Reversion and induction of Rous sarcoma virus expression in virus-transformed baby hamster kidney cells.


Isolation and characterization of a large molecular-weight polypeptide of herpes simplex virus type 1.


Glycoprotein fragment associated with vesicular stomatitis virus after proteolytic digestion.


Entero-bacterial formation of cyclohexylamine in rats ingesting cyclamate.


Kinetic experiments on the synergistic effect of NADH on microsomal drug oxidation.


Commentary. EB virus, infectious mononucleosis, and cancer: the closing of the web.


Expression of Epstein-Barr viral capsid, complement fixing, and nuclear antigens in stationary and exponential phase cultures. Three continuous lymphoblastoid cell lines, 2 productive of nucleocapsids and 1 nonproductive line, were studied for their content of Epstein-Barr viral (EBV) antigens during transition from stationary to logarithmic phase growth. As a preliminary step, viable cells were separated from degenerating ones in discontinuous gradients of serum albumin. Viral capsid antigens were found in both living and dead cells of the 2 producer lines; however, complement fixing (CF) antigens and nuclear antigen were detected only in viable cell subpopulations. The content of antigen detectable in extracts of viable cells by complement fixation remained constant in replicating and resting cultures; further, all viable cells of the 3 lines demonstrated intranuclear antigen by anticomplement immunofluorescence in all stages of growth. In contrast, the proportion of cells with viral capsid antigen in the producer lines increased 7- to 24-fold following entry of resting populations into the phase of exponential growth.The results suggest that expression of viral capsid antigens is discontinuous and is initiated in response to events in log phase, possibly DNA synthesis or mitosis. Expression of the complement fixing and nuclear antigens in continuous in viable cells. These findings emphasize the intimate relationship of the CF and nuclear antigen to the transformed state and suggest that study of this antigen complex will shed light on the mechanisms of lymphocyte transformation by EBV.


Measurement of human plasma renin substrate concentration by angiotensin I radioimmunoassay: a discussion of methodical problems.


An evaluation of the diagnostic value of haptoglobin and transferrin levels in serum.


Growth hormone studies in patients with rheumatoid arthritis with or without glucocorticoid therapy.


Comparative investigation on the activity of a synthetic corticotrophin (18-ACTH). A clinical trial.


Reaction of methyl methanesulphonate with nucleic acids of fetal and newborn rats in vivo.


Comparative study of the phosphatase activity in the semen of bovines (Bos bubalis and Bos taurus) in Egypt.


Intracytoplasmic myocardial inclusions in chickens.


Isolation, cultivation and characterization of a poxvirus from some horses in Kenya.


Studies on bovine leukemia. I. Establishment of type C virus producing cell lines.


An improved in vitro bioassay method for measuring luteinizing hormone (LH) activity using mouse Leydig cell preparations.


Cyclic AMP in isolated corpora lutea of the rat: influence of gonadotrophins and prostaglandins.


Effect of pinealectomy on the 3H-5HT uptake of mast cells in the thyroid gland of the rat.


Pharmacological mediators in asthma.

Pharmacological mediators in asthma.


Progressive myoclonic epilepsy with Lafora's bodies. A clinico-pathological study.


Letter: 'Latent type IV' hyperlipoproteinemia.


Histochemical changes in the rat spinal cord after exposure to 6-aminonicotinamide.


Effects of chloroquine on neurons of long-term cultures of peripheral and central nervous system. A light and electron microscope study.


Morphological changes induced by ouabain in normal and neoplastic human glia in monolayer culture.


Insulin secretion in hyperlipoproteinemias.


Infantile neuroaxonal dystrophy or Seitelberger's disease. IV. Autonomic nervous system involvement: electron microscopic study in two siblings.


Genetics and physiology of colour vision. II.


Clinical aspects of neonatal hypoglycaemia.


Induction of SV40-tumour immunity by SV40-transformed cells in diffusion chambers.


Repression of hyaluronidase in Streptococcus mitis, ATCC 903.


The distribution of enzymes and bacteria in the small intestines of slaughter pigs.


Decreased oncogenic potential of SV40-transformed cells grown in diffusion chambers intraperitoneally.


The effect of lithium on blood pressure and on plasma renin substrate and renin in rats.


Pepstatin: lack of inhibition of the human renin-angiotensinogen reaction.


Effect of lithium on glucagon-stimulated cyclic AMP excretion in rats.


The relation of plasma renin activity to left ventricular hypertrophy and retinopathy in patients with arterial hypertension.


Lp(a) lipoprotein and pre-beta1-lipoprotein in young adults.


A theoretical treatment of the problem of virion attachment and infection of the host cell.


Metabolic effect of interferon preparations and some interferon inducers: activation of adenylate cyclase system.


Further evidences that priming is a non-antiviral function of interferon.


"Plexiform vermicellar arrays" in human cytomegalovirus-infected WI-38 human embryonic fibroblast cells.


The rehabilitation of addicts.

The rehabilitation of addicts.


The anion-binding functions of transferrin.


Calcium binding to elastin.

Calcium binding to elastin.


Cellular effects of lead.

Cellular effects of lead.


X-ray photoelectron spectroscopic studies of biological materials: metal ion protein binding and other analytical applications.


The child with acute leukemia.

The child with acute leukemia.


Development of a dust sampling system for hardrock mines based on gravimetric and quartz assessment.


X-ray diffraction determination of alpha-quartz in respirable and total dust samples from sand-blasting operations.


Relationship between exposure to asbestos, collagen formation, ferruginous bodies, and carcinoma.


Gastrointestinal malacoplakia. Report of a case and a review of the literature.


Comparative evaluation of a partial thromboplastin reagent containing a non-settling, particulate activator.


Immunoglobulin and autoantibody response in acute and chronic liver disease.


Reappraisal of the role of insulin in hypertriglyceridemia.


The hormones of the hypothalamus.

The hormones of the hypothalamus.


Hepatic abscess: changes in clinical, bacteriologic and therapeutic aspects.


Fasting hypoglycemia, hypercalcemia and hyperprolactinemia. Multiple endocrine neoplasia, type 1?


Parathyroid carcinoma in familial hyperparathyroidism.


Breast cancer in men. Report illustrating the value of endocrine ablation.


Adenoid cystic carcinoma of the cervix presenting as a primary bronchial neoplasm.


The "viruses of love" and cancer.

The "viruses of love" and cancer. A review of recent results of research into Herpesvirus is presented and a tentative association with cervical cancer is proposed.  2 types of the virus have been identified, HSV-1 which causes fever blisters or lip sores and HSV-2 which causes genital lesions.  The structure and composition of the virus is described.  In addition, mechanisms of productive and nonproductive infection at the cellular level are reviewed.  The pathogenesis of the HSV-1 and HSV-2 infections varies according to previous exposure, whether the patient is in a compromised medical condition and for newborns.  Many primary infections are subclinical, however when apparent are more severe than recurrent cases.  Most infections are localized to superficial areas of the skin or mucous membranes, which may be due to the virus' inability to spread through the connective tissue, variation in tissue susceptibility, proclivity of dissemination through blood or nerves and immune responses.  Primary oral infections range from inapparent to severe gingivostomatitis with extensive ulceration of the mouth, tongue and gum, fever, and adenopathy.  The occular conditions include conjunctivitis, cataracts, iridocyclitis, and panuveitis.  The genital infection occurs on the cervix and external genitalia in women and the penis, urethra, prostate and seminal vesicle in men.  Most cases of neonatal disease are caused by HSV-2.  The neurologic manifestations associated with the virus include encephalitis, meningitis and myelitis.  The recurrent disease consists of localized vesicular lesions caused by various stress factors.  It is suspected that for most recurrences the virus has persisted in the interim in a noninfectious form close to or at the site of the recurrency.  Data from several studies support this hypothesis.  Research into the mechanism of virus persistence and recurrence is focusing on the host cell and the role of immune factors.  Diagnosis is largely clinical although some laboratory aids have been useful.  Among the drugs used in prevention and therapy, the application of vital dye such as neutral red or proflavine, followed by exposure to light has been successful with recurrent lesions.  Research is warranted into the use of fever therapy and interferon.  Recently Hespesvirus has been associated with cancer in at least 6 cases.  Interpretation of data from animal as well as human studies is suggestive of a link but other explanatory factors have not been ruled out.


The use of a radioimmunoassay specific for human chorionic gonadotropin in patients with molar pregnancy and gestational trophoblastic disease.


Ultrastructure of sclerosing hemangioma. Specimens from 9 patients with the varying histopathologic patterns of sclerosing hemangioma were studied by electron microscopy. Not only were there numerous identifiable vascular spaces, but many of the cells had the ultrastructural characteristics of endothelial cells whether they were lining vascular spaces, forming organoid structures or were isolated. The Weibel-Palade bodies served as a marker for endothelial cells. They are rod-shaped, with a length up to 2 mu and a diameter up to .1 mu, and contain a tubular substructure, the diameter of the individual tubules being 150 [unk]. Our findings clearly support Gross and Wolbach's theory that this group of lesions is of endothelial origin.


Experimental cytomegalovirus ophthalmitis. Cytomegalovirus can produce a severe necrotizing chorioretinitis in patients on immunosuppressive therapy and infants born with congenital cytomegaloviral inclusion disease. To study the effect of cytomegalovirus on the eye, murine cytomegalovirus was injected into the eyes of nonimmunosuppressed Swiss CD-1 weanling mice. The eyes were then prepared for virus titer, as well as light and electron microscopy at variable periods after inoculation (1 to 28 days). From days 2 to 6, the hallmarks of cytomegalovirus infection, intranuclear and intracytoplasmic viral inclusions, were evident within cytomegalic cells. The major site of reaction was in the uveal tract, where necrosis and inflammation were prominent. Viral particles budding through the nuclear membranes into the perinuclear cisternae and vacuoles with viral particles could be seen in the cytoplasm of infected cells. In lesions older than 2 weeks, only a mild mixed inflammatory infiltrate and fibrosis were observed. Morphologic alterations unaccompanied by inflammation occurred in the outer sensory retina overlying infected retinal pigment epithelial cells. Multiple necrotic foci with inclusion-bearing cells in the liver indicated the systemic spread of virus from the eye. The titer of virus recovered from the eye peaked at day 4 and then declined to low levels, but infectious virus could still be isolated at day 28, even though viral particles were not seen morphologically at or after day 14. Many of the alterations seen in the model resemble those found in the human cytomegaloviral ophthalmitis.


Male advancement in prenatal hand development.


Carbohydrate metabolism of heat-acclimated hamsters. IV. Hormonal control.


Effects of parathyroidectomy and cyclic AMP on renal transport of phosphate, calcium, and magnesium.


Potassium deficiency in rats with hereditary diabetes insipidus.


Relation of fibrinogen secretion (synthesis) to ACTH dose.


ACTH stimulation of fibrinogen biosynthesis.


Membrane depolarization, cyclic AMP, and glycerol release by brown adipose tissue.


Catecholamine receptor function in depressed patients.


Cultural aspects of psychotherapy.

Cultural aspects of psychotherapy.


Artificial slow-flow carotid angiography with tumors of the glomus jugulare and of the carotid body.


The use of multiple radionuclide imaging to differentiate the focal intrahepatic lesion. Thirty-five patients with various focal intrahepatic lesions were evaluated with multiple radionuclides. Comparison of the blood pool scans with angiography and histology demonstrated an excellent correlation of these studies. Diagnostic accuracy was improved by separating the blood and pool scans into 3 categories. Each category suggested, at most, one further study to provide specific diagnostic information. Thus, by utilizing 3 radiopharmaceuticals, one can be more specific in preoperative diagnoses.


Islet cell tumors of the pancreas.

Islet cell tumors of the pancreas.


In vitro cultivation of Plasmodium falciparum at high parasitemia.


Correlation of circulating serum antibody to the histologic findings in breast cancer.


Determination of 5-phosphoribosyl 1-pyrophosphate in mouse liver.


Continuous pH-stat titration method for the assay of lipoprotein lipase activity in vitro.


Identification of fatty acids by GC-MS using polar siloxane liquid phases.


Persistent viremia after regression of primary virus-induced feline fibrosarcomas.


The effect of ketamine on regional cerebral blood flow in man.


Duration of immunity in recycled turkey breeder hens vaccinated with a single dose of live avian encephalomyelitis virus vaccine.


Clinical evaluation of the most frequently used analgetics: morphine, pethidine, pentazocine, fentanyl and noramidopyrine (pyralgin).


Early in utero infection of porcine embryos and fetuses with SMEDI (entero-) viruses: mortality, antibody development, and viral persistence.


Pathogenic properties of six bovine picornavirus isolates in mice.


Effects of cord factor on microsomal enzymes.


Immune response in persistent infection. Further studies on persistent respiratory infection in man with para-influenza type 3 virus.


A new approach to the study of four nondepolarizing relaxants in man.


Effect of angiotensin and ACTH on gastric secretion.


Diagnostic value of thyrotrophin-releasing hormone in pituitary and hypothalamic diseases. Assessment of thyrotrophin and prolactin secretion in 100 patients.


Hepatocellular carcinoma and partial deficiency of alpha-1 antitrypsin (MZ).


Emphysema, cirrhosis, and hepatoma with alpha-1 antitrypsin deficiency.


Letter: Parainfluenza virus type 3 isolated in a case of bloody diarrhea.


Digitonin as an uncoupler of the partial reactions of Na+, K+-ATPase.


Analysis of Na+, K+ and nucleotide interactions in terms of a heterotropic relaxation model for (Na+-K+)-ATPase.


Studies of the effects of 2H2O on Na+, K+-ATPase.


The relationship of the K+-activated phosphatase to the Na+,K+-ATPase.


The effects of Ca2+ on ATPase and phosphatase activities of erythrocyte membranes.


The effects of antibodies to Na+, K+-ATPase on the reactions catalyzed by the enzyme.


Na+, K+ exchange and Na+, Na+ exchange in the giant axon of the squid.


Action of methanethiol on Na+, K+-ATPase: implications for hepatic coma.


Mechanism of the effects of lipid phase transitions on the Na+, K+-ATPase, and the role of protein conformational changes.


Ouabain sensitivity of the Na+, K+-ATPase from rat neonatal and human fetal and adult heart.


Antibody levels to multiple viral antigens in systemic lupus erythematosus and some other connective tissue diseases.


Regulation of enzyme activity during differentiation in Dictyostelium discoideum.


Inclusion bodies of prokaryotes.

Inclusion bodies of prokaryotes.


Slow virus diseases.

Slow virus diseases.


Cyclic AMP in prokaryotes.

Cyclic AMP in prokaryotes.


Studies on photosystem I. Characteristics of "310 material" isolated from spinach chloroplasts.


Metabolism of vitamin D3 in the chick embryo.


Biosynthesis of myo-inositol in rat mammary gland. Isolation and properties of the enzymes.


Effect of trypsin, phospholipases, and membrane-impermeable reagents on the uptake of palmitic acid by isolated rat liver cells.


The effect of trypsin treatment on rabbit muscle phosphofructokinase.


A new ternary complex between horse liver alcohol dehydrogenase, NAD+, and acetone.


A two-step procedure for purification of papain from extract of papaya latex.


Distribution of liver plasma membrane 5' nucleotidase as indicated by its reaction with anti-plasma membrane serum.


Analysis of the ATP-induced conformational changes in sarcoplasmic reticulum.


The reversal of experimental porphyria and the prevention of induction of hepatic mixed-function oxidase by acetate.


Studies on a HCO3 minus-stimulated ATPase and carbonic anhydrase system in rat liver lysosomes.


In vivo changes of cellulose, trehalose and glycogen during differentiation of Dictyostelium discoideum.


Growth in children with 45,XO Turner's syndrome.


Plasma renin activity in acute poststreptococcal glomerulonephritis and the haemolytic-uraemic syndrome.


Dose-response basis for settling a quartz threshold limit value: a new, simple formula for calculating the "lifetime dose" of quartz.


Studies on some pathogenic strains of free-living amoebae isolated from lakes in Poland.


Clinical application of cytochrome a(plus a3) assay of mitochondria from liver specimens: an aid in determining metabolic tolerance of liver remnant for hepatic resection. Measurement of cytochrome a(+a(3)) contents in liver mitochondria was made on 52 biopsy specimens of patients with liver tumor. Patients having higher cytochrome a(+a(3)) contents in mitochondria from remaining liver than those of normal human liver mitochondria could survive well major liver resections whether or not associated with liver cirrhosis. However, patients with cytochrome a(+a(3)) contents less than 0.5 x 10(-10) moles per mg protein showed a high rate of postoperative complication (80%) and death (40%) in spite of minor operation. In routinely used liver function tests such as serum albumin, A/G ratio, SGOT, total bilirubin, prothrombin time, BSP and TTT, there were no significant differences between patients with cytochrome a(+a(3)) contents more than 0.5 x 10(-10) moles per mg protein and those less than 0.5. The results indicate that routine laboratory studies do not have much diagnostic value in estimation of a marked decrease of mitochondrial cytochrome a(+a(3)) contents. It is suggested that the measurements of cytochrome a(+a(3)) of the remnant liver should be done prior to a contemplated major resection.


Chemotherapy for adenocarcinoma and alveolar cell carcinoma of the lung.


The role of early open lung biopsy in the diagnosis and treatment of Pneumocystis carinii pneumonia.


Hydrocortisone suppression test for Cushing syndrome. Therapy with anticonvulsants.


Nitrogen metabolism and ultrastructure in Anabaena cylindrica. II. The effect of molybdenum and vanadium.


A study of sporophore development in the myxomycete Protophysarum phloiogenum.


Hyphal tips of wild-type and spreading colonial mutants of Neurospora crassa.


Quantitative studies by electron microscopy on the sex-difference and the change during the oestrous cycle in the mouse anterior pituitary.


Xerosialography.

Xerosialography.


Opsoclonus associated with an intracranial tumor. A clinicopathologic case report.


Pullularia corneal ulcer.

Pullularia corneal ulcer.


Fibrous histiocytomas of the nose and paranasal sinuses.


Granular cell myoblastoma of the trachea.


Regional odontodysplasia. A review and a report of two cases.


inositol phosphate phosphatases of microbiological origin. Some properties of the partially purified phosphatases of Aspergillus ficuum NRRL 3135.


The limbic system: an interface.

The limbic system: an interface.


A new method for propagation of HVJ (Sendai virus) in vitro: high sensitivity and productivity in spreading cultures of fragmented chorioallantoic membrane of chick embryos.


Chemical and immunological studies of liver microsomes from mouse mutants with ultrastructurally abnormal hepatic endoplasmic reticulum.


Anti-anabolic effects of adenosine 3':5'-cyclic monophosphate. Inhibition of protein synthesis. 1. Evidence is presented that cyclic AMP inhibits the incorporation of l-[4,5-(3)H]leucine into protein in a cell-free system from rat liver. This inhibition occurs after aminoacyl-tRNA formation. 2. Microsomal fractions, isolated after the incubation of postmitochondrial supernatant with cyclic AMP and ATP, show a diminished ability to synthesize protein. Both cyclic AMP and ATP are required for this effect. 3. A possible physiological role for the anti-anabolic action of cyclic AMP is discussed in terms of the control of gluconeogenesis.


The effects of acetylcolletotrichin on the mitochondrial respiratory chain. 1. Acetylcolletotrichin is a phytotoxic compound that has been isolated from the culture medium of the fungus Colletotrichum capsici (Grove et al., 1966). 2. With isolated liver and kidney mitochondria acetylcolletotrichin markedly inhibited the oxidation of succinate and those substrates with NAD-linked dehydrogenases, but did not inhibit the oxidation of ascorbate in the presence of tetramethyl-p-phenylenediamine. In this respect its action was similar to that of antimycin A. 3. Acetylcolletotrichin differed from antimycin in that, even at high concentrations which produced a maximal inhibitory effect, its action was partially reversed by uncoupling agents. Also acetylcolletotrichin had no detectable effect on the oxidative activity of blowfly flight-muscle mitochondria and was not very effective with heart mitochondria. 4. Acetylcolletotrichin inhibited the oxidative activity of liver mitochondria more markedly when respiration was stimulated by ADP together with phosphate and was less effective when respiration was stimulated by uncoupling agents. 5. There was an unusual interaction between the succinate oxidation system and the oxidation of glutamate together with malate. Thus, glutamate together with malate, even in the presence of rotenone, markedly decreased the effectiveness of acetylcolletotrichin in inhibiting succinate oxidation. 6. These effects were paralleled in the observed redox changes of cytochrome c. 7. The unusual behaviour of the cytochromes b in the presence of acetylcolletotrichin is described, and it is suggested tentatively that this inhibitor acts between cytochromes b with absorption maxima at 30 degrees C of approximately 560 and 565nm.


Nitrogen metabolism in the isolated perfused rat liver. Nitrogen balance, redox state and rates of proteolysis. Nitrogen balances were measured in isolated perfused rat livers in the presence and absence of nitrogen donors. In all instances the balance apparently was incomplete. The expression [alanine][alpha-oxoglutarate]/[pyruvate][glutamate] remained fairly constant under the metabolic conditions studied, indicating that it may be at near-equilibrium. The source of the extra nitrogen seems to be derived from increased hepatic proteolysis. The addition of a nitrogen donor to the perfusate arrested proteolysis, as did the addition of pyruvate. The free mitochondrial [NAD(+)]/[NADH] ratio, calculated from the glutamate dehydrogenase and beta-hydroxybutyrate dehydrogenase reactants, showed similar values and exhibited parallel changes under most metabolic situations studied. These results suggest that, under the reported experimental conditions, both dehydrogenases share a common mitochondrial NAD pool. Glutamate dehydrogenase plays an important role in hepatic nitrogen metabolism in vivo.


Phosphodiesterases in human tissues. I. Identification and separation of enzymes active on bis(p-nitrophenyl)phosphate.


Phosphodiesterases in human tissues. II. Decreased hydrolysis of synthetic substrate by tissues from patients with the Niemann-Pick syndrome.


Interferon production by strains of herpesvirus associated with Marek's disease.


Preparation of fowl pox vaccine on chicken-embryo-dermis cell culture.


UDP glucose-4 epimerase from Saccharomyces fragilis: interaction with sugar phosphates at an effector site.


Biochemical effects of diquat and paraquat. Disturbance of the control of corticosteroid synthesis in rat adrenal and subsequent effects on the control of liver glycogen utilization. 1. Administration of diquat (NN'-ethylene-2,2'-bipyridilium) or paraquat (NN'-dimethyl-4,4'-bipyridilium) prevents the normal depletion of liver glycogen in starved rats. 2. There is an increase in blood glucose, which returns to normal values after approx. 7h. 3. After administration of diquat or paraquat, plasma corticosteroids increase to very high concentrations and remain high for at least 24h, but plasma ACTH (adrenocorticotrophin) is only increased for 4h. 4. Adrenal cyclic AMP is considerably increased after administration of diquat and remains significantly higher than control values for at least 24h. 5. It is suggested that diquat and paraquat increase the response of the adrenal cortex to ACTH.


Factors influencing growth of Mycoplasma synoviae.


Role of superoxide radical in mitochondrial dehydrogenase reactions.


Insulin degradation in insulinoma: evidence for the occurrence of an inactive form of glutathione-insulin transhydrogenase and for the absence of insulin A and B chains degrading protease(s).


Maintenance of the energy charge in the presence of large decreases in the total adenylate pool of Escherichia coli and concurrent changes in glucose-6-p, fructose-p2 and glycogen synthesis.


The meta-cleavage of catechol by a thermophilic Bacillus species.


Protein kinase activities during maturation in Xenopus laevis oocytes.


Commentary. Insulin receptors, cell membranes and hormone action.


Effects of methadone on steroid biosynthesis in rat adrenocortical cells.


Hormone and adenosine 3', 5' cyclic monophosphate stimulated phosphorylation of human erythrocyte membranes.


Lack of correlation between cyclic AMP synthesis and free fatty acid release in brown fat of cold-adapted rats.


The occurrence of an inhibitor of lipid peroxidation in rat liver soluble fraction and its effect on microsomal drug oxidations.


Papaverine-induced changes in cultured human melanoma cells.


Acinomycin D and purine transport in cultured rat hepatoma cells.


Rabbit testicular capsular contractions--prostaglandins, cyclic adenosine 3',5'-monophosphate and epinephrine.


Inhibition by papaverine of cGMP and cAMP phosphodiesterases from the rat heart.


The role of basic substances of high molecular weight in the generalized Shwartzman reaction (GSR).


Apomorphine-induced accumulation of cyclic AMP in isolated retinas of the rabbit.


On the absorption, excretion and metabolism of pentaerythritoltetranicotinate.


The effect of glibenclamide on two enzymes important in gluconeogenesis.


The pituitary melanotrophic hormones.

The pituitary melanotrophic hormones.


Laryngeal paralysis due to peripheral nerve damage.


Histochemical and biochemical studies in ultraviolet irradiated tissue culture.


Action of niceritrol (pentaerythritoltetranicotinate) on lipid accumulation in aortas of cholesterol-fed rabbits independent of contemporary reduction of serum lipids.


Experiments with human papilloma virus in cell culture.


Differential effects of propranolol on heart rate and plasma renin activity in patients treated with minoxidil.


Cytochemical demonstration of dihydrofolate reductase activity.


The effect of niceritrol (pentaerythritoltetranicotinate) and clofibrate upon hyperlipemia and atherosclerosis induced in rabbits by cholesterol-free semisynthetic diets.


Calcium-binding protein and vitamin D metabolism in experimental protein malnutrition.


A comparative electron spin resonance study of the erythrocyte membrane in myotonic muscular dystrophy.


A predictable modification of enzyme specificity. Selective alteration of DNA bases by metal ions to promote cleavage specificity by deoxyribonuclease.


Purification and properties of a neutral protease from rat liver chromatin.


The separation, purification, and characterization of ethanolamine kinase and choline kinase from rat liver.


Molecular kinetics of beef heart lactate dehydrogenase.


A spin-label study of energy-coupled active transport in Escherichia coli membrane vesicles.


Purification and characterization of adenosine-adenosine cyclic 3',5'-monophosphate binding protein factors from rabbit erythrocytes.


Thermodynamic relationships between the mitochondrial oxidation-reduction reactions and cellular ATP levels in ascites tumor cells and perfused rat liver.


The kinetics of oxidation of reduced cytochrome c by ferricyanide derivatives.


Studies on polynucleotides containing hybrid sequences. Synthesis of oligonucleotides possessing a single ribonucleotidyl-(3'-5')-deoxyribonucleotide linkage.


Chloroacetaldehyde-modified dinucleoside phosphates. Dynamic fluorescence quenching and quenching due to intramolecular complexation.


Cylic adenosine 3',5'-monophosphate responses to concanavalin A in human lymphocytes. Evidence that the response involves specific carbohydrate receptors on the cell surface.


The mode of interaction of mitomycin C with deoxyribonucleic acid and other polynucleotides in vitro.


Inhibitory effect of complex formation with oligodeoxyribonucleotide ethyl phosphotriesters on transfer ribonucleic acid aminoacylation.


Lipid interactions in membranes of extremely halophilic bacteria. I. Electron spin resonance and dilatometric studies of bilayer structure.


Lipid interactions in membranes of extremely halophilic bacteria. II. Modification of the bilayer structure by squalene.


Studies on motional characteristics and distribution of protonated and anionic forms of spin-labeled 2,4-dinitrophenol in phospholipid bilayer membranes.


25-Hydroxyvitamin D3-1-hydroxylase. Inhibition in vitro by rat and pig tissues.


Interaction of glycyl-L-phenylalanine with Escherichia coli phenylalanyl-tRNA synthetase.


Inhibition of phosphodiesterases from Bacillus subtilis by nucleoside triphosphates.


The interaction of thrombin and heparin. Proflavine dye binding studies.


Biosynthesis of cholestanol: conversion of cholesterol into 4-cholesten-3-one by rat liver microsomes.


Formation of polyprenol monophosphate glucose in Shigella flexneri.


Diol lipids. XXVI. Identification of a threo-butane-2,3-diol phospholipid from Actinomyces olivaceus.


In vitro incorporation of (3H)serine into phospholipids of proliferating and calcifying epiphyseal cartilage and liver.


Changes in the phospholipid composition in the differentiating cellular slime mold, Dictyostelium discoideum.


Interaction by sonication of C-apolipoproteins with lipid: an electron microscopic study.


Preparative isoelectric focusing of human serum high-density lipoprotein (HDL3).


Lung-toxic furanoterpenoids produced by sweet potatoes (Ipomoea batatas) following microbial infection.


The action of protamine on clearing factor lipase and on plasma triglyceride metabolism.


Biosynthesis of diglyceride from phosphatidic acid in the membranes of Bacillus subtilis.


Composition and synthesis of gangliosides in mammary gland and milk of the bovine.


Theoretical analysis of the molecular motion of spin labels in membranes. ESR spectra of labeled Bacillus subtilis membranes.


Effect of ATP on the intermediary steps of the reaction of the (Na+ plusK+)-dependent enzyme system. III. Effect on the p-nitrophenylphosphatase activity of the system.


The photo-oxidation of horse heart cytochrome c and native cytochrome c2 by reaction centres from Rhodopseudomonas spheroides R26.


Flash photolysis-electron spin resonance study of the effect of o-phenanthroline and temperature on the decay time of the ESR signal B1 in reaction-center preparations and chromatophores of mutant and wild strains of Rhodopseudomonas spheroides and Rhodospirillum rubrum.


A new EPR-detectable component in the ubiquinol-cytochrome c reductase segment of the respiratory chain.


Separation of isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue.


Further characterization of a photosystem-II particle isolated from spinach chloroplasts by triton treatment: the reaction-center components.


An enzyme thermistor.

An enzyme thermistor.


Isolation of angiotensin-converting enzyme without kininase activity from hog and guinea pig plasma.


Conversion of UDP-D-glucuronic acid to UDP-D-apiose and UDP-D-xylose by an enzyme isolated from Lemna minor.


Pigeon liver diacetyl reductase. Kinetic and thermodynamic studies with NADH as coenzyme.


The nature of modifications by various anions of synthetic and hydrolytic activities of multifunctional glucose-6-phosphatase.


Molecular weight determinations of hydrophilic proteins by cationic detergent electrophoresis. Application to membrane proteins.


Non-specific protein binding by adsorbents designed for the specific affinity chromatography of sialidase in crude bacterial extracts.


The intermolecular cross-links in rat uterine collagen.


Pressure-induced elevation of phase transition temperature in dipalmitoylphosphatidylcholine bilayers. An electron spin resonance measurement of the enthalpy of phase transition.


Isolation of the Ochromanas danica plasma membrane and identification of several membrane enzymes.


The size of the repeating unit of the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast.


Preparation and properties of poly(5-ethylcytidylic acid), a poly(5-methylcytidylic acid) analogue.


Ribonuclease and phosphodiesterase activities in rat hepatocyte plasma membranes.


Multiple mechanisms of regulation of purine biosynthesis de novo in intact tumor cells.


Regulation of N-acetylglucosamine kinase synthesis in yeast.


A molecular orbital treatment of Cu(II) proteins.


Immunological stimulation with modified cancer cells.


Adrenocortical steroidogenesis and aminoglutethimide (A. G.). II. Cytochemical and ultrastructural changes. Pratical and theoretical values.


Antiviral effect of asparaginase and of Burkitt cell extracts in combination with other drugs.


Fractionation of cigarette smoke condensate prior to bioassays for carcinogenicity.


Developmental patterns of glycerokinase and cytochrome-c-oxidase in the brown adipose tissue of hypotrophic rats.


The effects of ACTH, dexamethasone and HCG on plasma levels of dihydrotestosterone in hirsutism.


Cytoplasmic tubule-containing vacuoles and endoplasmic tubuloreticular inclusions in the lymphocytes of a child with an unidentified form of cerebroretinal degeneration.


Effect of polyelectrolytes on the kinetics of ionic reactions. III. Hydrolysis of pyrophosphate in poly-(ethyleneimine) solutions.


Reagent gases for G.C.-M.S. analyses.

Reagent gases for G.C.-M.S. analyses.


Studies on isolation and characterization of ceramide trihexosidase.


Influence of storage temperature of florisil on analysis of polychlorinated biphenyls.


Toxicity study of two oil spill reagents toward Hudson River fish species.


Possible role of dark cells of the collecting tubules in acidification of the urine.


Biochemical changes induced by ethyl acetate in blood and liver of rat.


Letter: Camera or scanner for bone scans using 99Tcm pyrophosphate.


Multiple intraosseous lipomas with type-IV hyperlipoproteinemia. A case report.


Half-clearance time of 198Au colloid as a discriminant of liver abscess and malignancy.


Odontoma formation in the jaw following irradiation of an adolescent.


Angiographic evaluation of the regression of an extensive juvenile nasopharyngeal angiofibroma after radiation therapy: a case report with therapeutic implications.


Fibrous histiocytoma of the thumb.

Fibrous histiocytoma of the thumb.


Editorial: Late onset of puberty.

Editorial: Late onset of puberty.


Letter: Comparison between free thyroxine index and effective thyroxine ratio.


Plasma tryptophan and sheep.

Plasma tryptophan and sheep. Free, bound, and total plasma tryptophan (F.P.T., B.P.T., and T.P.T.) levels have been measured throughout the night in six young female volunteers. All-night polygraphic sleep recordings were also made. No direct temporal relationship was found between plasma tryptophan levels and specific sleep stages. The mean F.P.T. levels, however, were found to have a positive correlation with rapid-eye-movement (R.E.M.) sleep and a negative correlation with non-R.E.M. sleep. An inverse relationship existed between the F.P.T. and B.P.T. levels. There appeared to be a diurnal variation in F.P.T. levels, with high readings in the first half of the night.


Peripheral neuropathy in acromegaly.

Peripheral neuropathy in acromegaly.


Pathophysiology in multiple sclerosis.

Pathophysiology in multiple sclerosis.


The pattern of peripheral nerve regeneration induced by crush in rats with severe acrylamide neuropathy.


Clinical features of spinal vascular malformations.


Tangier disease.

Tangier disease.


Peripheral nerve changes in amyloid neuropathy.


Endoneurial space and its constituents in the sural nerve of patients with neuropathy.


Morphine and related compounds: evidence that they decrease available neurotransmitter in Aplysia californica.


Effects of electrical stimulation on acetylcholine levels in central cholinergic nerve terminals.


Synaptic connections between medullary inspiratory neurons and phrenic motoneurons as revealed by cross-correlation.


Mesencephalic dopamine neurons projecting to neocortex.


Histochemical confirmation for dopaminergic innervation of the rat cerebral cortex after destruction of the noradrenergic ascending pathways.


Choline uptake in glial cell cultures.

Choline uptake in glial cell cultures.


(3H)Uridine uptake and incorporation into RNA in the C-6 glial cells following dibutyryl cyclic AMP treatment.


L-Tryptophan as a selective histochemical marker for serotonergic neurons in single-cell recording studies.


Denervation in the primary olfactory pathway of mice: biochemical and morphological effects.


Simultaneous recording of pre- and postsynaptic potentials during the degeneration of the optic tract fiber input to the lateral geniculate nucleus of cats.


Reversible suppression of the geniculate PGO waves and of the concomitant increase of excitability of the intrageniculate optic nerve terminals in cats.


Hypophysectomy, ACTH1-10 and in vitro protein synthesis in rat brain stem slices.


6-Hydroxydopamine lesions of olfactory tubercles and caudate nuclei: effect on amphetamine-induced stereotyped behavior in rats.


Postmortem isolation of synaptosomes from human brain.


Tectal influences on cat ocular motoneurons.


Effects of trans-membrane polarization and TEA injection on monosynaptic actions from motor cortex, red nucleus and group Ia afferents on lumbar motoneurons in the monkey.


Characteristics of the rat C-6 glioma maintained in organ culture systems. Production of glial fibrillary acidic protein in the absence of gliofibrillogenesis.


Incorporation of sialic acid into sialidase-treated apolipoprotein of human, very low density lipoprotein by a pork liver sialytransferase.


Thermal inactivation of avian liver and muscle fructose 1,6-diphosphatases in the presence of fructose 1,6-diphosphate, AMP, and divalent cations.


Role of tocopherol in the reduction of mitochondrial NAD.


Some characteristics of the exchange of lecithin between rabbit erythrocytes and serum.


Enterovirus neutralizing activity in the gastrointestinal tract of piglets. Neutralizing activity against porcine enterovirus strain T80 was demonstrated in the contents of the stomach, duodenum or ileum of four piglets which were suckling dams whose milk contained neutralizing substances against the same virus. No neutralizing activity was detected in the gastrointestinal contents of an unsuckled piglet or in four weaned piglets. Extracts of intestinal tissue from each of the above piglets failed to neutralize the virus. Four weaned piglets were dosed orally with live T80 virus. From nine days after infection virus neutralizing activity was found in extracts of tissue prepared from the duodenum, jejunum, ileum, caecum and colon but not in the contents of the gastrointestinal tract and a serological response to the virus was demonstrated. No virus neutralizing activity was detected in gastrointestinal tissue or contents from four weaned piglets inoculated parenterally with live T80 virus or in four piglets dosed orally with inactivated T80 virus and these piglets did not respond serologically to the virus.


Sporulation and enterotoxin production by Clostridium perfringens type A under conditions of controlled pH and temperature.


Necrotic enteritis in broiler chickens. II. Pathology and proposed pathogenesis. The intestines from 124 dead, sick and normal broiler chickens from 24 cases of necrotic enteritis were subjected to histological examination. Tissue sections from the duodenum, jejunum, ileum and ceca from each broiler were examined histologically for lesions of necrotic enteritis and the presence of coccidia. Lesions of necrotic enteritis were present in one or more areas of the intestine in all but six of 94 dead or sick birds and they were most common and severe in the jejunum. Coccidia were found in only small numbers in both diseased and normal birds. Brown and Brenn stained sections showed Gram-positive bacilli intimately associated with early necrotic lesions on the tips of villi. Tissue sections from the intestines of sick birds permitted a proposed pathogenesis for this disease with the lesion starting at the tips of villi. The similarity in pathogenesis and pathological lesions in this disease of broilers and Clostridium perfringens type C enteritis in baby pigs is discussed.


Minimal inhibitory concentrations of twenty antimicrobial agents to animal pathogens. Minimal inhibitory concentration (M.I.C.) values as determined by an agar-plate-dilution method for 60 bacterial isolates, consisting of Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus of animal origin against 20 antimicrobial drugs are presented. Of all the drugs, gentamicin had the best in vitro antibacterial activity in terms of M.I.C. when considering all the species of organisms together, while spectinomycin had the least activity.An inoculum replicator was a convenient tool in carrying out the agar-plate-dilution method.A comparison of the M.I.C. values of 42 isolates of S. typhimurium with the results obtained by low level method and the Bauer-Kir-by method showed that with few exceptions, there is a general agreement.


Demonstration in Aspergillus niger of adenyl cyclase, a cyclic adenosine 3',5'-monophosphate-binding protein, and studies on intracellular and extracellular phosphodiesterases.


CO2 fixation by the facultative autotroph Thiobacillus novellus during autotrophy-heterotrophy interconversions.


Chronic benign congenital myopathy: fingerprint body type.


Regulation of chloramphenicol synthesis in Streptomyces sp. 3022a. Properties of arylamine synthetase, an enzyme involved in antibiotic biosynthesis.


Letter: Klippel-Trenaunay-Weber syndrome.


A review of acrylamide neurotoxicity. Part I. Properties, uses and human exposure.


A field trial to evaluate an intranasal infectious bovine rhinotracheitis vaccine.


Metabolic studies of 5-fluorouracil. II. Influence of the route of administration on the dynamics of distribution in man.


The prognostic significance of DHS skin tests in patients with carcinoma of bronchus.


Radiation carcinogenesis: an unusual familial occurrence of neoplasia following irradiation in childhood for benign disease.


Reticulum cell sarcoma of the colon following renal transplantation.


The management of Wilms' tumor: a comparison of two regimens.


Effect of blockade of angiotensin-I converting enzyme on the blood pressure of renal hypertensive rabbits.


Bacterial plasmids.

Bacterial plasmids.


Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing.


Increased collagen synthesis in Kirsten sarcoma virus-transformed BALB 3T3 cells grown in the presence of dibutyryl cyclic AMP.


Accumulation of water during transformation of cells by an avian sarcoma virus.


A new mechanism for steroid unresponsiveness: loss of nuclear binding activity of a steroid hormone receptor.


Enhancement by alkylating agents of chemical carcinogen transformation of hamster cells in culture.


A phosphorylation of steroids and a dienone-phenol rearrangement leading to a secosteroidal aldehyde which has a strong toxicity.


[Cytosoluble receptor of androgens in a striated skeletal muscle].


Fasting serum lipoproteins in rural Africans in Rhodesia, measured by membrane filtration and nephelometry.


Phospholipid interference and other variables in analysis of non-esterified fatty acids by copper soap formation.


Serum lipid and lipoprotein levels in normal Chinese.


Clinical application of cytochrome A assay of liver specimens for operative indication in critically ill patients with liver failure.


Young adult osteoporosis.

Young adult osteoporosis.


Studies on pathogenesis and treatment in postmenopausal and senile osteoporosis.


The physiology of parathyroid hormone.

The physiology of parathyroid hormone.


Hypocalcaemia and hypoparathyroidism.

Hypocalcaemia and hypoparathyroidism.


Hurler/Scheie genetic compound (mucopolysaccharidosis IH/IS) in Japanese brothers.


An extrarenal Wilms' tumor arising from a sacrococcygeal teratoma.


Subcutaneous mastectomy.

Subcutaneous mastectomy.


Proceedings. Urinary cyclic AMP excretion in thyroid disease.


Proceedings: Alterations in liver adenylate cyclase responsiveness during induction of tumours in rat liver with 3'-methyl-4-dimethylaminoazobenzene.


The inhibition of renin secretion by alpha-adrenergic stimulation in the isolated rat kidney.


The effects of exogenous parathyroid hormone on plasma and urinary adenosine 3', 5'-cyclic monophosphate in man.


Effects of vertebrate adipokinetic hormones on the rate of in vitro lipid release in insects.


Hormonal induction of threonine dehydratase in postnatal rat liver.


Lack of vitamin D3 synthesis by goldfish (Carassius auratus L.).


Circulating lipids in the golden hamster (Mesocricetus auratus).


Liver carabamyl phosphate synthetase in the primitive freshwater bony fishes (Chondrostei, Holostei).


Disc electrophoresis of plasma proteins of fish. Physical and chemical characters; localization of fibrinogen, transferrin and ceruloplasmin in the plasma of the rainbow trout (Salmo gairdnerii Richardson).


Effect of dibutyryl-cyclic AMP on collagen and non-collagen protein synthesis in cultured human cells.


Autoradiographic assessment of proteolytic enzyme action using skeletal connective tissue matrices.


Biochemical and morphological studies on collagens of horny sponges. Ircinia filaments compared to spongines.


Induction of liver growth by xenobiotic compounds and other stimuli.


The collagenase of carrageenin granuloma.


Experimental aspects of growth plate disorders. A study concerning chondrocytic lysosomes in different physical and metabolic conditions.


Infectious mononucleosis.

Infectious mononucleosis.


Dynamics of insulin release and microtubular-microfilamentous system. VI. Effect of D2O.


Location of feedback receptors: effects of intracranially implanted steroids on plasma LH and LRF response.


Synandrogenic and antiandrogenic effect of progestins: comparison with nonprogestational antiandrogens.


Estrogenic regulation of cytoplasmic receptor populations in estrogen-responsive tissues of the rat.


Actin in pancreatic islet cells.

Actin in pancreatic islet cells.


Possible role of cyclic AMP and prostaglandin E1 in the dopaminergic control of prolactin release.


Uterine responsiveness to estrogen in 4-day cycling and in estrogen-primed ovariectomized rats as assessed by uptake and incorporation of (3H) leucine.


Glucocorticoid-binding macromolecules in the lactating mammary gland of the vole.


Pituitary-adrenal function in lactating rats.


Effects of a single and prolonged ACTH treatment on adrenal, ovarian, uterine and renal blood flow in mice and golden hamsters.


Regulatory mechanism of muscle contraction with special reference to the Ca-troponin-tropomyosin system.


Phenylalanyl-tRNA synthetase from yeast. Steady-state kinetic investigation of the reaction mechanism.


Accessible DNA in chromatin.

Accessible DNA in chromatin.


Role of manganous ion in the kinetics of pig-heart NAD-specific isocitrate dehydrogenase.


Yeast inorganic pyrophosphatase: studies on metal binding.


Conformational changes in myosin under ATP hydrolysis.


Proteinase K from Tritirachium album Limber.


Enzymes involved in ketone utilization in different types of muscle: adaptation to exercise.


Phosphorylation of vinblastine-isolated microtubules from chick-embryonic muscles.


The role of acetyl-CoA in the reaction pathway of pig-liver pyruvate carboxylase.


Early biochemical changes in phytohemagglutinin-stimulated peripheral blood lymphocytes from normal and tumor-bearing rats.


Influence of iron deficiency on the cytochromes of Rhodopseudomonas spheroides.


A sex-dependent difference in the type of tumours induced by dimethylnitrosamine in the livers of C3Hf mice.


Ouabain-induced potentiation on the contractions of the guinea-pig vas deferens.


The effect of pH on tachyphylaxis to angiotensin peptides in the isolated guinea pig ileum and rat uterus.


The effect of metronidazole on striated muscle.


The role of muscarinic receptors in synaptic transmission and its modulation in the rabbit superior cervical ganglion.


The effect of prednisolone on the rat phrenic nerve-diaphragm preparation treated with hemicholinium.


Inhibition of oxytocic and hypotensive activities of bradykinin by bradykinin-binding antibodies.


Long-term cell culture of adult rat liver epithelial cells.


Virally transformed cells and cytochalasin B. I. The effect of cytochalasin B on cytokinesis, karyokinesis and DNA synthesis in cells.


Action of organophosphorus compounds on the p-nitrophenyl phosphatase of membranes from rabbit and guinea pig polymorphonuclear leucocytes.


The effects of cell population density on the plasma membrane of Acanthamoeba castellanii.


Isolation and characterization of norleucine-resistant baby hamster kidney cells.


Action of organophosphorus compounds on the cation-sensitive p-nitrophenyl phosphatase of membranes from the retinal nerve of the squid.


Inhibition by x-irradiation and antimetabolites of DNA synthesis without affecting cAMP elevation in isoproterenol-stimulated mouse parotid gland.


Synthesis of surface glycoproteins and glycosphingolipids in db-cAMP-treated normal and virus-transformed 3T3 cells.


Studies on the mechanism of actinomycin D resistance of an SV40-transformed hamster cell line.


Electronic coupling between motoneurones in the abducens nucleus of the cat.


A long propriospinal system with direct effect on motoneurones and on interneurones in the cat lumbosacral cord.


Inhibitory organization of the thalamic ventrobasal neurons with different peripheral representations.


Characteristics of the thalamic ventrobasal relay neurons as a function of conduction velocities of medial lemniscal fibers.


Manganese inhibits adenylate cyclase activity and stimulates phosphodiesterase activity in neuroblastoma cells: its possible implication in manganese-poisoning.


Early histochemical changes induced by manganese chloride in the renal tissue of rabbit.


Cell membrane permeability change: an important step in hormone action.


Effect of low calcium concentration on the oxidation of NAD-linked substrates in rat liver and tumor mitochondria.


Inhibition of Neisseria catarrhalis NE-11 transformation.


Restorative effect of cyclic AMP on the bioelectric processes of calcium deprived ganglia.


The effect of neuraminidase on the 5-hydroxytryptamine uptake of human platelets.


Inhibition of spontaneous and induced ovulation in rats by non-steroidal agents.


Oncornavirus released from long-term cultures of human leukemic cells.


On the modification of the divalent cation-binding by phospholipids in tumours.


The effect of theophylline on oxytocin induced contractions in the chronically catheterized pregnant rabbit.


Ultrastructure of the neurohypophysial glial cells following stalk transection in the rat.


In vitro and in vivo inhibitory action of 2-amino-4,6-dichloropyrimidine on polio and herpes virus.


How well does man thermoregulate during sleep?


Peripheral neuropathy associated with inhalation of methyl-n-butyl ketone.


Kininogenase in kidney after ligation of the ureter and after experimental aortic stenosis.


Rifampicin and cysteamine protect against the mushroom toxin phalloidin.


Unusual cases of facial paralysis.

Unusual cases of facial paralysis.


Liver phosphorylase phosphatase: regulation of activity by ATP and Mg ions.


Dissociation of rabbit muscle cyclic AMP-dependent protein kinase into catalytic and regulatory subunits by p-chloromercuribenzoate and methylmercuric chloride.


Eukaryotic cytochrome c-like properties of cytochrome c-550 (Thiobacillus novellus).


Absence of binding of thyroid-stimulating hormone subunits to specific sites of thyroid cells.


Activities of ribosomal cores deprived of proteins L7, L10, L11 and L12.


Alkaline phosphatase of Dictyostelium discoideum: cell surface location and colchicine effect on internalization during phagocytosis.


The use of a new series of cleavable protein-crosslinkers on the Escherichia coli ribosome.


Requirement for specific carbon sources in the low temperature induction of glycerol kinase in Neurospora crassa.


Experiments on the effectiveness of inactivatedd infectious bovine rhinotracheitis vaccine.


Immunization of cattle with apolyvalent bovine adenovirus vaccine.


Immunization of cattle with an attenuated IBR vaccine.


Vaccination of calves against infectious bovine rhinotracheitis using a temperature senstive mutant.


Renin, angiotensin, and norepinephrine in alloxan diabetes.


Estimation of specific antibody to type 2 Herpes simplex virus among patients with carcinoma of the uterine cervix.


Combined effect of nitrogen mustard and DNA repair inhibitor on the resistant tumor.


Cytogenetic and complementation analyses of recessive lethal mutations induced in the X chromosome of Drosophila by three alkylating agents.


The role of latent viruses in subline differentiation in inbred strains of mice.


Plasma androstenedione (A) and dihydrotesto-terone (DHT) in patients with trophoblastic disease and the effect of chemotherapy and oophorectomy.


Renal angiomyolipoma versus renal carcinoma. Is an angiographic differential diagnosis possible?


Dream recall and the menstrual cycle.

Dream recall and the menstrual cycle.


Hormone-mediated dimerization of microsomal estradiol receptor.


Conformational differences in Myosin, II. Evidence for differences in the conformation induced by bound or hydrolyzed adenosine triphosphate.


Single-copy nuclear DNA in the cytoplasm of undifferentiated embryo cells and tumor cells of man, chick and hamster.


Influence of noradrenalin, prostaglandin E1 and inhibitors of phosphodiesterase activity on levels of the cyclic adenosine 3':5'-monophosphate in somatic cell hybrids.


Specific estrogen binding sites on the liver chromatin of estrogen-pretreated roosters.


Biosynthesis of sphingomyelin. Transfer of phosphorylcholine from phosphatidylcholine to erythro-ceramide in a cell-free system.


Isolation and characterization of nuclear membranes from calf and rat thymus.


Aminopeptidase K from Tritirachium album Limber. I. Isolation and some properties.


Composition of mitochondrial lipids from rat adrenal glands.


The metabolism of sphingosine bases in Tetrahymena pyriformis. Sphingosine kinase and sphingosine-1-phosphate lyase.


On the mechanism of gluconeogenesis and its regulation. VIII. Differentiation of regulatory attacks of glucocorticoids, L-lysine and cyclic AMP in renal gluconeogenesis.


Preparation of pure choline and ethanolamine plasmalogens with the use of purified lipase from porcine pancreas.


Studies on the biosynthesis of cyclitols, XXX. Purification of myo-inositol-1-phosphate synthase of rat testes to homogeneity by affinity chromatography on NAD-sepharose.


Synthesis of labelled phosphatidyl-N,N-dimethylethanolamine and phosphatidylcholine.


Properties and specificities of sphingosine kinase from blood platelets.


Evidence for a hexosediphosphatase from the cytoplasm of spinach leaves.


A possible role of the glycerol phosphate cycle in cyclic AMP-stimulated gluconeogenesis from lactate in perfused rat livers.


Adenylate cyclase from Trypanosoma gambiense.


Purification and properties of a soluble cyclic AMP-dependent protein kinase from human platelets.


The relation between the blood-level of a substrate and enzyme kinetics studied with glycerol in the rat.


Inhibition of the tetrahydrofolic acid cycle in HeLa and LDR cells by parainfluenza virus 1 (Sendai).


The polymorphisms of red cell acid phosphatase, phosphoglucomutase, adenosine deaminase, and adenylate kinase in twins.


Studies on enzymes of renal gluconeogenesis in alcohol-fed rats.


Genetic markers and anthropometry in the populations of the Egyptian oases of El-Kharga and El-Dakhla.


An ultrastructural study of adenoid cystic carcinoma of the human salivary gland.


The effect of bacterial adjuvants on allograft survival after anti-lymphocyte serum (ALS) and donor bone marrow in mice.


Antibody response in rabbits after smallpox vaccination with varying tissue injury.


Changes in the level of hydrolytic enzymes in the caecum and intestine of rats infected with a virulent strain of Entamoeba histolytica intracaecally.


Experimental renal hypertension in rhesus monkeys: effect of surgical removal of ischaemic kidney on various parameters.


Hyper beta lipoproteinemia (familial hypercholesterolemia). A case report.


Antiviral activity of an ether-extracted nonviable preparation of Brucella abortus. Extraction of living Brucella abortus (strain 456) with aqueous ether yielded a nonviable, insoluble residue (Bru-Pel). When injected into mice, Bru-Pel was an effective, nontoxic interferon stimulus. Mice pretreated with Bru-Pel were protected against challenge with otherwise lethal doses of Semliki Forest virus. Significant protection was afforded when Bru-Pel was given as many as 7 days before virus challenge. Evidence is presented dealing with the complex nature of Bru-Pel and with the possibility that the antiviral activity of Bru-Pel may be associated not only with the production of interferon, but with a general increase in the level of nonspecific resistance in animals.


Resistance of female mice to vaginal infection induced by Herpesvirus hominis type 2: effects of immunization with Mycobacterium bovis, intravenous injection of specific Herpesvirus hominis type 2 antiserum, and a combination of these procedures. The susceptibility of 4- to 6-week-old female white Swiss mice to intravaginal inoculation with Herpesvirus hominis type 2 (HVH2) and the effect of prior intravenous immunization with Mycobacterium bovis (BCG) and/or treatment with specific HVH2 antiserum (SAS) were investigated. Mice inoculated intravaginally developed vaginitis, posterior paralysis, encephalitis, and death. Prior immunization with BCG either had no effect or appeared in some cases to enhance the course of the disease, whereas a single 0.5-ml intravenous injection of SAS provided significant protection. However, synergistic interaction of BCG immunization and treatment with SAS produced the greatest degree of protection in mice challenged intravaginally with HVH2.


Induction of interferon in mice by mycoplasmas. Interferon was induced in mice after intraperitoneal inoculation with four different mycoplasmas. Peak levels of between 100 and 300 U of interferon per ml were attained by 6 h postinfection with each of the mycoplasmas except Mycoplasma arthritidis, which induced higher titers (400 to 11,800 U/ml) by this time. A fifth mycoplasma, M. pulmonis, induced interferon inconsistently and at a later (72 to 96 h) time. Mycoplasmatales virus MVL51 and sterile mycoplasmal broth did not stimulate interferon production in vivo. All of the mycoplasmas and MVL51 failed to induce interferon in murine spleen cell, peritoneal exudate cell, or peripheral blood leukocyte cultures. Preinfecting the mycoplasmas with MVL51 or treating the organisms with trypsin or dilutions of specific antisera did not enhance their ability to induce interferon in vitro.


Agglutination of trypsinized sheep erythrocytes by the pili of Corynebacterium renale. Corynebacterium renale strains which possessed numerous pili agglutinated trypsinized sheep erythrocytes, whereas those possessing only a few pili did not show such activity. By electron microscopy, the pili of C. renale adhered to the ghost of trypsinized sheep erythrocytes. After the treatment of C. renale with the homologous anti-pili serum, neither the agglutination of trypsinized sheep erythrocytes nor the adherence to the ghost by the pili were found. The adhesive property of the pili of C. renale was thus demonstrated.


Hyporeactivity due to infection: recognition of a transferable hyporeactive factor in the serum of encephalomyocarditis virus-infected mice. As previously reported, mice infected with encephalomyocarditis (EMC) virus progressively develop a state of hyporeactivity to interferon induction as evidenced by an 80% reduction in serum interferon levels produced in response to four different inducers administered 96 h after initiation of infection. In the current report, this hyporesponsiveness was further documented in vitro in peritoneal exudate cells harvested at the time of maximal hyporeactivity (96 h) during EMC virus infection. These peritoneal cell preparations (containing no detectable interferon or virus) produced significantly less interferon than cells from uninfected mice following exposure to Newcastle disease virus or Chikungunya virus. More importantly, hyporeactivity could be transferred to normal peritoneal cells incubated in serum (containing no detectable interferon or virus) from EMC virus-infected mice during the hyporeactive period. Incubation of peritoneal exudate cells with up to a 1:200 dilution of serum from hyporeactive animals still resulted in a significant reduction in the interferon response induced by Newcastle disease virus or Chikungunya virus. Furthermore, it could be shown that this serum hyporeactive factor was effective in mouse cells but not in rabbit, chicken, or human cells.


Inability to detect hepatitis B virus or specific antigens in transformed chimpanzee lymphocytes. Transformed chimpanzee lymphocytes were examined to determine whether they would support the replication of hepatitis B virus. After 5 months, no hepatitis B virus, hepatitis B surface antigen, or antibody to hepatitis B surface antigen was detected.


In vitro tritium labeling of anti-sendai virus antibody. Anti-Sendai virus immunoglobulin G was tritium labeled in vitro by the borohydride exchange method. Tritium was incorporated into the antibody at 28.5 muCi per mg of protein, with little detectable loss of antiviral activity.


A decade of dreams: a review.

A decade of dreams: a review.


The accurate measurement of vanadium in airborne particulates.


Radiation damage in L-histidine-HCl monohydrate.


Enhancement of the radioprotective effects of 2-mercaptoethylamine on colony-forming cells by agents which alter cyclic nucleotide levels.


Toxicity to rabbit retina of "epithermal" neutron beam.


The effect of lithium carbonate on the sleep of depressed patients.


Androgen receptors in the nonhistone protein fractions of prostatic chromatin.


Papovaviridae.

Papovaviridae.


Comparison of the capsid polypeptides of various bluetongue virus serotypes.


Inhibition of gross murine leukemia virus replication by rifamycin SV and certain of its derivatives in vitro.


Antiviral antibiotics: inhibition of focus-formation in human or mouse cell cultures by sarcoma-inducing oncornaviruses with rifamycins.


Complexity of immunoreactive forms of peptide hormones in plasma and in tissue extracts.


Heterogeneity of circulating insulin and proinsulin in man.


Plasma proinsulin-like components.

Plasma proinsulin-like components.


Common mechanism of action of somatomedin and insulin on fat cells. Further evidence.


Characterization of preparations of radioiodinated human chorionic gonadotropin.


Neurohypophyseal hormones as precursors of hypophysiotropic hormones.


Polypeptide hormone-receptor interactions. A new approach to the study of pancreatic and gut glucagons.


Evaluation of anti-tumor effect of corticosteroid on human glioma in vitro.


Observations on the maturational appearance of the reproductive organs of male infants after treatment with ACTH and corticosteroids.


Histochemical demonstration of changes in liver cell enzymes in pregnant mice infected with Coxsackie B3 virus.


Intense phosphatase activity in the developing rete testis of the newborn rat.


Vaccination for reproductive efficiency in cattle.


Acidolin: an antibiotic produced by Lactobacillus acidophilus.


Causes and control of swine stillbirths.


A new medium for the detection and enumeration of Clostridium perfringens in foods.


Histamine releasers and hypoxic vasoconstriction in isolated cat lungs.


Adrenal adaptation to chronic exercise.

Adrenal adaptation to chronic exercise.


Central neural respiratory stimulatory effect of active respiration.


Periodic breathing during sleep.

Periodic breathing during sleep.


Eccrine sweat gland in Macaca mulatta: physiology, histochemistry, and distribution.


Primary carcinoma liver (a clinico pathological study of 185 cases with liver biopsies).


Cellular location of degradative enzymes in Staphylococcus aureus. Staphylococus aureus, ATCC 6538P, was fractionated into protoplast membranes, mesosomal vesicles, periplasm, and cytoplasm. These fractions and the culture fluid were then assayed for various degradative enzyme activities. They were not restricted to a single fraction nor dispersed homogeneously, but were distributed predominantly (on the basis of specific activity) as follows: nuclease in the culture fluid; alkaline phosphatase, 5'-nucleotidase, and acid phosphatase in the periplasm; adenosine triphosphatase in the protoplast membrane; and protease (low levels) in mesosomal vesicles. No significant esterase nor cell wall hydrolytic activity was found in any fraction. S. aureus 80/81 was studied for penicillinase activity after induction with benzyl penicillin; this enzyme was localized in the mesosomal vesicles. Electron microscopy did not reveal any ultrastructural changes associated with secretion of the extracellular fraction. Overall, these studies demonstrate that degradative enzymes are located in several surface compartments and that, therefore, the mesosome does not function as a prototype lysosome in S. aureus.


Regulation of serine transhydroxymethylase activity in Salmonella typhimurium. The regulation of serine transhydroxymethylase (EC 2.1.2.1.; l-serine:tetrahydrofolic-5,10-hydroxymethyltransferase) has been investigated in Salmonella typhimurium LT2. Our results indicate that limitation of a methionine auxotroph for methionine does not cause derepression of this enzyme as reported for Escherichia coli. However, a sixfold decrease in specific activity was observed when S. typhimurium cells were grown in glucose minimal medium supplemented with serine, glycine, methionine, adenine, guanine, and thymine. None of these compounds added to the growth medium individually produced more than a 42% reduction of wild-type enzyme activity. This enhanced repression by the combination of compounds suggests a form of cumulative repression of this enzyme. Growth of serine and thymine auxotrophs, with the respective requirement of each limiting, did not result in increased enzyme activity. However, growth of a purine auxotroph with a limiting amount of either guanine or inosine resulted in a five- to sevenfold increase in enzyme activity. A second condition causing significant derepression (fourfold increase) above the levels observed with cells grown in minimal medium was the addition of 0.5 mug of trimethoprim per ml, an inhibitor of the dihydrofolate reductase activity. (A partial report on this work was presented at 1974 meeting of the American Society for Microbiology.)


Mutants of Salmonella typhimurium defective in transport of branched-chain amino acids. Five mutants, CD15, CD31, CE4, CE5, and CE14, defective in the transport of branched-chain amino acids were isolated as glycyl-l-isoleucine and glycyl-l-valine requirers from an isoleucine-valine-requiring mutant, KA931, of Salmonella typhimurium LT2. Although these transport mutants do not grow in minimal medium supplemented with isoleucine (10 mug/ml) and valine (20 mug/ml), where the parent strain, KA931, grows normally, they do when the supplements are increased 10-fold in concentration, and the growth rate becomes comparable to that of the parent strain. When the apparent K(m) and V(max) for uptake of isoleucine, valine, and leucine in the transport mutants were compared to those of KA931, the K(m) was generally lower in the mutants, and the V(max) for isoleucine and leucine decreased to one-fourth to one-seventh and for valine one-eighth to one-fifteenth of that in the parent. In all mutants except CE5, the transport of methionine, arginine, threonine, and glycine was normal. The transport of threonine in CE5 appeared to be slightly impaired. In addition to the original mutation in the ilvC locus, the transport mutant has a mutation at the ilvT locus, which is closely linked to proC and may be located on the side of proC proximal to purE. About 26-fold difference in values of the co-transduction is noted in reciprocal transductions between KA502 and CD15 strains.


Properties of two homologous alkaline proteases from Streptomyces rectus. Some physicochemical properties of two thermostable proteases from Streptomyces rectus are described. The enzymes were judged to be identical with respect to molecular weight, inactivation with serine protease inhibitors, and in primary structure by peptide analysis. Amino acid analysis indicated the enzymes had identical compositions except for their amide content. The molecular weights of the enzymes were judged to be 28,000 by sedimentation equilibrium, 26,200 by sedimentation diffusion, and 29,100 from amino acid analysis. Titration of the proteases with diisopropylfluorophosphate and phenylmethane sulfonylfuoride indicate equivalent weights of 28,500 and 32,800 g, respectively, for the proteins. The pentapeptide around the serine residue reacting with diisopropylfluorophosphate was isolated and had the composition: Asx(1), Gly(1), Thr(1), Ser(1), Met(1).


Rhythms of enzyme activity associated with circadian conidiation in Neurospora crassa. The mycelial growth front of the band strain of Neurospora grown on a solid surface exhibits a circadian rhythm of conidiation. Enzyme assays on extracts from that mycelium have shown that the activities of 6 of 13 enzymes (nicotinamide adenine dinucleotide nucleosidase, isocitrate lyase, citrate synthase, glyceraldehydephosphate dehydrogenase, phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase) and soluble-protein content oscillate with the visible morphological change. The rhythmic enzymes associated with the Krebs and glyoxylate cycles are more active during conidiogenesis, whereas the activities of the rhythmic enzymes of glycolysis and the hexose monophosphate shunt are reduced during that phase. The absence of enzyme oscillations in wild-type and fluffy strains which do not form conidia under the conditions employed suggests that the enzyme fluctuations are associated with conidiogenesis itself. Oscillations of enzyme activity as a function of time are restricted to the growth front. A permanent record of rhythmicity associated with conidial and nonconidial regions does, however, exist in the mycelial mat behind the growth front. The activities of three enzymes (nicotinamide adenine dinucleotide nucleosidase, glucose-6-phosphate dehydrogenase, and phosphogluconate dehydrogenase) are not directly influenced by CO(2) concentration, but are correlated with the prescence or absence of conidiation which is controlled by CO(2) concentration. In contrast, citrate synthase and malate dehydrogenase activities are correlated with changes in CO(2) concentration.


Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression. In Escherichia coli, the synthesis of the flagellar organelle is sensitive to catabolite repression. Synthesis requires the presence of the cyclic adenosine monophosphate receptor protein (Crp) and 3',5'-cyclic adenosine monophosphate (cAMP); i.e., mutants that lack Crp or adenylcyclase (Cya) synthesize no flagella. We isolated and characterized a series of mutants (cfs) that restored flagella-forming ability in a Crp strain of E. coli. The mutations in these strains were transferred onto episomes and they were then introduced into a variety of other strains. The presence of the mutation resulted in flagella synthesis in Cya and Crp strains as well as in the wild type grown under conditions of catabolite repression. Deletion analysis and other genetic studies indicated that: (i) the cfs mutations had a dominant effect when they were in the transconfiguration in merodiploids: (ii) they occurred in or very close to the flaI gene: and (iii) their expression required the presence of an intact flaI gene adjacent to the cfs mutation. Biochemical studies showed that the synthesis of at least two flagellar polypeptides, the hook subunit and an amber fragment of flagellin, were absent in strains that carried a cya mutation. Their synthesis was depressed in strains grown under conditions of catabolite repression. The presence of the cfs mutation restored the specific synthesis of these two polypeptides. We suggest that the formation of the flaI gene product is the step in flagellar synthesis that is catabolite sensitive and requires cAMP. We propose a regulatory function for the product of the flaI gene.


Fractionation of two diguanylic acids with different internucleotide linkages and its application for analyses of the synthetic products of ribonuclease T1 and spontaneous reaction.


Salmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk). Mutants of Salmonella typhimurium defective in cytidine 5'-monophosphate (CMP) kinase (cmk) have been isolated. The mutants also lack the ability to phosphorylate 2'-deoxyCMP, indicating that one enzyme is responsible for the phosphorylation of both CMP and deoxyCMP to the corresponding diphosphates. In glucose minimal medium the mutants grow at the same rate as the parental strain; however, they excrete large quantities of pyrimidines into the growth medium. Cytidine but not deoxycytidine has been identified among the excreted products. The mutant phenotype suggests that the physiological role of CMP kinase is that of rephosphorylating CMP arising from the breakdown of messenger ribonucleic acid. This proposed role of CMP kinase is supported by the fact that a cmk(-) mutant is much more sensitive to any partial impairment of cytidine 5'-triphosphate synthetase than is the cmk(+) parent strain. The gene cmk has been located on the Salmonella chromosome at 38.5 min. No markers which can be cotransduced with cmk by phage P22 have been found.


Electron spin resonance study of the interaction between heavy meromyosin and Mn2 plus.


Energization of NTP-NDP kinase by acid-base transition leading to ATP formation.


Phosphorylation of endogenous hepatic proteins by adenosine 3',5'-monophosphate-dependent protein kinase.


Phosphodiesterase-phosphomonoesterases from Fusarium moniliforme. V. Mode of action on various nucleotides.


Studies on mitochondrial and microsomal nucleoside diphosphatases of rat liver.


Biochemical and topographical studies on the cell surface of Escherichia coli. I. Isolation and characterization of detergent-sensitive mutants.


Kinetic studies on the inhibition of nucleoside diphosphate kinase by desdanine.


Pyruvate kinase of Escherichia coli. Its role in supplying nucleoside triphosphates in cells under anaerobic conditions.


Interactions between elongation factor tu-guanosine triphosphate and ribosomes and the role of ribosome-bound transfer RNA in guanosine triphosphatase reaction.


Conversion of glucose and galactose to lipids by normal and phytohemagglutinin-stimulated lymphocytes.


Positional specificity of sn-glycerol 3-phosphate acylation during phosphatidate formation by rat liver microsomes.


Structural studies on anti-inflammatory polysaccharides from Streptomyces fradiae.


In vitro models for photosynthetic energy conversion.


Adenylate cyclase activity in fibroblasts transformed by Kirsten or Moloney sarcoma viruses. Decreased activity and loss of response to prostaglandin E1.


Characterization of the 4 S and 5 S forms of the estradiol receptor protein and their interaction with deoxyribonucleic acid.


On the specificity of the binding of the estradiol receptor protein to deoxyribonucleic acid.


Structural requirements for recognition of Escherichia coli initiator and non-initiator transfer ribonucleic acids by bacterial T factor.


Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. I. Evidence for an ecto-adenosine monophosphatase, adenosine triphosphatase, and -p-nitrophenyl phosphates.


Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. II. Properties and suitability as markers for the plasma membrane.


Purification and characterization of an inhibitor of elongation factor G-dependent guanosine triphosphatase reaction of ribosomes from ribosome wash of Escherichia coli Q13.


Rapid reciprocal changes in rat hepatic glycolytic enzyme and fructose diphosphatase activities following insulin and glucagon injection.


Rapid reciprocal changes in rat tissue enzyme activities following epinephrine injection.


Total reconstitution of copper-zinc superoxide dismutase.


Structure of the carbohydrate units of IgA1 immunoglobulin. II. Structure of the O-glycosidically linked oligosaccharide units.


Lipogenesis by acini from mammary gland of lactating rats.


The interaction of polypeptide components of human high density serum lipoprotein with detergents.


The decline of molecular activity of cytochrome oxidase during purification.


Studies on ribonucleic acid ligase. Characterization of an adenosine triphosphate-inorganic pyrophosphate exchange reaction and demonstration of an enzyme-adenylate complex with T4 bacteriophage-induced enzyme.


Ornithine cyclase (deaminating). II. Properties of the homogeneous enzyme.


Activation of hexose transport by concanavalin A in isolated brown fat cells. Effects of cell surface modification with neuraminidase and trypsin on lectin and insulin action.


Studies on calciferol metabolism. IX. Renal 25-hydroxy-vitamin D3-1 hydroxylase. Involvement of cytochrome P-450 and other properties.


An iron-containing phosphatase induced by progesterone in the uterine fluids of pigs.


Regulation of 25-hydroxyvitamin D3-1-hydroxylase in vivo.


Formaldehyde dehydrogenase from human liver. Purification, properties, and evidence for the formation of glutathione thiol esters by the enzyme.


Hydroxylysine-deficient skin collagen in a patient with a form of the Ehlers-Danlos syndrome. Two sibs with the Ehlers-Danlos syndrome, one of whom was shown to have hydroxylysine-deficient collagen, are described. In addition to the usual features of the Ehlers-Danlos syndrome (loose-jointedness and excessively stretchable, fragile, and bruisable skin), these patients had severe scoliosis and fragility of ocular tissues leading to rupture of the globe or retinal detachment. This combination of symptoms was tentatively classified as Ehlers-Danlos syndrome, Type VI. The condition is inherited as an autosomal recessive. The activity of lysyl hydroxylase was present at a reduced level in fibroblasts cultured from the patient's skin.


Food vacuole membrane growth with microtubule-associated membrane transport in Paramecium. Evidence from a morphological study of the oral apparatus of Paramecium caudatum using electron microscope techniques have shown the existence of an elaborate structural system which is apparently designed to recycle digestive-vacuole membrane. Disk-shaped vesicles are filtered out of the cytoplasm by a group of microtubular ribbons. The vesicles, after being transported to the cytostome-cytopharynx region in association with these ribbons, accumulate next to the cytopharynx before they become fused with the cytopharyngeal membrane. This fusion allows the nascent food vacuole to grow and increase its membrane surface area. The morphology of this cytostome-cytopharynx region is described in detail and illustrated with a three-dimensional drawing of a portion of this region and a clay sculpture of the oral apparatus of Paramecium. Evidence from the literature for the transformation of food vacuole membrane into disk-shaped vesicles both from condensing food vacuoles in the endoplasm and from egested food vacuoles at the cytoproct is presented. This transformation would complete a system of digestive vacuole membrane recycling.


Studies on dispersed pancreatic exocrine cells. I. Dissociation technique and morphologic characteristics of separated cells. A procedure for dissociation of the guinea pig pancreas into individual cells is described which employs enzymatic digestion with pure collagenase, chymotrypsin, and hyaluronidase, utilizes an interposed chelation of divalent cations by EDTA, and is terminated by gentle shearing. Yields of cells are 50-60%, based on DNA recovered. The population comprises approximately 95% exocrine cells, the remainder consisting of endocrine, duct, and vascular endothelial cells. The exocrine cells, though spherical, retain the structural attributes of their in situ counterparts, including differentiation of the plasmalemma into zones corresponding to the former apical and basal plasmalemma, polarized distribution of organelles indicated by fields of zymogen granules in the cytoplasm underlying the former apex, central location of the Golgi complex, and placement of the rough endoplasmic reticulum and nucleus in the former basal pole of the cell. Electron microscope study of the effects of individual treatments used during dissociation indicates that digestion of basement membrane and collagen is solely due to collagenase activity and that separation of desmosomes (and possibly of zonulae adherentes) results only from exposure to low [Ca(++)] and EDTA and is not effected by the enzymes used. Gap junctions are resistant to enzymes and EDTA; tight junctions resist enzyme treatment but undergo rearrangement upon exposure to EDTA. Both junctions require mechanical shear for complete cell separation. Neither chymotrypsin nor hyaluronidase produces visible alterations in stromal or junctional elements. Dissociation requires the concerted action of enzymes, chelation of divalent cations, and mechanical shear, since the individual treatments are alone ineffective.


Effects of cytochalasin B on the response of toad urinary bladder to vasopressin. A combined physiological and morphological study of the effects of cytochalasin B (CB) on the toad urinary bladder has been carried out. CB inhibits the hydro-osmotic response to vasopressin without altering basal water permeability or diffusion, or the increase in (3)H(2)O diffusion observed after hormone addition. Although CB increases [(22)Na]-, [(36)Cl]-, and [(14)C]urea fluxes, and decreases transepithelial potential, no alteration in basal short-circuit current, the vasopressin-induced increase in this parameter, or [(14)C]inulin permeability occurs. In the absence of hormone, CB does not markedly alter the structure of the toad bladder. However, in the presence of vasopressin, CB induces the formation of large intracellular vacuoles. These results suggest a possible coupling of solute and water movement across the tissue.


Metabolic cooperation in cell culture: studies of the mechanisms of cell interaction.


Analysis of the pinocytic process in rat kidney. I. Isolation of pinocytic vesicles from rat kidney cortex. Pinocytosis was induced in rat kidney by exposure to horseradish peroxidase (HRP). Pinocytic vesicle preparations were enriched after homogenization of kidney cortex by differential centrifugation and free-flow electrophoresis with HRP as an exogenous marker. Vesicles were identified by enzymatic analysis and by electron microscopy, including specific staining procedures. Typical brush-border enzymes such as alkaline phosphatase, aminopeptidase, 5'-nucleotidase, lysosomal acid phosphatase, and mitochondrial succinic dehydrogenase were reduced in the vesicular fraction, compared to the kidney cortex homogenate. Glucose-6-phosphatase and Na(+)-K(+)-ATPase were only slightly increased in the fraction. These results indicate that preparations of pinocytic vesicles from rat kidney cortex can be enriched. They have biochemical characteristics that differ from those of the cell organelles and membranes previously purified from renal tissue.


Release of cytoplasmic enzymes into culture fluid.


In vitro response of goldfish (Carassius auratus L.) dermal melanophores to cyclic 3',5'-nucleotides, nucleoside 5'-phosphates and methylxanthines.


Extracorporeal circulation with and without magnesium. An experimental study.


Effect of cAMP on nucleoside metabolism. II. Cell cycle dependence of thymidine transport.


Cell cycle dependent modulations of the surface membrane of normal and SV40 virus transformed 3T3 cells.


A temperature-sensitive cell cycle mutant of the BHK cell line.


Cellular binding of 3H-cytochalasin B.

Cellular binding of 3H-cytochalasin B.


Concanavalin A and the initiation of thymic lymphoblast DNA synthesis and proliferation by a calcium-dependent increase in cyclic GMP level.


Agglutination by concanavalin A of normal chick embryo fibroblasts treated by 5-bromodeoxyuridine (BrdU).


Editorial: Hypothalmic neurohumors and pituitary tumors.


Bile acid kinetics in relation to sex, serum lipids, body weights, and gallbladder disease in patients with various types of hyperlipoproteinemia;. Bile acid kinetics were determined in 15 normolipidemic and 61 hyperlipidemic subjects with the aid of [(14)C]cholic acid and [(3)H]chenodeoxycholic acid. The diet was standardized and of natural type. The total bile acid formation was within normal limits in patients with hyperlipoproteinemia types IIa and IIb. On the average the production of cholic acid (C) represented less than 50% of the total bile acid synthesis in both groups. The corresponding value recorded for the controls was 64+/-2% (mean+/-SEM). The synthesis of C in hyperlipoproteinemia type IIa was significantly below normal. Of the 27 patients with the type IV pattern, 18 had a synthesis of C and C + chenodeoxycholic acid (CD) that exceeded the upper range recorded for the controls. In these subjects the C formation represented 73+/-3% of the total bile acid synthesis. Similar findings were also encountered in the five patients with the type V lipoprotein pattern studied. The bile acid pool size of the 11 patients with hyperlipoproteinemia type IV, who had been cholecystectomized or suffered from cholelithiasis, was 900 mg smaller on the average than that of the other subjects with the same type of hyperlipoproteinemia. However, the pool size in the former subjects still tended to be higher than that of the control subjects without evidence of gallbladder "disease". In all groups of subjects the formation of bile acids tended to be higher in the male than in the female subjects. Bile acid synthesis showed no linear correlation to actual body weight, relative body weight, or body surface area. A moderate weight reduction in five patients (one with type IIb and four with type IV pattern) was followed by a 50% reduction of the C and CD synthesis.


The secondary structure of human Hageman factor (factor XII) and its alteration by activating agents. Hageman factor (factor XII) is activated by exposure to surfaces such as glass or by solutions of certain compounds, notably ellagic acid. Changes in the structure of Hageman factor accompanying activation have been examined in this study by circular dichroism spectroscopy. The spectrum of unactivated Hageman factor in aqueous solutions suggests that its conformation is mainly aperiodic. Various perturbants altered the conformation of Hageman factor in differing ways, demonstrating the sensitivity of Hageman factor to its environment. After activation of Hageman factor with solutions of ellagic acid, a negative trough appeared in the region of the circular dichroism spectrum commonly assigned to tyrosine residues, along with other minor changes in the peptide spectral region. Some of these changes are similar to changes that occurred upon partial neutralization of the basic residues at alkali pH. Activation of Hageman factor by adsorption to quartz surfaces (in an aqueous environment) also produced changes similar to those in the ellagic acid-activated Hageman factor, including the negative ellipticity in the tyrosine region. These observations suggest that the activation process may be related to a change in status of some of the basic amino acid residues, coupled with a specific change in the environment of some tyrosine residues. The importance of these changes during the activation process remains to be determined. The sensitivity of Hageman factor to its environment is consistent with the view that the initiation of clotting by exposure of plasma to appropriate agents is brought about by alterations in the conformation of Hageman factor that occur in the apparent absence of Fletcher factor or other recognized clotting factors.


Letter: Dynamics and thermodynamics of axial ligation in metalloporphyrins. 3. Effects of molecular interaction of ferric porphyrins with an aromatic acceptor.


Superficial and deep juxtaglomerular apparatus renin activity of the rat kidney. Effect of surgical preparation and NaCl intake. The intrarenal gradient of renin activity was determined in rats by using superficial (S) and deep (D) cortical juxtaglomerular apparatuses (JGA's), identified and microdissected after silicone-rubber compound injection. Angiotensin generated from single JGA's using partially purified sheep renin substrate was quantified by rat bioassay. When, in rats on a normal NaCl diet, silicone-rubber was injected into a carotid artery, alone or with abdominal aorta catheterization, S:D renin activity ratios were 1.18+/-0.08 (SEM) and 1.21+/-0.12, respectively. The S:D renin activity ratios obtained when silicone-rubber was injected into the abdominal aorta (2.52+/-0.09) or a chronic carotid artery catheter (3.44+/-0.40) were significantly higher (P < 0.001). The lower S:D renin activity ratios after carotid artery manipulation were due to significantly higher D-JGA renin activities. This increased D-JGA renin activity and the lack of a renin gradient appear to be related to acute carotid artery manipulation. Alterations in JGA renin activity were examined relative to NaCl intake. 2 wk after high-NaCl diet the absolute net renin activity decreased (P < 0.001) more in S (5.84+/-0.11 ng AI.JGA(-1).h(-1)) than D (1.73+/-0.06 ng AI.JGA(-1).h(-1)) JGA's, and the intrarenal renin gradient was lost (S:D-JGA renin activity, 1.00+/-0.07), as compared to the regular NaCl diet. 2 wk of a low-NaCl diet resulted in a greater (P < 0.01) increase in S (14.28+/-1.47 ng AI.JGA(-1).h(-1)) than D (9.62+/-1.19 ng AI.JGA(-1).h(-1)) JGA renin activity and a renin gradient (S:D-JGA renin activity) of 1.75+/-0.12. These results demonstrate that NaCl intake clearly influences total JGA renin content and may also affect the relative intrarenal distribution of renin activity.


Letter: Lanthanide porphyrin complexes. A potential new class of nuclear magnetic resonance dipolar probe.


Glucagon secretion from the perfused rat pancreas. Studies with glucose and catecholamines. The isolated in situ perfused rat pancreas was used to study glucose and catecholamine control of glucagon secretion, and to investigate the possible role of endogenous cyclic AMP as a mediator of this secretory process. When perfusate glucose was acutely dropped from 100 to 25 mg/100 ml, glucagon was released in a biphasic pattern with an early spike and a later plateau-like response. 300 mg/100 ml glucose suppressed glucagon secretion to near the detection limit of the radioimmunoassay (15 pg/ml). When perfusate glucose was dropped from 300 to 25 mg/100 ml, a delayed, relatively small peak occurred suggesting persisting alpha cell suppression by prior high glucose exposure. 2-Deoxy d-glucose stimulated glucagon secretion and inhibited insulin secretion. Glucagon was secreted in a biphasic pattern in response to both 2.7 x 10(-7) M epinephrine and norepinephrine. The glucagon response to epinephrine was markedly suppressed by glucose at 300 mg/100 ml, and the biphasic response pattern was obliterated. Glucose evoked a two-phase insulin secretory pattern, and the second phase was markedly and rapidly inhibited by epinephrine. Pancreases were perfused with glucose at 300 mg/100 ml which was then lowered to 80 mg/100 ml. 5 min later, epinephrine was infused and definite blunting of the first-phase spike occurred. 10 mM theophylline produced modest rapid uniphasic stimulation of glucagon release, and, in addition, caused enhancement of epinephrine-stimulated glucagon release. An inhibitory influence upon epinephrine-stimulated glucagon was observed as well. Insulin secretion was stimulated by 10 mM theophylline, and this stimulation was inhibited by epinephrine.


Growth and development of children maintained on total parenteral nutrition in the neonatal period.


AMWA--six decades of progress (in the serivice of women in medicine).


Future aspects of the American Medical Women's Association.


Malnutrition and infection. Malaria, cholera and influenza in Africa.


Edith L. Potter, M.D.--pathologist.

Edith L. Potter, M.D.--pathologist.


Letter: To: Women concerned about health care.


A heat-digestible cell-immunoadsorbent made by coupling hapten to gelatin.


Drug induced depression.

Drug induced depression. Some of the drugs that tend to cause mental depression are antihypertensive drugs, ACTH and cortisone, and oral contraceptives (OCs).  OCs containing progestogen-estrogen cause depression on occasion.  Various mechanisms have been invoked to explain this contraceptive hormone induced depression.  Although interference with tryptophan metabolism has been advanced, depression seems to be consequent to the accelerated monoamine resulting in its decreased availability at the synaptic level.  Baumblatt and Winston recommended pyridoxine 50 mg daily to overcome psychiatric effects.


Amoebiasis with pericardial involvement.


The post-mortem diagnosis of Japanese encephalitis.


Seminars from Riley Children's Hospital. Recent developments in treatment of childhood cancer. II.


Testes structure in normal and parthenogenetic turkeys.


Pseudomonas tracheobronchitis. The use of systemic gentamicin and polymyxin-B aerosol.


Fasting hypoglycemia. Diazoxide as a test for endogenous insulin release.


The primary hyperlipoproteinemias.

The primary hyperlipoproteinemias.


Intracellular actions of 5-hydroxytryptamine on the bivalve myocardium. II. Cyclic nucleotide-dependent protein kinases and microsomal calcium uptake.


Characteristics of enterotoxigenic Clostridium perfringens type A isolated from cattle and chickens.


Improved culture techniques and sporulation medium for enterotoxin production by Clostridium perfringens type A.


Circular resection of the trachea for malignant and benign diseases.


Formation of phospholipase C and theta-haemolysin in pre-reduced media in batch anc continuous culture of Clostridium perfringens type A.


Isoprenoid alcohol kinase--a third butanol-soluble enzyme in Klebsiella aerogenes membranes.


Ultrastructure of a marine Thiobacillus.


Growth inhibition of Acetobacter aceti by L-threonine and l-Homoserine: the primary regulation of the biosynthesis of amino acids of the aspartate family.


The effects of beta-galactosidase activity and cyclic AMP on lactose-accelerated death.


Inhibition by ethidium bromide of the establishment of infection by murine sarcoma virus.


The effect of isatin beta thiosemicarbazone (IBT)-related compounds on IBT-resistant and on IBT-dependent mutants of vaccinia virus.


Aminoacylation of RNA from several viruses: amino acid specificity and differential activity of plant, yeast and bacterial synthetases.


The cytopathic effect of herpes simplex virus on HEp-2 cells as shown by scanning electron microscopy.


Correlation between ultrastructural and electrophysiological signs of degeneration in optic nerve terminals of the cat's lateral geniculate nucleus.


Peripheral serotonin antagonists: failure to antagonize serotonin in brain areas receiving a prominent serotonergic input.


Cyclic AMP and amine effects on phosphorylation of specific protein in abdominal ganglion of Aplysia californica; localization and kinetic analysis.


Activity patterns of catecholamine-containing pontine neurons in the dorso-lateral tegmentum of unrestrained cats.


Antifacilitating and simple following responses in a single motoneuron.


The uptake of 36C1 into astrocytes in tissue culture by a potassium-dependent, saturable process.


The characterization of sphingolipids from neurons and astroglia of immature rat brain.


Phosphatidyl inositol turnover in muscle membranes following denervation.


Hydrolytic enzyme activities in the developing chick central and peripheral nervous systems.


Preparation of synaptosomes and vesicles with sodium diatrizoate.


The cytotoxicity of D-galactosamine in cat spinal cord.


The ultrastructure of retinula cell endings in the compound eye of the crayfish.


Interneurons of the sympathetic ganglia, in organotypic culture. A suggestion as to their function, based on three types of study.


The fate of the axon and its terminal in the Pacinian corpuscle following sciatic nerve section.


Neuroglial cells in the cerebral cortex of rats from young adulthood to old age: an electron microscope study.