the enzyme activity in the presence of NADH.


Kinetics of amidophosphoribosyltransferase in intact tumor cells.


Partial purification and characterization of an adenosine 3',5'-monophosphate-dependent protein kinase from rabbit gastric mucosa.


Activation and phosphorylation of carbonic anhydrase by adenosine 3',5'-monophosphate-dependent protein kinases.


Stimulation by thyrotropin of horse thyroid plasma membranes adenylate cyclase: evidence of cooperativity.


Isolation of cyclic inositol-1,2-phosphate from mammalian cells and a probable function of phosphatidylinositol turnover.


Isoenzymes of sphingomyelinase and the genetic defect in Niemann-Pick disease, type C.


Lecithin biosynthesis in liver mitochondrial fractions.


In vitro triiodothyronine binding to non-histone proteins from rat liver nuclei.


Pulmonary carcinoma (Jaagsiekte) of sheep: pathologic findings and comparison in multiple-case and case-free herds.


Comparative studies on some herpes simplex virus strains with different locations. 3. Cultivability in cell cultures.


Gene manipulation: progress and prospects.


Histologic differences in breast carcinoma of Japanese and American women.


Lung cancer at autopsy in a-bomb survivors and controls, Hiroshima and Nagasaki, 1961-1970. I. Autopsy findings and relation to radiation.


Hereditary tumors of the carotid bodies and chronic obstructive pulmonary disease.


Estimation of the mean and variance of cycle times in cinemicrographically recorded cell populations during balanced exponential growth.


Biosynthesis of yeast mitochondria. Some effects of ethidium bromide on Kluyveromyces (Saccharomyces) fragilis.


Effects of indomethacin on Moloney sarcoma virus-induced tumors.


The study and effect of streptoxotocin (NSC-37917) rendered diabetic Chinese.


Ultrastructure of the tegument of Schistosoma.


Virologic and serologic studies on infectious mononucleosis-like disease in children, with special reference to cytomegalovirus, Epstein-Barr virus and adenovirus infections.


Effect of alpha-methyldopa and of spironolactone on angiotensin responsiveness in man.


Current evaluation research on mental health services.


Ego defense patterns in manic-depressive illness.


Intrinsic urethral peristalsis.

Intrinsic urethral peristalsis.


The Nader report: one author's perspective.


Genetic influence on development of reticulum cell sarcomas in SJL-J mice.


Ultrastructure and function of the mesenchyme of the rat adrenal cortex.


Recurrent sciatica associated with herpes simplex. Case report.


Symmetrical transcription in animal cells and viruses.


Early changes of protein-kinase (glycogen-synthetase kinase) in denervated muscle.


Erythema multiforme exudativum (Stevens-Johnson syndrome) following sodium diphenylhydantoin therapy.


Complementation in theta-toxin production between mutants of two groups of Clostridium perfringens.


Effects of short-term ozone exposure on lung mitochondrial oxidative and energy metabolism.


Transmitter sensitivities of some nerve and muscle cells in culture.


Viral infection and lipemia: a problem of disordered host physiology.


Short-term toxicity of pentachlorophenol in rats.


Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype. Forty-eight temperature-sensitive (ts) mutants have been isolated from a wild-type strain of the New Jersey serotype of vesicular stomatitis virus (VSV) after exposure to the base analogue mutagen 5-fluorouracil. Of these mutants, 47 have been classified into 6 nonoverlapping complementation groups containing 21, 17, 4, 3, 2, and 1 mutant, respectively (1 mutant remaining unallocated). The ribonucleic acid (RNA) phenotype of 23 of these mutants has been established. Four of the six groups contain one or more mutants unable to synthesize detectable amounts of viral RNA under restrictive conditions (39 C). No complementation was observed in mixed infection with ts mutants from the five established complementation groups of the Indiana serotype of VSV.


Nucleotide composition of the ribonucleic acid of rabies virus. The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus.


Reinitiation within one cell cycle of the deoxyribonucleic acid synthesis induced by simian virus 40. The infection of secondary cultures of Chinese hamster cells with simian virus 40 (SV40) induces the appearance of cells with polyploid deoxyribonucleic acid (DNA) content or chromosomal component within one cell generation. The mechanism of this phenomenon was studied by the use of 5-bromodeoxyuridine (BUdR) incorporation as a DNA density marker. When cultures were treated with (14)C-BUdR and colcemide and harvested at 48 hr postinfection, only hybrid and light DNA molecules were found in control cultures, whereas in infected cultures there were also heavy molecules. The proportion of heavy DNA synthesized during the experimental period varied from 13 to 25%. It was determined by DNA-DNA hybridization that the heavy DNA consisted of cellular DNA. In radioautographic experiments, it was shown that, under the conditions used, a fraction of the infected cell population twice replicated its complete DNA content. Analysis of the kinetics indicated that the heavy DNA resulted from the reinitiation of DNA synthesis after the initial replication of the entire cell DNA. It was concluded that, after infection with SV40, a fraction of the Chinese hamster cell population undergoes two cycles of DNA synthesis without intervening mitosis.


Different properties of synapses between a single sensory neurone and two different motor cells in the leech C.N.S. In leech ganglia, an individual sensory cell that responds specifically to noxious mechanical stimulation of the skin (N cell) excites two different motoneurones. One raises the annuli of the skin into ridges (the AE cell), while the other innervates logitudinal muscles and thereby shortens the body segment (L cell). A comparison has been made of the way in which these two synapses behave when their common presynaptic cell is stimulated in various conditions.1. Using previously described criteria, N sensory cells have been shown to make monosynaptic chemical connexions with both the AE and L motoneurones (Nicholls & Purves, 1972). Following a single stimulus, the excitatory synaptic potential recorded in the AE motoneurone was only about one tenth the size of that in the L cell (approximately 0.5 mV compared to 5 mV). Trains of impulses in the same N sensory cell gave rise to synaptic potentials in the AE and the L motoneurones that underwent phases of facilitation and depression; the facilitation, however, was characteristically greater and longer lasting at synapses upon the AE motoneurone.2. The differences between the two synapses were accentuated in Ringer fluid containing increased concentrations of Ca and also in the cold. Under both of these conditions repetitive firing by the N sensory cell could give rise to synaptic potentials in the AE motoneurone which progressively increased in amplitude, while those in the L motoneurone became smaller.3. The results suggest that the differences in synaptic transmission can be accounted for by variations in the amount of transmitter released at the presynaptic N cell terminals, rather than by differences in the post-synaptic cells. The animal's behaviour corresponds to expectations from the physiology of the synapses.


The accumulative properties of facilitation at crayfish neuromuscular synapses. 1. Synaptic facilitation was measured with intracellular recording at two classes of neuromuscular synapses in the opener muscle of the crayfish dactyl by placing a test stimulus at various intervals after either a single conditioning stimulus or a short conditioning train.2. The facilitative effect of one stimulus reaches approximately the same level with both the superficial central and superficial distal synapses. The facilitation decreases smoothly in two phases after the conditioning stimulus at superficial central synapses and in a more complex fashion at superficial distal synapses.3. The two synaptic types differ in the manner in which they add up the facilitative effects produced by each of the stimuli in a short train. With superficial distal synapses the facilitative effects of conditioning stimuli add linearly, while with superficial central synapses the facilitative effects accumulate exponentially.4. The linear addition of facilitation at superficial distal synapses is not altered when the quantal content is lowered by decreasing the external Ca concentration from 13.5 to 3 mM.5. The rate of decay of facilitation is the same following both one and three conditioning stimuli, even though the facilitation is nearly six times larger in the latter case.6. The results are discussed in terms of mechanisms for synaptic facilitation.


Quantitative and qualitative dynamics of the epidermal and cellular inflammatory reaction in primary toxic and allergic dinitrochlorobenzene contact dermatitis in guinea pigs.


Quantitative determination of the conformation of cyclic 3',5'-adenosine monophosphate in solution using lanthanide ions as nuclear magnetic resonance probes.


Rapid viscosimetric evaluation of collagenose activity.