Analytical fractionation of homogenates from cultured rat embryo fibroblasts. Homogenates of cultured rat embryo fibroblasts have been assayed for acid phosphatase, N-acetyl-beta-glucosaminidase, cathepsin D, acid deoxyribonuclease, cytochrome oxidase, NADH cytochrome c reductase, 5'-nucleotidase, inosine diphosphatase, acid pyrophosphatase, neutral pyrophosphatase, esterase, catalase, cholesterol, and RNA. The validity of the assay conditions was checked. Neutral pyrophosphatase is a readily soluble enzyme. Acid hydrolases, except acid pyrophosphatase, are particle-bound enzymes, which exhibit a high degree of structural latency. They are activated and solubilized in a parallel fashion by mechanical treatments and tensio-active agents. Catalase is also particle-bound and latent; activating conditions stronger than those for hydrolases are required to activate the enzyme. Acid pyrophosphatase, 5'-nucleotidase and inosine diphosphatase are firmly particle-bound, but not latent; they are not easily solubilized. In differential and isopycnic centrifugation, the latent hydrolases, cytochrome oxidase and catalase dissociate largely from each other; this suggests the occurrence of lysosomes and peroxisome-like structures besides mitochondria. The distribution patterns of 5'-nucleotidase and cholesterol are largely similar; digitonin influences their equilibrium density to the same extent; these two constituents are thought to be related to the plasma membrane. Inosine diphosphatase and acid pyrophosphatase are also partially associated with the plasma membrane, although some part of these enzymic activities probably belongs to other structures. NADH cytochrome c reductase is associated partly with the endoplasmic reticulum, partly with mitochondria.


The Mn2+-activated incorporation of inositol into molecular species of phosphatidylinositol in rat liver microsomes.


Copper and zinc levels in peripheral nerve tissues of rats with experimental carbon-disulphide neuropathy.


A study of the histological cell types of lung cancer in workers suffering from asbestosis in the United Kingdom.


What a dream is.

What a dream is.


Relapsing polychondritis and eye disease.


Imidazole catalysis of amino proton exchange in 2',3'-cyclic adenosine monophosphate. A general exchange mechanism.


Focal dermal hypoplasia.

Focal dermal hypoplasia.


Physical and chemical properties of rabbit muscle phosphofructokinase cross-linked with dimethyl suberimidate.


A homogeneous, thermostable deoxythymidine kinase from Bacillus stearothermophilus.


The phosphorylation of ribavirin by deoxyadenosine kinase from rat liver. Differentiation between adenosine and deoxyadenosine kinase.


Biosynthesis of cartilage procollagen. Influence of chain association and hydroxylation of prolyl residues on the folding of the polypeptides into the triple-helical conformation.


Ferrous porphyrins in organic solvents. II. Optical spectra and paramagnetic susceptibilities.


Structure of the primary acid rearrangement product of reduced nicotinamide adenine dinucleotide (NADH).


Glyceraldehyde-3-phosphate dehydrogenase catalyzed hydration of the 5-6 double bond of reduced beta-nicotinamide adenine dinucleotide (betaNADH). Formation of beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide.


Topography of 16S RNA in 30S ribosomal subunits. Nucleotide sequences and location of sites of reaction with kethoxal.


Purification and characterization of two forms of geranyl transferase from Ricinus communis.


Trypsin-modified isoleucyl transfer ribonucleic acid synthetase with partial retention of activity.


Structure of the major complex formed by interaction of phosphatidylcholine bilamellar vesicles and apolipoprotein-alanine (APO-C-III).


The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance.


The reactivity of conformationally modified cytochrome c.


Mechanism of the mutagenic action of hydroxylamine. VII. Functional activity and specificity of cytidine triphosphate modified with hydroxylamine and O-methylhydroxylamine.


Effects of temperature-induced phase changes in membranes on protein synthesis by bound ribosomes.


Ribosomal and soluble protein synthesis during a nutritional shift-up. Influence of cyclic AMP on beta-galactosidase activity.


Steroid-protein interactions. XXX. A progesterone-binding protein in the uterine cytosol of the pregnant guinea pig.


Enzymatic repair of x-ray-damaged DNA. I. Labeling of the precursor of a malonaldehyde-like material in X-irradiated DNA and the enzymatic excision of labeled lesions.


Low molecular weight DNA polymerase of rat ascites hepatoma cells.


Characterization of the 5'-terminus of murine leukemia virus RNA.


Effect of cyclic AMP on nucleoside metabolism. III. Effect on uridine incorporation in monkey (CV-1) cells.


ESR evidence for the radiation-induced breakage of the sugar-phosphate bonds in nucleotides: single crystal of deoxycytidine 5'-monophosphate.


Purification of pig heart mitochondrial membranes. Enzymatic and morphological characterization as compared to microsomes.


Chain ordering in liquid crystals. II. Structure of bilayer membranes.


Studies on Tetrahymena membranes: temperature-induced alterations in fatty acid composition of various membrane fractions in Tetrahymena pyriformis and its effect on membrane fluidity as inferred by spin-label study.


Some factors affecting the valinomycin-induced leak from liposomes.


A plasma membrane fraction from bovine adrenal medulla: preparation, marker enzyme studies and phospholipid composition.


The effect of phospholipase on the binding of asialoglycoproteins by rat liver plasma membranes.


Substrate-induced membrane potential changes in the perfused rat liver.


Biosynthetic pathways and turnover of individual biliary phosphatidylcholines during metabolism of (1,1-2H2)ethanol in the rat.


Biosynthesis of molecular species of phosphatidylcholines in bile, liver and plasma of rats given (1,1-2H2)ethanol.


Mono-acid diacylglycerols in human adipose tissue.


Lipid components of two different regions of an intestinal epithelial cell membrane of mouse.


The kinetics of NAD-linked isocitrate dehydrogenase from calf heart.


Protein kinases in Tetrahymena cilia. II. Partial purification and characterization of adenosine 3',5'-monophosphate-dependent and guanosine 3',5'-monophosphate-dependent protein kinases.


Studies on manganese-substrate complexes of arginine kinase from Panulirus longipes.


Effect of sulfhydryl reagents on the activity of histidinolphosphatase from Salmonella typhimurium and bakers' yeast.


Separation of multiple forms of cyclic nucleotide phosphodiesterases from rat brain by isoelectrofocusing.


Cyclic nucleotide phosphodiesterase in silkworm: purification and properties.


Identification of the thiol residues involved in modifications of pig heart lipoamide dehydrogenase by cupric ion and by iodoacetamide.


Oxidation of NADH by melanin and melanoproteins.


Regulation of purine metabolism in Schizosaccharomyces pombe. IV. Variations in the stability and kinetic parameters of amidophosphoribosyltransferase depending on growth phase and growth conditions.


EPR studies of nitric oxide complexes of turnip and horse-radish peroxidases.


A fluorescent probe for the coenzyme-induced structural changes in glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.


Binding of the estradiol receptor from calf uterus to the chromatin: active forms.


Tritium labeling of luteinizing hormone by reductive methylation.


The apoproteins of various size classes of human chylous fluid lipoproteins.


Magnetic susceptibility of lacquer tree laccase.


Metabolism of phytane in rats.

Metabolism of phytane in rats.


Multiple molecular forms of phosphoprotein phosphatase. II. Dissociation and activation of phosphorylase phosphatase from rabbit skeletal muscle.


The cleavage of the carbon--cobalt bond in vitamin B12 and coenzyme B12 by reduction with electrons in gamma-irradiated frozen solutions.


The pancreatic beta-cell recognition of insulin secretagogues. XIII. Effects of sulphydryl reagents on cyclic AMP.


Subcellular localization and some properties of the adenylate cyclase activity of the yeast, Saccharomyces cerevisiae.


Cyclic AMP and thyroglobulin biosynthesis in the experimental rat thyroid tumor.


The isolation and partial characterization of a glycoprotein isolated from human gastric aspirates and from extracts of gastric mucosae.


The effect of isoproterenol and its analogs upon adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate levels in mouse parotid gland in vivo. Relationship to the stimulation of DNA synthesis.


The actions of insulin and glucagon on glucose metabolism and on related enzyme activities in the isolated perfused rat liver.


Glycoprotein biosynthesis in liver mitochondria. II. Mitochondrial localization of mannosyltransferase.


The effect of pressure on the phase diagram of mixed dipalmitoyl-dimyristoylphosphatidylcholine bilayers.


Androgen receptors in rat ventral prostate microsomes.


Cycloheximide inhibition of hypoxanthine transport cultured cells.


Effect of several "specific" chemical reagents on the Na+, K+ and leakage currents in voltage-clamped single nodes of Ranvier.


Effect of chemical modifiers of passive permeability on the conformation of spin-labeled erythrocyte membranes.


Effect of theophylline and Na+ on methionine influx in Na+-depleted intestine.


Temperature sensitivity of the assembly process of the enveloped bacteriophage phi6.


The methylation of transfer RNA in Escherichia coli.


Properties of the homogeneous main band DNA from the human genome.


Is there a first night effect? (a revisit).


Longitudinal study of the effects of lithium carbonate on the sleep of hospitalized depressed patients.


Spontaneously recalled verbal material and its linguistic organization in relation to different stages of sleep.


The intracellular phase of fat absorption.


Simplified radioimmunoassay of angiotensin II.


Some hydrodynamic and optical properties of polyribonucleotides.


On site heterogeneity in sturgeon muscle GPDH: a kinetic approach.


Nucleotide and serotonin metabolism in platelets with defective secondary aggregation.


Criteria for the differentiation of lymphoid cell lines.


Further observations on the virological studies on the epidemic of conjunctivitis in Calcutta.


Isolation of virus from the urine, conjunctiva and throat of smallpox cases.


Role of acetylcholine in the mechanism of nervous autoregulation of the heart.


Role of the limbic structures in the mechanism of action of glucocorticoids and estrogens on hypothalamic control of pituitary adrenocorticotropic and gonadotropic functions.


A rare case of hidradenoma papilliferum: report of a case and review of the literature.


The mechanism of action of ACTH on the adrenal cortex.


Vascular reactivity in spontaneously hypertensive normotensive and hypotensive rats. 1 Three strains of rats spontaneously hypertensive (HRS), normotensive (NS) and hypotensive (HOS), were selected by repeated inbreeding.2 In order to explain the different blood pressure levels observed, we have studied their vascular reactivity to noradrenaline and angiotensin II. Experiments were performed in anaesthetized ganglion-blocked, vagotomized rats.3 The reactivity to noradrenaline was significantly higher in HRS than in NS and HOS rats.4 The reactivity to angiotensin II appeared identical in the three strains used.


Effects on plasma and brain tryptophan in the rat of drugs and hormones that influence the concentration of unesterified fatty acid in the plasma. 1 The effects on tryptophan distribution and metabolism of drugs altering plasma unesterified fatty acid (UFA) concentration were investigated in the rat.2 UFA and plasma free (i.e. ultrafilterable) tryptophan altered in the same direction.3 Catecholamines and L-DOPA increased both plasma UFA and free tryptophan. L-DOPA also increased brain tryptophan and 5-hydroxyindoleacetic acid (5-HIAA) but decreased brain 5-hydroxytryptamine (5-HT).4 Aminophylline increased plasma UFA and free tryptophan and also brain tryptophan, 5-HT and 5-HIAA. Food deprivation had qualitatively similar effects.5 Insulin decreased plasma UFA and free tryptophan in both fed and food-deprived rats. However, while in fed rats these changes were associated with small decreases of brain indoles, in food-deprived animals small increases occurred.6 Nicotinic acid had only small effects in fed rats but it opposed both the UFA and indole changes in food-deprived animals. Total plasma tryptophan increased in nicotinic acid treated, food-deprived rats.7 There was a tendency towards inverse relations between changes of plasma free and total tryptophan.8 The results suggest that drugs which influence plasma UFA through actions on cyclic AMP thereby alter the binding of tryptophan to plasma protein and that this leads to altered distribution and metabolism of tryptophan.


Mechanism of the pro-inflammatory activity of sympathomimetic amines in thermic oedema of the rat paw. 1 Thermic oedema induced by heating rat paws at 46.5 degrees C was potentiated by local injection of adrenaline, noradrenaline or high doses of isoprenaline. The pro-inflammatory effect of sympathomimetic amines was antagonized by phenoxybenzamine or phentolamine but not by propranolol.2 The subcutaneous space of heated rat paws was perfused with Tyrode solution and the perfusate collected and assayed for bradykinin, bradykininogen, kinin-forming activity and kininase activity. When adrenaline (0.5 mug/ml) was included in the perfusion fluid, kininase activity of the perfusate was increased by 76% and free bradykinin reduced by 46%.3 Increased vascular permeability induced by injection of bradykinin or kallikrein was reduced by adrenaline or noradrenaline, but isoprenaline had no significant effect.4 Pretreatment with soya bean trypsin inhibitor (SBTI) or heparin did not antagonize the pro-inflammatory effect of adrenaline or thermic oedema per se.5 Potentiation of thermic oedema similar to that induced by sympathomimetic amines was obtained by injecting paws with vasopressin prior to heating, or by applying a ligature to stop blood flow to the paw for the first 15 min of heating.6 Thermistor probes inserted beneath the paw skin showed that sympathomimetic amines increased the internal temperature of heated paws. This was significant, as small changes in temperature had a marked effect on the development of thermic oedema.7 It is suggested that sympathomimetic amines potentiate thermic oedema of rat paws heated at 46.5 degrees C by reducing blood flow to the paw, thereby causing a greater rise in paw temperature and consequently greater injury.


Effect of ethanol on the oxidative metabolism of tryptamine by rat liver homogenate. 1 The effect of a wide range of ethanol concentrations (v/v) on indoleacetic acid (IAA) formation from the oxidative deamination of tryptamine was studied in vitro, in rat whole liver homogenate.2 IAA production was inhibited progressively by ethanol in concentrations between 0.01% to 0.2%, but the inhibition declined when the ethanol concentration was increased further to 6%.3 Ethanol-induced inhibition of IAA formation was only partially reversed by excess aldehyde dehydrogenase, whereas reductions in IAA formation were completely prevented by pyrazole or ethanol (6% and 10%) itself.4 Excess nicotinamide adenine dinucleotide failed to alter the inhibitory effect of ethanol and no evidence was obtained for inhibition of monoamine oxidase by ethanol or its metabolite, acetaldehyde.5 We conclude that ethanol indirectly inhibits IAA production as a result of oxidation of ethanol by alcohol dehydrogenase, during which the oxidative metabolism of tryptamine is shifted towards the reductive pathway, thus favouring the formation of tryptophol in place of IAA.


Dose-response curves for angiotensin II and synthetic analogues in three types of smooth muscle: existence of different forms of receptor sites for angiotensin II. 1 The dose-response curves for angiotensin II and four analogues in rat colon and uterus, and in rabbit aorta, were obtained.2 The ED(50) values of angiotensin II as well as the ED(50)s and intrinsic activities of angiotensin analogues varied between the three organs.3 The interaction of two competitive antagonists with angiotensin II receptors was also examined. The K(I) values obtained with both antagonists on the uterus differed from the values on the other tissues.4 It is concluded that angiotensin II receptors may be different in the three organs examined.


Letter: B-lymphocytes as target cells for E.B. virus transformation.


Editorial: Screening for hyperlipidaemia in childhood.


What could the G.P. treat at home--with proper support?


Letter: E.B. virus and treatment of lymphomas.


Paradoxical sleep: prolonged augmentation following learning.


Comparative measurements of the crystallinity of callus and compact bone by electron spin resonance spectrometry.


Evidence for the biosynthetic difference between isolated mitochondria and microsomes from guinea-pig and rat liver regarding lysophosphatidic acid, phosphatidic acid, CDP-diglyceride, phosphatidylglycerol, and cardiolipin.


Biosynthesis of spin-labelled lipids and their use in the study of biological membranes: isolation and characterization of membrane-bound spin-labelled sn-3-phosphatidic acid-2-3H prepared by enzymatic incorporation of spin-labelled stearic acid into sn-glycero-3-phosphate-2-3H.


Lipid and temperature dependence of membrane-bound ATPase activity of Acholeplasma laidlawii.


Isolation of dimethylallylpyrophosphate: umbelliferone dimethylally transferase from Ruta graveolens.


Succinate dehydrogenases of Mucor rouxii and Penicillium duponti.


Localization of phosphate-independent glutaminase in the brush border of rat kidney cortex.


Mutation test of 2,4,5-trichlorophenoxyacetic acid on Drosophila melanogaster.


Time differential in labelling of different monosaccharides into serum lipoproteins in rats.


Mixed tumor of the nasal septum.

Mixed tumor of the nasal septum.


Regulation of pancreatic acinar function: effects of cyclic AMP, dibutyryl cyclic AMP, and theophylline in vitro.


Intestinal vasoconstriction following diuretic-induced volume depletion: role of angiotensin and vasopressin.


The effect of intravenous infusions of angiotensin and noradrenaline on systolic blood pressure and critical opening pressure of tail vessels in conscious rats.


Stimulation of nerve fiber growth from cultured sympathetic explants.


Dantrolene sodium: effects on crustacean muscle.


Negative cardiac inotropic effects of calcium ion and of N-2-hydroxyethylpiperazine-N' -2-ethanesulfonic acid (HEPES) buffer at normal and low sodium concentration.


Catecholamine binding and the turnover number of adenylate cyclase.


Surgical treatment of Crohn's disease.

Surgical treatment of Crohn's disease.


Criteria for the post-hospital adjustment of mental patients in sheltered settings.


A quantitative ultrastructural study of the pleomorphic adenoma (mixed tumor) of human minor salivary glands.


Pseudomembranous colitis associated with antibiotic therapy - an emerging entity. Two cases of pseudomembranous colitis are presented. The first patient had been treated with novobiocin-tetracycline and penicillin, and two weeks later developed severe fulminating diarrhea with ascites and bilateral pleural effusions which did not respond to intravenous ACTH. Subsequently she underwent subtotal colectomy and made a rapid and complete recovery. The second patient developed severe diarrhea two weeks after a 10-day course of clindamycin. She was treated with intravenous ACTH, oral Lactobacillus and a fecal enema and made a complete recovery.These cases reconfirm the importance of antibiotics as etiologic agents in this disease. They also stress the classic sigmoidoscopic and histologic findings that should facilitate prompt and rapid diagnosis.


Localized fibrous tumors of pleura: a light and electron microscopic study.


Cell kinetic studies in patients with small cell carcinoma of the lung.


Angiolymphoid hyperplasia with eosinophilia in the skin.


Urinary cholesterol--VI. Its excretion in women with inoperable inflammatory carcinoma of the breast.


Concurrence of carotid body tumor and pheochromocytoma.


Half-life of N-acetylneuraminic acid in plasma membranes of rat liver and Morris hepatoma 7777.


A difference between B10.D2-n and B10.D2-o strains of mice in rates of irradiation-induced leukemogenesis.


Estrogen receptor content and hormone-responsive growth of mouse mammary tumors.


Dietary effects on stearyl coenzyme A desaturase in Morris hepatomas.


An ultrastructural study of C-type virion assembly in mouse cells.


Comparison of daunorubicin (NSC-83142) with adriamycin (NSC-123127) in the treatment of late-stage childhood solid tumors.


Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.


Further investigation of a variant of the placental alkaline phosphatase in human hepatic carcinoma.


Evaluation of intravenous allopurinol (NSC-1390) in pediatric neoplasia.


Demonstration of glucose 6-phosphatase activity in the oval cells of rat liver and the significance of the oval cells in azo dye carcinogenesis.


Tumor induction in the progeny of mice receiving 4-nitroquinoline 1-oxide and N-methyl-N-nitrosourethan during pregnancy or lactation.


Alteration of tumor response in rat liver by carbon tetrachloride.


The effect of benzo(alpha)pyrene on the basal and isoproterenol-stimulated levels of cyclic adenosine 3',5'-monophosphate in mouse epidermis.


Incorporation of precursors and inhibitors of nucleic acid synthesis into hepatomas and liver of the rat.


A leukemic lymphoproliferative diseases in A-J mice following inoculation with blood from patients with acute infectious hepatitis.


Phorbol-12-myristate-13-acetate effect on cyclic adenosine 3',5'-monophosphate levels in mouse skin and inhibition of phorbol-myristate-acetate-promoted tumorigenesis by theophylline.


In vitro growth of an attenuated mouse leukemia virus.


The possible correlation of growth rate and expression of transformation with temperature-dependent modification in high-molecular-weight membrane glycoproteins in mammalian cells transformed by a wild-type and by a thermosensitive mutant of avian sarcoma virus.


Enhancement of simian virus 40 transformation and integration by 4-nitroquinoline 1-oxide.


In vivo inhibition of tumor growth by cyclic adenosine 3',5'-monophosphate derivatives.


Cation preferences for poly(rC)-oligo(dG)-directed DNA synthesis by RNA tumor viruses and human milk particulates.


Periodate oxidation of L-iduronic acid residues in dermatan sulphate.


Alkaline and smith degradation of oxidized dermatan sulphate-chondroitin sulphate copolymers.


Periodate oxidation, acid hydrolysis, and structure-activity relationships of human-pituitary, follicle-stimulating hormone and human chorionic gonadotrophin.


Chemical synthesis of P1-2-acetamido-2-deoxy-alpha-D-glucopyranosyl P2-dolichyl pyrophosphate.


The attachment of poly(ribitol phosphate) to lipoteichoic acid carrier.


A micromethod for the determination of 5-(adenosine 5'-pyrophosphoryl)-D-ribose (adenosine diphosphoribose-(5)) in biological extracts.


Immunochemical studies on the specificity of soybean agglutinin.


Fine structural localization of nucleoside triphosphatase and acid phosphatase activity in Trichomonas vaginalis Donné.


Cephalopod integument: the ultrastructure of Kölliker's organs and their relationship to setae.


Investigations on turnover of adrenocortical mitochondria. II. Effect of chronic treatment with ACTH on the 3H-thymidine incorporation into rat zona fasciculata mitochondria.


Freeze-fracture studies on the cockroach hemocyte membrane complex: symmetric and asymmetric membrane particle distributions.


Ultrastructural and cytochemical localization of the storage sites of monoamines in the paraventricular organ of Xenopus laevis tadpoles.


Pigment movements in fish melanophores: morphological and physiolgical studies. IV. The effect of cyclic adenosine monophosphate on normal and vinblastine treated melanophores.


Studies on the juxtaglomerular apparatus. III. Quantitative morphology after treatment with deoxycorticosterone (DOC).


Metabolism of cyclic adenosine 3':5'-monophosphate in somatic cell hybrids.


The release of secretory products from the corticotrophic cells of Salmo gairdneri in vitro.


Nuclear protein changes during the nitrosamine-induced carcinogenesis of rat liver.


Studies on viomycin. VII. Oxidative modifications of viomycin.


Studies on peptides. XLVI. Synthesis of human type corticotropin-like intermediate lobe peptide (CLIP).


Studies on peptides. XLV. Synthesis of the bovine type corticotropin-like intermediate lobe peptide (CLIP).


Synthesis of D,L-erythro-sphingomyelins.


Vascular effects of procaine amide in the dog. Predominance of the inhibitory effect on ganglionic transmission.


Characteristics of hypertension in the black population.


Adolescent hypertension. II. Characteristics and response to treatment.


Altered sedimentation behavior and ultrastructure of platelets in hyperlipidemia.


Transport of beta-carotene in serum of individuals with carotenemia.


Asthma due to inhaled chemical agents: ampicillin, benzyl penicillin, 6 amino penicillanic acid and related substances.


Development and standardization of radioimmunoassays for prostaglandins E, F, and A.


Isozymes of human alpha-L-fucosidase detectable by starch gel electrophoresis.


Further characterization of the serum pre-alpha-lipoprotein.


Control of plasma aldosterone in supine anephric man.


Evidence for an underlying adrenocortical abnormality in hirsute women.


A syndrome of polydactyly-syndactyly and triphalangeal thumbs in three generations.


Lp(a) lipoprotein and pre-beta1-lipoprotein in patients with coronary heart disease.


Aldosterone and its regulation during diuresis in patients with gross congestive heart failure.


Changes in the fatty acid composition of plasma lipoproteins during administration of Intralipid to a patient with essential fatty acid deficiency.


Leishmania donovani and L. mexicana: production of the excretion factor.


Composition of very low density lipoproteins from cholesterol fed animals.


Chymotrypsins.

Chymotrypsins.


Eggshell calcification with systemic sarcoidosis.


Granular cell myoblastoma of the bronchus: a new case, 12-year followup report, and review of the literature.


Uptake of exogenous 3H-1,2-testosterone in the hypothalamus, endocrine glands and sex-accessory organs in neonatally castrated and androgenized male rats.


Correlation between the activities of enzymes involved in glucose oxidation in corpus luteum and the concentration of sex steroids in systemic plasma during the reproductive cycle of the guinea pig.


Ontogeny of rat hepatic glucocorticoid receptors.


Preparation of biologically active 125I-TSH.


Functional and morphological observations on rat adrenal zona glomerulosa cells in monolayer culture.


The effects of hypothyroidism and 2,4-dinitrophenol on growth hormone synthesis.


Progesterone "receptor" in human endometrium.


Maternal and fetal plasma progesterone, cortisol, testosterone and 17beta-estradiol in preparturient sheep: response to fetal ACTH infusion.


Interaction of lithium with vasopressin-sensitive cyclic AMP system of human renal medulla.


In vitro testosterone biosynthesis in the human fetal testis. II. Stimulation by cyclic AMP and human chorionic gonadotropin (hCG).


5Alpha-reduction and binding of testosterone in androgen-dependent and -independent mouse mammary tumors.


Chicken insulin: radioimmunological characterization and enhanced activity in rat fat cells and liver plasma membranes.


Inhibitory and facilitatory hypothalamic areas mediating ACTH release in the cat.


Stimulation of angiotensinogen release by hemodilution in the dog.


Multiple sclerosis: reticulum cell sarcoma of the nervous system in a patient treated with immunosuppressive drugs.


Changes in cell mediated immune functions by growing tumor.


Chromosome studies on 14 near-diploid carcinomas of the ovary.


Interferon production by leukocytes cultures from neoplastic patients.


Antibiotic combinations of gram-negative infections in patients with cancer.


Empirical antibiotic therapy in febrile patients with neutropenia and malignant disease.


Advances in the management of Pseudomonas aeruginosa infections in cancer patients.


Pseudomonas septicemia: incidence, epidemiology, prevention and therapy in patients with advanced cancer.


Immune response to adenovirus 12-induced tumour antigens, as measured in vitro by the macrophage migration inhibition test.


In vivo effect of adenosine 3', 5'-monophosphate on Ehrlich ascites tumour cells.


Estrogen receptors in human breast cancer.


Specific estrogen receptor of the DMBA-induced mammary carcinoma of the rat and its estrogen-requiring molecular transformation.


Interaction of activated estradiol-receptor complex and chromatin in isolated uterine nuclei.


Interaction of vasopressins and linear N-terminal ACTH fragments in the induction of natriuresis.


Isolation of a tridecapeptide from Natriuretic fractions of bovine posterior pituitary.


Adrenal secretion of estrogens, glucocorticosteroids and mineralocorticosteroids in the dog.


Selective activation of mouse T and B lymphocytes by periodate, galactose oxidase and soybean agglutinin.


The anti-virus activity of double-stranded RNA in the presence of antibody to double-stranded RNA.


The influence of volume loading on the inotropic effect of Ro 7-2956.


Antidiuretic hormone and renin in rats with diabetes insipidus.


Cold potentiation of neuromuscular transmission in the avian biventer cervicis muscle.


Effects of droperidol on neuromuscular transmission and muscle membrane.


Release of prostaglandins from the rabbit renal medulla.


Antibody-induced redistribution of normal and tumor associated surface antigens.


Rapid isolation of nucleoli from detergent purified nuclei of various tumor and tissue culture cells.


Isolation of temperature-sensitive mutants from murine leukemic cells (L5178Y).


Isolation of Kupffer cell lysosomes, with observations on their chemical and enzymic composition.


Influence of cell cycle and cell movement on the distribution of intramembranous particles in contact-inhibited and transformed cells.


In vitro phosphorylation of chicken erythrocyte histone by various protein kinases: absence of phosphorylation from F1 histone.


Proteases as mitogens. The Effect of trypsin and pronase on mouse and human lymphocytes.


Complementation in hybrid cells derived from mutagen-induced mouse clones deficient in hypoxanthine-guanine phosphoribosyl-transferase activity.


Introduction of nuclei and micronuclei into cells and enucleated cytoplasms by Sendai virus induced fusion.


Relation of activation of neurones in the pons and medulla to brain-stimulation reward.


Cerebellar Purkynĕ cell responses to inputs from sensorimotor cortex.


Schistosoma mansoni: penetration apparatus and epidermis of the miracidium.


Somatotopically organized inputs from fore- and hindlimb areas of sensorimotor cortex to cerebellar Purkynĕ cells.


Effect of a catatoxic steroid pregnenolone-16alpha-carbonitrile, on rat liver microsomal subfractions.


Isolation of rat liver cells containing concanavalin-A receptor sites.


A comparative study of SV40-transformed fibroblast plasma membrane proteins labelled by enzymatic iodination or with trinitrobenzene sulfonate.


Hepatic action of vasopressin: lack of a role for adenosine-3',5'-cyclic monophosphate.


Glycogen synthetase kinase 2 (GSK 2); the identification of a new protein kinase in skeletal muscle.


Hormone-stimulated cAMP production in human placenta perfused in vitro.


Inhibition of lipolysis in bovine adipose tissue by butyrate and beta-hydroxybutyrate.


Conformational changes at membranes of target cells induced by the peptide hormone angiotensin. A spin label study.


NAD levels in 3T3 cells during exponential growth and density-dependent inhibition of growth.


Nicotinamide 1, N6-ethenoadenine dinucleotide, a coenzyme for glutamate dehydrogenase.


A receptor for testosterone in mature rat testes.


The effect of catecholamine analogues upon amylase secretion from the mouse parotid gland in vivo:relationship to changes in cyclic AMP and cyclic GMP levels.


The amino acid sequence of cytochrome c from Solanum tuberosum (potato).


The carbohydrate composition of submitochondrial fractions from rat liver.


A peptic phosphopeptide from the active site of pea seed nucleoside diphosphate kinase.


Specific spin-labelling of the energy-coupling system of mitochondria.


Synthesis of cytochrome oxidase components in isolated mitochondria of Neurosora crassa.


Activation of adenyl cyclase in the early phase of germination.


Transcription of the Col E1 genome in colicinogenic E. coli strains during induction with mitomycin C.


Defective protein phosphorylation in membranes of hereditary spherocytosis erythrocytes.


EPR detection of deuteriochlorophyll cation-radicals arising at its photoreaction with quinone at low temperature.


Energy transduction in photosynthetic bacteria. The nature of cytochrome C oxidase in the respiratory chain of Rhodopseudomonas capsulata.


Reaction of cytochrome C oxidase with CO: involvement of the invisible copper.


Molecular weight determination of the chymotrypsin-like protease of the larvae of the hornet, Vespa orientalis, by affinity labeling of the active center.


LH-RH-receptor interaction is inhibited by des-His--2-des-Gly--10-LH-RH-ethylamide.


Vitamin D: the vitamin and the hormone.

Vitamin D: the vitamin and the hormone.


Present knowledge of the role of chromium.


Lesions associated with impacted teeth.

Lesions associated with impacted teeth.


The nature of pain.

The nature of pain.


Xanthomatosis and hyperlipoproteinemia; a review.


A developmental study on the capacity of rabbit granulosa cells to respond to trophic hormones and secrete progesterone in vitro.


Testosterone-protein binding in cytosol of rat anterior hypophysis in vitro: evidence for a specific receptor.


Development of phosphodiesterase activity in the chick retina.


Increased hepatic gluconeogenesis without a rise of glucagon secretion in rats fed a high fat diet.


Dietary and drug treatment of hyperlipidemia in diabetes.


Quantitative study on thromboplastin in various strains of Yoshida ascites hepatoma cells of rat.


Inhibitory effect of Capsella bursa-pastoris on hepatocarcino-genesis induced by 3-methyl-4-(dimethylamino)azobenzene in rats.


Dual infection of hamsters with Adenovirus type 12 and type 6.


Selective distribution of the radioactivity in nerve tissues of the mouse after intravenous administration of 14C-labeled N-methyl-N-nitrosoguanidine.


Microsomal acetylesterase activity in tumor-bearing rats.


Collision tumor of the stomach. Report of two cases.


Effects of cholera toxin and phosphodiesterase inhibitors on fluid transport and cyclic adenosine 3',5'-monophosphate concentrations in rabbit gallbladder.


Endoscopic retrograde cholangiopancreatography in the diagnosis of jaundice associated with ulcerative colitis.


Cytodiagnosis of pancreatic malignant tumors by aspiration, under direct vision, using a duodenal fiberscope.


Further studies on the hormonal control of melanophores and iridophores isolated from bullfrog tadpoles.


A five-step approach to antimicrobial medication. 2. Urinary tract infections.


Electron microscopic study on the jejunal mucosa in human cholera. Small intestinal mucosa obtained from six fasting patients with cholera by a peroral biopsy technique was studied with the electron microscope. The cultures of their rectal swabs were all positive for Vibrio cholerae. In the absorptive cells, large pseudopod-like cytoplasmic processes with deformed microvilli or without microvilli (blebs) projected into the intestinal lumen from the apical cell surface, interrupting the microvillous border. In the crypts some of the undifferentiated crypt cells possessed pseudopod-like cytoplasmic projections which extended into the crypt lumen. The swelling of mitochondria, the disappearance of their cristae, an increase in the number of vesicles of the Golgi apparatus, and the dilatation and vesiculation of endoplasmic reticulum were observed in the epithelial cells. The apical portion of the cells became oedematous in some of the epithelial cells. Cytoplasmic fragments enclosed by a plasma membrane, desquamated epithelial cells, cytoplasmic organelles, and secretory granules were lying free in the intestinal lumen.Marked irregular widening of the interepithelial spaces in the jejunal mucoa was observed at the midvillous area. Many cystic vacuoles were present in the cytoplasm of epithelial cells. The possibility of fluids being transported from blood vessels to intestinal lumina through the interepithelial spaces, and the significance of these pathological findings in human cholera are discussed in this study.


Histochemical studies on the mucous membrane of the jejunum after vagotomy and pyloromyotomy in white rats.


Ultrastructure of renal glomerulus under conditions of hypercalcemia induced with vitamin D3.


Comparative studies on the behavior of C cells in the rat thyroid under conditions of hypercalcemia induced with vitamin D3 and calcium gluconate.


Oxidative phosphorylation in a yeast mutant lacking alpha-ketoglutarate dehydrogenase activity.


Recent developments in the chemistry of penicillins.


Structure, chemistry, and biosynthesis of the melanins.


Interactions of 17 beta-estradiol and L-epinephrine on the rat uterus.


Study of the effects of morphine on adrenal corticosteroids, ascorbic acid and catecholamines in unanaesthetized and anaesthetized cats.


Molecular and catalytic properties of unspecific carboxylesterases (EC 3-1-1-1).


Stereospecificities in the metabolic reactions of the four isomeric sphinganines (dihydrosphingosines) in rat liver.


Studies on the B-protein of human serum beta-lipoprotein using SDS-polyacrylamide gel electrophoresis.


Magnetic resonance studies on the binding of manganese to D-fructose-1,6-diphosphate 1-phosphohydrolase (hexose-1,6-diphosphatase).


Metabolic transformation of colchicine. I. The oxidative formation of products from colchicine in the Udenfriend system.


Sphingosine kinase in blood platelets.

Sphingosine kinase in blood platelets.


Different half-lives of nicotinamide and adenine moieties of NAD in HeLa S3 cultures.


Expression of the mitochondria genome in wild type and in extracellular mutants of Neurospora crassa.


The orientation of serologically active phospholipids in mitochondrial membranes, I.


The orientation of serologically active phospholipids in mitochondrial membranes, II. Studies in vitro with a complement fixation reaction.


Mitochondrial H2O2 formation at site II.


Gangliosides of extraneural organs.

Gangliosides of extraneural organs.


Continuous synthesis of NAD in a nuclear column.


Studies on ADP-ribose ribosylation in rat liver nuclei.


Radiographic determination of excessive bone loss in Alaskan Eskimos.


The distribution of some erythrocyte and serum enzyme group systems in the Mahishyas and the Muslims of 24-Parganas, West Bengal.


Metabolism of cholesterol in rats under dietary necrosis.


Certain biochemical changes in the root meristem cells of Vicia faba treated with mitomycin C.


A dipole mechanism of nervous transmission.


Effect of age on the composition of major phospholipids of mycobacteria.


Immunological relationship between antigens induced by leukemia virus & mammary tumor virus in an inbred mouse strain.


Antisera to carcinoembryonic antigen: removal of persistant impurities.


Jaagziekte & maedi of sheep & goats transmitted in laboratory animals.


Recurrent cutaneous herpes simplex in hairless mice. Passively immunized hairless mice were inoculated cutaneously with herpes simplex virus. Thirty-nine days later, when the primary cutaneous lesions had completely healed, the mice were treated subcutaneously with prednisone. Within 12 to 30 days after starting prednisone treatment, herpesvirus was recovered by skin swabs from 12 of 71 (17%) of the treated mice. This new model has potential application for understanding and treating recurrent cutaneous herpes infections.


Stimulation of peripheral blood lymphocytes by herpes simplex virus in vitro. A method for stimulating sensitized lymphocytes by inactivated herpes simplex virus was established by using cultures of washed whole blood cells. The development of the immune response of herpes simplex virus-infected guinea pigs was examined at different times in the 4-month period after infection. Humoral and cellular immune responses were compared in individual animals by measuring lymphocyte stimulation ratios and neutralizing serum antibodies.


Relationship of Ehrlichia canis-infected mononuclear cells to blood vessels of lungs. The lung tissue of six dogs with ehrlichiosis and two control dogs was examined with the electron microscope. Mononuclear cells containing inclusions (morulae) of Ehrlichia canis were adhered at one or more sites to the luminal surfaces of endothelial cells of arterioles or capillaries by way of interdigitations or areas of adherence, or an endothelial cell-bound mononuclear cell was chained to another parasitized or nonparasitized mononuclear cell in the lumen. The bifurcation of arterioles was the most common site at which mononuclear cells clung to the endothelium. Cell-free bodies (morulae), enclosed by a single membrane, were present in the lumens of arterioles. Such cell-free bodies were swollen and vesiculated as compared with intracytoplasmic inclusions (morulae) in mononuclear cells.


Differentiation of strains of infectious bovine rhinotracheitis virus by neutralization kinetics with late 19S rabbit antibodies. Two vaccine, two respiratory (infectious bovine rhinotracheitis [IBR]), and two genital (infectious pustular vulvovaginitis [IPV]) strains of infectious bovine rhinotracheitis virus were compared by neutralization kinetics using late 19S antibody (AB). The two vaccine strains were indistinguishable from one another, but were neutralized far more rapidly than the other four strains when either anti-IBR or anti-IPV 19S AB was used. The two IPV strains were indistinguishable from one another, but were neutralized significantly more rapidly than the two IBR strains when anti-IBR 19S AB was used. The 2 IBR strains were neutralized at a similar rate with the latter globulin preparation. Almost identical results were obtained with anti-IPV 19S AB, except that one IPV strain was neutralized at a rate similar to the IBR strains. However, when early and late rabbit 7S AB were used, IBR strains could not be distinguished from IPV strains by neutralization kinetics. Preliminary experiments indicated that both early and late 19S rabbit antibodies neutralized the homologous strain more rapidly than the heterologous strain, but the difference was more noticeable with late 19S AB. It was also determined that neutralization of IBR-IPV virus by specific early and late 19S rabbit AB and early 7S rabbit AB was markedly enhanced by guinea pig complement. Neutralization of this virus by late 7S AB, however, was only slightly enhanced by complement. These results suggest that vaccine strains of IBR-IPV virus may be distinguished by neutralization kinetics with late 19S rabbit AB, and that genital and respiratory strains may possibly also be distinguishable with some 19S AB preparations.


Equine infectious anemia virus from infected horse serum. Equine infectious anemia virus was purified from infected horse serum samples. Electron microscope observation on negatively stained preparations of purified virus showed roughly spherical particles sized between 100 and 200 nm in diameter. In disrupted particles, an envelope was visible but no internal structure could be resolved. Since the purified virus fraction had a strong antigenic activity to antiserum in immunodiffusion reaction, these particles are thought to be the causative virus of equine infectious anemia.


Comparison of the action of Escherichia coli enterotoxin on the thymocyte adenylate cyclase-cyclic adenosine monophosphate system to that of cholera toxin and prostaglandin E1. Mouse thymocytes were used to compare mechanisms by which Vibrio cholerae and heat-labile Escherichia coli enterotoxins activate the adenylate cyclase-cyclic adenosine monophosphate (AMP) system. Both enterotoxins had their time-delayed increase in cyclic AMP neutralized by antisera to V. cholerae or E. coli enterotoxin, blocked by low concentrations of ganglioside G(M1), and destroyed by prior heating. Enterotoxin activation of adenylate cyclase was similarly affected. By contrast, prostaglandin E(1)-mediated increases in cyclic AMP were not affected by specific antitoxins or gangliosides. Combination of maximal stimulatory doses of both enterotoxins did not produce additive increases in cyclic AMP. Wash experiments suggested that both enterotoxins bind rapidly and tightly to thymocytes at 37 C. However, lowering the incubation temperature to 8 C reduced the affinity of E. coli enterotoxin but not cholera toxin for thymocytes. Results suggest that heat-labile E. coli enterotoxin and cholera enterotoxin may activate the same adenylate cyclase enzyme by similar mechanisms.


Comparative studies of the Smith and Raynaud strains of murine cytomegalovirus. A number of the biological, biophysical, and immunological parameters of the Smith and Raynaud strains of murine cytomegalovirus were compared. The two strains were found to be indistinguishable.


Neutralizing activity in isolated serum antibody fractions from visna-infected sheep. The neutralization technique was used to detect specific antibodies against visna virus in isolated immunoglobulin (Ig) G(1), IgG(2), and IgM fractions of visna-infected sheep serum. The neutralizing antibodies were present chiefly in the IgG(1) class. Low amount of activity was demonstrated in the IgM class, but no significant activity was seen in the IgG(2) class.


Comparison of antibodies against different viruses in cerebrospinal fluid and serum samples from patients with multiple sclerosis. The occurrence of a local production in the central nervous system (CNS) of antibodies against different selected viruses was analyzed by comparison of titers in serum and cerebrospinal fluid samples from groups of 50 patients with multiple sclerosis from Finland, Norway, and Sweden. Measles antibodies were determined in hemagglutination inhibition, hemolysis inhibition, and nucleocapsid complement fixation tests; mumps, parainfluenza virus type 1, and rubella virus antibodies were determined in hemagglutination inhibition tests; and herpes simplex virus type 1 antibodies were determined in passive hemagglutination tests. For reference purposes tests were also made for adenovirus antibodies in penton hemagglutination enhancement tests and poliovirus antibodies in neutralization enhancement tests. Among the 150 multiple sclerosis patients, a local production of antibodies against measles virus was found in the CNS in 57%, against rubella virus in 19%, mumps virus in 15%, herpes simplex virus type 1 in 11%, and parainfluenza virus type 1 (Sendai) in 3%. A local production in the CNS of antibodies against any of the viruses studied was found in 71% of multiple sclerosis patients. These included 48, 16, and 7% that produced antibodies to one, two, and three or more viruses, respectively.


Virus production and release, cell longevity, and cloning efficiency of chicken embryo fibroblasts infected with Rous sarcoma virus. Continuous virus production is a characteristic of chicken embryo fibroblasts (CEF) infected and transformed by a nondefective Schmidt-Ruppin subgroup A Rous sarcoma virus. This virus production has been examined with particular attention to the amount of newly budded virus which remained cell-associated, and to the amount and degree of viral aggregation at the cell surface and in the fluid tissue culture medium. The total biologically active virus associated with a Schmidt-Ruppin subgroup A Rous sarcoma virus-infected CEF culture was divided almost equally between that portion of virus which was present in the fluid medium and that portion which was cell-associated. Various mechanical and enzymatic methods were used to remove cell-bound virus and to disperse aggregates of virus in the tissue culture medium to assess cell production of virus per hour accurately, which was determined as an average of 16.4 focus-forming units per cell per hour. With appropriate culture conditions, it was found that Schmidt-Ruppin subgroup A Rous sarcoma virus-infected and -transformed CEF replicated faster, could be passaged more times, and grew to higher cell densities than did normal CEF and CEF infected with a subgroup A Rous associated virus. Subgroup A Rous sárcoma virus-infected CEF cloned with much lower efficiency than did subgroup A Rous associated virus-infected CEF or normal CEF. Experiments employing a temperature-sensitive mutant of subgroup A Schmidt-Ruppin Rous sarcoma virus- and Rous associated virus-infected CEF indicated that the poor cloning efficiency of Schmidt-Ruppin subgroup A Rous sarcoma virus infected cells was not due to the constant production of virus but was probably related to some property associated with transformation of the cell by Rous sarcoma virus.


Cell-mediated immunity in humans during viral infection: dermal hypersensitivity and in vitro lymphocyte proliferation during mild viral respiratory infections. In vitro lymphocyte proliferation in response to phytohemagglutinin and streptokinase-streptodornase and delayed dermal hypersensitivity to several antigens were assessed in patients with mild viral upper respiratory infections. The response to phytohemagglutinin in 18 patients was not diminished during the viral infection. Deoxyribonucleic acid synthesis induced by streptokinase-streptodornase remained unaffected by the viral illness in five patients, and skin test reactivity was not depressed during the viral infection. Thus, it appeared that localized viral upper respiratory infections were not associated with suppression of systemic cell-mediated immunity.


Effect of parainfluenza-3 virus neuraminidase on the structure of the gel phase of bovine nasal secretion. The gel phase of bovine nasal secretion was separated from the liquid phase and exposed to parainfluenza-3 virus or Vibrio cholerae neuraminidase under optimal in vitro conditions. N-Acetylneuraminic acid was released from the gel by either neuraminidase. The release of N-acetylneuraminic acid was accompanied by a decrease in the net negative charge of the gel, as evidenced by free zone electrophoresis. Scanning electron microscopy showed that after exposure to neuraminidases the regular network structure of the untreated gel attained a coarse, irregular structure. Samples of gel incubated with 0.15 M sodium chloride under similar conditions did not show changes in the net charge or morphology.


Pathogenesis of human parainfluenza type 3 virus infection in hamster tracheal organ culture. Hamster tracheal organ culture was employed as a model for the study of the pathogenesis of human parainfluenza type 3 virus infection. It clearly supported replication of the virus over a 2-week period of time. Infected tracheal explants were examined with light, electron, and immunofluorescence microscopy. They exhibited specific cytopathologic alterations including nuclear swelling and chromatin margination, multinucleated syncytia and binucleated epithelial cells, and fibroblasts and chondrocytes. Focal destruction and denudation of the respiratory epithelium occurred in later stages of infection. Virus was detected in close association with cilia and was observed budding off the unit membrane of epithelial cells.


Induction of disseminated virulent cytomegalovirus infection by immunosuppression of naturally chronically infected wild mice. Wild mice immunosuppressed with antithymocytic sera show a high incidence of disseminated virulent cytomegalovirus infection.


The mechanism of the adjuvant effect of Bordetella pertussis: the substance responsible for the selective enhancement of IgE antibody production.


Mast cell degranulation: a comparison of the inhibitory actions of disodium cromoglycate, drugs known to influence the level of intracellular cyclic nucleotide and diisopropylfluorophosphate (DFP).


Time course of homocytotropic and passive haemagglutinating antibody synthesis in the marsupial Setonix brachyurus (the quokka).


Arthropod-borne disease as a possible factor limiting the distribution of birds.


Radioassays for the proteolytic enzyme secreted by living eggs of Schistosoma mansoni.


Polyhydric alcohols: structure-supporting solvents for polypeptides and proteins.


Some considerations of after-care: problems of therapy, training and preceptoring.


Effects of x-radiation on growth and function of the repair blastema (granulation tissue). III. Measurements of pharmacodynamic activity in vitro.


An insoluble structural glycoprotein a major constituent of the zonula Zinni.


Effect of oxathiol on the uptake of absorbed doses from polonium-210 in the rat.


Antagonism of renal angiographic effects of cadmium by zinc.


Familial epilepsy and yellow teeth--a disease of the CNS associated with enamel hypoplasia.


Growth and body proportions in 54 boys and men with Klinefelter's syndrome.


On the binding of Schiff's reagent in the PAS reaction: effects of molecular configurations in models.


Organ culture of adult guinea-pig intestine. II. Biochemical and ultracytochemical findings.


Fine structure of the pars intermedia in the rhesus monkey, Macaca mulatta.


Fine structure, origin and fate of extracellular materials in the interspace between the presumptive lens and presumptive retina of the chick embryo.


Angiography in hepatic cysts and tumors in the rat.


Studies on energy and electron transfer systems in the green photosynthetic bacterium Chloropseudomonas ethylica strain 2-K. II. Composition of pigments and electron transfer systems.


The effect of transection of the glossopharyngeal nerve upon the structure, cholinesterase activity and innervation of taste buds in rabbits.


The inhibition mechanism of the cytochrome oxidase reaction. V. Quantitative analyses of the formation of the kinetically inactive complex of cytochrome oxidase with cyanide.


Adenylate kinase activity. In ciliary axonemes isolated from Tetrahymena pyriformis.


The isolation and characterization of glycopeptides from plasma membranes of an ascites hepatoma, AH 66.


Studies on triglyceride synthesis in lipid micelles from adipose tissue.


The role of direct phosphorylation in the mechanism of adenosine utilization for nucleotide synthesis in mouse erythrocytes.


Carboxypeptidase CN. II. Stability and some chemical and kinetic properties.


Inhibition of protein kinase and cyclic AMP phosphodiesterase by eritadenine isoamyl ester. A possible mechanism for its biphasic effects on cyclic AMP-dependent lipolysis, steroidogenesis and amino acid uptake.


Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase.


Studies of the insulin-like actions of polyamines on lipid and glucose metabolism in adipose tissue cells.


Cyclic adenosine 3':5'-monophosphate and cyclic guanosine 3':5'-monophosphate phosphodiesterase activities are under separate genetic control.


Cytidine 5'-monophosphosialic acid hydrolase. Subcellular location and properties.


Studies on receptor-mediated activation of adenylyl cyclases. I. Preparation and description of general properties of an adenylyl cyclase system in beef renal medullary membranes sensitive to neurohypophyseal hormones.


Studies on receptor-mediated activation of adenylyl cyclases. II. Nucleotide and nucleoside regulation of the activities of the beef renal medullary adenylyl cyclase and their stimulation by neurohypophyseal hormones.


Studies on receptor-mediated activation of adenylyl cyclases. III. Regulation by purine nucleotides of the activation of adenylyl cyclases from target organs for prostaglandins, luteinizing hormone, neurohypophyseal hormones and catecholamines. Tissue- and hormone-dependent variations.


Solubilization and purification of a prolactin receptor from the rabbit mammary gland.


Studies on receptor-mediated activation of adenylyl cyclases. V. Transient kinetics of the activation of beef renal medullary adenylyl cyclase by neurohypophyseal hormones: estimation of apparent rate constants of the receptor-hormone interaction.


Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. I. Purification and molecular properties.


Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. II. Selective chemical reactivity of amino and sulfhydryl groups.


Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. III. Isolation and sequences of peptides containing selectively labeled lysine and cysteine residues.


Physicochemical properties of the diphosphopyridine nucleotide-specific isocitrate dehydrogenase of pig heart.


Gonadotropin receptors in rat testes. Interaction of an ethanol-soluble testicular factor with human follicle-stimulating hormone and luteinizing hormone.


Some variables in the plaque assay of poliovirus.


Stability of DTP vaccine.

Stability of DTP vaccine.


Purification and some properties of formate dehydrogenase.


Cytochrome c-552 and cytochrome c-554 derived from Nitrosomonas europaea. Purification, properties, and their function in hydroxylamine oxidation.


Pyrophosphatidic acid. A new phospholipid from cryptococcus neoformans.


Effect of glucose and adenosine 3', 5'-cyclic monophosphate on the early development of Dictyostelium discoideum.


Studies on lipolysis in lipid micelles isolated from adipose tissue.


Studies on ESR spectra of chromatophores from Rhodospirillum rubrum.


Characterization of the external proteins of hamster fibroblasts. The results of metabolic labeling studies and enzymatic treatments followed by analysis on polyacrylamide gels show that the external proteins of hamster fibroblast cell lines, which have been identified by lactoperoxidase-catalyzed iodination, do not contain sulphated mucopolysaccharides or hyaluronic acid and are probably unrelated to collagen. Several of the iodinated species comigrate with carbohydrate-containing molecules. In particular, the major iodine-labeled polypeptide of normal fibroblasts appears to be a glycoprotein. This glycoprotein is absent or much reduced in virus-transformed cells, as detected both by iodination and by metabolic labeling. We conclude that the major iodinated polypeptide is not detected on transformed cells because it is absent rather than because it is masked. Approximate molecular weights of the external proteins are also reported.


Erythrophagocytosis by the epithelial cells of the bladder.


Influence of alkalinity in thin-layer chromatography systems for the separation of impurities in chlorpromazine, promazine and promethazine.


Pseudohypoaldosteronism: persistence of hyperaldosteronism and evidence for renal tubular and intestinal responsiveness to endogenous aldosterone.


The metabolism of vitamin D3 and 25-hydroxyvitamin D3 in normal and anephric humans.


Plasma 17-hydroxyprogesterone, 21-deoxycortisol and cortisol in congenital adrenal hyperplasia.


Severe osteopenia in young adults associated with Cushing's syndrome due to micronodular adrenal disease.


Embolization of a mediastinal parathyroid adenoma.


Receptor binding properties of 125I-hFSH prepared by enzymatic iodination.


Sleep EEG stages and plasma growth hormone concentration in states of endogenous and exogenous hypercortisolemia or ACTH elevation.


Profile of circulating vasoactive substances in hemorrhagic shock and their pharmacologic manipulation. (a) Hemorrhage in dogs (to 45-50 mm Hg) was associated with a 10-fold increase in plasma renin activity (PRA) which remained elevated throughout the time-course of shock including the irreversible (decompensation) stage. The presence of angiotensin II (AII) in arterial blood was demonstrated by the bloodbathed organ technique and confirmed by blockade with specific AII antagonists (cysteine(8)-AII or isoleucine(8)-AII). The contribution of AII to systemic peripheral resistance during hemorrhage shock in dogs was established by administering AII antagonists which immediately cause a further fall in blood pressure.(b) Plasma catecholamines (CA) steadily increased during hemorrhage and peaked during compensation (a 100-fold increase). The CA decreased progressively during decompensation.(c) Prostaglandin (PG) E-like material was observed in arterial blood for 15-60 min (after hemorrhage); the peak arterial concentration was 2.6 ng/ml blood. Indomethacin (i.v., before 80% of maximum bleedout): (i) confirmed the presence of PGE, (ii) increased blood pressure, and (iii) increased blood loss.(d) Thus: peripheral resistance during hemorrhagic shock seems temporally correlated with blood CA levels (and not PRA), and the renin-AII system contributes to the maintenance of vascular resistance and may markedly decrease perfusion of organs, such as kidney; the administration of the proper combination of specific antagonists of vasoconstrictor humoral substances may radically improve organ perfusion and could contribute to ultimate recovery from hemorrhagic shock.


Thyroid hormone action: in vitro characterization of solubilized nuclear receptors from rat liver and cultured GH1 cells. We previously reported that putative nuclear receptors for thyroid hormone can be demonstrated by incubation of hormone either with intact GH(1) cells, a rat pituitary tumor cell line, or with isolated GH(1) cell nuclei and rat liver nuclei in vitro. We characterized further the kinetics of triiodothyronine (T3) and thyroxine (T4) binding and the biochemical properties of the nuclear receptor after extraction to a soluble form with 0.4 M KCl. In vitro binding of [(125)I]T3 and [(125)I]T4 with GH(1) cell and rat liver nuclear extract was examined at 0 degrees C and 37 degrees C. Equilibrium was attained within 5 min at 37 degrees C and 2 h at 0 degrees C. The binding activity from GH(1) cells was stable for at least 1 h at 37 degrees C and 10 days at - 20 degrees C. Chromatography on a weak carboxylic acid column and inactivation by trypsin and Pronase, but not by DNase or RNase, suggested that the putative receptor was a nonhistone protein. The estimated equilibrium dissociation constants (K(d)) for hormone binding to the solubilized nuclear binding activity was 1.80 x 10(-10) M (T3) and 1.20 x 10(-9) M (T4) for GH(1) cells and 1.57 x 10(-10) M (T3) and 2.0 x 10(-9) M (T4) for rat liver. These K(d) values for T3 are virtually identical to those which we previously reported with isolated rat liver nuclei and GH(1) cell nuclei in vitro. The 10-fold greater affinity for T3 compared to T4 in the nuclear extract is also identical to that observed with intact GH(1) cells. In addition, the [(125)I]T3 and [(125)I]T4 high-affinity binding in the nuclear extract were inhibited by either nonradioactive T3 or T4, which suggests that the binding activity in nuclear extract was identical for T3 and T4. In contrast, the binding activity for T4 and T3 in GH(1) cell cytosol was markedly different from that observed with nuclear extract (K(d) values were 2.87 x 10(-10) M for T4 and 1.13 x 10(-9) M for T3). Our results indicate that nuclear receptors for T3 and T4 can be isolated in a soluble and stable form with no apparent change in hormonal affinity. This should allow elucidation of the mechanisms of thyroid hormone action at the molecular level.


Hereditary hemolytic anemia with human erythrocyte pyrimidine 5'-nucleotidase deficiency. A severe deficiency of a red cell pyrimidine 5'-nucleotidase was found to be associated with hereditary hemolytic anemia in four members of three kindreds. The syndrome was characterized by marked increases above normal in red cell basophilic stippling, total nucleotides, and GSH and by a fairly severe deficiency of ribosephosphate pyrophosphokinase (EC 2.7.6.1.). Patient erythrocytes uniquely contained large amounts of pyrimidine 5'-ribonucleotides. In earlier studies, these were erroneously considered to be adenosine phosphates, since all previous investigations of the nucleotides of human red cells and reticulocytes have shown 97% or more to contain adenine. Total nucleotides in patient cells were present in amounts 3-6 times greater than normal, and approximately 80% contained pyrimidine. The ultraviolet spectral curves of deproteinized red cell extracts exhibited a shift in maximum absorbance from the usual 256-257 nm to approximately 266-270 nm, and absorbance at 250, 270, 280, and 290 nm, expressed as a ratio of that at 260 nm, differed greatly from normal. The spectral characteristics of extracts provide the basis of a readily performed screening procedure, which does not require enzyme assay. The nucleotidase activity in deficient red cells assayed less than 14%, and usually less than 10%, of normal and much less in terms of reticulocyte-rich blood, where it was consistently found to be increased. The enzyme has a pH optimum of 7.5-8.0, is inhibited by EDTA, and does not utilize purine 5'-ribonucleotides or beta-glycerophosphate as substrates. While comparatively few family members have been available thus far for study, initial data are compatible with an autosomal, recessive mode of transmission of the deficiency. The pyrimidine 5'-ribonucleotides are presumably derived from RNA degradation and, not being diffusible, accumulate when the enzyme catalyzing their dephosphorylation is deficient. It is postulated that the prominent basophilic stippling results from retarded ribosomal RNA degradation secondary to accumulation of degradation products, namely pyrimidine 5'-ribonucleotides. Ribosephosphate pyrophosphokinase deficiency is considered to be an epiphenomenon. The mechanism responsible for increased red cell GSH is unknown.


Contribution of the fatty acids of three low density serum lipoproteins to bovine milk fat.


A light and electron microscopic study of the liver in case of erythrohepatic protoporphyria and in griseofulvin-induced porphyria in mice. The light- and electron-microscopic appearances of a liver biopsy from a case of erythrohepatic protoporphyria are compared with the appearances of the liver in griseofulvin-induced porphyria in mice. Crystals are present in hepatocytes and in the bile secretory pathways in each case. The nature and significance of these is discussed.


Hereditary bovine syndactyly. II. Hyperthermia.


A test of the salience hypothesis of dream recall.


Simulation of the effect of recruitment on loudness relationships in speech.


Electron spin resonance spectroscopy of single crystals of concanavalin A.


Reactions in glass ionomer cements: II. An infrared spectroscopic study.


Collagenolytic activity of human gingival crevice fluid.


Electron spin resonance spectroscopy of aqueous solutions of concanavalin A.


Sensitivity of human diploid embryonic lung cells for isolation of ECHO viruses from sewage.


Myxovirus parainfluenzae type 4 A growth in monkey-kidney tissue cultures and respiratory epithelium of experimentally infected hamsters.


Maintenance of latent herpetic infection: an apparent role for anti-viral IgG.


Modulation of polymorphonuclear leukocyte-mediated antibody-dependent cellular cytotoxicity.


Induced collagenolytic activity by electrical stimulation of embryonic fibroblasts in tissue culture.


Potassium dependent phosphatase in mitochondria of rat submandibular gland.


Relative insensitivity of spleen cells to interferon.


Effects of processing on the amine content of pork bellies.


Susceptibility tests of anaerobic bacteria: statistical and clinical considerations.


Cyclophosphamide-potentiated West Nile viral encephalitis: relative influence of cellular and humoral factors.


Photodecomposition of mirex on silica gel chromatoplates exposed to natural and artificial light.


A comparison of two methods of gentamicin assay: an enzymatic procedure and an agar diffusion technique.


Serologic response to varicella-zoster membrane antigens measured by direct immunofluorescence.


Cytosine arabinoside for localized herpes zoster in patients with cancer: failure in a controlled trial.


Implication of mycotoxins for human health.


Workshop on hospital-associated infections.


Influenza research in the Soviet Union--1974.


Letter: Severe combined nephrotoxicity of BL-P1654 and gentamicin.


A hepatocarcinoma with osseous metastases.


Lipoproteins in a nonrecirculating perfusate of rat liver. Rat livers were perfused in a nonrecirculating system for 30-40 min with Krebs-Ringer bicarbonate-0.1% glucose solution gassed with 95% O(2)-5% CO(2) at 37 degrees C at a flow rate of 3 ml/g/min. The livers appeared normal as judged by O(2) uptake, bile flow, transaminase release, and net protein output (2.5 mg/g/hr). The perfusate was concentrated by ultrafiltration using Amicon PM-10 or PM-30 membranes. The concentrated perfusate was subjected to sequential ultracentrifugation at solution densities of 1.006, 1.04, 1.06, and 1.21, and the top fractions were analyzed for protein and lipid. The net release of protein in the four density classes, suitably corrected, averaged 39, 10, 5, and 20 micro g/g/hr. The lipid composition of the perfusate lipoprotein fractions differed from that of serum mainly in the high percentage of free cholesterol, reflecting the lack of exposure to lecithin:cholesterol acyltransferase. When rat serum was fractionated in the same way, most of the lipoprotein in the d 1.006-1.06 range had a density greater than 1.04. It was concluded from these experiments that the liver secretes very low density lipoprotein (VLDL), high density lipoprotein (HDL), and a modified form of VLDL containing less lipid. Comparison of secretion rates and serum lipoprotein levels leads to the conclusion that the latter are largely determined by catabolic rates. When labeled amino acids were present, the perfusate HDL had a higher specific activity than VLDL. Addition of carrier whole serum did not alter recovery of labeled lipoproteins, but when these were isolated from Golgi membranes after a 40-min perfusion, more than twice as much label was recovered in HDL, suggesting the presence of precursors within the Golgi. The main advantages of the nonrecirculation perfusion technique are the avoidance of catabolic reactions, simplicity, and complete control over the composition of the perfusing medium.


Subcellular localization of hexadecanedioic acid activation in human liver. The activation of hexadecanedioic acid has been studied in subcellular fractions of human liver. The activation capacity in a total homogenate of human liver was found to be 0.5 micro mole/min/g wet wt of tissue, about 10% of that for palmitic acid. Hexadecanedioic acid was activated by the mitochondrial and microsomal fractions. The mitochondrial enzyme is probably localized outside the inner mitochondrial compartment. The subcellular distribution of the hexadecanedioic acid activation was almost identical with the distribution of palmitic acid activation. Hexadecanedioic and palmitic acids seemed to compete for the same enzyme.


Stimulation of erythroblast maturation in vitro by sphingolipids. A lipid factor previously isolated from leukocytes and found to stimulate basophilic erythroblast formation in an in vitro system of incubated rabbit bone marrow cells has been analyzed by thin-layer chromatography, gas-liquid chromatography, and gas-liquid chromatography-mass spectrometry. The biologically active components are sphingosine ceramides of tetracosanoic and dehydrotetracosanoic acids. Tests of a series of related ceramides show a high degree of structural specificity for the C(24)-V-acyl compounds with significant but markedly lower activity of the C(22) analog. Commercially available sphingomyelin shows activity comparable to that of the tetracosanoic acid ceramide. Sphingosine and tetracosanic acid supplied in equimolar amounts have negligible activity. The results, in the context of other findings, suggest a possible supportive role of plasma ceramides and sphingomyelins in red cell maturation.


Influx of cholesterol into plasma in rabbits with fasting hyperbetalipoproteinemia. New Zealand white rabbits exhibited as much as a threefold increase in plasma cholesterol but no change in hepatic cholesterol when fasted for 7-9 days. Agarose electrophoresis and ultracentrifugation of plasma samples showed that only low density lipoprotein increased during fasting. Fasting changed the composition of the low density lipoprotein by increasing the percentage of cholesterol and decreasing the percentage of triglyceride while protein and phospholipid remained the same. Rates of cholesterol secretion into plasma, measured by Triton WR 1339 injection, and rates of plasma cholesteryl ester synthesis, determined by [2-(14)C]mevalonate injection, were similar for fed and fasted rabbits. These findings suggest that fasting hypercholesterolemia in rabbits did not result from increased production of low density lipoproteins. Triton WR 1339 was shown to inhibit plasma cholesterol esterification in vitro.


Analysis of alpha-lipoprotein cholesterol in 50 microl of plasma. A micromethod for quantitation of alpha-lipoprotein cholesterol was devised for gas-liquid chromatography to minimize plasma sample size and facilitate lipid studies using capillary blood from children or small animals. alpha-Lipoprotein cholesterol was measured by gas-liquid chromatography in 20 micro l of the centrifuged supernate obtained after addition of 5 micro l of mixed heparin-manganese chloride solution 1:1 (v/v) to 50 micro l of plasma. Comparison of venous alpha-lipoprotein cholesterol measured by gas-liquid chromatography with venous alpha-lipoprotein cholesterol measured by conventional heparin-manganese precipitation and ferric chloride (colorimetric) cholesterol determination gave a correlation coefficient of 0.98 for 80 plasma samples. Capillary alpha-lipoprotein cholesterol and venous alpha-lipoprotein cholesterol were closely correlated in 31 patients (r = 0.97).


Reversible protein kinase activation of hormone-sensitive lipase from chicken adipose tissue. Hormone-sensitive lipase partially purified from adipose tissue of laying hens was markedly activated by cyclic AMP-dependent protein kinase. Activation was approximately 4-fold (ranging up to as great as 10-fold) compared with the much lower degree of activation obtained with analogous preparations from rat and human adipose tissues (59 and 86%, respectively). The partially purified preparations contained adequate endogenous protein kinase activity to effect complete activation with addition of cyclic AMP, ATP, and Mg(2+). Activation was blocked by protein kinase inhibitor (from rabbit skeletal muscle) but could be restored fully by addition of excess exogenous protein kinase (from bovine skeletal muscle). The fully activated lipase was slowly deactivated by dialysis at 4 degrees C and then rapidly and almost fully reactivated by addition of cyclic AMP and ATP-Mg(2+). Reactivation was blocked by protein kinase inhibitor. This deactivation-reactivation cycle was rapid at 23 degrees C with dialysis against charcoal and could be demonstrated repeatedly using a single preparation. The reversible deactivation of protein kinase-activated enzyme is presumed to reflect the action of a lipase phosphatase. Lipase prepared from tissue previously exposed to glucagon yielded a much smaller degree of activation than lipase prepared from tissue not exposed to the lipolytic hormone, indicating that the physiological hormone-induced activation is probably similar to or identical with the protein kinase activation demonstrated in the cell-free preparations. Under the conditions of assay used, the partially purified lipase fraction contained diglyceride, monoglyceride, and lipoprotein lipase activities. However, treatment with cyclic AMP-dependent protein kinase had virtually no effect on these lipase activities.


Human T lymphocyte "E" rosette function. I. A process modulated by intracellular cyclic AMP. The capacity of normal human T lymphocytes to form rosettes with sheep red blood cells can be inhibited by drugs or agents which induce elevations in intracellular levels of cyclic AMP. The effect is early in the presence of agents which elicit rapid elevations in intracellular cyclic AMP (isoproterenol, aminophylline) and occurs later in the presence of cholera toxin which induces a dalayed increase in endogenous cyclic AMP. Dibutyryl cyclic AMP is inhibitory, and the effects of dibutyryl cyclic AMP and the adenyl cyclase stimulators are potentiated by inhibition of phosphodiesterase. These data provide substantial evidence that elevation of intracellular cyclic AMP diminishes E rosette function of lymphocytes.


Immunopathogenicity and oncogenicity of murine leukemia viruses. I. Induction of immunologic disease and lymphoma in (BALB-c times NZB)F1 mice by Scripps leukemia virus. This report clearly demonstrates that a systemic lupus erythematosus (SLE)-like syndrome and lymphoma can be induced in immunologically normal (BALB/c x NZB)F(1) mice by infection of neonates with a murine leukemia virus (MuLV) (Scripps leukemia virus [SLV] 60A) isolated from NZB lymphoblasts. SLV 60A was titered in vitro (XC test) and administered to newborn and adult (BALB/c x NZB)F(1) mice over six log(10) dilutions. Propagation of MuLV in the newborn recipients was indicated by greatly elevated serum Mu gs-1 levels which were proportional to the dose of virus given. The SLE-like syndrome was characterized by antinuclear antibodies (ANA) and immune complex-type glomerulonephritis. ANA production was related to the dose of virus and reached the highest levels at 8-16 wk. The incidence of glomerulonephritis was also correlated with the dose of virus and reached nearly 50% in the animals given the highest virus dose. Both titers of ANA and incidence of glomerulonephritis were greater in females than in males, although the amounts of Mu gs-1 in sera of both sexes were equal. The incidence of direct Coombs' positivity was not significantly affected by inoculation of this virus. The incidence and time of onset of thymocytic lymphoma were linearly related to the amount of virus inoculated. High serum Mu gs-1 levels predicted lymphoma development and reflected increases in the amount of infectious virus in the spleen. No induction of tumors, autoimmunity, or high serum Mu gs-1 levels followed administration of SLV 60A to 6-wk old (BALB/c x NZB)F(1) mice or inactivated 60A or active AKR virus to newborns.


Distribution of fibroblast surface antigen: association with fibrillar structures of normal cells and loss upon viral transformation. The localization of a cell type-specific, soluble fibroblast surface antigen (SFA) was studied by immunofluorescence and by scanning electron microscopy of the same cells. The antigen had an uneven distribution forming streaks on chick embryo fibroblasts. It was localized to membrane processes and ridges, with a diameter of 50-200 nm. The processes extended from the periphery of the cells to the substratum or to other cells. Trypsin treatment completely removed detectable amounts of SFA. The antigen was detectable within 1 h after trypsin-treated cells were reseeded. The reappearance of SFA correlated with the restoration of membrane processes. Fibroblasts transformed by Rous sarcoma virus (RSV) showed loss of all or most SFA. When normal cells were transformed without subcultivation and trypsinization a fibrillar extracellular network of SFA remained under the transformed fibroblasts while the cells themselves were negative in immunofluorescence. When fibroblasts infected by RSV mutants were transferred to nonpermissive temperature for transformation new SFA was detected within 2 h. These data lead us to propose that loss of stabilizing and anchoring effect of SFA molecules in fibrillar cell surface structures may be critical in altered growth control and malignant transformation.


Stimulation by endocytosis of the secretion of collagenase and neutral proteinase from rabbit synovial fibroblasts. Rabbit synovial fibroblasts in monolayer culture secrete a specific collagenase and a neutral endopeptidase into their serum-free culture medium. The rate of secretion of these two enzymes is increased after the ingestion and storage of latex particles within the vacuolar system of the cells. The increased rates of secretion of the neutral enzymes are stable for over 2 wk in the absence of a further phagocytic bout. In constrast there is little change in the extracellular levels of two lysosomal hydrolases, cathepsin D and beta-glucuronidase. The increase in the secretory rates for the two neutral enzymes is related to the number of latex particles ingested by the cells, and increases of up to 12-fold over the nonphagocytosing cultures were observed. A variety of other materials including mycostatin particles and dextran sulfate also induced increases in the secretion of collagenase. These results are discussed in relation to the turnover of connective tissue matrix macromolecules.


Active transport of amino acids by membrane vesicles of Thiobacillus neapolitanus.


Identification of intracellular polysaccharide granules in thin sections of Nocardia asteroides.


DNA synthesis in resting African green monkey kidney cells infected with defective SV 40.


The properties of the Sendai virus ribonucleoproteins involved in genome transcription in infected cells.


Structural phosphoproteins associated with ten rhabdoviruses.


Induction of both thymidine and deoxycytidine kinase activity by herpes viruses.


Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity.


Cell interaction with poly(rI)-poly(rC) and interferon induction in chick and mouse cells.


A possible precursor containing RNA of a bovine enterovirus: the provirion 11.


Renaturation of inactivated interferons: requirement for reduction of a major component; lack of requirement for reduction of a minor component.


The iodination of bovine enterovirus particles.


Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ.


Cell fusion by HeLa cells persistently infected with haemadsorption type 2 virus.


Location of the origin and terminus of replication in polyoma virus DNA.


Absence of glycoproteins in poliovirus particles.


Interferon-sepharose: priming of L cells for induction of interferon by MM virus.


Enhanced production of human interferon by u.v. irradiated cells.


Focal necrotic hepatitis in mice as a biological marker for differentiation of Herpesvirus hominis type 1 and type 2.


Dependence of RNA replication on continuous protein synthesis in a temperature-sensitive mutant of foot-and-mouth disease virus.


Comparative production of paramyxoviruses by avian heart cells.


Demonstration of a receptor for IgG in Syrian hamster cells transformed with herpes simplex virus.


Immunofluorescent studies of RD-114 virus replication in cell culture.


Infectivity of mengovirus replicative form. Relationship to cellular transcription.


Replication of simian virus 40 in permissive cells: assembly of virus components.


Isolation of herpes simplex virus DNA from virus particles and infected cells by electrophoresis in polyacrylamide gels.


Sequential steps in attachment of human rhinovirus type 2 to HeLa cells.


Electron microscopic studies of visna virus ribonucleic acid.


Myelin proteins from different regions of the central nervous system.


Phospholipid metabolism in experimental allergic encephalomyelitis: activity of mitochondrial phospholipase A2 of rat brain towards specifically labelled 1,2-diacyl-, 1-alk-1'-enyl-2-acyl- and 1-alkyl-2-acyl-sn-glycero-3-phosphorylcholine.


In vitro Ca2+ dependent proteolysis in brain tissues: a possible source of error in transmitter metabolism studies.


Na+-K+-activated ATPase and K+-activated p-nitrophenylphosphatase activities: a comparison of their regional localizations in the C57 mouse brain.


Plasma renin and angiotensin II levels in subarachnoid haemorrhage.


Cyclic nucleotide hydrolysis: some possible natural regulators in retina and rod outer segments.


Labeled asialo-human chorionic gonadotropin as a liver-scanning agent.


Preoperative localization of an insulinoma by external scanning with 131I-toluidine blue.


Letter: Improving the 99mTc-phosphate bone scan.


Extreme dysplasia in renal epithelium of a young woman dying from hepatocarcinoma.


Effect of cellulose and cholesterol on blood and yolk lipids and reproductive efficiency of the hen.


Characterisation of spontaneous and induced epithelial and mesenchymal tumours by their enzyme patterns.


Origin of osmophilic inclusion bodies in type II pneumocytes.


Mesenchymal renal neoplasms of infancy revisited.


Calcified hepatoblastoma in a newborn.

Calcified hepatoblastoma in a newborn.


Cytological studies on the absorptive surfaces of cestodes. VIII. Phosphohydrolase activity and cation adsorption in the tegument brush border of Hymenolepis diminuta.


Critical evaluation of use of effective protein fractions in developing pharmacokinetic models for drug distribution.


Synthesis and biological evaluation of 2-amino-4-hydroxy-6-hydroxymethylpteridine pyrophosphate.


Release of norepinephrine and dopamine-beta-hydroxylase by nerve stimulation. II. Effects of papaverine.


Ontogenesis of adrenergic and cholinergic neuroeffector transmission in chick embryo heart.


Increased sensitivity of sinoatrial pace-maker to acetylcholine and to catecholamines at the onset of autonomic neuroeffector transmission in chick embryo heart.


Luteolysis in normal and prostaglandin F2 alpha-treated pseudopregnant rabbits.


The arterial migration of trophoblast in the uterus of the golden hamster, Mesocricetus auratus.


Growth hormone responses to hypoglycaemia in women with secondary amenorrhoea.


The stimulation of mouse ovarian follicular growth in vitro by dibutyryl cyclic adenosine 3':5' monophosphate.


Current guidelines for the use of antimicrobial agents.


Laboratory aids in the diagnosis of gynecologic infections--progress and pitfalls.


Letter: Post-coital estrogen.

Letter: Post-coital estrogen. The efficacy of Premarin as a postcoital contraceptive agent was eva luated in 194 women.  The patients were instructed to take 4 2.5 mg tablets 3 times a day for 5 days.  There were no pregnancies.  Menses began on the average of 1.1 days before the expected menstrual period, and was not altered by the treatment.  Should the treatment fail to prevent pregnancy, abortion is recommended if desired.


Cycloalkanones. 4. Antifertility activity.


Potential antifertility agents. 6. Synthesis and biological activities of optical isomers of 4 beta-(p-methozyphenyl)-2 beta-methylcyclohexane-alpha-carboxylic acid and related compounds. The optical isomers of the title compound were synthesized and the biological potencies of the two enantiomers were compared.  There was essentially no difference in their hypocholesterolemic activities, as had been predicted, and little or no difference between their uterotropic potencies.  The approximately equal uterotropic activities seen with the enantiomers is explained in terms of stereochemical requirements at the receptor level for an estrogenic response.  A working model of an estrogenic receptor is proposed.  An accompanying paper provides support for the proposed model.


Congenital limb anomalies:frequency and aetiological factors. Data from the Edinburgh Register of the Newborn (1964-68).


Potential antifertility agents. 7. Synthesis and biological activities of 2-,3-, and 6-alkyl-substituted 4-aryl-2-methylcyclohexanecarboxylic acids.


Haemoglobin H disease and beta-thalassaemia. Clinical haematological and electrophoretic studies in a family from South Lebanon.


Mitochondrial inclusions in fibroblast culture from a patient with beta-methylcrotonylglycinuria.


In vivo synthesis of RNA by vesicular stomatitis virus and its mutants.


Triplication of a unique genetic segment in a simian virus 40-like virus of human origin and evolution of new viral genomes.


Characterization of a rearrangement in viral DNA: mapping of the circular simian virus 40-like DNA containing a triplication of a specific one-third of the viral genome.


Binding of divalent copper ions to aspartic acid residue 52 in hen egg-white lysozyme.


An amber dna mutant of Escherichia coli K12 affecting DNA ligase.


Phosphorylation of Escherichia coli RNA polymerase by rabbit skeletal muscle protein kinase.


A new class of promoter mutations in the lactose operon of Escherichia coli.


Transfer RNA genes in mitochondrial DNA of grande (wild-type) yeast.


Oncogenicity of the R-35 rat mammary tumor virus.


Prevention of lower limb paralysis by neutralization of type-C RNA virus in wild mice.


Comparative pathology of cells transformed by herpes simplex virus type 1 or type 2.


Ultrastructural study of herpes simplex virus infection of mouse cells chronically producing Rauscher leukemia virus.


Protection of mice from malignant tumor implants by enucleated tumor cells.


Possible gene complementation in in vitro cell lines of Ehrlich ascites tumor established by cell fusion with sendai virus.


Detection of Epstein-Barr virus-determined intracellular antigens in unfixed cells with labeled antibodies.


Carcinoembryonic antigen and blood group substances in carcinomas of the stomach and colon.


Distribution of virus particles and mammary tumor virus antigens in mouse mammary tumors, transformed BALB-c mouse kidney cells, and GR ascites leukemia cells.


Histocompatibility genes (the H-2 complex) and susceptibility to mammary tumor virus in mice.


Relationship between the cellular and viral antigens of a BALB-c myeloma and murine leukemia virus.


Antibodies unrelated to Epstein-Barr virus in human sera which precipitate soluble components of cultured lymphoblasts.


Suppression of the avian sarcoma virus genome in hamster-transformed cells made resistant to 8-azaguanine.


Quantitative studies on the developing vascular system of rat hepatoma.


Inhibitory effect of polychlorinated biphenyls on liver tumorigenesis in rats treated with 3'-methyl-4-dimethylaminoazobenzene, n-2-fluorenylacetamide, and diethylnitrosamine.


Erythroid hypoplasia in cats inoculated with feline leukemia virus.


Bone lesions in cats with anemia induced by feline leukemia virus.


Hepatic carcinogenesis of aflatoxin M1 in rainbow trout (Salmo gairdneri) and its enchancement by cyclopropene fatty acids.


Carcinogenic effects of oral diethylstilbestrol on C3H male mice with and without the mammary tumor virus.


Further observations on the production of oncornaviruses by MJY-alpha cell line.


Effect of metabolic inhibitors on killing of tumor cells by antibody and complement.


Changes in growth control and growth requirements associated with neoplastic transformation in vitro.


Protein modification, metabolic controls, and their significance in transformation in eukaryotic cells.


Rhinovirus RNA polymerase: products and kinetics of appearance in human diploid cells. The kinetics of appearance of an RNA-dependent RNA polymerase activity obtained from human embryo lung cells infected with rhinovirus type 2 have been followed by analysis of the RNA synthesized by the polymerase preparation in vitro. Little single-stranded RNA was synthesized and the proportion of replicative intermediate to replicative form was over threefold greater than obtained in vivo. The polymerase activity in vivo declined in the presence of cycloheximide showing that continued protein synthesis was necessary to maintain RNA replication.


Nature of the scrapie agent: evidence against a viroid. The postulated viroid nature of scrapie agent was tested. Since the agent could not be recovered after a carefully controlled phenol extraction of infected mouse brains, it is suggested that this agent is not a viroid.


Chromatographic analyses of isoaccepting tRNAs from avian tumor viruses. Low-molecular-weight RNA from transforming viruses (Rous sarcoma virus-Rous-associated virus 1, Schmidt-Ruppin strain of Rous sarcoma virus, and sarcoma-B(77)), from nontransforming viruses (Rous-associated virus 1 and sarcoma-NTB(77)), and from chicken liver, chicken embryo fibroblast, and Rous sarcoma virus-Rous-associated virus 1-transformed chicken embryo fibroblast was isolated and purified. To determine if there are modified, qualitatively or quantitatively different isoaccepting species of tRNA in these avian sarcoma viruses as compared with the cell of virus origin, chicken embryo fibroblast or normal chicken liver, methionyl-, arginyl-, and lysyl-tRNA (with high amino acid acceptance activity), and aspartyl- and glutamyl-tRNA from viral-trans-formed cells (with low viral amino acid acceptance activity) were co-chromatographed on reversed phase-5 chromatography columns, and elution profiles were compared. Although in each case the elution profile between a particular viral and host cell tRNA differed quantitatively, there was no qualitative difference in the profiles of corresponding tRNAs from either transforming or nontransforming viruses examined. Minor quantitative differences in the elution profiles might be a reflection of the metabolic state of the cells, since all evidence points to acceptor activity being of host rather than viral origin. Since, with the exception of selective packaging of methionyl-tRNA (IV) species by both transforming and nontransforming viruses, no selectivity was found for isoacceptor species of other tRNAs, it seems that such preferential packaging of methionyl-tRNA (IV) species has no bearing on the event of viral transformation.


Effect of chemically modified 70S RNA from avian myeloblastosis virus (AMV) upon the activity of AMV DNA polymerase. Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intact and was inactive as a template for the AMV DNA polymerase. Furthermore, it inhibited the reaction catalyzed by AMV polymerase when 70S RNA was used as template-primer and had no effect on the two tested bacterial polymerases. Protection against the 70S (epsilonA) RNA inhibition was observed when 70S RNA was primed with oligo(dT) indicating preference of the polymerase for the oligo(dT) primed regions.


Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. An efficient and quantitative assay for infectious Rous sarcoma virus and reticuloendotheliosis virus DNAs is described. The specific infectivities of viral DNA corresponded to one infectious unit per 10(5) to 10(6) viral DNA molecules. Infection with viral DNA followed one-hit kinetics. The minimal size of infectious Rous sarcoma virus DNA was approximately 6 million daltons, whereas the minimal size of infectious reticuloendotheliosis virus DNA was larger, 10 to 20 million daltons.


Reticuloendotheliosis virus nucleic acid sequences in cellular DNA. Reticuloendotheliosis virus 60S RNA labeled with (125)I, or reticuloendotheliosis virus complementary DNA labeled with (3)H, were hybridized to DNAs from infected chicken and pheasant cells. Most of the sequences of the viral RNA were found in the infected cell DNAs. The reticuloendotheliosis viruses, therefore, replicate through a DNA intermediate. The same labeled nucleic acids were hybridized to DNA of uninfected chicken, pheasant, quail, turkey, and duck. About 10% of the sequences of reticuloendotheliosis virus RNA were present in the DNA of uninfected chicken, pheasant, quail, and turkey. None were detected in DNA of duck. The specificity of the hybridization was shown by competition between unlabeled and (125)I-labeled viral RNAs and by determination of melting temperatures. In contrast, (125)I-labeled RNA of Rous-associated virus-O, an avian leukosis-sarcoma virus, hybridized 55% to DNA of uninfected chicken, 20% to DNA of uninfected pheasant, 15% to DNA of uninfected quail, 10% to DNA of uninfected turkey, and less than 1% to DNA of uninfected duck.


Effect of interferon upon the primary and secondary transcription of vesicular stomatitis and influenza viruses. The synthesis of viral complementary RNA by vesicular stomatitis virus in permissive cells can be distinguished operationally into two phases, primary transcription, which can be observed in cycloheximide treated cells, and secondary transcription, which represents the further synthesis of viral complementary RNA obtained in untreated cells. Pretreatment of mouse L cells or chicken embryo fibroblasts with the homologous interferon and, for chicken embryo fibroblast cells, poly(rI):poly(rC), inhibits the production of infectious virus and reduces the synthesis of viral complementary RNA to levels comparable to that obtained during primary transcription. Treatment of mouse L cells with interferon plus cycloheximide also gives levels of vesicular stomatitis viral complementary RNA synthesis comparable to that observed with the cycloheximide treatment alone. Similar results have been obtained for interferontreated cells subsequently infected with influenza virus (strain WSN). The results are interpreted as indicating that inhibition of virus development by interferon does not act at the level of primary transcription but rather at an intermediate step between primary and secondary transcription, such as viral protein synthesis.