Electron-spin resonance studies of the structure and formation of bacterial diazodiphenoquinone pigments. The formation of diazodiphenoquinone pigments during bacterial oxidations of pyridinediols can be accounted for in terms of a free-radical mechanism similar to that operating for oxidations in vitro involving azasemiquinone radicals. Electron-spin-resonance spectra are of great value in pigment structure determinations.


Pyridoxal phosphate-sensitized photoinactivation of glutamate decarboxylase from Clostridium perfringens. 1. l-Glutamate decarboxylase (EC 4.1.1.15) from Clostridium perfringens was inactivated by exposure to visible light at pH6.2. 2. Inactivation does not occur at pH4.6 or in the absence of bound pyridoxal phosphate. 3. On prolonged photo-oxidation six histidine residues per molecule of enzyme were destroyed. 4. The loss of six cysteine residues per molecule occurred both in irradiated samples and in controls oxygenated in the dark. 5. This dark-oxidation of cysteine residues is apparently required before the photo-oxidation process. 6. The absorbance, fluorescence and circular-dichroism properties of the enzyme as well as its elution volume during Sephadex gel-filtration were unaffected by prolonged irradiation. 7. However, an apparently homogeneous product of photo-oxidation could be separated from the control enzyme by ion-exchange chromatography. 8. The K(m) for l-glutamate was unchanged in an irradiated sample retaining 22% of control activity. 9. These data and the catalytic role of imidazole residues at the active sites of amino acid decarboxylases are discussed.


The existence of an electrophilic component in the reaction catalysed by triose phosphate isomerase. In the presence of triose phosphate isomerase, the substrate dihydroxyacetone phosphate is reduced stereoselectively by NaBH(4). The reduction of enzyme-bound substrate is almost completely or completely stereoselective and occurs about one order of magnitude faster than that in free solution. This acceleration implies a polarization of the carbonyl group when dihydroxyacetone phosphate is bound.


Characterization of 3-deoxy-D-manno-octulosonic acid as a component of the capsular polysaccharide antigen from Neisseria meningitidis serogroup 29-e.


Induction of N-acetylglucosamine kinase in yeast. The presence of N-acetylglucosamine is essential for the induced synthesis of N-acetylglucosamine kinase in Candida albicans. The enzyme synthesis stops and its concentration in the cells declines rapidly as soon as N-acetylglucosamine is removed from the medium. Experiments with RNA- and protein-synthesis inhibitors indicate that the appearance of new enzyme activity is dependent on concomitant new protein synthesis and the inducer operates at a transcriptional level.


Infidelity of DNA synthesis: a general property of RNA tumor viruses.


Preparation and properties of bovine heart cytochrome c oxidase.


Reversible redox titrations of cytochrome c and cytochrome c oxidase using detergent solubilized electrochemically generated mediator-titrants.


Amino acid sequence of a (32P) phosphopeptide from pig liver pyruvate kinase phosphorylated by cyclic 3',5'-AMP-stimulated protein kinase and gamma-(32P)ATP.


Activation of phosphorylase phosphatase by a novel procedure: evidence for a regulatory mechanism involving the release of a catalytic subunit from enxyme-inhibitor complex(es) of higher molecular weight.


Lack of correlation between fibrinolysis and the transformed state of cultured mammalian cells.


Animal collagenases: specificity of action, and structures of the substrate cleavage site.


A specific binding protein for 17beta-estradiol in retired breeder rat ventral prostate.


Novel heme: O2 bonding of possible relevance to oxygen utilizing heme and other proteins.


Reactivity of cytochrome oxidase with ascorbate.


Studies of inhibition of the luteinizing hormone-releasing hormone by an irreversible inhibitor at the receptor site.


Regulation of pyridoxal 5'-phosphate metabolism in liver.


Regulation of the mitochondrial oxidation-reduction state in intact hepatocytes by calcium ions.


Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells.


Poly(ADP-ribose) synthesis and cell division.


Incorporation of (14C)-2 deoxy-D-glucose into the lipids of normal cells as compared to virus-transformed cells.


Cyclic AMP-induced tyrosinase synthesis in Neurospora crassa.


Poly(U) in poliovirus minus RNA is 5'-terminal.


Correlation between mitogenicity and stimulation of calcium uptake in human lymphocytes.


Location and nucleotide sequence of the site on SV40 DNA methylated by the ECO B modification methylase.


Soluble and bound protein kinases of rabbit gastric secretory cells.


De-esterification of cholesteryl esters in human plasma alpha-lipoprotein (HDL) by preparations of staphylococcal alpha toxin.


Evidence for glucocorticoid transport through the target cell membrane.


Effects of maleylation on the lipid-binding and immunochemical properties of human plasma high density apolipoprotein-A-II.


Comparison of RNA polymerase II from uninfected and simian virus 40-infected permissive CV-1 cells.


Protein kinase activity during development of the insect Ceratitis capitata.


Reconstitution of horse heart cytochrome c: reformation of the peptide bond linking residues 65 and 66.


CDP-choline reversal of the CMP and CTP inhibition of phosphatidic acid synthesis by rat brain preparations.


Electroencephalographic study for the evaluation of the effects of 7-chloro-5-phenyl-3H-1,4-benzodiazepine-2-(1H)-one (N-demethyldiazepam) on sleep disturbances in a group of mental patients.


Erythrocytosis in hepatocellular carcinoma: a compensatory phenomenon.


The presence of O-2'-methylpseudouridine in the 18S + 26S ribosomal ribonucleates of wheat embryo.


Phosphorylation of endogenous substrates by erythrocyte membrane protein kinases. II. Cyclic adenosine monophosphate-stimulated reactions.


Effect of ribose O(2')-methylation on the conformation of nucleosides and nucleotides.


Base analysis of RNA by 3H postlabeling--a study of ribothymidine content and degree of base methylation of 4 S RNA.


The primary structure of yeast glutamic acid tRNA specific to the GAA codon.


Aminoacylation of Escherichia coli valine transfer RNA after oxidation and reduction.


Association of an estradiol receptor with the DNA-dependent RNA polymerase I from immature quail oviduct.


Psychomotor activity, sleep, and biogenic amine metabolites in depression.


Glycoproteins and brain circuitry: the "Sign-Post" theory in normal memory function and in the regressive states of brain tumors and the psychoses.


Seprmiogenesis and cytochemistry of the functional spermatozzon of the rotifer Asplanchna brightwelli.


Effects of ACTH on ovarian histochemistry and maintenance of pregnancy in deermice.


Temperature acclimation and the nervous system.


Clinical significance of estrogen receptors in human breast cancer.


The resting cell: a chemotherapeutic problem. Part II.


Synthesis of plasma beta 2 glycoprotein I by human hepatoma cells in tissue culture.


XC-cell fusion induced by murine plasmocytoma cells. I. Alternate "switching off" and "switching on" by "in vivo"-"in vitro" transfers.


Biochemical approaches to the nosology of nervous system defects, II. The nature of the metabolic defects in all of the major lipid storage diseases has been established within the past four years. This infromation has come primarily from extensive enzymologic studies in human tissues using labeled complex lipids as substrates. A brief description is presented of the background and experiments which led to the identification of the biochemical lesion in Gaucher's disease, Niemann-Pick disease, Fabry's disease, and Tay-Sachs disease.


Worker environment research. II. Sampling and processing techniques for determining dislodgable pesticide residues on leaf surfaces.


Viral infections and malformations of the nervous system. Experimental viral infections are described which cause malformations of the developing nervous system. These include influenza virus infection of chick embryos causing defects in neural tube closure and flexion, parvovirus infections of rodents and cats resulting in a granuloprival cerebellar malformation, and myxovirus infections of rodents inducing stenosis of the aqueduct of Sylvius. In each experimental model the pathogenesis of the malformation is different, but in each the resultant noninflammatory malformation bears resemblances to malformations in man considered to have a genetic basis.


A feasibility study of miniature silica gel columns for the separation of some polychlorinated biphenyls, DDT, and analogs.


A feasibility study of miniature Florisil columns for the separation of some chlorinated pesticides.


Some experiments of nature with sex.

Some experiments of nature with sex.


Protection by phentolamine against the effects of phenoxybenzamine on transmitter release elicited by nerve stimulation in the perfused cat heart. 1 The effects of cocaine, phentolamine and phenoxybenzamine on neuronal uptake of [(3)H]-noradrenaline and on (3)H-transmitter and noradrenaline overflow elicited by nerve stimulation were determined in the perfused heart of the cat.2 During perfusion with cocaine 3.4 x 10(-7)M, there was a 2-fold increase in transmitter overflow while neuronal uptake of [(3)H]-noradrenaline was inhibited by 31.3 +/- 2.1%.3 After exposure to phenoxybenzamine 8.7 x 10(-7)M for 20 min and washing with drug-free solution for 165 min there was an 8-fold increase in transmitter overflow during nerve stimulation. Under these conditions neuronal uptake of [(3)H]-noradrenaline was inhibited by only 17.5 +/- 5.4%.4 There was no significant change in transmitter overflow or in neuronal uptake of [(3)H]-noradrenaline, 155 min after a 30 min exposure to phentolamine (3.2 x 10(-5)M).5 Perfusion with phentolamine (3.2 x 10(-5)M) before and during exposure to phenoxybenzamine (8.7 x 10(-7)M), prevented the increase in transmitter overflow observed after perfusion with phenoxybenzamine alone.6 Protection by phentolamine against the effects of phenoxybenzamine supports the view that the effects on transmitter release obtained after perfusion with phenoxybenzamine are due to the blockade of presynaptic alpha-adrenoceptors which regulate transmitter release through a negative feed-back mechanism.


Drug-induced changes in capillary filtration coefficient and blood flow in the innervated small intestine of the anaesthetized cat. 1 A modification of the Folkow Technique for simultaneous measurement of blood flow and capillary filtration coefficient (CFC) in the cat jejunum is described. The modification retained the sympathetic innervation of the preparation, and in the present experiments, drugs were administered intravenously.2 There is evidence that CFC is a cardiovascular quantity independent of blood flow or regional vascular resistance in these preparations. Low doses of drugs may affect CFC without altering the blood pressure, blood flow, or heart rate.3 Under control conditions the CFC, a measure of functional exchange vessel area, was lower than previously reported for similar, but denervated preparations.4 alpha-Adrenoceptor stimulation with phenylephrine (1.0 mug kg(-1) min(-1), i.v.) caused a fall of 75-85% from control values of CFC with concomitant rises in blood pressure of 0-15% and falls in blood flow of 10-40%. The heart rate rose by 0-15%. Phentolamine (0.5-2.0 mg/kg, i.v.) caused a rise in CFC and a slight fall in vascular resistance, and blocked the effects of phenylephrine on this tissue.5 beta-Adrenoceptor stimulation with isoprenaline (0.2 mug kg(-1) min(-1), i.v.) caused a rise in CFC of 75-110%, a fall in blood pressure of 0-10%, a rise in blood flow of 10-60% and a rise in heart rate of up to 35%. Propranolol caused a transient rise in CFC when injected i.v. in a dose of 0.1 mg/kg, which was adequate to block the effects of isoprenaline.6 Angiotensin (25-100 ng kg(-1) min(-1), i.v.) caused falls in CFC of up to 100% and rises in vascular resistance. Aminophylline (0.2-0.4 mg kg(-1) min(-1), i.v.) caused rises in CFC of up to 200% with falls in vascular resistance.7 Histamine (0.01 to 1.0 mug kg(-1) min(-1), i.v.) had little effect on vascular resistance, but 10 and 40 mug kg(-1) min(-1) caused falls in vascular resistance. Doses up to and including 10 mug kg(-1) min(-1) caused falls in CFC, but the higher doses, or smaller doses after histaminase inhibition caused rises in CFC. alpha-Adrenoceptor blockade reversed the fall in CFC caused by small doses of histamine, to a rise. Mepyramine completely blocked the effects of histamine on these preparations.8 5-Hydroxytryptamine (33-100 mug kg(-1) min(-1), i.v.) caused a rise in vascular resistance and a fall in CFC of up to 85%. These effects were blocked by methysergide (250 mug/kg, i.v.).9 Rises in CFC indicate an increase in functional exchange vessel area in the tissue, and falls in CFC a decrease in the area available for vascular exchange. These changes are examined against the possibility of the drugs causing reflex adjustments in sympathetic tone, of systemic deactivation of intravenously administered drugs, and of drug effects on vascular permeability.


Letter: Urinary cyclic AMP and depression.


Monosynaptic excitation and inhibition of spinal motoneurons by supraspinal structures in the pigeon.


Effects of prostaglandin E1 and 15-methyl- prostaglandin E1 on the cardiovascular responses to bilateral carotid arterial occlusion.


Cell interaction at the maternal-embryonic interface during implantation in the mouse.


Changes in adenylate cyclase, cyclic AMP, and protein kinase levels in chick myoblasts, and their relationship to differentiation.


Evidence for genetic recombination between endogenous and exogenous mouse RNA type C viruses.


Increased protease levels in transformed cells: a casein overlay assay for the detection of plasminogen activator production.


The effect of specific glycosidase treatment on the responsiveness of lymphocytes. I. Response of human lymphocytes to concanavalin-A.


Generation of thymus-derived helper cells by macrophage-associated antigen.


The mechanism of action of soluble lymphocytic mediators. II. Modification of macrophage migration and migration inhibitory factor action by drugs, enzymes and cationic environment.


Immune mechanisms by which the spread of viral infections is stopped.


Cylindroma of the maxilla.

Cylindroma of the maxilla.


In vitro correction of antigen-induced immune suppression: effects of histamine, dibutyryl cyclic AMP and cholera enterotoxin.


Virus content of smallpox scabs.

Virus content of smallpox scabs. Log titres of smallpoxvirus (pock-forming units per 0.1 g of scab) were estimated in scabs from 5 patients from the 13th to the 24th days of illness. They were found to vary from 3.72 to 6.54 with no diminution as convalescence progressed, and they were not related to the primary vaccination status of the patient or the clinical severity of the disease.


The variability in immunofluorescent viral capsid antigen antibody tests in population surveys of Epstein-Barr virus infections. A comparative study of the extent of Epstein-Barr virus (EBV) infections in populations that differ with respect to the incidence of tumours associated with this virus is now in progress in different countries. In these surveys of antibody titres from the various study populations, it is of critical importance that strict comparability be maintained. Despite standardization of techniques and reagents in the cooperating laboratories, considerable variation in the results has remained. The components of the total variability in the results of the immunofluorescence test for estimating the antibody titres against viral capsid antigens (VCA) of the EBV have been investigated. With repeated tests on the same sera, four sources of variation were measured: the reading of the slides, the performance of the tests, the use of various batches of the same cell line as antigen, and the use of different cell lines. The greatest variations were due to the use of different cell lines and to differences in performing the test; the reading of the slides caused only minor variations. Both the systematic and unsystematic variations were measured. The systematic variation was great in tests between laboratories and when different cell lines were used as antigens. Most of the systematic variation resulting from the use of different cell batches from the same cell line could be accounted for by the differing proportions of brilliant fluorescent cells. Adjustments are possible to correct the systematic variation whenever this has been measured, but not the unsystematic "residual" variability, which presents the real obstacle to the comparison of results obtained in different laboratories or by different observers. To attain full comparability of VCA antibody tests the sera from the different surveys should all be tested in the same laboratory.


Plasma lipoproteins and atherosclerosis in man: an immunohistochemical study.


A sleep information storage and retrieval system (SISAR).


Pathogenicity of protein A mutants of Staphylococcus aureus.


The taxonomy of Staphylococcus epidermidis.


Biological activities of Succinic Oxidase Factor, a toxic complex produced by Staphylococcus aureus.


Electrophoresis of proteins.

Electrophoresis of proteins.


Effect of heat activation on biomorphics, hemolysis and lecithinase activity of Clostridium perfringens type A.


Isolation of avian viruses from the eggs of Raillietina tetragona and Ascaridia galli.


Perchlorate and renal excretory functions.


The management of alcoholism. Alcohol withdrawal, long-term treatment and after-care.


An intranuclear paracrystalloid body in the gametocytes of Panorpa communis (Mecoptera).


Fatty acid mobilization in rat adipose tissue-- effect of ingestion of vitamin A.


Adenyl cyclase in the pectoral muscle of normal chickens and chickens with hereditary muscular dystrophy.


Activities of enzymes of the citric acid cycle and electron transport chain in the skeletal muscle of normal and dystrophic mice (strain 129).


Influence of pyrophosphate and diphosphonates on rat liver adenylate cyclase.


Enzyme markers in characterization of isolated plasma membranes.


Plasma prolactin, GH, LH, FSH, TSH and testosterone during treatment of prostatic carcinoma with oestrogens.


Phosphorylation of chromosomal and ribosomal proteins and intracellular levels of cyclic 3',5'-adenosine monophosphate and cyclic 3',5'-guanosine monophosphate during amino acid starvation in Landschutz tumour cells.


Increased phospholipase A activity and formation of communicative contacts between Acanthamoeba castellanii cells. Effect of 3',5'-cyclic AMP.


Distribution of surface charge and concanavalin A-binding sites on normal and malignant transformed cells.


Steroid hormones and the chemistry of behavior.


The relation between adenylate cyclase activation and cAMP acculumation in the horse thyroid gland stimulated by thyrotropin.


The role of protein synthesis in adrenocorticotrophin action: effects of cycloheximide and puromycin on the steroidogenic response of isolated adrenocortical cells.


Effects of luteinizing hormone, follicle stimulating hormone, prostaglandin E1 and other hormones on adenosine-3':5'-cyclic monophosphate and testosterone production in rat testis tissues.


Mode of spironolactone anti-androgenic action: inhibition of androstanolone binding to rat prostate androgen receptor.


Lack of chemically induced mutation in repair-deficient mutants of yeast. Two genes, rad6 and rad9, that confer radiation sensitivity in the yeast Saccharomyces cerevisiae also greatly reduce the frequency of chemically-induced reversions of a tester mutant cyc1-131, which is a chain initiation mutant in the structural gene determining iso-1-cytochrome c. Mutations induced by ethyl methanesulfonate (EMS), diethyl sulfate (DES), methyl methanesulfonate (MMS), dimethyl sulfate (DMS), nitroquinoline oxide (NQO), nitrosoguanidine (NTG), nitrogen mustard (HN2), beta-propiolactone, and tritiated uridine, as well as mutations induced by ultraviolet light (UV) and ionizing radiation were greatly diminished in strains homozygous for either the rad6 or rad9 gene. Nitrous acid and nitrosoimidazolidone (NIL), on the other hand, were highly mutagenic in these repair-deficient mutants, and at low doses, these mutagens acted with about the same efficiency as in the normal RAD strain. At high doses of either nitrous acid or NIL, however, reversion frequencies were significantly reduced in the two rad mutants compared to normal strains. Although both rad mutants are immutable to about the same extent, the rad9 strains tend to be less sensitive to the lethal effect of chemical mutagens than rad6 strains. It is concluded that yeast requires a functional repair system for mutation induction by chemical agents.


Meiosis in male Drosophila melanogaster I. Isolation and characterization of meiotic mutants affecting second chromosome disjuction. Two second chromosome, EMS-induced, meiotic mutants which cause an increase in second chromosome nondisjunction are described. The first mutant is recessive and causes an increase in second chromosome nondisjunction in both males and females. It causes no increase in nondisjunction of the sex chromosomes in either sex, nor of the third chromosome in females. No haplo-4-progeny were recovered from either sex. Thus, it appears that this mutant, which is localized to the second chromosome, affects only second chromosome disjunction and acts in both sexes.-The other mutant affects chromosome disjunction in males and has no effect in females. Nondisjunction occurs at the first meiotic division. Sex chromosome disjunction in the presence of this mutant is similar to that of sc(4)sc(8), with an excess of X and nullo-XY sperm relative to Y and XY sperm. In some lines, there is an excess of nullo-2 sperm relative to diplo-2 sperm, which appears to be regulated, in part, by the Y chromosome. A normal Y chromosome causes an increase in nullo-2 sperm, where B(s)Y does not. There is also a high correlation between second and sex chromosome nondisjunction. Nearly half of the second chromosome exceptions are also nondisjunctional for the sex chromosomes. Among the double exceptions, there is an excess of XY nullo-2 and nullo-XY diplo-2 gametes. Meiotic drive, chromosome loss and nonhomologous pairing are considered as possible explanations for the double exceptions.


The locus for human adenine phosphoribosyltransferase on chromosome no. 16. Evidence for assigning the locus determining the structure of adenine phosphoribosyltransferase (APRT) to human chromosome No. 16 is presented. Hybrids of APRT-deficient mouse cells and of human fibroblasts having normal APRT were isolated by fusing the parental cells with Sendai virus, blocking de novo purine nucleotide synthesis with azaserine and selecting for hybrids that could use exogenous adenine. The hybrid clones that were studied had only APRT activity that was indistinguishable from human APRT with regard to electrophoretic migration and reaction with antibodies against the partially purified human enzyme. No. 16 was the only human chromosome consistently present in all of the clones, and in one clone, it was the only human chromosome detected. Selection against hybrid cells with 2,6-diaminopurine (DAP) yielded DAP-resistant survivors that lacked chromosome No. 16. One hybrid that originally had an intact No. 16 yielded adenine-utilizing subclones that lacked No. 16 but had a new submetacentric chromosome. The distribution of centromere-associated heterochromatin and the fluorescence pattern indicated that this chromosome consisted of a mouse telocentric chromosome and the long arm of No. 16. Cells having the submetacentric chromosome had human APRT. Both the enzyme and the chromosome were absent in DAP-resistant derivatives. These results suggest that the structure of APRT is defined by a locus on the long arm of human chromosome No. 16.


Two first degree relatives with dilatation of the colon due to Crohn's disease.


Biochemical parameters of abnormal endometrium.


The changing nature of endometrial cancer.


Factors controlling intracellular levels of estrogens in human endometrium.


Estrogen receptors and the estrone hypothesis in relation to endometrial and breast cancer.


Receptors for progesterone.

Receptors for progesterone.


Estrogen action in the uterus: the requisite for sustained estrogen binding in the nucleus.


Pharmacology of the endometrium.

Pharmacology of the endometrium.


[Control of cell proliferation in haemopoietic tissue].


The yield and characteristics of enzymatically isolated mouse placental cells.


The teaching of zonal junctions.

The teaching of zonal junctions.


Morphological structure of mediaeval skulls excavated on the Baltic coast between the Oder and Vistula rivers.


Pituitary-adrenal activity in the postpartum rat in the absence of suckling stimulation.


Detection of amoebic antigen in liver pus by counter immunoelectrophoresis--a preliminary report.


Studies on the immune response in clinical amoebiasis and in experimental animals.


In vitro comparative evaluation of tinidazole and metronidazole on strains of Entamoeba histolytica.


Studies on ribonuclease S': the role of lysine-7 for activation of S-protein.


Letter: Photosensitization of nucleic acid constituents by proflavine: NO2 radicals due to impurities.


Letter: Radical reactions and thermoluminescence in L-histidine and 5-nitro-uracil.


Synthesis of herpes simplex virus type 1 (HSV-1) DNA in isolated nuclei. II. Covalent linkage of RNA to nascent viral DNA.


Synthesis of herpes simplex virus type 1 (HSV-1) DNA in isolated nuclei. I. Conditions for isolation of nuclei capable of viral DNA synthesis.


Modern biochemical concepts of anxiety. Implications for psychopharmacological treatment.


Identification of hexokinases and localisation of a fructokinase and a tetrazolium oxidase locus in Drosophila melanogaster.


Chromosomal characteristics of neurogenic tumours in adults.


Isozyme patterns in Rous sarcoma virus-induced tumors in the rat.


The relationship of Sendai virus-induced chromosome pulverization to cell cyclus in HeLa cells.


The chromosomes of fifty primary Rous rat sarcomas.


Comparative chromosome analysis of primary and metastatic Rous sarcomas in rats.


Letter: Utilization of 13C-13C coupling in structural and biosynthetic studies. IV. Penicillic acid.


Chromosomes of six primary sarcomas induced in the Chinese hamster by 7,12-dimethylbenz(a)anthracene.


5-Bromouridylyl-(3-5)-adenosine isolated from 5-bromouracil-induced filaments of Escherichia coli. A dinucleoside monophosphate was isolated from 5-bromouracil-induced filaments of a thymine auxotroph of Escherichia coli K-12. The dinucleoside monophosphate was fractioned from a [(14)C]5-bromouracil-labeled perchloric acid extract using Dowex-1-formate ion-exchange chromatography. Sephadex chromatography revealed its molecular weight to be 710. Snake venom phosphodiesterase digest of the dinucleoside monophosphate yielded [(14)C]5-bromouridine and adenosine 5'-monophosphate. The presence of [(14)C]5-bromouracil in bacterial ribonucleic acid indicates that ribonucleic acid, which had incorporated 5-bromouracil, was the probable source of this dinucleoside monophosphate, 5-bromouridylyl-(3' --> 5')-adenosine.


Catabolite repression and derepression of arylsulfatase synthesis in Klebsiella aerogenes. When a mutant (Mao(-)) of Klebsiella aerogenes lacking an enzyme for tyramine degradation (monoamine oxidase) was grown with d-xylose as a carbon source, arylsulfatase was repressed by inorganic sulfate and repression was relieved by tyramine. When the cells were grown on glucose, tyramine failed to derepress the arylsulfatase synthesis. When grown with methionine as the sole sulfur source, the enzyme was synthesized irrespective of the carbon source used. Addition of cyclic adenosine monophosphate overcame the catabolite repression of synthesis of the derepressed enzyme caused by tyramine. Uptake of tyramine was not affected by the carbon source. We isolated a mutant strain in which derepression of arylsulfatase synthesis by tyramine occurred even in the presence of glucose and inorganic sulfate. This strain also produced beta-galactosidase in the presence of an inducer and glucose. These results, and those on other mutant strains in which tyramine cannot derepress enzyme synthesis, strongly suggest that a protein factor regulated by catabolite repression is involved in the derepression of arylsulfatase synthesis by tyramine.


Round-cell mutant of Salmonella typhimurium. A mutation at the divD locus in Salmonella typhimurium confers a round-cell morphology and enhances cell division under certain growth conditions.


Coenzyme M, essential for growth of a rumen strain of Methanobacterium ruminantium. A growth factor present in rumen fluid and essential for growth of a rumen strain of Methanobacterium ruminantium was shown to be coenzyme M, 2-mercaptoethanesulfonic acid.


Amino acid sequence of an alkaline proteinase inhibitor (Streptomyces subtilisin inhibitor) from Streptomyces albogriseolus S-3253.


Systems of virus survival.

Systems of virus survival.


Effect of cyclic guanosine 3,5-monophosphate on the synthesis of enzymes sensitive to  caatabolite repression in intact cells of Escherichia coli. Cyclic guanosine 3',5'-monophosphate inhibits the synthesis of beta-galactosidase and tryptophanase in cultures of Escherichia coli growing in minimal media with glucose or glycerol as the carbon source. Cyclic guanosine 3',5'-monophosphate acts at the transcriptional level in the lac operon, it exerts its action at the promoter site of the operon, and requires the presence of functional cyclic adenosine 3',5'-monophosphate receptor protein.


Maedi-visna.

Maedi-visna.


Immune responses in persistent virus infections.


Cytomegalovirus: a cause of persistent latent infection.


Feline leukemia.

Feline leukemia.


Persistent infection by adenoviruses.

Persistent infection by adenoviruses.


Varicella-zoster and related viruses.

Varicella-zoster and related viruses.


EB virus in relation to tumours.

EB virus in relation to tumours.


Oncogenic herpesvirus infections of animals.


Dreams and dreaming.

Dreams and dreaming.


The EB virus in relation to infectious mononucleosis.


Carcinoma of the maxillary sinus following thorotrast instillation.


Electrophysical study of peroneal palsy. The diagnostic yield of different electrophysiological criteria was examined to establish whether a peroneal palsy was due to compression of the nerve in the region of the capitulum fibulae. Slowing of sensory conduction along the segment of the nerve across the capitulum fibulae localized the lesion in 64% of 47 consecutive patients with a history indicating or suggesting compression of the nerve in the vicinity of the capitulum fibulae and there were no false positive findings in 18 patients whose peroneal palsy was not due to compression at the capitulum fibulae. In 20% of the patients with slowing along the segment across the capitulum, conduction velocity was normal when measured from the superior retinaculum to the popliteal fossa. Slowing along motor fibres (m. extensor digitorum brevis) localized the site of the lesion in one-third of the patients. Differences in amplitude and in split-up of the sensory responses recorded in the popliteal fossa as compared with those recorded distal to the capitulum fibulae were of limited diagnostic value because of many false positive findings among patients whose peroneal palsy was not due to compression of the nerve at the capitulum fibulae.


Diagnostic difficulties encountered in the myasthenic syndrome sometimes associated with carcinoma. Six patients with the physiological features of the myasthenic syndrome associated with carcinoma are presented. The clinical features encountered in this group, including age, mode of onset, and duration of symptom indicate that it may escape detection owing to its resemblance to other neuromuscular disorders. Conventional concentric needle electromyography may lead to the disorder being confused with a myopathy. The association with carcinoma is by no means constant. Clinical and physiological improvement with guanidine is described in each case.


Proceedings: Possible evidence for lateral inhibition in the somesthetic cortex of the rat.


Proceedings: The effect of thermal stress and frustration on the pituitary-adrenal axis of the young pig.


Myopathy with mitochondrial inclusion bodies: histological and metabolic studies. Apparently new information about a metabolic abnormality has been obtained by measuring biochemical and respiratory responses to controlled exercise in a patient with abnormal mitochondria in muscle fibres. A male patient (49 years old) presented with bilateral ptosis of 15 to 20 years' duration and weakness for one to two years. Biopsies from the deltoid and triceps muscles were subjected to histological, histochemical, and electron microscopical examination. Routine histology showed only minor changes; 2-5% of muscle fibres had pale borders in which there were aggregates of mitochondria and 1-5% of fibres showed atrophy. Histochemical examination showed increased activity of succinic dehydrogenase in mitochondria and lactate dehydrogenase in cytoplasm. Electron microscopy showed crystalline inclusions in many subsarcolemmal mitochondria. Metabolites were studied during and after exercise on an ergometer and revealed remarkable differences from normal. Blood lactate rose to 12·5 μmol/ml and pyruvate to 0·39 μmol/ml compared with up to 4·0 and 0·16 μmol/ml respectively in controls exercising to a comparable percentage of capacity. Concentrations of ketone-bodies and free fatty acids fell during and after exercise, while they rose in the controls. These observations imply a major mitochondrial defect which causes dramatic biochemical changes in fuel supply in blood during exercise. The changes suggest that fat metabolism was accelerated (in this patient) and was related to a block of carbohydrate utilization as fuel.


Persistent modification of synaptic interactions between sensory and motor nerve cells following discrete lesions in the central nervous system of the leech. We have examined changes that develop in the synaptic interactions of sensory and motor nerve cells following surgical lesions to the central nervous system of the leech. In one type of operation an individual ganglion was isolated from the rest of the nervous system by severing all the incoming and outgoing fibres. During the next few weeks, marked changes appeared in synaptic interactions.1. In chronically isolated ganglia inhibitory potentials were recorded in the motoneurone which raises the skin into ridges (the AE cell) following impulses in sensory neurones that respond to pressure (P) or noxious (N) stimuli. In contrast the same AE cell in ganglia taken from normal animals shows excitatory synaptic potentials when the P or N sensory cells are stimulated.2. Another altered synaptic interaction in ganglia isolated by lesions was that between sensory cells responding to touch and a motoneurone that supplies longitudinal muscles (L cell). Instead of the pure, electrical coupling potential seen normally, a large, additional chemically mediated excitatory potential was also apparent.3. Some of the changes in synaptic interactions were not restricted to synapses within the isolated ganglion, but appeared gradually over the following year in successive ganglia along the length of the ventral nerve cord.4. Indirect evidence suggests that the altered synaptic potentials that became conspicuous after operations are also present but smaller and obscured in normal animals.5. It is concluded that some synapses in the leech nervous system are more readily changed than others by cutting the connectives. Furthermore, these changes influence in a predictable manner the way in which the animal behaves in response to mechanical stimuli.


Proceedings: Demonstration of the effect of nucleotide phosphodiesterase and prostaglandin F2alpha on oxytocin-induced milk ejection in the anaesthetized rat with simultaneous recording of intra-uterine pressure.


Localization of beta adrenergic receptors, and effects of noradrenaline and cyclic nucleotides on action potentials, ionic currents and tension in mammalian cardiac muscle. 1. Isoprenaline and noradrenaline were applied iontophoretically to cardiac Purkinje fibres. Intracellular application of the drugs had no effect, while extracellular application of the same amounts of charge caused acceleration of pace-maker activity and a shift of the plateau level of the action potential. These results indicate that beta-adrenergic receptors are located at the outside of the cardiac cell membrane.2. A systematic comparison of the effects of cyclic AMP derivatives and noradrenaline on action potentials and isometric tension of ventricular myocardial preparations showed that the nucleotides and the catecholamine increase the plateau height and the duration of the action potential and also increase tension. However, there are quantitative differences in the action of these drugs.3. Cyclic AMP derivatives and noradrenaline increase the slow inward current, I(Ca), in ventricular myocardial preparations. Voltage clamp analysis of I(Ca) showed that the kinetic parameters of this membrane current are not affected by these drugs. However, the membrane conductance to Ca ions is greatly increased by noradrenaline and to a smaller extent by dibutyryl cyclic AMP.4. Concentration-response relations of the membrane effects of noradrenaline on plateau height of the action potential and on I(Ca) could be fitted by the same theoretical log concentration-response curve. The Hill plot of this concentration-response curve had a slope of 2. The half maximal response occurred at 5 x 10(-7)M.5. The results are compared with other membrane effects of catecholamines and cyclic nucleotides in cardiac muscle. The effects on I(Ca) are related to the positive inotropic effect of the drugs.


Proceedings: The value of high extracellular calcium as a test for monosynaptic connexions in Planorbis corneus.


Proceedings: Synaptic transfer curves at a vertebrate central synapse.


Proceedings: Post-tetanic potentiation of isometric twitch tension is not transferred from one motor unit to another in the same small isolated muscle.


Proceedings: Distribution of acetylcholine and of acetylcholinesterase activity in the nervous tissue of the frog and of the leach.


Proceedings: Cyclic AMP: cell-to-cell movement and inotropic effect in ventricular muscle, studied by a cut-end method.


Casein phosphatase in the developing rat brain.


Note on the activity of plasma angiotensinase in human exercise.


The recovery of hepatic phagocytosis after blockade of Kupffer cells.


Skin reaction and blast transformation of lymphocytes of guinea pigs sensitized with trypsin inhibitors.


Electron microscopical study of cytoplasmic inclusion body in hepatitis B.


Effects of cyclic AMP and dibutyryl cyclic AMP on the heart and coronary circulation.


Studies on a new 1,5-benzothiazepine derivative (CRD-401) VI. Effects on renal blood flow and renal function.


[Actions of papaverine, aspaminol and bile salts and intracellular cyclic AMP level].


Redox state of nicotinamide-adenine dinucleotide in the inner and outer layers of canine left ventricle, and effects of coronary dilators on the redox state.


Modification by external calcium and magnesium ions of the potentiative action of 5'-AMP on the ATP-induced contraction of isolated guinea-pig ileum.


Infectious bursal disease viral particles revealed in avian cell culture by electron microscope.


Unusual pulmonary metastasis of mammary cancer: unilateral pulmonary fibrosis after mammary Paget's disease.


Binding and metabolism of testosterone in the rat brain during sexual maturation. II. Testosterone metabolism.


Binding and metabolism of testosterone in the rat brain during sexual maturation. I. Macromolecular binding of androgens.


Periplasmic steroid-binding proteins and steroid transforming enzymes of Pseudomonas testosteroni.


Binding and metabolism of testosterone and of 5-dihydrotestosterone in bulbocavernous/levator ani (BCLA) of male rats: in vivo and in vitro studies.


The cytoplasmic progesterone receptor of human endometrium during the menstrual cycle. Clinical research was undertaken to determine the amount of high-affinity progesterone binding in human endometrium during the menstrual cycle.  The laboratory procedures involved are described in detail; results are graphed and tabulated.  Human endometrial 20,000 g supernatant was used.  The relative affinities of several synthetic progestins for this endometrial progesterone receptor was also studied.  Hyperplastic endometrium had a significantly higher progesterone binding capacity than proliferative or secretory phase endometrium at the p.01 level.  In the presence of 100 nM cortisol, the amount of progesterone bound was reduced in 1/2 the samples.  As a result of this study, it was concluded that an intact 3-keto-4-ene structure is essential for steroid binding to the receptor.  The nature of the substituents at C-17 can be changed without effect.


Effect of superfusion with human male serum, bovine serum albumin or non radioactive estrogens on the retention of tritiated estradiol-17 beta and estriol by the rat uterus.


Enzymic fluorometric method for the determination of cortisol in plasma.


Modifiers of steroid-hormone metabolism: a review of their chemistry, biochemistry and clinical applications.


On the origin of prokaryotes.

On the origin of prokaryotes.


The second messager. Part I. Cyclic AMP.


A morphological study by freeze-etching of Sendai virus and its interaction with human erythrocytes.


Studies on the positive regulatory gene, GAL4, in regulation of galactose catabolic enzymes in Saccharomyces cerevisiae.


Lipidosis-like ultrastructural alterations in rat lymph nodes after treatment with tricyclic antidepressants or neuroleptics.


Role of calcium and cyclic adenosine 3',5'-monophosphate in the antilipolytic effect of tolbutamide and glibenclamide.


Adrenal cortex adenylate cyclase. Comparison between the action of GTP and 5'-guanylyl-imidodiphosphate on the particulate enzyme from bovine adrenal cortex and rat adrenals.


Induction of skeletal malformations in organ cultures of mammalian embryonic tissues.


Nerve root scarring and arachnoiditis as a complication of lumbar intervertebral disc surgery--surgical treatment.


Synthesis of synaptic glycoproteins. I. The distribution of UDP-galactose: N-acetyl glucosamine galactosyl transferase and thiamine diphosphatase in adult rat brain subcellular fractions.


Mass fragmentographic measurement of norepinephrine dopamine, serotonin and acetylcholine in seven discrete nuclei of the rat tel-diencephalon.


Intrageniculate terminal depolarization of the cat's retinal ganglion cell axons during reserpine-induced PGO-spike.


The effect of tri-iodothyronine in combination with impramine on [3H]-cyclic AMP production in slices of rat cerebral cortex.


Haemocytic changes associated with the immune reaction of nematode-infected larvae of Orthellia caesarion.


ACTH accelerates the formation of cytoplasmic vacuoles in the adrenal cortex of androgen-treated rats. The effect of simultaneous injections of ACTH and methylandrostenediol (MAD), a synthetic androgen, has been studied in the rat. ACTH accelerated the production of cytoplasmic vacuoles by MAD in as much as no such structures were observed in MAD-treated animals at 4 weeks. Adrenal weight was decreased in MAD-treated animals but restored to that of controls by ACTH (MAD plus ACTH group). ACTH by itself did not, however, cause the production of any vacuolar structures nor did it cause any reparation in the number of mitochondrial cristae which were considerably reduced in numbers after MAD plus ACTH.


Impulse discharges from the low pressure side varying with the central blood volume.


Recent changes in bacterial resistance to antibiotics.


Schizophrenia.

Schizophrenia.


Anti-inflammatory drugs in rheumatic diseases.


Virus diseases.

Virus diseases.


Proceedings: Plasma lipoproteins of the suckling lamb compared with adult sheep.


Role of cyclic 3',5'-AMP on contraction and relaxation of perfused rat caudal artery.


Pharmacological actions of 6-hydroxydopamine.


Photosynthetic reaction centers and primary photochemical reactions.


Spectroscopic studies on two-iron ferredoxins.


NMR in cancer. V. Electronic diagnosis of cancer by potassium (39K) nuclear magnetic resonance: spin signatures and T1 beat patterns.


Viruslike structures in systemic lupus erythematosus.


Microfilaments in yeast mitochondria.

Microfilaments in yeast mitochondria.


The stimultaneous use of two prostaglandin E radioimmunoassays employing two antisera of differing specificity. I. Determination of prostaglandin content in sera and culture medium of simian virus 40 transformed cells.


Recent drugs and the rheumatic diseases.


'Nonspecific' blocking of human ovarian carcinoma-associated cellular cytotoxicity in vitro.


The effect of factor IX and factor X on blood platelets.


A micro-culture method for the typing of enteroviruses.


Serum haptoglobin levels in patients with liver diseases.


Clinical sequelae after japanese encephalitis: a one year follow-up study in Thailand.


Effects of collagen on fibrinolysis.

Effects of collagen on fibrinolysis.


Estimation of urinary angiotensin II by radioimmunoassay.


Effects of thalamic midline nuclei stimulation upon lateral geniculate transmission in rats: inhibition of principal cells and excitation of internuncial cells.


Pneumoconiosis in painters dealing with Tonoko: three cases of "Tonoko-lung".


Effect of cytosine arabinoside and two of its analogues on herpes simplex virus type 1 in cell cultures.


Synthesis and properties of some new NAD analogues.


Consequences of spingomyelin degradation in erythrocyte ghost membranes by staphylococcal beta-toxin (sphingomyelinase C).


Effect of cobra venom (Naja haje) on insulin release by rat pancreas in vitro.


Enzymatic activities and other characteristics of Crotalus durissus cumanensis venom.


The azure dyes: their purification and physicochemical properties. I. Purification of azure A.


Correspondence: Metals in tissues.

Correspondence: Metals in tissues.


The biosynthesis of mitochondrial cytochromes.


Expression of the familial hypercholesterolemia gene in heterozygotes: model for a dominant disorder in man.


Epstein-Barr viral nuclear antigen (EBNA) in tumor cell imprints of experimental lymphoma of marmosets.


Renomedullary endocrine system: its antihypertensive action.


Molecular action of vitamin D in the chick intestine.


Some aspects of vitamin D action; calcium absorption and the vitamin D-dependent calcium-binding protein.


Vitamin D binding proteins.

Vitamin D binding proteins.


The hormone-like action of 1,25-(OH)2-cholecalciferol (a metabolite of the fat-soluble vitamin D) in the intestine.


Gastrin.

Gastrin.


The role of estrophilin in estrogen action. The role of estrophilin in estrogen action was reviewed.  The interaction of uterine cells with estradiol involves the association of estradiol with estrophilin followed by temperature-dependent translocation of the resulting complex to the nucleus.  The steroid binding unit of estrophilin undergoes an alteration (receptor transformation) that is recognized by an increase in its sedimentation rate and by its ability to bind to isolated uterine nuclei or chromatin and to alleviate a tissue  specific deficiency in the ribonuclei acid (RNA) synthesizing capacity.  The estrophilin complex influence on RNA synthesis in isolated nuclei shows the same quantitative, qualitative and tissue specificity characteristics as evoked by the administration of estradiol in vivo.  It was concluded that receptor transformation is an important step in estrogen action and that a major role of the hormone is to induce conversion of the native estrophilin to a biochemically functional form.


Production of pollution-tolerant strains of unicellular algae through mutagenic treatment. I. Ethyl methane sulphonate.


Current concepts of viral hepatitis and a peek into the future. New information has prompted revision of the conceptual framework for considering the epidemiology and virology of viral hepatitis. The means are now at hand to identify infections due to either Hepatitis A or B, as well as to implicate other etiologic agents in hepatitis. Immunologic evidence of variation in the antigens associated with Hepatitis B, and possibly in Hepatitis A, may explain some well known epidemiologic phenomena and has important implications in immune serum globulin prophylaxis. The ambiguous relationship of antigenemia and viremia in Hepatitis B is explored in relation to the hepatitis hazard of blood products, to trials of immune serum globulin, and to the potential role of the carrier-health worker in hepatitis transmission. The emerging concept of non-parenteral transmission of Hepatitis B is reviewed and future developments in the production of hepatitis vaccines and in experimental viral hepatitis in non-human primates is briefly discussed.


On a possible cocarcinogenic and immunosuppressive activity of phorbol in AKR-mice.


Chronic exposure to carbon disulfide: effects on occupationally exposed workers with special reference to the nervous system.


In vitro dissolution of three varieties of asbestos in physiological fluids.


Double cancers of the lungs?

Double cancers of the lungs?


Glucose intolerance in a patient with insulinoma. Report of a case.


Primary hyperparathyroidism. Analysis of findings in a series of 129 patients.


Dependence of pancreatic enzyme secretion on extracellular Ca++ and its modification by cyclic nucleotides and thyrocalcitonin.


A simple test for the luteinizing hormone releasing function of hypothalamo-pituitary axis using conjugated estrogen and its clinical evaluation.


Transplantation of the lung: a symposium. Lung grafting and surfactant.


Infantile digital fibromatosis. Histopathological and electron microscopic study with a review of the literature.


The susceptibility of aerobic and anaerobic bacteria, L-phase variants, candida, protozoa and viruses to lysolecithin.


Scirrhous carcinoma occurring in the corpus (body) of the stomach.


N,N'-dimethylnitrosourea-induced tumors in mice and Syrian hamsters.


Isoelectric analysis of haemolysins and enzymes from streptococci of groups A, C and G.


Tumor cell surfaces: general alterations detected by agglutinins.


Virion and tumor cell antigens of C-type rna tumor viruses.


Modulation of cell surface structure and function.


Experimental infection of lambs with reovirus type 1 isolated from sheep.


Biological activities solubilized cell surface components.


Characterization of an ovine strain of parainfluenza-3 virus isolated from sheep showing respiratory disease.


Pneumoenteritis of lambs caused by adenovirus. Preliminary report.


Cross covariance function estimation by an average response computer.


Clinical aspects of hyperlipoproteinemia.


Protein synthesis and insect morphogenesis.


Merkel cells as human cutaneous neuroreceptor cells. Their presence in dermal neural corpuscles and in the external hair root sheath of human adult skin.


Adenyl cyclase activity in the spleen after immunization of neonatally thymectomized mice.


Action of adenosine triphosphate on mast cells in normal and denervated skin.


The inhibitory effects of hydrocortisone on human glioma cells in vitro.


Avian leukosis virus induced tumours in mammals.


Minor RNA and other components of host origin intrinsic to avian leukosis virus particles.


Virological analysis of individual foci induced in hematopoietic cells by avian myeloblastosis in virus.


The RNA of RNA-containing tumor viruses.


Envelope classification of avian RNA tumor viruses.


Evidence for the vertical transmission of 'non-infectious' avian leukosis virus in 'leukosis-free' chickens.


Experiences with complement fixing sera from pigeons in avian leukosis.


The non-producer cell activation test in avian leukosis virus assay.


Identification of leukemogenic viruses: specifications for vertically transmitted, mostly 'switched off' RNA tumor viruses as determinants of the generality of cancer.


Aspects of Harvey murine sarcoma virus complex.


Enzyme studies in experimental leukemia.


Studies on the hamster papilloma and the hamster virus lymphoma.


Induction of murine leukemia with human neoplastic tissue.


High and low leukemogenic variants of Rauscher virus having a comparable infectivity in vitro.


Epidemiology of feline leukemia (lymphosarcoma).


Tests for mouse leukemia viruses and isolation of some of their components.


Serological studies of the feline leukemia virus.


Feline leukemia virus: detection of group-specific viral antigen and infectious virus by a complement-fixation test.


Feline leukemia characterization of the virus.


Growth of feline leukemia virus in human, canine and porcine cells.


Biological studies with viral induced fibrosarcomas in cats, dogs, rabbits and non-human primates.


Induction of tumors in marmoset monkeys with ST-feline fibrosarcoma virus.


Serial cellular transmission of canine lymphoma.


Canine mast cell leukemia.

Canine mast cell leukemia.


Virus association with 90Sr induced leukemia of miniature swine.


Progress on transmission of bovine lymphosarcoma.


Isolation of C-type virus particles from leukemic and lymphocytotic cattle.


Leukemia of guinea pigs.

Leukemia of guinea pigs.


Significance of virus particles observed in spontaneous and induced tumors of the syrian hamster.


Comparisons of virus-like particles from leukotic cattle to feline leukosis virus.


L2C/NB guinea pig leukemia: failure to demonstrate transmissible leukemogenic virus.


The association of herpes-like virus and guinea pig leukemia.


'C'-type virus particles in rat tumors.

'C'-type virus particles in rat tumors.


Neoplastic bone lesions induced in rats and hamsters by Moloney and Harvey murine sarcoma viruses.


Comparison of leukemogenic and sarcomagenic viruses at the ultrastructural level.


Activities of immune lymphoid cells against leukemia virus-carrier murine neoplastic cells.


Current studies on a new 'C'-type virus of presumed reptilian origin.


Evidence for a relation of Epstein-Barr virus to Burkitt's lymphoma and nasopharyngeal carcinoma.


EB virus infection and propagation in human hematopoietic cells.


Occurrence, transmission and oncogenic spectrum of the avian leukosis viruses.


Lymphoproliferative effect of Epstein-Barr virus on normal human lymphocytes in cultures.


Strain MC29, an avian leukosis virus of unique properties.


Genetic studies of human erythrocyte inosine triphosphatase.


Studies of glucuronidation and sulfation in tumor-bearing rats.


Effect of arginine on the secretion of insulin and adenosine 3',5'-monophosphate in the pancreatic venous effluent of trained, nonanesthetized dog.


Effects of delta 9-tetrahydrocannabinol on the levels of cyclic adenosine 3',5'-monophosphate in mouse brain.


Reversibility of hepatic changes caused by ethoxyquin.


Effect of acetone administered in vivo upon hepatic microsomal drug metabolizing activity in the rat.


Changes in the nuclear membrane surface following transformation of chick embryo fibroblasts by rous sarcoma virus: the effect of camptothecin.


The significance of tissue gas and clostridial organisms in the differential diagnosis of gas gangrene.


Effect of exercise and physical fitness on serum lipids and lipoproteins.


Combined hyperlipoproteinemia. Evidence for a new lipoprotein phenotype.


Anterior ischaemic optic neuropathy. I. Terminology and pathogenesis.


Anterior ischaemic optic neuropathy. II. Fundus on ophthalmoscopy and fluorescein angiography.


Anterior ischaemic optic neuropathy.  III. Treatment, prophylaxis, and differential diagnosis.


Hyperlipaemia following phlebotomies. Hyperlipaemia of Fredrickson's type V after phlebotomies for control of erythrocytosis.


Secholex, Clofibrate and taurine in hyperlipidaemia.


Ischaemic papillopathy. Clinical and fluorescein aniographic review of forty cases.


Biochemical properties of oncornavirus polypeptides.


Isolation of nucleolar proteins by ethanol precipitation of nucleic acids.


X-ray structure of thymidine 5'-carboxylic acid, an inhibitor of thymidine and thymidylate kinase: preferred nucleobase-carboxylic acid hydrogen bonding scheme.


Structural studies of avian myeloblastosis virus. Selective release of ribonucleoprotein polypeptides from the core component and partial purification of the DNA polymerase.


Conformation and reactivity of DNA. VI. Circular dichroism studies of salt-induced conformational changes of DNAs of different base composition.


Mucolipidosis. 3. New studies.

Mucolipidosis. 3. New studies.


Apparent autosomal recessive inheritance of the Treacher Collins syndrome.


Familial cardiac lipidosis.

Familial cardiac lipidosis.


A familial syndrome characterized by peculiar facies, small stature, chromosomal aberrations and aminoacriduria.


Relation of c-amp to relaxation of pulmonary artery.


Cyclic amp blood vessels of spontnaeously hypertensive rat.


Chemicals and meat: the embalmed beef scandal of the Spanish-American War.


Effect of converting enzyme blockade on isoprenaline- and angiotensin I-induced drinking. 1 Intravenous infusion of 0.072 mumol kg(-1) h(-1) (1Asp, 5-Ile) angiotensin I, 0.116 mumol kg(-1) h(-1) of (1-Asp beta-amid, 5-Val) angiotensin II or of (1-Asp, 5-Ile) angiotensin II, caused food-deprived and water-satiated rats to drink about 3.0 ml of water per animal. This indicates that angiotensin I has a 1.6 times stronger dipsogenic effect than the angiotensin II preparations when infused intravenously.2 The converting-enzyme-inhibitor SQ 20881 (Pyr-Trp-Pro-Arg-Pro-Gln-Ile-Pro-Pro) (1.0 mg/kg i.v.) had no intrinsic dipsogenic effect.3 SQ 20881 given in combination with angiotensin I or angiotensin II potentiated the dipsogenic effect of angiotensin I, but not that of the two angiotensin II preparations.4 The drinking induced by isoprenaline (100 mug/kg, i.m.) was potentiated by SQ 20881 to the same extent as drinking produced by angiotensin I infusion.5 Angiotensin I plasma levels were determined after angiotensin I infusion and isoprenaline application. Both were significantly raised by SQ 20881.6 It is concluded that one of the mechanisms which mediate the dipsogenic effect of isoprenaline is stimulation of the renin-angiotensin system and that increased plasma levels of angiotensin I may play a substantial role in this type of drinking.


Calcium efflux and secretion of alpha-amylase from rat pancreas. 1 The efflux of (45)Ca from rat pancreas was investigated in relation to the secretion of alpha-amylase.2 The (45)Ca space in the pancreas was increased 1.6 times when pancreatic tissue was incubated in media in which the extracellular calcium concentration was reduced from 1.0 to 0.05 mM.3 Carbachol increased the rate of (45)Ca efflux from the tissue and this effect was associated with an increase in the release of alpha-amylase. Dibutyryl cyclic adenosine monophosphate (AMP) also evoked a secretory response, but did not alter the rate of (45)Ca efflux. In combination with carbachol, the dibutyryl analogue of cyclic AMP reduced the carbachol-stimulated increase in (45)Ca efflux while enhancing the release of secretory protein.4 The stimulatory effect of carbachol on (45)Ca efflux was observed when pancreatic tissues were incubated in media containing a high concentration of ethyleneglycolbis (beta-aminoethyl)-N,N'-tetraacetic acid (EGTA).5 Atropine blocked the effects of carbachol on both (45)Ca efflux and secretion of alpha-amylase.6 It was concluded that intracellular calcium can and may sustain stimulus-secretion coupling in the rat exocrine pancreas.


Proceedings: Catecholaminergic control of thyroid stimulating hormone (TSH) and adrenocroticotrophic hormone (ACTH) secretion.


Effects of prostaglandin inhibitors on angiotensin, oxytocin and prostaglandin F2 alpha contractile effects on the rat uterus during the oestrous cycle. 1 In the rat isolated uterus maximal spontaneous contractions and maximal sensitivity to angiotensin II, oxytocin and prostaglandin F(2alpha) were observed in di- and proestrus. Minimal sensitivity to the three agonists was observed in metoestrus. Maximal contractile effects of angiotensin II, oxytocin and prostaglandin F(2alpha) were thus observed when the ratio oestrogen/progesterone levels was high.2 The oestrogen-dependent sensitivity of the rat uterus is partially mediated by endogenous prostaglandins. Indomethacin suppressed the increased sensitivity to angiotensin and oxytocin present in dioestrus and proestrus but did not affect that to prostaglandin F(2alpha). Polyphloretin phosphate at a concentration of 10 mug/ml resulted in complete identity of dioestrus and metoestrus dose-response curves to angiotensin and oxytocin.3 Spontaneous uterine contractions observed when oestrogen levels are high are also dependent on intramural prostaglandins as they were inhibited by indomethacin and polyphloretin phosphate. In the metoestrus uterus, prostaglandin F(2alpha) induced the reappearance of spontaneous contractions.4 Prostaglandin F(2alpha) had a potentiating effect on angiotensin-elicited contractions which persisted after washing out prostaglandin F(2alpha).


Proceedings: Modulation of frequency-dependent noradrenaline release by calcium, angiotensin and morphine.


Proceedings: The pharmacological effects of imidazole and some of its derivatives on neuromuscular transmission.


Effect of 6-hydroxydopamine on hypothalamic norepinephrine and dopamine content, ultrastructure of the median eminence, and plasma corticosterone.


Processing Marek's disease vaccine.

Processing Marek's disease vaccine.


Growth dependent 2-deoxy-D-glucose uptake in transformed and normal mouse cells.


The pineal gland of the mole (Talpa europaea L.) I. The fine structure of the pinealocytes.


On the morphology of the human Sertoli cell.


Ultrastructure of the nerve plexus in flatworms. I. Peripheral organization.


Chicken pars distalis development.  I. Light and electron microscopy of the anlage at Hamburger and Hamilton's stages 17, 20, 24, 27; days 2.6, 3, 4, 5 of incubation.


Ultrastructural changes in the tail muscles of anuran tadpoles during metamorphosis.


Fat absorption by the enterocytes of the carp (Cyprinus carpio L.).


Pigment cell differentiation in albino goldfish. I. Ultrastructural differentiation of retinal pigment epithelium.


The onset of differentiation in the epiblast of the chick blastoderm (SEM and TEM).


In vivo uptake of horseradish peroxidase by skin receptors of the duck.


Comparison of the immune response of the rabbit to virulent and attenuated strains of type 1 poliovirus. Rabbits were immunized with the attenuated LSc, 2a(1)b or the virulent Mahoney strain of type 1 poliovirus, or with a partially purified preparation of the latter. It was found that the animals responded in a heterogeneous manner to antigens with a homogeneous virion population, such as LSc,2a(1)b and, to a lesser extent, the partially purified Mahoney antigen. This heterogeneous immune response permits the preparation of specific antisera for intratypic serodifferentiation tests. However, the unpurified Mahoney strain, which has a heterogeneous virion population, induced a homogeneous immune response; all the antisera had a wide spectrum of antibodies and could not distinguish the wild from the attenuated strain. The differentiation capacity of the specific antisera was found to be linked to either the IgM or the IgG. The possible implications of these findings for human vaccination against poliomyelitis are discussed.


Alpha feto-protein in the diagnosis of primary hepatocarcinoma in Salisbury.


Presence of poliovirus and other enteroviruses in sewage. A survey in Czechoslovakia, 1969-72. Samples of sewage, collected from 43 nurseries and 22 main town sewers in Czechoslovakia, showed the presence of polioviruses 6-7 months after vaccination with live oral poliovaccines. The presence of other enteroviruses in sewage followed their known prevalence in the community during the summer and autumn.


Scope and limitations of a kinetic assay for serum 5'-nucleotidase activity.


A modified method for phenotyping of hyperlipoproteinaemia on agarose electrophoresis.


The metabolic transformation of phenylbutazone in the alloxan diabetic rat.


Multiple sclerosis with impaired neuromuscular transmission.


Plasma renin activity of rats and rabbits immunized against angiotensin II.


Pituitary acidophils with small or medium-sized granules alone in normal and adrenalectomized rats with special reference to possible ACTH secretion.


Hepatocellular carcinoma in pregnancy.

Hepatocellular carcinoma in pregnancy.


Phosphaturic effect of I.V. Administered calcitonin in man.


Carbohydrate metabolism in aflatoxin B-1 toxicity.


Two unusual cases simulating hepatoma.

Two unusual cases simulating hepatoma.


Antagonism of insulin and lipolytic hormones in the control of adenylate-cyclase activity in fat cells,.


Enzyme polymorphism in rat-liver microsomes and plasma membranes. 1. An immunochemical study of multienzyme complexes and other enzyme-active antigens.


Utilization by yeast of D-lactate and L-lactate as sources of energy in the presence of antimycin A.


Effects of catecholamines, cyclic nucleotides and phosphodiesterase inhibitors on contractions of skeletal muscles in anaesthetized cats.


Enzyme polymorphism in rat-liver microsomes and plasma membranes. 2. An immunochemical comparison of enzyme-active antigens solubilized by detergents, papain or phospholipases.


The ACTH-secreting system in hyperthyroid patients: chrono-regulation and dynamic behavior.


Binding of salicylate in the adenosine-binding pocket of dehydrogenases.


Presence of cytochrome c1 in cytoplasmic "petite" mutants of Saccharomyces cerevisiae.


Processing and release of ribosomal RNA from isolated nuclei: analysis of the ATP-dependence and cytosol-dependence.


A novel sequence in kangaroo angiotensin I.


Nodular tenosynovitis. Benign giant cell synovioma.


Tumours and hormonal changes produced in rats by subcutaneous injections of linoletic acid hydroperoxide.


Peptide alkaloids.

Peptide alkaloids.


Stereospecificity of murine myeloma protein-315 to enantiomeric spin labeled dinitrophenyl hapten.


Dual susceptibility of A 3T3 mouse cell line to infection by N- and B-tropic murine leukemia virus: apparent lack of expression of the FV-1 gene.


Cell relationships in transformation of human leukocytes by Epstein-Barr virus.


Enhancing effect of green monkey kidney cell extract on the transformation of mouse cells by SV40.


Effects of dibutyryl cyclic adenosine 3':5' monophosphate and theophylline on 2-deoxy-D-glucose and 2-aminoisobutyric acid uptake by hamster embryo cells.


Segregation of the EBV-determined nuclear antigen (EBNA) in somatic cell hybrids derived from the fusion of a mouse fibroblast and a human Burkitt lymphoma line.


Studies on a gross-virus-induced lymphoma in the rat. I. The cell-mediated immune response.


Alteration of in vitro anti-tumor activity of tumor-bearer sera by absorption with Staphylococcus aureus, Cowan I.


Cell-surface antigens of murine sarcoma-virus-transformed non-producer cells: further evidence for lack of transplantation immunity.


The mechanism of inhibition by Corynebacterium parvum of the growth of lung nodules from intravenously injected tumour cells.


Cytogenetic studies in permissive and abortive infections by adenovirus type 12.


Herpesvirus ateles: transformation in vitro of marmoset splenic lymphocytes.


Cellular immunity against tumor-associated antigens in humans: lymphocyte stimulation and skin reaction.


Influence of interferon on virus-particle synthesis in oncornavirus-carrier lines. II. Evidence for a direct effect on particle release.


Early events in transformation of human cord leukobytes by Epstain-Barr virus: induction of DNA synthesis, mitosis and the virus-associated nuclear antigen synthesis.


Synthesis of a DNA binding protein in normal and transformed cells.


Immune responses of adenovirus type-9 infected (tumor-free) male rats against adenovirus type-9 induced mammary fibroadenomas and a chemically induced mammary carcinoma.


Cytostasis of lymphoma cells in suspension: probable non-thymic origin of the cytostatic lymphoid cells in mice bearing mys -induced tumors.


Cell-mediated anti-tumor immunity in oncornavirus-induced tumors: specific cytostasis of tumor cells by spleen amd lymph-node cells.


Embryonic antigens associated with chemically induced colon carcinomas in rats.


Induction of polyoma tumors in athymic nude mice.


Effect of the Fv-1 gene on leukemia virus in mouse cell heterokaryons.


Flow microfluorometry analysis of DNA content in Chinese hamster cells following infection with simian virus 40.


Cytotoxic antibodies to cultured lymphoblasts unrelated to Epstein-Barr virus.


Virus--host-cell relationship of Herpesvirus sylvilagus with cottontail rabbit leukocytes.


Tumour-specific antibody neutralization of factors in rat hepatoma-bearer serum which abrogate lymph-node-cell cytotoxicity.


Tumor metastasis in mice with reduced immune reactivity.  II. Studies with a highly antigenic MCA-induced sarcoma in thymectomized and/or sub-lethally irradiated C57BL/6J mice.


Cell interactions in adoptive immune rejection of a syngeneic tumor.


Absence of RSV production in enucleated RSV-transformed virogenic Mammalian cells fused with chick embryo fibroblasts.


Distribution of the blood group antigens a and b on human spermatozoa. Blood group antigens A and B was demonstrated on human spermatozoa by a) conventional mixed agglutination test, ((b), immunofluorescense, and c) mixed agglutination performed with a mixture of fluorescein labelled and unlabelled erythrocytes as indicator cells for A or B specificity by fluorescence microscopy. Spermatozoa obtained from individuals with the blood group AB and who were secretors showed surface antigens with either A or with B specificity. The results by indirect immunofluorescence were in accordance with those of the two kinds of mixed agglutination. About 30-35% of spermatozoa showed fluorescence when the antibodies were directed against only one of the antigens of the heterozygous donor. With a mixture of antibodies corresponding to the two genes of the donor the percentage of the fluorescent spermatozoa was consistantly higher, about 50-55%. However, with a mixture of antiserum, no complete sum of the percentages was observed, compared with the monospecific antisera. Unreacted, "neutral" spermatozoa were always present in the samples. The limitations of the applied methods for a quantitative detection of antigens were discussed,


Effect of Lh-Rh on antibody formation, sperm count and plasma levels of gonadotropins and testosterone in normal men. Synthetic LH-RH was given to 15 healthy men divided into 3 groups of 5 each. A dose of 250 mug LH-RH was injected as a single intravenous bolus for  1 day, every other day for 3 days or for 7 consecutive days respectively. Antibody formation against this synthetic hormone was measured by testing for binding with 125I labeled LH-RH. No antibody formation was detected in any group during the seven day period. Sperm counts were done 1 week before and immediately after the administration of LH-RH, as well as 3 months later. Changes in sperm counts could not be related to the number of injections received. Gonadotropins (FSH and LH) were measured by RIA. There was a significant rise in plasma levels of both gonadotropins, but no signigicant changes in their basal level or percent response resulted from the repetitive administration of LH-RH. Plasma testosterone was also measured by RIA. There was no significant channge in either baseline or percent response in any of the groups after 1, 3 or 7 injections. Some individuals did show  a significant rise in plasma testosterone levels, but this was not consistent and was not related to the number of injections administered. Thus, in this study, the repetitive administration of LH-RH did not alter pituitary responsiveness or result in antibody formation.


Binding of steroids to blastokinin.

Binding of steroids to blastokinin. The authors studied the binding of steroids with Blastokinin (BKN). They confirm that progesterone is the steroid that has a greater affinity for BKN than estradiol or testosterone; the latter is hardly bound to the protein at all. They also show that the presence of estradiol interferes with the formation phase of the BKN-progesterone complex, while testosterone does not. When the BKN-progesterone complex is already formed, a release of the progesterone occurs only when there is a double concentration of estradiol. The biological importance of this phenomenon is briefly discussed. The binding capacity of blastokinin (BKN) to progesterone, estradiol, and testosterone was studied in vitro in uterine secretions collected from rabbit uteri.  Progesterone was found to bind to BKN in greater quantities than estradiol, while testosterone exhibited virtually no affinity to BKN.  Study with radioactive steroids indicated that estradiol, in equimolar concentrations, caused a considerable reduction in progesterone binding to BKN, while testosterone had no effect on progesterone-binding.   However, when the progesterone-BKN complex was performed, twice the concentration of labeled-estradiol was required to cause a decrease in progesterone radioactivity linked to BKN.  The effect was not progressive, and appeared to function on a threshold principle.


Increased potency of four analogues of LH-releasing hormone in man. Four analogues of LH-releasing hormone (LH-RH) were injected iv. in normal men at a dose of 25 mug: des-Gly-10NH2-LH-RH ethylamide (9-EA-LH-RH); des-Gly-10-NH2-LH-RH propylamide (9-PA-LH-PH), d-ala-6-LH-RH; and D-Ala-6, des Gly10-NH2-LH-RH ethylamide (D-Ala-6,9-EA-LH-RH). Each peptide tested was found to be more potent than LH-RH in releasing LH and, to a lesser extent, FSH. The times of the peak values of LH after the analogues were similar to LH-RH (about 20 minutes). However, LH as well as FSH levels were considerably delayed in returning to baseline after administration of D-Ala-6,9-EA-LH-RH. The half-time disappearances of the 9-EA and 9-PA analogues could be tested and were found to be similar to that of LH-RH. The increased potency and prolonged duration of action of D-Ala-6,9-EA-LH-RH suggest the potential usefulness of this compound in stimulating ovulation and perhaps spermatogenesis. Luteinizing hormone-releasing hormone (LH-RH), and 4 of its analogues were each tested in groups of 6 normal men.  25 mcg of des-Gly-10-NH2-LH-RH ethylamide (9-EA-LH-RH), des-Gly-10-NH2-LH-RH propylamide (9-PA-LH-RH), D-Ala-6-LH-RH, D-Ala-6, des Gly-10-nh2-lh-rh ethylamide (D-Ala-6,9-EA-LH-RH), and LH-RH were injected iv and the halftime disappearance  from the blood of 2 of these analogues as well as LH and follicle stimulating hormone (FSH) levels were determined by radioimmunoassay.  The peptides tested were found to be more potent than LH-RH in releasing LH and, to a lesser extent, FSH.  The time of the peak values of LH and the analogues was about 20 minutes, however, LH and FSH were delayed in returning to baseline after D-Ala-6,9-EA-LH-RH.  9-EA and 9-PA analogues were similar to LH-RH in halftime disappearances.  D-Ala-6,9EA-LH-RH showed increased potency and prolonged duration of action, thus suggesting potential usefulness in stimulating ovulation and perhaps spermatogenesis.


Response to LH-RH in men infused with theophylline. In an attempt to ascertain certain aspects of the effects of cyclic AMP on the release of LH and FSH in the human being, ten normal men were tested with LH-RH during the infusion of theophylline, a methyl xanthine derivative which inhibits the inactivation of cyclic AMP by phosphodiesterase. The results were compared with the gonadotropin values obtained when LH-RH was injected into the same subjects without theophylline. Although no consistent effect of theophylline was found on either the basal levels of LH and FSH or the magnitude of their increase after LH-RH, an action of cyclic AMP on gonadotropin release is not excluded.


Isolation and characterization of two antigens from human seminal plasma. A simple method is described for the isolation of two glycopeptides from human seminal plasma. One of them cross-reacts with human spermatozoa, a fact which will help the study of antigenicity of sperm cells. The glycopeptides obtained (G1 and G2) are homogenous, as judged by disc electrophoresis, ultracentrifugation, immunodiffusion and immunoelectrophoresis. The weight average molecular weight of both glycopeptides was about 10 000 for both fractions. Differences in the amino-acid and carbohydrate composition of both glycopeptides were observed. The content of histidine was higher in one of them (G1). Only slight differences in the content of sialic acid of both glycopeptides were observed.


Ultrastructure of human sperm acrosome and determination of acrosin activity under conditions of semen preservation. Electron microscopical studies of cryopreserved human spermatozoa show considerable ultrastructural changes of the acrosome in spite of the use of a cryoprotective agent (10% glycerol), as compared with fresh semen and with glycerolated semen before freezing. A nearly complete loss of the plasma membrane and a partial removal of the outer acrosomal membrane with depletion of the acrosomal content is observed whereas the inner acrosomal membrane and the equatorial segment remain intact. Simultaneous determinations of the activity of the proteolytic penetration enzyme acrosin (EC 3.4.21.10) before and after addition of glycerol and immediately after freezing and thawing reveal a significant increase of the extractable acrosin activity in glycerol-treated and in cryopreserved spermatozoa compared with the fresh material. The results indicate an association of the acrosin with the inner acrosomal membrane and/or the equatorial segment.


Effect of oral contraceptives on Serum magnesium levels. Serum magnesium levels were determined by atomic absorption apectrophotometry in 224 Nigerian women attending the post-partem Family Planning Clinic of University College Hospital, Ibadan. The concentrations of serum magnesium in 44 control subjects were similar to those of 87 women who had been fitted with the Lippes loop. Serum magnesium levels were significantly lower (P smaller than 0.001) in 28 women taking Ovral 28 and in 65 women taking Norlestrin Fe in comparison with the control group. Considering the possibility that magnesium may be involved in the development of thromboembolism in humans, serum magnesium determinations were done in a group of 224 women attending the Post-Partum Family Planning Clinic of University  College Hospital in Ibadan, Nigeria.  Of these, 44 had been given no therapy, 87 had been fitted with Lippes loops, 28 were taking Ovral 28, and 65 were taking Norlestrin Fe.  Those taking Ovral 28 had done so for an average of 9 months and those taking Norlestrin Fe, for 6 months.  Magnesium concentrations were determined by atomic absorption spectrophotometry on serum deproteinized by a lanthanum-trichloroacetic acid diluent.  Those fitted with Lippes loops had concentrations similar to the control group.  Those taking the oral contraceptives had significantly lower levels (p less than .001) than the control group.  Type of oral contraceptive or length of use was not correlated with serum magnesium levels.  Results gave lower values than reported by others.  In theory, the reduction in serum magnesium in women taking oral contraceptives would be associated with decreased clotting time and increased frequency of thromboembolic episodes.


Use of sonar in diagnosis and management of invasive gestational trophoblastic tumors. Use of sonar in differential diagnosis between normal and molar pregnancies is well established. In this study, invasive trophoblastic tumors were investigated. Intrauterine masses were demonstrated sonographically in all 4 cases, As well as H.C.G. assay and chest X-ray, sonographic follow-up also seems to be of value in evaluating the effectiveness of the chemotherapy.


Observations on patients two years after legal abortion,. The patients who underwent legal abortions in 1971 were invited to a follow-up examination 2 years later. 25% of the 562 patients invited came to be examined. Anamnestic data were obtained on 60%. Early complications had occurred in 10% of the cases. During the 2-year period which had elapsed, the 338 patients had had 73 new pregnancies (22%), of which 60% had terminated in delivery. No spontaneous abortions in the second trimester had occurred in the new pregnancies. 15% of the patients had resorted to repeated legal abortion. 44% of the patients were using oral contraceptives or IUCD 2 years after the abortion. A gynecological examination gave rise to a suspicion of cervical insufficiency in 10% of the cases examined. Hysterosalpingography suggested tubal pathology in 18%. In laparoscopy no occurrences of bilateral tubal occlusion were found. The need of new follow-up examination later is obvious.


Influence of vitamin C nutriture and inanition on ACTH stimulated release of adrenal corticosteroids in guinea pigs.


Inactivation of mouse Elberfeld virus by periodate.


The interference by Japanese encephalitis virus with Newcastle disease virus in Vero cells.


C-type particles in cultured lymphocytes from highly leukemic cattle.


Equine herpesvirus 1: biological and biophysical comparison of two viruses from different clinical entities.


Increased DNA-dependent RNA polymerase activity from CV-1 cells productively infected by SV40.


The chromosomes of primary 7,12-dimethylbenz(a)anthracene-induced rat sarcomas.


Adrenergic neurotransmitter storage and release.


The effect of parinfluenza virus type 3 on newborn ferrets.


Use of frozen chick and duck embryo cells for plaque assays of arboviruses and rickettsiae.


Cosmetics in search of a definition.

Cosmetics in search of a definition.


Cosmetic legislation: benefit-risk.

Cosmetic legislation: benefit-risk.


Cosmetics: a view from the bridge.

Cosmetics: a view from the bridge.


Cosmetic legislation: technical consideration.


Antibodies against Philebotomus fever viruses in human population of Kuwait.


"A study of a case of acute paralytic poliomyelites".


Maintenance of the native conformation in liver fructose 1,6-bisphosphatase following modification of its primary structure.


Degradation of rabbit liver fructose 1,6-bisphophatase by lysosomal proteinases.


Changes in sleep cycle patterns with age.


Aleutian disease in mink: virology, immunology and pathogenesis.


The stimulation of chloride transport by prostaglandins and their interaction with epinephrine, theophylline, and cyclic AMP in the corneal epithelium.


Statistical estimation of parameters in a phylogenetic tree using a dynamic model of the substitutional process.


Quantum mechanical calculations of P--C and P--H spin-spin coupling constants in model compounds and in nucleotides.


Enzymatic reactions involved in streptomycin biosynthesis and metabolism.


Preparations of deuterated proteins and enzymes.


Nuclear relaxation measurements of water protons and other ligands.


A biological agent isolated by transformation.


Glomus tympanicum tumors: early diagnosis by arteriography.


Cancer of the breast in twin sisters.

Cancer of the breast in twin sisters.


Primary liver cancer and drug therapy.

Primary liver cancer and drug therapy.


Characteristics of Paget's carcinoma of the nipple and problems of its negligency. The analysis of 73 cases revealed Paget's disease of the nipple, known as a precancerous state, to be a special form of the intraductal mammary cancers. In the majority of the cases, apart from a superficial exulceration malignant transformation in the glandular substance is present. Thus, the treatment schedules have to be similar to those of other mammary cancers; in cases localized to the nipple, simple mastectomy with axillary block dissection, is suggested; in cases with tumors of the glandular substance, radical mastectomy according to Halsted is suggested. The frequent negligency is partly a result of an earlier attitude of the physicians being too conservative with therapeutic procedures.


Metabolism of acid mucopolysaccharides in hepatoma and in normal liver. The metabolism of acid mucopolysaccharides in Novikoff hepatoma and in hepatoma BW7756 was studied using the precursors -35S-sulfate, -H-glucosamine and -14C-galactosamine. There is an active formation of sulfated mucopolysaccharides at the mitochondria and cell membrane level. Hepatoma cells synthesize heparin to a lower rate than liver cells. In ascitic as well as in solid tumors there is a considerable accumulation of hyaluronic acid which does not seem to be elicited by the tumor cells but by the surrounding tissues. The possible implication of the low heparin production in the cell membrane characteristics is discussed.


Studies on the effect of oral hypoglycaemic agents on hepatic glycogenolysis.


Stimulation of cyclic AMP formation in the anterior pituitary by 11-deoxyprostaglandins and their analogues.


Synthesis and properties of poly (1,N-6-ethenoadenylic acid) and poly (3,N-4-ethenocytidylic acid).


Phosphorylation of the wedge-presumed initiator of DNA replication by cyclic AMP dependent protein kinase.


Thermostable glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus: II. Kinetic and immunokinetic analysis.


Preliminary characterization of minor bases from DNA fractionation, with discussion on 5-chlorocytosine as artifact or naturally occurring base.


Circadian rhythm and basal ACTH secretion after CNS radiofrequency current lesions.


Cyclic adenosine 3',5'-monophosphate in prostaglandin-induced abortion. Cyclic AMP (cAMP) in blood samples was followed during abortions induced with an intraamniotic injection of prostaglandin F2a (PGF2a) and E2 (PGE2) in women in the second trimester.  The effect of intraamniotic administration of PGE2 on tissue cAMP levels and adenyl cyclase (AC) and cyclic nucleotide phosphodiesterase (PDE) in myometrium, maternal abdominal rectus muscle, placenta, and fetal liver and leg muscle was studied in women undergoing abortion with hysterotomy.  Saline was injected intraamniotically into controls.  Plasma cAMP values showed inconsistent variation after injection of both types of PGs.  On administration of PGE2 uterine contraction started after 3 minutes.  Myometrial cAMP increased 4-fold within 10 minutes, and these values also showed inconsistent variation.  Neither AC or PDE activities were affected in myometrium by PGE2.


Factors affecting the activity of glycogen synthase D phosphatase from rat liver in vitro.


Formation of N-acetylglucosamine 6-phosphate from UDP-N-acetylglucosamine in rat tissues.


Cochleography and recruitment.

Cochleography and recruitment.


Adverse effects of antibiotics.

Adverse effects of antibiotics.


Biological systems analysis and evoked potentials of the brain.


TSH stimulation of diiodotyrosine--aminotransaminase in the rat thyroid.


An inhibitor of the toad bladder cyclic AMP system and theophylline-stimulated Na+ transport.


A case of omphalocele with absence of the thumb and syndactily.


The use of vaccines in respiratory virus infections;.


Cancer and infectious diseases.

Cancer and infectious diseases.


Acute virus hepatitis.

Acute virus hepatitis.


Acute virus hepatitis: immunology.

Acute virus hepatitis: immunology.


Ultrastructure of a vaginal myxoma of a rat.


Mobilization and release of cationic protein B2 from rabbit PMN-leukocytes.


Intraepithelial vibrio associated with acute typhlitis of young rabbits.


Carcinoid tumor in the small intestine of a dog.


The role of parainfluenza viruses in respiratory disease in Trinidad.


The effects of adrenalectomy and hydrocortisone replacement on the thyroid of the adult male rat.


Preparation and study of a crystalline hyperglycemic and diabetogenic substance in urine.


Various forms of specialization in cells which synthesize immunoglobulins.


Molecular basis of the biological function of molybdenum. Developmental patterns of sulfite oxidase and xanthine oxidase in the rat.


Chemotherapeutics with tuberculostatic action.


Epidermal growth factor. Characteristics of specific binding in membranes from liver, placenta, and other target tissues.


Autophosphorylation of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain.


Deoxyribokinase from Salmonella typhimurium. Purification and properties.


A study of adenosine 3':5'-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase.


Inhibition of vitamin D metabolism by ethane-1-hydroxyl-1, 1-diphosphonate.


Specificity for the glucose-6-P inhibition site of hexokinase.


Transformation of neutral to alkaline fructose 1,6-bisphosphatase. Converting enzyme activity in the large-particle fraction from rabbit liver.


Two-dimensional acrylamide gel electrophoresis of cancer-patient serum proteins. Sera from normal volunteers, patients with a variety of non-neoplastic diseases and patients with malignant or benign tumors were examined by two-dimensional acrylamide gel electrophoresis. In this technique the serum is first separated in an acrylamide gel column followed by a second electrophoresis at right angles to the first separation in a continuous concave 2 to 30 percent gradient acrylamide gel slab. The stained two-dimensional gel slab appears as a "fingerprint" pattern or "map" of the separated serum proteins. Both qualitative and quantitative differences in the fingerprint patterns of cancer-patient sera were observed. The quantitative alterations did not appear specifically associated with malignant tumors. However, several qualitative differences were detected, of which some may represent markers of malignancy as they were not observed in the normal, abnormal and benign tumors control sera. At least one of the abnormal protein stained spots, a prealbumin, appears to be restricted and related in some way to cancer of the lymphoreticular system. These data, although limited, support earlier indications that two-dimensional acrylamide gel electrophoresis offers a new and powerful tool to the cancer scientist for the detection of alterations in cancer-patient sera. A discussion of its possible use to examine other biological fluid and tumor extracts from the cancer patient is presented.


Specific inhibition of a primer-dependent nucleotide chain elongation reaction by the rifamycin derivative AF/013.


Human chorionic gonadotropin as a tumor marker. Ectopic production and secretion of hormones by a wide variety of tumors has been known for decades. Initially, ectopic hormone secretion was recognized by signs and symptoms of excess circulating biologically active hormone. With development of more sophisticated assay techniques, biologically inactive fragments and "big" forms of authentic hormones have been identified in these syndromes. Recently, a specific hCG assay has been developed for selectively measuring hCG in plasma samples containing both hLH and hCG. Studies reported to date suggest that hCG may be a good tumor marker. Using that assay system, several hundred sera obtained from patients with a wide variety of tumors were screened. Patients with tumors of the stomach, liver, ovary and testis were found to have the highest incidence of ectopic hCG secretion.


Peroxide oxidation of Tris to a free radical.


The involvement of calcium and angiotensin II in vascular reactivity to noradrenaline in renal/salt hypertension.


Ketamine as a suitable anesthetic agent for the measurement of renal clearance in the rat.


Effect of Asp1-beta-amide-Val5-angiotensin II, Sar1-lle5-angiotensin II and (N,N-dimethyl) Gly1-lle5-angiotensin II on isolated perfused rabbit kidney.


Adenoid cystic carcinoma of the head and neck: a report of 30 cases.


Tumours of the deep lobe of the parotid.


Ovarian contractility.

Ovarian contractility.


A Possible regulatory role of mitochondrial calcium uptake in uterine contractions.


Cyclic AmP and oviduct contractility.

Cyclic AmP and oviduct contractility.


The corpus luteum studies in vitro.

The corpus luteum studies in vitro.


Cyclic AMP and genetic sterility of a male restricted (Hre/plus) mutant of Rattus.


Parturition in the sheep and the human.

Parturition in the sheep and the human.


Adenyl cyclase of oxyntic cells. Its association with different cellular membranes.


The location of spin probes in two phase mixed lipid systems.


Effect of exogenous lysolecithin on liposomal membranes. Its relation to membrane fluidity.


An interacting spin label study of lateral expansion in dipalmitoyllecithin-cholesterol bilayers.


Cellular uptake of cyclic AMP captured within phospholipid vesicles and effect on cell-growth behaviour.


Wilms' tumor in father and son.

Wilms' tumor in father and son.


Clinical and familial studies in the Noonan syndrome.


Reproductive genetics.

Reproductive genetics.


Possible linkage between the loci for the trichodentoosseous (TDO) syndrome and the ABO blood group system:  genetic and clinical implications.


A generalized disorders of the connective tissues with progeria, choanal atresia, symphalangism, hypoplasia of dentine and craniodiaphyseal hypostosis.


Cytochemical localization of glucose 6-phosphatase in ciliated cells of rat tracheal epithelium.


Urinary cyclic AMP in the switch process from depression to mania.


Pseudomonas aeruginosa infections.

Pseudomonas aeruginosa infections.


Biological properties of "baby Taiwan monkey" strains of human adenovirus type 12.


Non-interaction between in vivo and cell free nuclear binding of estrogen receptor.


The effect of alpha-melanocyte stimulating hormone on aldosterone production in the rat.


Estrogen specificity of the induction of lipovitellin syntheses and evidence for a specific estrogen-binding component.


The effect of adrenal denervation on ACTH-induced hypertension in sheep.


Disappearance rate of circulating renin after bilateral nephrectomy in the rat.


Androgen receptors from the epididymis of the rhesus monkey. The demonstration and partial characterization of androgen "receptors" in the epididymis of the rhesus monkey are reported.  The affinity of specific binding sites for dihydrotestosterone was higher than that for testosterone while cortisol and corticosterone did not compete.  Density gradient analysis of the cytosol revealed that macromolecules responsible for specific binding have a sedimentation coefficient of 8 S.  The isoelectric point of the complex was 5.5 and thermoability was evidenced by the release of hormone during incubation at 27 degrees C (20% and 44% after 30 and 60 minutes, respectively.)  A concomitant decrease in radioactivity bound to 8  S cytoplasmic components suggests the translocation into the nuclei of a modified 8 S-receptor complex.  These data indicate the presence of similar cytoplasmic and nuclear androgen receptors in the monkey seminal vesicles and prostate gland.


The ultrastructural demonstration of virus-like particles in human leukaemic cells.


Testicular and prostatic cyclic amp metabolism following chronic cadmium treatment and subsequent withdrawal.


Interpretation of some properites of A-V conduction with the help of analog simulation.


A comparison of the phosphofructokinase isoenzyme profiles of transplantable hepatomas and normal liver.


Response of mice selected for rapid post-weaning growth rate to dietary manipulation.


The effects of growth and endurance training on the cytochrome C content of rat soleus, plantaris and gastrocnemius muscles.


Fetal lung maturation.

Fetal lung maturation.


Acute disseminated intravascular coagulation as the presenting and predominant clinical manifestation in a patient with a mucinous adenocarcinoma. A patient with a fulminant clinical course of disseminated intravascular coagulation (DIC) is described. The DIC was first manifested by neurological symptoms and later dominated by hemorrhagic phenomena. Bleeding manifestations were not controlled by treatment with heparin, later supplemented with epsilon-amino-caproic acid and fresh blood. The patient's condition precluded the performance of extensive diagnostic procedures. On the assumption that malignancy was the underlying disease, cyclophosphamide and hydrocortisone were administered. In spite of intensive treatment the patient died. Autopsy revealed mesenteric lymph node metastases of a mucinous adenocarcinoma, probably originating in the colon. It is emphasized that in some patients  with malignant disease, acute DIC is the presenting and the predominant symptom. In such patients the hemorrhagic manifestations may not be controlled unless the underlying neoplastic disease is detected and treated.


Proceedings: An enzymatic method for isolation of tubules and cells from human kidney cortex.


Proceedings: ADP synthesis by oxidation of a copper(II)-thiol complex with oxygen.


Proceedings: Steroid hormone receptors in rat testicular tissue.


Proceedings: Properties of specific estrogen-binding components in immature rat kidney.


Proceedings: Activation and induction of microsomal UDPglucuronosyltransferases in rat liver and Morris hepatomas.


Proceedings: Binding of the glucocorticoid receptor of liver cytosol to DNA.


Proceedings: Preparation and partial chemical analysis of milk-fat-globule membrane and mammary tumour virus obtained from Swiss albino mice.


Proceedings: A possible function of phosphatidylinositol turnover.


[Electrophysiology of involuntary or smooth muscle].


Proceedings: Properties and nature of a lysosomal activator of the proenzyme of bone collagenase.


Proceedings: Common control by a single gene of the syntheses of cytochrome oxidase and oligomycin-sensitive ATPase.


Proceedings: Defective nuclear binding of a steroid hormone receptor.


Proceedings: Different intramembranous location of two microsomal enzymes.


Proceedings: Alterations of the level of cyclic AMP in mouse epidermis after stimulation with tumor-promoting phorbol esters.


Proceedings: Phosphatase activity in the hepatopancreas of the crayfish Astacus leptodactylus.


Proceedings: In vitro test for human chorionic gonadotropin.


Proceedings: Substituted cAMPs: conformation and interaction with specific enzymes.