Phosphatase activity of methicillin-resistant and methicillin-sensitive cells of Staphylococcus aureus.


Phospholipids and acyl groups of subcellular membrane fractions from human intracranial tumors. The phospholipids from subcellular fractions of human intracranial tumors were examined. For comparison, microsomes were isolated from a fetal human brain and from the gray matter of adult human brains. The subcellular membranes of tumors had a higher protein-to-phospholipid ratio than the normal brain membranes. The microsomes from tumors had a lower proportion of diacylglycerophosphorylethanolamine and higher proportions of alkenylacylglycerophosphorylcholine and sphingomyelin (plus diacylglycerophosphorylinositol) than microsomes from the gray matter. Also, the ratios of alkenylacylglycerophosphorylethanolamine to diacylglycerophosphorylethanolamine were higher in the tumors than in the normal controls. The acyl groups of ethanolamine phosphoglycerides in tumor microsomes had relatively more 18:1, 18:2, and 20:4(n - 6) and less 18:0, 22:4(n - 6), and 22:6(n - 3) than the adult brain gray matter. Except for the increase in 18:2, acyl group changes in choline phosphoglycerides between tumors and controls were not as extensive as in the ethanolamine phosphoglycerides. The characteristic features of phospholipids and their constituent acyl groups of tumors were often present in all the subcellular fractions. Although the acyl group profiles of the tumor phosphoglycerides were in closer resemblance to the fetal brain than to the adult brain, other differences were observed. Results indicate that neoplastic brain cells are unique in their cellular composition, and consequently they deviate from the normal neurons and glials in metabolism and functions.


Phosphonolipids. XXVI. Mixed-acid phosphonocephalins: synthesis of alpha'-stearoyl-beta-oleoyl-l-alpha-glyceryl-(2-aminoethyl)phosphonate.


Phosphorylation of a zymogen granule membrane polypeptide from rat pancreas.


Phosphorylation of nuclear proteins during muscle differentiation in vitro.


Phosphorylation of proteins in rat liver. Endogenous phosphorylation and dephosphorylation of proteins from smooth and rough endoplasmic reticulum and free ribosomes.


Photoinactivation of ammonia oxidation in Nitrosomonas. Photoinactivation of ammonia oxidation in cells of Nitrosomonas was shown to follow first-order kinetics with a rate constant proportional to incident light intensity. The action spectrum for photoinactivation consisted of a broad peak in the ultraviolet range, where both hydroxylamine and ammonia oxidation were affected, and a shoulder at approximately 410 nm where only ammonia oxidation was affected. In photoinactivated cells, hydroxylamine but not ammonia was oxidized to nitrite and hydroxylamine but not ammonia caused reduction of cytochromes in vivo. The amount per cell of the following constituents was not measurably altered by photoinactivation: cytochromes b, c, a, and P460; ubiquinone; phospholipid; free amino acids; hydroxylamine-dependent nitrite synthetase; nitrite reductase; p-phenylenediamine oxidase; and cytochrome c oxidase. Malonaldehyde or lipid peroxides were not detected in photoinactivated cells. Photoinactivation was prevented (i) under anaerobic conditions, (ii) in the presence of methanol, allylthiourea, thiosemicarbazide, hydroxylamine, ethylxanthate, or CO at concentrations wich caused 100% inhibition of ammonia oxidation, and (iii) at concentrations of ammonia or hydroxylamine which gave a rapid rate of nitrite production. Recovery of ammonia oxidation activity in 90% inactivated cells took place in 6 h, required an energy and/or nitrogen source, and was inhibited by 400 mug of chloramphenicol per ml.


Phylogenetic aspects of adrenocortical activity during the process of adaptation.


Physical and chemical properties of opossum hemoglobin. A hemoglobin containing glutamine at position 58 (E7) in the alpha subunit.


Physico-chemical properties of chromatin of mouse sperm nuclei in heterokaryons with Chinese hamster cells.


Plant sterol metabolism. Enzymatic cleavage of the 9beta, 19beta-cyclopropane ring of cyclopropyl sterols in bramble tissue cultures.


Plants gathered as foodstuffs by the Transkeian peoples.


Plasma cortisol in children with fulminating meningococcal infection.


Plasma adenosine 3',5'-cyclic monophosphate in human hypertension. In a previous study we observed an increase in urinary cyclic AMP in labile hypertension in the upright position and during isoproterenol infusion, in contrast to a decrease in control subjects. In the present study we measured the plasma level of cyclic AMP in control subjects and patients with various types of hypertension. We obtained the following results: (1) plasma cyclic AMP increases in response to upright posture in control subjects and hypertensive patients; (2) values of cyclic AMP in the recumbent and upright positions are comparable in control subjects and patients with essential hypertension, but are significantly higher in those with true renovascular hypertension due to bilateral renal artery stenosis; (3) propranolol inhibits the increase of plasma cyclic AMP in response to posture in control subjects, but has an opposite effect in labile hypertension where there is a further increase; (4) the rise in blood pressure in pheochromocytoma is associated with a considerable increase in plasma cyclic AMP.Present and previous data suggest that kidney handling of cyclic AMP is abnormal in hypertension, and that the specific defect may be related to the type of hypertension.


Platelet electrophoresis: the present position.


Plasminogen, the serum proenzyme activated by factors from cells transformed by oncogenic viruses.


Plasma post-heparin lipolytic activity in patients with malabsorption: effect of intravenous fat administration.


Platelet retention in glass bead columns: further evidence for the importance of ADP.


Polymyositis without inflammation.

Polymyositis without inflammation.


Polypeptide composition of rat high density lipoprotein: characterization by SDS-gel electrophoresis.


Polypeptide distribution of the main lipoprotein density classes separated from human plasma by rate zonal ultracentrifugation.


Population dynamics of mitochondria. I. A model for the role to ACTH in the degradation of adrenocortical mitochondria.


Possible dapsone-induced peripheral neuropathy in dermatitis herpetiformis.


Postsynaptic responses of motor cortex neurons of cats to sensory stimulation of different modalities.


Preliminary crystallographic data for cytochrome c' of Rhodopseudomonas palustris.


Prenatal viral infection with particular reference to cytomegaloviruses.


Preparation and properties of a cell-free, hormonally responsive adenylate cyclase from guinea pig brain.


Preparation of avian ribosomes with low levels of contaminating elongation factors.


Preparation of adenosine nucleotide derivatives suitable for affinity chromatography. Methods of synthesizing a series of chemically-defined AMP, ADP, ATP, adenylyl imidodiphosphate and pyrophosphate derivatives suitable for affinity chromatography are extensively described. Each derivative has a single primary amino group at the end of a hexamethylene ;spacer' chain for attachment to CNBr-activated agarose. The synthesis of the derivative where the ;spacer' arm is attached directly to the 8 position of the adenine ring to produce 8-(6-aminohexyl)amino-AMP involves the direct bromination of AMP in the 8 position followed by displacement of the halogen by 1,6-diaminohexane. This monophosphate derivative can then be converted into the corresponding di- or triphosphate forms by direct phosphate condensation with carbonyl di-imidazole. A second series of adenosine phosphate derivatives with the phosphate moieties unsubstituted has been similarly prepared from N(6)-(6-aminohexyl)-AMP (Guilford et al., 1972). A third type of ligand has been synthesized by condensing the phosphoryl imidazolide of AMP with 6-aminohex-1-yl phosphate. This compound, P(1)-(6-aminohex-1-yl) P(2)-(5'-adenosyl) pyrophosphate, has an unsubstituted adenine ring. The synthesis of a fourth type of ligand, 6-aminohex-1-yl pyrophosphate, was done by heating 6-aminohexan-1-ol with crystalline pyrophosphoric acid under reduced pressure. The structures of the synthesized compounds were confirmed by chemical, electrophoretic and chromatographic methods and by u.v. spectrometry. The general applicability of the synthetic methods used is discussed in relation to the preparation of other affinity adsorbents. Examples are given where these derivatives have been successful in reversibly binding dehydrogenases, kinases and myosin and its proteolytic subfragments. The partial purification of rat liver glucokinase on an ADP derivative is shown.


Preparation of 3-chloroacetylpyridine adenine dinucleotide: an alkylating analogue of NAD+.


Pressure response of pulmonary vasculature to vasoconstricting agents.


Prevention by metallothionein of cadmium-induced inhibition of vitamin D activation reaction in kidney.


Prediction of breast cancer response to endocrine therapy.


Prevention of hemolysis by bivalent metal ions during virus-induced fusion of erythrocytes with Ehrlich ascites tumor cells.


Primary cause of decreased functional reserve in the liver of icteric patients and rats.


Primary mucinous (adenocystic) carcinoma of the skin.


Prevention of the generalized Shwartzman reaction and endotoxin lethality by polymyxin B localized in tissues. Pretreatment with multiple doses of polymyxin B and colistimethate was evaluated as to its ability to sequester sufficient antibiotic in tissues to neutralize the effects of endotoxin in three animal models. Animals were challenged with endotoxin 24, 48, or 72 h after the last dose of antibiotic when there was minimal or not detectable drug in serum. Pretreatment with polymyxin B was successful in preventing the generalized Shwartzman reaction in rabbits and reducing endotoxin lethality in mice; however, large doses (20 mg per kg per day for 2 or 4 days) were required. Prolongation by more than 24 h of the interval between the last dose of polymyxin B and endotoxin challenge resulted in reduction or loss of protection. Dogs were unable to tolerate the high polymyxin B dosage which was protective in the mouse and rabbit. Lower, nontoxic doses of polymyxin B in dogs did not prevent endotoxin shock and lethality, even when challenged as soon as 1 h after the last dose. Pretreatment with colistimethate was ineffective in all three animal models.


Primary hepatoma and invasive lobular carcinoma of breast without cirrhosis.


Primary structure of lobster-muscle arginine kinase. Isolation and characterization of the fragments produced by cyanogen-bromide cleavage.


Probenecide sensitive 3'-5'-cyclic AMP secretion by isoproterenol stimulated glial cells in culture.


Proceedings: Effect of temperature on the regulatory properties of poikilothermic enzymes.


Proceedings: Feed-back control of corticotrophin in the rat.


Proceedings: Effects of electrical and chemical stimulation of the central nervous system on sodium chloride and water intake.


Proceedings: Humoral control of periodical changes of nervous excitability.


Proceedings: Glycine receptors in the cerebral cortex are probably transport receptors.


Proceedings: Do the anterior pituitary and adrenal cortex participate in the reflex response of the adrenal medulla to arterial hypoxia?


Proceedings: Intraneuronal distributions and metabolic pathways of the adrenergic neurotransmitter.


Proceedings: Hydroxylation of steroids and electron microscopy in adrenals from rats bearing an ACTH secreting tumor.


Proceedings: Malacoplakia in childhood.

Proceedings: Malacoplakia in childhood.


Proceedings: Liver and adipose tissue metabolism in rats with acute insulin deficiency induced by total pancreatectomy.


Proceedings: Modification of the receptive field properties of neurones in the visual cortex by bicuculline, a GABA antagonist.


Proceedings: Neurotrophic effects upon muscles and receptors of the cat's tongue.


Proceedings: Pancreas and hypophysis in liver ketone body production.


Proceedings: Physiological implication of the metabolism of androgen in brain.


Proceedings: Primary hepatic cancer in childhood.


Proceedings: Some similarities and differences between renin and sustained pressor principle.


Proceedings: Regulatory mechanisms of sodium appetite.


Proceedings: Stimulation and inhibition of renin production in monolayer cultures of kidney cells.


Proceedings: The brain renin-angiotensin system. Its physiological role.


Proceedings: Synthetic angiotensin inhibiting peptides.


Proceedings: The effect of reserpine on pituitary-adrenocortical function in the rat.


Proceedings: The renin--angiotensin system in experimental renal hypertension.


Proceedings: The role of the presynaptic receptors in regulating noradrenaline overflow by nerve stimulation.


Prognostic value of ERG (oscillatory potential) in juvenile diabetics.


Proceedings: The mechanism of action of estrogens.


Prolactin stimulation of estrogen receptor in vitro in 7,12 dimethylbenz(A)anthracene-induced mammary tumor.


Prolonged culture of Visna virus in human astrocytes.


Prolylcarboxypeptidase in biological fluids.


Properties of feline leukemia virus. II. In vitro labeling of the polypeptides. Radioactive labeling of proteins from guanidine-hydrochloride disrupted feline leukemia virus was done in vitro by attaching [(3)H]methyl groups to protein amino groups with reductive alkylation. When in vitro labeled disrupted virus was analyzed by gel filtration in the presence of 6 M guanidine-hydrochloride the expected six major and one minor protein peaks were detected. When the same preparation was analyzed by immunodiffusion, the three antigens tested were found to have retained their antigenic activity.


Properties of partially purified liver microsomal cytochrome P-450: acceptance of two electrons during anaerobic titration.


Properties of the nicotinamide adenine dinucleotide-specific isocitrate dehydrogenase from Blastocladiella emersonii.


Properties of the abnormal human plasma lipoprotein (LP-X) characteristic of cholestasis after chemical modification with succinic anhydride.


Properties of the Sezary lymphoid cell. An ultrastructural analysis.


Prostaglandin inhibition of cartilage chondromucoprotein synthesis: concept of "intrinsic activity".


Properties of Clostridium perfringens (welchii) type-A alpha-toxin (phospholipase C) purified by electrofocusing.


Prostaglandin receptors in human and ovine adrenal glands: binding and stimulation of adenyl cyclase in subcellular preparations.


Protein kinases in testes of adult and prepuberal rats.


Protein metabolism in tumor cells at various stages of growth in vivo. Protein metabolism of Yoshida ascites hepatoma cells was studied in the early phase of logarithmic proliferation and in the following stage in which cell mass remains constant (resting phase). The rate of protein synthesis was measured by a short-time incorporation of [(8)H]lysine, while degradation was concurrently assessed by following the decrease of specific activity of [(14)C]lysine-labeled proteins. Most of the labeled amino acid injected intraperitoneally into the animal was immediately available for the tumor cells, with only a minor loss towards the extra-ascitic compartment. It was thus possible to calculate the dilution of the isotope in the ascitic pool of the lysine, which increased concurrently with the ascitic plasma volume. Amino acid transport capacity did not change in the log vs. the resting cells. This fact permitted the correction of the specific activity of the proteins synthesized by tumors in the two phases, taking into account the dilution effect. Protein synthesis was found to proceed at a constant rate throughout each of the two phases, although it was 30% lower during the resting as compared to the log phase. When cell mass attained the steady-state, protein degradation occurred at such a level as to balance the synthesis. Throughout the resting phase the amount of lysine taken up by the cells and renewed from the blood remained unchanged. Protein turnover, as studied in subcellular fractions, exhibited a similar rate in nuclei and microsomes, where it proceeded at a higher level than in mitochondria. On the whole, the results encourage the use of the Yoshida ascites hepatoma as a suitable model for studying protein turnover in relation to cell growth in vivo.


Proteolipids.

Proteolipids.


Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. The polypeptides from purified virions of a herpes simplex 1 (human herpes-virus 1) strain, F1, which had been passaged a limited number of times in cell culture after isolation, formed 33 bands on electrophoretic separation in polyacrylamide gels cross-linked with N, N'-diallyltartardiamide in contrast to a maximum resolution of only 24 to 25 bands in gels cross-linked with N, N'-methylenebisacrylamide. This increase in the number of bands was due chiefly to an improved separation of glycosylated polypeptides from nonglycosylated polypeptides with which they co-electrophoresed on methylenebisacrylamide cross-linked gels. Purified virions of HSV-1 [F1] had a protein/DNA mass ratio of 10.7 +/- 0.96, and based on a DNA molecular mass of 85 x 10(6) to 100 x 10(6) the estimated weight of virion polypeptides ranges from 16.4 to 19.4 x 10(-16) g. The number of molecules of each polypeptide per virion ranged from less than 50 to 1,500. Comparison of the virion polypeptides of two HSV-1 strains with similar isolation and limited passage history with those of four HSV-1 strains with histories of numerous passages outside the human host showed a number of nonrandom variations in virion polypeptides. Thus, although the virion polypeptides of two strains with similar isolation and limited passage history could not be differentiated, strains with extended passage histories differed markedly from each other and from the limited passage strains in the number and electrophoretic mobility of noncapsid polypeptides and notably in those of the envelope.


Protein-losing enteropathy in primary lymphoedema: mesenteric lymphography and gut resection.


Purification and characterization of Streptomyces sialidases. Some strains of Streptomyces produce sialidases. Two sialidases were purified over 1,000-fold from a culture filtrate of two Streptomyces species. They had the same properties in molecular weight, behavior to ions and other reagents, and substrate specificity. They showed very small differences in kinetic properties, pH optima, and heat stability. These Streptomyces sialidases differed markedly from Clostridium perfringens sialidase in molecular weight, p-chloromercuribenzoate sensitivity, and substrate specificity. Approximate molecular weights of the sialidases from Streptomyces and C. perfringens were 32,000 and 57,000, respectively. p-Chloromercuribenzoate (10(-3) M) caused complete inhibition of C. perfringens sialidase but not of Streptomyces sialidases.


Purification of the cAMP receptor protein by affinity chromatography.


Purification of messenger RNA and heterogeneous nuclear RNA containing poly(a) sequences.


Psychiatric drugs and trends. Review of the phenothiazines. II.


Psychiatric patients' impressions of teaching conferences.


Purification and regulatory properties of phosphoribulokinase from Hydrogenomonas eutropha H 16. 1. Phosphoribulokinase was purified 286-fold from extracts of autotrophically grown cells. 2. The enzyme had a molecular weight of 237000 and showed a pH optimum of 9.0 in both crude extracts and purified preparation. MgCl(2) was required for activity; full activation was obtained at 5mm-MgCl(2) and the K(m) was approx. 0.5mm. 3. The ATP-saturation curve was sigmoidal and the degree of positive co-operativity increased at higher MgCl(2) concentrations. The ATP-binding sites appeared to be non-interacting at low ribulose 5-phosphate concentrations. 4. Lineweaver-Burk plots for ribulose 5-phosphate showed abrupt transitions between apparently linear sections. The apparent K(m) and V(max.) values increased with increasing concentrations of ribulose phosphate. The transitions may be explained by a sequence of negative and positive co-operativity in the catalytic rate constants. 5. Phosphoribulokinase activity was inhibited by AMP and phosphoenolpyruvate and was activated by NADH. The presence of AMP or phosphoenolpyruvate increased s(0.5) (substrate concentration required for half-maximal velocity) for both ribulose 5-phosphate and ATP but V(max.) was not changed. The sigmoidicity of the ATP-saturation curve increased in the presence of AMP but was not affected by phosphoenolpyruvate. The transitions in the ribulose 5-phosphate-saturation curves were more abrupt in the presence of either inhibitor. NADH lowered the s(0.5) for both ribulose 5-phosphate and ATP. The activator did not affect the degree of positive co-operativity between ATP-binding sites, but the ribulose 5-phosphate-binding sites appeared to be non-interacting in its presence. 6. A sequence of positive and negative co-operativity in the interactions of AMP-binding sites was suggested by the Hill plots. In the presence of NADH (and phosphoenolpyruvate) the sensitivity to inhibition by AMP was less below a certain AMP concentration and increased above that concentration. 7. Examination of the interactions between ligands indicated that phosphoribulokinase can be regulated effectively by changes in effector concentrations similar to those reported to occur in vivo.


Purification of Escherichia coli ADPglucose pyrophosphorylase by affinity chromatography.


Pyrophosphate arthropathy: a clinical study of fifty cases.


Purification and propeties of (plus)-cis-naphthalene dihydrodiol dehydrogenase of Pseudomonas putida. Cells of Pseudomonas putida, after growth with naphthalene as sole source of carbon and energy, contain an enzyme that oxidizes (+)-cis-1(r),2(s)-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. The purified enzyme has a molecular weight of 102,000 and apparently consists of four 25,500 molecular weight subunits. The enzyme is specific for nicotinamide adenine dinucleotide as an electron acceptor and also oxidizes several other cis-dihydrodiols. However, no enzymatic activity was observed with trans-1,2-dihydronaphthalene, or the K-region cis-dihydrodiols of carcinogenic polycyclic hydrocarbons.


Purification of N-acetyl D-glucosamine-binding proteins by affinity chromatography.


P430, a possible primary electron acceptor in Rhodospirillum rubrum.


Quantitative aspects of iodothyronine binding by cytosol proteins of rat liver and kidney.


Psychiatric nursing: facilitating the development of a helping person.


Quantitative determination of bile acids and their conjugates using thin-layer chromatography and a purified 3alpha-hydroxysteroid dehydrogenase.


Rabbit intubation and halothane anesthesia.


Radiation treatment of intraocular cancer metastases.


Quantum chemical study of the thermodynamics, kinetics of formation and bonding of H2CN: relevance to prebiotic chemistry.


Radiation-killed BCG in the treatment of transplanted rat tumours.


Radiographic anatomy of normal cerebral deep medullary veins: criteria for distinguishing them from their abnormal counterparts.


Radiologic diagnosis of carcinoma of the biliary tree.


Rapid degradation of puromycyl peptides in hepatoma cells and reticulocytes.


Rapid determination of estriol of pregnancy by spectrodensitometry of thin layer chromatograms.


Radioimmunologic determination of plasma unconjugated estriol in normal and abnormal pregnancies with a specific antiserum to estriol.


Rapid isolation of a plant microsomal fraction by Mg2+--precipitation.


Rapidly fatal cytomegalovirus pneumonia in Hodgkin's disease.


Rapid diagnosis and typind of herpesvirus hominis by the fluorescent antibody method.


Rate constants for the reactions of OH radicals with the enzyme proteins as determined by the p-nitrosodimethylaniline method.


Re-examination of the sequence of the C-terminal tryptic fragment from porcine adrenocorticotropic hormone.


Reaction of nucleosides with N-trimethylsilylimidazole: separation of TMS derivatives of anomeric pyrimidine nucleosides by gas-liquid chromatography.


Reassociation and reactivation of lactic dehydrogenase from the unfolded subunits.


Recent advances in wound healing.

Recent advances in wound healing.


Reaction of alantolactone, an allergenic sesquiterpene lactone, with some amino acids. Resultant loss of immunologic reactivity.


Recent views on tremors and their treatment.


Recombination of human erythrocyte apoprotein and lipid. I. Interaction of apoprotein and lipid at the air-water interface.


Receptors and acceptors: a necessary distinction in hormone binding studies.


Recommended methods for the determination of four enzymes in blood.


Recent advances in glycogen metabolism.

Recent advances in glycogen metabolism.


Reduced nicotinamide adenine dinucleotide cytochrome o reductase associated with cytochrome o purified from Vitreoscilla. Evidence for an intermediate oxygenated form of cytochrome o.


Reduction of the pressor response to angiotensin and norepinephrine in the rat, induced by a renal peptide.


Regulation of amino acid transport in chick embryo heart cells. IV. Site and mechanisms of insulin action.


Regulation of adenosine 3':5'-monophosphate content in human astrocytoma cells by adenosine and the adenine nucleotides.


Regulation of cellular sterol flux and synthesis by human serum lipoproteins.


Recurrent herpes simplex virus infections.


Regulation of hormone stimulation of adipose tissue lipolysis by guanosine triphosphate.


Regulation of steroid biosynthesis.

Regulation of steroid biosynthesis.


Regulation of the mammalian pyruvate dehydrogenase multienzyme complex by Mg2+ and the adenine nucleotide pool.


Relative stabilities of the intermediate reducible crosslinks present in collagen fibres.


Regulation of synthesis of two immunologically distinct nucleic acid-dependent nucleoside triphosphate phosphohydrolases in vaccinia virus-infected HeLa cells. The two nucleic acid-dependent nucleoside triphosphate phosphohydrolases, previously purified from vaccinia virus cores, were shown to be immunologically distinct enzymes. Antiserum prepared against purified phosphohydrolase I and antiserum prepared against purified phosphohydrolase II only neutralized the activity of that enzyme used as antigen. Both enzymes were induced in HeLa cells after vaccinia infection. DNA-cellulose chromatography was used to purify the two phosphohydrolases from the cytoplasms of infected cells. The enzymes were identified by their different substrate specificities, nucleic acid dependence, and neutralization with specific antiserum. A third chromatographically separable nucleic acid-dependent phosphohydrolase similar to phosphohydrolase I in substrate specificity but not neutralizable by antiserum to either phosphohydrolase I or II, was also isolated from infected cells. No nucleic acid-dependent nucleoside triphosphate phosphohydrolase activity was detected by similar methods from uninfected HeLa cells. Formation of these virus-induced enzymes was prevented by actinomycin D and cycloheximide, indicating a requirement for de novo RNA and protein synthesis, respectively. The kinetics of induction and inhibition by cytosine arabinoside, an inhibitor of DNA synthesis, suggested that synthesis of the phosphohydrolases is a late viral function. Rifampin, an inhibitor of vaccinia virus growth which prevents virion assembly, had no inhibitory effect on the induction of the phosphohydrolases. This result was consistent with the finding that these enzymes exist in a soluble as well as in a particulate form in the cytoplasm of infected cells. Addition of another specific anti-poxviral drug, isatin-beta-thiosemicarbazone, to vaccinia-infected cells partially inhibited induction of the phosphohydrolases.


Relaxation of catabolite repression and loss of valine sensitivity in Escherichia coli K-12.


Release of lipopolysaccharide, phospholipids and enzymes from Salmonella enteritidis by ethylenediaminetetra-acetic acid.


Remarks about angiographic findings in cases of pontine tumor.


Renal transplantation in hypophosphatemia with vitamin D-resistant rickets.


Relaxation spectra of rabbit muscle lactate dehydrogenase and tetraiodofluorescein.


Renin-angiotensin-aldosterone system of a woman with Bartter's syndrome: Juxtaglomerular cell hyperplasia without hypertension.


Reovirus-associated mortality in broiler chickens.


Replication of mouse-tropic and xenotropic strains of murine leukemia virus in human x mouse hybrid cells. The replication of mouse-tropic and xenotropic strains of murine leukemia virus in human x mouse hybrid cells was investigated. NB-tropic strains of the leukemia virus replicated efficiently in several hybrid lines, including those that contained a complete complement of human chromosomes and many mouse chromosomes. In lines with only a few mouse chromosomes, NB-tropic viruses failed to replicate. N- and B-tropic viruses replicated in human x N-type and human x B-type cells, respectively. The N- and B-tropic viruses replicating in these hybrid cells retained their original tropism. The viral restrictive functions of the mouse Fv-1 locus were expressed in the hybrid cells, restricting the replication of N- and B-tropic strains in human x B-type and human x N-type mouse cells, respectively. In contrast to mouse-tropic viruses, AT-124 virus, a xenotropic strain, replicated in human but not in mouse cells or in hybrid cells containing a complete complement of human chromosomes and near complete complement of mouse chromosomes However, hybrid lines with only a few mouse chromosomes supported AT-124 replication. Thus, human genes in hybrid cells do not restrict the replication of mouse or xenotropic murine leukemia virus strains, while mouse genes in such cells restrict xenotropic leukemia virus replication and, as determined by the mouse Fv-1 phenotype, mouse-tropic murine leukemia virus. These results indicate that exogenously applied mouse-tropic and xenotropic oncornaviruses exhibit different patterns of restriction in human-mouse hybrid cells and that such hybrid cells may be used for genetic analysis of oncornavirus replication.


Replacement of ribothymidine by 5-methyl-2-thiouridine in sequence GT psi C in tRNA of an extreme thermophile.


Requirement for cell division for initiation of transcription of Rous sarcoma virus RNA. Stationary chicken embryo fibroblasts exposed to Rous sarcoma virus (RSV) did not synthesize virus RNA until after division of the infected cells. Initiation of RNA transcription after cell division resulted in transcription of the entire viral genome and was followed shortly thereafter by appearance of progeny virus. Addition of colchicine to RSV-infected stationary chicken embryo fibroblasts resulted in inhibition of cell division and a 24-h delay in the appearance of virus RNA. RSV-infected chicken embryo fibroblasts made stationary 10 to 15 cell generations after infection continued to produce an amount of progeny virus similar to that produced in dividing RSV-infected cells. Teh virus produced from cells made stationary after virus production had begun contained RNA synthesized in stationary cells. Therefore, division of the RSV-infected chicken embryo fibroblast is required for the initiation of transcription of virus RNA, but it is not required for the maintenance of transcription of virus RNA.


Requirement of L-serine for enzymic activation of carcinogen, 4-hydroxyaminoquinoline 1-oxide.


Respiratory deficiency in Saccharomyces carlsbergensis 4228 caused by thiamine and its prevention by pyridoxine.


Retinoblastoma in Norway.

Retinoblastoma in Norway.


Reticuloendothelial function in experimental injury and tolerance to shock.


Retrospective study of 117 patients with optic neuritis.


Ribonucleoprotein complexes containing nascent DNA-like RNA in the crude chromatin fraction of rat liver.


Rod outer segment phosphodiesterase: factors affecting the hydrolysis of cyclic-AMP and cyclic-GMP.


Role of cyclic nucleotides in cancer.

Role of cyclic nucleotides in cancer.


Role of cyclic AMP in reproductive processes.


Role of vasopressin and angiotensin in response of splanchnic resistance vessels to hemorrhage.


Rorschach responses subsequent to REM deprivation in schizophrenic and nonschizophrenic patients.


Sabin's vaccine used for nonspecific prevention of infant diarrhea of viral etiology.


Salmonellosis and shigellosis at Boston City Hospital. 2. Susceptibility of strains of Salmonellae and Shigellae to antibacterial agents.


Seasonal infection of Culex mosquitos and swine with Japanese encephalitis virus. A year-round study of the infection rates of JE virus in Culex mosquitos was made during 1970-71 in 2 hamlets in Taoyuan County, China (Province of Taiwan). JE virus was recovered from 5 of 314 pools of C. annulus, and from 1 of 22 pools of C. tritaeniorhynchus; these recoveries occurred during a 14-day period in July 1971. None of the 288 pools of C. p. fatigans, which had been collected between October and April, was positive. In addition, sentinel swine were assessed for antibody and virus. All the pigs became highly immune by 23 July; in each hamlet all the pigs had become infected within 1 week, virus being detected in them for only 1-2 weeks. Virus-positive mosquitos appeared to have obtained their infections at about the time that viraemia was occurring in the sentinel swine. These observations illustrate once again the lower infection rate and shorter duration of virus-positive mosquitos in China (Province of Taiwan) as compared with Japan.


Secretion of apolipoproteins in very low density and high density lipoproteins by perfused rat liver. The incorporation of labeled amino acids into the peptides of very low density lipoproteins (VLDL) and high density lipoproteins (HDL) secreted by perfused rat liver was studied using a Ringer-albumin solution in the perfusate in place of serum to diminish exchange of peptides between VLDL and HDL. Among the lipoproteins, the greatest release of protein, greatest incorporation of amino acid, and highest specific activity were found in VLDL. After separation of the delipidated peptides by electrophoresis on polyacrylamide gel, the incorporation into VLDL peptides was found to be 5-10 times as great as into HDL peptides. There was virtually no incorporation into the peptides of low density lipoproteins (LDL). Approximately 25% of the radioactivity incorporated into perfusate VLDL failed to enter the 13% polyacrylamide gel. The remaining radioactivity was distributed primarily among three peptide bands; one, found in the upper portion of the gel, contained 45% of the total, most of the remainder being found in two rapidly migrating bands. These three peptides appear to approximate those of human apo-C in relative electrophoretic mobility. Most of the HDL peptide radioactivity entering the running gel was found in a band that migrates slightly faster than the main VLDL band. A portion of the radioactivity of this major HDL band did not enter the running gel unless beta-mercaptoethanol was present. Greater separation of these two bands by polyacrylamide gel electrophoresis for 24 hr confirmed that the major bands in VLDL and in HDL were different. The rapidly moving peptides of HDL were found to contain very little radioactivity. Determination of the intensity of staining of carrier-free perfusate VLDL and HDL peptides produced a pattern similar to the incorporation of labeled amino acids. It is concluded that the rapidly moving peptides, which may contain activators of lipoprotein lipase, are only secreted as part of the VLDL.


Selection and application of factors for forecasting the epidemic time and severity of Japanese encephalitis prevalence.


Selective formation of desmosomes in chick cell reaggregates.


Semen matrix calculi in a rhesus monkey (Macaca mulatta).


Separate estimation of serum beta-lipoprotein cholesterol and pre-beta-lipoprotein cholesterol: clinical aspects.


Separation of urinary androstanediol and pregnanediol isomers by a combined gas-liquid chromatography-thin-layer chromatography method.


Separation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation. The major lipoprotein density classes (chylomicrons-VLDL, LDL, HDL(2) and HDL(3)) were isolated from human plasma in a two-step ultracentrifugal procedure using the Ti-14 zonal rotor. The isolation of the two major high density lipoprotein subclasses (HDL(2) and HDL(3)) was achieved in a 24-hr run using a nonlinear NaBr gradient in the density range of 1.00-1.40. The lipoproteins with a density < 1.063 found in the rotor's center were isolated in a second run of 140 min duration using a continuous linear NaBr gradient in the density range of 1.00-1.30. The isolated lipoproteins were analyzed for chemical composition and for electrophoretic mobility; purity of isolated fractions was checked by immunochemistry. The lipoproteins exhibited flotation rates, chemical compositions, and molecular weights similar to those found with the common sequential procedures in angle-head rotors. The amount of lipoprotein lipids in the bottom fraction of the zonal rotor was comparable to that of the angle-head rotor. The described method yields the main lipoprotein density classes free from albumin in a very short running time; compared with the rate-zonal techniques already in use, this method allows the quantitative separation of an additional lipoprotein density class (HDL(2)) without increasing the running time. Furthermore, this procedure proved to be suitable for isolation of plasma lipoproteins from subjects with various types and varying degrees of hyperlipoproteinemia.


Sequence dependent deamidation rates for model peptides of cytochrome C.


Serologic testing of various primate species maintained in a single outdoor breeding colony.


Serum hormone concentrations in ruminants during mammary growth, lactogenesis, and lactation: a review.


Serum parathyroid hormone concentrations in hypophosphataemic vitamin D resistant rickets.


Significance of respiratory virus isolations. A study in primary schoolchildren.


Simultaneous bilateral fluorescein angiography of the anterior eye.


Simple methods of preparing nicotinamide mononucleotide.


Simian herpesviruses and neoplasia.

Simian herpesviruses and neoplasia.


Single dose irradiation in treatment of cerebral metastases from bronchial carcinoma.


Sleep disorders. Recent findings in the diagnosis and treatment of disturbed sleep.


Sleeping pills and dream content.

Sleeping pills and dream content.


Sleep-wakefulness cycle in adult rats suffered from icterus gravis neonatorum.


Smallpox.

Smallpox.


Sleep and some psychoactive drugs.

Sleep and some psychoactive drugs.


So-called"virus-like" intranuclear filaments in brain autopsy material: a reappraisal on hand of two fatal cases of tick-borne encephalitis.


Soluble gonadotropin receptors of the rat ovary.


Sleep pattern and intelligence in functional mental retardation.


Sodium-potassium-activated adenosine triphosphatase (NaK-ATPase) activity in the rat lens. A histochemical and biochemical study.


Some aspects on nervous control of aqueous humour dynamics in the rabbit eye.


Soluble lymphocytic mediators. I. Inhibition of macrophage migration inhibitory factor production by drugs.


Some characteristics of corticosterone uptake by the dorsal hippocampus and the adenohypophysis in the rat.


Some chemical parameters of serum, cerebrospinal fluid, perilymph, and aqueous humor of the chinchilla.


Some histochemical reactions in the neurocytes of the trigeminal ganglion in rabbits.


Some properties of endothelial cells isolated from human umbilical cord vein.


Some parasitic and other natural diseases of the African elephant in the Kruger National Park.


Some reactions of 1,6-di-O-p-tolylsulphonyl-D-mannitol and its derivatives.


Some properties of the nitrogenase proteins from Clostridium pasteurianum. Molecular weight, subunit structure, isoelectric point and EPR spectra.


Some studies of adrenal suppression by alternate-day prednisone therapy.


Spacer arms in affinity chromatography: use of hydrophilic arms to control or eliminate nonbiospecific adsorption effects.


Specific inactivation of fructose 1,6-bisphosphatase from Saccharomyces cerevisiae by a yeast protease.


Specific RNA fragments in defective particles of bacteriophage MS2.


Specific interaction of 3H-FSH with rat testis binding sites.


Specificity of the retrograde axonal transport of nerve growth factor.


Spectral characteristics and interconversions of the reduced oxidized, and oxygenated forms of purified cytochrome o.


Specificity of the natural rat liver ribonuclease inhibitor towards nuclease activities of subcellular fractions.


Spin labelling of D-glyceraldehyde-3-phosphate dehydrogenase with specific reagents.


Spin-labeled polyuridylic acid. Formation of an intermediate structure.


Spin-labelled phosphorylase as a probe in a rabbit muscle glycogen particle fraction.


Speaking out on: social change, social policy and the helping professional.


Splenomegaly, sex, and other characteristics of laboratory animals used for primary isolations of Coxiella burnetii.


Spontaneous hematoma of the optic chiasma. Report of a case.


Spontaneous and stimulated secretion of QUSO-extractable immunoassayable ACTH in man.


Specific inhibition of the renin-angiotensin system: A key to understanding blood pressure regulation.


Spontaneous tumours of the central nervous system of laboratory rats.


Steroid metabolism in arrhenoblastoma.

Steroid metabolism in arrhenoblastoma.


Steroid-induced bilateral femoral head ischaemic necrosis.


Stimulation of a dopamine-sensitive adenylate cyclase in homogenates of rat striatum by a metabolite of piribedil (ET 495).


Stimulation by D-glucose of cyclic adenosine 3':5'-monophosphate accumulation and insulin release in isolated pancreatic islets of the rat.


Stimulation of melanogenesis in cultured melanoma cells by calciferols.


Stimulation of sodium transport in turkey erythrocytes by cyclic 3',5'-AMP.


Streptozotocin diabetes. Correlation with extent of depression of pancreatic islet nicotinamide adenine dinucleotide. The diabetogenic activity of streptozotocin has been correlated with a reduction in pyridine nucleotide synthesis in the mouse pancreatic islet. To determine the specificity of this reduction for diabetogenicity, a comparative study of streptozotocin, its cytotoxic moiety, 1-methyl-1-nitrosourea, and alloxan was performed. Streptozotocin administered intraperitoneally (i.p.) producd a dose-related reduction in islet NAD which was proportional to the degree of diabetogenicity. A diabetogenic dose, 200 mg/kg, attained a peak plasma N-nitroso intact streptozotocin concentration of 0.224 mumol/ml and reduced the mean islet NAD from a control of 0.78 to 0.15 pmol. At borderline, 150 mg/kg, and nondiabetogenic, 100 mg/kg, doses, plasma concentrations reached 0.161 and 0.136 mumol/ml, and NAD was 0.36 and 0.86 pmol/islet, respectively. 1-Methyl-1-nitrosourea, 100 mg/kg, attained a maximum N-nitroso intact 1-methyl-1-nitrosourea concentration of 0.162 mumol/ml and reduced the mean NAD to 0.58 pmol/islet, and was nondiabetogenic; 200 mg/kg attained a peak plasma concentration of 0.344 mumol/ml and depressed NAD to 0.38 pmol/islet, and was inconsistently diabetogenic. Islet NAD of 0.4 pmol/islet or greater is required for integrity of the beta cell. A diabetogenic dose of alloxan, 500 mg/kg, did not depress NAD, 0.85 pmol/islet, therefore confirming that its mechanism of diabetogenicity differs from that of streptozotocin. In vivo uptake of [methyl-(14)C]streptozotocin by islets was 3.8 times that of [methyl-(14)C]-1-methyl-1-nitrosourea, whereas uptake by the exocrine pancreas favored 1-methyl-1-nitrosourea over streptozotocin 2.4:1. The decreased islet uptake of 1-methyl-1-nitrosourea correlates with the 3.5 times increased molar dosage required to produce islet NAD depression comparable to that of streptozotocin, 150 mg/kg. These studies indicate that the glucose carrier of streptozotocin facilitates uptake of its cytotoxic group, 1-methyl-1-nitrosourea, into islets.


Structural and functional changes in human and experimental neuropathy.


Structural studies of a mitochondria-free plasma membrane fraction from rat liver.


Structure of bis(methylguanidinium) monohydrogen orthophosphate. A model for the arginine-phosphate interactions at the active site of staphylococcal nuclease and other phosphohydrolytic enzymes.


Structural membrane proteins and loosely associated proteins of the sarcoplasmic reticulum. The protein composition of sarcoplasmic-reticulum vesicles, either unpurified or after fractionation on sucrose gradients, and with or without previous osmotic shock and sonication, was investigated by electrophoresis in acid polyacrylamide gels. The pattern of release of loosely bound proteins is discussed with respect to their localization in the interior of the vesicles.


Some fundamental considerations in the study of sleep.


Structure-function relationship in rabbit muscle glyceraldehyde-3-phosphate dehydrogenase. Trinitrophenylation of the lysine residues.


Structure-function relationships in foot-and-mouth disease virus.


Studies of insulin, growth hormone and prolactin binding: tissue distribution, species variation and characterization.


Studies of insulin, growth hormone and prolactin binding: ontogenesis, effects of sex and pregnancy.


Studies on androgen and estrogen uptake by rat hypothalamus.


Studies on free and 5 S RNA-bound ribosomal GTPase and ATPase.


Studies of the polysaccharide fraction from the lipopolysaccharide of Pseudomonas alcaligenes. Studies of the lipopolysaccharide of Pseudomonas alcaligenes strain BR 1/2 were extended to the polysaccharide moiety. The crude polysaccharide, obtained by mild acid hydrolysis of the lipopolysaccharide, was fractionated by gel filtration. The major fraction was the phosphorylated polysaccharide, for which the approximate proportions of residues were; glucose (2), rhamnose (0.7), heptose (2-3), galactosamine (1), alanine (1), 3-deoxy-2-octulonic acid (1), phosphorus (5-6). The heptose was l-glycero-d-manno-heptose. The minor fractions from gel filtration contained free 3-deoxy-2-octulonic acid, P(i) and PP(i). The purified polysaccharide was studied by periodate oxidation, methylation analysis, partial hydrolysis, and dephosphorylation. All the rhamnose and part of the glucose and heptose occur as non-reducing terminal residues. Other glucose residues are 3-substituted, and most heptose residues are esterified with condensed phosphate residues, possibly in the C-4 position. Free heptose and a heptosylglucose were isolated from a partial hydrolysate of the polysaccharide. The location of galactosamine in the polysaccharide was not established, but either the C-3 or C-4 position appears to be substituted and a linkage to alanine was indicated. In its composition, the polysaccharide from Ps. alcaligenes resembles core polysaccharides from other pseudomonads: no possible side-chain polysaccharide was detected.


Studies on pyrimidine biosynthesis in the granulocytes of patients receiving gold therapy for rheumatoid arthritis.


Studies on polynucleotides. The linkage of deoxyribopolynucleotide templates to cellulose and its use in their replication.


Studies on proteins of animal ribosomes. XVII. Quantitative aspects of enzymatic phosphorylation of ribosomal proteins and particles from rat liver.


Studies on the biology of the lung mite, Pneumonyssus simicola banks (Acarina: Halarachnidae) and diagnosis of infestation in macaques.


Studies on the control of development. Differences in the pattern of DNA binding proteins isolated from vegetative and sporulating cells of Bacillus subtilis.


Studies on the estrogen receptor in breast cancer.


Studies on the distribution of urea cycle enzymes in different regions of rat brain.


Studies on the immune response and pathogenesis of Sendai virus infection of mice. II. The immunoglobulin class of plasma cells in the bronchial sub-mucosa.


Studies on the interaction of concanavalin A with major density classes of human plasma lipoproteins. Evidence for the specific binding of lipoprotein B in its associated and free forms.


Studies on the minor components of the organic matrix of human dentine.


Studies on the phosphorylation of nucleolar proteins. Identification of a nucleolus-associated protein kinase activity.


Studies on the ultrastructure of the blood-aqueous barrier in the rabbit.


Studies on 14C-imipramine metabolism in the isolated perfused rat liver.


Studies with foot-and-mouth disease virus in British deer (red, fallow and roe).


Studies with foot-and-mouth disease virus in British deer (red, fallow and roe). II. Recovery of virus and serological response.


Study of the reactions of blood with artificial surfaces. Use of the thrombogenerator.


Subacute sclerosing panencephalitis (SSPE). Reappraisal of nuclear, cytoplasmic and axonal inclusions ultrastructural study of eight cases.


Subcellular distribution of sphingomyelinase and other acid hydrolases in different parts of the calf lens.


Substructure of amphibian motor end plate. Evidence for a granular component projecting from the outer surface of the receptive membrane. End-plate membrane has been examined at amphibian myoneural junctions by means of transmission electron microscopy of thin tissue sections. The postjunctional membrane exhibits morphologically specialized dense, convex patches which are located superficially facing the axon terminal but do not extend into the depths of the junctional folds. In the specialized regions the plasma membrane is approximately 120 A thick and trilaminar. The outer dense lamina is thickened by the presence in it of granular elements approximately 60-120 A in diameter which are spaced semiregularly at approximately 100-150-A intervals and which border the junctional cleft directly. In these regions the concentration of the granules is of the order of approximately 10(4)/microm(2), which is in the same range as the estimated concentration of receptor sites at other vertebrate cholinergic junctions. Filamentous projections can sometimes be seen extending from the granules to the overlying basement membrane, and in oblique views a reticular pattern may appear both in these patches and in the basement membrane. The cytoplasmic surface of the specialized membrane is covered with an amorphous and filamentous dense material whose distribution coincides with that of the granules visible in the outer layer and which may be connected to them across the membrane. In unosmicated specimens stained with permanganate and uranyl acetate the specialized regions exhibit the same morphological features but stand out sharply in contrast to adjacent areas of unspecialized membrane which appear only faintly. Such preparations are particularly useful in assessing the extent of the specialized membrane. It is proposed that the granules visible at the outer surface of the end-plate membrane represent acetylcholine receptors and that in amphibians, as in annelids, the receptors at myoneural junctions are concentrated into patches which occupy less than the total postjunctional membrane surface area.


Subunit structure of testicular hyaluronidase.


Sugar catabolism in Aquaspirillum gracile. Aquaspirillum (Spirillum) gracile is one of the few spirilla that cause acidification of the medium when cultured with sugars. Acidic reactions have been reported only for d-glucose, d-galactose, and l-arabinose, and the mode of attack of these sugars has not been previously investigated. The soluble portion of extracts of glucose-cultured cells of A. gracile ATCC 19624 was found by spectrophotometric methods to contain enzyme activities characteristic of the Entner-Doudoroff and Embden-Meyerhof-Parnas pathways. No activity for 6-phosphogluconate dehydrogenase (EC 1.1.1.44) was detected. Pyridine nucleotide-linked dehydrogenase activities for l-arabinose and d-galactose (EC 1.1.1.46 and EC 1.1.1.48) occurred in the soluble fraction of cells cultured with either sugar. Glucose-cultured cells contained not only glucokinase (EC 2.7.1.2) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) activities but also glucose dehydrogenase (EC 1.1.1.47) activity. Enzymes capable of oxidizing gluconate were not detectable, but gluconokinase (EC 2.7.1.12) activity was present. Paper chromatographic analysis of the spent culture supernatant media from glucose-cultured cells indicated an accumulation of gluconic acid, and this was confirmed by enzymatic methods. Evidence is presented for the production of d-galactonic and l-arabonic acids in cultures containing d-galactose or l-arabinose, respectively.


Superoxide dismutase activity in WI-38 cell cultures: effects of age, trypsinization and SV-40 transformation.


Surface glycosyltransferases on cultured mouse fibroblasts.


Survival of Entamoeba and related Amoebae at low temperature. I. Viability of Entamoeba cysts at 4 degrees C.


Synaptic regulation of specific protein synthesis in an identified neuron.


Synergism between polymyxins, polysorbate and antimetabolites of folic acid synthesis, and a paper disc technique for routine testing for synergism.


Synthesis and degradation of the major hepatic phosphofructokinase isozyme in rat liver and hepatoma 3924-A.


Synthesis of (alpha-32P) ribo- and deoxyribonucleoside 5'-triphosphates.


Synthesis of galactosylphytosphingosine and galactosylceramides containing phytosphingosine.


Synthesis of substituted piperidino carbamides: correlation between CNS effects and selective inhibition of NAD-dependent oxidations.


Systemic chromomycosis in Rana pipiens.

Systemic chromomycosis in Rana pipiens.


Synthesis of viral-specific RNA in cells infected with the parvovirus, Kilham rat virus. We have studied the viral-specific RNA synthesized after infection of a permissive cell line with Kilham rat virus (KRV). The RNA was shown to be virus specific by analysis of its nucleotide base ratios and by hybridization with KRV and cellular DNA. Viral RNA is synthesized as early as 2 h after infection. This viral RNA synthesis occurs before viral progeny DNA synthesis which is initiated at 7 to 8 h after infection. The predominant viral RNA synthesized before and after viral progeny DNA synthesis has a sedimentation coefficient of approximately 18S in dimethylsulfoxide-sucrose gradients and a calculated molecular weight of 6.5 x 10(5) to 7.5 x 10(5). KRV contains a molecule of single-stranded DNA with a molecular weight of approximately 1.6 x 10(6). If the viral-specific 18S RNA is a homogenous species, it would account for 40 to 50% of the viral genome. A small amount of 26S viral RNA with a molecular weight of 1.6 x 10(6) to 1.7 x 10(6) can also be detected. If this 26S RNA is a single viral-specific entity, it could represent a transcription of the entire KRV genome.


Testosterone metabolism and 5 alpha-dihydrotestosterone-binding macromolecule in rat kidney.


The adenohypophysial response to stress and its hypothalamic control.


Target cell polarity and membrane phosphorylation in relation to the mechanism of action of antidiuretic hormone. The plasma membrane of the bovine renal collecting duct epithelial cell has been resolved into its apical (luminal) and basal-lateral (contraluminal) components by free flow electrophoresis. The contraluminal, but not the luminal, membrane was found to contain antidiuretic hormone-sensitive adenylate cyclase. The luminal membrane was found to contain a cyclic 3':5'-adenosine monophosphate-sensitive self-phosphorylating system consisting of a membrane-bound protein kinase and its membrane-bound substrate(s); this intrinsic protein kinase was not present in the contraluminal membrane. These findings provide direct evidence that the initiating steps in the action of antidiuretic hormone on the kidney take place at the contraluminal pole of the hormonesensitive target cell and that the late or terminal steps occur at the luminal pole, where they involve an alteration in the level of membrane phosphorylation.


The advantages of drosophila for mutation studies.


The amino acid sequences of the phosphorylated sites in troponin-I from rabbit skeletal muscle.


The binding of dexamethasone and triamcinolone acetonide to glucocorticoid receptors in rat skeletal muscle.


The binding of quinacrine mustard to nucleic acids.


The calcium ionophore A 23 187 as a probe for studying the role of Ca2+ ions in the mediation of carbachol effects on rat salivary glands: protein secretion and metabolism of phospholipids and glycogen.


The cardiodynamic effects and mechanism of action of bretylium tosylate.


The cellular basis of immunosuppression caused by the radiation leukaemia virus.


The carcinogenic effects of dimethylnitrosamine and nitrosomethylurea in European hamsters (Cricetus cricetus L.). The carcinogenic effects of dimethylnitrosamine (DMN) and nitrosomethylurea (NMU) injected subcutaneously at 3 different dose levels in European hamsters were studied. DMN induced malignant haemangioendotheliomata of the liver and kidney, hepatocellular carcinomata and, in one animal, a cholangiocellular carcinoma. The effect of NMU was localized at the site of administration and resulted in subcutaneous fibrosarcomata, carcinosarcomata or epidermal carcinomata.


The association of congenital malformations and malignant tumors in infants and children.


The chemical basis for the action of the vancomycin group of antibiotics.


The continuing evolution of an estrogen-receptor model.


The control of glycogen synthesis in the liver.


The control of myelin synthesis and inborn errors of metabolism.


The covalently bound flavin prosthetic group of beta-cyclopiazonate oxidocyclase.


The biological oxidation of aromatic heterocyclic amines.


The demonstration of protein synthesis-dependent repair replication in Escherichia coli after treatment with the mono-alkylating agent ethyl methane sulphonate.


The development of a new selective medium for the isolation of Brucella abortus from contaminated sources.


The diagnostic value of radioimmunoassay in clinical medicine. Discussion.


The diagnosis and management of myasthenia gravis.


The dyslipoproteinemias.

The dyslipoproteinemias.


The distribution of genetic polymorphisms in patients with Down's syndrome.


The effect of caffeine on the survival of u.v.-irradiated herpes simplex type 1 virus.


The effect of angiotensin-II intravenous infusion on plasma renin activity and prostaglandins A, E, and F levels in the uterine vein of the pregnant monkey.


The effect of corticotropin on mouse spleen colony-forming cells.


The effect of cAMP on phospholipid metabolism.


The effect of disinfectants on bacteriophage phi chi X 174.


The effect of early undernutrition on the calibre spectrum of the rat optic nerve. Amorphometric ultrastructural study.


The effect of edema, hydrocortisone acetate, concurrent viral infection and immunization on the clearance of Pasteurella hemolytica from the bovine lung. The influence of pulmonary edema, hydrocortisone, immunization against Pasteurella hemolytica and concurrent infection with parainfluenza-3 virus upon pulmonary clearance of aerosolized P. hemolytica was studied in 31 calves. Following the various treatments calves were challenged with an aerosol of P. hemolytica. One control calf was killed immediately after the aerosol and the numbers of bacteria in the lung taken as 100%. Two calves were killed four hours after challenge and the numbers of bacteria in the lungs were compared to the 100% of the control calf. The result was the percentage clearance of bacteria at four hours. PULMONARY EDEMA WAS INDUCED BY THREE DIFFERENT METHODS: by an aerosol of histamine, by intravenous injection of endotoxin and by intravenous injection of croton oil emulsion. The edema impaired the clearance of P. hemolytica, which was reflected in high numbers of P. hemolytica present in the lungs at four hours after challenge: 260% after histamine, 300% and 400% after endotoxin and 92% after croton oil. SIX DAYS OF TREATMENT OF FOUR CALVES WITH HIGH DOSES OF HYDROCORTISONE ACETATE PRODUCED INCONSISTENT RESULTS: two calves treated with a higher daily dose (36 mg/kg) had normal clearance whereas two calves treated with a lower dose had pulmonary edema and displayed lowered clearance with 111% and 31% respectively of P. hemolytica retained in the lungs four hours after challenge. Immunization of calves by three different methods, a subcutaneously injected bacterin of P. hemolytica (2 calves), single aerosol (2 calves) and four aerosols (4 calves) of live P. hemolytica was reflected in an accelerated pulmonary clearance of P. hemolytica (with a mean of 1.55% of bacteria retained at four hours). Concurrent infection with parainfluenza-3 virus did not lower the clearance of P. hemolytica in the lungs of 12 calves over 15 days except on the first day following the exposure to parainfluenza-3 virus. These calves had hemagglutinating antibodies against P. hemolytica before exposure.


The effect of glucagon on the liver cell membrane potential. 1. Intracellular recordings of membrane potential were made from superficial cells of isolated mouse liver segments superfused with physiological salt solutions.2. The mean resting cell membrane potential was -39.4 mV.3. Glucagon caused a dose-dependent membrane hyperpolarization which was detectable at 10(-9)M and maximal (7 mV) at 10(-7)M. The hyperpolarization started within half a minute after exposure to glucagon. Secretion (2 x 10(-7)M) had no effect on the membrane potential.4. Adrenaline (10(-6)M) and isoprenaline (10(-6)M) also caused membrane hyperpolarization (4-6 mV). The effect of isoprenaline, but not that of adrenaline, was blocked by propranolol (5 x 10(-6)M).5. Dibutyryl adenosine 3',5'-monophosphate (10(-3)M) caused a membrane hyperpolarization of 4-8 mV.6. In the absence of extracellular K or the presence of Strophanthin-G (10(-3)M) the resting potential was decreased and the response to glucagon reduced. During exposure to a solution containing 20 mM-K the resting potential was slightly enhanced and the amplitude of the glucagon-induced hyperpolarization reduced compared with control conditions.7. It is concluded that the effect of glucagon on the membrane potential is due to an interaction with specific membrane receptors probably leading to activation of the membrane-bound adenyl cyclase. It is probable that the hyperpolarization is mediated by cyclic AMP. The hyperpolarization induced by glucagon is dependent on a normal function of the membrane Na-K pump.


The effect of growth conditions on NAD+ and NADH concentrations and the NAD+:NADH ratio in normal and transformed fibroblasts.


The effect of hepatic injury upon the development of amoebic liver abscess in hamsters.


The effect of hydrocortisone and bromodeoxyuridine (BUdR) on the Epstein-Barr herpes virus in human lymphoblastoid cell lines.


The effect of intravenously administered prostaglandin E2 on rat uterine 3',5'-adenosine monophosphate.


The effect of noradrenaline and tyramine on cardiac contractility, cyclic AMP, and phosphorylase a in normal and hyperthyroid rats.


The effect of polychlorinated biphenyls on liver gluconeogenic enzyme activities in rats during exposure to cold.


The effect of tyrosinase on glyceraldehyde-3-phosphate dehydrogenase and glycerophosphate dehydrogenase.


The effects of epinephrine, prostaglandins, and their antagonists on adenosine cyclic 3',5'-monophosphate concentrations and motility of the rat uterus.


The effects of eye movement and dark-adaptation on transmission through the visual pathways of unrestrained cats.


The effects of hyperosmolarity on the blood-brain barrier. A morphological and physiological correlation.


The effects of parental composition on recidivism rates in delinquent girls.


The effects of infusions of synthetic adrenocorticotrophin in the conscious calf. 1. A technique is described by which the whole of the effluent blood from the right adrenal gland can be collected as required from conscious, unrestrained calves. The technique may be used to measure adrenal blood flow gravimetrically and to compute the output of adrenal hormones under various conditions in the normal calf.2. In a group of seven calves mean cortisol output from the right adrenal gland was found to vary between 20 and 40 ng.kg(-1) min(-1) and corticosterone between 6 and 18 ng.kg(-1) min(-1) during a 2 hr period, 24 hr after surgery.3. Intravenous infusions of synthetic adrenocorticotrophin (5 ng.kg(-1) min(-1)) produced a significant increase in the output of both cortisol and corticosterone within 5 min. The output of both hormones rose to maximal values within 10-20 min and mean values of approximately 300 ng.kg(-1) min(-1) (cortisol) and 120 ng.kg(-1) min(-1) (corticosterone) were maintained thereafter for the duration of the infusion (120 min). The output of both steroids fell to values comparable with those observed initially within 45-60 min after the infusion was discontinued.4. These changes in glucocorticoid output in response to adrenocorticotrophin produced a significant rise in the concentration of both cortisol and corticosterone in peripheral plasma. It is noteworthy that the rise in the mean corticosterone concentration in the peripheral plasma was substantially less than that which might be expected from relating the rise in mean plasma cortisol concentration to cortisol output.5. The results of control experiments have eliminated the possibility that the sampling procedure might itself increase steroid output or peripheral plasma concentration. Comparison of results from calves of widely disparate ages (8-38 days) provided no evidence that either the resting output of cortisol or corticosterone or the response to adrenocorticotrophin changes with age within the range examined.6. Infusion of adrenocorticotrophin (5 ng.kg(-1) min(-1)) also stimulated an abrupt rise in adrenal blood flow; mean resting flow (210 +/- 23 mul.kg(-1)) increased by approximately 30% within 5 min and attained peak values (355-365 mul.kg(-1) min(-1)) between 10 and 30 min. Thereafter, adrenal blood flow steadily decreased and then fell rapidly to within the resting range when the infusion was terminated. No significant changes in heart rate or aortic blood pressure occurred during these infusions.7. The results are discussed in relation to those obtained in other species and under differing conditions by other workers.


The effect of REM deprivation on depression.


The effects of thallium salts, with particular reference to the nervous system changes. A report of three cases.


The epithelium of the urinary bladder of rock hyrax.


The effects of theophylline on cyclic adenosine monophosphate metabolism in lymphocytes from open-angle glaucoma patients.


The human plasma kallikrein-kinin system.


The identification of intermediates in the reaction of pig heart lactate dehydrogenase with its substrates. Pig heart lactate dehydrogenase was studied in the direction of pyruvate and NADH formation by recording rapid changes in extinction, proton concentration, nucleotide fluorescence and protein fluorescence. Experiments measuring extinction changes show that there is a very rapid formation of NADH within the first millisecond and that the amplitude of this phase (phase 1) increases threefold over the pH range 6-8. A second transient rate (phase 2) can also be distinguished (whose rate is pH-dependent), followed by a steady-state rate (phase 3) of NADH production. The sum of the amplitudes of the first two phases corresponds to 1mol of NADH produced/mol of active sites of lactate dehydrogenase. Experiments that measured the liberation of protons by using Phenol Red as an indicator show that no proton release occurs during the initial very rapid formation of NADH (phase 1), but protons are released during subsequent phases of NADH production. Fluorescence experiments help to characterize these phases, and show that the very rapid phase 1 corresponds to the establishment of an equilibrium between E(NAD) (Lactate) right harpoon over left harpoon H(+)E(NADH) (Pyruvate). This equilibrium can be altered by changing lactate concentration or pH, and the H(+)E(NADH) (Pyruvate) species formed has very low nucleotide fluorescence and quenched protein fluorescence. Phase 2 corresponds to the dissociation of pyruvate and a proton from the complex with a rate constant of 1150s(-1). The observed rate constant is slower than this and is proportional to the position of the preceding equilibrium. The E(NADH) formed has high nucleotide fluorescence and quenched protein fluorescence. The reaction, which is rate-limiting during steady-state turnover, must then follow this step and be involved with dissociation of NADH from the enzyme or some conformational change immediately preceding dissociation. Several inhibitory complexes have also been studied including E(NAD+) (Oxamate) and E(NADH) (Oxamate') and the abortive ternary complex E(NADH) (Lactate). The rate of NADH dissociation from the enzyme was measured and found to be the same whether measured by ligand displacement or by relaxation experiments. These results are discussed in relation to the overall mechanism of lactate dehydrogenase turnover and the independence of the four binding sites in the active tetramer.


The identification of phosphatidylglycerol in the rat, rabbit, monkey and human lung.


The immunological system and the development of central nervous system lesions in acute viral infections.


The immunological basis of glomerulonephritis.


The incidence of cytomegalovirus among mentally retarded and microcephalic children in a state institution.


The information content of protein amino acid sequences.


The in vivo effects of leucine dehydrogenase from Bacillus sphaericus.


The inhibition of brain choline kinase by hemicholinium-3.


The inhibition of ribonuclease activity and the isolation of polysomes from leaves of the french bean, Phaseolus vulgaris.


The intake, digestion and utilization of food and growth rate of lambs affected by Trichostrongylus colubriformis.


The interaction of apolipoprotein-serine with phosphatidylcholine.


The interaction of uterine estrogen receptors with DNA.


The interaction of vasoactive intestinal polypeptide and secretin with liver-cell membranes.


The lipids of the alga Spirulina.

The lipids of the alga Spirulina.


The mechanism of reaction of Cys- 149 of D-glyceraldehyde-3-phosphate dehydrogenase with p-hydroxy-mercuribenzoate.


The metabolic response to exercise and exhaustion in normal and growth-hormone-deficient children.


The motor neuron surface and spinal shock.


The lysosomal enzymes in lymphocytes. II. Activated lymphocytes.


The motor unit profile of the rat soleus in experimental myopathy and reinnervation.


The nature and distribution of enzymes catalyzing the conjugation of glutathione with foreign compounds.


The normal short child. Community study of children in Newcastle upon Tyne.


The nucleotide sequence of the cytoplasmic initiator transfer RNA of a mouse myeloma cell.


The occurrence of intermediates in mitochondrial fatty acid oxidation.


The organization of extraocular muscles in Anura.


The pancreatic beta-cell recognition of insulin secretogogues. Influence of neuraminidase treatment on the release of insulin and the islet content of insulin, sialic acid, and cyclic adenosine 3':5'-monophosphate.


The poly(adenylic acid) sequence of mouse globin messenger RNA.


The peripheral lung pulse in pulmonary carcinoma. A cinedensigraphic study based on 174 proven cases.


The pathology of 3-Nitro-4-hydroxyphanylarsonic acid toxicity in turkeys.


The phosphorylation sites of troponin I from white skeletal muscle of the rabbit.


The preparation and properties of pyruvate kinase from yeast. A new method is described for the preparation of pyruvate kinase from yeast. This eliminates proteolysis during the preparation. The molecular weight of yeast pyruvate kinase is 215000, and it is composed of four subunits. Such properties of the enzyme as its extinction coefficient, cold-lability, thiol-group reactivity and binding of Mn(2+) ions are compared with those previously reported for yeast pyruvate kinase prepared by different methods. The specific activity is significantly higher than previously observed, but otherwise the enzyme is similar, apart from its molecular weight and Mn(2+)-binding characteristics, to preparations from Saccharomyces cerevisiae obtained in this laboratory (e.g. Fell et al., 1972, and references therein) and that of C. H. Suelter (e.g. Kuczenski & Suelter, 1971, and references therein), and is different from the enzyme isolated from Saccharomyces carlsbergensis by B. Hess and his co-workers (e.g. Wieker & Hess, 1972, and references therein).


The primary structure of apolopoprotein-serine.


The production of limit-oligonucleotide digests from phenylalanine tRNA (Escherichia coli) with snake venom phosphodiesterase.


The purification of cell nuclei from calf uterus by zonal centrifugation in reorienting density gradients.


The production of superoxide radical during the decomposition of potassium peroxochromate(V).


The psychogeriatric patient in hospital. The records of 148 geriatric patients discharged from the Royal Ottawa Hospital over an 18-month period were studied. The readmission rate, 38%, was similar to that of all inpatients in this hospital. The largest diagnostic category was depressive neurosis. More women than men had diagnoses of affective disorders, while more men had organic brain disease; these differences were statistically significant. The average length of hospital stay was 25 days. Those patients with diagnoses of organic brain disease and alcoholism had shorter stays than those with affective disorders. The implications of these findings in terms of setting up a psychogeriatric unit are discussed.


The pH-dependence of ESR spectra from nitroxide probes in lecithin dispersions.


The rate of cholesterol 'flip-flop' in lipid bilayers and its relation to membrane sterol pools.


The rate-limiting step of adrenal steroidogenesis and adenosine 3':5'-monophosphate.


The regulation of calciferol-25-hydroxylase in the chick.


The regulatory center of D-glyceraldehyde-3-phosphate dehydrogenase.


The renin-angiotensin system in acute renal failure in rats.


The renin-angiotensin system of uninephrectomized rats under diuretic treatment.


The respiratory system of the marine bacterium Beneckea natriegens. Oxidation--reduction potentials of the cytochromes.


The role of cyclic AMP in the modulation of cardiac contractility.


The role of prolactin in the depressed or 'buffered' adrenocorticosteroid response of the rat.


The role of cyclic AMP in the regulation of steroid biosynthesis in testis tissue.


The role of sialic acid in determining the survival of transcortin in the circulation.


The sensitivity of function tests in detecting liver damage in the rat.


The specificity of dimethylbenzylrifampicin as an inhibitor of viral induced transformation. The effect of 2',6'-dimethyl-N(4')-benzyl-N(4')- [desmethyl]rifampicin on viral transformation induced by two unrelated oncogenic viruses was compared. Transformation of Balb/3T3 cells by the small, DNA-containing papova virus simian virus 40 was completely normal under conditions where transformation by the large, RNA-containing oncornavirus murine sarcoma virus was inhibited more than 150-fold. For these experiments a resistant variant of Balb/3T3 was selected that grows well in high concentrations of the drug, is not dependent on the drug for growth, and is probably not blocked at the level of drug uptake. These data show that dimethyl-benzylrifampicin specifically inhibits oncornavirus-induced transformation rather than nonspecifically inhibiting cellular growth or transformation.


The sodium-potassium adenosinetriphosphatase.


The stability of PGM and AK isoenzymes in human tissues.


The stoichiometry of the three different types of copper in ascorbate oxidase from green zucchini squash.


The structure of cytochrome oxidase membranes.


The structure of the posterior piriform cortex in the dog.


The surface structure of polyoma virus.

The surface structure of polyoma virus.


The subfractionation of platelet membranes by zonal centrifugation: identification of surface membranes.


The synthesis of encephalomyocarditis virus polypeptides in infected L-cells and cell-free systems.


The treatment of muscular subaortic stenosis.


The ultrastructure of cartilage cells in the epiphyses of long bones in the domestic fowl.


The temperature-dependent enzymatic breakdown of ribosomal RNA of reticulocytes. The nucleoside plus mononucleotide fraction.


The ultrastructure of Dentalium egg at the trefoil stage.


The use of red light for handling wild rats.


The use of deoxyfluoro-D-galactopyranoses in a study of yeast galactokinase specificity. 1. 2-Deoxy-2-fluoro-d-galactose, 3-deoxy-3-fluoro-d-galactose, 4-deoxy-4-fluoro-d-galactose, 6-deoxy-6-fluoro-d-galactose and 2-deoxy-d-lyxo-hexose are substrates for yeast galactokinase. 2. The variation in K(m) values for the d-hexose derivatives was not associated with a variation in the value of K(m) for MgATP(2-) indicating that the binding of MgATP(2-) is not modified by the binding of the sugar substrate. 3. Donated H bonds from OH-3, OH-4 and OH-6 and an accepted H bond to OH-2 of the d-hexose are important for the binding of the sugar substrate to galactokinase. 4. Yeast galactokinase exhibits similar kinetics to the galactokinase from Escherichia coli and operates by a similar random sequential mechanism. 5. 4-Deoxy-4-fluoro-d-glucose was neither a substrate for nor an inhibitor of yeast galactokinase.


The theme is change.

The theme is change.


The RNA of defective vesicular stomatitis virus particles in relation to viral cistrons.


The SV40-induced T-antigen in mouse cells.


Theory of radioimmunoassays and hormone-receptor interactions. II. Simulation of antibody divalency, cooperativity and allosteric effects.


Thermography of the eye during cold stress.


The use of ribosubstitution techniques for determining DNA sequences.


Theory of hormone-receptor interaction. 3. The endocrine target cell as a quantal response unit: a general control mechanism.


Thymic lymphocytes in obese (ob-ob) mice. A mirror of the insulin receptor defect in liver and fat.


Thymus graft in four children: clinical and immunological results.


Thyroid lymphography.

Thyroid lymphography.


Tissue differences in the concentration of triiodothyronine nuclear binding sites in the rat: liver, kidney, pituitary, heart, brain, spleen, and testis.


Thyroid function following partial thyroidectomy.


Toxic effects of anticonvulsants: general principles.


Toxic properties of chlorine trifluoride.


Toxicity studies on tetrasul. 3. Short-term comparative studies in rats with tetrasul and structurally related acaricides.


Toxicity studies on tertrasul. II. Short-term comparative studies in 6 animal species.


Toxic polyneuropathy due to glue sniffing. Four cases of toxic polyneuropathy due to glue-sniffing were reported. Neurological examination revealed motor predominant mixed type polyneuropathy. The cause of polyneuropathies in these cases was considered to be due to the inhalation of the vaporized elements of the adhesive agent, which contain mainly n-hexane and toluene. N-hexane is considered to be chiefly responsible for the polyneuropathy, though toluene also could have some influence on the illness.


Transcortin: a corticosteroid-binding protein of plasma. XII. Immunologic studies on transcortin in guinea-pig tissues.


Transfection of chicken embryo cells with DNA extracted from avian virus-producing neoplastic cells. DNA isolated from avian virus-producing leukemic myeloblasts induced the production of viruses, but not morphological transformation, in cultivated chicken fibroblasts. The recovered virus had the same biological characteristics as the original avian myeloblastosis virus (AMV) and produced myeloblastosis and nephroblastomas when injected into chickens. Neutralization experiments with chicken anti-AMV-BAI strain A sera showed an antigenic community between the DNA-transfected virus and the original virus. Virus induced in fibroblasts after treatment with DNA from a viral nephroblastic nephroblastoma line only gave nephroblastoma when injected into chicken. Treatment of chicken embryo cells with DNA extracted from normal chicken embryos did not induce viral production.


Transcribing complexes in cells infected by vesicular stomatitis virus and rabies virus. We analyzed cell extracts from BHK(21) cells infected with vesicular stomatitis virus (VSV) and rabies virus for in vitro RNA polymerase activity. Cells infected with VSV B virions exhibited several complexes with in vitro RNA polymerase activity in sucrose gradients. These complexes synthesize VSV transcriptase product (4 to 18S) polyadenylated in RNA complementary to virion RNA. Cells infected with a high multiplicity of B virions and T particles show only one RNA polymerase complex active in vitro. This complex sediments at 110S and makes only small (2S) RNA. Carrier BHK(21) cells persistently noncytopathically infected with VSV contain several complexes active in RNA polymerase, but both exhibit very low activity. Cytoplasms of cells noncytopathically infected with rabies virus also show very low levels of a complex containing RNA polymerase activity. No transcriptase nor any other in vitro polymerase activity could be found associated with purified rabies virions, although they do carry out primary transcription in cells treated with actinomycin D and cycloheximide before infection.


Transfer of very low density lipoprotein from hen plasma into egg yolk.


Transient monoclonal gammopathy associated with cytomegalovirus infection.


Treatment of a case of sympathetic ophthalmia.


Translation of reovirus mRNA, poliovirus RNA and bacteriophage Qbeta RNA in cell-free extracts of mammalian cells.


Treatment of retinoblastoma.

Treatment of retinoblastoma.


Treatment of hyperlipoproteinaemia type II A with a new anion exchange resin secholex.


Treatment of stabilized liver cirrhosis by dehydroepiandrosterone sulphate. I. Evaluation of principal biochemical tests as a whole.


Trehalose metabolism by Bacillus popilliae. Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose was found to undergo phosphoenolpyruvate (PEP)-dependent phosphorylation, and the resulting trehalose 6-phosphate was cleaved by a phosphotrehalase to equimolar amounts of glucose and glucose 6-phosphate. The phosphotrehalase was purified 34-fold and shown to have a pH optimum of 6.5 to 7.0 and a K(m) for trehalose 6-phosphate of 1.8 mM. A mutant missing the phosphotrehalase failed to grow on trehalose but grew normally on other sugars. The mutant accumulated [(14)C]trehalose as [(14)C]trehalose 6-phosphate. Phosphorylation of trehalose by dialyzed extracts was at least 25 times faster with PEP than with adenosine 5'-triphosphate, and the phosphorylation activity was associated primarily with the particulate fraction. These data and the results of studies of [(14)C]trehalose uptake suggest that trehalose is transported into the cell as trehalose 6-phosphate by a PEP:sugar phosphotransferase system. Cell extracts of other strains of B. popilliae were also found to produce [(14)C]sugar phosphate from [(14)C]trehalose and to have phosphotrehalase activity.


Trisomy-9 in the bone marrow of a patient with acute myelomonoblastic leukaemia.


Tritium sequence analysis of polyribonucleotides following periodate-phosphomonoesterase degradation--analysis of nucleoside methylene dialdehydes derived from tetra-, penta-, and hexanucleotides.


Tumor-specific membrane antigens in established cell lines from gliomas.


Tumors in Gerbillinae: a literature review and report of a case.


Type 2 hyperprolinemia: absence of delta1-pyrroline-5-carboxylic acid dehydrogenase activity. triangle up(1)-Pyrroline-5-carboxylic acid dehydrogenase activity was measured radioisotopically in normal and type 2 hyperprolinemia fibroblasts. The type 2 cells had no detectable activity over a range of reaction conditions whereas normal cells had easily measurable activity. This enzymatic defect accounts for the biochemical abnormalities in type 2 hyperprolinemia.


TSH initiation of hormone secretion by rat thyroid lobes in vitro.


Ultrasonic diagnosis in melanoma and retinoblastoma.


Ultrastructural changes produced in Proteus vulgaris by a synergistic combination of colistin and sulphadiazine.


Ultrastructural examination of various stages of amelogenesis in the rat following parenteral fluoride administration.


Ultrastructural alterations of rat brain microglial cells and pericytes after chronic lead poisoning.


Ultrastructural observations of anterior pituitary somatotrophs following pituitary portal vessel infusion of dibutyryl cAMP.


Ultrastructural studies on tumours and cell cultures of the Harding--Passey mouse melanoma.


Ultrastructural study of brain lesions produced in mice by the administration of Clostridium welchii type D toxin.


Ultrastructural study of androgen-producing adrenocortical adenoma.


Ultrastructure of polymorphic Mucor as observed by means of freeze-etching. II. Vegetative yeast form grown under anaerobic conditions.


Ultrastructure of the chamber angle after a short period of high intraocular pressure.


Ultrastructure of polymorphic Mucor as observed by means of freeze-etching. 3. Dormant sporangiospore.


Ultrastructure of the extended ribonucleic acid molecules from purified ribosomes of Rous sarcoma virus-induced mouse ascites sarcoma cells.


Ultraviolet-enhanced reactivation of Herpes virus in human tumor cells.


Untreated neonatal herpes simplex 2 meningitis without apparent neurologic damage.


Ultrastructure of pure cultures of Physarum flavicomum. 1. Conversion of a plasmodium to microplasmodia and microsclerotia, and the process of slime secretion.


Urinary excretion and cerebrospinal fluid concentration of cyclic adenosine-3',5'-monophosphate in various neurological diseases.


Use of benzoylated cellulose columns for the isolation of poly(adenylic acid) containing RNA and other polynucleotides with little secondary structure.


Use of immune lymphocytes to detect expression of herpetic genome.


Utilization of ascites plasma very low density lipoprotein triglycerides by Ehrlich cells. Much of the lipid present in the ascites plasma in which Ehrlich cells grow is contained in very low density lipoproteins (VLDL). Chemical measurements indicated that triglycerides were taken up by the cells during in vitro incubation with ascites VLDL. When tracer amounts of radioactive triolein were incorporated into the ascites VLDL, the percentage uptakes of glyceryl tri[1-(14)C]oleate and triglycerides measured chemically were similar. The cells also took up [2-(3)H]glyceryl trioleate that was added to VLDL, but the percentage of available (3)H recovered in the cell lipids was 30-40% less than that of (1 4)C from glyceryl tri[1-(1 4)C]oleate. This difference was accounted for by water-soluble (3)H that accumulated in the incubation medium, suggesting that extensive hydrolysis accompanied the uptake of VLDL triglycerides. Radioactive fatty acids derived from the VLDL triglycerides were incorporated into cell phospholipids, glycerides, and free fatty acids, and they also were oxidized to CO(2). Triglyceride utilization increased as the VLDL concentration was raised. These results suggest that one function of the ascites plasma VLDL may be to supply fatty acid to the Ehrlich cells and that the availability of fatty acid to this tumor is determined in part by the ascites plasma VLDL concentration. Although Ehrlich cells incorporate almost no free glycerol into triglycerides, considerable amounts of [2-(3)H]glyceryl trioleate radioactivity were recovered in cell triglycerides. This indicates that at least some VLDL triglycerides were taken up intact. The net uptake of VLDL protein and cholesterol was very small relative to the triglyceride uptake, suggesting that intact triglycerides are transferred from the ascites VLDL to the Ehrlich cells and that hydrolysis occurs after the triglyceride is associated with the cells.


Uveitis, episcleritis and conjunctivitis associated with Yersinia infection.


Vacuolar transcellular channels as a drainage pathway for cerebrospinal fluid. 1. Based on our ultrastructural investigations in monkeys, we report here a new concept as to the physiological mechanism of drainage of cerebrospinal fluid (c.s.f.) which would seem to bridge the gap between the two apparently opposing views of ;closed' and ;open' system.2. Our studies reveal the presence of an intact mesothelial lining of the arachnoid mater, including its villus-like projections and herniations into the dural sinus and its lacunae, adjacent cells being joined by tight junctions; in addition we have observed for the first time that many lining cells in the region of the superior sagittal sinus are characterized by unit membrane-bound, electron-optically empty giant vacuoles of several micrometres diameter. In one monkey with a fresh subarachnoid haemorrhage, many vacuoles were filled with plasma proteins and some contained intact blood corpuscles.3. Serial section analysis showed that the vacuoles were in fact invaginations from the basal aspect of the cell surface and were evidently in direct communication with the c.s.f. in the subarachnoid space. Some vacuoles in addition showed openings on their apical surface thus constituting transcellular channels or pores. Basal openings up to 3.5 mum and apical openings up to 2.3 mum were seen.4. It is postulated that vacuoles are stages in the formation of a dynamic system of transcellular pores which allow the bulk outflow of c.s.f. down a pressure gradient, and that the mesothelial vacuolation cycle, in providing the requisite number of transcellular pores across the mesothelial barrier at any time, is a controlling factor in the outflow of c.s.f. and in the maintenance of its fluid-pressure within the subarachnoid space.5. The basic similarity between the bulk flow of the aqueous humour and c.s.f. from the anatomically closed cavities of the anterior chamber and the subarachnoid space, respectively, is underlined.6. The present study provides further support for our hypothesis that the bulk outflow of fluid, via a dynamic system of transcellular pores formed by gradually enlarging membranous surface infoldings in a single cell, termed as giant vacuoles, is a fundamental biological process not hitherto described.


Variability of sleep measures in normal subjects.


Unusual metastatic sites of colonic and rectal carcinoma: Report of four cases.


Value of aortography in the diagnosis of congenital multicystic kidney.


Vascular intimal components: precursors of thrombosis.


Vascular responsiveness of the in situ perfused dog pancreas.


Ventilation and sleep state in the new-born. 1. Ventilation, oesophageal pressure and sleep state were measured in fourteen babies between 25 hr and 7 days of age.2. Trunk plethysmograph records showed that respiratory rate and minute volume were significantly higher in REM sleep than non-REM sleep. Tidal volume was not significantly altered.3. Tidal volume, respiratory rate and minute volume were all more variable in each baby in REM sleep, both on breath to breath analysis and over successive 20 sec periods.4. Major oesophageal peristaltic activity was observed in REM sleep only.5. In response to a sharp rise in P(O2) the tidal volume and minute volume fell significantly by the same proportion in both sleep states. Respiratory rate did not change significantly.


Viability of swine vesicular disease in carcases and faeces.


Vascular characteristics of unilateral acute renal failure in the dog. Assessment with vasodilators and antagonists to angiotensin and norepinephrine.


Vasoactive intestinal peptide stimulation of adenylate cyclase and active electrolyte secretion in intestinal mucosa. Vasoactive intestinal peptide (VIP), originally isolated from hog small intestinal mucosa, has been shown to cause small intestinal secretion. More recently, this peptide has been identified in the plasma and tumors of patients with the so-called "pancreatic cholera" syndrome. In order to explore the possible role of VIP in the pathogenesis of this syndrome, we examined the effects of this peptide and other hormones on the cyclic AMP levels, adenylate cyclase activity, and ion transport in in vitro preparations of ileal mucosa. In rabbit ileal mucosa, VIP (20 mug/ml) caused a prompt fivefold increase in cyclic AMP level, whereas nine other hormones, which have been postulated to cause intestinal secretion, failed to exert such an effect. Pentagastrin and glucagon also failed to increase cyclic AMP levels in canine ileal mucosa. An increase in mucosal cyclic AMP levels was observed at a VIP concentration of 0.1 mug/ml and appeared to be nearly maximal at 2.0 mug/ml. VIP (100 mug/ml) stimulated adenylate cyclase activity in a membrane preparation from rabbit ileal mucosa. Secretin (6.0 x 10(-5) M) failed to do so. When added to the serosal side of isolated rabbit ileal mucosa clamped in an Ussing chamber, VIP (2 mug/ml) increased short-circuit current (SCC) and caused net secretion of both Cl and Na. Net Cl secretion exceeded net Na secretion. These effects of VIP on mucosal cyclic AMP metabolism and ion transport are similar to those observed with cholera enterotoxin and certain prostaglandins. VIP was also tested with normal human ileal mucosa. At a concentration of 2 mug/ml it caused a fivefold increase in cyclic AMP level and an increase in SCC of the same magnitude as that caused by 5 mM theophylline. Addition of a second 2-mug/ml dose of VIP and addition of theophylline after VIP produced no further change in SCC. We conclude the VIP stimulates adenylate cyclase and active ion secretion in both rabbit and human ileal mucosa. This may be related to the pathogenesis of diarrhea in patients with the pancreatic cholera syndrome.


Villous adenoma of the colon and rectum: an ultrastructural study. The villous component of nine colonic polyps (three pure villous adenoma and six mixed adenomatous and villous adenomas) was studied by light and electron microscopy. Normal colonic mucosa was used for control. The predominant cell was an undifferentiated epithelial cell resembling those of the base of the crypts of normal colonic mucosa. A minority of these cells differentiated toward goblet cells and absorptive cells. Interdigitations of the plasma membrane with enlarged intercellular spaces were frequently observed, possibly related to the loss of water and electrolytes in some of these tumors, suggesting an analogy to renal tubular plasma membrane changes in kalliopenic nephropathy. Numerous membrane-bound, inter and intracellular "inclusion bodies" representing degenerated lymphocytes and epithelial cell organelles were commonly found. Viral inclusions were not detected in this material. The possibility that the inclusion bodies observed in villous adenoma may represent lysosome-modified vesicles, secondary to absorption of protein and surface mucosubstances appears unlikely.


Virus infection and incidence of respiratory disease: a serological study of four micro-organisms at a large beef-rearing farm.


Virus-specific proteins associated with ribosomes of Krebs-II cells infected with encephalomyocarditis virus.


Viruses and the immune response.

Viruses and the immune response.


Viral teratology.

Viral teratology.


Vitamin D hormones. 2.

Vitamin D hormones. 2.


Vitamin D hormones: 1.

Vitamin D hormones: 1.


Water pollution. Microbiology--detection, occurrence, and removal of viruses.


What is analgesia?

What is analgesia?


Wilms's tumour--an improved prognosis. Report of 22 consecutive children seen from 1967-71.


X-ray small angle scattering of human plasma high density lipoprotein LpA from HDL2: application of a new evaluation method.


X-ray diffraction studies of DNA at reduced water contents.


1,N6-etheno-2-aza-adenosine 3',5'-monophosphate: a new fluorescent substrate for cycle nucleotide phosphodiesterase.


Xanthine oxidase deficiency: studies of a previously unreported case.


1H nuclear magnetic resonance studies of thyrotropin releasing factor (TRF).


2',3'-Cyclic nucleotide 3'-phosphohydrolase activity in human brain tumours.


270 MHz proton magnetic resonance spectra of metallothionein.


3' End-group labeling and partial sequence determination of oligodeoxynucleotides.


5'-Terminal nucleotide sequences of the double-stranded RNA of silkworm cytoplasmic polyhedrosis virus.


6-O-methyl-D-glucosamine in lipopolysaccharides of Rhodopseudomonas palustris strains.


8 alpha-substituted flavins of biological importance.


On the hypothalamic regulation of the adenohypophysial gonadotropic function.


Probable sites for estrogen receptors in brain and pituitary.


Symptomatology of early maturing girls.

Symptomatology of early maturing girls.


(R,S)-2,3-epoxypropyl ethers and glycosides of D-glucopyranose.


c-AMP phosphodiesterase activity during growth and differentiation in Blastocladiella emersonii.


p-Nitrophenylphosphatase in denervated muscle.


[Diagnosis, surgical and radiotherapeutic treatment of malignant tumors of the upper jaw].


[Mixed mesodermal tumor of the uterine corpus in a 4-year-old girl].


A case of atrial myxoma.

A case of atrial myxoma.


A comparative study of some properties of chromatin from normal diploid and SV-40 transformed human fibroblasts.


A column procedure for the esterification of organic acids with diazomethane at the microgram level.


A comparison of renin and erythropoietin levels in anephric man.


A cytogenetic investigation of blood lymphocytes in chronic forms of virus hepatitis.


A direct, stimulating effect of cyclic GMP on purified phosphoribosyl pyrophosphate synthetase and its antagonism by cyclic AMP.


A fluorescent method for the demonstration of cyclic 3',5'-AMP phosphodiesterase activity in polyacrylamide gel.


A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: testosterone production by dispersed Leydig cells.