Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis. The proteins of simian virus 40 (SV40) and two human papovaviruses, the hemagglutinating BK virus and the non-hemagglutinating DAR virus, were analyzed and compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The virions of SV40 and DAR contain seven proteins. By molecular weight analysis the constituent proteins of SV40 and DAR are identical. Approximately 84% of the viral protein has a molceular weight of 45,000. The major protein of BK virus is 3,000 to 5,000 daltons lighter than the major proteins of SV40 and DAR viruses. The five most rapidly migrating proteins of BK virus are indistinguishable by molecular weight analysis from the corresponding proteins of SV40 and DAR viruses. Radial immunodiffusion and immunoelectrophoresis of whole virus gave lines of identity between SV40 and DAR when reacted with SV40 antibody. SV40 antiserum tested against BK virus and BK antiserum tested against SV40 virus showed no reactivity by complement fixation, immunodiffusion, or immunoelectrophoresis.


Effect of ionic strength on the binding of Sindbis virus to chick cells. Sindbis virus can adsorb to chicken embryo fibroblasts in two different ways. "Loosely" bound virus can be washed off the cell with buffers of ionic strength 0.2 or greater, whereas "tightly" bound virus remains attached under these conditions. When Sindbis virus is adsorbed to chick cells at 4 C from a buffer of ionic strength 0.17, 40 to 50% of the adsorbed virus is loosely bound, the remainder tightly bound. Infection of chick cells by Sindbis virus has only small effects on the total amount of virus that can be bound to the cells. However, the amount of Sindbis virus that can be tightly bound declines rapidly beginning at 2 to 3 h after infection. By 7 h after infection, the amount of virus that can be tightly bound is only 10 to 20% of the amount bound to uninfected cells. The adsorption (and penetration) of virus at 37 C is most efficient at an ionic strength of 0.15 to 0.17; at this ionic strength most of the adsorbed virus is tightly bound. At higher ionic strengths the virus adsorbs poorly. At lower ionic strengths most of the virus is loosely bound. A second enveloped virus, vesicular stomatitis virus, has been studied for the purposes of comparison; its adsorption behavior differs from that of Sindbis virus.


Analysis of a viral agent isolated from multiple sclerosis brain tissue: characterization as a parainfluenzavirus type 1. A virus originally isolated from cell cultures obtained by lysolecithin-induced fusion of human multiple sclerosis brain cells with CV-1 cells has been analyzed for its antigenic, RNA, and polypeptide compositions, and for selective biological properties. Our findings establish that this isolate, designated 6/94 virus, contains a 50S RNA genome and is, as yet, indistinguishable from Sendai virus in its antigenic and total polypeptide compositions. Despite these similarities, the 6/94 and Sendai viruses differ in certain phenotypic properties. 6/94 virus is markedly less cytocidal for chick fibroblasts, especially at 37 C and, after beta-propiolactone inactivation, it possesses a greater capacity for cell fusion and a lower toxicity than does comparably treated Sendai virus. In addition, 6/94 virus shows greater hemolytic activity.


Location of the origin of DNA replication in adenovirus Type 2. Utilizing the isolated left and right halves of both adenovirus type 2 and the nondefective adenovirus simian virus 40 hybrid (Ad2(+)ND(1)), studies were undertaken to find the site on the DNA molecules at which replication begins. The data are consistent with several models which include an initiation event at both ends and bidirectional growth.


Polyadenylation of vesicular stomatitis virus mRNA. Vesicular stomatitis virus (VSV) mRNA isolated from infected cell polysomes contains polyadenylic acid [poly(A)] sequences. Detergent-activated purified virions in vitro can transcribe complementary RNA, which has sedimentation properties similar to mRNA, and this RNA also contains poly(A) sequences. Digestion of virion RNA with U2 RNase under conditions where hydrolysis is specific for purine linkages leaves no sequences of polyuridylic acid corresponding in length to the poly(A) on the transcripts. Growth of infectious virus is not inhibited by 3-deoxyadenosine (cordycepin) under conditions in which it inhibits polyadenylation of cellular mRNA. The virus-specific mRNA produced in the presence of cordycepin has poly(A) sequences of the same size distribution as that synthesized in the absence of cordycepin.


Complementation analysis of simian virus 40 mutants. Seventy six new temperature-sensitive mutants of simian virus 40 have been isolated. A simple modification of the standard plaquing technique permits complementation analyses to be performed readily. By using this technique the new mutants have been divided into four complementation groups.


Poliovirus infection and poly(A) sequences of cytoplasmic cellular RNA. The influence of polio infection on Poly(A) sequences of cellular cytoplasmic RNA was investigated. In the presence of guanidine, cellular protein synthesis was still shut off after poliovirus infection, although there was no viral RNA synthesis. The Poly(A) of these cells was unchanged with respect to quantity, size, and linkage to cellular cytoplasmic RNA. This finding strongly suggests that the shut off of cellular protein synthesis is not caused by a change of the Poly(A) sequences of cellular mRNA.


Separation of B and C type virions by centrifugation in gentle density gradients. Murine type B particles were separated from type C (Rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. The best separation was obtained when the density ranged from 1.13 to 1.20 g/cm(3) when sucrose was used and from 1.12 to 1.28 g/cm(3) with CsCl. The buoyant densities of the B and C particle bands in sucrose were 1.18 and 1.16 g/cm(3), respectively. The CsCl gradient gave a better separation with the B particles banding at a density of 1.20 g/cm(3) and with the C particle density little different from its value in sucrose.


Isolation and partial characterization of different defective DNA molecules derived from polyoma virus. Supercoiled DNA molecules purified from mouse cells infected with high-multiplicity-passaged polyoma virus has a broader size distribution and sediments more slowly than DNA derived from low-multiplicity-passaged virus. The shorter DNA molecules are predominately noninfectious. Virus populations containing distinct size classes of defective virus DNA were isolated by growing virus from single cells infected by a defective and nondefective helper virus (infectious center). This technique probably results in the cloning of defective virus particles. By applying the infectious center method to DNA from various fractions of sucrose gradients it has been possible to obtain shorter circular DNA molecules ranging in size from 50 to 95% of the unit-length polyoma DNA molecule. The shorter molecules in any one preparation are homogeneous in size. This class size is retained upon repeated passage of crude viral lysates at high multiplicity. Thus far, all the purified shorter DNA molecules tested appear to be noninfectious and largely resistant to cleavage by the R(1) restriction enzyme. Some of the purified defective molecules have been found to interfere with the production of infectious virus upon co-infection with unit-length infectious polyoma DNA.


Surface membrane glycopeptides which coincide with virus transformation and tumorigenesis. Glycopeptides from the surface of clones of hamster embryo cells were examined at various intervals after infection with polyoma virus. Two types of transformed cells were examined: (i) clones that showed delayed transformation or an initially low tumorigenicity, and (ii) clones that were rapidly transformed showing an initially high tumorigenicity. The glycopeptides were removed from the cell surface by trypsin and, after Pronase digestion, were examined by filtration through Sephadex G-50. With delayed transformation, a specific group of glycopeptides was increasingly evident over an 85-day period as the cells showed phenotypic properties of transformation and the ability to form tumors. In the other series, all but one clone of hamster embryo cells showed rapid transformation after infection with polyoma virus. This clone was less tumorigenic and showed little of the specific glycopeptides. In all cases of delayed or rapid transformation examined, the specific group of glycopeptides increased proportionately to the ability of the cells to form tumors. All of the cells derived from progressively growing tumors formed by injection of these transformed hamster cells into adult animals showed an abundance of this group of glycopeptides. These results suggest that specific surface membrane glycopeptides accompany viral transformation and tumorigenesis.


Toward control of tumour and virus growth by helical RNA. II. Difference in the post-integrational control of virus synthesis by Poly I:C in acutely and chronically B77 virus infected cells.


Possiblity of cancer diagnosis by detection of Clostridial antibodies.


Lesions in sheep skeletal and oesophageal muscle in vermeersiekte (Geigeria ornativa O. Hoffm. poisoning).


Congenital mesoblastic nephroma of infancy.


Cell surface receptors--structure and function.


Platelet function in health and disease.


Origin and formation of lymph lipids in the sheep.


Amniogenic bands as a cause of syndactyly in a young human fetus.


Calcification of oral bacteria: an ultrastructural study of two strains of Bacterionema matruchotii.


A note on fine structure of synovial sarcoma.


An autopsy case of primary cytomegalic inclusion enteritis with remarkable hypoproteinemia.


Studies on animal collagenase.

Studies on animal collagenase.


On the turnover of acetylcholine in the brain. An experimental study using intravenously injected radioactive choline.


Mucous substance and enzyme histochemistry of non-neoplastic and neoplastic gastric epithelium in man.


Some current concepts and concerns relative to amebae and disease in man.


Virulence of axenically cultivated E. histolytica.


Studies on antibody surveillance to EB virus-induced antigen in patients with sarcoidosis and nasopharyngeal carcinoma by indirect immunofluorescence.


Aberrant forms of varicella-zoster virus.


Control of flagellum formation in Naegleria gruberi.


Metabolic pathways and control mechanisms involved in the biosynthesis and turnover of collagen in normal and pathological connective tissues.


Electron optic analysis of human dentin in hypophosphatemic vitamin D-resistant rickets (report of a kindred with consanguinity).


Collagen metabolism in pericoronitis.

Collagen metabolism in pericoronitis.


Electron-phonon (trapped photon) coupling and infrared coaxial transmission line theory of energy transport in mitochondria and nerve.


Current therapy approaches of the Division of Cancer Treatment with emphasis on pancreatic carcinoma.


A study of the mechanism of the formation of the unproductive lactate dehydrogenase-NAD-pyruvate ternary complex.


Fine structure of the magnetooptical rotatory dispersion curves and the surroundings of heme in ferrocytochrome c and its model compounds.


Conformational analysis of globular proteins by optical rotatory dispersion.


Proceedings: 155. Role of brain biogenic amines in the regulation of ACTH secretion in the rat.


In vitro studies on the nature of prostaglandin E1 binding in the hamster uterus.


Proceedings: A new rapid method of enzymic analysis using rate reaction techniques.


Inhibition of 3',5'-cyclic AMP phosphodiesterase by acid mucopolysaccharides and sulfopolysaccharides.


Inhibition of 3',5'-cyclic AMP phosphodiesterase with anti-inflammatory agents.


Enhancement of the hepatotoxicity of trichloroethylene by inducers of drug metabolism.


The effect of apyrase on platelet retention in normal subjects and in patients with abnormal cardiac valves or with von Willebrand's disease.


Effects of prostaglandins E1 and E2 on renal sodium reabsorption and Starling forces.


Experimental observations on the effects of physical training upon intrinsic cardiac physiology and biochemistry.


Fever produced in the squirrel monkey by intravenous and intracerebral endotoxin.


Brush-border processes and transepithelial Na and Cl transport by rabbit ileum.


Effects of ADH and other agents on cyclic AMP accumulation in toad bladder epithelium.


Effects of hormones on cyclic AMP formation in kidneys of nonmammalian vertebrates.


Dibutyryl cyclic AMP and potassium transport in cardiac Purkinje fibers.


Enumeration of food-borne Clostridium perfringens in egg yolk-free tryptose-sulfite-cycloserine agar. The SFP (Shahidi-Ferguson perfringens), TSC (tryptose-sulfite-cycloserine), EY (egg yolk)-free TSC, and OPSP (oleandomycin-polymyxin-sulfadiazine perfringens) agars have been tested for their suitability to enumerate Clostridium perfringens in naturally contaminated foods. Complete recoveries of C. perfringens were obtained in each of the four media, but only the TSC and EY-free TSC agars were sufficiently selective to ensure subsequent confirmatory tests without interference from facultative anaerobes. Because of some disadvantages associated with the use of egg yolk, EY-free TSC agar is recommended for enumeration of C. perfringens in foods. Several conditions for convenient shipment of foods and C. perfringens isolates with minimum loss of viability have been tested. The highest viable counts were preserved when foods were mixed 1:1 (wt/vol) with 20% glycerol and kept in a container with dry ice. Isolated C. perfringens strains remained viable for at least 2 weeks at ambient temperatures on blood agar slopes with a 2% agar overlay in screw-cap culture tubes.


Recovery of poliovirus from turbid estuarine water on microporous filters by the use of celite. The application of a new step for recovering poliovirus from moderately to highly turbid estuarine water by the filter virus-adsorption technique was investigated. The experiments were conducted under both (i) laboratory-based conditions (200-ml volumes) where the turbidity was controlled and (ii) simulated field conditions (15- to 100-gal volumes) where the turbidity varied depending upon the hydrology of the raw estuarine water. The new step consisted of adding Celite to the turbid water prior to sampling for virus. In the experiments, the pH of the water was first adjusted to 3.5 and then AlCl(3) was added to 0.0005 M. Celite was added to a concentration of 0.01% and mixed thoroughly. Either an HE Cox M-780 microfilter (Cox Instrument, Div. of Lynch Corp., Detroit, Mich.) or an MF-membrane filter (Millipore Corp., Bedford, Mass.) was used as the virus adsorbent. Virus was eluted from the Celite-filter complex in situ at pH 9 with 5x nutrient broth. In the laboratory-based experiments, when turbidity ranged from 5.0 to 30.0 Jackson turbidity units (JTU), virus recovery ranged from 66 to 89%. In the simulated field experiments, when the turbidity ranged from 8.5 to 80.0 JTU, virus recovery ranged from <1 to 74%, depending upon the multiplicity of virus input and the level of turbidity. The new step greatly improved the filtration-flux of turbid water and significantly reduced the premature clogging problem usually observed with microporous filters.


The amino acid sequence of a protein (apovitellenin I) from the low-density lipoprotein of emu egg yolk.


Enumeration of fecal Clostridium perfringens spores in egg yolk-free tryptose-sulfite-cycloserine agar. The Shahidi-Ferguson perfringens, tryptose-sulfite-cycloserine (TSC), and egg yolk-free TSC agars have been tested for their suitability to enumerate fecal spores of Clostridium perfringens. When these spores comprised at least 20% of the total anaerobe spores, equally accurate counts were obtained in the three media. With lower ratios of C. perfringens spores, the most accurate counts were obtained in egg yolk-free TSC agar. The median C. perfringens spore count of 60 normal fecal specimens was log 3.4/g. A nonmotile, sulfite- and nitrate-reducing Clostridium, not identifiable with any known clostridial species, was isolated from 14 out of 60 fecal specimans. It was not differentiated from C. perfringens in the nitrite motility test, but could be distinguished by its inability to liquefy gelatin.


Editorial: The heart during sleep.

Editorial: The heart during sleep.


Pathology of murine cytomegalovirus infection in newborn mice. Muscle, heart, and brown fat lesions. Newborn mice were inoculated intracerebrally with murine cytomegalovirus and studies were made of the pathological changes in the striate and cardiac muscle and brown fat. Widespread necrosis was seen in muscle and brown fat in the early stages of the infection. Necrotic lesions became calcified. By 56 days lesions were not resolved in the heart and brown fat but were completely resolved in skeletal muscle.


Clinical pharmacology as an approach to the study of biochemical sleep mechanisms: the action of L-dopa.


Retarded growth of rats by anticonvulsant drugs.


The polycyclic sleep-wake cycle in the cat: effects produced by sensorimotor rhythm conditioning.


Asymptomatic infection with EB virus.

Asymptomatic infection with EB virus. Evidence of asymptomatic EB virus infection was sought in healthy medical students and nurses following the observation that 1.4% possessed heterophile antibodies. The proportion of subjects with EB virus antibodies rose with age and there was evidence of recent infection in 19%. Smooth muscle antibodies were detected in 13 individuals and recent infection with EB virus was probable in six of these. Subclinical EB virus infections, in some cases associated with some disturbance of immunological function, may therefore be not uncommon in young adults.


A cyclic adenosine monophosphate link in the catecholamine enhancement of transmitter release at the neuromuscular junction. The frequency of miniature endplate potentials (mepps) in rat diaphragms was markedly increased by epinephrine and norepinephrine in preparations exposed to 15 mM K(+). The effect was rapid in onset but gradually declined during continued exposure to the catecholamines. N(6), O(2')-dibutyryl adenosine 3',5'-monophosphate (dibutyryl-cAMP) also caused transient frequency increases resembling in time-course those observed with catecholamines. Contrary to previous reports, catecholamines and dibutyryl-cAMP had little effect on mepp frequency in preparations not treated with K(+). Sustained increases with theophylline and decreases with adenosine were found in both K(+)-treated and untreated preparations. Analysis of the data obtained with catecholamines showed the intensity of the response to be a function of nerve terminal polarization. The inability of catecholamines and dibutyryl-cAMP to affect mepp frequency of untreated preparations argues against an obligatory role for cAMP in the neurosecretory mechanism. The findings are consistent with an action of catecholamines and cAMP in the regulation of transmitter release at fatigued preparations.


Metabolic effects of antibodies on platelets.


Pulmonary generation and peripheral uptake of endogenous angiotensin II in man.


Stimulation of adenosine 3',5'-cyclic monophosphate accumulation in anterior pituitary gland in vitro by purified growth hormone-releasing hormone.


Investigation of the accompaniment of calcium during active calcium transport from human erythrocyte ghosts. To determine whether a cell metabolite was involved in active calcium transport, the cell contents of human erythrocytes were subjected to high dilutions and the resultant ghosts were checked for their ability to actively transport calcium. It was found that the diluted erythrocyte ghosts did retain their capacity to actively transport calcium and that the characteristics of this transport process appeared to be unaltered by the high dilutions. Calcium analysis of the cell membrane and cell supernatant indicated that almost all of the calcium was lost from the cell solution rather than the cell membrane as active calcium transport proceeded. Therefore it appeared that calcium was able to cross the cell membrane without the aid of a cell metabolite. Investigations with layered erythrocytes indicated that the active transport of calcium was not assisted by centrifugation. Neither inorganic phosphate, pyrophosphate, nor an adenine nucleotide appeared to accompany calcium across the membrane as indicated by total phosphate and inorganic phosphate analysis and 260-nm readings of the deproteinized supernatant.


Evidence that acromegaly is not a hypothalamic disease.


In vitro demonstration of normal thyrotropin (TSH) responsiveness in thyroid tissue from patients with Graves' disease.


Antifracture efficacy of long-term estrogens for osteoporosis.


The Distinguished Lecture. The adrenal cortex: reflections, progress and speculations.


Cartilage collagen: substrate in soluble and fibrillar form for rheumatoid collagenase.


Development of chloroplasts in intact Atropa belladonna and in stem callus cultures during greening and leaf differentiation.


Paneth cell metaplasia in diseases of the colon and rectum.


Quantitative cytologic measurements in hepatomas.


Complement-fixing antibodies against cytomegalovirus in different parts of the world. A WHO cooperative study in which 27 laboratories participated showed that the distribution of cytomegalovirus antibodies in healthy blood donors varies widely, ranging from 40% in highly industrialized areas to 100% in developing countries. Climatic factors did not appear to play an important role in the spread of the virus.


Ecological studies on Culex tritaeniorhynchus as a vector of Japanese encephalitis. In the Republic of Korea, light-trap assessments and collections from cows and pigs after sunset were highly successful in monitoring the seasonal abundance of C. tritaeniorhynchus, the primary vector of Japanese encephalitis. The dates and duration of the population peaks at semirural suburbs of Seoul and Pusan were markedly different from those at a rural rice-growing site. The main breeding sites in these three areas were, respectively, swamps, marshes, and ricefields. The overall adult densities were the lowest at the rice-growing site where agricultural pesticides were extensively used. A short period of man-vector contact occurred at each study site at low densities when the natural population was at its peak. These vector monitoring techniques, coupled with information on antibodies in swine, represent a simple but valuable surveillance system.


Immunity to cancer. Naturally occurring tumours in domestic animals as models for research. 1. PREVENTIVE VACCINATION IS SUCCESSFULLY PRACTISED TODAY AGAINST TWO NEOPLASTIC DISEASES OF DOMESTIC ANIMALS: fibropapillomatosis of cattle and Marek's disease of chickens (a lymphoproliferative disease). Also it may soon be possible to immunize cats against lymphosarcoma. This memorandum describes these diseases and the immunological reactions involved. It also mentions a number of other tumours that could be used for immunological studies.The greatest advances in immunity have been made with the tumours caused by viruses. The killed papillomavirus vaccine used against bovine papillomatosis produces demonstrable antibodies against the virus. In the case of Marek's disease of chickens, which is due to a herpesvirus, a live virus vaccine is used. This does not prevent infection with virulent virus, but prevents the development of neoplasia. The mechanism by which the vaccine produces its effect is not yet known. Immunization with live and with killed vaccines has been successfully carried out experimentally against leukosis of chickens, which is caused by an oncornavirus. There is evidence that it will be possible to vaccinate cats against lymphosarcoma with non-living vaccine.Naturally occurring cancer in domestic animals parallels cancer in man more closely than does experimentally induced cancer in inbred laboratory animals; therefore immunological studies with the former are more likely to yield results relevant to the problem in man. Experimental cancer in rodents provides models that have the great advantages of uniformity and availability, and they cannot be replaced. However, models in domestic animals offer valuable supplementary systems for research aimed at elucidating the basic principles of immunity to cancer.


Limitations of immunofluorescence tests in the diagnosis of infectious mononucleosis. The relative value of heterophil agglutinins (HA) and of specific EBV antibodies in the diagnosis of infectious mononucleosis (IM) was assessed in 108 cases of the disease and in 280 controls. Among the 108 cases 93 were HA-positive by sheep cells in at least one of their sera, while 15 were HA-negative by the same test. Among the 280 controls false-positive HA tests were not encountered except in eight cases with the horse cell microtitre tests. With one of the two slide tests at least two false-positive tests and 12 false-negative tests were also found but these sera had low titres in microtitre tests. The HA life-span was found to be unexpectedly long in a few cases, sheep cell HA lasting up to 8 to 10 months and horse cell HA up to 21 to 23 months.Many false-positive tests may therefore not be true false-positives and may result from the persistence of HA following unrecognized mononucleosis months before. Virtually all cases of IM had (or developed) antibodies to Epstein-Barr virus, viral capsid antigen (EBV-VCA), whereas only half of the controls were EBV-VCA-positive. The comparative analysis of nonspecific and specific test results in mononucleosis allows the following conclusions: (1) horse cell microtitre tests and the monospot test are more sensitive than sheep cell microtitre tests and the monotest; (2) false-negative results are occasionally seen with the latter tests but not with the former; (3) more false-positive results, however, are probably seen with the former tests; and (4) specific EBV-IgM and EBV-EA antibody tests are useful in the diagnosis of selected borderline cases of mononucleosis.


Parathyroid carcinoma: location of pelvic metastases by parathyroid hormone assay. A metastasis from a functioning parathyroid carcinoma was located by PTH radioimmunoassay and selective venous catheterization. The site of the metastasis, verified at autopsy, was in the right side of the pelvis. This is the most distant reported location for metastatic parathyroid carcinoma. The patient's plasma immunoreactive PTH rose more than twofold in response to induced hypocalcemia. This suggests that relative hypocalcemia, induced therapeutically in such patients, may result in a higher chronic level of PTH secretion.


Address to Annual Meeting of the American Medical Women's Association.


Success or failure: women as medical school applicants.


Sex ratios in authorship and acknowledgments for medical journal articles.


An internal auditing system for improving the quality of medical care.


Familial hypercholesterolemia (one form of familial type II hyperlipoproteinemia). A study of its biochemical, genetic and clinical presentation in childhood. Primary hyperbetalipoproteinemia (type II hyperlipoproteinemia) is a common disorder associated with premature vascular disease. It is frequently due to genetic abnormalities, some of which are expressed in childhood. We have examined the manner in which that form of hyperbetalipoproteinemia known as familial hypercholesterolemia may be expressed in 236 children aged 1-19 born of 90 matings in which one parent had hyperbetalipoproteinemia of this variety and one parent did not.Two Gaussian populations were fitted to the distribution of both low density lipoprotein cholesterol (C(LDL)) and plasma cholesterol (C) in these children and a likelihood ratio test strongly favored a two over a one population model for both C(LDL) (X(2) = 18.41, P < 0.0005) and C (X(2) = 7.81, P < 0.025). 45% of the children were in the population identified as affected; their mean C(LDL) was 229. The remaining 55% were in the normal population with a mean C(LDL) of 110 which was indistinguishable from that of an unrelated control population, aged 1-19. On the basis of an assumed frequency of hyperbetalipoproteinemia in the general population of 5%, the Edwards' test indicated that a polygenic model of inheritance was highly unlikely (expected, 22%; observed, 45%). The segregation ratio obtained from the derived intersection between the two population curves (C(LDL), 164 mg/100 ml; C, 235 mg/100 ml) was 45/55 (abnormal/normal). The percentage of abnormal children in the first decade (52%) significantly exceeded that in the second (39%) (P < 0.01). The ratios (II/N) were 50/47 and 55/84 in the offspring of affected female and male parents, respectively (X(2) = 3.819, 0.05 < P < 0.10). Only 10% of hyperbetalipoproteinemic children were considered to have hyperglyceridemia. These children, frequently, but not invariably, had a parent with hyperglyceridemia in addition to hyperbetalipoproteinemia (P < 0.05). None of the affected children who were examined had ischemic heart disease (IHD) and 7% had tendon xanthomas. Half of the parents (mean age, 37.4 yr) who were examined had IHD and three-quarters had xanthomas. The data agree well with the hypothesis that hyperbetalipoproteinemia is inherited as a monogenic trait with early expression in these children. More than one genetic defect within the group is not excluded, but retrospective analyses of the 345 first-degree adult relatives of the affected parents indicated that most of the abnormal parents probably represented familial hypercholesterolemia, rather than combined hyperlipidemia, the other most generally recognized form of familial hyperbetalipoproteinemia.


The effect of cyclic AMP and dibutyryl cyclic AMP on the permeability characteristics of the renal tubule. The effect of adenosine-3',5'-cyclic monophosphate (cyclic AMP) and N(6),O(2)-dibutyryl adenosine-3',5'-cyclic monophosphate (dibutyryl cyclic AMP) on renal tubular permeability was studied by microinjection techniques in anesthetized diuretic rats. Radioactive inulin and mannitol were microinjected simultaneously into superficial proximal and distal convolutions and recovery of the isotopes was measured in the urine.During control conditions, mannitol and inulin recovery was essentially complete. However, during infusion of cyclic AMP or dibutyryl cyclic AMP, mannitol recovery was significantly less than control after early proximal and late proximal microinjections, averaging 79 and 85%, respectively. There was no loss of mannitol from the nephron after microinjection into distal convolutions. Inulin recovery was complete after all microinjections during cyclic AMP or dibutyryl cyclic AMP infusion. Simultaneous clearances of mannitol and inulin as well as peritubular capillary microinjections studies demonstrated bidirectional fluxes of mannitol across the proximal tubular epithelium during infusion of cyclic AMP or dibutyryl cyclic AMP. Intratubular pressures were not different during control and experimental periods. These studies demonstrate a change in the permeability characteristic of the proximal convoluted tubule during infusion of cyclic AMP and dibutyryl cyclic AMP. This change in permeability of the proximal tubule could account for the effects of cyclic AMP on proximal tubular transport processes.


Assay of total plasma apolipoprotein B concentration in human subjects. We have developed a double antibody radioimmunoassay (RIA) for human apolipoprotein B (ApoB). The assay measures not only the ApoB content of beta-lipoproteins (low density lipoproteins [LDL]) but also that contained in the other lipoproteins in plasma. Purified lymph and plasma chylomicrons and plasma very low density lipoproteins (VLDL) produced displacement curves in the assay system which paralleled those produced by pure LDL. Thus, the ApoB found in chylomicrons, VLDL, and LDL were immunologically identical. ApoB accounted for about 25 and 35%, respectively, of the total protein of chylomicrons and VLDL by RIA. VLDL and LDL preparations from normal and hyperlipoproteinemic subjects also produced parallel displacement curves, suggesting that the ApoB of normal and hyperlipoproteinemic subjects were immunologically identical. High density lipoproteins and abetalipoproteinemic plasma displaced no counts, nor did the sera of several animal species produce any useful displacement curves in this system. The fasting total plasma ApoB concentration of normal subjects was 83+/-16 mg/dl (mean+/-SD). ApoB levels were high in Type II (162+/-16), and less so in Type IV (112+/-24) and Type V (105+/-17).When plasma ApoB concentration in Type IV patients was graphed against plasma glycerides, two subpopulations, which may represent different genetic or biochemical subgroups, were apparent.ApoB concentration in individuals on constant diet and drug regimen was stable over weeks to months. Greater than 90% of ApoB of normal and Type II subjects was in the d > 1.006 plasma fraction. By contrast, only 50-80% of ApoB was in the d > 1.006 fraction in Types IV and V. Thus, hypertriglyceridemia was associated primarily with a redistribution of ApoB to the lighter density fractions; by contrast, in hypercholesterolemia absolute ApoB concentration was markedly increased.


Epstein-barr virus-negative human malignant T-cell lines. Two lymphoblastoid lines, CCRF-CEM and HSB-2, with properties of malignant cells, derived from children with leukemia secondary to lymphosarcoma, have T-cell properties and lack Epstein-Barr virus antigens.


Concordant segregation of the expression of SV40 T antigen and human chromosome 7 in mouse-human hybrid subclones. Subcloning of Simian virus 40 (SV40) T antigen-positive mouse-human hybrids, derived from the fusion of mouse cells deficient in thymidine kinase with SV40-transformed Lesch Nyhan fibroblasts, resulted in their segregation into T antigen-positive and negative subclones. Positive correlation between the presence of human chromosome 7 and the expression of SV40 T antigen was established in the subclones examined. These results negate the possibility of a transfer of the SV40 genome to a mouse chromosome.


Aldosterone: current concepts.

Aldosterone: current concepts.


Wilm's tumor: thirty-year review of cases in Buffalo.


Increased production of cyclic AMP in mechanically stressed alveolar bone in cats.


Timing of interceptive orthodontic measures based on stages of maturation.


Hereditary deafness in the white cat.

Hereditary deafness in the white cat.


Immunoprecipitation is inappropriate for the isolation of radiochemically pure albumin from tissues.


A method for isolating respiring mitochondria from epiphyseal cartilage.


A method for separating DNA fragments by electrophoresis in polyacrylamide concentration gradient slab gels.


Determination of 2,4-dihydroxy-1,4(2h)-benzoxazin-3-one glucosides in corn (Zea mays L.).


Rapid sampling of yeast cells and automated assays of adenylate, citrate, pyruvate and glucose-6-phosphate pools.


A high precision fluorometer for biochemical measurements.


Assay for the enzymic hydrolysis of penicillin and acylhydroxamates.


Adsorption chromatography of cyclic nucleotides on silica gel and alumina thin-layer sheets.


Microanalytical determination of the activities of phospholipases A, C, and D and of their mixtures.


Enzymatic synthesis of 32P-labeled coenzyme A.


Percentage recovery of free fatty acids from bovine muscle lipids by nonchromatographic techniques.


Separation of proteins by high-speed pressure liquid chromatography.


Antigenic structure of murine mammary tumour viruses.


The lack of effect of mouse epidermal growth factor (EGF) and its anti-serum on human tissues.


Carcinogenic effect of N-nitrosoamines related to butyl(4-hydroxybutyl)nitrosoamine in ACI-N rats, with special reference to induction of urinary bladder tumors.


Spin concentration per living cell of normal liver, regenerating liver, and hepatomas.


The evolution of a state hospital into a human-services center.


The impact of a mental health center on admissions to a state hospital system.


On the mechanism of thymine formation from thymine glycol by gamma-irradiation in aqueous system.


Ultrastructure of inclusion bodies in type II cells of lung, human and sub-simian.


The velocity of lymph flow in the canine thoracic duct. 1. The velocity and pattern of movement of lymph in the thoracic duct of anaesthetized and conscious dogs has been studied by observing the movement of droplets of ultrafluid lipiodol in the duct.2. The velocity of flow when anaesthetized varied from 0.1-2.0 cm/sec, to 5.0 cm/sec when conscious.3. The pattern of flow was affected by respiration and the cardiac cycle. Most movement occurred at the end of inspiration.4. The duct of five autopsy preparations was perfused with saline to assess the volume and velocity of flow produced by the level of pressure gradients previously observed in the duct. These studies show that the small gradients (2-5 mmHg) observed during life are more than sufficient to produce the normal volume and velocity of flow measured. The mean resistance of the duct was 0.5 mmHg/ml. min.


Analysis of the role of cyclic adenosine 3',5'-monophosphate in catecholamine release. 1. Cyclic adenosine 3',5'-monophosphate (cyclic AMP) levels and catecholamine release were measured in cat adrenal glands perfused in situ with Locke solution.2. Cyclic AMP was present in the medulla in an amount which represented approximately one fifth of that present in the cortex.3. Perfusion with acetylcholine (ACh) or nicotine increased cyclic AMP both in the intact adrenal and in the perfusate. The time course of the changes in tissue cyclic AMP during stimulation was out of phase with the time course of catecholamine release. Maximal increases in cyclic AMP were not manifest until after 8 min of exposure to the secretogogue, whereas maximal rates of secretion occurred during the first minute.4. Theophylline (0.5 mM) increased basal and stimulated adrenal cyclic AMP levels, but did not potentiate the secretory response to ACh or nicotine.5. Perfusion with cyclic AMP or its dibutyryl derivative (0.2-4 mM) failed to effect a consistent or significant increase in the rate of catecholamine release and was unable to potentiate the secretory response to a submaximal concentration of ACh or calcium.6. The results suggest that, unlike calcium, cyclic AMP is not a direct mediator of medullary secretion.


Plasma renin and angiotensin II in conscious pregnant ewes and their lambs.


Negative feed-back regulation of noradrenaline release by nerve stimulation in the perfused cat's spleen: differences in potency of phenoxybenzamine in blocking the pre- and post-synaptic adrenergic receptors. 1. The effects of low concentrations of phenoxybenzamine (8.8 x 10(-10) to 2.9 x 10(-7)M) on responses and on noradrenaline overflow elicited by nerve stimulation were studied in the perfused cat's spleen.2. In the presence of 8.8 x 10(-10)M or 2.9 x 10(-9)M phenoxybenzamine there was a significant reduction in responses to nerve stimulation while the overflow of the transmitter did not increase at the two frequencies of stimulation employed: 5 and 30 Hz.3. In the presence of 2.9 x 10(-8)M or 2.9 x 10(-7)M phenoxybenzamine the responses to nerve stimulation were practically abolished and a significant increase in transmitter overflow was obtained at both frequencies of stimulation. The drug was more effective in increasing transmitter overflow at 5 Hz when compared with 30 Hz.4. The higher effectiveness of phenoxybenzamine in blocking the post-synaptic alpha-receptor when compared with the blockade of the presynaptic alpha-receptor that regulates transmitter release is compatible with the view that these two receptors are not identical.5. A second alternative for the difference in effectiveness of phenoxybenzamine is that both types of alpha receptors are identical, but the spare receptor capacity for the presynaptic adrenergic receptors is higher than that of the post-synaptic receptors.


Gamma-aminobutyric acid and inhibition in the septal nuclei of the rat. 1. The electrophoretic application of gamma-aminobutyrate (GABA) and glycine to septal neurones inhibited their discharge, but the currents required to cause equivalent degrees of inhibition were always smaller for GABA than for glycine.2. The actions of GABA but not those of glycine were antagonized by bicuculline and not by strychnine and vice versa.3. The recurrent collateral inhibition of lateral septal neurones and the direct inhibition of medial cells evoked by stimulation of the fimbria were blocked by bicuculline but not by strychnine.4. The bursting patterns of discharge of laterally situated medial septal neurones was disrupted by bicuculline, but required a longer application of the drug than was needed to block the direct fimbrial inhibition. It is suggested that this disruption is due to diffusion of the drug to affect the inhibitory interneurones of the lateral septum.5. It is concluded that the inhibitory processes in the septum involve GABA as the synaptic transmitter.


Cell fusion induced by pederine.

Cell fusion induced by pederine.


Repeated partial endoscopic resections as treatment for two patients with inoperable tracheal tumours.


The preparation of single cell suspensions from liver and their use for the study of protein synthesis.


Prophylaxis of endemic goitre.

Prophylaxis of endemic goitre.


Five years follow-up in the treatment of endemic goiter by iodized oil.


Biochemical studies on endemic goiter glands before and one year after an injection of iodized oil.


Factors which may alter the epidemiology of endemic goiter.


Gonadotropins and ovarian function.

Gonadotropins and ovarian function. The current knowledge of the influence of gonadotropins on ovarian function is reviewed based primarily on human data but supplemented with references to animal studies.  2 aspects of ovarian function are under the influence of gonadotropins--follicular growth and maturation and steroid synthesis and secretion.  Follicular stimulatin hormone (FSH) promotes the growth of more advanced follicles.  Recent evidence suggests that estrogens increase the capacity of the ovary to bind FSH.  There is evidence that estrogen is produced in the theca interna of the maturing follicle.  It seems likely that the ultrastructural, enzymatic, and secretory changes seen in the thecae result from stimulation by low tonic levels of plasma luteinizing hormone (LH) in combination with FSH.  At ovulation the surge of gonadotropins is triggered off by the ri sing estrogen output.  Graafian follicles respond to LH in vitro with increased synthesis of progesterone and lactic acid.  The capacity to lutenize spontaneously develops before ovulation and perhaps the oocyte inhibits this function.  The development of the human corpus luteum is summariezed and the pattern of progesterone production described.  LH and human chorionic gondtropin acutely stimulate steroid synthesis by binding to specific sites on luteal cell menbranes to increase the synthesis of cyclic AMP.  The corpus luteum of early pregnancy differs morphologically and functionally from that of the cycle, increasing progesterone production which remains high until the 11th or 12th week of pregnancy.  The cortical stroma of the human ovary is steroidogenic i n character but producing androstenedion.  The stroma's capacity for synthesis varies with the cycle stage.  Each cell type synthesizes its characteristic spectum of steroid products.  How appropriate enzymes become activated in the cell and thus produce these steroids is as yet unknown.  Gonadotropins only initiate this differentiation and subsequently enhance the activity of transformed cells.


Direct effects of alcohol on in vivo and in vitro exocrine pancreatic secretion and metabolism.


Clinical implications of monoclonal gammopathy in chronic liver disease.


Bordetella pertussis serotypes in a whooping cough outbreak.


Cytomegalovirus and renal vein thrombosis in a newborn infant.


Disseminated herpes with hepatoadrenal necrosis in an adult.


Virus-induced diabetes mellitus. II. Relationship between beta cell damage and hyperglycemia in mice infected with encephalomyocarditis virus. Infection of DBA 2 male mice with the M variant of encephalomyocarditis virus resulted in a diabetes-like syndrome. Histologic examination of the pancreas revealed damage to the beta cells with little involvement of the acinar cells. The severity of the hyperglycemia correlated closely with the degree of beta cell damage. By immunofluorescence, viral antigens could be detected in the beta cells during the first 10 days of the infection. In contrast to the response found in male DBA 2 mice, infection of DBA 2 female mice and male mice of several other strains resulted in little if any elevation of blood glucose concentration. Histologic examination of the pancreas of these animals revealed only minimal damage to the beta cells. It is concluded that differences in the severity of the hyperglycemia between DBA 2 males and females and among the different strains of male mice tested are directly related to the degree of beta cell damage produced by the viral infection.


Metabolic defects of primary hyperuricemia and gout.


Neurotropic virus-host relationship alterations due to variation in viral genome as studied by electron microscopy. A series of experiments has been described in which litters of suckling rats were inoculated either with wild-type reovirus type III or one of two of its temperature-sensitive (ts) mutants. While the wild-type virus produced an acute, fatal syndrome, the ts mutants were substantially less neurovirulent. Of the ts mutant-inoculated animals, a large percentage of the surviving (chronic) animals given ts mutant B showed an unobstructive hydrocephalus ex vacuo whereas chronic ts mutant C animals showed no visible nervous system disease. The ts mutants persisted within the central nervous system (CNS) for 6 to 8 weeks, after which they could not be detected either virologically, immunologically or morphologically. In another set of experiments, organized CNS explants were studied following infection with either measles virus or the neuroadapted Mantooth strain of SSPE virus, a variant of measles. Wild measles (Edmonston strain) exerted an acute destructive effect, but SSPE virus had a tendency to enter into coexistence with the tissue without destroying its organotypic nature. These relationships are somewhat reminiscent of the neuropathologic conditions caused by these two viruses in man. Since the reovirus type III ts mutants possess both genetic and morphologic defects and in many instances cause CNS conditions different from that induced by the wild-type virus, it has been proposed that a comparable situation may exist after measles and SSPE virus infection. SSPE virions of the strain studied were found to be defective in certain viral components which may have contributed to the lower neurovirulence and its entering into a chronic relationship with the CNS, in contrast to the acute destructive nature of measles infection. The findings are discussed in terms of relevance to other chronic CNS diseases, particularly multiple sclerosis, in which the possiblity exists that a mutant virus is operative.


Selection and characterization of acriflavine-induced mutants of Candida albicans.


Initial steps in the degradation of n-alkane-1-sulphonates by Pseudomonas.


Pathology of colonic polyps as it relates to surgical management.


Subcellular fractionation of rat cerebellum: an electron microscopic and biochemical investigation. II. Resolution of morphologically characterised fractions.


Pathways of glycollate metabolism in the blue-green alga Anabaena cylindrica.


The effect of high doses of synthetic ACTH on rat brain.


A comparison of the interlaboratory reproducibility of the Hansen radioimmunoassay for carcinoembryonic antigen.


Corticosterone binding to hippocampus: immediate and delayed influences of the absence of adrenal secretion.


Action of angiotensin and analogues on the heart.


Androgen-induced enhancement of vascular reactivity.


Role of the N-terminal amino acid for the biological activities of angiotensin and inhibitory analogues.


Constancy and linearity of the metabolic clearance of adrenocorticotropin in dogs.


The diagnosis of malignant neoplasms in infants and children.


Catecholamine measurements in pheochromocytoma and neuroblastoma.


The effect of replacement of potassium by cesium ions on neuromuscular blockade of the rat phrenic nerve--diaphragm preparation in vitro.


The insulin-glucose ration in children with islet cell tumors of the pancreas.


Renin activity by radioimmunoassay: the plasma incubation step.


Germ cell tumors of the ovary: the role of pathology in diagnosis and management.


Gestational trophoblastic neoplasia.

Gestational trophoblastic neoplasia.


Editorial: The compensated polarized light microscope in clinical rheumatology.


Proceedings: Lymphocyte tubuloreticular structures--disease activity correlation and sequential studies in systemic lupus erythematosus.


IV. Joint erosion in rheumatoid arthritis.


Biochemistry of iron.

Biochemistry of iron.


Intracellular recording of spontaneous local responses in the chronic epileptogenic focus of a rhesus monkey.


Batrachotoxin, an extremely active cardio- and neurotoxin from the Colombian arrow poison frog Phyllobates aurotaenia.


Editorial: Status report: feline leukemia.


Preparation and use of soluble ferritin-antiferritin complexes as a specific marker for immunoelectron microscopy.


Enzymatic digestion of the first component of human complement (C1q).


Abnormalities in the mixed leukocyte reaction during infectious mononucleosis.


Wilms' tumor in the horseshoe kidney.

Wilms' tumor in the horseshoe kidney.


Physical biochemistry of calcification.

Physical biochemistry of calcification.


3H-actinomycin D binding to DNA localized in ultrathin sections of plastic-embedded biological materials.


Effect of mitotic inhibitors on C-type virion production.


Comparison of red cell angiotensinase activity in man and animals.


Staining methods of Australia antigen in paraffin section--detection of cytoplasmic inclusion bodies.


Endothelial injury and platelet aggregation assoicated with acute lipid mobilization.


Toxic metabolites released from rat hepatoma cells in culture. 2. Biological and chemical analyses of toxic metabolites.


Experimental hydrocephalus: surface alterations of the lateral ventricle. Scanning electron microscopic studies.


Oncorna virus disease. The syndrome of hemolytic anemia and lymph node cystic disease.


Effect of prophylactic measures on the microbial flora of patients in protected environment units.


Proceedings: New and simple polarized microscopy.


Studies of resonance energy migration in a heterogeneous pigment complex. I. Heterogeneity as a factor accelerating the localization of electron excitation in traps.


Conformational changes in spin-labeled antibodies and antigens during specific complex formation.


Letter: Adrenal responsiveness in chronic hemodialysis.


Letter: Neuropathy and methyl N-butyl ketone.


Letter: Mechanisms of lithium action.

Letter: Mechanisms of lithium action.


Leptomeningeal changes following lumbar myelography with water-soluble contrast media (meglumine iothalamate and methiodal sodium).


A landmark for the choroidal arteries of the fourth ventricle--branches of the posterior inferior cerebellar artery.


Studies of chicken sarcoma (strain 13). A review.


Interdependable effects of magnesium ion and ethylene diamine tetracetate (EDTA) on chicken liver D-fructose-1,6-diphosphate, 1-phosphohydrolase.


Effect of various nucleoside triphosphates on the catalytic activity of chicken liver fructose diphosphatases.


Haptoglobin levels in the plasma of newborn infants with special reference to infections.


Common bile duct obstruction by granular cell tumor (schwannoma).


Hepatoma, erythrocytosis and increased serum erythropoietin developing in long-standing hemochromatosis.


The history of infectious mononucleosis.


Phosphatase activity of anaerobic organisms. Anaerobic organisms were tested for phosphatase activity in different pH ranges. Several groups of organisms displayed characteristic patterns. Bacteroides fragilis, B. melaninogenicus, and B. ruminicola produced phosphatase with strongest activity at pH 8.6. Fusobacterium mortiferum was the only species of this genus to show strong hydrolysis. The enzyme was active in both acid and alkaline ranges. The activity of gram-positive organisms was variable, the most active groups being Clostridium perfringens, Peptostreptococcus intermedius, P. micros, and Peptococcus constellatus. The incorporation of phosphatase activity into the identification scheme of anaerobes seems feasible. There was a correlation of hydrolysis with several important pathogens.


Relationship of cellular fatty acid composition to survival of Lactobacillus bulgaricus in liquid nitrogen. Concentrated cultures of Lactobacillus bulgaricus were prepared by resuspending cells grown in semisynthetic media in sterile 10% non-fat milk solids. The concentrated cultures were frozen in liquid nitrogen for 24 h. The cell suspensions exhibited decreased viability after storage, and the amount of death varied among the different strains tested. Storage stability of all strains examined was improved by supplementing the growth medium with sodium oleate. Radioisotopes were used to study the fate of sodium oleate with L. bulgaricus NCS1. [1-(14)C]sodium oleate was incorporated solely into the lipid portion of the cells, including both neutral and polar lipids. The fatty acid composition of L. bulgaricus NCS1, NCS2, NCS3, and NCS4 grown with and without sodium oleate was studied. The major fatty acids of strains NCS1, NCS2, and NCS3 grown without sodium oleate were dodecanoic, tetradecanoic, hexadecanoic, hexadecenoic, and octadecenoic acids. In addition to these, strain NCS4 contained C(19) cyclopropane fatty acid. The major fatty acids of all strains grown with sodium oleate were tetradecanoic, hexadecanoic, hexadecenoic, octadecenoic, and C(19) cyclopropane fatty acids. All strains grown in broth containing sodium oleate contained larger amounts of octadecenoic and C(19) cyclopropane fatty acid, and less saturated fatty acids than when grown without sodium oleate. Statistical analyses indicated that C(19) cyclopropane fatty acid was most closely related to stability of the lactobacilli in liquid nitrogen. A negative regression line that was significant at P < 0.001 was obtained when the cellular content of this fatty acid was plotted against death.


Evaluation of media, time and temperature of incubation, and method of enumeration of several strains fo Clostridium perfringens spores. Two basal media, containing the ingredients found in common in both SPS (BBL) and TSN (BBL) media and in the previously described media of Schaedler et al. (1965) and Starr et al (1971), but minus antibiotics, were selected as the most suitable for the enumeration of Clostridium perfringens spores in a model system. These media were also used to study the influence of the presence of glucose, xylose, or ribose in various concentrations (0, 0.01, 0.1, and 1.0%) on colony morphology and spore recovery. As the sugar concentration in the basal agar medium increased, the colonies of all the test organisms also increased in size, and more of the black colonies became white in color. At the 1.0% sugar level, glucose permitted only white colony development, whereas the pentoses were completely inhibitory. Both pour plates and most-probable-number tubes were inoculated with the spores of several strains of C. perfringens and incubated at 20, 30, 37, and 45 C for 24, 48, and 72 h. Statistical analyses of the enumeration data indicated, at the 99% confidence level, that a Trypticase(BBL)-yeast extract-glucose-sulfite-iron agar gave maximal population estimates at 37 C in 72 h.


Requirement for and sensitivity to lysozyme by Clostridium perfringens spores heated at ultrahigh temperatures. The requirement of ultrahigh temperature (UHT)-treated Clostridium perfringens spores for lysozyme and the sensitivity of heated and unheated spores to lysozyme were studied. The UHT-treated spores requiring lysozyme for germination and colony formation originated from only a small portion of the non-UHT-treated spore population. This raised a question of whether the requirement for lysozyme was natural to the spores or was induced by the UHT treatments. However, these spores did not require lysozyme for germination before UHT treatment, which confirmed that the requirement for lysozyme had been induced by the UHT treatment. Only 1 to 2% of the spores were naturally sensitive to lysozyme; therefore, the mere addition of lysozyme to the plating medium did not permit the enumeration of all survivors. Treatment of UHT-treated spores with ethylenediaminetetraacetate (EDTA) sensitized the spores to lysozyme and increased by 10- to 100-fold the number of survivors that were detected on a medium containing lysozyme. Under the heating conditions used, spores that were naturally sensitive to lysozyme and spores that required EDTA treatment were equally heat resistant.


Ultrastructure of hepatoma cells detected in peritoneal fluid.


Specificity and sensitivity of the streptozyme test for the detection of streptococcal antibodies. A comparison between the results of the streptozyme hemagglutination test and serological titers for anti-streptolysin O (ASO), anti-hyaluronidase (AH), anti-deoxyribonuclease B (ADN-B), and anti-nicotinamide adenine dinucleotidase (ANAD) was made in two groups of human sera. In one group, serological titers for all the four antibodies were lower than the threshold of sensitization reported by the producing firm. In the second group, the titer of at least one of the four antibodies was equal to or higher than the threshold. False-positive and false-negative reactions occur with those sera when one or more antibody titer is at or near the threshold of the test as described by the manufacturer. The test was positive for all sera where either the ASO was greater than 166 or the ANAD was greater than 270, and for 98% of the sera with ADN-B greater than 360. It is, therefore, concluded that the streptozyme test can be used as an adjunct to the clinical diagnosis of streptococcal infections and their nonsuppurative sequelae. It is less useful to assess the levels of antibodies in sera from general population surveys. For such sera, the relative specificity and sensitivity of the test might yield misleading results. Until more experience is gained with the test, caution should be used in its application to infant and older adult age groups, where significant streptococcal antibody titers are frequently near the threshold of the test.


Developmental changes in vascular reactivity in neonatal skeletal muscle.


Aggression, schizophrenic reactions and epilepsy: some transcultural correlations.


Chronic venous insufficiency. Discussion and case report of statis ulcer treatment.


Synaptic interactions of reticulospinal neurons and nerve cells in the spinal cord of the sea lamprey.


The effect of collagenase-inhibitor complexes on collagenolytic activity of normal and inflamed gingival tissue.


Synaptic interactions of identified nerve cells in the spinal cord of the sea lamprey.


A light and electron microscopic study of an atypical calcifying odontogenic tumor containing "amyloid".


Electron microscopic studies of organ cultures of tooth germs infected with polyoma virus.


A serological survey for selected viral infections of Rocky Mountain bighorn sheep.


Editorial: Myelography.

Editorial: Myelography.


Bluetongue virus and white-tailed deer in an enzootic area of Texas.


Differentiation of blood islands in chick blastoderms in association with Morris hepatoma grafts and its relationship to specific chromosomal changes in the hepatomas.


Angiotensin II- and angiotensin 3-induced aldosterone release vivo in the rat. The potential role of angiotensin II and its heptapeptide metabolite, des-aspartyl-angiotensin II, was studied in the conscious unanesthetized rat. Aldosterone release was induced by both peptides at physiologic doses (0.72 nanogram per minute). [I-Sarcosyl-8-alanyl]-angiotensin II (P-113 inhibited angiotensin II more effectively than des-aspartyl-angiotensin II (101 percent as compared to 82 percent). These results indicate that angiotensin controls aldosterone release in the rat and that des-aspartyl-angiotensin II (that is, angiotensin III) may be important in this sequence.


Characterization of "heavy" and "light" SV40-like particles from a patient with PML.


Host range studies on xenotropic type C viruses in somatic cell hybrids.


Poliovirus proteins associated with ribosomal structures in infected cells.


Integration of SV40 DNA and induction of cellular DNA synthesis after a ts SV40 infection.


Fractionation of the double-stranded RNA of maize rough dwarf virus subviral particles.


Adsorption chromatography of polioviruses on porous glass.


Dependence of interferon induction on cell membrane integrity.


Skeletal maturation of the hand and wrist, a longitudinal study covering two years among children of Finnish Lapps.


A comparison between histochemical and biochemical data on the optic lobe of the chicken embryo after unilateral removal of the eye.


"Caveolated cells" characterized by deep surface invaginations and abundant filaments in mouse gastro-intestinal epithelia.


Vinblastine-induced aggregates of smooth endoplasmic reticulum in proximal tubular cells of rat kidney.


An ultrastructural comparison of the uptake and transport of horseradish peroxidase by the rat visceral yolk-sac placenta during mid- and late gestation.


Ultrastructure of the mouse superior cervical ganglion, with particular reference to the pre- and postganglionic elements covering the soma of its principal neurons.


Purification and characterization of a cellular fibrinolytic factor associated with oncogenic transformation: the plasminogen activator from SV-40-transformed hamster cells.


Effects of glucagon on activities of hepatic enzymes in sheep.


Pacemaker activity during aggregation in Dictyostelium discoideum.


Nucleoside triphosphate hydrolase activities of avian vitelline membrane and oviductal lumen.


Nicotinamide adenine dinucleotide levels in cells of developing chick limbs: possible control of muscle and cartilage development.


Red to white fiber ratios as an index of double muscling in beef cattle.


Simultaneous measurements in awake rats of drug-induced changes in carotid and tail-cuff systolic pressures.


Glioblastoma multiforme with remote extracranial metastases.


In vitro degradation and stabilization of the glucocorticoid binding component from mouse fibroblasts.


Steroid-receptor in Pseudomonas testosteroni released by osmotic shock.


Induction of steroid-binding activity in Pseudomonas testosteroni.


The influence of synthetic anti-estrogens on the binding of tritiated estradiol-17beta by cytosols of human uterus and human breast carcinoma.


Otoneurology.

Otoneurology.


Clinical neurology.

Clinical neurology.


Neuroophthalmology.

Neuroophthalmology.


Genral neurophysiology (biochemical aspects).


Peripheral nerve surgery.

Peripheral nerve surgery.


Neuroendocrine relationships.

Neuroendocrine relationships.


Neuropathology.

Neuropathology.


Neutrophil leukocyte collagenase, elastase and serum protease inhibitors in human gingival crevicles.


Two-dimensional polyacrylamide gel electrophoresis separation of low molecular weight nuclear RNA.


Inhibition of adenosine 3',5'-monophosphate accumulation in anterior pituitary gland in vitro by growth hormone-release inhibiting hormone.


Competitive inhibition of the transport of nucleosides, hypoxanthine, choline and deoxyglucose by theophylline, papaverine and prostaglandins.


Inhibition of deoxyribonuclease in an extract of rat liver nuclei by poly(ADP-ribose).


"In vitro" binding of cellular, alpha-amanitin sensitive, RNA polymerase to infectious, mengovirus-induced double-stranded RNA.


Corticosterone suppression of ACTH secretion: actinomycin D sensitive and insensitive components of the response.


Evidence from the control of pyrimidine biosynthesis in tissue minces by purines.


Studies of vanadium toxicity in the rat. Lack of correlation with molybdenum utilization.


Interactions of parathyroid hormone and plasma membranes from rat kidney.


Lymphocyte and polymorphonuclear enzymes in stress. V. Changes associated with a viral infection: Herpes simplex.


Elevation of hepatic phenylalanine: pyruvate aminotransferase by dibutyryl cyclic AMP in rats.


A brief review of pulmonary clearance of bacterial aerosols emphasizing aspects of particular relevance to veterinary medicine.


Combination therapy: some concepts and results.


Rationale of combined modality therapy for cancer.


In vitro-in vivo studies on the susceptibility of the solid Yoshida sarcoma to drugs and hyperthermia (42 degrees).


5'-Nucleotide phosphodiesterase activity in rat hepatoma.


Alpha-(N)-heterocyclic carboxaldehyde thiosemicarbazone inhibitors of ribonucleoside diphosphate reductase.


Virus oncogenesis and tumor immunogenicity in the mouse mammary tumor system.


In vitro determination of thymidine-3H labeling index in human solid tumors.


Beta-aminoisobutyric acid, a new probe for the metabolism of DNA and RNA in normal and tumorous tissue.


Concanavalin A-induced agglutination and tumorigenicity in virally and spontaneously transformed cells derived from BALB-c mice.


Relationship of rat alpha 1-fetoprotein to growth rate and chromosome composition of Morris hepatomas.


Effects of carcinogens and other agents on histone methylation by a histone arginine methyltransferase purified from rat liver cytoplasm.


Effects of carcinogens and other agents on histone methylation in rat liver nuclei by endogenous histone lysine methyltransferase.


Isozymes of pyruvate kinase in liver and hepatomas of the rat.


Isolation of a transplantable cell line induced by the MC29 avian leukosis virus.


Metabolic regulation and adenyl cyclase activity of adrenocortical carcinoma cultured cells.


Separation and characterization of the soluble and insoluble components of insoluble laminaran.


Chemical analysis of exocellular, acid polysaccharides from seven Rhizobium strains.


Improved lipid extraction of erythrocytes.


Quantitative immunoelectrophoresis of plasma proteins in acute viral hepatitis, extrahepatic biliary obstruction, primary biliary cirrhosis and idiopathic haemochromatosis.


Control of mitogen--induced lymphocyte activation.  I. The effect of cyclic AMP on DNA and RNA synthesis in PHA--stimulated cells.


Pyrophosphate stimulation of calcium uptake into cultured embryonic bones. Fine structure of matrix vesicles and their role in calcification.


Meeting report Twelfth Midwinter Conference of Immunologists. January 6-9, 1973.


Further studies on the localization of peroxidase in pig thyroid cells.


5Alpha-dihydrotestosterone "receptor" in the rat hypophysis.


Action of angiotensin on uptake, release and metabolism of 14C-noradrenaline by isolated rabbit hearts.


Training mental health workers to better meet patient needs.


Using college students as paraprofessionals.


Mechanism of adrenaline-induced lipolysis in adipose tissue.


Changes in adenosine 3',5'-monophosphate level and protein kinase activity by glucagon in rat liver nuclei.


Further investigations on the chemical structure of Forssman globoside obtained from caprine erythrocyte stroma.


Membrane-associated adenosine 3',5'-monophosphate-dependent protein kinase and its substrates in human erythrocytes.


Studies on low temperature spectra of respiratory pigments. II. Spectra of cytochromes in respiratory systems between liquid helium and room temperatures.


A spin-label study on calcium-induced conformational changes of troponin components.


The formate hydrogenlyase activity of cytochrome c552-deficient mutants of Escherichia coli K12.


Resistance to skin tumorigenesis by 3-methylcholanthrene in mice susceptible to leukemia.


Feline malignant mammary tumors. II. Immunologic and electron microscopic investigations into a possible viral etiology.


Tumor grading by implantation in embryos. I. Grading of minimum-deviation hepatomas.


Membrane antigen of bovine papilloma virus-induced fibroma cells.


Restriction of herpes simplex virus type 1 replication in oncornavirus-transformed cells.


Type-C RNA virus isolated from SJL-J mice.


Influence of catalase and superoxide dismutase on side oxidations involving singlet oxygen.


Transplacental effects of 1-ethyl-1-nitrosourea in inbred strains of mice. IV. Rapid tumor induction in strain crosses.


Properties of the common antigen associated with Marek's disease herpesvirus and turkey herpesvirus infections.


Effect of lifetime administration of 2-hydroxyethylhydrazine on tumorigenesis in hamsters and mice.


Spontaneous tumors and C-type virus in polyoma-infected aging wild house mice.


Transplantation studies on polyoma virus-infected cultures of mouse tooth germs.


Additional evidence of type-C particles in human placentas.


Effect of dibutyryl cyclic adenosine 3',5'-monophosphate on in vivo growth of Walker 256 carcinoma: isolation of responsive and unresponsive cell populations.


An attenuated mouse leukemia virus. I. Origin and immunization.


Antibodies to Epstein-Barr virus-associated antigens in relatives of cancer patients.


B-cell characteristics of human peripheral and cord blood lymphocytes transformed by Epstein-Barr virus.


Mammary tumor virus in strain GR mice in relation to age and tissue type.


Evaluation of an in vitro assay system for carcinogens based on prior infection of rodent cells with nontransforming RNA tumor virus.


Diversity of envelope antigens on murine type-C RNA viruses.


Expression of genes for intracisternal A-particle antigen in somatic cell hybrids.


C-type virus particles in a reptilian tumor.


Differential effect of ionizing radiation on mammary tumor virus activity associated with blood cells and mammary tumor cells.


Hemic cell-associated mammary tumor virus activity in BALB-cfC3H mice.


Stimulated growth of syngeneic tumors at the site of an ongoing delayed-hypersensitivity reaction to tuberculin in BALB-c mice.


Transmission of mammary tumor virus by the GR mouse strain. I. Role of the virus in the production of lesions.


Morphology of a "noninfectious" sarcoma virus.


Procedure for activating Epstein-Barr virus early antigen in nonproducer cells by 5-iododeoxyuridine.


Cellular immunity in mice with simian virus 40-induced mKSA tumors: comparison of three assays of tumor immunity.


Glandular epithelial cells from mice: a method for selective cultivation.


Neurotropic effect of 7,12-dimethylbenz(a)anthracene in transplacental carcinogenesis.


Ultrastructural comparison of placental virus with several type-c- oncogenic viruses.


Biogenesis of cocarboxylase in Escherichia coli: regulatory properties of thiamine monophosphate kinase.


Lipids of cultured hepatoma cells. 3. Triglyceride and phosphoglyceride biosynthesis in minimal deviation hepatoma 7288C.


Diseases in feeder calves.

Diseases in feeder calves.


On the levels of alkyl and alk-1-enyl glycerolipids in normal and neoplastic tissues: a method of quantification.


The functional significance of scolex retraction and subsequent cyst formation in the cysticercoid larva of Hymenolepis microstoma.


The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function.


An in vitro investigation of dynamic processes occurring in the schistosome tegument, using compounds known to disrupt secretory processes.


Oxaloacetate metabolic crossroads in liver. Enzyme compartmentation and regulation of gluconeogenesis.


Neonatal islet cell adenoma: case report and literature review.


Liver-spleen scanning in pediatrics.

Liver-spleen scanning in pediatrics.


Studies on hypoglycaemia in primary carcinoma of the liver. A case report and review of pathogenetic mechanisms.


Formation of receptors of glucocorticoid hormones in rat liver chromatin and cytoplasm in ontogenesis.


Action of corticosteroids and ACTH on the chromaffin cells of chick embryo's adrenal glands in organ culture.


Differentiation of water-conducting elements in plants.


The morphology of experimental tumours of the nervous system. II. Intracerebral tumours in the rat.


Oncocytes in the anterior lobe of the human pituitary gland. A light and electron microscopic study.


Heterogeneity and biochemical findings in manic-melancholic disorders.


Respiratory control in cytochrome oxidase.


Cyclic 3',5'-adenosine monophosphate stimulates trehalose degradation in baker's yeast.


A novel property of mitochondrial oxidative phosphorylation.


Demethylamination of N6-methyldeoxyadenosine by commercial spleen phosphodiesterase preparations.


Kinetics of reactions involving C-550 and cytochrome b559 in chloroplasts at low temperature. Evidence for two photoreactions.


A possible termination factor for transcription in Escherichia coli.


The reaction of the superoxide ion with iron (3) protoporphyrin IX dimethyl ester perchlorate: evidence for a stable dioxygen complex.


The effect of cyclic AMP and related compounds on the control of protein synthesis in reticulocyte lysates.


Cyclic adenosine 3':5'-monophosphate levels in normal and transformed cells of higher plants.


Deuterium and carbon-13 tracer studies of ethanol metabolism in the rat by 2H, 1H-decoupled 13C nuclear magnetic resonance.


The heme environment in ferric and ferrous cytochrome c oxidase.


On the specificity and pH dependence of ficin-catalyzed hydrolyses. Some comparisons with bromelain specificity.


Influence of globin structure on the state of the heme. 3. Changes in heme spectra accompanying allosteric transitions in methemoglobin and their implications for heme-heme interaction.


Prefrontal unit activity during delayed alternation in the monkey. I. Relation to direction of response.


Prefrontal unit activity during delayed alternation in the monkey. II. Relation to absolute versus relative direction of response.


Correlation between brain adenyl cyclase activity and spontaneous motor activity in rats after chronic reserpine treatment.


Early onset in inhibitory functions during synaptogenesis in fetal mouse brain cultures.


Molecular species and membrane forming properties of lecithins in normal liver and hepatoma.


Upper limit of autoregulation of cerebral blood flow in the baboon.


Stimulation of prostaglandin biosynthesis by adenine nucleotides. Profile of prostaglandin release by perfused organs.


Radioimmunoassay of angiotensin I, for estimation of plasma renin activity.


Depressed fatty acid and acetate oxidation and other metabolic defects in homogenates from hearts of hamsters with hereditary cardiomyopathy.


Evaluation of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides in the hexokinase method for determining glucose in serum.


Ontogenesis of the renin-angiotensin system in spontaneously hypertensive and normal Wistar rats.


Optic radiation activity during sleep and waking.


Binding of D-tubocurarine and alpha-bungarotoxin in normal and denervated mouse muscles.


Excitation of cerebral cortical neurons by various polypeptides.


Electron microscope studies of Fasciola hepatica. XI. Autophagy and parenchymal cell function.


Hormonal responses to intravenous and oral glucose tolerance testing in a patient with a gastrinoma and a gastrojejunostomy.


Route of protein loss during a model protein-losing gastropathy in dogs.


Saccadic discharges evoked by intracellular stimulation of extraocular motoneurons.


Primary afferent hyperpolarization and presynaptic facilitation of Ia afferent terminals induced by large cutaneous fibers.


Contribution of nonspecific thalamus to sensory evoked activity in cat postcruciate cortex.


Neural control of circulation in Aplysia. I. Motoneurons.


Neural control of circulation in Aplysia. II. Interneurons.


Furosemide--pharmacology and cellular mode of action.


The use of multiple systemic antibiotics in the treatment of patients with burns.


The carcinogenic effect of 2-oxo-propyl-propylnitrosamine in Sprague-Dawley rats.


Action of ascorbic--iron combination on the synthesis of blood cells and on the activity of phosphatase enzymes.


Megakaryocyte abnormalities in thrombotic thrombocytopenic purpura.


Experience with infusion and resection in cancer of the liver.


Energy coupling and extramitochondrial electron transport in slices of Morris hepatoma 3924 A.


Effect of polymyxin B on antibiotic-resistant Proteus mirabilis. The surface properties of polymyxin B-resistant Proteus mirabilis are markedly altered by the antibiotic. The effects include the development of susceptibility to surface-active agents such as deoxycholate or tris(hydroxymethyl)aminomethane and a marked increased osmotic fragility. However, cell wall impermeability to various agents such as erythromycin, actinomycin D, bacitracin, lysozyme, and triphenyltetrazolium chloride apparently remains intact. These results support the concept that the actions of the polymyxins on gram-negative bacteria are multiple; the action(s) of the antibiotic responsible for lethality in vitro, presumably at the level of the cytoplasmic membrane, may represent only a portion of the potential therapeutic effects of the antibiotic in vivo.


Locus of divalent cation inhibition of the bactericidal action of polymyxin B. The bactericidal effect of polymyxin B upon two Pseudomonas aeruginosa strains and two Escherichia coli strains was studied as a function of the concentration of the divalent cations Ca(2+) and Mg(2+). Lipid spherules in aqueous suspension (liposomes) were prepared from the bacterial phospholipids and were examined for the release of trapped glucose induced by polymyxin B under similar conditions of divalent cation concentration. In contrast to the bacteria, which were protected markedly from the bactericidal action of polymyxin B by Ca(2+) or Mg(2+) at concentrations of 2 x 10(-3)m or less, neither cation had any protective effect on bacterial liposomes at concentrations up to 2 x 10(-2)m. These observations suggest that divalent cations antagonize the bactericidal effect of polymyxin B indirectly through interaction with the cell wall rather than at the primary membrane locus of action. The interaction of the divalent cations with the cell wall prevents access of the antibiotic to the cytoplasmic membrane by mechanisms that remain unclear.


Standardized antimicrobial disc susceptibility testing of anaerobic bacteria: in vitro susceptibility of Clostridium perfringens to nine antibiotics. The in vitro susceptibility of 43 strains of Clostridium perfringens to nine antibiotics was determined by a standardized test for rapid-growing anaerobes. Good correlation was established between the agar dilution susceptibility and the disc diffusion susceptibility results. The inhibition zone diameters around the antibiotic discs, however, were generally much smaller than those of gram-negative anaerobes previously studied. All of the strains tested were susceptible in vitro to chloramphenicol, clindamycin, doxycycline, minocycline, penicillin, and vancomycin. Erythromycin showed poor in vitro activity against this organism, with only 7% of the strains susceptible, 72% intermediate in susceptibility, and 21% resistant. In tests of the 43 strains against lincomycin, 58% were susceptible, 32.5% were intermediate in susceptibility, and 9.5% were resistant. Against tetracycline, 37% of the strains were intermediate in susceptibility and the rest were susceptible.


Antiviral action of camptothecin.

Antiviral action of camptothecin. At a concentration of 10 mum, camptothecin inhibited vaccinia deoxyribonucleic acid (DNA) synthesis in HeLa cells. Inhibition of viral DNA synthesis was observed when the drug was added before infection or at 1 or 2 hr after infection. Inhibitory effects of camptothecin on vaccinia DNA synthesis could be reversed, even after exposure to the alkaloid for 2 hr. Viral DNA, isolated from vaccinia-infected, camptothecin-treated cells, displayed an altered sedimentation constant after alkaline sucrose density gradient centrifugation. Incorporation of uridine into vaccinia messenger ribonucleic acid was inhibited by camptothecin, but the activity of ribonucleic acid polymerase, as tested in isolated vaccinia cores, was not affected by the drug. Camptothecin had essentially no effect on replication of poliovirus in HeLa cells.


The Guillain-Barré syndrome. A clinical and electrodiagnostic study of 25 cases.


Pituitary tumours.

Pituitary tumours.


Autonomous breathing. Report of a case.

Autonomous breathing. Report of a case.


Effect of "medical" and surgical adrenalectomy on the ventral prostate and other accessory sex glands of the male rat.


Intermediary mechanisms in asthma. Some informal comments on the pathogenesis of the breathing disturbances.


A cytological and genetic study of oogenesis in Drosophila melanogaster.


Microtubules in the cortical region of the egg of Lymnaea during cortical segregation.


Protein synthesis and secretion in a myogenic cell line.


Changes in adenosine 3',5'-monophosphate induced by parathyroid hormone and theophylline in the developing rat renal cortex.


Ultrastructural studies of lizard (Anolis carolinensis) myogenesis in vitro.


Inhibition of ribosomal RNA synthesis in neurula cells by extracts from blastulae of Xenopus laevis.


Adhesive specificity in normal and transformed mouse fibroblasts.


Stimulation of ciliary activity by low levels of extracellular adenine nucleotides in the amphibian oviduct.


Preparation and characterization of chick erythrocyte nuclei from heterokaryons.


N6-substituted adenines induce cell elongation irrespective of the intracellular cyclic AMP levels.


The influence of serum proteins and pH on the effective amino acid concentration in tissue culture medium.


Suitability of silica sol gradients for purification of cell organelles.


Different cyclic changes in the surface membrane of normal and malignant transformed cells.


Electron microscope observations on the cell walls of some gram-positive bacteria.


Phagocytic activity in the retinal pigment epithelium of the frog Rana pipiens. II. Exclusion of Sarcina subflava.


Kupffer cell reactions in rat liver under various conditions as observed in the electron microscope.


Observations on the fine structure and peroxidase cytochemistry of normal rat liver Kupffer cells.


Peripheral nerve function in long-term therapy with diphenylhydantoin. A clinical and electrophysiologic correlation.


Paroxysmal signs and symptoms in multiple sclerosis.


Adverse interaction between steroid hormones and anticholinesterase drugs.


Morphologic changes associated with combined BCNU and radiation therapy in glioblastoma multiforme.


Effect of pentoxifylline on cerebral edema in cats.


Endogenously blocked high affinity estradiol receptors in the immature and mature rat uterus.


Effect of intravenous infusion of isethionate on rats.


Comparative studies of the viruses rescued from polyoma tumor cell line.


Effects of glucagon and dibutyryl cyclic AMP on the phosphorylase activity and gluconeogenesis in rat liver slices.


Estrogen receptors in bone. An evaluation of the uptake of estrogen into bone cells.


Evidence for the involvement of superoxide anion in dopamine-beta-hydroxylase system.


Typing of isolates of herpes simplex-virus by mixed agglutination.


Induction of tumors of the stomach and esophagus in inbred Chinese hamsters by oral diethylnitrosamine.


Pituitary-adrenal function in adult androgenized female rats.


Specific unresponsiveness to skin allografts in mice. 3. Synergistic effect of tissue extracts, Bordetella pertussis, and antilymphocytic serum.


A histochemical study on the interstitial cells of the rat renal medulla.


Liver biopsy.

Liver biopsy.


Hyperresponsiveness to lysine-vasopressin in Cushing's disease.


Plasma testosterone in male puberty. I. Physiology of plasma testosterone.


Biological activity of synthetic human corticotropin with revised amino acid sequence.


The uptake and metabolism of (4-14C)testosterone in human breast cancer growth in organ culture.


Growth retardation in the Lesch-Nyhan syndrome.


On the regulation of estrogen production by cortisol and ACTH in human pregnancy at term. Memorial Foundation award thesis.


Angiography in gastric tumours.

Angiography in gastric tumours.


Herpesvirus infections in pregnancy: a comparison of neutralizing antibody titers in mothers and their infants.


Sodium dodecylsulfate-dependent anomalies in gel electrophoresis: alterations in the banding patterns of foot-and-mouth disease virus polypeptides.


Activation of DNA primer for DNA polymerase as an ultrasensitive assay for a subgroup of enzymes with deoxyribonuclease activity.


A sensitive analytical method for the detection and quantitation of adenosine in biological samples.


An improved protein binding assay for cyclic AMP.


A fast and sensitive method for the analysis of modified nucleosides in tRNA.


The effect of two synthetic ACTH analogues on the metabolism of biogenic amines in the rat brain.


The assay of ATP by the luciferin-luciferase method. Interference by a bacterial phosphatase enzyme stable to perchlorate treatment.


Experimental disciform serous retinal detachment. I. Method of production and histology.


Experimental disciform serous retinal detachment. II. Autoradiography of photoreceptor protein metabolism.


Pseudomonas blepharoconjunctivitis: a complication of combination chemotherapy.


Studies on the radioiodination of very low density lipoprotein obtained from different mammalian species.


Long-term storage of erythrocytes for quantitative analyses of lipids.


Proceedings: A possible gene assignment to chromosome 21.


A review of methods and metabolic studies associated with the radioiodination of lipoproteins.


Proceedings: The human loci for phosphopyruvate hydratase and guanylate kinase are syntenic with the PGD-PGM1 linkage group in man-Chinese hamster somatic cell hybrids.


Proceedings: Assignment of the gene in man for mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6.


Proceedings: The genetics of the antiviral state in human cells.


Proceedings: Assignment of the second locus of adenylate kinase to chromosome 1p: preliminary data.


Proceedings: Evidence for synteny between the human loci for the HLA histocompatibility antigen, phosphoglucomutase3, malic enzyme (cytoplasmic), and indophenol oxidase B (tetrameric).


Proceedings: Regional assignments of seven enzyme loci on chromosme 1 of man.


Proceedings: The expression of hepatic functions in mouse hepatoma x human leukocyte hybrids.


Temperature-sensitive mutations in Drosophila melanogaster. XI. Male sterile mutants of the Y chromosome.


Studies of oogenesis in Urechis caupo. II. Accumulation during oogenesis of carbohydrate, RNA, microtubule protein, and soluble, mitochondrial, and lysosomal enzymes.


Sleep, sleep deprivation, photosensitivity and epilepsy.


5-Hydroxyindoleacetic acid and homovanillic acid in cerebrospinal fluid of patients with neurological diseases.


The deoxyribonucleoside transport systems of cultured Novikoff rat hepatoma cells.


Intracellular conversions of deoxyribonucleosides by Novikoff rat hepatoma cells and effects of hydroxyurea.


Effect of growth conditions on the activity of ornithine decarboxylase in cultured hepatoma cells. I. Effect of amino acid supply.


The effect of calcium concentration on ACTH stimulation of steroidogenesis in mouse adrenal tumor cells.


The nervous control of autotomy in Carcinus maenas.


Plasma membrane ectoglycosyltransferase activity of L1210 murine leukemic cells.


Angiofibroma: a treatment approach.

Angiofibroma: a treatment approach.


The medical and surgical treatment of nasal polyps.


Role of the spleen in membrane conditioning and hemolysis of spur cells in liver disease.


Treatment of hyperlipidemia.

Treatment of hyperlipidemia.


Letter: Hypertension and hydronephrosis.


Studies on the neuropharmacology of papaverine. 3. The inhibition of ganglionic transmission.


Thyroid thermogenesis.

Thyroid thermogenesis.


Comparative study of brain scintiphotography, cerebral angiography and pneumencephalography in detection of gliomas.


The vascular supply of eosinophilic and mixed pituitary adenomas in acromegalic patients.


Radioisotope serial brainscanning.

Radioisotope serial brainscanning.


Aspects of the venous drainage of the pons and the mesencephalon.


Type-specific tumor patterns in the cerebral radioangiography (CRAG): results in 195 patients.


Functional radioangiographic studies of cerebral vascular haemodynamics (preliminary report).


A case of chronic adhesive arachnoiditis after lumbar myelography with methiodal-sodium.


Diagnostic problems of cerebral metastases.


Cytomegalic inclusion diseases; a clinico-pathological correlation.


Renin-angiotensin activation in hyperacute renal xenograft rejection.


Erythrocyte association and interferon production by minute virus of mice.


Inhibitory effect of sodium cyclamate and sodium saccharin on tumor induction by 2-acetylaminofluorene in rats.


Activation of the renal erythropoietic factor by a cyclic AMP-dependent protein kinase.


Inhibition at the T-cells of the spinothalamic tract: a neurophysiological basis for electrically induced analgesia.


Histochemical enzyme changes in epidermis of manganese-deficient fetal mice.


Effects of adrenal steroids on brain function and behavior.


Effects of acute and chronic adminstration of narcotic analgesics on growth hormone and corticotrophin (ACTH) secretion in rats.


How do neurones secrete peptides? Exocytosis and its consequences, including "synaptic vesicle" formation, in the hypothalamo-neurohypophyseal system.


Effects of morphine and related drugs on the corticotrophin (ACTH)-stress reaction.


Behavioral aspects of melanocyte-stimulating hormone (MSH).


The influence of peptides derived from corticotrophin (ACTH) on performance. Structure activity studies.


Antagonism between morphine and the polypeptides ACTH, ACTH1-24 and beta-MSH in the nervous system.


Steroid feedback mechanisms in pituitary-adrenal function.


Effect of short-term upright posture on plasma angiotensin II in man.


Glucocorticoid binding sites in rat brain: subcellular and anatomical localizations.


Altered action of parathyroid hormone by chlorpropamide.


Charge to the community: some early effects of a state hospital system's change of policy.


The principle of energy transduction in the cytochrome c oxidase region of the respiratory chain.


Energy coupling in cytochrome oxidase.

Energy coupling in cytochrome oxidase.


Enzymic generators of membrane potential in mitochondria.


Conduction within the cytochrome c molecule.


Heme-heme interaction between the cytochromes of the mitochondrial respiratory chain.


Solid state physical mechanisms of biological energy transduction.


Evaluation of the chemiosmotic interpretation of active transport in bacterial membrane vesicles.


The function of cytochrome c.

The function of cytochrome c.


The separation of electrons and protons during electron transfer: the distinction between membrane potentials and transmembrane gradients.


Irreversibility of the alteration in RNA transport induced by an azo-dye carcinogen in the rat liver.


Studies on a possible phosphoryl-enzyme intermediate in the catalytic reaction of yeast phosphoglycerate kinase.


Choline kinase and choline phosphotransferase in developing fetal rat lung.


The incorporation of glycerol into mitochondrial lipids during neonatal renal compensatory growth.


A functional acetylcholine receptor in the human erythrocyte.


Formation of mengovirus-like particles in cell-free extracts from virus-infected cells.


In vivo covalent binding of 14CCl4 metabolites in liver microsomal lipids.


Release of the inhibition of messenger RNA translation in extracts of interferon-treated Ehrlich ascites tumor cells by added transfer RNA.


The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue.


Use of a concanavalin A polymer to isolate right side-out vesicles of purified plasma membranes from eukariotic cells.


Isolation of the plasma membrane of a trypanosomatid flagellate: general characterisation and lipid composition.


Effect of ATP on the intermediary steps of the reaction of the (Na+ plus K+)-dependent enzyme system. 3. Effect on the p-nitrophenylphosphatase activity of the system.


A sensitive method for the bioassay of acetylcholine.


Mechanism of the inhibitory actions of some spasmogens on the bradykinin relaxation of the guinea-pig ileum.


A comparison of the utilization of thymine and thymidine for the synthesis of DNA-thymine in Novikoff hepatoma cells.


GH and ACTH secretion in insulin-induced hypoglycemia in normal children.


Effects of cell adhesion of the substratum on the growth of chick embryo fibroblasts.


Stereological analysis of the guinea pig pancreas. I. Analytical model and quantitative description of nonstimulated pancreatic exocrine cells. A stereological model which provides detailed quantitative information on the structure of the fasted, nonstimulated gland has been developed for the guinea pig pancreas. The model consists of morphologically defined space and membrane compartments which were used to describe the general composition of the tissue and the specific components of exocrine cells. The results are presented, where appropriate, relative to a cubic centimeter of pancreas, a cubic centimeter of exocrine cell cytoplasm, and to the volume of an average exocrine cell. The exocrine cells, accounting for 82% of the pancreas volume, consisted of 54% cytoplasmic matrix, 22% rough-surfaced endoplasmic reticulum (RER), 8.3% nuclei, 8.1% mitochondria, 6.4% zymogen granules, and 0.7% condensing vacuoles. Their total membrane surface area was distributed as follows: 60% RER, 21% mitochondria, 9.9% Golgi apparatus, 4.8% plasma membranes, 2.6% zymogen granules, 1.8% plasma membrane vesicles, and 0.4% condensing vacuoles. The application of this model to the study of membrane movements associated with the secretory process is discussed within the framework of an analytical approach.


Rapid changes in nucleoside transport induced by growth inhibitors. Studies with neoplastic mast cells. Aqueous extracts of murine embryonic or uterine tissue, or [(6)N]O(2)'-dibutyryl 3',5'-adenosine monophosphate (dbc-AMP) which were cytostatic for the murine mastocytoma P815Y in vitro also induced rapid changes in the incorporation of exogenous nucleosides into acid-insoluble material. However, these alterations were not a consequence of growth arrest. Different dose-response curves were obtained for cytostasis and inhibition of [(3)H]-nucleoside incorporation, and changes in [(3)H]thymidine uptake were detected within 15 min of treatment with the inhibitors. Also, there were differential effects of each inhibitor on the incorporation of (3)H-labeled thymidine, uridine, adenosine, or choline into acid-insoluble material.


Effects of cyclic AMP and Sephadex fractions of chick embryo extract on cloned retinal pigmented epithelium in tissue culture. The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low molecular weight (L) fractions of chick embryo extract. Cells grown in F-12 alone or in L medium formed compact epithelial sheets, whereas cells grown in H had a fibrocytic appearance and formed poorly organized monolayers. In H plus BcAMP, cell morphology was more epithelioid than in H alone, and generally the monolayers appeared more differentiated. Under each of these three culture conditions, 2 x 10(-4) M BCAMP retarded the increase in cell number and decreased the final number of cells per culture dish, but had little effect on plating efficiency. BcAMP also increased the rate of cell adhesion to a plastic substratum. Pigmentation was marked in cultures grown in F-12 or in L alone, but the addition of BcAMP dramatically reduced visible pigmentation. This effect was reversed when BcAMP was removed from the culture medium. Thus BcAMP modifies cell and colonial morphology, rate of cell accumulation, adhesive properties, and pigmentation of normal PE cells.


Characterization of a unique muscle cell line. A clonal cell line derived from a mouse neoplasm is described which shares many properties with smooth muscle. The cells have electrically excitable membranes capable of generating overshooting action potentials, and they contract both spontaneously and with electrical stimulation. They respond to the iontophoretic application of acetylcholine with a depolarizing response, and to norepinephrine with a hyperpolarizing response. Electron microscopy reveals that the cells have a morphology similar in many, but not all, respects to that of smooth muscle cells in vivo. The cells secrete soluble collagen-like molecules in addition to several proteins of undefined function. Finally, there is an increase in the specific activities of creatine phosphokinase and myokinase associated with increased cell density and the cessation of cell division.