An aphasic syndrome in children.

An aphasic syndrome in children. The paper presents case reports of nine children, all of whom had severe disturbances of language function of abrupt or gradual onset. Seizures occurred in seven of the children and all nine had EEG abnormalities.There was no apparent correlation between the seizures, the EEG changes and the aphasia. One patient had a brain biopsy performed at another hospital and the tissue was said to be normal. The cause of the abnormal language function is unknown. Five of the children failed to acquire normal speech subsequently. Three of the four children who made a complete recovery were treated with steroids.


The structure of a dextran produced by Leuconostoc mesenteroides NRRL B-1397: the linkages and length of the branches.


Proceedings: Unsaturated fat and plasma triglyceride metabolism in man.


Proceedings: The effects of exogenous parathyroid hormone on circulating adenosine 3':5' monophosphate in man.


The pressor response to intravenously infused angiotensin II: correlation with plasma renin activity.


Action of angiotensin antagonists and antiserum upon the pressor response to renin: further evidence for the local generation of angiotensin II.


Proceedings: Inhibition of adenyl cyclase by a bacterial exotoxin.


The charade of poverty funding for community mental health centers.


Competitive protein-binding assay for 25-hydroxycholecalciferol.


Comparison of pelvic osteotomies for the surgical correction of the congenital hip.


Short-term heroin detoxification on an outpatient basis.


Fibroblast collagenase partial purification and characterization.


A serological study on the occurrence of some respiratory infections in Fiji.


Corneal ulceration and the serum antiproteases. I. Alpha 1-antitrypsin.


Proceedings: Molecular aspects in comparative endocrinology.


The "new" vegetarians. Group affiliation and dietary strictures related to attitudes and life style.


Corneal toxicity of collagenase inhibitors.


Vitamin E: relationship to thyroxine in man.


An experiment in liaison psychiatry to a community health center.


Sudden infant death syndrome: an epidemiologic study.


A biologically active insulin analog with an enlarged intrachain cyclic system.


The role of the kidney and vitamin D metabolism in health and disease.


Susceptibility of the VERO line of African green monkey kidney cells to human enteroviruses. The relative susceptibility of VERO cells and primary rhesus monkey kidney cells to 47 prototype strains of human enteroviruses is described. Of these strains, types 4, 14, 16, 17, 18, 21, 31 and 34 and Coxsackie virus A 9 failed to cause CPE in the VERO cells whilst only one, echovirus type 34, failed to cause CPE in the monkey kidney cells.A comparison is given of the efficiency of the two cell cultures for enterovirus isolation from clinical material. Results show that VERO cells are as useful as primary monkey kidney for the isolation of Coxsackie B viruses but less satisfactory for isolating echoviruses. They are satisfactory for the isolation of single types of poliovirus and appear to be more satisfactory than primary monkey kidney cells for the isolation of mixtures of polioviruses. The identification of enteroviruses by neutralization tests in VERO cells is successful.


The airborne excretion by pigs of swine vesicular disease virus. The air of loose-boxes holding pigs affected with swine vesicular disease was sampled for virus. In the multistage impinger virus to a titre of 10(2.6) TCID 50 was associated with particles greater than 6 mum., 10(1.6) with particles 3-6 mum. and 10(1.4) or less with particles less than 3 mum. In the noses of workers in contact with the pigs for periods not less than 5 min., virus to a titre of 10(2.4) TCID 50 was found. Virus was recovered from the air for 2-3 days during the disease and maximum titre in pigs infected by injection or by contact occurred on the second to third day after generalization of the lesions. The amounts of virus were about 160-fold less than those recovered from pigs affected with foot-and-mouth disease, and the quantity and time of excretion suggest that the source of swine vesicular disease virus in the aerosol may be from the lesions and skin rather than from the respiratory tract.


Arbovirus serology in North Dakota mule and white-tailed deer.


A study of reproduction, disease and physiological blood and serum values in Idaho elk.


Antibody responses in patients with farmer's lung disease to antigens from Micropolyspora faeni. Three purified cell-wall glycoprotein antigens, a, b and c, sensitive to sodium periodate and resistant to pronase, were extracted from mycelium of Micropolyspora faeni with aqueous phenol or trichloracetic acid. Pronase-sensitive, sodium periodate-resistant protein antigens were extracted from mycelium with aqueous phenol. Immunoelectrophoresis was a critical method of assessing purity of preparation.Antibodies to glycoprotein antigens only occurred in clinically defined cases of farmer's lung disease, whereas antibodies to protein antigens also occurred in symptomless farmers. Precipitins to a occurred in all cases of farmer's lung disease, and this antigen was isolated and purified.Fractionation of pooled sera from cases of farmer's lung disease showed that precipitins were IgG globulins, whereas latex-agglutinating antibodies were IgG, IgA and especially IgM globulins. It is suggested that farmer's lung may involve a cytotoxic type II reaction, in which glycoproteins adsorbed to tissue cells react with IgG, IgA and IgM immunoglobulins in the presence of complement, causing cellular damage.


Vaccination of American bison against Pasteurella multocida serotype 2 infection (Hemorrhagic septicemia).


Neutralization of channel catfish virus by serum of channel catfish.


Electron microscopy of the virus of oral papillomatosis in the coyote.


Cholesterol metabolism in the rat sensitive to high cholesterol diet.


A fatal case of type 2 herpes simplex virus infection in a newborn.


Changes in back-transplantability by fluid-suspension culture and by long-term of cultivation of two tissue culture cell strains, JTC-1 and JTC-2, originated from rat ascites hepatoma AH-130.


Humoral cytotoxic factor in isograft immunity against azo-dye induced hepatoma.


Primary hyperparathyroidism: clinical and biochemical features.


Electron microscopy of bluetongue virus RNA.


Foot-and-mouth disease in the african elephant (Loxodonta africana).


Total decompression of the facial nerve in osteopetrosis (marble bone disease-morbus Albers-Schönberg).


Hodgkin's disease: considerable progress and more to be achieved.


Plasma cortisol and the use of hydrocortisone in the treatment of status asthmaticus.


Electron microscopy of chlorphentermine lung.


Granular cell myoblastoma of the oesophagus.


Studies in cholestasis of pregnancy with special reference to lipids and lipoproteins.


Cyclic nucleotide phosphodiesterases.

Cyclic nucleotide phosphodiesterases.


Certain examples of application of ascorbate in biochemical investigations.


Nonhuman primates in viral oncology.

Nonhuman primates in viral oncology.


Intestinal diamine oxidase: isolation, substrate specificity and pathophysiological significance.


Comparison of the 14-C-putrescine assay with the NADH test for the determination of diamine oxidase: description of a standard procedure with a high precision and an improved accuracy.


The intimal proliferation in aortic-coronary saphenous vein grafts. Light and electron microscopic studies.


Copper heterogeneity in pig kidney histaminase. An EPR study on the effect of substrate, guanidine and mersalyl.


Cyclic adenosine monophosphate and clinical medicine. I.


Effects of angiotensin I and angiotensin II on renal blood flow in sheep.


Determinants of systemic hydraulic power in unanesthetized dogs.


Intrauterine virus infections and congenital heart disease.


Plasma 17-hydroxycorticosteroids and growth hormone during defense reactions.


Effect of melanotropic peptides on protein synthesis in mouse brain.


An antimelanotropic protein in human cerebrospinal fluid.


Orbital space-occupying lesions. Four decades of Eye Hospital reports.


Isolation and lipid composition of spinach chloroplast envelope membranes.


Purification and characterization of the Novikoff hepatoma glycogen phosphorylase and its relations to a fetal form.


Simultaneous measurement of oxidative phosphorylation and adenylate kinase in plant mitochondria.


Structural requirements for the formation of ordered lipid multibilayers--a spin probe study.


Homotropic regulation of dihydrolipoyl dehydrogenase from bovine kidney.


On the mechanism of a calcium-associated defect of oxidative phosphorylation in progessive muscular dystrophy.


Enzymes of serine biosynthesis in Rhodopseudomonas capsulata.


Respiratory changes induced by guanidines and cations in submitochondrial particles.


An assessment of some molecular parameters of mevalonate kinase from plant and animal sources.


The oxidation-reduction potentials of electron carriers in chloroplast photosystem I fragments.


Cholesterol uptake from doubly-labeled alpha-lipoproteins by cells in tissue culture.


Oat cell carcinoma of the larynx.

Oat cell carcinoma of the larynx.


Enzymatic dehydrogenation of steroids by beta-hydroxysteroid dehydrogenase in a two-phase system.


The mechanism of the diaphorase reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase.


The molecular reorganization of lipid bilayers by osmium tetroxide. A spin-label study of orientation and restricted y-axis anisotropic motion in model membrane systems.


Aspects of Epstein-Barr virus infection in childhood.


Enzyme changes in diabetes mellitus.

Enzyme changes in diabetes mellitus.


Radiosensitivity and the ascorbic acid electron spin resonance doublet.


Depolarization-evoked accumulation of cyclic AMP in brain slices: inhibition by exogenous adenosine deaminase.


Very low density lipoprotein "remnant" particles: uptake by aortic smooth muscle cells in culture.


Effects of insulin on gluconeogenesis and cyclic AMP levels in perfused livers from diabetic rats.


Effects of adrenalectomy and glucocorticoid replacement on gluconeogenesis in perfused livers from diabetic rats.


Vitamin D metabolites. Specific binding by rat intestinal cytosol.


The role of calcium in fly salivary gland secretion analyzed with the ionophore A-23187.


The development and present status of ultrasonic encephalography.


Renal function during and after anaesthesia and surgery: significance for water and electrolyte management.


Hearing levels in Nigerian ataxic neuropathy.


Neuro-audiological correlates in cerebral hemisphere lesions. Temporal and parietal lobe tumors.


Chlamydial infections of the cervix.

Chlamydial infections of the cervix.


Sexually transmitted diseases. Extract from the Annual Report of the Chief Medical Officer of the Department of Health and Social Security for the year 1972.


Chlamydia A in the female genital tract.


Ultrastructural characteristics of Trichomonas vaginalis. An electron microscopical study.


The role of infection in the morbidity of asthmatic children admitted to hospital.


Proceedings: Virological aspects.

Proceedings: Virological aspects.


Proceedings: Aspects of EB virus infection.


Proceedings: EB virus and other diseases.


Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin.


Cyclic production of vesicular stomatitis virus caused by defective interfering particles.


Three Yugoslav herpes simplex viruses: biologic and antigenic properties and formation of giant cells in vitro by a cervical isolate.


Editorial: Recent contributions to the mechanism of action and clinical relevance of erythropoietin.


The lymphoreticular system and interferon production.


Studies on the function of cell membrane. 8th report. Binding of cytochrome c to liver plasma membrane and microsome in CCl4-poisoned rats.


Neuro-vestibular correlates in cerebral hemispheric lesions.


Studies on the function of cell membrane. 9th report. Protective action of sodium taurine N-carbodithioate (TDT) against CCl4-induced biochemical changes in liver cell membrane of rats.


Participation of muscarinic receptors on splanchnic-adrenal transmission in the rat.


Effect of cytochrome c on carbon tetrachloride damage to the liver.


Effects of low concentrations of lithium chloride on adrenergic transmission in the isolated atria of rabbits.


Potentiation of the contractile response of isolated aortae to transmural stimulation by angiotensin.


Anti-oxytocin actions of papaverine, aspaminol and isoprenaline on the rat uterus and intracellular cyclin adenosine 3,5-monophosphate level.


The role of cyclic nucleotides in visual excitation.


The construction and use of quartz fiber fish pole balances.


Oxidation states of peroxidase.

Oxidation states of peroxidase.


Action of a serum protein in coupling excitation contraction of heart papillary muscle.


Mycobacterium fortuitum abscess related to surgery, malignancy, and a tight girdle.


Bronchiolar-alveolar carcinoma of the lung.


The treatment of ileocolitis with ACTH and prednisone: immediate and prolonged effect.


Current therapy of thyroid nodules.

Current therapy of thyroid nodules.


Medullary carcinoma of the thyroid gland: use of biochemical parameters in detection and surgical management of the tumor.


Surgical management of the Zollinger-Ellison syndrome.


Endocrine surgery in children.

Endocrine surgery in children.


Surgical considerations in gynecologic endocrine disorders.


Radical surgery of tumors of the thalamus, hypothalamus, and third ventricle area.


The ultrastructure of the accessory sex organs of the male rat. 3. The post-castration involuation of the coagulating gland and the seminal vesicle.


The ultrastructure of the accessory sex organs of the male rat. II. The post-castration involution of the ventral, lateral and the dorsal prostate.


Ultrastructure and functional aspects of the nucleus infundibularis ventralis in the green frog, Rana esculenta.


Effect of denaturation on muscle proteins as studied by Epr.


Electron microscopical studies on the structure, phagocytic properties, and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig.


Histoenzymic studies of the central nervous system in tri-ortho-cresyl phosphate poisoning.


Hydrosmotic effect of angiotensin II in the toad bladder: role of cyclic AMP.


Protein synthesis by isolated spermatozoa from cauda and caput epididymis of rat.


Influence of adrenocorticotropic hormone on red cell mass and body composition of the hypophysectomized rat.


Isolation of proteins by gel filtration in 6M guanidinium chloride: application to RNA tumor viruses.


The electrophoretic separation of nucleoside-5'-phosphates from nucleoside trialcohol monophosphates, and its application to the identification of 3'-end groups.


Analysis of steroids in biological fluids by computer-aided gas-liquid chromatography--mass spectrometry.


Fifty years with viruses (Christopher H. Andrewes).


New isothiazole derivatives. II. Cytotoxicity and antiviral activity in tissue cultures.


DNA in heterochromatin cytophotometric pattern recognition image analysis among cell nuclei in duct epithelium and in carcinoma of the human breast.


Comparison of hemodynamic responses to exercise in hypertensive patients and in healthy subjects given angiotensin.


Exogenous hormones in pregnancy.

Exogenous hormones in pregnancy. Various changes that have been observed after intrauterine exposure to exogenous hormones are presented.  Often, alterations observed after exogenous maternal hormone therapy consist of varying degrees of masculinization of the female genitalia.  The following specific drug interactions are described in some detail: 1) effects of progestational agents and androgens on female genitalia; 2) relationship of corticosteroids to the development of cleft palate; and 3) effects of synthetic estrogens (e.g., diethylstilbestrol) on the female reproductive tract.  The discussion closes with a review of the association of therapy with such drugs to the delayed appearance of clear-cell adenocarcinoma of the genital tract in young women.


Gynecologic endocrine disorders in childhood and adolescence.


Multihormonal regulation of hepatic histidase during postnatal development.


Cellular differentiation and isozymes of branched-chain amino acid transaminase.


Control of rat liver phosphorylase, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase activities by insulin and glucagon during the perinatal period.


Development of disaccharidase and phosphatase activities in the intestine and liver.


The regulation of liver development by birth.


Nucleotide supply of the developing animal: role of the so-called 'salvage pathways'.


Development of intestinal function in mammals.


The developmental formation of enzymes involved in glycogen metabolism.


The effects of bases and nucleosides on the intracellular contents of nucleotides and 5-phosphoribosyl 1-pyrophosphate in Escherichia coli.


The regulation of phosphoenolpyruvate carboxykinase in fetal rat liver.


A sequence of 54 nucleotides from the A-protein cistron of coliphage-R17 RNA.


Studies with analogues of nicotinamide adenine dinucleotide.


Early chromosome changes in diploid Chinese hamster cells after infection with Simian virus 40.


Hydrolysis of the cyclic 3':5'-monophosphates of adenosine and guanosine by rat pancreas.


Kinetics of the anti-tumor delayed hypersensitivity response in mice with progressively growing tumors: stimulation followed by specific suppression.


Properties of flat variants of murine sarcoma virus transformed non-producer cells isolated after high-temperature passage.


Syndactyly. A review of the literature.

Syndactyly. A review of the literature.


Differences in the surface mobility between normal and SV40-, polyoma- and adenovirus-transformed hamster cells.


Kinetics of uptake of 2-deoxy-D-glucose and 2-aminoisobutyric acid in chemically transformed cells.


Proteolipids. VI. The dual role of phospholipid in cytochrome oxidase reaction.


Spin-labelling studies of fragmented sarcoplasmic reticulum.


Functional anion binding sites in dogfish M4 lactate dehydrogenase.


Vinblastine in the treatment of carcinoma of liver.


Fine structure of the axon collaterals of Huber in frog spinal ganglia.


Hypermyoinositolemic polyneuropathy in rats. A possible mechanism for uremic polyneuropathy.


Multiple system atrophies. A neuropathological and neurochemical study.


Somatosensory evoked responses to mechanical stimulation in normal subjects and in patients with neurological disorders.


The role of adenosine 3',5'-cyclic monophosphate in the regulation of mammalian cell division.


Paralysis periodica paramyotonica. A clinical and neurophysiological study.


Concentrically laminated membranous inclusions in myofibres of Dyggve-Melchior-Clausen syndrome.


Effects of the microbial environment on drug resistance.


Polyneuropathy in Waldenström's macroglobulinaemia.


New roles for ascorbic acid.

New roles for ascorbic acid.


D-glyceraldehyde-3-phosphate dehydrogenase: three-dimensional structure and evolutionary significance. A 3.0-A resolution electron density map of lobster glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) was computed. The essentially single isomorphous replacement map was very substantially improved by averaging subunits. NAD binds in an open conformation at sites close to subunit interfaces. The coenzyme binding portion of the enzyme has almost the same fold as the corresponding portion of lactate dehydrogenase (EC 1.1.1.27). The presence of this structure in the five enzymes, analyzed so far, that use nucleotide coenzymes might indicate a fundamental primordial structural element.


Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian) cells which normally contain no nucleotide sequences specific for Rous sarcoma virus, transformation by the virus results in the appearance of DNA specific for Rous sarcoma virus covalently integrated into strands of host-cell DNA containing reiterated sequences. Early after infection of mouse or duck cells by Rous sarcoma virus, unintegrated DNA specific for the virus can be demonstrated.


Programming of cells for death under experimental conditions: relevance to the tumor problem. An attempt was made in this study to determine more precisely the nature of the factors that are involved in the programming of cells for a form of terminal cellular differentiation that results in death. These studies demonstrated that both the cytokinesins, which are potent inhibitors of plant and animal adenosine 3':5'-cyclic monophosphate phosphodiesterases, and 8-bromoadenosine 3':5'-cyclic monophosphate, which is a stable, biologically active form of adenosine 3':5'-cyclic monophosphate, are highly effective in encouraging differentiation of parenchyma cells into tracheary elements with accompanying death. Since adenosine 3':5'-cyclic monophosphate and theophylline when used together were also effective, the results reported here suggest that adenosine 3':5'-cyclic monophosphate is somehow importantly involved in the conversion of parenchyma cells into tracheary elements in this system. The possible significance to the tumor problem generally of the programming of cells for terminal differentiation, with or without resulting death, is discussed.


A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase. A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl-tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein-synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.


Charge transfer between amine and carbonyl. The transfer of a whole electron in the ground state can be demonstrated by the interaction of p-phenylenediamine and glyoxal, which leads to the formation of free radicals.


Adenosine-mediated elevation of cyclic 3':5'-adenosine monophosphate concentrations in cultured mouse neuroblastoma cells. Adenosine causes an increase in the concentration of cyclic AMP in mouse neuroblastoma cells. The amount of increase observed in intracellular cyclic AMP levels due to exogenous adenosine depends greatly on the concentration of a specific cyclic AMP phosphodiesterase inhibitor, 4-(-3-butoxy-4-methoxybenzyl)-2-imidazolidinone. Unstimulated concentrations of cyclic AMP were 29-40 pmol/mg of protein, and concentrations after addition of 0.2 mM adenosine were usually twice as high. The presence of 0.7 mM inhibitor along with 0.2 mM adenosine caused an increase in cyclic AMP levels up to 1000-2000 pmol/mg of protein. In the presence of 0.7 mM inhibitor, 2 muM adenosine gives a half-maximal cyclic AMP elevation. Theophylline blocked the elevation of cyclic AMP concentrations caused by exogenous adenosine. The data show that the cyclic AMP system of mouse neuroblastoma has the necessary receptor components to respond positively to exogenous adenosine. The results presented support a direct effect of adenosine, mediated through its control of intracellular levels, on neuronal elements of the nervous system.


Heme proteins: quantum yield determined by the pulse method. We report results of the application of the "pulse method" to the study of photodissociation of various ligands from several heme proteins. By use of this technique, which allows the determination of the quantum yield of photodissociation accurately and rapidly, several ligands (CO, O(2), isocyanides) have been investigated for sperm-whale myoglobin and trout hemoglobin I. In agreement with previous results, the new data lead to the conclusion that no simple relationship exists between the quantum yield and the affinity constant in the ground state. For trout hemoglobin I, the experiments were extended to measure the quantum yield of the CO photodissociation as a function of the initial degree of saturation, from fully saturated down to the initial values of about 1.5%. The results yield additional information to that obtained by the "steady-state" method, and in particular exclude the idea that the photochemical yield is in any way dependent on the fractional saturation of the molecule with carbon monoxide.


Alteration of cell-surface proteins by viral transformation and by proteolysis. Putative cell-surface proteins of tissue-culture cells were identified by lactoperoxidase-catalyzed iodination, a technique that attaches label only to proteins outside the cell membrane. Evidence is presented that these proteins are cell derived, not contaminating serum proteins. On "normal" cells, which exhibit density dependence of growth, one protein of high molecular weight was particularly readily iodinated. This protein was easily removed by mild proteolytic digestion, and, in virus-transformed cells, was either absent or unavailable for iodination. The possible relevance of these observations to the control of growth in cell culture is discussed.


Biological and biochemical evidence for an interaction between Marek's disease herpesvirus and avian leukosis virus in vivo. The DNA-containing Epstein-Barr herpesvirus has been implicated in the etiology of Burkitt's lymphoma, a malignant tumor of children in Africa. Recently, however, particles possessing four biochemical characteristics of RNA tumor viruses have also been identified in these tumors. The fact that both types of viruses are found suggests that an interaction between them may be playing a role in the etiology of Burkitt's lymphoma. To explore this possibility with a defined animal model, experiments were performed with the avian Marek's disease, a malignant lymphoproliferative disease associated with a herpesvirus. Controlled contact studies demonstrated that tumorigenesis in a line of isolator-derived, barrier-sustained, specific pathogen-free chickens requires exposure to both the Marek's disease herpesvirus and an avian leukosis virus, Rous-associated virus, type 2. Exposure to either agent alone did not result in tumors. Molecular hybridization experiments to cytoplasmic RNA from similarly contact-exposed conventional Cornell S-line chickens provided further evidence for the occurrence of an interaction between Marek's disease herpesvirus and the avian leukosis virus.


Pressure effect on the membrane action of a nerve-blocking spin label. A reversible nerve-blocking spin label, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) was used to study the nature of anesthetic-binding sites in nerve membranes as a function of pressure. The nerve-blocking effect of TEMPO is enhanced under pressure. At atmospheric pressure, TEMPO blocks nerve conduction by solubilizing in the apolar region of the nerve membrane. However, the nerve-conduction-block by TEMPO at 150 atm of helium was related to the binding of TEMPO to a pressure-induced high-affinity polar site in the nerve membrane. The new TEMPO-binding site could not be detected in lipid model membranes and, thus, the involvement of membrane protein in the new site was inferred. Pressure may induce a nerve membrane conformation change in the presence of TEMPO. The observation that under different pressure, a single anesthetic, i.e., TEMPO, was capable of blocking nerve conduction by binding to two different sites within the nerve membrane, supports the view that there are multiple anesthetic receptor sites, which differ in chemical composition and location within the nerve membrane. These sites, when occupied by different classes of anesthetics, produce the general phenomenon of nerve-conduction block. The enhancement of nerve-conduction block by pressure may be due to the increased concentration of TEMPO in the new site in the nerve membrane under pressure.


Malignant transformation of BHK21 clone 13 cells by BK virus--a human papovavirus. BK virus, a widespread human papovavirus, is shown to cause malignant transformation of BHK(21) clone 13 cells. The transformed cells grow as tumors upon inoculation in adult hamsters. The question of the possible role of this virus in human neoplasia is raised.


Regulation of glucose synthesis in hormone-sensitive isolated rat hepatocytes. A simplified procedure was developed for isolation of intact, hormone-sensitive liver cells in a high and reproducible yield. These cells produce glucose from various precursors at rates comparable to those achieved in isolated perfused liver. Glucagon enhanced glucose synthesis from pyruvate, dihydroxyacetone, fructose, or xylitol more effectively at low than at high substrate concentration. At high pyruvate concentrations (>2 mM), glucagon or adenosine 3':5'-cyclic monophosphate (0.1 mM) exerts a curious inhibition of gluconeogenesis that can be reverted to stimulation on addition of ethanol. It is suggested that glucagon and cyclic AMP inhibit pyruvate dehydrogenase and thus limit the supply of reducing equivalents needed for glucose formation. Supporting evidence for hormonal control of pyruvate dehydrogenase in isolated liver cells is provided by the fact that glucagon decreases and insulin increases decarboxylation of [1-(14)C]pyruvate. Calcium salts (1.3 mM) enhance glucose formation from pyruvate but greatly enhance the inhibition exerted by the divalent cationophore, A23187. Inhibition by glucagon of glucose synthesis from pyruvate is additive with the effects of A23187 + Ca(++). However, with dihydroxyacetone as substrate, glucagon partially reverses the inhibition exerted by A23187 + Ca(++). The results are consistent with glucagon effecting an inhibition of pyruvate dehydrogenase and a stimulation of hexosediphosphatase activities.


Herpes simplex and herpes genitalis viruses in etiology of some human cancers. The results of complement fixation tests on 202 sera from people without cancer and from patients with cancer in 29 different areas of the body indicated that only those with nine varieties of advanced cancer (lip, mouth, oropharynx, nasopharynx, kidney, urinary bladder, prostate, cervix uteri, and vulva-all of 56 tested) gave positive specific reactions with nonvirion antigens induced by the DNA herpes simplex (HSV 1) and herpes genitalis (HSV 2) viruses. None of 57 people without cancer (including 10 with current and 18 with recurrent HSV 1 or HSV 2 infections), none of 81 patients with 20 other varieties of advanced cancer (gum, tongue, tonsil, salivary gland, accessory sinus, epiglottis, lung-bronchus, stomach, colon, breast, corpus uteri, ovary, testis, liver, thyroid, Wilms' embryonal kidney, melanoma, Hodgkin's disease, acute lymphocytic leukemia, and acute myelocytic leukemia), and none of four women with early malignant changes in the cervix uteri gave positive results. The seven patients with advanced cancer of the lip or oropharynx gave positive reactions with HSV 1 but not with HSV 2 nonvirion antigens (compatible with involvement of only HSV 1), all of the 13 women with advanced cancer of the cervix uteri and the one woman with advanced cancer of the vulva gave positive reactions with both HSV 1 and HSV 2 nonvirion antigens (compatible with involvement of only HSV 2), while among the 35 other positive patients only two (one with cancer of the kidney and one with cancer of the bladder) reacted with HSV 1 and not at all with HSV 2 nonvirion antigens. Positive sera failed to react with cells harvested at different times after high-multiplicity infection with the DNA vaccinia virus. Massive absorption of positive sera with trypsinized, uninfected human embryonic kidney cells failed to remove, or lower the titer of, the HSV 1 and HSV 2 nonvirion antibodies. All of these data taken together are interpreted as indicating that HSV 1 and HSV 2 play an etiologic role in certain human cancers, because they provide the kind of evidence by which virus-free experimental cancers can be proved to have been originally induced by such DNA viruses as polyoma, Simian Virus 40, or certain types of adenovirus.


Separate intramolecular pathways for reduction and oxidation of cytochrome c in electron transport chain reactions. The monoiodotyrosine 74, formyltryptophan 59, mononitrotyrosine 67, and carboxymethylmethionine 80 derivatives of horse cytochrome c are defective in their ability to accept electrons from the succinate-cytochrome c reductase system, while their reactions with purified cytochrome c oxidase are essentially those of the native protein. The 4-nitrobenzo-2-oxa-1,3-diazole derivative of lysine 13 of horse cytochrome c and the bis-phenylglyoxal derivative of arginine 13 of Candida krusei cytochrome c have the opposite properties, in that they are readily reduced by the succinate-cytochrome c reductase (EC 1.3.99.1) system but are defective in their capability of transferring electrons to cytochrome c oxidase (EC 1.9.3.1). We conclude that electrons from mitochondrial cytochrome c reductase are transmitted to ferricytochrome c by a different pathway than electrons from ferrocytochrome c to cytochrome c oxidase. The present results are compatible with the concept that the mechanism of reduction involves an aromatic ring channel comprising residues 74, 59, 67, and 80, leading from the "left back" part of the protein to the heme iron. On the other hand, since residue 13 is immediately above the edge of the heme that is at the "front surface" of the molecule, we suggest that the electron leaves ferrocytochrome c to cytochrome c oxidase by way of the edge of pyrrole ring II or the adjacent surface-located sulfur of cysteinyl residue 17, which is thioether bonded to the heme. On this basis, the sites of electron entry and exit in cytochrome c would appear to be some 110 degrees of arc away from each other along the surface of the protein, explaining several previously observed phenomena.


Plasma membranes from cultured muscle cells: isolation procedure and separation of putative plasma-membrane marker enzymes. Partially purified plasma membranes were obtained from chick-embryo muscle cells grown in tissue culture. The purification procedure involved homogenization in buffered isotonic sucrose followed by differential and sucrose density gradient centrifugations. The activities of five plasma-membrane markers, as well as microsomal and mitochondrial markers, were followed throughout the purification. When cultures were labeled with [(125)I]alpha-bungarotoxin, which binds to the surface of cultured muscle cells, the distributions of bound alpha-bungarotoxin and Na(+),K(+)-ATPase (EC 3.6.1.3) activity were nearly identical. The activities of these two plasma-membrane markers were maximal in the upper two fractions of the sucrose density gradient and were purified 5- to 7-fold with respect to total particulate protein. These fractions contained 20-30% of the Na(+),K(+)-ATPase activity and bound alpha-bungarotoxin, 4% of the microsomal marker TPNH-dependent cytochrome c reductase, 0.2% of the mitochondrial marker succinate-dependent cytochrome c reductase, 2.7% of the cellular RNA, and 0.02% of the DNA. The activity of the commonly used plasma-membrane marker, 5'-nucleotidase (EC 3.1.3.5), was low in the upper two sucrose gradient fractions and was maximal in a more dense fraction. The distributions of the other two plasma-membrane markers, leucyl beta-naphthylamidase and phosphodiesterase I, were intermediate between Na(+),K(+)-ATPase and 5'-nucleotidase. The distributions of all markers were similar in preparations from cultures containing mononucleated myogenic cells, multinucleated myotubes, fibroblasts, or all three cell types. Modification of the procedure to include homogenization in the absence of sucrose resulted in a 3.4-fold purification of the membranes containing 5'-nucleotidase, which were shifted to a lower density.


DNA of Epstein-Barr virus detected in tissue of Burkitt's lymphoma and nasopharyngeal carcinoma. The association of Epstein-Barr virus (EBV) DNA with African tumors was examined by the method of hybridization with complementary RNA. 22 out of 23 biopsies of Burkitt's lymphoma and 18 out of 23 biopsies of nasopharyngeal carcinoma were found to contain EBV DNA. The number of EBV genome equivalents per cell ranged from four to 113. Five out of 24 other African tumors, two adenocarcinomas, and one each of melanoma, reticulum cell sarcoma, and carcinoma of the antrum, also contained EBV DNA.


A four-year clinical and serologic study of the use of inactivated parainfluenza-3 virus vaccines and Pasteurella sp. bacterins in beef calves.


On the nature of frequency dependence of the evoked potentials in the cat's auditory cortex.


Effect of conformational changes on the enzymatic inactivation of pig and rabbit skeletal muscle phosphorylase-a.


Plasma vitamin E and cholesterol relationship in Western Canadian Indians.


Relationship of ethanol to choline metabolism in the liver: a review.


Letter: Herpesvirus type 2 infection and cervical carcinoma.


Guanine nucleotides and their significance in biochemical processes.


In vitro viral studies of cells grown from human amniotic fluid samples.


Height, weight, and lines of arrested growth in young Guatemalan children.


Amebic liver abscess following metronidazole therapy for amebic colitis.


Synergistic interaction of two agents in mice: Japanese B encephalitis virus and Trichinella spiralis.


Needle-like cytoplasmic inclusions in the liver in prophyria cutanea tarda.


A proposal linking atherogenesis to the interaction of endothelial lipoprotein lipase with triglyceride-rich lipoproteins.


Impaired left ventricular function in alcoholic cirrhosis with ascites. Ineffectiveness of ouabain.


Chromatin-bound kinase activity and phosphorylation of chromatin non-histone proteins during early kidney regeneration after folic acid.


Adhesion of culture cells to their substratum.


An unusual cytoplasmic iron-binding material that disappears from diploid cells placed in culture.


Inhibition by cyclic AMP dibutyryl cyclic AMP of aggregation of embryonic quail liver cells in culture.


Intracellular antigen migration in interspecific myoblast heterokaryons.


Stimulation of 2-deoxyglucose uptake in growth-inhibited BALB-C 3T3 and revertant SV40-transformed 3T3 cells.


Subfractionation and properties of rat kidney cortex microsomal fraction.


Estrogen sensitive cell lines: establishment and characterization of new cell lines from estrogen-induced rat pituitary tumors.


Heterogeneity of the cell population of the cellular slime mold Dictyostelium discoideum before aggregation, and its relation to the subsequent locations of the cells.


The relationship between concentration of prostaglandin E and rates of cell replication.


Myocardial lesions in the offspring of female rats fed a copper deficient diet.


Stimulating effect of crystalline cholesterol on spermiogenesis and oxidative activity in the senile rat testicle.


Effect of suramin in doses blocking trypan blue endocytosis on some enzymes in mouse liver.


Technique of electron spin resonance investigations in the x-ray field.


Local antibody response to experimental poliovirus infection in the central nervous system of rhesus monkeys. By employing the techniques of immunofluorescence and radioimmunodiffusion using (32)P-labeled poliovirus as the antigen, the immunoglobulin response to poliovirus in serum, nasopharynx, spinal fluid, and in different segments of the central nervous system (CNS) was studied after intramuscular, oral, intranasal, and intrathalamic administration of inactivated (Salk), live attenuated (Sabin), or live virulent (Mahoney) type I poliovirus. Spinal fluid gammaG antibody was detected after immunization with Sabin or Mahoney virus and intramuscular administration of Salk vaccine. The response in the CNS was characterized by the appearance of gammaG antibody after oral or intrathalamic administration of Mahoney virus and rarely after intrathalamic inoculation of Sabin vaccine. The antibody activity in CNS was limited to the areas of poliovirus replication. Intrathalamic immunization with Mahoney virus resulted in local gammaG antibody production in the CNS in the absence of any detectable response in serum. Discrete foci of gammaG-containing cells were observed in those areas of CNS which contained poliovirus antibody. No immunoglobulin-containing cells or poliovirus antibody was seen in the CNS of monkeys immunized with intramuscularly or orally administered Sabin or Salk vaccine and in sham-immunized control monkeys. It is suggested that the CNS, when stimulated locally with a potent replicating viral antigen, may manifest a specific local antibody response, which is independent of the response in serum.


California arbovirus (La Crosse) infections. II. Precipitin antibody tests for the diagnosis of California encephalitis. Precipitin antibodies to California group arboviruses were studied by double agar gel immunodiffusion (ID) and counterimmunoelectrophoresis (CEP). Forty children with California encephalitis (CE), 12 patients with other forms of meningoencephalitis, and 120 residents of endemic CE areas were tested. Precipitin antibodies were detected only in the 40 patients with CE, whereas hemagglutination-inhibiting, neutralizing, and complement-fixing antibodies were also found in persons without a history of CE. Precipitin antibodies did not persist for more than a year, in contrast to the other three antibodies which may be detected for 2 or more years. Of the children with CE, 36% had precipitin antibodies in their acute sera by CEP and 44% by ID. Precipitin responses were subtype-specific, and the patients reacted most strongly with La Crosse virus. The results indicated that precipitin techniques would be useful for the early detection of CE in some patients and that the presence of precipitin antibodies in a patient with acute central nervous system infection is strongly suggestive of CE. In addition, precipitin tests can be used to distinguish the California subtype responsible for CE in a given area.


Continuous cyclic hormonal therapy.

Continuous cyclic hormonal therapy.


Role of interferon in murine lactic dehydrogenase virus infection, in vivo and in vitro. The induction of interferon (IF) by lactic dehydrogenase virus (LDV) and the action of exogenous IF on LDV multiplication have been studied in vivo and in vitro. In vivo the induction of serum IF by LDV was shown to correspond with the virus challenge dose. In vitro LDV-infected macrophages did not produce detectable IF. As to the action of IF in vivo, the prophylactic or therapeutic administration of IF to mice reduced the LDV serum titers only temporarily. In vitro, (i) the sensitivity of LDV to IF in macrophage culture was 30 times less than that of vesicular stomatitis virus, and 5 times less than that of Sendai virus, and (ii) prolonged exposure of LDV-infected macrophages to high IF concentrations did not cure the chronic infection, but only decreased the virus titer. The probable role of IF in the establishment of the early phase of a persistent but reduced viremia in mice is discussed.


The distribution of transfer ribonucleic acid in rabbit reticulocytes. Levels of aminoacylation and ribosomal attachment during hemoglobin synthesis.


Characterization of a proposed oxytocin receptor in the uterus of the rat and sow.


The mechanism of end product inhibition of serine biosynthesis. IV. Subunit structure of phosphoglycerate dehydrogenase and steady state kinetic studies of phosphoglycerate oxidation.


A new apoprotein of human plasma very low density lipoproteins.


Rat brain tubulin and protein kinase activity.


The involvement of serine and carboxyl groups in the activity of bovine pancreatic deoxyribonuclease A.


Chicken liver phosphofructokinase. 3. Kinetics and allosteric properties.


Regulation of mitochondrial alpha-ketoglutarate metabolism by product inhibition at alpha-ketoglutarate dehydrogenase.


A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. II. Further studies on the substrate specificity.


Membrane glycopeptides from subcellular fractions of control and virus-transformed cells.


The cytoplasmic estradiol receptors of bovine uterus. Their occurrence, interconversion, and binding properties.


Synthesis of virus-capsid antigen (VCA) enhanced by ultraviolet irradiation of a lymphoblastoid cell line carrying Epstein-Barr virus.


Stimulation of hydrolysis of phosphatidic acid by cholinergic agents in guinea pig synaptosomes.


Elevated inorganic pyrophosphate concentrations in synovial fluids in osteoarthritis and pseudogout.


The effect of cyanate upon 2,3-diphosphoglyceric acid metabolism.


Oxidative energy metabolism in the skeletal muscle: biochemical and ultrastructural evidence for adaptive changes.


Differential methylation of mitochondrial and nuclear DNA in cultured mouse, hamster and virus-transformed hamster cells. In vivo and in vitro methylation.


Half-of-the sites reactivity in the catalytic mechanism of yeast glyceraldehyde 3-phosphate dehydrogenase.


Molecular basis of negative co-operativity in rabbit muscle glyceraldehyde-3-phosphate dehydrogenase.


Secondary structure in heterogeneous nuclear RNA: involvement of regions from repeated DNA sites.


Letter: In vitro polyoma DNA synthesis: involvement of RNA in discontinuous chain growth.


Accumulation of single-stranded regions in DNA and the block to replication in a human cell line alkylated with methyl methane sulfonate.


Dependence of the sedimentation coefficient of denatured closed circular DNA in alkali on the degree of strand interwinding. The absolute sense of supercoils.


Cystic meningiomas simulating astrocytomas. Report of three cases.


In vitro polyoma DNA synthesis: discontinuous chain growth.


Evidence for a vasopressor substance (renin) in human fetal kidneys.


Prevention of malignant hypertension by the synthetic peptide SQ 20,881.


Persistent herpes simplex virus infection in rabbit trigeminal ganglia.


Ultrastructure of the glomerular basement membrane in long term renal allografts with transplant glomerular disease.


Letter: Hyperphosphatemia and hypocalcemia in neoplastic disorders.


Letter: Crystalline perinuclear inclusions in myoblasts from human dystrophic muscle.


Analytical zonal centrifugation of synaptosomes and mitochondria from rat brain homogenates.


Neuroblastoma cell differentiation: a tissue culture study using time-lapse cinematography.


Description of "Minnesota Thermal Disks" and normal values of cutaneous thermal discrimination in man.


Acute hypertension and prolonged focal cerebral ischemia.


Latent herpes simplex virus infection in human trigeminal ganglia.


Reactivation of herpes simplex virus in nonpermissive cells: dependence on cell fusion.


Immunoglobulin deposits in amyotrophic lateral sclerosis.


Disorders of the immunoglobulin A system in hereditary sensory neuropathy.


The uptake of 36C1 into astrocytes in tissue culture by a potassium-dependent, saturable process: implications for epilepsy and cortical edema.


Affection of the vagus and sympathetic nerves in alcoholic polyneuropathy.


A severe pansensory familial neuropathy.


Peripheral nerve involvement in Tangier disease.


Bacterial endophthalmitis after cataract extraction. II. Incidence in 36,000 consecutive operations with special reference to preoperative topical antibiotics.


Mechanism of antibiotic-induced neuromuscular block.


Variability of neuromuscular transmission in myasthenia gravis.


Reduced acetylcholine receptors in myasthenia gravis.


Nonauditory of effects of noise.

Nonauditory of effects of noise.


Leukocytes as secretory organs of inflammation.


Herpesvirus and Epstein-Barr virus antibodies and carcinoma of the intrinsic larynx.


Hepatic cellular responses to liver cancer: abnormalities in metabolism of mitochondria isolated from human liver involved with carcinoma.


Effect of bromocarbamide on fibroblasts in vitro: fine structural changes in mouse fibroblasts.


Electron microscopy of isolated rat hepatocytes before and after treatment with phalloidin.


Necrotic changes of the rabbit adenohypophysis following orthostatic collapse.


Studies on the mode of proliferation of rat liver cells in chronic thioacetamide intoxication.


Ablation of the primary infero-temporal epileptogenic focus in rhesus monkeys with independent secondary spike discharges.


Histochemical location of glutamic dehydrogenase and aspartate aminotransferase in chicken cerebellum.


Impaired hyperpolarising inhibition during insulin hypoglycaemia and fluoroacetate poisoning.


Comparative studies on angiotensins. 3. Structure of fowl angiotensin and its identification by DNS-method.


The urinary excretion of 1-methylimidazole-4-acetic acid, a histamine metabolite, by healthy and asthmatic children.


Altered bile acid metabolism during treatment with phenobarbitone.


Triglyceride storage disease: a group of inborn errors of triglyceride metabolism.


Measurable effect (5.0--0.005 pg-ml) of ACTH acting on sections of adrenal cortex.


Left ventricular function in chronic obstructive pulmonary disease with and without cardiac failure.


Carbohydrate metabolism in patients with primary hepatocellular carcinoma.


The relationship of steroid excretions to signs of sexual maturation phenomena in normal male adolescents.


I-cell disease (mucolipidosis type II). Serum hydrolases in obligate heterozygotes.


Non-cytopathic, non-productive infection by herpes simplex viruses types 1 and 2.


Transformation of baboon cells with feline sarcoma virus.


Effect of nonspecific factors on focus formation by Rous sarcoma virus. II. Polyanions, divalent cations, temperature, and tryptose phosphate broth.


Silica urolithiasis in laboratory dogs fed semisynthetic diets.


Effect of sequential inoculations of Sendai virus and Pasteurella pneumotropica in mice.


Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay.


Spin-labeled sulfonyl fluorides as active site probes of protease structure. I. Comparison of the active site environments in alpha-chymotrypsin and trypsin.


Enzymatic synthesis of phosphoribosylamine in human cells.


Spin-labeled sulfonyl fluorides as active site probes of protease structure. II. Spin label syntheses and enzyme inhibition.


Kinetic properties of allosteric adenosine monophosphate nucleosidase from Azotobacter vinelandii.


Propagation in vitro of functional rat adrenal cortical cells: modifications of the differentiated state by culture conditions.


On the question of the existence of a guanosine triphosphate-specific protein kinase activated by adenosine 3':5'-monophosphate-dependent protein kinase.


The effect of isoproterenol upon the activity and intracellular distribution of pyrimidine nucleoside kinases in the mouse parotid gland.


Binding of bacitracin to cells and protoplasts of Micrococcus lysodeikticus.


Kinetic studies on the reaction between apocytochrome c peroxidase and protoporphyrin IX.


The structures of oligosaccharides accumulating in the liver of G-M1-gangliosidosis, type I.


Action of magnesium ion on diphosphopyridine nucleotide-linked isocitrate dehydrogenase from bovine heart. Characterization of the forms of the substrate and the modifier of the reaction.


The molecular mechanism of the in vitro 4 S to 5 S transformation of the uterine estrogen receptor.


Adenylate kinase in human tissue. I. Organ specificity of adenylate kinase isoenzymes.


Structure of the carbohydrate units of IgE immunoglobulin. I. Over-all composition, glycopeptide isolation, and structure of the high mannose oligosaccharide unit.


Structure of the carbohydrate units of IgE immunoglobulin. II. Sequence of the sialic acid-containing glycopeptides.


Inhibition by dithiothreitol of the utilization of glutamine by carbamyl phosphate synthetase. Evidence for formation of hydrogen peroxide.


The Trp-59 fluorescence of ferricytochrome c as a sensitive measure of the over-all protein conformation.


Electron paramagnetic resonance-detectable electron acceptors in beef heart mitochondria. Ubihydroquinone-cytochrome c reductase segment of the electron transfer system and complex mitochondrial fragments.


Phosphatase activity of some Mycoplasma species.


Bactericidal effect of antibiotics on Haemophilus influenzae.


Hospital treatment of alcoholism. A new therapeutic concept.


The biochemistry of alcohol.

The biochemistry of alcohol.


Application of antimony trichloride reagent in biochemical and chemotaxonomic studies relating to diosgenin and yamogenin in plants.


Alteration of ethanol-induced CNS depression: ineffectiveness of drugs that modify cholinergic transmission.


Coordinating mental health systems: a national and international perspective.


Kinetics and the free-radical mechanisms of tumor growth.


Herpes simplex infection of HEp-2 and L-929 cells. 2. The kinetics of virus multiplication and cell lysis, and the measurement of interferon production.


Free radical induced in rat liver by a chemical carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine.


The effect of chloroform, phenols, alcohols and cyanogen iodide on the swelling of Pseudomonas aeruginosa in various salts.


ESR signals during x-irradiation of tissue: their characteristics and relationship to the cancerous state.


Nuclear magnetic resonance as a new tool in cancer research: human tumors by NMR.


Experiences with ESR as a tool for surveying narcotic addiction.


Paramagnetic species and radical products in cat spinal cord.


The effect of ambient temperature on spin-label immunoassay for morphine.


Structural studies of transition state analog complexes of creatine kinase.


The use of spin labels for measuring distances in biological systems.


Magnetic resonance studies of human hemoglobins and their implications to the structure-function relationships in human normal and abnormal hemoglobins.


Ligand-enzyme interactions and enzyme regulation.


NMR relaxation studies of the unfolding and refolding of staphylococcal nuclease at low pH.


Cytochrome c-nucleotide complexes and the role of unpaired electrons in coupling processes.


EPR and other studies of the anion-binding sites of transferrin.


Ionic free radicals induced by ionizing radiation in oriented DNA.


Effects of metal ions on radical formation in gamma-irradiated DNA in the solid state.


ESR and ENDOR studies of irradiated nucleic acid constituents.


Structure and formation of free radicals in irradiated single crystals of nucleic acid constituents.


Radiation-induced radicals in thymine and its derivatives in low temperature glasses.


Long-lived radiation-induced free radicals in anoxic wet DNA as studied by electron spin resonance spectroscopy.


Equality of the rates of lateral diffusion of phosphatidylethanolamine and phosphatidylcholine spin labels in rabbit sarcoplasmic reticulum.


The noncovalent coupling of rhodopsin and phosphatidylcholines.


Electron spin resonance studies with the volatile anesthetics on phospholipid model membranes.


Boundary lipid and fluid bilayer regions in cytochrome oxidase model membranes.


The measurement of zero field splitting and the determination of ligand composition in mononuclear nonheme iron proteins.


Triphosphate spin-label studies of allosteric interactions in hemoglobin.


The use of spin labels in the study of muscle proteins.


Molecular architecture of bilayer membranes.


Carbon-13 NMR studies of peptide hormones and their components.


Spin-probe analysis of membrane perturbations produced by chemical and physical agents.


Electron spin resonance in silkworm eggs.


ESR studies of radiation damage in nucleic acids, bases, nucleosides and nucleotides.


Heme-spin-label studies on human hemoglobin.


New EPR methods for the study of very slow motion: application to spin-labeled hemoglobin.


Anomalous relaxation of water protons in solutions of copper-containing proteins.


Carbon-13 Fourier transform NMR studies in 20 mm sample tubes: observation of individual carbon sites in proteins.


ESR and NMR studies with deuterated flavodoxin.


EPR studies of iron-sulphur proteins of plant mitochondria.


Identification of primary photosynthetic processes.


Primary processes in chloroplast photosynthesis: EPR studies of bound ferredoxin and P700.


The role of plastocyanin in the photosynthetic electron transport chain as studied by EPR spectroscopy.


Electron nuclear double resonance (ENDOR) investigation on myoglobin and hemoglobin.


A fast kinetic component of ESR signal II in photosynthesis.


Isolated growth hormone deficiency. Two families with autosomal dominant inheritance.


Congenital adrenal hyperplasia.

Congenital adrenal hyperplasia.


Proceedings: Uses of a routine postmortem radiography.


Growth and skeletal maturation in congenital adrenal hyperplasia. Review of 20 cases.


Diagnosis and clinical significance of parainfluenza virus infections in children.


Conformational analysis of a cyclic nucleotide: 3'-5'-adenosine monophosphate. A study based on the extended Hückel theory.


Multiple forms of protein kinase in liver cell. I. Cytosol.


Molecular mobility of polypeptides containing proline as determined by 13C magnetic resonance.


Leiomyoma. An unusual tumour of the orbit.


Diseases of the skin. Treatment of scalp disorders.


Protein-binding assay for plasma testosterone, after purification by column partition chromatography.


Diseases of the skin. Present and future trends in approaches to skin disease.


Advantages and disadvantages of nephelometric measurement of serum lipids.


Directional effects of cyclic AMP on platelets.


The effect of fetal pituitary ACTH and maternal corticosteroid on the development of fetal rat adrenal cortex.


Metabolism of mammalian sympathetic ganglia.


Role of cyclic AMP in synaptic transmission in the mammalian peripheral nervous system.


Physiological significance of protein degradation in animal and bacterial cells.


Ribosomal RNA cistron multiplicity and nucleolar organizers in hexaploid wheat. The ribosomal DNA (DNA complementary to ribosomal RNA) content of twelve different wheat genotypes has been studied. Some of these genotypes are aneuploids with deletions or additions of chromosomes bearing nucleolar organisers. The rDNA contents of these genotypes provide several examples of a clear departure from a correlation between the number of rRNA cistrons and the number of nucleolar organisers. Thus the number of rRNA cistrons per nucleolar organiser is not constant in wheat. Wheat DNA was found to have a mean buoyant density of approximately 1.702 g/cc for all genotypes studied and rRNA hybridized selectively to DNA of buoyant density approximately 1.710 g/cc. The thermal stabilities of all the rRNA/DNA hybrids were essentially identical.


Nineteen paracentric chromosomal inversions in mice. Using mutagens on sperm and spermatids we have produced nineteen chromosomal inversions in mice. The levels of radiation and chemical mutagen we used induced inversions in about one per cent of the animals screened. Nine inversions have been transmitted through successive generations, and the particular frequency of first meiotic anaphase bridges manifested by each inversion remained constant. The cytological properties of first meiotic anaphases varied considerably among the inversions. Chromosomal locations of five inversions are known. Four of the five are fully viable in homozygous condition.


Translocations, the predominant cause of total sterility in sons of mice treated with mutagens. Histological and cytological analyses of the testes were carried out in 42 sterile sons of males treated in the spermatozoal or spermatid stage with 250 mg/kg ethyl methanesulfonate (EMS) alone or after prefeeding with butylated hydroxytoluene (BHT); or treated with 200 R X-rays. Of the 42 sterile males, 17 had some mature spermatids, nine were blocked at diakinesis, 15 were blocked in pachytene, and one lacked spermatogenic cells altogether, having Sertoli cells only. Mitotic (spermatogonial) metaphases could therefore be analyzed in 41 of the males and meiotic configurations in 26.-(1) None of the males showed abnormalities in chromosome number, such as monosomy, trisomy, or mosaicism for either of these conditions. Certain classes of chromosome abnormalities that have been found associated with male sterility in other investigations, namely trisomies, XXY's, and X-autosome translocations, are not expected from treatment of 19A + Y cells when F(1) males are studied. (2) A very high percentage of the sterile males carried translocations. Direct meiotic evidence for this was found in 22 of the animals. In addition, 11 of the 16 that were blocked (or virtually blocked) in pachytene, and thus could be analyzed in mitosis only, consistently showed one abnormally short chromosome (or, one short plus one long), which presumably had resulted from unequal exchange (or sizable deficiency). Of the meiotically detected translocation males, 1 carried a T(A;Y), 17 had single autosomal translocations, and 4 had multiple autosomal rearrangements involving three, four, four, and six breaks, respectively. In addition, three males showed failure of X-Y pairing. (3) Translocations that cause sterility, rather than partial sterility, in males appear to be those in which at least one of the breaks occurs close to one end of a chromosome. The mitotic and meiotic evidences for this were found to be correlated. (4) It is proposed that many cases of induced F(1) male sterility may be the result of position effects produced when paracentromeric regions are translocated to euchromatic regions of certain other chromosomes. Since many translocations that produce partial sterility in the female cause complete sterility in the male, the male must be assumed to be more susceptible to disturbances of fertility by the postulated mechanism. (5) There is evidence that EMS, especially in the lower dose range, more often breaks chromosomes near one of their ends than does X-irradiation.


Studies on the mechanisms of invasion in cancer. 3. Purification of a neutral protease of rat ascites hepatoma cell associated with production of chemotactic factor for cancer cells.


Potentiation of RSV rescue from RSV-transformed "poorly" virogenic cell lines by 5-bromodeoxyuridine treatment before fusion with chick embryo fibroblasts.


Immunogenicity of embryonic antigens associated with chemically induced rat tumours.


Further studies on the ability of regressor sera to block cell-mediated destruction of Rous sarcomas.


Antigenic relatedness of simian C-type viruses.


Sensitivity to guinea-pig serum of hamster cells transformed in vitro by two strains of the Rous sarcoma virus.


Non-specific serum inhibitors of activity of haemagglutinins of rabies and vesicular stomatitis viruses.


Interfering particles from a culture persistently infected with Parana virus.


The effect of canavanine on herpes simplex virus replication.


Lipid and protein organization in vesicular stomatitis and Sindbis viruses.


Imaging functional nodules of the adrenal glands with 131-I-19-iodocholesterol.


Scintiscanning of pulmonary diseases with 67Ga-citrate.


The toxicity of Sn-pyrophosphate: clinical manifestations prior to acute LD50.


99mTc-pyrophosphate for bone imaging.

99mTc-pyrophosphate for bone imaging.


Sella turcica salivary transplants in hypophysectomized animals.


Effect of exogenous and endogenous glucocorticosteroids on the in vitro stimulation of lymphocytes from sedated and awake-restrained healthy baboons.


Supercoiled DNA: hydrodynamic model.

Supercoiled DNA: hydrodynamic model.


Cholesterol and cell membrane function: a hypothesis concerning etiology of atherosclerosis.


Letter: On the inclusion of spontaneous emission in a neuronic equation of state.


An administrative record system for ambulatory facilities.


Rickets, American style.

Rickets, American style.


Control of cell division by very lysine rich histone (F1) phosphorylation.


Pressor effect of angiotensin II in sodium replete and deplete rats.


Primary hyperparathyroidism and peptic ulcer disease.


The presence of radioimmunoassay renin levels in anephric man.


Control of and by pH.

Control of and by pH.


Herpes simplex infection of HEp-2 and L-929 cells. 4. The effect of lysosomal inhibitors.


The action of heat, chemicals and disinfectants on swine vesicular disease virus.


Axon retraction following guanethidine treatment. Studies of sympathetic neurons in vivo.


Further observations on a cytoplasmic structure in the hamster oocyte.


Electron microscopic study of rat pituitary primordium in organ culture.


Fine structural observations of the pecten oculi capillaries of the chicken. Freeze-etching, scanning and transmission electron microscopic investigations.


Sizes of granular vesicles in the rat median eminence, with special reference to the zona granulosa.


Absorption of protein macromolecules by the enterocytes of the carp (Cyprinus carpio L.). Ultrastructural and cytochemical study.


The structure of the salivary gland of the moth (Manduca sexta).


The proteins of plasma lipoproteins: properties and significance.


Peripheral neuropathy presenting with respiratory insufficiency as the primary complaint. Problem of recognizing alveolar hypoventilation due to neuromuscular disorders.


Fatal disseminated herpesvirus hominis type 2 infection in an adult with associated thymic dysplasia.


Adult meningoencephalitis caused by herpesvirus hominis type 2.


Chromosomal analysis in malignant ovarian tumors.


Lipoprotein deficiency disorders.

Lipoprotein deficiency disorders.


The synthesis of matrix components by chondrocytes in vitro in the presence of 5-bromodeoxyuridine.


Type II hyperlipoproteinaemia.

Type II hyperlipoproteinaemia.


Tentative method of analysis for cadmium content of atmospheric particulate matter by atomic absorption spectroscopy.


Tentative method of analysis for lead content of atmospheric particulate matter by atomic absorption spectroscopy.


Tentative method of analysis for iron content of atmospheric particulate matter by atomic absorption spectroscopy.


Tentative method of analysis for manganese content of atmospheric particulate matter by atomic absorption spectroscopy.


Fixation of manganese on quartz, limestone, dolomite and marine sediment samples.


Tentative method of analysis for zinc content of atmospheric particulate matter by atomic absorption spectroscopy.


Lung cancer among uranium miners in the United States.


An evaluation of the 20 minute perchlorate discharge test.


A simplified radioimmunoassay of plasma aldosterone.


Effect of various ACTH preparations and of metyrapone on the secretion of growth hormone in normal subjects and in hypopituitary patients.


The nocturnal rise of human prolactin is dependent on sleep. Further evidence that there is a relationship between prolactin rise and sleep is offered by this study that delayed sleep onset and inverted the normal sleep-waking cycle in 4 normal patients (3 women and 1 man).  The inversion schedule lasted 3 weeks, and at its conclusion a final 12-hour laboratory study was carried out measuring the plasma levels of human prolactin and growth hormone by radioimmunoassay.  In the first female subject, mean prolactin levels (ng/ml) were, for baseline and after shift, 7 and 8.3; for case 2 (female) the values were 7.3 and 5.9; for case 3 (male) the levels were 6.1 and 6.1; and for the last case, the levels were 4.7 and 4.3, respectively.  Values for growth hormone were (ng/ml) 1.5 and 1.5 for Case 1 for baseline and after shift, respectively; 1.1 and 2.5 for Case 2, respectively; 1.8 and 1.1 for Case 3, respectively; and 7.3 and 5.2 for Case 4, respectively.  Prolactin release shifted immediately and completely with shifts of sleep onset of 3, 6, and 12 hours.  Thus, it is concluded that the nocturnal rise is dependent on the occurrence of sleep, not on the inherent rhythm related to time of day.


The urinary excretion of adenosine 3',5'-monophosphate in children and adolescents.


Detection of specific surface antigens on cells transformed by cytomegalovirus with the techniques of mixed hemagglutination and 125I-labeled antiglobulin.


Immunity to murine sarcoma virus-inducted tumors. I. Specific T lymphocytes active in macrophage migration inhibition and lymphocyte transformation.


Immunity to murine sarcoma virus-inducted tumors. II. Suppression of T cell-mediated immunity by cells from progressor animals.


Phagocytosis: a defense mechanism against infection with Trypanosoma cruzi.


Periodate-induced lymphocyte transformation. IV. Mitogenic effect of NaIO4 treated lymphocytes upon autologous lymphocytes.


Reversal of tumor-mediated suppression of immune reactivity by in vitro incubation of spleen cells.


A comparative study of the ultrastructure of the sebaceous glands of man and other primates.


Hormonal control and methods of measuring sebaceous gland activity.


The control and measurement of sebum secretion.


Endocrinologic control of the development and activity of the human sebaceous gland.


Receptor proteins for androgen in hamster sebaceous glands.


Inhibition of dihydrotestosterone formation: an effective means of blocking androgen action in hamster sebaceous gland.


Ultrastructural observations in acne vulgaris: the normal sebaceous follicle and acne lesions.


Proceedings: Reinnervation of skeletal muscle in the mouse.


Cancer of the lung in young men.

Cancer of the lung in young men.


Dual action of praseodymium (Pr3+) on transmitter release at the frog neuromuscular synapse.


Sensitivity to amphotericin B of in vitro established cell lines.


Morphine-like drugs inhibit the stimulation of E prostaglandins of cyclic AMP formation by rat brain homogenate.


Interaction of mercurials with salmon serum lipoproteins.


Inhibitor of vesicular stomatitis virus transcriptase in purified virions.


Methionine transfer RNAs associated with avian oncornavirus 70S RNA.


Growth hormone releasing factor of microbial origin.


Light-regulated guanosine 3',5'-monophosphate phosphodiesterase of bovine retina.


Similarity in the sequence of Escherichia coli dihydrofolate reductase with other pyridine nucleotide-requiring enzymes.


Augmentation of cytotoxic drug action by antibodies directed at cell surface.


Quantitative aspects of interferon-induced plaque reduction: effects of cell age and dose of challenge virus.


Studies on the anterior pituitary hormones of the kangaroo.


Antibodies to viruses of human origin in monkeys from Uganda.


Oral induction of interferon by a low-molecular-weight compound.


Reciprocal relationship between the in vitro synthesis and secretion of prolactin and growth hormone.


Studies on the prevalence of endogenous type C virus RD 114 in cats.


Comparison of the ribonucleotide with the canavanine reductase system.


The inhibition of virus adsorption by substances induced by DEAE-dextran and poly I:C.


The response of glucose-6-phosphatase in human and rat fetal liver cultures to dibutyryl cyclic AMP.


Effect of the inhibition of angiotensin I converting enzyme in endotoxin and hemorrhagic shock.


The conjugation of bilirubin by rat hepatoma cells in tissue culture.


Fanconi's anaemia.

Fanconi's anaemia.


Stimulation and suppression of erythropoiesis in the plethoric mouse by cyclic nucleotides.


Dietary factors affecting the pathogenicity of Entamoeba histolytica in rats.


Glioblastoma multiforme of the spinal cord.


In vitro activity of metronidazole on Entamoeba histolytica in axenic culture.


Proceedings: The interaction between E. histolytica and rabbit kidney cell (RK13) monolayers.


Proceedings: Rhabdo-virus like particles of Entamoeba histolytica.


Study of Naegleria aerobia by electron microscopy.


Properties of the polyoma virus transcription complex obtained from mouse nuclei.


Recombination between temperature-sensitive mutants of simian virus 40.


C-type particles produced by a permanent cell line from a leukemic pig. I. Origin and properties of the host cells and some evidence for the occurrence of C-type-like particles.


Endonucleolytic DNase in oncorna viruses: role of mycoplasma.


A comparison of the high molecular weight RNAs of visna virus and Rous sarcoma virus.


Pseudotypes of vesicular stomatitis virus determined by exogenous and endogenous avian RNA tumor viruses.


Membrane alterations which accompany MuMTV maturation. I. Studies by freeze-cleave techniques.


Stimulation of fetal thymidine kinase in cultured human fibroblasts transformed by SV40 virus.


Enhancing factor for Rous sarcoma virus: in vitro assay and action on both cells and virus.


Accumulation of closed-circular polyoma DNA molecules containing host DNA during serial passage of the virus.


Extravesical malakoplakia.

Extravesical malakoplakia.


Herpesvirus varicellae isolated from human dorsal root ganglia.


Chemotherapy of breast cancer. Investigational treatment.


Agents which block the action of the renin-angiotensin system.


Senescence and the renal vasculature in normal man.


Inhibition by acetylcholine of adrenergic neurotransmission in vascular smooth muscle.


Effect of synthetic LH-releasing factor (LRF) on LH secretion in monolayer cultures of the anterior pituitary cells of cynomolgus monkeys.


A specific progesterone receptor in the hamster uterus: physiologic properties and regulation during the estrous cycle.


Stimulation of 14C-cyclic AMP accumulation by FSH and LH in testis from mature and immature rats.


Hypophyseal-adrenocortical role in urinary excretion of epinephrine and norepinephrine in hypophysectomized and adrenalectomized rats.


Action of a nonsteroidal antiandrogen, flutamide, on the receptor binding and nuclear retention of 5 alpha-dihydrotestosterone in rat ventral prostate.


Effects of colchicine on the formation of thyroid hormone.


Evidence for different angiotensin II receptors in rat adrenal glomerulosa and rabbit vascular smooth muscle cells. Studies with competitive antagonists.


Acute hormonal regulation of cyclic AMP content and glycogen phosphorylase activity in fetal liver in organ culture.


Rat placental delta-5-3 beta-hydroxysteroid dehydrogenase. I. Changes in activity with dilution and gestational age.


General presence of glucocorticoid receptors in mammalian tissues.


Response of somatosensory cerebral neurons to stimulation of dorsal and dorsolateral spinal funiculi.


Effects of projected cortical epileptiform discharges on unit activity in the cat cuneate nucleus.


The receptive surface and axonal terminals in severely denervated neurons within the lumbosacral cord of the dog.


The development of unit activity in the lateral geniculate nucleus of the kitten.


Effects of projected cortical epileptiform discharges on field potentials in the cat cuneate nucleus.


Ontogenesis of ponto-geniculo-occipital activity in the lateral geniculate nucleus of the kitten.


Electrical and chemosensitive properties of normal and dystrophic chicken muscles.


Adenine arabinoside effect on experimental idoxuridine-resistant herpes simplex infection.


Role of Ca2+ in adrenocorticotropic hormone-stimulated lipolysis in the perfused fat cell system.


Ascorbate oxidase. Inhibition, activation, and pH effects.


Activation of succinate dehydrogenase by anions and pH.


Lipogenesis in rat hepatocytes.

Lipogenesis in rat hepatocytes.


Studies on human beta-D-N-acetylhexosaminidases. I. Purification and properties.


Interaction of fragment A from diphtheria toxin with nicotinamide adenine dinucleotide.


Glucocorticoid receptors in mouse mammary tumors. Specific binding of glucocorticoids in the cytoplasm.


Studies on cobalt myoglobins and hemoglobins. 3. Electron paramagnetic resonance studies of reversible oxygenation of cobalt myoglobins and hemoglobins.


Univalent reduction of molecular oxygen by spinach chloroplasts on illumination.


Effect of insulin and lipolytic agents on rat adipocyte low Km cyclic adenosine 3':5'-monophosphate phosphodiesterase.


Characterization and solubilization of gonadotropin receptor of bovine corpus luteum.


Purification and characterization of lipoprotein lipase from pig adipose tissue.


Metalloflavoprotein models. The crystal structure of bis(riboflavin) bis(cupric perchlorate) dodecahydrate.


Quantitative aspects of the insulin-receptor interaction in liver plasma membranes.


Biochemical studies on the mode of action of cytochalasin B. Preparation of (3H)cytochalasin B and studies on its binding of cells.


A kinetic study of the complementation of fragments of staphylococcal nuclease.


Characterization of cardiac muscle collagen. Molecular heterogeneity.


Membrane-bound angiotensin-converting enzyme from rat lung.


Multiple disc gel bands of mitochondrial creatine and adenylate kinases.


Phosphoinositide metabolism in rat superior cervical ganglion, vagus and phrenic nerve: effects of electrical stimulation and various blocking agents.


Synaptosomal plasma membranes: acyl group composition of phosphoglycerides and (Na+ plus K+)-ATPase activity during fatty acid deficiency.


Effect of behavioural training on brain transfer RNA methylases.


Effects of levorphanol on phospholipid metabolism in the giant axon of the squid.


Melanoameloblastoma.

Melanoameloblastoma.


Cyclic nucleotide phosphodiesterase of the bovine retina: activity, subcellular distribution and kinetic parameters.


Calcium metabolism in isolated brain cells and subcellular fractions.


Editorial: The gene for the poliovirus receptor.


Pathogenesis of experimental viral hepatitis (second of two parts).


ACTH and the regulation of adrenocortical secretion.


Possible involvement of cyclic GMP in growth control of cultured mouse cells.


Dramatic early increase in uterine eosinophils after oestrogen administration.


Thymine-labelled deoxyoligonucleotide involved in DNA chain growth in Bacillus subtilis.


Protease inhibitors do not block transformed cells in the G1 phase of the cell cycle.


Viroids and viral hepatitis in marmosets.


Detection by DNA polymerase I of breaks produced in rat liver chromatin in vivo by alklating agents.


Community psychiatry at UCLA: a decade of training.


Effects of cyclopropane on contractility and the cyclic 3',5'-adenosine monophosphate system in the rat aorta.


Oral medicine for the dental practitioner. 2. Infections of the oral mucosa.


The epidermis and cyclic AMP.

The epidermis and cyclic AMP.


Proceedings: Giant glomangioma.

Proceedings: Giant glomangioma.


Editorial: Unsuspected cytomegalic mononucleosis.


Cure of insulin-dependent diabetes mellitus by removal of a glucagonoma.


The effect of pancuronium on reflex regulation of heart rate in man.


Lack of thrombohemorrhagic phenomena complicating childhood solid tumors.


Pemphigus vulgaris and internal malignancy.


Cerebral response to hypocapnia in normal and brain-injured dogs.


Herpes esophagitis complicating Hodgkin's disease.


A survival study of intestinal and diffuse types of gastric carcinoma.


The treatment of malignant pleural effusions.


Differential diagnosis of breast tumors. The sensitivity graded method ultrasonotomography and clinical evaluation of its diagnostic accuracy.


Secretory otitis media: protein and enzyme analyses.


Serum alpha-fetoprotein in 184 Ugandan patients with hepatocellular carcinoma. Clinical, laboratory, and histopathologic correlations.


A note on differentiation and divisibility of alveolar epithelial cells.


Scanning electronmicroscopy of virus-infected respiratory epithelium.


Morphologic and ultrastructural changes in the lungs of animals during acute exposure to ozone.


Fibrous histiocytoma of the lung (sclerosing hemangioma variant?).


Myopathies in the elderly.

Myopathies in the elderly.


Pro-hormones in tissues and in circulation.


Studies on the pituitary "Fellstoffwechselhormon". V. The further purification of lipolytic active peptides from hog pituitary glands.


Inhibitory effects of calcitonin on lipolysis and 47 calcium accumulation in rat adipose tissue in vivo.


Regulation of adenylate cyclase and cyclic-AMP dependent protein kinase activities in A2-cell rich guinea-pig islets of Langerhans.


Effect of irreversible adsorbents on the dissociation of the antigen-antibody complexes.


Effect of cyclic AMP on ketogenesis in liver homogenates from pancreatectomized-hypophysectomized dogs.


Human medullary thyroid carcinoma: control of calcitonin secretion in vivo and in tissue culture.


Population genetic studies of red cell enzyme polymorphisms in four Spansih populations.


Immunology of Ascaris suum infection. I. Production of reaginic antibodies to worm components in rats.


Heterogeneity of antigen from callus of man.


Operative culdoscopy ad treatment for the polycystic ovary.


Artificial insemination. Induced ovulation with intravenous estrogens.


Bladder dysfunction: a sign of occult colonic carcinoma.


Endocavitary irradiation in the curative treatment of early rectal cancers.


Toxic action of anti-H.C.G. antibody to human trophoblast.


Improved methodology for scanning electron microscopy of the female reproductive tract.


Autoantibody formation against spermatozoa caused by extravasation of spermatozoa into the interstitium of the epididymis of aged men.


Etiology and treatment of failed pregnancies.


Occurrence of embryo mortality in rabbits isoimmunized against semen.


Preliminary efforts to capacitate rabbit sperm in vitro.


Ovarian receptivity to human gonadotropins in primary selective pituitary deficiency in gonadotropins.


Evidence for discontinuous replication of circular mitochondrial DNA molecules from Novikoff rat ascites hepatoma cells. Double-forked circular molecules of mitochondrial DNA (mtDNA) from rat tissues, indicated by their form and size to be replicative intermediates, are of two structurally distinct classes. Molecules of the first class are totally double stranded. Molecules of the second class are defined by one daughter segment being totally or partially single stranded. Length histograms of daughter segments measuring between 2% and 44% of the total 5-microm molecular contour were constructed from samples of both classes of replicating molecules derived from mtDNA or Novikoff rat ascites hepatoma cells. For single strand-containing molecules, the lengths fell into eight distinct, reproducible groups with mean values separated by 4.1-7.6% of the circular contour length. For totally double stranded molecules, the lengths fell into seven groups, corresponding to seven of the groups found for single strand-containing molecules. These results suggest that along at least 44% of the contour of mtDNA molecules there exist discrete points at which DNA synthesis tends to be arrested. This may indicate that there are pauses in normal mtDNA synthesis. However, as the DNA used in these experiments was isolated from mitochondrial fractions, the findings may indicate that continuation of synthesis beyond specific points on the nucleotide strands requires a factor which is not available after cell disruption.


Fusion of erythrocytes and other cells with retention of erythrocyte cytoplasm: nuclear activation in chicken erythrocyte-melanoma heterokaryons.


Ultrastructural localization of cytochrome b5 on rat liver microsomes by means of hybrid antibodies labeled with ferritin.


Structural analysis of phosphatidylcholine of plant tissue. Pure preparations of phosphatidylcholine were isolated from spinach leaf chloroplasts, spinach leaf microsomes, and cauliflower inflorescence. The isolated phosphatidylcholine was treated with snake venom phospholipase A, and the fatty acid distribution and composition of the fatty acid methyl esters prepared from the lysophosphatidylcholine and the freed fatty acid were determined by gas-liquid chromatography. The results showed that saturated fatty acids were preferentially esterified at position 1 and unsaturated fatty acids at position 2. The phosphatidylcholine from cauliflower was also treated with phospholipase C. The resulting diglycerides were fractionated on AgNO(3)-impregnated thin-layer plates. The diglyceride fractions were transesterified and the fatty acid composition of each was determined by gas-liquid chromatography. The predominant species contained linolenic acid only (22% of the total), linolenic and oleic acids (19%), and linolenic and palmitic acids (37%). These molecular species could not be accounted for by random distribution of the fatty acids.


Biosynthesis of phosphatidylcholine by enzyme preparations from spinach leaves. The enzymic incorporation of choline-1,2-(14)C from CDP-choline-1,2-(14)C into phosphatidylcholine by spinach leaf preparations was characterized. The enzyme catalyzing the incorporation, choline phosphotransferase, had a pH optimum of about 8.0 and required either Mn(2+) or Mg(2+) as cofactor. The saturation concentration of Mn(2+) was 0.3 mm and that for Mg(2+) was 13 mm. The K(m) for CDP-choline was 10 micro m. The choline phosphotransferase was inhibited by sulfhydryl reagents. The enzyme was inactivated at 30 degrees C, but this inactivation could be prevented by dithiothreitol and Mn(2+). Preincubation of the enzyme with Mn(2+) prevented inhibition by sulfhydryl reagents. The incorporation of diglyceride-U-(14)C into phosphatidylcholine was also studied. The enzyme did not show any diglyceride specificity when exogenous diglyceride was added, indicating that fatty acid distribution in phosphatidylcholine of spinach is not controlled by choline phosphotransferase.


Serum lipoprotein accumulation in the livers of orotic acid-fed rats. This study provides confirmation of previous observations that showed that rats fed a diet containing 1% orotic acid for 7 days develop a fatty liver and that there is an inhibition of the secretion of low density lipoproteins without altering general liver protein synthesis. Accumulated fat droplets (liposomes) are entrapped within rough endoplasmic reticulum vesicles. In this study, these vesicles have been shown to accumulate the apolipoproteins of low and very low density lipoproteins. Inhibition of lipoprotein secretion was demonstrated by perfusion of livers from orotic acid-fed rats with a serum-free medium. Liposomes were isolated from these rats. Partially delipidated liposomes, but not similarly treated microsomes or cell sap, were found to form a precipitation line when reacted against anti-low density lipoprotein antiserum. Detergent solubilization of the liposome followed by density gradient centrifugation resulted in a peak at d 1.025 g/ml containing both lipid and protein. Acrylamide electrophoresis in 8 m urea after total delipidation demonstrated liposomal bands which coelectrophoresed with three of four very low density lipoprotein bands; there was no band corresponding to the very low density lipoprotein band which travels furthest in acrylamide electrophoregrams. However, acrylamide electrophoresis of the apoproteins of serum high density lipoprotein from orotic acid-fed animals revealed the presence of the latter band. The results indicate that liver liposomes from orotic acid-fed rats apparently contain the low density apoprotein and probably several other very low density lipoprotein peptides.


A new chromatographic system for vitamin D3 and its metabolites: resoluation of a new vitamin D3 metabolite. A simple yet powerful new chromatographic procedure for vitamin D(3) and its metabolites is described. Liquid-gel partition chromatography on Sephadex LH-20 using a solvent of various percentages of CHCl(3) in Skellysolve B (petroleum ether, bp 67-69 degrees C) permits excellent resolution of vitamin D(3), 25-hydroxyvitamin D(3), and their more polar metabolites. Of special importance is the resolution of the metabolites of vitamin D(3) more polar than 25-hydroxycholecalciferol. Because of this resolution, a new metabolite of vitamin D(3) has been demonstrated in the plasma of rats and in the intestines of chicks given 100 IU of vitamin D(3)-1,2-(3)H.


Effect of dietary tomatine on cholesterol metabolism in the rat. Tomatine is a virtually nonabsorbable saponin which has been used as an antifungal agent and analytically as a cholesterol precipitant. It was used in this study to determine whether or not it can form a complex with cholesterol in vivo in the rat intestine and what effects such complex formation would have on cholesterol metabolism. Rats that were fed tomatine as 1% of the diet had a decreased uptake of dietary cholesterol by the liver, an increased rate of hepatic and intestinal cholesterol synthesis as well as a partial offsetting of the dietary cholesterol-induced decrease in hepatic cholesterogenesis, and an apparent increase in sterol excretion without an effect on bile acid excretion. In vitro, tomatine did not sequester cholic acid as did cholestyramine. The results show that tomatine has an effect on cholesterol absorption and on other aspects of lipid metabolism in the rat similar to that of cholestyramine, with the notable exception that tomatine increased sterol excretion while cholestyramine increased bile acid excretion. It was suggested that tomatine forms a nonabsorbable complex with cholesterol in the rat intestine.


Synthesis of plasma triglycerides in endogenous hypertriglyceridemia. Radioisotopic kinetic studies of triglyceride fatty acid synthesis from serum free fatty acids have been performed in 20 studies of normal and lipemic subjects. The lipemic subjects were characterized as having carbohydrate-responsive endogenous lipemia, and were classified as having either Type III or Type IV prebetalipoproteinemia. In the untreated state, triglyceride production was reduced relative to concentration of triglyceride when compared with the normal control population. In response to carbohydrate restriction an absolute reduction in triglyceride synthesis from free fatty acids was demonstrated. These data indicate that overproduction cannot be importantly implicated as the etiology of this form of endogenous lipemia. The patients thus represent a pathophysiological entity which is distinct from the normal physiological lipemia induced by carbohydrate feeding in which overproduction is reported to be the initiating event.


Lipolytic response and adenyl cyclase activity of rat adipocytes as related to cell size. The number of fat cells contained in the rat epididymal fat pad was found to increase rapidly as the rats grew to a weight of about 300 g. Additional increases in cell number above this weight were minimal. By contrast, cell size, as measured by the amount of triglyceride per cell, increased linearly until the rats reached about 600 g. Glycerol release per 10(6) cells in response to norepinephrine in vitro was observed to be independent of cell size. Basal release expressed in this manner showed a slight but significant positive correlation with increasing cell size. When the rate of lipolysis was based either on the amount of triglyceride in the incubation medium, as is the usual custom, or on the cell surface area, lipolysis was inversely related to cell size. In addition to these observations on lipolysis, it was also demonstrated that norepinephrine-activated adenyl cyclase activity expressed per 10(6) cells was unaffected by cell size. This leads to the suggestion that the number of adrenergic receptors in the fat cell is fixed and is independent of the size of the cell; as the cell enlarges, these receptors are merely distributed over a greater surface area.


Chromatography of unusual lipids on thin layers of magnesium oxide. The chromatographic behavior of some minor components of natural lipids was studied using layers of magnesium oxide and of commercial adsorbents containing magnesium oxide. The lipids investigated included wax esters, cholesteryl esters, diester waxes, and esters, ethers and ether-esters of ethanediol and glycerol. Pronounced differences were found in the patterns of separation of certain lipids on magnesium oxide as compared to silica gel and Florisil. The procedures described afford means for the detection of unusual lipids in natural materials, and their isolation.


Resolution of intact phosphatidylinositols by argentation thin-layer chromatography. An argentation thin-layer chromatographic method is described for the separation of intact phosphatidylinositols on the basis of the degree of unsaturation of their component fatty acids. The system is applicable for metabolic studies using radioactive precursors of phosphatidylinositol.


Cytomegalovirus mononucleosis.

Cytomegalovirus mononucleosis.


Proceedings: Gluconeogenesis in fetal and neonatal lambs.


Juvenile nasopharyngeal angiofibroma.

Juvenile nasopharyngeal angiofibroma.


Effect of camptothecin on simian virus 40 DNA.


Antibody response and virus survival in cats vaccinated against feline leukaemia.


Cyclic GMP response in vivo to cholinergic stimulation of gastric mucosa.


Colchicine and vinblastine inhibit fibroblast aggregation.


Efferent innervation of vestibular and auditory receptors.


Functional significance of connections of the inferior olive.


Poly I-poly C and endotoxins share immunosuppressive properties and increase the toxicity of alpha-amanitin and hexobarbital.


Frequent occurrence of hypoplastic optic disks in patients with aniridia.


The team approach to rehabilitation after mastectomy.


Letter: A serological survey of parainfluenza type 3 virus infection in pigs in Victoria.


Alcohol ingestion following intracerebral angiotensin administration and water deprivation.


Effects of angiotensin II on central neurons.


Respiratory metabolism of tissues in septal forebrain lesioned rats.


Comparative studies on the digestive physiology of two sympatric species of Oxyrhachis (Germar) (Membracoidea: Oxyrhachinae) with notes on their taxonomy.


Ovarian neoplasms in children and adolescents.


Orchioblastoma--a report of two cases.

Orchioblastoma--a report of two cases.


Mixed hepatoblastoma--a case report.

Mixed hepatoblastoma--a case report.


Microflora of fresh and dry-cured hams as affected by fresh ham storage.


Endodermal sinus tumor of ovary--a case report.


Dissociation of plasma and spinal fluid ACTH in Nelson syndrome.


Bilateral aseptic necrosis of femur following a 16-day course of corticotropin.


Hepatoma--foetal Phe-tRNA also present in normal rat liver.


Response of mouse melanoma cells to melanocyte stimulating hormone.


Stimulation of calcitonin synthesis and release in vitro by calcium and dibutyryl cyclic AMP.


Ultrastructural changes of erythrocyte membranes isolated with colloidal silica solution (Ludox).


Nocturnal secretory patterns of FSH, GH, LH and TSH.


Ultrastructural study of the pericardial organ-anterior ramifications complex neurosecretory terminals.


Ultrastructure of the sexual segments of the kidney in male and female lizards, Cnemidophorus l. lemniscatus (L.).


Development of muscle spindles deprived of fusimotor innervation.


Ultrastructural changes in the secretory cycle of parathyroid cells of winter frogs (Rana pipiens) after pituitary homoimplantation.


Electron microscopic study of the substantia nigra and the strio-nigral projection in the rat.


Surface morphology of the subfornical organ in the rabbit's brain.


Effect of cycloheximide on the fine structure of corpus luteum in intact and hypophysectomized rats.


Fine structure of capillaries in the pecten oculi of the sparrow, Passer domesticus.


Lysosomal enzymes in neurological and psychiatric conditions.


Changes in total histamine content of white blood cells (WBC) in allergy patients.


Corticosteroids, circulating leukocytes, and erythrocytes in cattle: diurnal changes and effects of bacteriologic status, stage of lactation, and milk yield on response to adrenocorticotropin.


Multiple viral infection in calves with acute bovine respiratory tract disease.


Hypolipoidemia and hypergammaglobulinemia associated with experimentally induced anaplasmosis in calves.


Growth of bovine cell monolayer cultures from tissue explants.


A gas chromatographic-mass spectrometric analysis of the esterifying alcohols of pumpkin seed protochlorophylls.


A rapid and sensitive radiometric assay procedure for thiamine pyrophosphokinase activity using anion-exchange paper discs.


Metal-ion distribution in metalloproteins by proton-induced x-ray emission analysis.


Humoral control of airways.

Humoral control of airways.


Induction of the humoral antibody response via the respiratory tract.


Synthesis of polyadenosine diphosphate ribose by isolated nuclei of swine aortic tissue.


Interaction of cytochrome c peroxidase with cytochrome c.


The Epstein-Barr Virus (EBV) in Burkitt's lymphoma and nasopharyngeal carcinoma.


Some properties of a cytochrome c-mixed mitochondrial phospholipid complex.


Biosynthesis of head-to-head terpenes. Carbonium ion rearrangements which lead to head-to-head terpenes.


Molecular basis of cell proliferation: the cell cycle.


Base-sequence relationships between avian ribonucleic acid endogenous and sarcoma viruses assayed by competitive ribonucleic acid-deoxyribonucleic acid hybridization.


The separation and partial purification of the soluble 17 alpha- and 17 beta-hydroxysteroid dehydrogenases of rabbit liver.


Synthesis of peptides with the solid phase method. II. Octapeptide analogues of angiotensin II.


Adenosine-3',5'-monophosphate-dependent protein kinase from bovine anterior pituitary gland. 3. Structural specificity of the ATP site of the catalytic subunit.


Synthesis of peptides by the solid phase method. I. Tetradecapeptide renin substrate and intermediate peptides, nonapeptides, and fragments of angiotensin II.


Metabolism and transport of purine nucleosides by membrane preparations of Micrococcus sodonensis.


Respiratory viral infections among thoroughbred horses in training during 1972.


Abnormal property of human mutant hypoxanthine-guanine phosphoribosyltransferase: insensitivity of fibroblast enzyme to stabilization against freezing and thawing by 5-phosphoribosyl-1-pyrophosphate.


Laboratory aids in the diagnosis of pituitary tumors.