Actions of the selective inhibitor of cholinesterase tetramonoisopropyl pyrophosphortetramide on the rat phrenic nerve-diaphragm preparation. 1. Tetramonoisopropyl pyrophosphortetramide (iso-OMPA) added for 15 min to the rat isolated phrenic nerve-diaphragm in a concentration of 30 muM, produced a complete selective and stable inhibition of cholinesterase. A concentration of 3 muM produced near complete inhibition of cholinesterase, and a concentration of 300 muM also inhibited acetylcholinesterase marginally.2. Inhibition of cholinesterase was associated with a sustained increase in the neuromuscular blocking action of exogenous butyrylcholine but not of exogenous acetylcholine. Iso-OMPA, 300 muM, in addition caused transient increases in the sensitivity of the rat diaphragm to exogenous acetylcholine and butyrylcholine. In the same concentration, it had a curare-like action on the frog rectus abdominis muscle preparation.3. Iso-OMPA, 30 muM, caused reversible increases in the amplitude of the twitch response and tetanic responses, which were of a similar magnitude in the indirectly stimulated preparation and the directly stimulated curarized preparation. Caffeine had a similar effect on the twitch response and its effectiveness was increased by iso-OMPA, and vice-versa. Amongst anticholinesterases, octamethyl pyrophosphortetramide and tetraethylpyrophosphate also enhanced the amplitude of the tetanic response, but paraoxon, dyflos, and mipafox did not.4. It is concluded that iso-OMPA, in concentrations (3 and 30 muM) which in 15 min give near maximal or maximal selective inhibition of cholinesterase, has no effect on the transmission of nerve impulses at the neuromuscular junction, but enhances reversibly the amplitude of the contractile response to stimulation by a direct action upon the muscle fibre, which involves a mechanism related to but not identical with that by which caffeine potentiates twitch tension. In higher concentrations, iso-OMPA has a curare-like action at the neuromuscular junction.


Isozyme patterns of branched-chain amino acid transaminase in cultured rat hepatocytes.


Effect of p-hydroxyacetanilide on liver cancer induction by N hydroxy-N-2-fluorenylacetamide.


Rapid uptake of dietary cholesterol by hyperplastic liver nodules and primary hepatomas.


Tumor-specific transplantation antigens in reticulum cell sarcomas and lymphomas induced by the friend virus complex.


Estimation of the association constant of the estrogen-receptor complex in human breast cancer.


Transport and metabolism of glucosamine by cultured Novikoff rat hepatoma cells and effects on nucleotide pools.


1'-Hydroxysafrole, a proximate carcinogenic metabolite of safrole in the rat and mouse.


In vivo effects of proteases on cell surface architecture and cell proliferation in the liver.


Demonstration of common sarcoma-associated antigen(s) in an established human neurogenic sarcoma cell line.


Enhancement of viral oncogenicity by the prior administration of dimethyl sulfoxide.


The establishment of MDMS-resistant cloned cell lines from in vitro cultured, MDMS-sensitive, Yoshida sarcoma cells.


The effects of chemical irritants and tobacco smoke condensate on the chorioallantoic membrane of the fertile hen's egg.


Guidelines for the control of equine viral infections.


The inhibitory action of  -aminobutyric acid, a probable synaptic transmitter.


Fluid secretory response of bovine Thiry jejunal fistula to enterotoxin of Clostridium perfringens. The effect of enterotoxin of Clostridium perfringens type A was studied in Thiry fistula of the bovine jejunum. Accumulation of fluid as a pathological response was demonstrable within 30 min after introduction of enterotoxin into the fistula; the fluid volume increased rapidly within the first 2 hr and reached maximum in 7 hr. The enterotoxin was not destroyed by the jejunal fluid; 52% of it was absorbed in 2 to 3 hr, and all of the enterotoxin was taken up within 8 hr postinoculation. The accumulation of fluid by the fistula after 0.5 to 2 hr of exposure to enterotoxin ceased completely within 5 hr.


Comparative densities of hydrolase-containing granules from normal and BCG-induced alveolar macrophages. Compared to cells from normal rabbit lungs, BCG-induced alveolar macrophages have a marked increase in hydrolase levels and the number of large electron-dense subcellular structures. This study was performed to investigate the possibility that these electron-dense structures were responsible for the increased hydrolase levels of these cells. Using sucrose gradient centrifugation, nuclei-free homogenates of normal and BCG-induced macrophages were analyzed with respect to the subcellular particles they contain. Gradient fractions were assayed for enzymes commonly associated with lysosomes as well as the mitochondrial cytochrome oxidase. Fractions of peak hydrolase activity from the BCG-induced preparations were consistently more dense than those from the normal cell preparations. Ultrastructural studies of the particulate material found in fractions of peak hydrolase activity from BCG-induced preparations revealed the presence of electron-dense, often dumbbell-shaped, granules. The data suggest that these peculiar granules are responsible for the elevated hydrolase levels of BCG-induced alveolar macrophages.


Projection of forelimb group I muscle afferents to the cat cerebral cortex.


Physiological pathways through the vestibular nuclei.


Tetrodotoxin, saxitoxin, and related substances: their applications in neurobiology.


Purification and properties of  -lactamase of pseudomonas aeruginosa.


Whooping cough in northern Israel.

Whooping cough in northern Israel.


Proton nuclear magnetic resonance and electron spin resonance investigation of the electronic structure and magnetic properties of synthetic low-spin ferric porphyrins.


Conformation of angiotensin II in aqueous solution. Evidence for the  -turn model.


Proton nuclear magnetic resonance and electron spin resonance investigation of axial solvation in planar, low-spin cobalt(II) porphyrin complexes.


Synthesis and secretion of growth hormone in the rat anterior pituitary. II. Properties of the isolated growth hormone storage granules.


Kinetics of unfolding and refolding of proteins. II. Results for cytochrome c.


Kinetics of unfolding and refolding of proteins. 3. Results for lysozyme.


DNA replication of SV40-infected cells. VII. Formation of SV40 catenated and circular dimers.


Nucleotide sequences from messenger RNA transcribed from the operator-proximal portion of the tryptophan operon of Escherichia coli.


Characterization of Simian virus 40 DNA component II during viral DNA replication.


The effect of low concentrations of L-glutamate and L-aspartate on transmitter release at the locust excitatory nerve-muscle synapse.


The modification of bulbo-spinal facilitation of the monosynaptic reflex by 5-hydroxytryptamine precursors and antagonists.


The cerebellum as a computer: patterns in space and time.


Estimation of primary sequence homology from amino acid composition of evolutionary related proteins.


Hyperbaric medicine in the US Air Force.


Vascular catheter protection and storage devices.


Implantation and use of a subcutaneous culture chamber in laboratory animals.


A purified gel diet for guinea pigs.

A purified gel diet for guinea pigs.


Intestinal microsporidiosis in Callicebus moloch.


Intraspecies variation in serum cholesterol levels in imported Macaca nemestrina.


The surgical preparation and maintenance of pigs in biovailability studies.


Housing for aquatic animals.

Housing for aquatic animals.


Cardiac puncture in laboratory animals.

Cardiac puncture in laboratory animals.


Bordetella bronchiseptica in laboratory cats from central California.


Staphylococcal botryomycosis in a specific-pathogen-free mouse colony.


Spontaneous occurrence and experimental transmission of the fungus, Fonsecaea pedrosoi, in the marine toad, Bufo marinus.


Postnatal development of the prairie vole, Microtus ochrogaster.


Comparison of hematologic values from peripheral blood of the ear and venous blood of infant baboons (Papio cynocephalus).


Maintaining frugivorous phyllostomatid bats in the laboratory: Phyllostomus, Artibeus, and Sturnira.


Experimental rubella virus infection of marmosets (Saguinus species).


Niclosamide as a treatment for Hymenolepis nana and Hymenolepis diminuta in rats.


Influence of acclimation upon rectal temperatures of rabbits subjected to controlled environmental conditions.


Outdoor housing of nonhuman primates.

Outdoor housing of nonhuman primates.


Intraocular pressure in the juvenile rhesus monkey (Macaca mulatta) by applanation tonometry. Preliminary report.


The use of fetal animals to investigate renal development.


Experimental kidney transplantation.

Experimental kidney transplantation.


Chemodectomas of the nasopharynx. An unusual case associated with unilateral ocular proptosis.


Malignant insulinoma with hepatic secondaries treated with hepatic artery ligation.


Respiratory infection which requires surgical management.


Hepatic ultrastructure in endotoxemia, hemorrhage, and hypoxia: emphasis on mitochondrial changes.


The range of carcinogenic substances in human foods and the problems of testing for them.


Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus".


R factors identified in Paris, some conferring gentamicin resistance, constitute a new compatibility group.


The effect of electro-convulsive therapy on the urinary excretion of adenosine 3':5'-cyclic monophosphate.


The effect of glucagon on adenosine 3':5'-cyclic monophosphate concentrations in an organ culture of adult rat liver.


Micro methods of osmometry based on the equilibrium swelling of gels.


Mechanisms for the control of enzymic rates.


Binding of rat liver and hepatoma polyribosomes to stripped rough endoplasmic reticulum in vitro. Biological or an artifact? Exposed thiol groups do not appear to be related to the binding of (32)P-labelled polyribosomes to stripped rough endoplasmic reticulum in vitro. Treating stripped rough endoplasmic reticulum with GSSG did not diminish binding of polyribosomes, suggesting that binding in vitro has no correlation with the inhibition of protein synthesis in vitro reported by Kosower et al. (1971). Thiol reagents, which are known to dissociate ribosomes, did not significantly decrease binding of (32)P-labelled polyribosomes to stripped rough endoplasmic reticulum. Denaturing the protein of (32)P-labelled polyribosomes or stripped rough endoplasmic reticulum of liver or hepatoma with heat, trichloroacetic acid, or HClO(4) did not alter the binding in vitro. Therefore, the practice of measuring the binding of (32)P-labelled polyribosomes to stripped rough endoplasmic reticulum in vitro (Shires et al., 1971b) is an unsuitable indicator of biological significance in the intact cell.


Structure and function of liver fructose 1,6-diphosphatase.


Localization of D-myoinositol 1:2-cyclic phosphate 2-phosphohydrolase in rat kidney. 1. On subcellular fractionation of rat kidney homogenates by differential and density-gradient centrifugation, the bulk of the inositol 1:2-cyclic phosphate 2-phosphohydrolase activity remains with the alkaline phosphatase activity, suggesting localization in the brush borders of the proximal tubules. 2. Histochemical studies with a medium containing inositol 1:2-cyclic phosphate and Escherichia coli phosphomonoesterase show Gomori staining around the brush borders of the proximal tubules in the outer cortex only. 3. Serial sections across the kidney from cortex perimeter to papilla suggest that the inositol 1:2-cyclic phosphate 2-phosphohydrolase has a limited distribution along the proximal tubule of the nephron, probably being limited to the pars convoluta, whereas the alkaline phosphatase extends along the pars recta.


Preliminary investigations on bovine hepatic fructose 1,6-diphosphatase.


Spin-spin interaction between molybdenum and one of the iron-sulphur systems of xanthine oxidase and its relevance to the enzymic mechanism. 1. Electron-paramagnetic-resonance (e.p.r.) studies at 9 and 35GHz at helium temperatures have given new information relating to the structure and mechanism of action of xanthine oxidase. 2. As reported by others, the enzyme gives two types of e.p.r. signal attributed to iron-sulphur systems. The first has g(av.)=1.95. Parameters of the second are determined as g(1) 2.12, g(2) 2.007 and g(3) 1.91, with g(av.)=2.01. This species seems to have a slightly higher redox potential than the former one. 3. Temperature-dependent changes in the form of Mo(v) e.p.r. signals from the enzyme, observed under certain conditions, are shown to be due to weak spin-spin interaction between Mo(v) and g(av.)=1.95 Fe/S. The phenomenon has been studied most fully for the Slow Mo(v) signal. Here, the spectral change takes the form of an additional approximately isotropic 11G splitting, detected below about 45 degrees K only. Samples without Fe/S reduced showed no such changes of spectrum. 4. Similar spectral changes were observed in the Rapid Mo(v) signals, obtained in rapid-freezing experiments, but only in samples corresponding to relatively long reaction times with the substrate. It is suggested therefore that the phenomenon may provide a means of distinguishing enzyme centres with Mo only reduced from those in which both Mo and Fe/S are reduced. 5. Additional rapid-freezing data tending to support a two- rather than a one-electron transfer of reducing equivalents from substrates to xanthine oxidase are reported.


Some properties of guinea-pig liver fructose 1,6-diphosphatase in relation to the control of gluconeogenesis.


The development of some enzymes of gluconeogenesis in the liver and kidney of the foetal guinea pig.


The effect of oxygen concentration on the growth and metabolism of Saccharomyces cerevisiae grown with excess of potassium or in potassium-deficient media. 1. Saccharomyces cerevisiae cells grown in limiting K(+) concentration have their growth inhibited by O(2) concentrations above 40%. With these conditions the cells grow very large and are unable to maintain ionic gradients when washed with water. 2. Cells grown in excess of K(+) showed the same pattern of change in cell size with change in O(2) concentration, but the magnitude of the changes was much less. Cells grown in excess of K(+) were not leaky. 3. Cell death, growth and development of ;leakiness' were not correlated in the cells grown in limiting K(+) concentration. 4. The activities of both alcohol dehydrogenase and cytochrome oxidase were higher in K(+)-deficient cells than in the cells grown with excess of K(+). The differences were much larger when the measurements were made on a cellular basis than when made on a protein basis. 5. In 100% O(2) 3mm-K(+) in the medium was sufficient to produce normal yeast cells.


D- and L-lactate dehydrogenases of Pseudomonas aeruginosa.


Metabolism of piperonylate by Pseudomonas fluorescens.


The effect of local anaesthetics on lipolysis in fat-cells.


Influence of a 17 beta-sulphate group on reduction of a steroid 3-oxo group by cortisone reductase.


A major influence of a 1 alpha, 2 alpha-methylene group on reduction of a steroid 3-oxo group by 2 nicotinamide-adenine dinucleotide-linked 3-hydroxy steroid oxidoreductases.


Functional groups in the catalysis and regulation of Escherichia coli citrate synthase.


The regulation of Acinetobacter sp. alpha-oxoglutarate dehydrogenase complex.


Some properties of yeast isocitrate dehydrogenase.


Activation and phosphorylation of the subunits of phosphorylase kinase.


Temporal aspects of glucose metabolism in the rat at birth.


Increased phospholipid depletion alters the apparent specificity of phospholipid-dependence of glucose 6-phosphatase activity.


Rabbit heart valve basement membrane: low platelet reactivity.


Mitochondria and respiratory enzymes of L cells treated with actinomycin D.


Surface alterations in transformed epithelial and fibroblastic cells in culture: a disturbance of membrane degradation versus biosynthesis?


Synthesis of serum proteins by a clonal strain of rat hepatoma cells.


The synthesis of surface coat materials in normal and transformed cells.


Animalizing ability of Evans blue in embryos of Arbacia punctulata. Effect on multiple molecular forms of NAD-L-malate dehydrogenase.


Ultrastructural study of the action of aminopiperazines on transformed cell lines and on nervous system tissue culture.


Cleavage inhibition in marine eggs by puromycin and 6-dimethylaminopurine.


Studies on cyclic AMP levels and phosphodiesterase activity in developing sea urchin eggs. Effects of puromycin, 6-dimethylamino purine and aminophylline.


The pathway from the mediodorsal nucleus of thalamus to the hypothalamus in the cat.


A growth factor for animal cells derived from peptone. II. Effect of the growth factor on cellular metabolism.


Primary afferent hyperpolarization without postsynaptic potentiation.


Caudate intracellular response to thalamic and cortical inputs.


Intracellular responses of caudate neurons to temporally and spatially combined stimuli.


Convergence in the lumbar spinal cord of pathways activated by splanchnic nerve and hind limb cutaneous nerve stimulation.


Membrane junctions in growth and differentiation.


Emergency medical services: new program for old problem.


Anti-insulin eeffect of cortisol in deep hypoglycemia of the rat: sex differences.


Effects of insulin on the lipolytic system of rat fat cells.


Cytochemistry of experimental protein malnutrition in primates: effect on the spinal cord of the squirrel monkey, Saimiri sciureus.


Renin-angiotensin system in chronically hypophysectomized rats.


Metabolic effects of a medium-chain triglyceride emulsion injected intravenously in man.


An investigation of clinical applications of mesomorphic cholesteric liquid crystals in podiatric medicine. A preliminary study.


Angiotensin II like activity in the plasma of normal subjects and patients with renal hypertension.


Serum, spinal fluid and neural total lipids, phospholipids and cholesterol in diabetes mellitus and non-diabetic neuropathy.


Renin-angiotensin system and hypertension.


Motility of spermatozoa and control of bacteria in bovine semen diluents contining penicillin and neomycin or epicillin.


Molecular complexity of Paramecium symbiont lambda deoxyribonucleic acid: evidence for the presence of a multicopy genome.


The typical dream: case studies.

The typical dream: case studies.


Respiratory smooth muscle relaxing effect of commercial steroid preparations.


Histopathological study of a quartz-filled composite dental restorative material.


Hypertension with aldosterone excess.

Hypertension with aldosterone excess.


A vista of research on the mammalian gonadotropins. Carl G. Hartman Lecture.


Evidence of electrotonic transmission in the vestibular nuclei of the rat.


The cyclic AMP system of human brain.

The cyclic AMP system of human brain.


Subcellular distribution of NADH cytochrome c reductase in rat gingiva.


Cortical interneurons during sleep and waking in freely moving primates.


Some evidence for the existence of dopaminergic neurons in the rat cortex.


Effects of serotonin metabolites on sleep-waking activity in cats.


Benzodiazepines and posttetanic potentiation in sympathetic ganglia of the bullfrog.


The modification of the pupillary light reflex by chlorpromazine, diazepam, and pentobarbital.


Responses of single units in the medulla to genital stimulation in estrous and anestrous cats.


The effect of thoracic duct lymph drainage in myasthenia gravis.


Influence of calcium on angiotensin II--antibody reaction.


The interaction of chaotropic anions with acid ferricytochrome c.


Fatty acid inhibition of sulfation factor-stimulated  35 SO 4  incorporation into embryonic chicken cartilage.


Regulation of fatty acid synthesis in isolated hepatocytes prepared from the livers of neonatal chicks.


Relation of glucagon-specific binding sites to glucagon-dependent stimulation of adenylyl cyclase activity in plasma membranes of rat liver.


Yeast inorganic pyrophosphatase. VI. Studies on specificity and mechanism.


Characteristics of the human lymphocyte insulin receptor.


A method for the estimation of some synthetic glucocorticosteroids in rat muscle.


Control of glycogen synthase phosphatase from rat heart. The role of substrate.


The mystery virus. The elusive E-B virus: guilty or not guilty?


The use of rivanol for the removal of nonspecific inhibitors of rubella virus haemagglutinin.


Control of the tonic vibration reflex by the brain stem reticular formation in the cat.


Corticoreticulospinal control of the tonic vibration reflex in the cat.


The role of the contractile vacuole in the osmoregulation of Tetrahymena pyriformis.


m-Chlorobenzhydroxyamic acid--an inhibitor of cyanide-insensitive respiration in Trypanosoma brucei.


Differentiation during encystment and excystment in Oxytricha fallax.


Inhibition of protein synthesis: a mechanism of amebicide action of emetine and other structurally related compounds.


The chick as a laboratory model for the study of estrogen-induced hyperlipidemia.


A sheep in a wolf's coat.

A sheep in a wolf's coat.


Progress in endocrinology and metabolism. Vitamin D: current concepts.


Genetic obesity in rats. II. The effect of food restriction on the metabolism of adipose tissue.


Measurement of plasma renin activity by angiotensin I radioimmunoassay: (I) an assessment of some methodological aspects.


Current status of virological research in human leukemias and sarcomas.


Recruitment, loudness and the stapedial reflex threshold under hypnosis.


The oxygen uptake of alveolar and peritoneal macrophages during phagocytosis of toxic and non-toxic dust particles.


Examination of the liver using radio-active rose bengal. Clinical results of the Department of Radio-Isotopes, H.F. Verwoerd Hospital, Pretoria, 1963-1972.


Cytomegalic inclusion disease. Pediatric grand rounds.


Malignant tumors of infancy and childhood.


The neuron doctrine: a revision of functional concepts.


Enzymatic cyclization of neryl pyrophosphate to  -terpineol by cell-free extracts from peppermint.


Effect of metabolic inhibitors on the agglutination of tumor cells by concanavalin A and Ricinus communis agglutinin.


Antiestrogens: studies using an in vitro estrogen-responsive uterine system.


Cyclic AMP-induced differentiated neuroblastoma cells: changes in total nucleic acid and protein contents.


The interaction of  -cyclodextrin with nucleic acid monomer units.


, -Methylene-adenosine-5'-triphosphate--effects of a competitive inhibitor of adenylate cyclase on cyclic AMP accumulation and lipolysis in isolated fat cells.


The characterization of rat kidney plasma membranes prepared by zonal centrifugation. 1. Plasma membranes were isolated from a 10000g-min pellet prepared from a renal cortical homogenate in 20mm-NaHCO(3) by isopycnic centrifugation in a linear sucrose gradient in an ;A'-type zonal rotor. 2. The preparation was characterized by electron microscopy, and alkaline phosphatase, 5'-nucleotidase, l-leucine beta-naphthylamidase and l-leucine p-nitroanilidase activities were found to be selectively associated with the renal plasma membrane. 3. The preparation had a high degree of purity, as indicated by the presence of low activities of marker enzymes associated with subcellular organelles. A preliminary chemical analysis indicated that the chemical composition resembled that of plasma membranes of other tissues. 4. Plasma membranes were also prepared from tubular fragments and their enzyme contents were found to be similar to those of plasma membranes prepared from cortical homogenates. 5. l-Leucine beta-naphthylamidase, l-leucine p-nitroanilidase and 5'-nucleotidase were not enriched to the same extent as alkaline phosphatase in the preparation of plasma membranes from tubular fragments. A possible explanation for this finding is discussed.


The role of adenosine 3':5'-cyclic monophosphate in the regulation of insulin release. Properties of islet-cell adenosine 3':5'-cyclic monophosphate phosphodiesterase. 1. An assay has been developed with sufficient sensitivity for determination of the adenosine 3':5'-cyclic monophosphate diesterase activity in islets of Langerhans, and has been used to investigate the response of the enzyme to various agents which are known to affect insulin release. 2. The subcellular distribution of the enzyme in islets of Langerhans prepared from guinea-pig pancreas was investigated and over 70% of the activity present in the original homogenate was recovered in the supernatant fraction. 3. Gel filtration of the activity present in the supernatant fraction on Sephadex G-200 gave a single peak of activity with an apparent molecular weight of 200000. The phosphodiesterase activity in the peak fraction showed two apparent K(m) values for adenosine 3':5'-cyclic monophosphate (cyclic AMP) of 3mum and 30mum, suggesting the presence of two activities. The pH optimum of the activity with the low K(m) value was 8.7. 4. Theophylline, caffeine, 3-isobutyl-1-methylxanthine (SC-2964), glibenclamide, tolbutamide, xylitol and leucine were inhibitors of the activity with the low K(m) value; imidazole and arginine stimulated the activity, and glucose and diazoxide were without significant effect. 5. It is suggested that the agents theophylline, caffeine, SC-2964, glibenclamide, tolbutamide, leucine and imidazole may alter the intracellular concentration of cyclic AMP in islets of Langerhans by affecting the cyclic AMP phosphodiesterase activity in islet cells and in this way may affect insulin release.


The effect of two inhalation anesthetics on the order of spin-labeled phospholipid vesicles.


Pressure reversal of inhalation anesthetic-induced disorder in spin-labeled phospholipid vesicles.


Membrane fractions from rat hepatoma. II. Immunochemical characterization of detergent-soluble membrane esterases, glycosidases and leucyl- -naphthylamidase.


Levels of membrane marker enzyme activity in normal and RNA and DNA virus-transformed fibroblasts.


Respiratory viral infections in hospital patients with chronic bronchitis. Observations during periods of exacerbation and quiescence.


Membrane fractions from rat hepatoma. 3. Immunochemical characterization of detergent-soluble membrane phosphatases, electron transport chains and catalase.


Pulmonary blastoma; longterm survival of juvenile patient.


Three simultaneous multiple primary carcinomas in one patient.


Nomenclature explosion.

Nomenclature explosion.


An appraisal of routine methods for the determination of the anodal isoenzymes of lactate dehydrogenase.


The role of cyclic AMP and calcium in cell activation.


The relationship of the level of cyclic amp to differentiation in primary cultures of chick muscle cells.


Effect of 5-bromodeoxyuridine on the structure of differentiating choroid plexus cells in vitro.


Stable alterations in HeLa cell mitochondria following ethidium bromide treatment.


A quantitation of movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts. Experimental methods.


A quantitative difference in the movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts and their polyoma transformants.


Cystosarcoma phyllodes.

Cystosarcoma phyllodes.


Problems peculiar to brain malignancies.


Secondary carcinomatous deposits in the skeletal muscles in association with non-metastatic carcinomatous neuromyopathy.


Absolute configuration of a metabolite in the m-fission pathway of protocatechuate. An aldolase, which is induced in Pseudomonas testosteroni during growth with p-hydroxybenzoate, preferentially attacks the R form of 4-hydroxy-4-methyl-2-oxoglutarate, a metabolite of protocatechuate catabolism.


Membrane lipids of Mycoplasma hominis.

Membrane lipids of Mycoplasma hominis. Essentially all of the lipids of Mycoplasma hominis (200 mug/mg of cell protein) were found to be located in the cell membrane. Over one-half were neutral lipids incorporated from the growth medium and consisting of 43% free cholesterol, 19% esterified cholesterol, 23% triglycerides, 10% free fatty acids, and small amounts of di- and monoglycerides. The polar lipids accounting for about 40% of the total were synthesized by the organisms. Phosphatidylglycerol was the predominant lipid of this fraction. The minor components, tentatively identified as lysophosphatidylglycerol and phosphatidic acid, seem to represent breakdown products of phosphatidylglycerol. No glycolipids were detected. Being unable to synthesize long-chain fatty acids, M. hominis utilized the fatty acids of the growth medium for polar lipid synthesis, preferentially the saturated ones, so that the polar lipids had highly saturated hydrocarbon chains. It is proposed that the large take up of unsaturated neutral lipids and cholesterol from the medium offsets the marked condensing effect of the saturated polar lipids, although electron paramagnetic resonance spectrometry of spin-labeled fatty acids incorporated into the M. hominis membrane indicated that the lipid region is still more rigid than that of the Acholeplasma laidlawii membrane.


Chromatographically fractionated complementary strands of Bacillus subtilis deoxyribonucleic acid: biological properties. Biological, physical, and chromatographic properties of methylated albuminkieselguhr (MAK)-fractionated complementary strands, designated as light (L) and heavy (H), of Bacillus subtilis deoxyribonucleic acid (DNA) are presented. The pattern of transforming activity along the MAK elution profile of alkilidenatured DNA shows that the residually active molecules selectively fractionated ahead of the L strand fraction, whereas the most active self-annealed molecules fractionated preferentially at the trailing end of the H strand fraction. The restoration rate of transforming activity in the late-eluting H molecules was rapid and independent of concentration during the annealing reaction. The data suggest that the self-annealing activity in the H strand is due in part to the formation of intrastrand secondary structures. Hydroxyapatite chromatography of self-annealed L and H strands yielded a major fraction (I) of highly purified strand preparations devoid of transforming activity and hypochromicity, and a minor "nativelike" fraction (II). Sedimentation velocity measurements show that, in addition to the mutual complementary nature of the L and H fractions, they differ in molecular size and possibly configuration.


Control of pantothenate accumulation in Agrobacterium tumefaciens. Two pantothenate-requiring mutants of Agrobacterium tumefaciens have been isolated. One of them (strain WMP-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. Extracts of cells grown on limiting aspartate were found to contain four times more (14)C-pantothenate than those grown at optimal aspartate concentrations. This difference was found in both the perchloric acid-soluble and -insoluble fractions, presumably the coenzyme A pool and acyl carrier protein, respectively. These findings are discussed in terms of membrane integrity and the control of fatty acid biosynthesis.


Chromatographically fractionated complementary strands of Bacillus subtilis deoxyribonucleic acid: transformation of hybrids. The annealing properties as measured by the restoration of transforming activity and hypochromicity of methylated albumin-kieselguhr (MAK)-fractionated complementary strands of Bacillus subtilis deoxyribonucleic acid (DNA) are presented. Temperature-absorbance measurements performed on annealed mixtures of various L and H strand fractions indicated the existence of a complementarity gradient between the two MAK peaks. The markers purA16, leu-8, metB(5), thr-5, and the linked marker hisB(2)-try-2 exhibited different bimodal distributions on MAK columns. The transforming efficiency of heteroduplex mixtures, prepared by cross-annealing resolved complementary strands of wild-type and recipient DNA, was compared. The transforming efficiency of the wild-type L and H strands was equal in one preparation and unequal in a second preparation. It was found that in the second strand preparation the heteroduplex DNA containing the H strand from wild type was more efficient for all of the markers tested. The variations in transforming efficiencies of the complementary strands in heteroduplex molecules reported here and by others are due in part to strands of unequal length and probably to the self-annealing property of the H strands. At present, no conclusion could be made regarding the existence of strand selection bias during integration of donor DNA in competent B. subtilis cells.


Role of adenosine 3',5'-cyclic monophosphate and its specific binding protein in the regulation of D-serine deaminase synthesis. Adenosine 3',5'-cyclic monophosphate (cyAMP) and the cyAMP-binding protein are necessary for efficient induction of d-serine deaminase (Dsdase) synthesis in dsdC(+)dsdO(+) and dsdC dsdO(+) strains of Escherichia coli K-12, and for constitutive synthesis in dsdC dsdO(+) strains. Neither is required in dsdO strains, confirming previous indications that in dsdO mutants Dsdase synthesis is not subject to catabolite repression control. Since efficient Dsdase synthesis in dsdC(+) and dsdC strains that are dsdO(+) requires the cyAMP-binding protein, which acts at the level of transcription in other systems, it is concluded that catabolite repression acts at the level of transcription of the Dsdase structural gene. Since catabolite repression is reversed by the inducer, d-serine in dsdC dsdO(+) strains, it is concluded that induction also acts at the level of transcription in this system. The dsdC strains were found to be much more sensitive to induction by d-serine than are dsdC(+) strains under conditions of catabolite repression, whether the repression was caused by glucose or by loss of ability to form cyAMP or cyAMP-binding protein. This suggests that a d-serine-dsdC complex may be able to replace partially the cyAMP:cyAMP-binding protein action in initiation of Dsdase messenger ribonucleic acid synthesis-a positive control effect.


Isolation and characterization of mutations affecting the transport of hexose phosphates in Escherichia coli. Mutants of Escherichia coli defective in the hexose phosphate transport system were isolated. Negative selection by penicillin treatment or positive selection with phosphonomycin was employed. These mutants grew normally on all carbon sources other than hexose phosphates. The map location of the mutations in 18 independently isolated mutant strains was investigated by transduction crosses. All of the mutations were found to lie in the same region of the chromosome, in the region represented by min 72 on the Taylor map. The order of the genes in this region was found to be mtl-cysE-pyrE-uhp-bgl-ilv. Revertants of some of the mutants exhibited altered regulatory control of this transport system.


Metabolism of D-fructose by Arthrobacter pyridinolis. Previous studies showed that Arthrobacter pyridinolis can transport and utilize d-glucose only after prior growth on certain Krebs cycle intermediates. In contrast, we found that d-fructose was taken up and metabolized by A. pyridinolis without special prior conditions of growth. d-Fructose was first converted to d-fructose-1-phosphate by a phosphoenolpyruvate (PEP):D-fructose phosphotransferase. This activity required both supernatant and pellet fractions from d-fructose-grown cells centrifuged at 150,000 x g. The d-fructose-1-phosphate formed was converted to d-fructose-1, 6-diphosphate. Mutants deficient in PEP:d-fructose phosphotransferase and d-fructose-1-phosphate kinase, or d-fructose-1, 6-diphosphatase (FDPase) were unable to grow on d-fructose but retained the normal ability to use d-glucose. Mutants forming reduced amounts of FDPase were completely unable to grow on d-fructose but were still capable of limited growth on Krebs cycle intermediates. A requirement for higher levels of FDPase for growth on d-fructose than for growth on Krebs cycle intermediates was also indicated by the higher specific activities of FDPase in d-fructose-grown cells than in cells grown on l-malate or amino acids.


Time of enterotoxin formation and release during sporulation of Clostridium perfringens type A. Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase in biologically active toxin coincided with the increase in enterotoxin antigen. Enterotoxin was released from the sporangium with its lysis, concomitantly with the mature spore release.


A comparison of two methods of prognostic typing in breast cancer. The value of two different systems for the prognostic assessment of breast cancer has been tested by comparing a group of 74 long-term survivors with a group of 54 short-term survivors. The system devised by Bloom and Richardson (1957) gave a good general correlation with prognosis. Appearances described by Harveit (1971) showed some correlation with prognosis but were rarely seen in our cases. The degree of lympho-plasmacytic infiltrate did not show any correlation with prognosis.


Effects of reserpine on sleep and brain biogenic amine levels in the cat.


Juvenile nasopharyngeal haemangiofibroma.


Herpes simplex and zoster.

Herpes simplex and zoster.


Actinomycin D for Wilms's tumour.

Actinomycin D for Wilms's tumour.


Oral contraceptives.

Oral contraceptives.


Physiological and genetic studies of polyoma virus.


Pseudo-cowpox (paravaccinia) in dairy cows.


Bronchography in diseases of the adult chest.


Radiographic aspects of pulmonary infection in patients with altered immunity.


Maternal-fetal endocrine interrelations: influence of maternal adrenocorticosteroids on fetal ACTH secretion.


Angiotensin II, diuretics, and thoracic duct lymph flow in the dog.


Cheap labor from poor nations.

Cheap labor from poor nations.


Stimulation of aldosterone secretion by hemorrhage in dogs after nephrectomy and decapitation.


Roentgen evaluation of Wilms' tumor.

Roentgen evaluation of Wilms' tumor.


Synthesis of cyclic AMP and phosphodiesterase in various species of cellular slime molds and its bearing on chemotaxis and differentiation.


Metabolism of ethanol and fructose in the isolated perfused pig liver.


A sequence of 50 nucleotides from coliphage R17 RNA.


Chemodectoma with metastasis to bone in a dog.


Metabolism of phenol and cresols by mutants of Pseudomonas putida. Mutant strains of Pseudomonas putida strain U have been obtained which are deficient in enzymes of the degradative pathways of phenol and cresols. Mutant strains deficient in catechol 2, 3-oxygenase accumulated the appropriate catechol derivative from cresols. A mutant strain which would not grow on either phenol or a cresol was shown to be deficient in both 2-hydroxymuconic semialdehyde hydrolase and a nicotinamide adenine dinucleotide, oxidized form, (NAD(+))-dependent aldehyde dehydrogenase. When this strain was grown in the presence of phenol or a cresol, the appropriate product of meta fission of these compounds accumulated in the growth medium. A partial revertant of this mutant strain, which was able to grow on ortho- and meta-cresol but not para-cresol, was shown to have regained only the hydrolase activity. This strain was used to show that the products of meta ring fission of the cresols and phenol are metabolized as follows: (i) ortho- and meta-cresol exclusively by a hydrolase; (ii) para-cresol exclusively by a NAD(+)-dependent aldehyde dehydrogenase; (iii) phenol by both a NAD(+)-dependent dehydrogenase and a hydrolase in the approximate ratio of 5 to 1. This conclusion is supported by the substrate specificity and enzymatic activity of the hydrolase and NAD(+)-dependent aldehyde dehydrogenase enzymes of the wild-type strain. The results are discussed in terms of the physiological significance of the pathway. Properties of some of the mutant strains isolated are discussed.


Salmonella typhimurium mutants lacking ribonuclease I: effect on the polarity of histidine mutants. Mutants of Salmonella typhimurium containing 1 to 2% of wild-type ribonuclease I activity were isolated. The rns mutation had no effect on the polarity of mutations in the S. typhimurium histidine operon. Even in the presence of an rns mutation, it was not possible to obtain strong suppressors of the polarity of two polar mutations in the his operon.


Purification and identification of the fruiting-inducing substances in Coprinus macrorhizus. Substances which are effective in inducing fruiting bodies in monokaryotic mycelia of the fis(+) strain of Coprinus macrorhizus were purified and characterized. The active components of fruiting-inducing substances were identified as adenosine-3'-monophosphate, adenosine 3',5'-cyclic monophosphate (cyclic AMP), and a protein which is bound with the cyclic AMP. Cyclic AMP was synthesized from adenine within mycelia of the mutant strains which form monokaryotic fruiting bodies without the addition of fruiting-inducing substances, but not in those of the strains which do not form monokaryotic fruiting bodies. The proteins which bind with cyclic AMP were detected in crude extracts of mycelia of those strains which form monokaryotic fruiting bodies and of the dikaryon, but not in those of the strains which do not form monokaryotic fruiting bodies.


Metabolism of adenosine 3',5'-cyclic monophosphate and induction of fruiting bodies in Coprinus macrorhizus. The adenyl cyclase and phosphodiesterase metabolizing adenosine 3',5'-cyclic monophosphate (cyclic AMP) were detected in mycelia of strains of Coprinus macrorhizus which form fruiting bodies, but not in those of strains which do not form fruiting bodies. The adenyl cyclase synthesized cyclic AMP from adenosine triphosphate. The phosphodiesterase degr[UNK]ded cyclic AMP to adenosine-5'-monophosphate and was inhibited by adenosine-3'-monophosphate, theophylline, and caffeine. The strains which form fruiting bodies incorporated and metabolized cyclic AMP, but strains which do not form fruiting bodies did not. The possible participation of cyclic AMP in the induction of fruiting bodies is discussed.


Sequential metabolic events during encystment of Azobacter vinelandii. Cells of Azotobacter vinelandii are specifically induced to encyst by beta-hydroxybutyrate (BHB). The process of differentiation, which occurs over a period of 36 h, was characterized by an ordered sequence of biochemical events. Upon initiation of encystment, nitrogen fixation and glucose-6-phosphate dehydrogenase activities decreased immediately to very low levels. This was followed by an increase in the specific activities of BHB dehydrogenase, isocitrate dehydrogenase, isocitrate lyase, and malate synthase first at 3 h and then again at 21 h. The peak activity of fructose 1,6-diphosphate aldolase occurred at 6 h, and the enzyme activity then decreased gradually. Fructose 1,6-diphosphatase had peak activities at 9 and 27 h. Deoxyribonucleic acid synthesis ceased just prior to the final cell division at 4 to 6 h, but ribonucleic acid synthesis continued until the 12th h. From labeling studies and the appearance of new enzyme activities, it appeared that protein synthesis continued throughout encystment.


Proton magnetic resonance spectroscopy of Klebsiella capsular polysaccharides. The presence of acetate and pyruvate groups in Klebsiella capsular polysaccharides may be demonstrated and estimated quantitatively by running the proton magnetic resonance spectrum of the polysaccharide (as sodium salt) in deuterium oxide at 95 C. Such spectra also permit an assessment to be made of the number of alpha- and beta-linkages in the repeat unit of the polysaccharide structure.


Regulation of manganese accumulation and exchange in Bacillus subtilis W23. An overnight culture of Bacillus subtilis W23 in low-manganese tryptone broth is unable to sporulate and becomes hyperactive with regard to the manganese active transport system during stationary phase. When manganese is added to cells in spent or fresh medium, the cells immediately accumulate a high proportion of the manganese available in the medium. When the hyperactive cells are diluted into broth containing 10 muM Mn(2+), high intracellular manganese levels are reached, and inhibition of ribonucleic acid and protein synthesis occurs. This inhibition is relieved when the intracellular manganese concentration declines to the nontoxic levels characteristic of cells growing in 10 muM Mn(2+). The release of the accumulated manganese is achieved by a reduction in the uptake rate for manganese while the efflux rate remains essentially constant. Inhibitors of ribonucleic acid and protein synthesis prevent the reduction of the high rate of manganese uptake and, therefore, high net concentrations of manganese are maintained in the presence of these inhibitors. The hyperactive manganese uptake system is temperature dependent and inhibited by cyanide and m-chlorophenyl carbonylcyanide hydrazone.


Fine structure of Cryptococcus neoformans grown in vitro as observed by freeze-etching. Cryptococcus neoformans grown on culture media was observed by the freeze-etching technique. In the capsule, short fibrils were seen when freezeetched. This organism was unique in the appearance of the cell wall, which showed two strata. The outer one was dense with particles of about 20 nm in diameter, whereas the inner one was sparse in particles. The appearance of the cell membrane of this organism differed distinctly depending on the culture media. When grown on glycerol medium, the cell membrane possessed, as do other yeasts, clear but somewhat longer and curved invaginations. The membrane of cells grown on nonglycerol medium exhibited, however, only a few invaginations of irregular shape. Instead, characteristically of this organism, the cell membrane showed round depressions of 40 to 200 nm in diameter which were the surface view of the paramural bodies. In cross-fractured cells, both types of paramural bodies were found. Some of them contained a single vesicle of about 50 nm in diameter. These seem to play a role in secreting the cytoplasmic vesicles. Data suggesting the existence of multivesicular bodies in the cytoplasm and multivesicular lomasomes were also obtained. Some of the baglike paramural bodies showed multilayered membrane. These are thought to be plasmalemmasomes. This organism was similar to other yeasts reported in other respects.


Requirement of adenosine-3',5'-cyclic monophosphate for L-arabinose isomerase synthesis in Escherichia coli. Adenosine-3', 5'-cyclic monophosphate (cyclic AMP) is essential for the synthesis of l-arabinose isomerase in Escherichia coli. Cyclic AMP appears to be required for the transcription of deoxyribonucleic acid into messenger ribonucleic acid (RNA), since the enzyme synthesis is not observed in induced cells to which cyclic AMP is added after messenger RNA synthesis is arrested by rifampin or after inducer removal.


Cyclic-nucleotide phosphodiesterase: an early defect in inherited retinal degeneration of C3H mice. Cyclic nucleotides have been implicated in the differentiation and function of the vertebrate retina. In the normal retina of DBA mice, the specific activity of cyclic-nucleotide phosphodiesterase (PDE), with cyclic-AMP as the substrate (cAMP-PDE), increases eightfold between the 6th and 20th postnatal day. Kinetic analysis of retinae from newborn mice reveals a PDE with a single Michaelis constant (K(m)) value for cyclic-AMP (low K(m)-PDE). After the 6th postnatal day, a second PDE with a high K(m) for cyclic-AMP (high K(m)-PDE) can be demonstrated. The appearance and increasing activity of the high K(m)-PDE coincides with the differentiation and growth of photoreceptor outer segments. Additionally, the high K(m)-PDE is shown by microchemical techniques to be concentrated in the photoreceptor cell layer and the low K(m)-PDE within the inner layers of the normal retina. In C3H mice afflicted with an inherited degeneration of the photoreceptor layer, the postnatal increase in the specific activity of cAMP-PDE is substantially lower than in the normal retina. The postnatal increase in the specific activity of cAMP-PDE in two regions of the brain of C3H mice is the same as in the normal strain. A deficiency in high K(m)-PDE activity in the C3H retina is evident on the 7th postnatal day, when the activity of low K(m)-PDE, photoreceptor morphology, and rhodopsin content of these retina are essentially normal. In the adult C3H retina, the PDE activity with cyclic-GMP and cyclic-UMP as substrates is significantly below that of the normal retina. These data indicate that an alteration in cyclic-AMP metabolism occurs before photoreceptor cell degeneration in the retinae of C3H mice.


Cell membrane resorption in the rat exocrine pancreas cell after in vivo stimulation of the secretion, as studied by in vitro incubation with extracellular space markers. Pancreatic secretion in the rat was stimulated in vivo by pilocarpine injection causing 90% of the storage granules to be discharged within 2 h. Incubation in vitro with [(14)C]sorbitol indicated that maximal ingestion of this extracellular space marker occurred 3 h after secretogogue injection. Morphological cell membrane measurements on cells with stimulated secretion revealed a simultaneous decrease in amount of membrane bordering the microvilli at the cell apex, lamellar processes, and infoldings present at the latero-basal face of these cells. In 3-h stimulated cells, having the average zymogen granule content characteristic for that phase of secretion, ferritin treatment in vitro showed that the infoldings and related fragmentation vesicles had ingested ferritin and could consequently be considered as being transport vehicles for redundant cell membrane. During stimulated secretion numerous vesicles and vacuoles appeared in the apical cytoplasm. Part of these structures were postulated to be related to the Golgi complex and were discussed in relation to secretory protein transport. Another part of these structures was assumed to have an endocytotic nature, although they never contained ferritin.


Growth of normal and transformed rat embryo fibroblasts: effects of glycolipids from Salmonella minnesota R mutants. Addition of glycolipids obtained from Salmonella minnesota R mutants to normal, spontaneously transformed, and SV40-transformed rat embryo fibroblasts in culture results in an inhibition of growth of transformed cells but not of normal cells. In the presence of the glycolipid with the smallest carbohydrate chain length, spontaneously transformed cells stop growing when they reach confluency. Inhibition of growth of transformed cells is inversely related to the chain length of the core sugars. Glycolipid mR595 is shown to bind with the cell membrane of transformed cells and elicits an augmentation in the intracellular level of cyclic AMP. Normal cells bind relatively less glycolipid mR595 and show a lower percent of increase in cyclic AMP due to glycolipid mR595 than do transformed cells.


Membrane fusion during mast cell secretion.


Secretion of collagen by corneal epithelium. I. Morphology of the collagenous products produced by isolated epithelia grown on frozen-killed lens. Corneal epithelium from 5-7-day old chick embryos was isolated with EDTA and grown in culture on frozen-killed lens as a substratum. Autoradiographs showed that in the presence of [(3)H]proline, the corneal epithelium synthesized and secreted onto the lens substratum, radioactive materials resistant to extraction by sodium hydroxide. The radioactive label was associated with newly formed striated collagen fibrils, large "sheets" of collagen, and basal lamina. The repeat period and interband pattern of the abundant new collagen sheets and fibrils was typical of "native" or so-called "mesenchymal" collagen. Collagen-like materials were observed in secretory (Golgi) vacuoles within the corneal cells and collagen fibrils within the intercellular canals (lateral interfaces) of the epithelium, as well as at the base of the cells. Both the granular endoplasmic reticulum and Golgi complexes were highly developed in the corneal epithelium. In the discussion, the role of cytoplasmic organelles in collagen secretion, the origin and structure of the basal lamina, and variations in collagen polymerization patterns in vitro are reviewed and evaluated. The morphogenetic significance of the synthesis and secretion of collagen by embryonic epithelium is appraised and the production of true native-striated collagen by epithelium is stressed.


Dimethyl sulfoxide-induced reversion of several features of polyoma transformed baby hamster kidney cells (BHK-21). Alterations in growth and morphology. Cells of a polyoma virus transformed clonal line (Cl-I) of baby hamster kidney fibroblasts (BHK-21) were grown in medium containing 2 percent dimethylsulfoxide (DMSO). Unlike the untransformed BHK-21 cells, Cl-I cells adapted to replication in the presence of DMSO, and they exhibited a rapidly reversible phenotypic reversion of a number of properties characteristic of the transformed state. Restoration of density dependent growth inhibition with accumulation of cells in the G(1) phase of the cell cycle occurred and was associated with restoration of contact dependent behavior and with reversion of histological and ultrastructural features towards those which characterize untransformed cells. Concomitantly, Cl-I cells grown in 2 percent DMSO lost the ability to form colonies in semisolid medium. The data presented suggest that DMSO alters the expression of cellular functions which were altered as a result of viral transformation and which may be involved in cell tumorigenicity.


Lung uptake of  99m Tc-sulfur colloid.

Lung uptake of  99m Tc-sulfur colloid.


Interpretation of the  67 Ga photoscan.

Interpretation of the  67 Ga photoscan.


Periodic variation in A-V conduction time: the 'supernormal' phase of A-V conduction. A study in differential dual A-V pathway conductivity and refractoriness.


Axonal agranular reticulum and synaptic vesicles in cultured embryonic chick sympathetic neurons. Cultured chick embryonic sympathetic neurons contain an extensive axonal network of sacs and tubules of agranular reticulum. The reticulum is also seen branching into networks in axon terminals and varicosities. The axonal reticulum and perikaryal endoplasmic reticulum resemble one another in their content of cytochemically demonstrable enzyme activities (G6Pase and IDPase) and in their characteristic membrane thicknesses (narrower than plasma membrane or some Golgi membranes). From the reticulum, both along the axon and at terminals, there appear to form dense-cored vesicles ranging in size from 400 to 1,000 A in diameter. These vesicles behave pharmacologically and cytochemically like the classes of large and small catecholamine storage vesicles found in several adrenergic systems; for example, they can accumulate exogenous 5-hydroxydopamine. In addition, dense-cored vesicles at the larger (1,000 A) end of the size spectrum appear to arise within perikaryal membrane systems associated with the Golgi apparatus; this is true also of very large (800-3,500 A) dense-cored vesicles found in some perikarya.


Apomorphine antagonism of the elevation of homovanillic acid induced by antipsychotic drugs.


Effects of angiotensin II on cyclic 3'5'AMP in rat uterus.


Effect of cholecystokinin on the level of cyclic AMP and on mechanical activity in the isolated sphincter of Oddi.


Spontaneous transmitter release and ACh sensitivity during glutaraldehyde fixation of rat diaphragm.


A functional connection between postganglionic neurons in a bullfrog sympathetic ganglion.


Adenyl cyclase-phosphodiesterase system in arterial smooth muscle.


Evidence for sodium dependent outward transport of the  3 H-norepinephrine mobilized by calcium at the adrenergic synapse. Inhibition of transport by desipramine.


The mechanism of the drinking response to some hypotensive drugs: activation of the renin-angiotensin system by direct or reflex-mediated stimulation of  -receptors.


Two morphologically distinct blood-brain barriers preventing entry of cytochrome c into cerebrospinal fluid. After intravenous injection, cytochrome c does not enter the cerebrospinal fluid. In most areas of the brain, the marker is prevented from leaving cerebral vessels by the capillary endothelium. In the choroid plexus, the marker passes freely out of capillaries into the extracellular space. However, it does not traverse tight junctions between epithelial cells and is rapidly incorporated into mnembrane-bound vesicles within the cell cytoplasm. Thereafter, cytochrome c is apparently removed by lysosomal degradation. These data suggest that there are at least two morphologically distinct blood-brain barriers to cytochrome c and that pinocytosis may be a mechanism for intracellular degradation rather than transcellular transport.


Effects of colchicine and cytochalasin B on the release of  3 H-norepinephrine from guinea-pig atria evoked by high potassium, nicotine and tyramine.


Immunologic manipulation of metastases due to herpesvirus transformed cells. Herpes simplex virus type 1 (HSV-1), type 2 (HSV-2), and simian virus 40 (SV40) fail to induce immunity in weanling Syrian hamsters to transplant of hamster cells transformed by HSV-2. However, the development of metastatic tumors is markedly enhanced by prior immunization with HSV-1. Immunization with SV40, ultraviolet-irradiated tumor cells, or ultraviolet-irradiated normal hamster embryo cells inhibits the development of metastases. The HSV-hamster system appears a good one for the study of development, prevention, and control of metastases by mammalian cells transformed by a common human virus.


Development of tone-burst responses along the auditory pathway in the cat.


Neonatal hypoglycaemia due to an islet-cell adenoma.


Immunosuppression during pregnancy: transmission of azathioprine and its metabolites from the mother to the fetus.


Passage of polymyxins from serum into milk in ewes.


Failure of either corticosteroids or ACTH to increase the leukocyte concentration in milk.


Clinical course and histopathologic features of pseudorabies virus-induced keratoconjunctivitis in pigs.


Oncogenic activity of feline fibrosarcoma virus in newborn pigs.


Generation of phenoxy radicals by methemoglobin-hydrogen peroxide studies by electron paramagnetic resonance.


Cyclic AMP phosphodiesterase activity at the external surface of intact skeletal muscles and stimulation of the enzyme by insulin.


The effects of local anesthetics on lipid multilayers. A spin probe study.


The hydrolysis of triphosphoinositide by a phosphodiesterase in rat kidney cortex.


A membrane-bound alkaline inorganic pyrophosphatase in isolated spinach chloroplasts.


Collagen proline hydroxylase activity in the uterus of the rat during rapid collagen synthesis in vivo.


Deuterium isotope effects in the oxidation of alcohols in vitro and in vivo.


Estimation of plasma renin activity by radioimmunoassay of angiotensin I.


Diphosphopyridine nucleotide-linked D-lactate dehydrogenases from the horseshoe crab, Limulus polyphemus and the seaworm, Nereis virens. I. Physical and chemical properties.


Protein synthesis in chicken muscular dystrophy.


Interaction of pyruvate with pyruvate carboxylase and pyruvate kinase as studied by paramagnetic effects on  13 C relaxation rates.


Diphosphopyridine nucleotide-linked D-lactate dehydrogenases from the horseshoe crab, Limulus polyphemus, and the seaworm, Nereis virens. II. Catalytic properties.


Measurement of the water exchange rate of bound water in the manganese(II)-adenosine triphosphate complex by oxygen-17 nuclear magnetic resonance.


A stimulation by phosphate of malate transport and oxidation in rat adrenal mitochondria.


The effect of maternal hypoxia on fetal pituitary hormone release in the sheep.


Biochemical properties of human fetal mitochondria.


Usefulness of solid medium in Myjak's application (3) for current laboratory diagnostics of amoebiasis.


Effects of morphine on the Golgi apparatus and mitochondria of mammalian neurones.


Chromosome and chromatid exchanges in Chinese hamster cells.


Bronchogenic carcinoma: the true story.

Bronchogenic carcinoma: the true story.


Nobel Prize winner talks about noradrenaline, nerves and aging.


Reticulum cell sarcoma and malignant histiocytoma of the soft tissues.


Sequential studies on spontaneous mammary and hepatocarcinogenesis in reciprocal crosses of ICRC and C3H (Jax) strains.


Mapping of sites on the surface membrane of mammalian cells. II. Relationship of sites for concanavalin A and an ornithine, leucine copolymer.


Neuropathy of metachromatic leucodystrophy. We describe two patients with metachromatic leucodystrophy who presented with peripheral neuropathy and had no evidence of central nervous system involvement at the time of presentation. Such an occurrence is not well recognized and the diagnosis is usually missed until the other features of the disease manifest themselves. Early recognition of this disease may become of practical importance from the point of view of therapy, judging from recent developments, and is also important from the point of view of genetic counselling. We suggest that cases of peripheral neuropathy in infancy and childhood, should be subjected to peripheral nerve biopsy when the aetiology is not obvious.


Differential sensitivity of motor and sensory fibres in human ulnar nerve. The human ulnar nerve has been stimulated with square-wave pulses of various fixed durations. Measurements were made of the growth of hand myograms compared with elbow neurograms and of hand myograms compared with finger neurograms, for fixed durations of pulses and increasing strength. The effect of blocking sensory action potentials with stimuli of various durations has been investigated, as well as the blocking of action potentials in motor nerve fibres by voluntary activity. It is concluded that pulses of long duration (1 msec or more) selectively stimulate sensory fibres at threshold, whereas short duration pulses (less than 200 μsec) selectively stimulate motor fibres.


Effect of procaine infiltration into biceps brachii muscle in man on supraspinal control of reflex transmission. Sensitivity of the tonic stretch reflex (TSR) of biceps brachii in normal man was measured at different levels of voluntary contraction before and after gamma efferent nerve fibres were selectively blocked by infiltration of dilute procaine. The procaine infiltration reduced TSR sensitivity to one half of its control value. It had no effect, however, on the slope of the relation between TSR gain and level of voluntary contraction. It was concluded that the increase in TSR sensitivity associated with voluntary contraction was not mediated by the gamma efferent fibres and control of reflex transmission is therefore not the main function of the fusimotor system.


Metabolic neuropathy.

Metabolic neuropathy.


Cytotoxic effect of viruses on Harding-Passey melanoma in tissue culture.


Calcium, cyclic adenosine 3',5'-monophosphate, and the control of cell proliferation: a review.


Induction of virus synthesis in polyoma-transformed BHK-21 cells. BHK-21 cells were transformed with polyoma virus mutants Ts-a and Ts-25 by using a temperature shift from 31 to 39 C at 5 days after infection so that rescuable transformants could be isolated. Clones which yielded virus after fusion with mouse cells were scored and maintained at 39 C in the presence of antipolyoma virus antiserum. Generally, no infectious viral deoxyribonucleic acid (DNA) could be found in Hirt supernatant fractions of these lines when maintained at 39 C, but DNA-DNA reannealing measurements detected two to six viral genomes per diploid cell genome in the nuclear DNA. Fusion with permissive cells was not necessary to induce the synthesis of infectious virus; cell lines shifted to 31 C produce the equivalent of 100 viral genomes per cell after 5 days. In some cell lines up to 1% of the cells formed infectious centers upon a shift to 31 C, and 100% of the subclones of a line were inducible. Growth at 31 C selected for a noninducible population which was still transformed.


The influence of rare earths on hepatic gluconeogenesis.


Oxaluria.

Oxaluria.


Effect of dibutyryl cyclic-AMP on levels of dopamine- -hydroxylase in isolated superior cervical ganglia.


Actions of various cyclic nucleotides, nucleosides and purine bases on the synthesis of corticosterone in vitro.


Erythropoiesis in the yolk sac of the early chick embryo: an electron microscope and microspectrophotometric study.


Light-induced changes in the ultrastructure of a plasmodial myxomycete.


Freeze-fractured lamellar body membranes of the rat lung great alveolar cell.


Ultrastructural changes in the nuclear envelope of larval fat body cells of Simulium vittatum (Diptera) induced by microsporidian infection of Thelohania bracteata.


Histology and ultrastructure of the tube foot epithelium in the phanerozonian starfish, Astropecten.


Cytodifferentiation of maturing pine tracheids: the final stage.


The ultrastructure and function of pericardial cells and other nephrocytes in an insect: Calliphora erythrocephala.


Fibrous xanthomas and xanthosarcomas of the meninges and the brain.


Hemorrhage from cecal ulcers of cytomegalovirus infection: report of a case.


Oncogenic viruses.

Oncogenic viruses.


Susceptibility of chick brain L-glutamic acid decarboxylase and other neurotransmitter enzymes to hyperbaric oxygen in vitro.


Testosterone-stimulation of adenyl cyclase and cyclic 3',5'-adnosine monophosphate- 3 H formation in rat seminal vesicles.


Effects of clofibrate, in vitro, on mitochondrial respiration and oxidative phosphorylation.


Haem protein P-450 from the adrenal cortex: interaction with steroids and the hydroxylation reaction.


Structure and reactions of oxygenase active centers: cytochrome P-450 and iron sulfur proteins.


Cholesterol hydroxylation in the adrenal cortex and liver.


A study of transfer RNA methylation.

A study of transfer RNA methylation.


Hydroxylamine-dependent reactions catalyzed by a lysyl-tRNA synthetase of Escherichia coli B.


Kinetics of synthesis of cytochromes   and   3  during respiratory adaptation of yeast.


Purification of superoxide dismutase from fungi and characterization of the reaction of the enzyme with catechols by electron spin resonance spectroscopy.


Cyclic AMP-induced differentiated mouse neuroblastoma cells lose tumourgenic characteristics.


Ultrastructure of lipid bodies and lysosomes in the skin in Fabry's disease (angiokeratoma corporis diffusum).


Alkaline and acid phosphatase in the intestines of rats infected with a virulent strain of Entamoeba histolytica and treated with emetine and Flagyl.


Microspectrophotometric analysis of malarial pigment.


Schistosoma mansoni: histochemical analysis of the postacetabular gland secretion of cercariae.


Transient increase in capillary permeability induced by Clostridium perfringens type A enterotoxin. A transient increase in capillary permeability was induced in the skin of guinea pigs by Clostridium perfringens type A enterotoxin. Maximum intensity and maximum diameter of the area of increased capillary permeability were obtained with injection of enterotoxin 10 to 20 min before dye injection.


Foot-and-mouth disease virus: selection by homogenized calf kidney adsorption and cell culture passage. Foot-and-mouth disease virus was passaged 20 times by alternately adsorbing the virus with homogenized calf kidney and propagating the adsorption-resistant fraction of virus in cell cultures. Passage of the virus was accompanied by a marked decrease in pathogenicity for 35-day-old mice and a reduction in its ability to adsorb to homogenized kidney from such mice. Adsorption by homogenized calf kidney and infant mouse kidney also decreased with passage, but pathogenicity for cattle and infant mice remained high. The passaged virus did not seem to change antigenically. When the 20th-passage virus was serially passaged 10 times in cell cultures, no reversion in pathogenicity for 35-day-old mice was observed.


Saccadic inhibition induced by brain-stem stimulation in the alert monkey.


Conformational changes in membranous preparations of cytochrome oxidase.


Isolation and characterization of Mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis. Fourteen conjunctival strains of mycoplasma were isolated from infected sheep and goats in two separate naturally occurring outbreaks of keratoconjunctivitis (pink-eye). The biological and serological properties and cell protein patterns of these strains are presented in this report. These strains were related to each other and were unrelated to 40 recognized Mycoplasma and Acholeplasma species and serotypes. We are proposing that this group of mycoplasmas be named M. conjunctivae. The role of M. conjunctivae in the etiology of keratoconjunctivitis in sheep and goats remains to be determined.


Proteins of cytochrome oxidase in fractions prepared with deoxycholate from bovine heart submitochondrial particles.


Variability of the K+-Na+ discrimination of beauvericin in mitochondrial membranes.


In vitro transformation assay of major fractions of cigarette smoke condensate (CSC) in mammalian cell lines.


Changes in rat liver plasma membrane phospholipids during aging.


The effect of chloroquine on herpesvirus infection in vitro and in vivo.


The nature of collagen in the carrageenin granuloma.


Effect of cyclophosphamide on the genetic resistance of C 3 H mice to mouse hepatitis virus.


The effects of prostaglandin E 1  on erythropoietin production.


Effect of angiotensin II on uptake and release of dl- 3 H-metaraminol.


Regulation of the in vitro synthesis of angiotensin I.


Cation activated hydrolysis of ATP by the soluble fraction of Bacillus brevis.


Reaction of endogenous renin with exogenous renin substrate within the isolate perfused rat kidney.


Possible mechanisms of impaired interferon production in stressed mice.


An in vivo corticotropin-releasing factor (CRF) assay based on plasma levels of radioimmunoassayable ACTH.


Testosterone-induced depression of adrenergic activity in the perfused canin hindlimb.


Extracellular localization of lysozyme in rachitic rat cartilage.


Pyruvate dehydrogenase, the citrate condensing enzyme and the utilization of  14 C-labeled lactate, pyruvate and alanine by slices of lactating mammary gland and adenocarcinoma of mouse mammary gland.


Infection-mediated resistance to cell fusion by inactive Sendai virus.


Sensitivity of human conjunctival tissue cultures to adenovirus 8, herpes simplex and vaccinia viruses. Induction of resistance by an interferon inducer.


Ultrastructural modifications of frog urinary bladder epithelium under the influence of hypertonic media.


Angiography in the diagnosis and management of extrapancreatic islet-cell tumors.


Histologic patterns of secondary syphilis.


Recurrent skin cancer.

Recurrent skin cancer.


Lateral geniculate spikes and eye movements in sleeping and awake cats following uni- and bilateral labyrinthectomy and cerebellar lesion.


Studies on kinin-like substances in brain.


Inhibitory effects of chlorpromazine and diethylaminoethyl diphenylvalerate (SKF 525-A) on alanine incorporation into protein and  -aminoisobutyric acid uptake in rat hepatoma cells in culture.


Effects of sodium salicylate and acetylsalicylic acid on the lipolytic system of fat cells.


Antiparasitic nitroimidazoles. IV. Serum protein binding and mode of action of 2-(4'-carboxystyryl)-5-nitro-1-vinylimidazole.


Effect of rifampicin on mitochondrial protein synthesis in rat liver.


Epstein-Barr virus antibody levels in systemic lupus erythematosus.


Effect of   9 - and   8 -tetrahydrocannabinol on the peripheral autonomic nervous system in vitro.


The inhibition of sympathetic preganglionic neurons by somatic afferents.


Vitamin A status and hepatic drug metabolism in the rat.


Hyperlipoproteinemia. Prevalence in a free-living population in Albany, New York.


Properties of the elongation factors from Escherichia coli. Exchange of elongation factor G during elongation of polypeptide chain.


Purification of a phosphodiesterase from Bothrops atrox venom by affinity chromatography.


Cytochrome c: a thermodynamic study of relationships among oxidation state, ion-binding and structural parameters. 2. Ion-binding linked to oxidation state.


Cytochrome c: a thermodynamic study of the relationships among oxidation state, ion-binding and structural parameters. 1. The effects of temperature, pH and electrostatic media on the standard redox potential of cytochrome c.


Metabolic regulation of steroidogenesis in adrenocortical carcinoma cells of rat. Effect of adrenocorticotropin and adenosine cyclic 3':5'-monophosphate on corticosteroidogenesis.


Pleuro-pulmonary complications of amoebiasis.


Hepato-pulmonary amoebiasis.

Hepato-pulmonary amoebiasis.


The effect of topical corticosteroids on the susceptibility of immune animals to reinoculation with Herpes simplex.


A modified method for the isolation of melanosomes from B16 melanoma.


'Collagenase-like peptidase in human, rat and bull spermatozoa.


Relationship between ligand binding and conformational change of macromolecules in the two-state allosteric model.


A general-purpose electronic model for arbitrary configurations of neurons.


Theoretical implications of the size principle of motoneurone recruitment.


Association of two contiguous urological tumors with adult Wilms tumor.


Intraductal carcinoma of the prostate gland.


Wilms tumor: a clinical pathological correlation.


Subcellular muscle studies in the prune belly syndrome.


The neuroleptics. 2. Neurophysiology and neuropharmacology.


Histological and molecular biochemistry.


SV40 virus-specific RNA synthesis in transformed human cells.


Establishment of human malignant melanoma clonal cell lines that secrete ectopic adrenocorticotropin.


Physiotherapy in the treatment of leprosy.


Neuropathy in chronic renal disease.

Neuropathy in chronic renal disease.


Catecholamine-containing neurones and electrical self-stimulation. 1. A review of some data.


Membrane resistance changes underlying the bipolar cell response in the carp retina.


Bipolar-amacrine transmission in the carp retina.


Viral hepatitis. Report of a WHO scientific group.


Neutralization tests for foot-and-mouth disease, equine rhino-, porcine entero-, Aujeszky- and rhinopneumonitis viruses. Comparison of results obtained by a simple microculture plaque reduction test and various traditional tests.


Comparison of isometric exercise and angiotensin infusion as stress test for evaluation of left ventricular function.


Coexistent congenital cytomegalovirus and toxoplasmosis in a stillborn.


Attempts to produce tumors in mice with CELO virus.


On the kinetics of poliovirus-specific protein and RNA synthesis in poliovirus-infected cells.


Electron microscopic study of Sindbis virus morphogenesis.


Isolation and characterization of reoviruses isolated from sheep.


Pain: a current view of neural mechanisms.


Mössbauer effect in rubredoxin. Determination of the hyperfine field of the iron in a simple iron-sulphur protein. 1. Rubredoxin isolated from the green photosynthetic bacterium Chloropseudomonas ethylica was similar in composition to those from anaerobic fermentative bacteria. Amino acid analysis indicated a minimum molecular weight of 6352 with one iron atom per molecule. 2. The circular-dichroism and electron-paramagnetic-resonance spectra of Ch. ethylica rubredoxin showed many similarities to those of Clostridium pasteurianum, but suggested that there may be subtle differences in the protein conformation about the iron atom. 3. Mössbauer-effect measurements on rubredoxin from Cl. pasteurianum and Ch. ethylica showed that in the oxidized state the iron (high-spin Fe(3+)) has a hyperfine field of 370+/-3kG, whereas in the reduced state (high-spin Fe(2+)) the hyperfine field tensor is anisotropic with a component perpendicular to the symmetry axis of the ion of about -200kG. For the reduced protein the sign of the electric-field gradient is negative, i.e. the ground state of the Fe(2+) is a [unk] orbital. There is a large non-cubic ligand-field splitting (Delta/k=900 degrees K), and a small spin-orbit splitting (D~+4.4cm(-1)) of the Fe(2+) levels. 4. The contributions of core polarization to the hyperfine field in the Fe(3+) and Fe(2+) ions are estimated to be -370 and -300kG respectively. 5. The significance of these results in interpretation of the Mössbauer spectra of other iron-sulphur proteins is discussed.


Purine ribonucleotide biosynthesis, interconversion and catabolism in mouse brain in vitro. The relative rates of the synthetic, interconversion and catabolic reactions of purine metabolism in chopped mouse cerebrum were studied. The rates of incorporation of [(14)C]adenine and [(14)C]hypoxanthine into purine ribonucleotides were much less than the potential activities of adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase, and the rates of incorporation were stimulated by the addition of guanosine to the incubation mixture. The availability of ribose phosphates may be a limiting factor for the formation of ribonucleotides from purine bases. The rate of incorporation of [(14)C]adenosine into purine ribonucleotides was at least seven- to eight-fold higher than that of adenine. The radioactivity in adenine ribonucleotides synthesized from adenine and hypoxanthine was about 100- and ten-fold respectively higher than that in the radioactive guanine ribonucleotides. The conversion of inosinate into guanine ribonucleotides was probably limited by the amount of inosinate available, and the conversion of adenine ribonucleotides into guanine ribonucleotides was probably limited by the activity of adenylate deaminase. The rate of catabolism of [(14)C]adenosine was low in comparison with its rate of utilization for ribonucleotide synthesis. A fraction of the [(14)C]hypoxanthine was catabolized to xanthine and urate. [(14)C]Guanine was completely converted into xanthine, mostly by the guanine deaminase that was released during incubation of chopped mouse cerebrum.


Release of creatine phosphokinase from muscle. II. Effect of multiple doses of polymyxin B or compound 48-80 on increases in plasma enzyme levels.


Influence of pH on human renin activity with different substrates: role of substrate denaturation.


Histone phosphatase and cyclic nucleotide-stimulated protein kinase from human lymphocytes. 1. Extracts of human peripheral blood lymphocytes contained a histone phosphatase that catalysed the release of P(i) from phosphorylated whole thymus histone. 2. Stimulation of the phosphatase was obtained by concentrations of KCl and NaCl of up to 75mm, and by MgCl(2); CaCl(2) inhibited the enzymic activity. 3. In the absence of MgCl(2), phosphoenol-pyruvate inhibited histone phosphatase activity; this inhibition could be partially reversed by adding MgCl(2) to assays. 4. Lymphocyte extracts contained a protein kinase activity which was maximally stimulated by 1mum-cyclic AMP (adenosine 3':5'-cyclic monophosphate) or by 0.1mm-cyclic GMP (guanosine 3':5'-cyclic monophosphate). 5. Incubation of the enzyme with histone in the absence of ATP or MgCl(2) resulted in the dissociation of the enzyme into a lower-molecular-weight species that was not stimulated by cyclic AMP. This effect could be prevented if ATP and MgCl(2) were present in reaction mixtures before histone and enzyme were allowed to interact. 6. Cyclic AMP also dissociated the kinase into a lower-molecular-weight species. 7. In the presence of 1mum-AMP, half-maximal activities were obtained with 0.92mm-MgCl(2), 6.0mum-ATP and 0.23mg of whole thymus histone/ml.


Fractionation of nuclei from brain by zonal centrifugation and a study of the ribonucleic acid polymerase activity in the various classes of nuclei. 1. The nuclei of the cells of the whole rat brain have been fractionated in a B-XIV zonal rotor with a discontinuous gradient of sucrose. Five fractions were obtained. Zone (I) contained neuronal nuclei (70%) and astrocytic nuclei (23%). Zone (II) contained astrocytic nuclei (81%) and neuronal nuclei (15%). Zone (III) contained astrocytic nuclei (84%) and oligodendrocytic nuclei (15%). Zone (IV) contained oligodendrocytic nuclei (92%) and zone (V) contained only oligodendrocytic nuclei. 2. The content of DNA, RNA and protein per nucleus was determined for each zone. Although the amount of DNA per nucleus is constant (7pg) the RNA varies from 4.5 to 2.5pg/nucleus and the protein from 38 to 17.6pg/nucleus. The neuronal nuclei have the greatest amounts of protein. The oligodendrocytic nuclei have the least content of RNA and protein. 3. The effects of pH, ionic strength, and Mg(2+) and Mn(2+) concentration on the activity of the nuclear system for synthesis in vitro of RNA have been investigated for unfractionated nuclei. From these studies a standard set of conditions for the assay of nuclear RNA polymerase has been established. 4. The activity of the RNA polymerase in each of the zonal fractions has been determined in the presence and in the absence of alpha-amanitin. Zone (II) is the most active, followed by zone (I). The nuclei of zones (IV) and (V) have comparable activity, which is 40% of that of zone (II). 5. The extent of incorporation of each of the four labelled nucleoside triphosphates by the nuclei from each zone has been measured. These values have been used to calculate the base composition of the RNA synthesized in vitro in each class of nucleus. 6. The effect of changes in the condition of assay of RNA polymerase in the different classes of nuclei has been investigated. Significant differences in the response to concentrations of metal ions and ammonium sulphate have been observed. 7. Homopolymer formation in each zone of brain nuclei has been determined. The extent of formation of the four homopolymers roughly parallels the RNA polymerase activity.


Two forms of UDPglucose-4-epimerase from mammalian liver.


Comparative specificity of prenyltransferase of pig liver and pumpkin with respect to artificial substrates.


Partial prufication and properties of the cyclic AMP and the cyclic GMP phosphodiesterases of bovine liver.


Purification of a soluble adenosine 5'-phosphosulphate sulphohydrolase from bovine liver.


Isolation by affinity chromatography of neutral proteinase from Clostridium histolyticum.


Effect of prostaglandins E 1 , E 2  and A 2  on vascular resistance and responses to noradrenaline, nerve stimulation and angiotensin in the dog hindlimb. 1. The relationship between the vasodilator and inhibitory effects of prostaglandin E(1) (PGE(1)), E(2) and A(2) on responses to nerve stimulation, noradrenaline and angiotensin was evaluated in the dog hindlimb preparation.2. PGE(1) and PGE(2) were equipotent as vasodilators in the hindlimb; however, PGE(1) was much more potent as an inhibitor of vasoconstrictor responses to nerve stimulation, noradrenaline and angiotensin.3. PGA(2) and PGE(2) were approximately equal as inhibitors of vasoconstrictor responses to noradrenaline, nerve stimulation and angiotensin; however, PGE(2) was far more potent as a vasodilator.4. Since there is no relationship between the vasodilator and inhibitory effects of PGE(1), E(2) and A(2), and since the inhibitory effect of PGA(2) was present at a time when hindlimb perfusion pressure had returned to control value, it is concluded that the inhibitory action is probably not the result of a physiological antagonism.5. Since each prostaglandin inhibited responses to nerve stimulation and noradrenaline to approximately the same extent and responses to angiotensin were also inhibited, it is suggested that these agents antagonize vasoconstrictor responses by a nonspecific depressant effect on smooth muscle cells.


The effects of some phosphodiesterase inhibitors on the conductance of the perfused vascular beds of the chloralosed cat. 1. The vasoactivity of a number of phosphodiesterase inhibitors in the auto-perfused splanchnic region and hindquarters of the cat have been investigated.2. Theophylline, dipyridamole and 4-(3,4-dimethoxybenzyl)-2-imidazolidinone (Ro 7-2956) and its analogues were more potent vasodilators in the splanchnic region than in the hindquarters, whereas papaverine was a potent vasodilator in both these vascular beds.3. The vasodilator response to the phosphodiesterase inhibitors in the splanchnic region and hindquarters was not susceptible to beta-adrenoceptor blockade.4. Infusion of dibutyryl cyclic AMP into the superior mesenteric artery increased vascular conductance of the splanchnic region. This effect was enhanced by Ro 20-1724 in doses below those in which this compound affected conductance.5. These results are consistent with the hypothesis that accumulation of cyclic AMP in vascular smooth muscle mediates the vasodilator response to phosphodiesterase inhibitors.


Augmentation of drug-induced blood pressure increases in rats by amorbarbital. The effects of amobarbital anaesthesia on blood pressure responses to angiotensin and noradrenaline were determined in rats with chronically implanted venous and aortic catheters. Responses were tested twice on the same day in each rat, first in the conscious state and then during anaesthesia. Blood pressure rises produced by noradrenaline and angiotensin were larger when the rats were anaesthetized with amobarbital than when they were conscious. This enhancement was not characteristic of general anaesthesia since urethane, instead of enhancing, depressed the pressor responses.Blockade of acetylcholine receptors with atropine also increased the response to pressor drugs. However, amobarbital in anaesthetic doses did not affect the response to acetylcholine, indicating that removal of compensatory parasympathetic reflexes was not a major cause of its enhancing effect. The effects of amobarbital on arterial pressure and heart rate were similar to those of the ganglion blocking drug pentolinium. When pressor responses were increased by amobarbital, autonomic ganglia were depressed as indicated by the loss of the response to nicotine. These findings suggest that amobarbital enhances blood pressure responses to noradrenaline and angiotensin by blocking autonomic ganglia.


The effect of cold storage on the inhibitory action of isoprenaline, phenylephrine and nicotine on the mechanical and membranal activities of guinea-pig taenia caecum. 1. The effects of prolonged cold storage on the mechanical and membranal responses to stimulation of alpha- and beta-adrenoceptors by phenylephrine and isoprenaline, respectively, were studied on the guinea-pig taenia caecum.2. Cold storage invariably caused a decrease in the resting membrane potential, and this effect was enhanced as the duration of treatment was prolonged.3. After cold storage (18 days) the tissue potassium ion content (89.7 +/- 1.7 mmol/kg wet wt.) was decreased to 30.5 +/- 1.9 mmol/kg wet wt. whereas that for sodium (69.2 +/- 1.4 mmol/kg wet wt.) increased to 134.0 +/- 2.3 mmol/kg wet wt.4. In the fresh preparations, phenylephrine (1 and 2 muM) caused a cessation of spontaneous action potentials, accompanied by hyperpolarization of the membrane and relaxation of the muscle. These effects were markedly diminished after 18 days of cold storage. Isoprenaline (1 and 2 muM) also blocked the action potentials and caused a concomitant muscle relaxation, but in most cases the hyperpolarization was not observed. After 14 days of cold storage these mechanical and membranal changes associated with isoprenaline treatment were not demonstrable in most preparations.5. Nicotine (5 muM and 50 muM) produced a biphasic effect, cessation of the action potential, hyperpolarization and subsequent relaxation followed by a long lasting depolarization, an accelerated discharge of action potentials and an increase in muscle tension. After a few days of cold storage the hyperpolarization effect disappeared but the intensity of the long-lasting depolarization as well as the contractile effects were increased. After cold storage for more than 7 days, nicotine did not affect mechanical or electrical activity.6. Dibutyryl 3'5' cyclic AMP (1 muM to 500 muM) failed to affect the mechanical and electrical activities of taenia caecum.7. Phenylephrine and isoprenaline had no effect on the high potassiumdepolarized taenia.8. These observations suggest that the electro-mechanical effect of an alpha-adrenoceptor stimulant on the guinea-pig taenia caecum is more resistant to cold treatment than that of a beta-adrenoceptor stimulant. This inhibitory system of stimulation of both a- and 8-receptors of guinea-pig taenia caecum may react by different mechanisms. The results also demonstrate that cold storage itself changes the membrane permeability to ions and the tissue ion content (Na+ and K+) of smooth muscle of guinea-pig taenia caecum.


The effect of N6,O2'-dibutyryl adenosine 3':5'-cyclic monophosphate on noradrenaline synthesis in isolated superior cervical ganglia.


Studies on the mode of action of cyclic 3'5'-AMP and prostaglndin E 2  on rat gastric acid secretion and mucosal blood flow.


The effect of some synthetic analogues of ACTH on the metabolism of biogenic amines in the rat brain.


Estimation of angiotensin II blood concentrations in hypertensive rats.


Effect of chlorpromazine on REM rebound in normal volunteers.


Recurrent hyperkalaemia due to selective aldosterone deficiency: correction by angiotensin infusion. A patient with recurrent weakness and blurring of consciousness associated with hyperkalaemia due to aldosterone deficiency is reported. The plasma concentrations of renin, angiotensin II, and aldosterone were low and did not increase during sodium deprivation. Blood angiotensin I was also low while renin-substrate concentration was normal. Infusion of angiotensin produced a distinct rise in plasma aldosterone. The patient was treated successfully with fludrocortisol.The results support the concept that the renin-angiotensin system is an important regulator of aldosterone secretion and that in the syndrome of acquired selective hypoaldosteronism the primary abnormality may be a deficiency of renin. It is suggested that a selective lack of aldosterone should be considered in all cases of otherwise unexplained hyperkalaemia.


Evidence that pepsitensin is angiotensin I.


Selective suppression of rapid eye movement sleep (REM) by fusaric acid, an inhibitor of dopamine- -oxidase.


Electron microscopic observations of neocarzinostatin treated Burkitt lymphoma cells.


The finding of viral particles in spontaneous mammary adenocarcinoma in rats.


Co-existence of a guanidine-dependence and of a thiopyrimidine dependence in the same strain of poliovirus.


Preparation and biological activity of some anthyridine derivatives.


Adenoviral infection in foals.

Adenoviral infection in foals.


Cerebral malformation in fetal lambs from a bluetongue-enzootic flock.


Characterization of the 4-acetamido-4,6-dideoxyhexoses from Escherichia coli strains.


Properties of 5-phosphoribosyl-1-pyrophosphate amidotransferase from human lymphoblasts.


Human glutamine phosphoribosylpyrophosphate amidotransferase. Kinetic and regulatory properties.


Genetic regulation of aryl hydrocarbon hydroxylase induction. V. Specific changes in spin state of cytochrome P 450  from genetically responsive animals.


Dissociation and activation of adenosine 3',5'-monophosphate-dependent and guanosine 3',5'-monophosphate-dependent protein kinases by cyclic nucleotides and by substrate proteins.


Protein kinase modulator from lobster tail muscle. I. Stimulatory and inhibitory effects of the modulator on the phosphorylation of substrate proteins by guanosine 3',5'-monophosphate-dependent and adenosine 3',5'-monophosphate-dependent protein kinases.


Protein kinase modulator from lobster tail muscle. II. Effects of the modulator on holoenzyme and catalytic subunit of guanosine 3',5'-monophosphate-dependent and adenosine 3',5'-monophosphate-dependent protein kinases.


Phenylalanine hydroxylase in cultured hepatoxytes. I. Hormonal control of enzyme levels.


Separate oxidase and reductase reaction sites on cytochrome c demonstrated with purified site-specific antibodies.


Insulin-receptor interaction in the obese-hyperglycemic mouse. A model of insulin resistance.


Studies on the mechanism of action of uridine diphosphate galactose 4-epimerase. II. Substrate-dependent reduction by sodium borohydride.


Enzyme secretion mediated by the epinephrine  -receptor in rat parotid slices. Factors governing efficiency of the process.


Potassium release mediated by the epinephrine  -receptor in rat parotid slices. Properties and relation to enzyme secretion.


Inhibition of ribosomal ribonucleic acid maturation in Novikoff hepatoma cells by 5-fluorouracil and 5-fluorouridine.


The mechanism of the water expulsion vesicle of the ciliate Tetrahymena pyriformis. Analysis of high-speed (150 frames/sec) cinematographs of the filling and expulsion of the water expulsion vesicle of Tetrahymena pyriformis shows that the vesicle fills as water is pumped into it by contractions of at least four ampullary sacs which are continuous with the endoplasmic reticulum. When filled, the vesicle is pressed against its two excretory pores by cyclotic movements of the cytoplasm. This pressure closes the apertures of the ampullae, preventing backflow from the vesicle into them, and also spreads the pellicle of and at the pore, thereby stretching and rupturing the pore-sealing membrane. The vesicle is then invaginated by the cytoplasmic pressure, driving fluid out of the pore. The pore-sealing membrane then reforms, apparently by constriction, and the vesicle is again filled. Electron micrographs show that crisscrossed pore-microtubules extend from the pore to the openings of the ampullae, anchoring the vesicle in place. Each pore is surrounded by a stack of at least 11 ring-microtubules, to which the anchoring pore-microtubules are attached. The pore-microtubules appear to exert tension which assists in spreading the pore, aiding cyclotic pressures in rupturing the pore-sealing membrane. A possible mechanism for the cyclotic pressure and ampullary contraction is proposed.


Nematosomes in the rat trigeminal ganglion.


Ultrastructural studies of cell-virus interaction in reptilian cell lines. I. Intramitochondrial viruses in "C-type" virus-producing VSW cells.


Transplantation immunity to simian virus 40-transformed cells in tumor-bearing mice. I. Development of cellular immunity to simian virus 40 tumor-specific transplantation antigens during tumorigenesis by transplanted cells.


Susceptibility of mammalian (hamster) cell culture to infection with cellular and cell-free type II leukosis (Marek's disease) virus.


Transplantation immunity to simian virus 40-transformed cells in tumor-bearing mice. II. Evidence for macrophage participation at the effector level of tumor cell rejection.


Enhanced tumor growth in animals pretreated with complete Freund's adjuvant.


Effect of treatment of C57BL-6 mice with the methanol extraction residue fraction of BCG on leukemogenesis induced by the radiation leukemia virus.


Activation of a type-C RNA virus from tumors induced by rat kidney cells transformed by a chemical carcinogen.


Preparation of pure cultures of tumor macrophages.


Morphogenesis of C-type and A-type particles in cells infected by an arbovirus.


Brain tumors induced by Rous sarcoma virus, Schmidt-Ruppin strain. 3. Morphology of brain tumors induced in adult mice.


Effect of CCNU (NSC-79037) on bronchogenic carcinoma.


A case of leprous nodular interstitial myositis.


Autoxidation of polyunsaturated fatty esters on silica.


Malignant lymphoma. 1.

Malignant lymphoma. 1.


Parasympathetic control of the heart.

Parasympathetic control of the heart.


The PARA-adenoviruses.

The PARA-adenoviruses.


Annular nucleolus in Leydig cells of mouse.


Electron microscopic study on the thyroid gland of the salamander Hynobius nebulosus in the breeding season. I. Observation under normal conditions.


Electron microscopic study on the thyroid gland of the salamander Hynobius nebulosus in the breeding season. II. Observation under experimental conditions.


Synaptic junction development in the spinal cord of an amphibian embryo: an electron microscope study.


The effect of enzymes on extracellular connective tissue components in the developing chick aorta.


Ultrastructural study of virilizing adrenocortical adenoma.


Enterovirus surveillance following a community-wide oral poliovirus vaccination program: a seven-year study.


Acute respiratory disease in military trainees: the adenovirus surveillance program, 1966-1971.


Significance and treatment of lymph node metastases of malignant mucous and salivary gland tumors.


Immunoglobulin response and viremia in dengue-vaccinated gibbons repeatedly challenged with Japanese encephalitis virus.


Superior vena caval syndrome: scintiphotographic evaluation of response to radiation therapy.


Inferior phrenic artery collateralization in hepatic artery occlusion.


Mixed mesodermal sarcoma of the uterus.

Mixed mesodermal sarcoma of the uterus.


An attempt to clarify the immunobiological significance of Mott's cells.


Inhibition of poliovirus multiplication by a normal horse serum. I. Characterization of virus-inhibitor interaction.


Oxidative phosphorylation in intact bacteria.


The pressor effect of angiotensin II in pregnant sheep.


Genetic and biochemical studies of the adrenal lipid depletion phenotype in mice.


Genetic relationships between the multiple alcohol dehydrogenases of maize.