Sudden and unexpected deaths in infants: histology and virology. The histological appearance of lungs of children whose death had been sudden and unexpected has been studied in relation with the viruses found at necropsy. Fifty-one children were investigated and in 33 the lungs had the histological appearance widely accepted as characteristic of the condition previously termed "cot death." Sixteen had the histological appearance of lymphocytic bronchiolitis, and it was from this group only that viruses were isolated. The viruses isolated were those associated with respiratory infections. Two patients showed the histological appearance of bacterial bronchopneumonia. Viruses are probably responsible for a substantial proportion of unexpected sudden deaths in infancy.


Actinomycin D for Wilm's tumour.

Actinomycin D for Wilm's tumour.


Antibiotic sensitivity of Clostridium perfringens and L-forms of C. perfringens induced by bacteriocin.


Radioimmunoassay. Practices and pitfalls.


Use of angiotensin II antagonists in experimental hypertension.


Importance of potassium in the regulation of aldosterone production.


Effects of a specific competitive antagonist of angiotensin II on arterial pressure and adrenal steroid secretion in dogs.


Physiological receptors as physicochemical entities.


A model of the human circulation. Regulation by autonomic nervous system and renin-angiotensin system, and influence of blood volume on cardiac output and blood pressure.


Kinetic colorimetric measurement of serum lactate dehydrogenase activity.


Simultaneous competitive protein binding assay for cortissol, cortisone, and prednisolone in plasma, and its clinical application.


Hippocampal epilepsy produced by ouabain. Studies of cerebral circulation and ionic metabolism.


Relationship of epithelial membrane potentials to corneal potential.


Ion pumps in the cornea and their stimulation by epinephrine and cyclic-AMP.


Lungfishes and amphibians: endocrine adaptation and the transition from aquatic to terrestrial life.


Prolactin and osmoregulation in fishes.

Prolactin and osmoregulation in fishes.


Regulation of electrolyte balance in marine birds with special reference to the role of the pituitary-adrenal axis in the duck (Anas platyrhynchos).


Electron spin resonance studies on aqueous methylene blue solutions.


Maori art and pseudo-hypoparathyroidism--a medical contribution to prehistoric anthropology.


Treatment of oral syndrome obesity with non-traditional appetite control plan.


Herpesviruses and cancer: introduction.

Herpesviruses and cancer: introduction.


Bone case of the month.

Bone case of the month.


Day care: Pandora's box?

Day care: Pandora's box?


Role of cyclic adenosine 3',5'-monophosphate in the in vivo expression of the galactose operon of Escherichia coli. Studies of levels of galactokinase in Escherichia coli with mutations affecting synthesis of, or response to, cyclic adenosine 3',5'-monophosphate show that this nucleotide does not play a major role in expression of the galactose operon, causing at most a twofold stimulation. The discrepancy between our in vivo results and the marked stimulation by cyclic adenosine 3',5'-monophosphate in in vitro systems indicates that current cell-free systems lack a factor which allows efficient expression of the galactose operon even in the absence of cyclic adenosine 3',5'-monophosphate or of the binding protein for this nucleotide.


Cyclic adenosine 3',5'-monophosphate in Escherichia coli. The concentration of cyclic adenosine 3',5'-monophosphate (c-AMP) in Escherichia coli growing on different sources of carbon was studied. Cultures utilizing a source of carbon that supported growth relatively poorly had consistently higher concentrations of c-AMP than did cultures utilizing sugars that supported rapid growth. This relationship was also observed in strains defective in c-AMP phosphodiesterase and simultaneously resistant to catabolite repression; in such strains the c-AMP concentration was slightly higher for several sources of carbon tested. Cultures continued to synthesize c-AMP and secreted it into the medium, under conditions that brought about an inhibition of the intracellular accumulation of the cyclic nucleotide. Transient repression of the synthesis of beta-galactosidase was not associated with an abrupt decrease in the cellular concentration of c-AMP.


Genetic mutations affecting the respiratory electron-transport system of the photosynthetic bacterium Rhodopseudomonas capsulata. Alternative energy-converting systems permit the nonsulfur purple photosynthetic bacterium Rhodopseudomonas capsulata to grow either with light or (dark) respiration as the source of energy. Respiratory mutants, unable to grow aerobically in darkness, can be readily isolated and the defective step(s) in their respiratory mechanisms can be identified by study of biochemical activities in membrane fragments derived from photosynthetically grown cells. Such analysis of appropriate mutants and revertants permits construction of a model for the respiratory electron-transport system of the wild type. The results obtained indicate differential channeling of electrons derived from succinate and reduced nicotinamide adenine dinucleotide, and are interpreted in terms of a branched electron-transport scheme. The scheme provides a guide for further, more refined analysis of the respiratory mechanisms through biochemical genetic approaches, and several of the mutants isolated can be exploited for investigation of unsolved problems relating to interactions between respiratory and photosynthetic electron transport and the mechanism of inhibition of bacteriochlorophyll synthesis by molecular oxygen.


Herpesvirus and the frog renal adenocarcinoma.


Herpesviruses saimiri and ateles--their role in malignant lymphomas of monkeys.


Possible role of Herpesvirus hominis, type 2, in human cervical cancer.


In vitro cell transformation by herpesviruses.


Role of herpesviruses in malignant lymphoma in rabbits.


Isolation and characterization of plasmid deoxyribonucleic acid from Streptococcus mutans. A small plasmid with a molecular weight of approximately 3.0 x 10(6) and present to the extent of about 16 copies per chromosomal genome equivalent was isolated from Streptococcus mutans strain LM-7.


Role of Epstein-Barr virus in infectious mononucleosis and malignant lymphomas in man.


Glucose fermentation products in Ruminococcus albus grown in continuous culture with Vibrio succinogenes: changes caused by interspecies transfer of H 2 . The influence of a H(2)-utilizing organism, Vibrio succinogenes, on the fermentation of limiting amounts of glucose by a carbohydrate-fermenting, H(2)-producing organism, Ruminococcus albus, was studied in continuous cultures. Growth of V. succinogenes depended on the production of H(2) from glucose by R. albus. V. succinogenes used the H(2) produced by R. albus to obtain energy for growth by reducing fumarate in the medium. Fumarate was not metabolized by R. albus alone. The only products detected in continuous cultures of R. albus alone were acetate, ethanol, and H(2). CO(2) was not measured. The only products detected in the mixed cultures were acetate and succinate. No free H(2) was produced. No formate or any other volatile fatty acid, no succinate or other dicarboxylic acids, lactate, alcohols other than ethanol, pyruvate, or other keto-acids, acetoin, or diacetyl were detected in cultures of R. albus alone or in mixed cultures. The moles of product per 100 mol of glucose fermented were approximately 69 for ethanol, 74 for acetate, 237 for H(2) for R. albus alone and 147 for acetate and 384 for succinate for the mixed culture. Each mole of succinate is equivalent to the production of 1 mol of H(2) by R. albus. Thus, in the mixed cultures, ethanol production by R. albus is eliminated with a corresponding increase in acetate and H(2) formation. The mixed-culture pattern is consistent with the hypothesis that nicotinamide adenine dinucleotide (reduced form), formed during glycolysis by R. albus, is reoxidized during ethanol formation when R. albus is grown alone and is reoxidized by conversion to nicotinamide adenine dinucleotide and H(2) when R. albus is grown with V. succinogenes. The ecological significance of this interspecies transfer of H(2) gas and the theoretical basis for its causing changes in fermentation patterns of R. albus are discussed.


Purinergic autonomic neurones.

Purinergic autonomic neurones.


Biochemical transformation of L-cells with ultraviolet-irradiated herpes simplex virus.


Comparative study of two RD 114 virus-indicator cell lines, KC and KB.


Enzymes in bovine milk: a review.

Enzymes in bovine milk: a review.


Patterns of antibodies to Epstein-Barr virus-induced early antigens in Burkitt's lymphoma. Comparison of dying patients with long-term survivors.


Spontaneous tumors in C3H-A vy  and C3H-A vy fB mice: high incidence in the United States and low incidence in Australia.


Lifespan and incidence of cancer and other diseases in selected long-lived inbred mice and their F 1  hybrids.


Lysis of tumor cells by antibody and complement. 1. Lack of correlation between antigen content and lytic susceptibility.


Induction of lymphocytic leukemia, lymphosarcoma, reticulum cell sarcoma, and osteogenic sarcoma in the Syrian golden hamster by oncogenic DNA simian virus 40.


Carcinoembryonic antigen in normal human saliva.


Thymic dependency of tumor-induced immunodepression.


Adjuvants for a tumor-specific vaccine: Bordetella pertussis or mycobacterial cell walls attached to oil droplets.


Immunosuppression and experimental lymphoma.


4-Methanesulfonyloxybutanol: hydrolysis of busulfan.


Electrophysiologic effects of diphenidol in isolated cardiac tissue.


Blockade of adrenergic potentiating effect of angiotensin by 1-Sar-8-Ala-angiotensin II.


Pharmacology of cyanate. II. Effects on the endocrine system.


Heredity and cancer in man.

Heredity and cancer in man.


The funding crisis: an opportunity for growth.


The familial dyslipoproteinemias.

The familial dyslipoproteinemias.


The background and promise of tumor virology, immunology, and chemotherapy and a challenge to radiotherapy. Janeway Memorial lecture.


Reliability of skeletal age assessments.


The enlarge foramen ovale.

The enlarge foramen ovale.


Simple vulvectomy--a 10-year review.

Simple vulvectomy--a 10-year review.


Immune response in families of children with acute lymphoblastic leukaemia.


Effect of ADP-induced aggregation on  32 PO 4  incorporation into phosphatidic acid and the phosphoinositides of rabbit platelets.


The role of dermal blood vessels in the pathogenesis of malignant atrophic papulosis (Degos' disease). A study of two cases using enzyme histochemical, fibrinolytic, electron-microscopical and immunological techniques.


Human growth hormone: a comparison of the results of plasma and serum assays using double-antibody techniques.


Studies on differentiation of avian pox viruses.


Adenovirus infection and disease. II. Histopathology of natural and experimental disease.


Adenovirus infection and disease. I. Some characteristics of an isolate from chickens in Indiana.


A procedure for the determination of a renin inhibitor in human plasma.


Effect of ampicillin on the urinary output of steroidal hormones in pregnant and non-pregnant women.


Characterization of two reoviruses isolated from turkeys with infectious enteritis.


Purine metabolism in primitive erythrocytes.


Biochemical studies in the vole, Microtus montanus. II. The effects of a Trypanosoma brucei gambiense infection on the diurnal variation of hepatic glucose-6-phosphatase and liver glycogen.


Biochemical studies in the vole, Microtus montanus. I. The daily variation of hepatic glucose-6-phosphatase and liver glycogen.


Hydrolytic enzymes of the hepatopancreas of the ascidian, Pyura stolonifera.


Isolation and characterization of viruses associated with transmissible enteritis (bluecomb) of turkeys.


Isolation and characterization of a virus associated with arthritis of chickens.


Wax ester biosynthesis by isolated membrane fractions from calanoid copepods.


Enzymes in facultative anaerobiosis of molluscs. I. Malic enzyme of oyster adductor muscle.


Arginyl-transfer ribonucleic-acid synthetase from Bacillus stearothermophilus. Purification, properties and mechanism of action.


Structure of the parasporal inclusion of Bacillus thuringiensis Berliner: characterization of a repetitive subunit.


Studies on the interaction of ligands with phosphorylase b using a spin-label probe.


Energy-dependent changes in membranes of Rhodospirillum rubrum chromatophores as measured by 8-anilino-naphthalene-1-sulfonic acid.


The subunit structure of the pyruvate-dehydrogenase complex from Escherichia coli K-12. The core complex.


Benzene metabolism of Moraxella species.


Effect of strychnine on the primary evoked response and on the corticofugal reflex discharge.


Characterization of the native and denatured conformations of tRNA Ser and tRNA Phe from yeast.


Reflexes involving triceps surae from the ankle joint of the cat.


Peripheral neuropathies in diseases of internal organs.


The development of the chick tertiary bronchus. II. The origin of the surface lining system.


The development of the chick tertiary bronchus. I. General development and the mode of production of the osmiophilic inclusion body.


Corneal immune response to Herpes simplex virus antigens.


Increased metabolic clearance of cortisol by stress-susceptible swine.


Prevention of MIF activity by agents known to increase cellular cyclic AMP.


The effects of tetanus toxin on the motor end-plates of the mouse. An electron microscopic study.


Follow-up after hydatidiform mole: studies using radioimmunoassay for urinary human chorionic gonadotrophin (HCG).


Fusion of erythrocytes by Sendai virus studied by immuno-freeze-etching. Extensive fusion of human erythrocytes agglutinated by Sendai virus was observed after 30 s of incubation at 37 C. Electron microscopy of thin sections failed to reveal the presence of virions, viral fragments, or discrete viral antigens reactive with ferritin-labeled antibody at the sites of fusion. Immuno-freezeetching of membrane surfaces demonstrated the dispersal of viral envelope antigens from what appeared to be original sites of viral attachment. Virus-induced clustering of membrane glycoproteins was interpreted as resulting from interaction of viral antigens with membrane receptor proteins and forming the structural basis for fusion of membranes with one another.


Virus-induced proteins in pseudorabies-infected cells. I. Acid-extractable proteins of the nucleus. Three basic proteins have been identified in chromatin preparations from pseudorabies virus-infected cells. They appear to be virus specified and are similar in size and charge to host histones; one difference however is that they contain tryptophan. All are produced by 3 h postinfection, and two (IP II and III) seem to be arginine rich. Three similar proteins are also found in herpes simplex MP 17-infected cells, and two of these co-electrophorese with two of the pseudorabies proteins. Partially purified preparations of pseudorabies virus contain low amounts of all three proteins.


Lack of sequence homology between the 70S RNA of various RNA tumor viruses and the DNA of simian virus 40 or polyoma virus. No significant hybridization was detected of DNA from simian virus 40 or polyoma virus, and of 70S RNA from avian myeloblastosis virus, murine leukemia virus (Rauscher), murine sarcoma virus (Kirsten), RD-114B, simian sarcoma virus-1, or Mason-Pfizer virus.


Polioviruses, coxsackieviruses, and echoviruses comparison of the genomes by RNA hybridization. Hybridization of single-stranded RNA from virions of human enteroviruses with denatured double-stranded RNA from infected cells indicates that a minimum of about 5% of the genome is shared by these viruses. Polynucleotide sequence relationships, furthermore, are consistent with the biologic classification into polioviruses, coxsackieviruses groups A and B, and echoviruses. In general, about 30 to 50% of the nucleotide sequences are shared by different serotypes of virus within each of these major groups, whereas among different groups less than 20% homology is observed. Coxsackievirus B4 appears to be more closely related to echoviruses than to group A coxsackieviruses, whereas polioviruses are only distantly related to any of the other agents.


RNA synthesis in cells infected with herpes simplex virus. VII. Control of transcription and of transcript abundancies of unique and common sequences of herpes simplex virus 1 and 2. Analysis of the kinetics of hybridization in liquid of labeled herpes simplex virus (HSV) 1 and 2 DNAs with excess unlabeled RNA extracted at 2 (early) and 8 (late) h postinfection revealed the following. (i) The RNA transcripts present in the HSV-1-infected cells at 2 and 8 h postinfection are complementary to 44 and 48% of HSV-1 DNA. The RNA transcripts present in the HSV-2-infected cells at 2 and 8 h postinfection are complementary to 21 and 50% of HSV-2 DNA. (ii) The transcripts present in 2-h HSV-1- or HSV-2-infected cells treated with cycloheximide are complementary to 44 and 45% of the respective DNAs. (iii) The RNA transcripts present in the HSV-1-infected cells at 2 h postinfection and in HSV-2-infected cells at 8 h postinfection form 2 classes, abundant and scarce, differing in molar concentrations. The RNA transcripts present in the HSV-2-infected cells at 2 h postinfection form only one abundance class. (iv) The transcripts present in the HSV-1-infected cells at 8 h postinfection are complementary to 24% of HSV-2 DNA and therefore 50% of the transcribed HSV-1 sequences are shared by the two viruses. Of the RNA sequences complementary to HSV-2 DNA, 13% arise from HSV-1 templates specifying abundant RNA and 11% arise from HSV-1 templates specifying scarce RNA. Thus, the DNA sequences shared in common by HSV-1 and HSV-2 DNAs constitute 71% of the HSV-1 templates specifying abundant RNA and 39% of sequences specifying scarce RNA.


Radioimmunoassay for avian C-type virus group-specific antigen: detection in normal and virus-transformed cells. A radioimmunoassay has been developed for detection of avian C-type virus (30,000 mol wt) group-specific (gs) antigen. The method is 10- to 1,000-fold more sensitive than immunological methods previously available. By the radioimmunoassay technique, normal chicken embryo cells, which have previously been classified as gs negative or weakly gs positive, contain clearly detectable amounts of gs antigen. The assay has been used to study the effect of chemical induction and superinfection by mammalian C-type viruses on the expression of avian gs antigen in mammalian cells nonproductively transformed by avian sarcoma viruses.


Complementary RNA species isolated from vesicular stomatitis (HR strain) defective virions. The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectable complementary (plus) strand RNA. In contrast, a preparation of a heat-resistant (HR) strain of VSV containing defective virions was found to contain both plus (21%) and minus strand RNA, present in several distinct size classes. It was found that the RNA in the HR virion preparation was at least 94% single-stranded and principally (96%) in ribonucleoprotein complexes. On extraction the plus and minus strand RNA species partially annealed to give a population of double- and multistranded RNA species. A small amount of RNA polymerase activity was associated with the HR defective virus preparation.


Electrophoretic differences in the capsid proteins of simian virus 40 plaque mutants. The structural proteins of three mutants of simian virus 40 (SV40) which differ in plaque size, temperature sensitivity, oncogenicity, host cell restriction, and immunological properties were studied. The polypeptide components of these SV40 strains could not be distinguished by their polyacrylamide gel electrophoretic patterns. When the dissociated virions of two of the mutants were analyzed by the isoelectric focusing technique in a urea gradient, the capsid protein peaks were found to differ significantly in their isoelectric points. The capsid protein of the small-plaque mutant had an isoelectric point of pH 6.51 as compared with pH 6.28 for the large-plaque strain. Isoelectric focusing of the isolated capsid protein revealed three components, a single major subunit and two minor forms. The coat proteins of two of the mutants, small-plaque and minute-plaque strains, were indistinguishable by this technique. The capsid protein peaks obtained by isoelectric focusing were further analyzed by polyacryalmide gel electrophoresis.


Cytomegalovirus replication in cells pretreated with 5-iodo-2'-deoxyuridine. The replication of human cytomegalovirus (CMV) in cells pretreated with 5-iodo-2'-deoxyuridine (IUdR) was studied. Pretreatment of cells with IUdR enhanced several parameters of virus replication. Virus grown in drug-treated cells exhibited a shorter eclipse period and the cells produced more infectious virus sooner than did untreated cells. There was an approximate fivefold increase in virus yield per cell in the drug-treated samples when compared to control cultures. The time required for plaque development was shortened by 6 days in drug-treated cultures. Pretreatment of cells with IUdR also increased plaquing efficiency of the virus by approximately 10-fold. The enhancement of virus replication by IUdR was further demonstrated by varying the multiplicity of infection. In a 7-day period there was a 100-fold increase in sensitivity of the cultures for virus detection when the cells had been previously exposed to IUdR. The data presented indicate the possibility that IUdR interferes with the production of a cellular product inhibitory for CMV replication.


Suppression of amber mutations of bacteriophage T4 gene 43 (DNA polymerase) by translational ambiguity. The growth properties of twelve different amber (am) mutants of bacteriophage T4 gene 43 (DNA polymerase) were examined by using nonpermissive (su(-)) as well as permissive (su(+)) Escherichia coli hosts. It was found that most of these mutants were measurably suppressed in su(-) hosts by translational ambiguity (misreading of codons during protein synthesis). The ability of these mutants to grow in response to this form of weak suppression probably means that the T4 gene 43 DNA polymerase can be effective in supporting productive DNA replication when it is supplied in small amounts. By similar criteria, studies with other phage mutants suggested that the products of T4 genes 62 (uncharacterized), 44 (uncharacterized), 42 (dCMP-hydroxymethylase), and 56 (dCTPase) are also effective in small amounts. Some T4 gene products, such as the product of gene 41 (uncharacterized), seem to be partially dispensable for phage growth since am mutants of such genes do propagate, although weakly, in streptomycin-resistant su(-) hosts which appear to have lost the capacity to suppress am mutations by ambiguity.


Nonproductive infection of guinea pig cells with human cytomegalovirus. Human cytomegalovirus was capable of adsorbing to and penetrating guinea pig cells, but was unable to replicate new virus. Cultures infected with virus inoculum of high titer showed a cytopathic effect (CPE) characterized by cell rounding. This CPE depended upon the presence of infectious virus, and its extent was directly related to the multiplicity of infection. Staining by indirect immunofluorescence by using human convalescent sera was positive as early as 4 h postinfection. Maximal fluorescence was observed 24 h postinfection when 50% of the cells contained fluorescent antigens both in nuclei and cytoplasm. No evidence for viral replication was found, and no defective particles were detected by electron microscopy. Treatment with actinomycin D or with cycloheximide strongly inhibited both the fluorescent antigens and the CPE, whereas 5-fluorodeoxyuridine and bromodeoxyuridine were ineffective.


Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. In high-multiplicity infection of human fibroblasts, human cytomegalovirus of WI-38 human diploid cells produced early cell rounding 6 to 24 h after inoculation. This early cell rounding was caused only by inoculation with infectious virions. Inhibitors of protein synthesis, but not DNA inhibitors, prevented this cytopathic effect. Apparently, a new protein is synthesized in infected fibroblasts from about 2 h postinoculation. Infectivity of cell-associated and supernatant infectious virus reached maximal levels at 5 to 7 and 10 days postinoculation, respectively. Synthesis of DNA, infectious virus, complement-fixing antigen, and precipitin antigen all began between 24 and 48 h, with the bulk of synthesis occurring 48 to 96 h postinoculation.


Intrauterine infection with herpes simplex virus. Resultant congenital malformations.


Benign intrathoracic lesions mimicking recurrent cancer.


Methodology and management of simian toxicology studies with emphasis on special technics.


Hematologic and serum electrolyte values of the C57BL-6J male mouse in maturity and senescence.


Breeding the African green monkey, Cercopithecus aethiops, in a laboratory environment.


Pathogenic properties of Molineus torulosus in capuchin monkeys, Cebus apella.


Cataract development in streptozotocin-diabetic monkeys (Macaca nemestrina).


Comparison of old tuberculin and purified protein derivative in Macaca mulatta.


Response of mice to Corynebacterium kutscheri on footpad injection.


Some observations on the circadian rhythm of Choloepus hoffmanni, the two-toed sloth.


Amino acid levels in the plasma of fasting macaques (Macaca cyclopis, M. fascicularis, and M. mulatta).


Production of hyperimmune serum with mature rabbits.


Isolation and in vitro characterization of a herpesvirus from field mouse (Microtus pennsylvanicus).


Effect of anesthetic agents on exercised, atropinized rats.


Fibrosarcoma associated with Cysticercus fasciolaris.


Some characteristics of strain SJL-JDg mice.


Differential diagnosis of an obscure bone disease in an infant rhesus monkey.


A method for preventing oral ingestion of topically applied materials.


Meckel's diverticulum in a rat.

Meckel's diverticulum in a rat.


A mouse bleeding technic yielding consistent volume with minimal hemolysis.


Basal-cell tumor in a Virginia opossum (Didelphis marsupialis).


Spontaneous lesions seen at necropsy in 7 owl monkeys (Aotus trivirgatus).


Nonsegregated acute psychiatric admissions to general hospitals--continuity of care within the community hospital.


Proteins of human cortical neurons during development.


Concentrations of human chorionic gonadotropin, progesterone, and unconjugated and total estriol in umbilical artery and vein plasma at term.


The assay of ACTH in plasma.

The assay of ACTH in plasma.


Fibrous histiocytoma of the paranasal sinuses. Report of a case.


Synovial sarcoma of the head and neck.

Synovial sarcoma of the head and neck.


The influence of oral contraceptive steroids on serum lipids. The effects of oral contraceptives on serum lipids were investigated in humans and monkeys at the Temple University Health Sciences Center.  The compositions of the pills were: 1) 2.0 mg chlormadinone acetate, .08 mg mestranol; 2) 10.0 mg medroxyprogesterone acetate, .05 mg ethinyl estradiol; 3) 1.0 mg ethynodiol diacetate, .1 mg mestranol; 4) 1.0 mg norethindrone acetate, .05 mg  ethinyl estradiol, and 5) .5 mg norgestrel, .05 mg ethinyl estradiol.  238 immediately puerperal black women, healthy and aged 20-30, were divided into 6 groups for study purposes; each of 5 groups used a separate oral therapy, while the sixth group (control) was fitted with a plastic intrauterine device.  75 women were still participating in the study at 54 weeks.  All of the drugs significantly increased the concentrations of serum cholesterol, serum triglycerides, total phospholipids, and fatty acids.  The influence of the drugs on lipoproteins was variable.  The higher its progestogen/estrogen ratio, the greater influence a drug had on lipid concentrations.  Some of the same effects were seen in 14 mature female squirrel monkeys (Cebus albifrons), which were studied in a similar manner for about 30 cycles; however, only drugs 1, 2, and 3 were used in this part of the study.  Clinical observations of the patients in this study, showed no relationship between serum phosphatides participating in the coagulation mechanism and the production of thromboembolic phenomena.  The researchers found no justification for the delay of oral contraceptive prescription after childbirth.


Solomon A. Berson and Rosalyn S. Yalow: a scientific appreciation.


Possible clues in the continuing search for the subcellular basis of thyroid hormone action.


Bilateral glomus tumors of the temporal bone. Report of a case.


Radioimmunoassay of human plasma ACTH and the pathogenesis of Cushing's disease.


Lipid composition of selected strains of Yersinia pestis and Yersinia pseudotuberculosis.


Berson and Yalow's radioimmunoassay for parathyroid hormone (PTH): a clinical progress report.


Observations on the control and release of growth hormone in plasma.


The enzymic synthesis of disialoganglioside: rat brain cytidine-5'-monophospho-N-acetylneuraminic acid: monosialoganglioside (G M1 ) sialyltransferase.


Secondary hyperparathyroidism.

Secondary hyperparathyroidism.


The stimulation of bovine epididymal sperm metabolism by cyclic nucleotide phosphodiesterase inhibitors.


Gonadotropin receptor of a mouse luteoma: interactions with luteinizing hormone (LH) and its   and   subunits. A radioligand-receptor system for luteinizing hormone (LH), USING transplantable mouse luteoma, was used to investigate the interactions of LH, other peptide hormones, and LH subunits.  Since tumor size decreased as did production of androgenic hormones following hypophysectomy, the luteoma is believed to have been dependent on pituitary tropic hormones; posthypophysectomy histologic changes supported this conclusion.  An homogenate was prepared from 1-4 gm luteomas, which had been borne by mice for 4-10 months.  Ovine LH, bovine LH, and human chorionic gonadotrophin reduced the binding of iodine-125 human luteinizing hormone (125-I-hLH).  Growth hormone, adrenocorticotrophic hormone, and prolactin had no capacity to interfere with binding of 125-I-hLH.  Though follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH) reduced the binding somewhat, the reductions were consistent with the known presence of contaminating amounts of LH in the FSH and TSH.  The accumulated results of a number of experiments suggest that binding to the luteoma LH receptor requires a particular polypeptide structural conformation, one found in the native hormone but found in neither alpha nor beta subunit alone.


Lipids in tissues of the eye. 8. Sphingomyelinase of the lens.


The effects of cyclic nucleotide phosphodiesterase inhibitors on ejaculated porcine spermatozoan metabolism.


Cell-mediated immunity and antibodies to Herpesvirus hominis type 1 in oral leukoplakia and carcinoma. Cell-mediated and humoral immune responses to Herpesvirus hominis type 1 (HVH1) and Candida albicans were studied in patients with leukoplakia, showing a histological spectrum of changes from epithelial keratosis to acanthosis and atypia, and in patients with carcinoma. The results were ranked according to increasing values of stimulation indices of lymphocyte transformation to HVH1, and the corresponding macrophage migration inhibition indices, and complement fixing antibody titres of each patient were correlated. This revealed that most patients with epithelial atypia were clustered to that end of the spectrum which had the highest stimulation and migration indices to HVH1; this relationship was not evident with C. albicans. In patients with keratosis and acanthosis there was a significant lack of correlation between lymphocyte transformation and migration inhibition to both HVH1 and C. albicans. In carcinoma the indices of lymphocyte transformation and migration inhibition to HVH1 and C. albicans were depressed. Furthermore, a significant negative correlation was found between lymphocyte transformation and migration inhibition to HVH1, unlike the positive correlation in control subjects. Complement fixing antibodies to HVH1, HVH2, cytomegalovirus and adenovirus, and fluorescent antibodies to C. albicans failed to show a significant change in titre in any one group of subjects tested. The results suggest a cell-mediated immune defect in leukoplakia, with a dissociation between lymphocyte transformation and macrophage migration inhibition to HVH1 and C. albicans in cases of keratosis or acanthosis. A specific increase in cell-mediated immunity to HVH1 in leukoplakia with epithelial atypia and the sequential changes argue in favour of a possible participation of HVH1 in carcinomatous transformation of some leukoplakias.


Tomography of the cavernous sinuses.

Tomography of the cavernous sinuses.


The effects of arterial blood pressure on the regional cerebral blood volume by X-ray fluorescence.


Excretion of alpha-foetoprotein in the urine of pregnant rats and hepatoma-bearing animals. Urine of normal rats, pregnant animals and animals bearing chemically induced hepatoma was tested with antisera to foetoproteins by the double immunodiffusion technique. Antigens were not detected in the urine of normal rats. Alpha-foetoprotein was demonstrated in the urine of pregnant rats and hepatomabearing animals.


Thalamic regulation of synaptic transmission in the ventrolateral nucleus during natural sleep and waking.


Two negative dorsal root potentials evoke a positive dorsal root potential.


The neural pathways relaying reflex inhibition from semicircular canals to extraocular muscles of rabbits.


Influence of the locus coeruleus on transmission in the spinal trigeminal nucleus neurons.


Acetylcholine and slow synaptic inhibition in frog sympathetic ganglion cells.


Slow synaptic inhibition: evidence for synaptic inactivation of sodium conductance in sympathetic ganglion cells.


Angiotensin as a possible intrarenal hormone in isolated dog kidneys.


Metastatic ovarian cancer.

Metastatic ovarian cancer.


Purification of a messenger-specific initiation factor from ascites-cell supernatant.


Reduced Bohr effect in NO-ligated chironomus haemoglobin.


Interactions of substrates with a purified 4-methoxybenzoate monooxygenase system (O-demethylating) from Pseudomonas putida.


Structure of protocollagen proline hydroxylase from chick embryos.


Biosynthesis of cartilage procollagen.

Biosynthesis of cartilage procollagen.


IUPAC-IUB commission on biochemical nomenclature (CBN). Nomenclature of iron-sulfur proteins. 1973 recommendations.


Electron-transport chains of Escherichia coli. Reconstitution of respiration in a 5-aminolaevulinic acid-requiring mutant.


86 Rb transport, water and cation concentrations in lenses exposed to polymyxin B sulfate.


Kinetics of the interaction between pig-plasma benzylamine oxidase and substrate.


EMS-induced mitotic recombination in a diploid, heterozygous for two complementing ade2 alleles of Saccharomyces cerevisiae.


Isozymes of experimentally induced primary sarcomas in the rat.


Lymphocyte and antibody cytotoxicity to tumor cells measured by a micro- 51 chromium release assay.


Periodate induced lymphocyte transformation. I. Inhibition by sulfhydryl reducing agents and sugars.


Induction of systemic delayed hypersensitivity during experimental viral infection of the respiratory tract with a myxovirus or paramyxovirus.


Effects of pyran copolymer on leukemogenesis in immunosuppressed AKR mice.


Tumors of minor salivary glands. A study of 23 cases.


Insulinoma in an eighty-year old.

Insulinoma in an eighty-year old.


Gastrointestinal bleeding and hepatoma. Diagnostic value of celiac angiography.


An attempt to evaluate the influence of neighboring amino acids (n-1) and (n+1) on the backbone conformation of amino acid (n) in proteins. Use in predicting the three-dimensional structure of the polypeptide backbone of other proteins.


Spreading depression, sustained potential shifts, and metabolic activity of cerebral cortex of cats.


Bilateral nephroblastoma.

Bilateral nephroblastoma.


Fibrous histiocytoma of the scrotum in an infant.


Occurrence of (+)-erythro-2,3-dihydroxyhexacosanoic acid in cerebrin from the yeast Saccharomyces cerevisiae.


Diagnosis of mammary cancer.

Diagnosis of mammary cancer.


Sarcomas of the breast.

Sarcomas of the breast.


Pathology of mammary carcinoma.

Pathology of mammary carcinoma.


Segregation kinetics of colicinogenic factor col E1 from a bacterial population temperature sensitive for DNA polymerase I.


Kinetics of phosphorylation and dephosphorylation of testis histones and their possible role in determining chromosomal structure.


Stimulation of cAMP synthesis by glucose in a mutant of E. coli B-r.


Psychiatric complications of everyday drugs.


Health hazards of silicious material.

Health hazards of silicious material.


Metabolic alterations after chronic exposure to  -chlordane.


Stereophotogrammetric techniques for measurements of the eye ground. An analysis of the correlation and variation of parameters measured from the optic cup and disc in 115 subjects.


Hydrosmotic effect of angiotensin II: isolated toad skin.


Increased cardiovascular reactivity after bilateral nephrectomy in the rat.


The action of trypan blue on intraparticulate hydrolysis in liver phagolysosomes and on a lysosomal enzyme. The linking of the dye to proteins.


Age factor and the pattern of change in craniofacial structures.


Growth standards for the tibia and radius in children aged one month through eighteen years.


Norms of size and annual increments of six anatomical measures of the cranium in boys and girls from four to fifteen years of age.


The dating of Lantian man and his significance for analyzing trends in human evolution.


Osteoporotic bone loss in two prehistoric Indian populations.


Parathyroid hormone receptor in bovine kidney cortex plasma membranes.


A function of synovial membrane of normal and diseased humans in relation to the movement of small-molecular-weight ions.


Uptake of vitamin B 12  by phytohaemagglutinin-transformed lymphocytes.


Stimulation of H-3-leucine incorporation into the proinsulin and insulin fraction of isolated pancreatic mouse islets in the presence of glucagon, theophylline and cyclic AMP.


Pancreatic islet transplantation in the rat.


Cross relationships among 37 rhinoviruses demonstrated by virus neutralization with potent monotypic rabbit antisera. Antisera produced in rabbits to 37 rhinovirus (RV) types have been examined for antibody to the 36 corresponding heterologous types. Reciprocal neutralization was noted between RV types 2 and 49 and RV types 13 and 41. Five additional monotypic rhinoviral rabbit antisera neutralized one heterologous rhinovirus. Neutralizing antibody titers against heterologous RV serotypes were similar to those shown in the RV 1A, 1B and RV 9, 32 reciprocal cross-reactions, and would not be expected to result in false identification of serotypes. Comparison of neutralizing antibody titers and neutralization rate constants in serial serum specimens of immunized rabbits showed that antibody response to the immunizing RV type and the cross-reacting type followed a similar time pattern.


Guinea pig herpes-like virus infection. I. Antibody response and virus persistence in guinea pigs after experimental infection. Guinea pigs, experimentally infected with guinea pig herpes-like virus produced antibodies which were detectable by indirect hemagglutination (IHA), complement-fixation (CF), and neutralization tests. The IHA test appeared to be a more sensitive method than the CF or neutralization test for determining antibody response in guinea pigs immediately after infection, but the IHA method was not suitable for the detection of antibody in animals receiving small doses of virus. Antibody titers obtained by CF tests were generally higher than those obtained by the neutralization test, and they followed the same time course when individual animals were studied serially. Intracardiac inoculation produced the best antibody response in guinea pigs when compared with other routes of infection. Guinea pigs infected by the intraperitoneal, intranasal, or oral route showed rising antibody titers but the levels were low. Infectious virus was isolated from and persisted in all inoculated animals in the presence of antibody regardless of the route of inoculation. Recovery of infectious virus required cultivation or cocultivation of tissue cells containing virus. The administration of antilymphocyte sera delayed the appearance of IHA antibody but had no effect on antibodies determined by the CF- and neutralization tests.


Interaction of lymphoid and nonlymphoid cells with the lymphocytosis-promoting factor of Bordetella pertussis. The activity of soluble lymphocytosis-promoting factor (LPF) from Bordetella pertussis decreased after brief in vitro incubation with lymphoid cells or erythrocytes. The LPF activity was found to be associated with the cells used, and injection of the cells produced a leukocytosis and lymphocytosis which completely accounted for the loss of soluble activity. Attachment of LPF to cells was found to be reversible in vitro. It is suggested that reversible binding occurs in vivo.


Guinea pig herpes-like virus infections. II. Antibody response and virus persistence in mice and rabbits. Mice inoculated with guinea pig herpes-like virus produced minimal, if any, antibody response, and no virus was isolated from the inoculated animals 64 days after administration. The antibody response in rabbits inoculated with the same virus was prompt and reached a high level within 14 to 29 days regardless of the site of inoculation. Long-term persistence of viremia was observed only in intravenously inoculated rabbits; a brief period of viremia was observed in intraperitoneally inoculated rabbits, but no viremia was obtained in rabbits inoculated by the subcutaneous route. Maternal antibody was transferred readily to offspring; however, transference of infectious virus from mother to offspring was demonstrable in only one of 75 fetuses tested.


Naloxone and the narcotic abuser: a low-dose maintenance program.


A calcium-sensitive lanthanum inhibition of amoeboid movement.


Colony formation by chicken hematopoietic cells and virus-induced myeloblasts.


Immune status of children one to four years of age as determined by history and antibody measurement.


Conference and workshop on cellular immune reactions to human tumor-associated antigens. Introduction.


Mechanisms of lithium action.

Mechanisms of lithium action.


Neurilemmoma of the facial nerve.

Neurilemmoma of the facial nerve.


Effectiveness of extended aftercare in the treatment of alcoholism.


Subgroup classification of feline leukemia and sarcoma viruses by viral interference and neutralization tests.


Analysis on the reproducing and cell-transforming capacities of a temperature sensitive mutant (ts 334) of avian sarcoma virus B77.


Immunological characterization of a low molecular weight polypeptide of murine leukemia virus.


RNA species obtained from clonal lines of avian sarcoma and from avian leukosis virus.


Poly(rI) more important than poly(rC) in the interferon induction process by poly(rI)-poly(rC).


Complementation of transforming functions by temperature-sensitive mutants of avian sarcoma virus.


The synthesis of complementary ribonucleic acid during infection with LSc poliovirus.


Characterisation of temperature-sensitive mutants of herpes simplex virus type 2. Growth and DNA synthesis.


A structural model for picornaviruses as suggested from an analysis of urea-degraded virions and procapsids of coxsackievirus B3.


Prophage deletion mapping of bacteriophage Mu-1.


Cytomegalovirus infection: a seroepidemiologic study of a recruit population.


Epidemiologic and clinical observations in six outbreaks of viral disease in a kibbutz, 1968-1971.


Variant angina associated with angiographically demonstrated coronary artery spasm and REM sleep.


Ultrastructural study of two gonadotrophs in rats bearing an adrenocortical carcinoma (Snell 494). Two morphologically distinct gonadotrophs have been identified in the anterior pituitary of rats bearing the Snell 494 tumor. Type 1 gonadotrophs first showed ultrastructural alterations at 4 weeks after implantation of the tumor. Numerous large 300 to 600 mmu granules appeared in type 1 cells. The opacity of the granules was variable, and they sometimes contained non-membrane-bounded spherical inclusions. These granules were dispersed among more electron-opaque, 200-mmu granules usually seen in gonadotrophs. A third granule approximately 500 to 600 mmu in diameter was apparently a lysosome-derived structure. Type 2 gonadotrophs first showed changes after 4 weeks, but the alterations were especially prominent by 8 to 10 weeks. No large granules characteristic of type 1 cells were seen in type 2 cells. By 8 to 10 weeks, very few 200-mmu granules were observed in the cytoplasm of type 2 cells. The endoplasmic reticulum was dilated in most type 2 cells; frequently, the cisternae were dilated considerably, forming large lake-like areas. Hypertrophic rough endoplasmic reticulum and the presence of intracisternal granules within cisternae of the rough reticulum suggest that type 2 cells were stimulated by the tumor. Changes in gonadotrophs were undoubtedly produced by the large amount of corticosterone secreted by the tumor. The corticosterone secreted by the tumor. The corticosterone may well act directly on the hypothalamo-hypophyseal axis or it could act indirectly on the ovary; ovarian steroids would then exert a feedback effect. The ovary was atrophic and did not contain maturing Graafian follicles or corpora lutea. In somatotrophs, the number of secretory granules increased, probably reflecting suppression of hormonal release by corticosterone.


Virus cross-infection in paediatric wards. In a survey of virus cross-infection in paediatric wards there were 15 cross-infections due to respiratory syncytial (R.S.) virus and 16 due to influenza A, both during a four-month period, and 19 due to parainfluenza viruses over two years. The illnesses produced by these infections acquired in hospital ranged from a slight cold to severe pneumonia: in 17 of the 50 cases the illness involved the lower respiratory tract. A measure of cross-infection frequency in the form of a "cross-infection rate" has been devised, and it is suggested that this can be used to assess the influence of factors such as ward design and admission policy on the frequency of cross-infection.


Differences between cerebellar mossy and climbing fibre responses to natural stimulation of forelimb muscle proprioceptors.


Visual cortical units associated with phasic activity in REM sleep and wakefulness.


Herpes simplex virus type 2 in acute aseptic meningitis.


Glial metabolism: alteration by potassium levels comparable to those during neural activity.


Enhanced striatal acetylcholine release by chlorpromazine and its reversal by apomorphine.


Cholinergic neurons: effect of acute septal lesion on acetylcholine and choline content of rat hippocampus.


Propagation of afterdischarges elicited in thin brain sections in artificial media.


Olivo-cochlear inhibition during physostigmine-induced activity in the pontine reticular formation in the decerebrate cat.


Relationships between pre- and postsynaptic effects of A and C fiber inputs to dorsal horn of M. mulatta.


Effects of neuromuscular activity on choline acetyltransferase and acetylcholinesterase.


In vivo destruction of tumor cells by an indirect cell-mediated immune mechanism.


Biological testing of leukaemic lesions of ICRC mice for possible viral activity.


Delayed effects of a single treatment of hydroxyurea to newborn mice.


Comparison of in vitro antiamoebic activity of MK 910 with metronidazole, intestopan and enterovioform.


Antibodies to phospholipids in human tuberculous sera.


Basement membrane antigens combining with antisera to human glomerular asement membrane and lymphocytes.


Constituents of Cannabis sativa L. 3. Clear and discrete separation of cannabidiol and cannabichromene.


Neoplasm of mixed mesenchymal and neuroepithelial origin: liposarcomatous meningioma combined with gliomas. A case is reported of an intracranial neoplasm of mixed mesenchymal and neuroepithelial (glial) origin occurring in the parieto-parasagittal region of the brain of a 19 year old man. A meningioma with liposarcomatous and psammomatous components comprised the mesenchymal part of the combined tumour. The neuroepithelial portion was composed of mixed ependymoma and astrocytoma. The combined neoplasm had the gross appearances and several microscopic features of giant-celled glioblastoma, giant cell sarcoma, and monstrocellular sarcoma. The findings suggest that these tumours have a mixed mesenchymal and neuroepithelial origin.


The initiation of a "metabolic window" in the surface of host erythrocytes by Plasmodium berghei NYU-2.


False-positive monospot or rubella and mononucleosis.


Regulation of cholesterol biosynthesis in rat liver after the administration of N-2-fluorenylacetamide.


Epithelial tumours of the salivary glands.


Brief exposure of bacteria to topical antibiotics.


Tissue response to ceramic foam dust following intratracheal and intraperitoneal administration.


Effects of drugs or hepatotoxins on the relation between drug-metabolizing activity and phospholipids in hepatic microsomes during choline deficiency.


Effects of prostaglandins E 1 , A 1 , and F 2   on renal handling of salt and water.


Micropharmacology of vertebrate neuromuscular transmission.


Control of gluconeogenesis and glycogenolysis in perfused livers of normal mice.


Drug research and human sleep.

Drug research and human sleep.


Radioimmunoassay.

Radioimmunoassay.


Sympathetic mechanisms in blood vessels: nerve and muscle relationships.


Renal pharmacology, with special emphasis on aldosterone and angiotensin.


Endocrine disorders of growth in stature.


Glucagon infusion following phosphodiesterase inhibition: an experimental study of the hemodynamic effects.


Photodynamic inactivation in the treatment of experimental herpes simplex keratitis.


Epidemic keratoconjuctivitis.

Epidemic keratoconjuctivitis.


Stimulation of corticotropin release.

Stimulation of corticotropin release.


Enzyme activity of antigen-stimulated macrophages.


Mechanism of hypertriglyceridaemia associated with contraceptive steroids.


Effect of collagenase on human growth hormone and ACTH.


A micro-method for the measurement of pharmacologically induced changes in purine derivatives.


The prevalence of hyperlipidemia in a natural community.


Möbius syndrome with Poland's anomaly.

Möbius syndrome with Poland's anomaly.


A physico-chemical sub-grouping of the mammalian picornaviruses.


Effects of cortisol on evoked potentials and recovery cycles in the rat hypothalamus.


Malakoplakia: an electron-microscope and chemical study.


Pituitary-adrenal influences on behavior: responses to test situation with or without electric footshock.


The climacteric.

The climacteric.


Cell culture propagated fowl pox vaccine.


A comparative ultrastructural study of the effects of chlorphentermine and triparanol in rat lung and adrenal gland.


Monkeypox and its relevance to smallpox eradication.


Virilizing adrenal adenoma.

Virilizing adrenal adenoma.


Attempt to influence viral mouse leukaemia by simultaneous infection with influenza A1 virus.


The effect of parachlorophenylalanine on the sleep of a methadone addict.


Actinomycin D for Wilms's tumour.

Actinomycin D for Wilms's tumour.


'Psychopathia Sexualis'.

'Psychopathia Sexualis'.


Temperature-induced phase changes in membranes of heart: a contrast between the thermal response of poikilotherms and homeotherms.


Using inpatient staff for aftercare of severely disturbed chronic patients.


Malignant hepatoma.

Malignant hepatoma.


Microbiochemical analysis of the arterial wall. I. Phosphofructokinase.


'Sclerosing haemangioma' of the lung: an alternative view of its development.


Studies of the mechanisms of carbohydrate-induced lipaemia in normal man.


Collagen synthesis in thoracic aortas of rabbits with epinephrine-thyroxine induced arteriosclerosis.


Spin-labeled analogs of local anesthetics.


Synthesis of 5-trifluoromethyl-2'-deoxyuridine 5'-phosphate and 5-trifluoromethyl-2'-deoxyuridine 5'-triphosphate.


DNA synthesis in nucleotide-permeable Escherichia coli cells. VII. Conversion of phi chi-174 DNA to its replicative form.


Functional overlap between "chylomicra" and "very low density lipoproteins" of human plasma during alimentary lipaemia.


Reciprocal effects of change of subunit structure on ligand equilibria of haemoglobin valency hybrids. Attempted correlation with electron paramagnetic resonance spectra.


An evaluation of cellulose acetate electrophoresis for the determination of human plasma lipoprotein patterns.


Electron paramagnetic studies of nitric oxide haemoglobin derivatives: isolated subunits and nitric oxide hybrids.


Interaction between lambda or phi80 prophage induction and gal operon expression.


"Polymyositis" with involvement of facial and distal musculature. One form of the fascioscapulohumeral syndrome?


Anxiety as a manifestation of associated drives and events in a female schizophrenic patient.


Unilateral ureteral obstruction. A caue of reversible high renin content hypertension.


Uptake and effect of 17 -estradiol on pineal hydroxy-indole-O-methyl transferase (HIOMT) activity.


Mental health nursing consultations: some distinctions.


Thyroidal K + -activated p-nitrophenylphosphatase activity.


Speaking out on: second career adds to nurse supply.


The yellow brick road: a legitimate educational pursuit.


Two distinct antigenic components in an Epstein-Barr virus-related early product induced by halogenated pyrimidines in non-producing human lymphoblastoid cells.


Non-repetitive DNA transcription in mouse cells grown in tissue culture.


Development of the co-therapy relationship.


Polyadenylate synthesis by extracts from L cells infected with vesicular stomatitis virus.


Quantitative determination of drugs by means of densitometry of thin-layer chromatograms. 3. Determination of vitamins D 2  and D 3  in oil and aqueous injection solutions in the presence of decomposition products.


Delivery of mental health services to children of "resistive" parents.


Mental illness and the state (Roche report).


Psychiatric drugs and trends: long acting tranquilizer.


Abrasive blasting protective practices study--preliminary survey results.


Leprosy--an alternative viewpoint.

Leprosy--an alternative viewpoint.


Alexia without agraphia or hemianopsia. Anatomical analysis of an autopsied case.


The effect of pyrimidine compounds on the potentiation of adenosine inhibition of aggregation, on adenosine phosphorylation and phosphodiesterase activity of blood platelets.


Hyperlipaemic neuropathy, Experimental study.


Small group method on a general hospital nursing unit.


Leisure time for the elderly.

Leisure time for the elderly.


Genetic influence on the renin-angiotensin system: low renin activities in hypertension-prone rats.


Intrarenal role of angiotensin II. Homeostatic regulation of renal blood flow in the dog.


Variations in adrenergic innervation and contractile responses of the rabbit saphenous artery.


The sudden infant death syndrome.

The sudden infant death syndrome.


The action and interaction of diphenhydramine (Benadryl) hydrochloride at the neuromuscular junction.


Studies about unconjugated androstenediol in human peripheral plasma.


Measurement of plasma renin substrate by radioimmunoassay of angiotensin. I. Concentration in syndromes associated with steroid excess. Plasma renin substrate (PRS) and plasma renin activity (PRA) were measured by radioimmunoassay of generated angiotension I in normal subjects and patients with steroid excess syndromes.  In subjects with Cushing's syndrome, glucocorticosteroid therapy and oral contraceptive agents or estrogens, significant elevations of plasma renin substrate was observed.  Only in subjects with primary aldosteronism was the plasma renin activity significantly supressed.  The significance of elevations of PRS was assessed by determining the Km of the plasma renin-substrate reaction at physiologic pH.  The apparent Km for the reaction was 1000 ng Angiotension I in PRS units.  Correction of measured PRA to a standardized PRA was achieved by calculation of the reaction rate that would occur at the mean normal PRS concentration of 1100 ng/ml.  Results indicate that elevations of PRS which occur in Cushing's syndrome or with administration of glucocorticoids, oral contraceptive agents, or estrogens are sufficient to significantly elevate the renin substrate reaction in plasma.  In contrast, glucocorticoid excess appears not to suppress plasma renin activity even when reaction rates are corrected for increased PRS.


Effect of hypopituitarism and ACTH on the urinary excretion of norepinephrine in man.


Adenosine 3',5'-monophosphate in amniotic fluid from normal pregnancy.


Effect of hydrocortisone and corticotropin on glucose-induced insulin and proinsulin secretion in man.


Nocturnal patterns of serum gonadotropins during the menstrual cycle.


Dexamethasone-suppressible hyperaldosteronism.


Inference of refractory period, temporal summation, and adaptation from behavior in chronic implants: midbrain pain systems.


Behavioral measurements of the neural poststimulation excitability cycle in the lateral hypothalamic eating system of the rat.


The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2.


Further evidence for two loci which control susceptibility of fowl to RSV(RAV-O).


2,3-Erythro-dihydroxyhexacosanoic acid and homologs: isolation from yeast cerebrin phosphate and determination of their structures. Homologs of methyl esters of very polar fatty acids were obtained by methanolysis of cerebrin phosphate isolated from baker's yeast. The major ester component was isolated by preparative gas-liquid chromatography and was found to be 2,3-dihydroxyhexacosanoic acid as deduced from the mass spectra of its trimethylsilyl ether and isopropylidene derivative, reaction with periodate, and comparison of its chromatographic behavior with that of synthetic erythro- and threo-dihydroxy acids. Its infrared spectrum supported the above conclusions. From their retention times by gas-liquid chromatography, the homologs were found to be saturated, unbranched 2,3-dihydroxy fatty acids with 24-27 carbon atoms. The synthesis of the new fatty acids, erythro- and threo-2,3-dihydroxyhexacosanoic acids, is also reported. A method for separating trans-2-hexacosenoic acid, a key intermediate of the above synthesis, and its isomer, trans-3-hexacosenoic acid, both formed by dehydrobromination of 2-bromohexacosanoic acid, is also described.


Metabolic fate of rat and human lipoprotein apoproteins in the rat. The fate of (125)I-labeled apolipoproteins was studied in vivo in rats that had received intravenous injections of (125)I-labeled rat HDL and (125)I-labeled human HDL, LDL, and VLDL. Plasma decay curves of rat and human HDL were exponential with similar half-lives in the circulation (11-12 hr). After injection, low molecular weight apolipoproteins (apoLP-alanine of human HDL and fraction HS-3 of rat HDL) were found to redistribute to other lipoproteins, predominantly VLDL. Decay curves of individual HDL proteins were constructed after lipoprotein fractionation, delipidation, and polyacrylamide gel electrophoresis. It was found that the half-lives of the different HDL apoproteins were not identical. A major rat HDL protein (52% of total counts) had a circulating half-life (t((1/2))) of 12.5 hr. Two others had a t((1/2)) of 8-9 hr while the t((1/2)) of several others was 11-12 hr. The t((1/2)) of three well-characterized human HDL apoproteins, apoLP-glutamine I, apoLP-glutamine II, and apoLP-alanine, were 13.5, 9.0, and 15.0 hr, respectively. The fate of (125)I-labeled human VLDL and LDL apoproteins in rats was similar to that described previously in humans. After injection of (125)I-labeled human VLDL into rats, apoLP-glutamic acid and apoLP-alanine rapidly transferred to rat HDL and were lost thereafter from the circulation from both VLDL and HDL. The apoLDL moiety of human VLDL moved metabolically to the LDL density range (d = 1.019-1.063) through a lipoprotein of intermediate density (d = 1.006-1.019).


Occurrence of the enzymes effecting the conversion of acetyl CoA to squalene in homogenates of hog aorta. Homogenates and subcellular fractions of the intimamedia of hog aorta have been prepared and examined for the presence of the enzymes catalyzing the conversion of acetyl CoA to squalene. Enzyme activities effecting the conversion of acetyl CoA to 3-hydroxy-3-methylglutarate (HMG); HMG CoA to mevalonic acid; mevalonic acid to 5-phosphomevalonic acid, 5-pyrophosphomevalonic acid, and isopentenyl pyrophosphate; isopentenyl pyrophosphate to farnesyl pyrophosphate; and farnesyl pyrophosphate to squalene have been demonstrated in these homogenates. The overall conversion of mevalonate to squalene has also been demonstrated with recombined fractions of hog aorta homogenates. Data are also presented that suggest that phosphatases present in the crude homogenates act to cleave farnesyl pyrophosphate to farnesol, and phospho- and pyrophosphomevalonate to mevalonate.


Release of transmitters into the perfused third cerebral ventricle.


Evoked transmitter release at the frog neuromuscular junction in very low calcium solutions.


A comparison of the effects of adenosine triphosphate with noradrenaline and with the inhibitory potential of the guinea-pig taenia coli. 1. The response of the smooth muscle of the guinea-pig taenia coli to adenosine triphosphate (ATP) and related substances was compared with that to noradrenaline and dopamine. The effect of these substances on the inhibitory potential was investigated.2. ATP in concentration of 10(-6)-10(-5)M suppressed the spontaneous spike and relaxed the preparation. ATP in a concentration higher than 10(-4)M hyperpolarized the membrane, reduced the membrane resistance and decreased the inhibitory potential.3. Effects of noradrenaline (5 x 10(-6)M) and of dopamine (5 x 10(-4)M) were similar to those of ATP (10(-3)M). However, the effects of catecholamines were reduced in Cl-deficient solution and blocked by phentolamine (3.5 x 10(-6)M), while the effects of ATP were unaffected.4. A high concentration of phentolamine (3.5 x 10(-4)M) almost abolished the inhibitory potential as well as the effects of ATP. The inhibitory potential and the effect of high concentration of ATP (10(-3)M) were not impaired by imidazole (50 mM).5. Adenosine diphosphate, adenosine monophosphate and adenosine produced effects essentially similar to those of ATP. However, their potency was less compared with ATP.6. These electrophysiological observations support the hypothesis that ATP may be the transmitter of the non-adrenergic inhibitory nerves in the taenia coli. No evidence against the hypothesis was obtained.


Advances in lipoprotein research: biochemical and clinical aspects.


Biochemical mechanisms in myelin deficient mutants.


Adenosine release from isolated fat cells and its significance for the effects of hormones on cyclic 3',5'-AMP levels and lipolysis.


Sugar transport in normal and Rous sarcoma virus-transformed chick-embryo fibroblasts. 3-O-methylglucose (3-OMeG) is a nonmetabolizable glucose analog and is, therefore, suitable for transport studies. 3-OMeG and glucose compete for entry into normal and Rous sarcoma virus (RSV)-transformed chick-embryo fibroblasts. Therefore, 3-OMeG can be used to study the transport of glucose in these cells. Chickembryo fibroblasts infected and transformed by RSV take up 3-OMeG at a faster rate than uninfected cells when both cell types are growing at the same rate. The rate of efflux of 3-OMeG also increases after transformation. When the uptake and the efflux reach a steady state, the intracellular concentration of 3-OMeG is equal to the concentration in the medium. This finding indicates that glucose is transported across the plasma membrane by facilitated diffusion. The V(max) of the transport system for 3-OMeG increases after transformation, while the affinity or K(m) of the system remains unchanged. We conclude that viral transformation causes a change in the plasma membrane of the infected cells by increasing either the number of molecules or the mobility of the glucose carrier.


Conversion of neutral to alkaline liver fructose 1,6-bisphosphatase: changes in molecular properties of the enzyme. Removal of the NH(2)-terminal region of fructose 1,6-bisphosphatase from rabbit liver by digestion with subtillisin, or changes in conformation in this region of the protein produced by exposure to low concentrations of urea, result in similar changes in catalytic and allosteric properties of the enzyme. These changes include shift of the pH optimum to more alkaline pH, and loss of sensitivity to inhibition by AMP. The conformation changes are monitored by changes in the fluorescence of the single tryptophan residue, which is located near the NH(2)-terminus. Thus, the tryptophan-containing peptide appears to determine the functional properties of the native enzyme.


Roles of calcium, serum, plasma, and folic acid in the control of proliferation of normal and Rous sarcoma virus-infected chicken fibroblasts. In a culture medium of pH 7.4 and a folic acid concentration of 100 mug/liter that contains 5% heat-inactivated chicken plasma rather than serum, the rate of proliferation of normal chicken fibroblasts is determined by the concentration of calcium. Proliferation, rapid when the calcium concentration is physiological, decreases when the calcium concentration is reduced. At a very low calcium concentration, in this culture medium, normal fibroblasts are maintained without proliferation, whereas those infected with Rous sarcoma virus proliferate rapidly. This proliferative inactivity of normal fibroblasts does not involve contact-inhibition, since the effect is observed at low, as well as higher, culture densities. When a physiological amount of calcium is added to cultures of normal fibroblasts that have been maintained in very low calcium-plasma medium for 3 days, labeled thymidine uptake and protein synthesis are strongly stimulated, and cell division follows. The use of heat-inactivated chicken serum, instead of plasma, in this medium appears to strongly sensitize normal fibroblasts to the mitogenic action of calcium. In a plasma-containing culture medium of physiological calcium concentration and a folate concentration of 5 mug/liter, neither normal nor Rous sarcoma virus-infected fibroblasts proliferate to an appreciable extent. The use of serum, however, instead of plasma results in rapid proliferation of both normal and infected cells, as does increase in the folate concentration of the plasma-containing medium to 100 mug/liter.The fact that while normal fibroblasts are maintained without proliferation in low calcium-plasma medium, Rous sarcoma virus-infected fibroblasts proliferate rapidly, indicates that the effect of calcium is regulatory rather than permissive. These results suggest that the proliferation of normal fibroblasts is initiated by a cellular function involving calcium, and that the autonomous proliferation of the neoplastic fibroblasts results either from increased calcium uptake or from an alteration or a hypass of that function. The results also suggest that serum contains a mitogenic factor(s) not present in plasma, possibly a "wound hormone" for fibroblasts.


Demonstration of a cell-surface antigen associated with murine sarcoma virus by immunoelectron microscopy. Cells transformed by murine sarcoma virus have been examined for the presence of a new virus-associated cell-surface antigen by immunoelectron microscopy. A common antigen has been detected on the surface of nonproductively transformed cells that were induced by two different strains of murine sarcoma virus, Kirsten and Moloney. This antigen shows crossreaction with cell lines transformed by murine sarcoma virus that were produced in two different mammalian species, rats and mice. Further, this antigen is distinct from previously described antigens on the surfaces of cells infected by murine leukemia virus, on the viral envelope, and on the surfaces of spontaneously transformed cell lines or cell lines transformed by x-irradiation.


Nucleation, rapid folding, and globular intrachain regions in proteins. Distinct structural regions have been found in several globular proteins composed of single polypeptide chains. The existence of such regions and the continuity of peptide chain within them, coupled with kinetic arguments, suggests that the early stages of three-dimensional structure formation (nucleation) occur independently in separate parts of these molecules. A nucleus can grow rapidly by adding peptide chain segments that are close to the nucleus in aminoacid sequence. Such a process would generate three-dimensional (native) protein structures that contain separate regions of continuous peptide chain. Possible means of testing this hypothesis are discussed.


Pineal protein kinase: effect of enzymic phosphorylation on actinomycin D binding by, and template activity of, chromatin. The ability of protein kinase from bovinepineal gland to phosphorylate calf-thymus chromatin and thereby to alter the association between chromatin DNA and histones was investigated. Phosphorylation of calf-thymus chromatin by pineal protein kinase results in an apparent decreased binding between the histones and DNA in chromatin, as indicated by (i) an increase in actinomycin D-binding sites after phosphorylation and (ii) an increase in the template capacity of the calf-thymus chromatin after phosphorylation. F(1) histone and F(3) histone are the major histone classes in the chromatin that are phosphorylated by the protein kinase. These results support the hypothesis that pineal protein kinase may function at the transcriptional level.


Chemotaxis by the nematode Caenorhabditis elegans: identification of attractants and analysis of the response by use of mutants. The nematode Caenorhabditis elegans is attracted by at least four classes of attractants: by cyclic nucleotides, cAMP and cGMP; by anions, Cl(-), Br(-), I(-); by cations, Na(+), Li(+), K(+), Mg(+); and by alkaline pH values. The nematode's behavioral response to gradients of these attractants involves orientation and movement up the gradient, accumulation, and then habitutation. Comparison of the tracks of wild-type and mutant animals responding to gradients of attractants indicates that sensory receptors in the head alone mediate the orientation response and that the direction of orientation is determined by the lateral motion of the head. Therefore, the orientation response is a klinotaxis.


Affinity binding of intact fat cells and their ghosts to immobilized insulin. Immobilized insulin, prepared by coupling insulin directly to agarose or through hydrocarbon "connecting arms," was demonstrated to be capable of firmly binding intact adipocytes and their ghosts. Various lines of evidence indicate that the insulin receptor on the plasma membrane, in addition to the insulin coupled to the agarose, was responsible for the observed binding. This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. These findings support the idea that the insulin receptor is the trypsin-sensitive site. By use of the differential buoyant densities of the various cell-bead complexes that resulted from the interaction of adipocytes with insulin-Sepharose, a new procedure was developed to demonstrate and study the binding. These complexes could also be demonstrated by interference contrast microscopy. Binding readily occurred under conditions favorable for insulin stimulation of the cells. By coupling tracer amounts of [(125)I]insulin to Sepharose or insulin-Sepharose, the effects of anti-insulin antisera, free insulin, and other peptide hormones and supplemental factors on the buoyant-density distribution of the complexes could be measured, as well as the effects of other ligands coupled to Sepharose.


Carbohydrate catabolism and the enhancement of uptake of galactose in hamster cells transformed by polyoma virus. Untransformed as well as polyoma virus-transformed hamster cells can be grown equally well on a slow catabolite like galactose as on a rapid catabolite like glucose. The rate of uptake of galactose is greatly enhanced in the transformed cells as compared with untransformed cells, and this enhancement of entry was as markedly expressed in galactose-grown cultures as in glucose-grown cultures. Since the transformed cultures grown in glucose medium consume practically all of their carbohydrate, contrary to the galactose-grown cultures, problems dealing with regulation of transport by substrate concentrations have to be dealt with also. The galactose captured by the cells accumulates initially as galactose, alpha-galactose-1-phosphate, and UDP-galactose. However, after a 24-hr growth on a galactose growth medium, the product accumulated was almost exclusively galactitol. In spite of the enhancement of entry of galactose into the transformed cells, the metabolic pathway becomes stalled even before it has reached the stage of glucose-1-phosphate, largely due to a choke of the enzyme UDP-galactose-4-epimerase (EC 5.1.3.2). Among the sparse amounts of catabolic products generated by the transformed cells from galactose, carbon dioxide (allebeit no D-xylose) and lactic acid were found, both of them in much smaller amounts than seen if glucose is the carbohydrate source. Also, growth of transformed cells on a galactose medium gradually tends to become oriented, a phenomenon that could be called "contact promotion." Subsequent addition of glucose disturbs the oriented growth and interferes with contact promotion.


Cytochalasin B: effect on lysosomal enzyme release from human leukocytes. The morphological and biochemical consequences of treatment of human peripheral blood leukocytes with cytochalasin B were studied. Incubation of human polymorphs with cytochalasin B resulted in nuclear and cytoplasmic spreading, but not in spontaneous release of lysosomal enzymes. Cytochalasin B inhibited particle uptake. Consequently, phagocytic vacuoles were not observed; instead, granule contents were discharged directly into the surrounding medium when cytochalasin B-treated cells were challenged with zymosan particles. Cytochalasin B enhanced the release of lysosomal enzymes from human polymorphonuclear leukocytes whether these encountered zymosan particles or immune complexes on a nonphagocytosable Millipore filter. Cytochalasin B-treated leukocytes thus constitute a model system for quantitative study of lysosome fusion. Augmented enzyme release was blocked by prior treatment of cells with pharmacological doses of agents that influence the accumulation of cyclic nucleotides (cyclic nucleotides themselves, prostaglandin E(1)) or by compounds that interfere with microtubule function (e.g., colchicine, vinblastine). These observations suggest that one action of cytochalasin B on phagocytic cells is to remove the normal constraints to merger of granules, either with each other or with the plasma membrane, and that intact microtubule function is required for translocation of lysosomes.


Minor base composition of "70S-associated" 4S RNA from avian myeloblastosis virus. Using a chemical isotope derivative method, we have determined the minor base composition of the "70S-associated" 4S RNA isolated from avian myeloblastosis virus. The minor base composition of this "70S-associated" 4S RNA is strikingly similar to that of the corresponding "free" 4S RNA of the virion. This minor base content, plus the capacity to esterify amino acids, establishes that both of these virion 4S RNA fractions contain transfer RNA. The percentage of minor bases in virion "70S-associated" 4S RNA is, however, much lower than in "free" 4S virion RNA and in myeloblast 4S RNA. The implication is that the "70S-associated" 4S RNA fraction as isolated herein also contains RNA that is not transfer RNA. This latter RNA may represent either degradation products of high molecular weight RNA or indigenous 4S RNA of undetermined function.


Regulation of phosphorylation of a specific protein in toad-bladder membrane by antidiuretic hormone and cyclic AMP, and its possible relationship to membrane permeability changes. Phosphorylation of a specific protein was decreased in intact toad bladders by exposure to either antidiuretic hormone or monobutyryl cyclic AMP. The decrease in phosphorylation caused by these agents preceded the change in electrical potential difference (an indicator of the rate of sodium ion transport) observed in response to the same compounds. The addition of cyclic AMP to homogenates of toad bladder led to a decrease in phosphorylation of the same, or a similar, protein. In subcellular fractionation studies, the effect of cyclic AMP on the phosphorylation of this protein was observed in those fractions rich in membrane fragments, but not in the nuclear or cell-sap fractions. These and other results are compatible with the possibility that the regulation by vasopressin and cyclic AMP of sodium and/or water transport in toad bladder may be mediated through regulation of the phosphorylation of this specific protein.


A transmissible plasmid controlling camphor oxidation in Pseudomonas putida. Earlier papers demonstrated an extensive genetic exchange among fluorescent Pseudomonads; this one documents for genes specifying enzymes of peripheral dissimilation an extrachromosomal array, segregation, and frequent interstrain transfer. An hypothesis is presented of a general mechanism for the formation and maintenance of metabolic diversity. The example used, the path of oxidative cleavage of the carbocyclic rings of the bicyclic monoterpene D- and L-camphor, terminates in acetate release and isobutyrate chain debranching. By transduction, two gene linkage groups are shown for the reactions before and after isobutyrate. The group for reactions before isobutyrate is plasmid borne, contransferable by conjugation, mitomycin curable, and shows a higher segregation rate from cells that are multiplasmid rather than carrying a single plasmid. The genes that code for isobutyrate and essential anaplerotic and amphibolic metabolism are chromosomal. By conjugation plasmid-borne genes are transferred at a higher frequency than are chromosomal, and are transferred in homologous crosses more frequently than between heterologous species. Most isobutyrate-positive fluorescent pseudomonad strains will accept and express the camphor plasmid.


Acetylcholine receptor and ion conductance modulator sites at the murine neuromuscular junction: evidence from specific toxin reactions. The perhydro derivative of histrionicotoxin reversibly blocks the excitatory ionic transduction system in the synaptic and sarcolemmal membranes of mammalian skeletal muscle cells. The efficacy of perhydrohistrionicotoxin as an antagonist at the post-synaptic membrane is increased by the transient presence of acetylcholine in the endplate of innervated muscles and at extrajunctional receptors in denervated muscles. alpha-Bungarotoxin and [(3)H]monoacetyl-alpha-bungarotoxin block the endplate acetylcholine receptors, each binding to the same extent. The effect of bungarotoxin is partially reversible. These electrophysiological results, together with the effects of perhydrohistrionicotoxin and/or d-tubocurarine on the binding of [(3)H]monoacetyl-alpha-bungarotoxin at endplates of murine diaphragm muscle and on the bungarotoxin-elicited irreversible blockade of neuromuscular transmission, suggest that at least two types of sites participate in the synaptic excitation by acetylcholine. One site, competitively blocked by bungarotoxin and by curare, is presumably the acetylcholine receptor. Binding of bungarotoxin at this site is responsible for an irreversible blockade of neuromuscular transmission. The second site, competitively blocked by bungarotoxin and perhydrohistrionicotoxin, is proposed to be part of the cholinergic ion conductance modulator. Binding of bungarotoxin to this site does not result in an irreversible blockade.


Clinical management of advanced gastrointestinal cancer.


Venoms and enzymes: effects of permeability of isolated single electroplax.


Biological activities contaminating preparations of phospholipase C ( -toxin) from Clostridium perfringens.


Isolation of Gymnodinium breve toxin from Florida red tide water.


Colchicine in avian sodium urate and calcium pyrophosphate microcrystal arthritis.


Application of the technique os isoelectric focusing to the study of human serum lipoproteins and their apoproteins.


Activity of glucoso-6-phosphage gluconeogenesis and dehydrogenase enzymes in fertilized and unfertilized loach ova.


Proliferation of vesicular stomatitis virus in leucocytes from rheumatoid patients.


Clinical and laboratory aspects of raised virus antibody titres in systemic lupus erythematosus.


Effect of some adrenal steroid hormones on skin fibroblast replication in vitro.


2',3'-Cyclic nucleotide 3'-phosphohydrolase in the developing human brain.


Direct stimulant effect of cyclic AMP and dibutryl cAMP on glycogenolysis by hepatocytes in histiotypic culture.


Human erythrocyte phosphoribosylpyrophosphate synthetase mutationally altered in regulatory properties.


Activation of endogenous C-type on oncornavirus by 5-bromodeoxyuridine in cultured embryonic rat fibroblasts.


Comment on Wei's theory of synaptic conduction.


Preganglionic sympathetic activity and baroreceptor responses during hypothermia.


Postsynaptic potentials in the mesencephalic and pontomedullar reticular regions underlying descending limbic influences.


Antagonistic effects of semicarbazide and pyridoxine on cuneate presynaptic inhibition.


Regional differences within the caudate nucleus for suppression of extralemniscal thalamic units.


Effect of potassium ions on glucose and phospholipid metabolism in the rat's cervical sympathetic ganglia with and without axotomy.


Spontaneous bioelectric activity in long term cultures of the embryonic insect central nervous system.


A nucleoside diphosphate phosphohydrolase present in a coated-vesicle fraction from synaptosomes of guinea-pig whole brain.


Syncardial massage in diabetic and other neuropathies of lower extremities.


The kinetics of the anaerobic reduction of fungal laccase B.


Specificity of aeromonas aminopeptidase toward oligopeptides and polypeptides.


Assay of yeast enzymes in situ. A potential tool in regulation studies.


The inhibitory effect of AMP on the activation reactions of the amino acids involved in gramicidin S biosynthesis.


Enzymes of Bacillus licheniformis in the biosynthesis of Bacitracin A.


Selective cleavage of the single tryptophanyl peptide bond in horse heart cytochrome c.


Reaction of sulfenyl halides with cytochrome c. A novel method for heme cleavage.


An intermediate in the reaction of reduced laccase with oxygen.


Cytoplasmic DNA's consisting of unique nuclear sequences in hamster cells. A possible role in cell differentiation and oncogenesis.


Glycolipid synthesis in baby-hamster-kidney fibroblasts transformed by a thermosensitive mutant of polyoma virus.


Effects of hormones on cyclic AMP release in perfused rat livers.


In vitro studies on the acylation of 1-O-alkenyl glycero-3-phosphorylethanolamine by rat brain preparations.


Evidence for an insulin receptor in muscle.


The proton relaxation enhancement properties of concanavalin A.


Characterization of the specific androgen receptors in the human prostate gland.


Effect of perphenazine during late pregnancy on prolactin production and lactogenesis in the rat.


Effect of stress and environmental temperature on adrenal function in Rana esculenta.


Effect of human chorionic gonadotrophin on testicular 5 -adnrostane-3 -hydroxysteroid dehydrogenase, 3 -hydroxydehydroepiandrosterone dehydrogenase and alcohol dehydrogenase of immature rats.


Further studies on the trophic effect of cyclic GMP on rat adrenal cortex.


Intrarenal teratoma occurring in infancy: report of a case with discussion of extragonodal germ cell tumors in infancy.


The impacts of NIMH grants to improve state hospitals.


Isolation and identification of trachoma and inclusion conjunctivitis agents in Melbourne, 1966 to 1972.


Gas gangrene--a ten-year survey from the Royal Adelaide Hospital.


The influence of state regulatory processes on mental health programs.


Implications of psychological research for smoking control clinics.


Treatment of testicular tumors.

Treatment of testicular tumors.


Mesodermal mixed tumor of the corpus uteri.


ECS produced disruption or facilitation of discriminated active avoidance performance in rats.


Estrogen-binding to pancreas.

Estrogen-binding to pancreas.


Trypsin aerosol treatment of pulmonary alveolar proteinosis. Case report.


Studies on uterine flushings in the baboon. II. The effect of an intrauterine contraceptive device on certain biochemical parameters. This paper reports on the concentration of total protein, reducing sugar, and various enzymes in uterine flushings obtained from 17 mature, regularly cyclic baboons and stored at -10 degrees C until required.  It was found that the levels of total protein and reducing sugar was high at both the early proliferative and late secretory stages of the menstru al cycle and fell to their lowest levels in the early secretory phase.  When an IUD was in situ, higher levels of these 2 constituents occurred.  Of the enzymes measured in the early secretory phase, only hemoglobinase was significantly elevated in the presence of an IUD.  Trypsin, chymotrypsin, lysozyme, amylase, and phosphatase were usually detectable in the flushes, but none was significantly altered by the device.  The possible biologic implications of these findings are discussed.


Modified hormonal control of enzymatic activity in tumors.


Isoproterenol and glucagon effects in perfused hearts from spontaneously hypertensive and normotensive rats.


Effect of sodium n-dipropylacetate on audiogenic seizures and brain  -aminobutyric acid level.


The uptake of (14 C) glucose into rabbit ear cartilage proteoglycans isolated by differential extraction and by collagenase digestion.


The Hermansky-Pudlak syndrome: inclusions in circulating leucocytes.


The biochemistry of ferritin.

The biochemistry of ferritin.


A genetically determined disorder with features both of thalassaemia and congenital dyserythropoietic anaemia.


Hypophysis and function of pancreatic islets. II. The effect of substitution with growth hormone and corticotrophin on insulin secretion and biosynthesis of proinsulin and insulin in isolated pancreatic islets of hypophysectomized rats.


Toxicology's need for new analytical methods.


Distribution and localization of sites of gamma aminobutyric acid metabolism in the adult rat brain.


A radiographic study of the growth of the rat pelvis.


Synthesis and biological effects of 13-dehydro derivatives of natural prostaglandin F 2   and E 2  and their l5-epi enantiomers.


Antiparasitic nitroimidazoles. 3. Synthesis of 2-(4-carboxystyryl)-5-nitro-1-vinylimidazole and related compounds.


Synthesis and biological evaluation of LH and FSH releasing hormone and its analogs.


The effects of corticosterone, 18-OH-DOC, DOC and 11 -hydroxyprogesterone on the adrenal pituitary axis of the stressed rat.


Influence of protein under- and overnutrition on spontaneous tumor prevalence in the rat.


In vivo and in vitro testosterone metabolism by the androgen insensitive rat.


Gas chromatographic determination and mass spectrometric identification of oestrogens in normal human placental tissue at term.


Differences between rats and chickens in response to synthetic glucocorticosteroid.


Tumours of the glomus jugulare.

Tumours of the glomus jugulare.


Metabolism of ( 3 H)-testosterone by the adrenal of the dog: the action of ACTH.


Right-sided abscess in the region of the diaphragm associated with bronchiectasis.


Hydrocortisone and vascular reactivity in dogs with hemorrhagic shock.


Humoral factors influencing cell-mediated immune responses to tumour-associated antigens.


Descending pathways in the spinal cord affecting sympathetic activity in spinalized cats.


The problem of "sympathetic tone" with particular respect to sleep.


Optimization aspects in biological control theory.


A rapid electrophoretic technique for identification of subunit species of apoproteins in serum lipoproteins.


Atomic absorption determination of zinc, copper, cadmium, and lead in tissues solubilized by aqueous tetramethylammonium hydroxide.


Alternative methods of preparation and analysis of quasicrystalline protein from Neurospora crassa.


Mathematical model of the neuron used for analysis of periodically modulated patterns of discharges.


Preparation of protohemin monomethyl ester.


An improved cycling assay for nicotinamide adenine dinucleotide.


A new electrophoretic-autoradiographic method for the visual detection of phosphotransferases.


The changes of noradrenaline in sympathetic ganglia and in the brain under reflex and humoral influences.


A note on the functional significance of phosphatases in the club cells of a fresh-water teleost, Saccobranchus fossilis.


Muscarinic inhibition of the release of the adrenergic transmitter from peripheral sympathetic fibres.


Quantitative aspects of the release of noradrenaline from peripheral adrenergic neurones.


Frequency response analysis of inotropic nerve action upon the isolated sympathetically innervated guinea-pig atrium.


Inhibition of ACTH-induced differentiation of cortical cells and their mitochondria by corticosterone in tissue culture of fetal rat adrenals.


Morpho-physiological consideration on synaptic transmission in the amphibian sympathetic ganglion.


Ablation therapy for hormone-dependent tumors.


Importance of axonal transport of new amine granules for  3 H-noradrenaline retention capacity of adrenergic nerve terminals.


A possible role of the catecholamine-containing neurons of the brain-stem of the cat in the sleep-waking cycle.


The utilization of zwitterionic buffer system in the plaque assay of a feline calicivirus.


The reaction of products of sequential periodate oxidation of human erythrocyte glycoproteins with hemagglutinin from Helix pomatia.


Femoral neuropathy due to retroperitoneal bleeding. An unusual complication of heparin therapy.


Chemical mediation of hormone action.

Chemical mediation of hormone action.


The recognition of viruses of the avian leucosis-sarcoma group and other viruses that interfere with Marek's disease research.


A review of arthropod-borne virus infections affecting man and animals in Australia.


Analytical methods for mycotoxins.

Analytical methods for mycotoxins.


Lesions of renal glomeruli in mice with virus-induced diabetes mellitus-like disease.


A radioimmunoassay for alpha 1 -foetoprotein.


Biochemical and morphological investigations of 30 human insulinomas. Correlation between the tumour content of insulin and proinsulin-like components and the histological and ultrastructural appearance.


Articular chondrocalcinosis. Microanalysis of pyrophosphate (PPi) in synovial fluid and plasma.


Antigenic relationships among four herpesviruses. Common viral antigens were detected, by fluorescent-antibody studies, in cells infected with herpes simplex virus 1, squirrel monkey herpesvirus 1, bovine rhinotracheitis, and equine abortion viruses. The two primate viruses showed slight cross-neutralization.


Host defense mechanisms against Herpes simplex virus. I. Control of infection in vitro by senstized spleen cells and antibody. Sensitized mouse spleen cells decrease the spread of herpes simplex virus infection in cell culture lines derived from human and murine tissues. These washed, sensitized cells act alone and additively in combination with antibody to diminish the ability of single virus-infected cells to spread infection to contiguous cells. This control of infection is not species specific, unlike interferon, and appears to be distinct from the effect of antibody. Lymphotoxin was not detected in this lymphocyte-mediated response. This control of herpes simplex virus infection in vitro by sensitized lymphoid cells is immunologically specific; spleen cells from donor animals immunized with a heterotypic virus do not cause herpesvirus plaque size reduction. The ratio of spleen cells from immunized animals to target monolayer cells needed to produce this effect is > 4:1. Plaque size reduction of herpes simplex virus by spleen cells requires intact, immune, non-glass-adhering lymphoid cells.


Leukocytosis-promoting factor of Bordetella pertussis. II. Biological properties. The leukocytosis-promoting factor (LPF), purified from materials extracted from agar in which Bordetella pertussis was grown, caused leukocytosis and an increased sensitivity to histamine when as little as 0.04 mug of protein was given intravenously to mice. LPF was adsorbed onto erythrocytes, causing hemagglutination. As little as 0.03 mug of LPF protein agglutinated chicken erythrocytes in 0.05 ml of a 0.5% suspension. The physicochemical, biological, and immunological properties indicated that leukocytosis, increased sensitivity to histamine in mice, and agglutination of erythrocytes are functions of a single substance.


Isolation and characterization of an Avian adenovirus-associated virus. An 18- to 20-nm virus particle was isolated from the Olson strain of quail bronchitis, an avian adenovirus. On density gradient separation the small virions were primarily found at densities of 1.39 and 1.42 g/cm(3). The majority of the infectious particles were at the heavier density. The virus had a hexagonal outline and contained single-stranded deoxyribonucleic acid. It was resistant to heating at 56 C for more than an hour and was not inactivated by treatment with chloroform or low pH. Purified virus did not agglutinate erythrocytes of various avian and mammalian species. Replication of the small particles occurred either in chicken embryos or in cultures of embryo kidney cells coinfected with an adenovirus helper. Antigenically the virus was distinct from the adeno-associated viruses types 1, 2, 3, and 4. The virus is the avian equivalent of the adeno-associated viruses of primates and lower animals.


Experimental viral arthritis induced with herpes simplex.


Cytomegalovirus infections during acute childhood leukemia.


Antimetabolites of coenzyme Q. XV. Inhibition of mitochondrial reductase systems by naphthoquinone and quinolinequinone analogs.


Isolation and comparison of bovine parvoviruses.


Responses of gibbons to inoculation of Epstein-Barr virus.


Dynamics of the splenic erythrocyte reservoir in the dog.


Activation and inhibition of blood platelet adenylate cyclase by adenosine or by 2-chloroadenosine.


Diet in the prevention of heart disease.


The in vitro sensitivity of fish brain ATPases to organochlorine acaricides.


Rhinoviruses.

Rhinoviruses.


Specificity of the control of protein synthesis by haemin.


Comparison of free and 80S RNA-associated RNAs of mouse L cell virions.


Influence of dibutyryl cyclicAMP and theophylline on cell surface antigens on oncornavirus transformed cells.


Induction of steroidogenesis in tissue culture by cholera enterotoxin.


Adrenergic receptors of the heart.

Adrenergic receptors of the heart.


Molecular mechanisms of hormone receptors.


Isolation and characterization of hormone receptors.


Reduced high affinity 5 -dihydrotestosterone receptor capacity in the ventral prostate of the aging rat.


Polydactyly in American negroes and whites.


Crisis, conversion, and cult formation: an examination of a common psychosocial sequence.


Follow-up of adolescents treated in a psychiatric hospital. I. A review of studies.


Criterion approach to patient care evaluation.


Urinary cyclic AMP in periodic catatonia.


Collaborative provision of aftercare services.


Evaluation of agar-gel double diffusion for the diagnosis of adenovirus infection. Agar-gel double diffusion was compared to the Laboratory Branch complement fixation test. The efficacy of the agar-gel double diffusion test for the semiquantitation of group-specific adenovirus antibody and its usefulness as a serodiagnostic aid for the evaluation of adenovirus infection was determined.


Device for automatic rapid harvest of roller culture supernatant fluid. A machine has been designed to remove and replace supernatant fluids automatically from roller culture bottles at frequent intervals.


Role of the lung in angiotensin metabolism.


Bilateral effects of the afferent impulses from the masseteric muscle on the trigeminal motoneuron of the cat.


Intrawell absorption of antisera in a template thin-film gel diffusion method. This report describes a method for in situ absorption of antisera within the template wells used in thin-film microgel diffusion. The intrawell absorption of antisera offers several advantages over previously reported techniques for detecting the presence of given antigens in biological preparations. The procedure is time saving, antigens are conserved, optimum antigen-antibody ratios are easily determined, and tests of several different absorbing antigens can be made on a single plate.