Interchangeability of phosphorylation coupling factors in photosynthetic and respiratory energy conversion. The nonsulfur purple photosynthetic bacterium Rhodopseudomonas capsulata can obtain energy for growth either by anaerobic photophosphorylation or dark oxidative (aerobic) phosphorylation. Successful resolution of phosphorylation coupling factors from energy-converting membranes of this bacterium permitted tests for reciprocal function of such protein factors in oxidative-and photophosphorylation processes. Evidence was obtained for the interchangeability of coupling factor preparations from dark-grown and photosynthetically grown cells in both kinds of energy conversion.


Fatty acid biosynthesis in aorta and heart.


The origin of hydrogen in fatty acid synthesis.


Of nurses and physician's assistants.

Of nurses and physician's assistants.


Diagnosis of Zollinger-Ellison syndrome. Ulcerogenic tumor of the pancreas. A small proportion of patients with peptic ulcer disease harbor a hormone-secreting pancreatic tumor as the cause of the ulcer diathesis, and if this is not recognized, they may suffer undue morbidity and mortality. Close attention to the patient's history, roentgenographic findings and gastric secretory studies will allow identification of ulcerogenic tumors of the pancreas. Other clues to the diagnosis include associated endocrine abnormalities and diarrhea.


Neurosecretory cells and their axon pathways in Culiseta inornata (Williston) (Diptera: Culicidae).


Susceptibility of erythrocytes to phagocytosis after exposure to physiological saline.


The effect of pre-inhibition of DNA synthesis in continuous human amniotic cell culture (strain FL) on adenovirus type 5 reproduction.


Zonal electrophoresis of pseudorabies virus in sucrose density gradient.


Pathogenicity of cryophilic and thermophilic strains of influenza type A2 virus for developing chick embryos and albino mice.


Sialidase activity of chick and mouse embryo tissue culture cells infected with myxoviruses and the effect of actinomycin D on this activity.


Release of interferon by mouse peritoneal cells in vitro. The requirement of contact with endotoxin and the temperature dependence of release.


Relation of incubation temperature to interferon production in mouse peritoneal cells infected with Newcastle disease virus.


Chromosomal aberrations in human amniotic cells before the completion of a single growth cycle of measles vaccine virus L-16.


Studies on the pathogenesis of Aujeszky's disease. 3. The distribution of virulent virus in piglets after intranasal infection.


Propagation of tick-borne encephalitis virus variants in short-term cultures of normal human leukocytes.


Subcutaneous and inhalation infection of guinea pigs with Venezuelan equine encephalomyelitis virus.


Diagnosis of Venezuelan equine encephalomyelitis virus by immunofluorescence.


Relationship of Tribec virus to tick cells and tissues.


The effect of aerobiosis on adenovirus multiplication.


Studies on the virulence of tick-borne encephalitis virus. X. Character of attenuated HY-HK28 "2" virus during propagation in monkey brain or ixodes ricinus ticks.


Cationic stabilization of echovirus haemagglutinins.


Determination of the sensitivity of the plaque virus neutralization test: comparison with the sensitivity of antibody detection in haemagglutination and complement fixation systems.


Difference in the multiplication of phase I and II Coxiella burnetti in cell cultures.


The search for new drugs: a look from outside.


3-mercaptopropionic acid: convulsant and lethal properties compared with other sulfur-convulsants; protection therefrom.


Evaluation of the pharmacologic basis for the therapy of circulatory shock.


Comparison of the effect of propranolol, lignocaine, and other drugs on normal and raised intraocular pressure in man.


The physician's assistant: an overview.

The physician's assistant: an overview.


Muscle cholinergic dilators in the sinus baroreceptor response in cats.


Effects of bradykinin and autonomic nervous system inhibition on systemic and regional hemodynamics in the unanesthetized rhesus monkey.


Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model.


Increasing physician productivity and the hospitalization characteristics of practices using Medex. A progress report.


Observations on the structure of the small intestine in foetal, neo-natal and suckling pigs.


Hemodynamic studies with sotalol in man, performed at rest, during exercise, and during right ventricular pacing.


Parkinsonism and the hypotension caused by L-dopa.


Aortic smooth muscle responses to changes in venous return studied in intact dogs.


Alpha-adrenergic antagonism by a series of aliphatic sulfur-containing compounds.


The cardioactive and related properties of N-methyl-N-( -phenylethyl)-1,2,3,4-tetrahydro-6-methoxy-4,4-dimethyl-2-naphthylamine hydrochloride (2-AT-1).


Facilitation of the release of noradrenaline and dopamine- -hydroxylase at low stimulation frequencies by  -blocking agents.


An experimental system for the simultaneous estimation of mitostatic and lymphotoxic effects of immunosuppressants and cytostatics. Sublethally (600 R) irradiated (CBA x C57BL)F(1) mice were grafted intravenously with parental lymph node cells in doses ranging from 0.2 x 10(6) to 12 x 10(6). The transplantation of these lymphoid cells leads to inactivation of the recipient's endogenous CFU (as measured by the diminution of the number of colonies registered on the 10th day after irradiation). A 50% inactivation was observed when the graft size of the CBA cells was 0.52 x 10(6). This figure for C57BL cells was 10 times more. This experimental system evaluates two simultaneously developing processes: the multiplication of endogenous CFU and the homograft reaction of transplanted lymphocytes against them. Both processes can be quantitatively estimated simultaneously in the same experiment by the determination of the number of colonies in corresponding experimental groups. Thus it was possible in a single experiment to compare quantitatively the effect of immunosuppressants on two points: (a) mitostatic action (suppression of CFU) and (b) lymphotoxic action. The latter, a true immunosuppressive effect, represents suppression of GVH activity of lymphoid cells and is demonstrated by abolition of the inhibition of endogenous colony formation. In the present system we have tested 6-MP, ALS, cyclophosphamide, hydrocortisone, and other drugs. The definite mitostatic and lymphotoxic doses of drugs are ascertained. Cyclophosphamide and ALS proved to be drugs with high dose ranges of selective lymphotoxic action. Hydrocortisone acetate had a more narrow range of selective lymphotoxic effect. 6-MP and Imuran (azathioprine) failed to exert any selective action on lymphoid elements. They possessed pronounced mitostatic efficiency, however.


Steric aspects of adrenegic drugs. XV. Use of isomeric activity ratio as a criterion to differentiate adrenergic receptors.


Steric aspects of adrenergic drugs. XVI. Beta adrenergic receptors of guinea-pig atria and trachea.


Adrenergic blocking and cardiovascular effects of a new N-substituted tetrahydroisoquinoline (NC 7197).


Reflex and other cardiovascular changes in anesthetized dogs after beta adrenergic blockade.


The fine structural localization of norepinephrine- 3 H in the substantia nigra and area postrema of the rat. An autoradiographic study.


The effect of thiamine consumption on liver microsomal drug-metabolizing pathways.


Allergy and infection.

Allergy and infection.


Nicotinamide dinucleotides in microorganisms producing peptide and macrolide antibiotics.


Practolol and atropine medication in dental anesthesia.


Metabolic aspects of spermatogenesis.

Metabolic aspects of spermatogenesis.


The metabolism of glucose in skin maintained in tissue culture.


Histopathologic classification and natural history of malignant testis tumors in Norway, 1959-1963.


The inhibitory effect of ethanol on retinol oxidation by human liver and cattle retina.


Control mechanisms of whole number ratios of heart rate and breathing frequency.


Actinomycin-D enhancement of glutamine synthetase activity in chick embryo retinas cultured in the presence of cortisol. Cortisol can prematurely induce glutamine synthetase activity in the chick embryo retina. Under appropriate conditions, this effect can be enhanced by addition of low levels of actinomycin D; this enhancement is reversibly inhibited by cycloheximide. The magnitude of the effect is a function of time of exposure to hormone as well as antibiotic and is also a function of the age of the embryo; within the limits of the present study it did not appear to be a function of actinomycin-D concentration. The data are discussed in terms of current ideas of possible control mechanisms in animal cells.


Glutamine synthetase in the normal and dystorphic mouse retina.


Adrenergic blockade of hypocapnic cerebral arterial constriction.


Development of catecholamine--storing cells in human fetal paraganglia and adrenal medulla. A histochemical and electron microscopical study.


Hypersensitivity reactions of the heart: reduction of anaphylactic crisis by theophylline or glucagon.


Gastrointestinal complications of collagen vascular diseases. Surgical implications.


Parkinson's syndrome. Recent progress in the medical treatment and its pharmacological basis.


A light and electron microscopic study of the visual cortex of the cat and monkey.


An experimental study of the termination of the lateral geniculo-cortical pathway in the cat and monkey.


Prevalence of sub-periodic Brugia malayi in areas near the thai-Malaysian border.


Experimental studies on potential mosquito vectors of bancroftian filariasis.


Investigations on bionomics of Mansonia mosquitoes-vectors of Brugia malayi in southern Thailand.


Cell fusion and the analysis of malignancy.


Studies on sub-periodic Brugia malayi in southern Thailand.


Production of the generalized Shwartzmann reaction by activated Hageman factor and alpha-adrenergic stimulation.


Antihistaminic reactions: a review and presentation of two unusual examples.


Topical heparin in the treatment of eczema.


Effect of cromolyn sodium on childhood asthma.


Alterations in the activity of the adrenergic nervous system in heart failure.


The energy metabolism of myocardium and its regulation in animals of various age.


Myoid conduction in the siphonophore Nanomia bijuga.


Cross-validation of four MMPI scales for alcoholism.


An assay for intercellular adhesive specificity. A modification of an assay for intercellular adhesive specificity is described. The method involves the collection of radioactively labeled cells by aggregates of the same (isotypic aggregates) or different (heterotypic aggregates) types of tissue and determination of the number of cells collected by liquid scintillation counting. The use of (32)P to label the tissues permitted a much more rapid estimation of cell collection than was obtained previously. With the use of chick embryo neural retina, liver, forebrain, pectoral muscle, and heart ventricle tissue, it was shown that isotypic was always greater than heterotypic collection. Labeled neural retina cell collection by neural retina aggregates was studied as a function of time, cell suspension density, aggregate diameter, temperature, and aggregate number. Neural retina aggregates were treated with certain enzymes in an attempt to determine whether specific changes on the surface of the aggregates would interfere with labeled neural retina cell collection. Of the various proteases and glycosidases tested, only beta-galactosidase rendered the surface more nonspecific.


Defective cardiac parasympathetic control in patients with heart disease.


The runaway reaction.

The runaway reaction.


Isolation and biochemical characterization of nuclei from chick embryo liver. A procedure is described for the isolation of enzymatically active nuclei from chick embryo liver. It consists of the homogenization of the pooled tissue in 0.32 M sucrose-3 mM MgCl(2) followed by a slow centrifugation. The resulting nuclear pellet is then purified further in a discontinuous density gradient composed of sucrose solutions containing Mg(2+) ions, the lower portion of the gradient being 2.2 M sucrose-1 mM MgCl(2). Based on DNA recovery, the nuclear fraction isolated by the procedure described contained an average of 62% of the nuclei in the original filtered homogenate. Light and electron microscope examinations showed that 90% of the isolated nuclei were derived from hepatocytes. They appeared intact with well preserved nucleoplasmic and nucleolar components, nuclear envelope, and pores. The isolated nuclei were quite pure, having a very low level of cytoplasmic contamination as indicated by cytoplasmic enzyme marker activities and electron microscope studies. The nuclear fraction consisted of 19.9% DNA, 6.2% RNA, 74% protein, the average RNA/DNA ratio being 0.32. Biosynthetic activities of the two nuclear enzymes NAD-pyrophosphorylase and DNA-dependent RNA polymerase were preserved. The specific activities of these enzymes were: NAD-pyrophosphorylase, 0.049 micromoles nicotinamide adenine dinucleotide (NAD) synthesized/min per mg protein; Mg(2+) activated RNA polymerase, 4.3 micromicromoles UMP-2-C(14) incorporated into RNA/microg DNA per 10 min; and Mn(2+)-(NH(4))(2)SO(4) activated RNA-polymerase, 136 micromicromoles UMP-2-C(14) incorporated into RNA/microg DNA per 45 min.


A controlled trial of disodium cromoglycate in seasonal allergic rhinitis.


The regional distribution of glutamine synthetase (transferase) in brain of normal and chlorpromazine-treated rats.


Bicuculline, an antagonist of GABA and synaptic inhibition in the spinal cord of the cat.


Adrenergic receptors, bronchodilators and asthma.


Relation of a trainability index to T-group outcomes.


Cobalt experimental epilepsy in gerbils.


The spermatozoon of the jellyfish Nausithoë.


Prevention of indomethacin-induced intestinal ulcers by various catatoxic steroids.


Acetylcholine-induced stimulation of catecholamine recovery in denervated rat adrenals after reserpine-induced depletion.


A time sharing fluorometer for the readout of intracellular oxidation-reduction states of NADH and flavoprotein.


In vitro antimicrobial activity of 6(D(-) -amino-p-hydroxyphenylacetamido) penicillanic acid, a new semisynthetic penicillin.


Susceptibility of Diplococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis to 23 antibiotics.


Role of hepatic metabolism in the convulsant action of m-fluorotyrosine.


Gamma-glutamyl and D- or L-peptide linkages in mycobacillin, a cyclic peptide antibiotic. Mycobacillin lacks amino groups but contains two free alpha-carboxyl groups, indicating the presence of two side-chain peptide linkages. The five aspartic acid residues of mycobacillin are all in alpha-peptide linkage whereas the two glutamic acid residues are in gamma-linkage. Mycobacillin does not react with hydroxylamine to give hydroxamate, indicating the absence of anhydride, lactone and ester linkages. This is also confirmed by i.r. spectroscopy and titration of the molecule. Of the 15 peptides obtained from partial hydrolysates of mycobacillin, 12 contain aspartic acid. Results obtained by treatment of hydrolysates of aspartic acid-containing peptides with d-amino acid oxidase and l-glutamate decarboxylase (containing l-aspartate decarboxylase activity) indicate that residue 5 is l-aspartic acid and residues 2, 8, 11 and 13 are d-aspartic acid. The d- or l-peptide sequence and nature of peptide linkages in mycobacillin are proposed on the basis of these findings and the amino acid sequence reported earlier.


Allosteric interaction of D-glutamate with the glutamate dehydrogenases of Neurospora crassa.


Changes in metabolic intermediates during activation of Neurospora ascospores.


Photoperiodic control of the maintenance and termination of larval diapause in Wyeomyia smithii (Coq.) (Diptera: Culicidae) with notes on oogenesis in the adult female.


Effect of nicotamide on the regulation of carbohydrate metabolism in rat liver.


Studies of antihistaminics: dehydration of some substituted 4-amino-butan-2-ols.


Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.


Some developmental antecedents of psychopathology.


Interpersonal changes among psychiatric patients in human relations training.


In vitro induction of a heterograft reaction: immunological parameters of the sensitization of rat lymphocytes against mouse cells in vitro.


Collagen disease debuting as acute hepatitis.


Functional maturation of lymphocytes within embryonic mouse thymus.


Graft-versus-host assay to discriminate between mouse cells differing at weak histocompatibility loci.


Tubular reabsorption of sodium ion: influence of factors other than aldosterone and glomerular filtration rate. 2.


Clinical and pharmacological investigation of a new psychotropic drug sulpiride (Dogmatil).


The influence of regitine on the frequency of plethysmographic alpha waves.


Neuro- and psychotropic drugs in prescriptions of physicians in the district Prague 6.


Prescriptions of psychiatrists working in Prague policlinics.


Enzymatic properties of microsomal membranes from the protozoan Acanthamoeba castellanii.


Cerebral apoplexy (stroke): pathogenesis, pathophysiology and therapy as illustrated by regional blood flow measurements in the brain.


Alpha adrenergic stimulation of glucose uptake in human erythrocyte, lymphocyte, and lymphoblast.


Enhancement of morphine analgesia by a new adrenergic beta receptor antagonist, 1-isopropylamino-3(4-indanoxy)-2-propanol HC1 (USVC-6524).


Thyroxine induced metamorphosis in Aurelia.


Conformational studies of amphetamine and medicinally important derivatives by nuclear magnetic resonance spectroscopy.


A new series of semisynthetic rifamycins. N derivatives of 4-amino-4-deoxyrifamycin SV.


Verbal and spatial intelligence in short and long term alcoholics.


The influence of some cardioactive drugs on the energy-dependent uptake of calcium, potassium and adenine nucleotides by mitchondria.


The leopard (multiple lentigines) syndrome revisited.


The effect of alpha-adrenergic blockade on the cardiopulmonary response to ketamine.


Combined adrenergic receptor blockade in experimental posthaemorrhagic shock.


Hemodynamic responses to doxapram in normovolemic and hypovolemic dogs.


Effect of angiotensin on plasma-free fatty acids in dogs.


Unidirectional fluxes in isolated splenic nerve vesicles measured by a millipore filter technique: effects of noradrenaline and competitive reversal of reserpine inhibition.


Pornography: an experimental study of effects.


Effect of pyrazole in vivo on aldehyde metabolism in rat liver and brain.


Effect of protein-free diet on UDP-glucuronyltransferase and sulphotransferase activities in rat liver.


Influence of INPEA, pindolol and propranolol on the chronotropic and metabolic responses to  -adrenergic stimulation in intact rats.


Effects of lithium on brain adenyl cyclase activity.


Microsomal epoxidase activity in preparations of whole southern armyworm larvae.


Further studies on the submicrosomal distribution of drug-metabolizing components in liver. Localization in fractions of smooth microsomes.


Structured bioluminescence. Two emitters during both the in vitro and the in vivo bioluminescence of the sea pansy, Renilla.


Oxidized triphosphopyridine nucleotide specific isocitrate dehydrogenase from Azotobacter vinelandii. Modification of a reactive sulfhydryl group with cyanide.


Spectral properties, respiratory activity, and enzyme systems of bovine corpus luteum mitochondria.


A developmental concept of equine cryptorchism.


Role of reversed electron transport in bovine corpus luteum mitochondrial steroid synthesis.


Effects of gonadotropins and testosterone on the testes of prepubertal mice.


Glucose-6-phosphate dehydrogenase Boston. A new variant associated with congenital nonspherocytic hemolytic disease.


Pseudomonas osteomyelitis: puncture wounds of the feet.


Inhibition by homocysteine of serine dehydratase and other pyridoxal 5'-phosphate enzymes of the rat through cofactor blockage.


Pyridine nucleotide transhydrogenase from Azotobacter vinelandii cells: stereospecificity of hydrogen transfer.


Electrophoretic separation and characterization of the multiple forms of methemoglobin reductase.


Membrane structure and function. II. Alterations in the photo-induced absorption changes after treatment of isolated chloroplasts with large pulses of the ruby laser.


NADP-specific malate dehydrogenase of green spinach leaf tissue.


The glucose 6-phosphate metabolic crossroads in brain. Studies at the enzyme level.


Nicotinamide nucleotide synthesis in regenerating rat liver. 1. The concentrations and total content of the nicotinamide nucleotides were measured in the livers of rats at various times after partial hepatectomy and laparotomy (sham hepatectomy) and correlated with other events in the regeneration process. 2. The NAD content and concentration in rat liver were relatively unaffected by laparotomy, but fell to a minimum, 25 and 33% below control values respectively, 24h after partial hepatectomy. NADP content and concentration were affected similarly by both laparotomy and partial hepatectomy, falling rapidly and remaining depressed for up to 48h. 3. The effect of injecting various doses of nicotinamide on the liver DNA and NAD 18h after partial hepatectomy was studied and revealed an inverse correlation between NAD content and DNA content. 4. Injections of nicotinamide at various times after partial hepatectomy revealed that the ability to synthesize NAD from nicotinamide was impaired during the first 12h, rose to a peak at 26h and fell again by 48h after partial hepatectomy. 5. The total liver activity of NAD pyrophosphorylase (EC 2.7.7.1) remained at or slightly above the initial value for 12h after partial hepatectomy and then rose continuously until 48h after operation. The activity of NMN pyrophosphorylase (EC 2.4.2.12) showed a similar pattern of change after partial hepatectomy, but was at no time greater than 5% of the activity of NAD pyrophosphorylase. 6. The results are discussed with reference to the control of NAD synthesis in rapidly dividing tissue. It is suggested that the availability of cofactors and substrates for NAD synthesis is more important as a controlling factor than the maximum enzyme activities. It is concluded that the low concentrations of nicotinamide nucleotides in rapidly dividing tissues are the result of competition between NAD synthesis and nucleic acid synthesis for common precursor and cofactors.


The decarboxylation of retinoic acid by horseradish peroxidase and an acetone-butanol-ether-dried liver powder.


Immunological paralysis of mice with pneumococcal polysaccharide antigens.


A new coenzyme of methyl transfer, coenzyme M.


6-Methyltetrahydropterin. Isolation and identification as the highly active hydroxylase cofactor from tetrahydrofolate.


Adrenergic regulation of basal serum glucose, NEFA and insulin levels.


Some biochemical changes preceding regression of 7,12-dimethylbenz(a)anthracene-induced mammary tumors following oophorectomy.


Rapid identification of an arbovirus by observation on its morphogenesis in the electron microscope.


Inhibition of glyceraldehyde-3-phosphate dehydrogenase by follicle-stimulating hormone preparations in vitro.


Synthesis of new 5,8-quinolinequinones as inhibitors of coenzymes Q and as antimalarials.


Synthesis of some 5-substituted 10,11-dihydrodibenz[b,f]azepines.


Synthesis and pharmacological activity of dialkylaminoalkyl esters of benzilic acids containing fluorine or trifluoromethyl groups.


Effects of glucagon, prostaglandin E 1  and dibutyryl cyclic 3',5'-AMP upon the transmembrane action potential of guinea pig ventricular fiber and myocardial contractile force.


Task performance and task delegation in general practice. Implications for training of physician's assistants.


CNS activities of lactam derivatives.

CNS activities of lactam derivatives.


Treatment of angina pectoris with beta-receptor blocking agents. Effects of long-term treatment and treatment with sustained release tablets.


Inhibition of oxytocin-induced uterine activity in midpregnancy by combined adrenergic  -blockade and  -stimulation.


Lung puncture in the etiological diagnosis of pneumonia. A study of 543 infants and children.


Juvenile parkinsonism.

Juvenile parkinsonism.


A new health professional-the orthopedic assistant.


Premorbid adjustment and affective expression in schizophrenia.


Psychological factors in smoking, drinking, and drug experimentation.


Susceptibility to faking of the Psychological Screening Inventory.


An MMPI measure of maladjustment in a sample of "normal" homosexual men.


A comparison of studies of the Mini-Mult.


Distractibility as a function of MMPI HY and PT scores.


Self-disclosure and personality adjustment.


Normalization of verbal productivity and improvement in depressive status in schizophrenics.


Antihypertensive and monoamine oxidase inhibitory activity of some derivatives of 3-formyl-4-oxo-4H-pyrido (1,2-a) pyrimidine.


antihypertensive and monoamine oxidase inhibitory activity of some azacycloalkyl-substituted benzaldehyde hydrazone derivatives.


5-homopyridoxals, 5-thiopyridoxal, and related compounds. Synthesis, tautomerism, and biological properties.


Implication of bioamines in the x-ray-induced temperature response of cats and rabbits.


A comparative study on aflatoxin B 1  metabolism in mice and rats. In vivo metabolic studies on rats and mice revealed a marked difference in the fluorescent compounds produced after ingestion of aflatoxin B(1). The mouse converted aflatoxin B(1) to three unknown fluorescent compounds, designated x(1), x(2) and x(3) and the known aflatoxin M(1), while the rat was only capable of producing aflatoxin M(1). The results suggested that metabolites x(1), x(2), x(3) and aflatoxin M(1) were not part of a major metabolic pathway, but produced independently. These unknown yellowish-green fluorescent compounds did not seem to be conjugated with sulphate or glucuronic acid.In vitro incubations of various mouse liver cell fractions with aflatoxin B(1) showed that metabolites x(1), x(2), x(3) and aflatoxin M(1), could only be produced by the microsomal fraction and that NADPH was needed as a co-factor. The differences in aflatoxin metabolism by mice and rats are discussed in relation to the apparent resistance of the mouse to the carcinogenic effects of this toxin.


Sympathetic effects on fibrillary activity of denervated striated muscles.


Protection against induction of supersensitivity to catecholamines by cocaine. 1. Adrenaline, noradrenaline, isoprenaline, tyramine, phentolamine, pronethalol, histamine and acetylcholine were each tested for their ability to prevent cocaine from causing supersensitivity to catecholamines in cat spleen strips in vitro. A high concentration of one of these drugs was added to the bath 5 min before cocaine hydrochloride (10 mug/ml). The effect on subsequent responses to catecholamines was compared with the effect of cocaine in control strips in the absence of an interfering drug.2. Phentolamine completely abolished the potentiating effect of cocaine. Large doses of adrenaline or noradrenaline reduced, but did not completely prevent, potentiation. Tyramine, isoprenaline, pronethalol, histamine and acetylcholine did not prevent potentiation.3. The ability of these drugs to interfere with potentiation does not correlate well with their ability to interfere with uptake of noradrenaline. Interference with uptake by cocaine is therefore unlikely to account fully for potentiation.


Interaction between phosphodiesterase inhibitors and catecholamines on contractions of the cat soleus muscle.


Some observations on the estimation of 3-methoxytyramine in brain tissue. 1. A new method for the estimation of 4-hydroxy-3-methoxyphenylethylamine (3-methoxytyramine) in brain tissue is described. This is based on the formation of a fluorescent derivative by oxidation with potassium ferricyanide in ammonium hydroxide solution.2. The effects of some drugs on the concentration of 3-methoxytyramine in the brain are reported.3. The significance of changes in the striatal concentration of 3-methoxytyramine is discussed.


The pharmacology of inhibition of mitral cells in the olfactory bulb.


Enzymes of carbohydrate and amino acid metabolism in liver, brain and kidney of Macaca mulatta.


A review of Illinois' Clinical Laboratories and Blood Banks.


Utilization of nonaccepted premedical students.


Metabolic alterations in the human erythrocyte produced by increases in glucose concentration. The role of the polyol pathway. Human erythrocytes incubated in medium containing 50 mM glucose have increased intracellular sorbitol and fructose concentrations as compared with samples incubated with 5 mM glucose. Increased medium glucose concentration did not significantly alter total glucose consumption or lactate production. However, the intracellular lactate:pyruvate ratio rose, the concentrations of fructose diphosphate, and triose phosphates increased, and the 2,3-diphosphoglycerate concentration fell. [(14)C]O(2) production from glucose-1-(14)C also increased with increased medium glucose concentration. These changes are believed to reflect changes in the redox states of the diphosphopyridine nucleotide/reduced form of diphosphopyridine nucleotide (NAD/NADH) and nicotinamide-adenine dinucleotide phosphate/reduced form of nicotinamide-adenine dinucleotide phosphate (NADP/NADPH) couples resulting from increased activity of the polyol pathway. Addition of pyruvate to the incubation media prevented these changes. These studies illustrate that an increase in the red cell's normal substrate, glucose, can produce changes in red cell metabolism.


The chemotherapy of hypertension.

The chemotherapy of hypertension.


Actions of levodopa and dopamine in the central nervous system.


Inducible heme oxygenase in the kidney: a model for the homeostatic control of hemoglobin catabolism. We have recently identified and characterized NADPH-dependent microsomal heme oxygenase as the major enzymatic mechanism for the conversion of hemoglobin-heme to bilirubin-IXalpha in vivo. Enzyme activity is highest in tissues normally involved in red cell breakdown, that is, spleen, liver, and bone marrow, but it usually is negligible in the kidney. However, renal heme oxygenase activity may be transiently increased 30- to 100-fold following hemoglobinemia that exceeded the plasma haptoglobin-binding capacity and consequently resulted in hemoglobinuria. Maximal stimulation of enzyme activity in rats is reached 6-16 hr following a single intravenous injection of 30 mg of hemoglobin per 100 g body weight; activity returns to basal levels after about 48 hr. At peak level, total enzyme activity in the kidneys exceeds that of the spleen or liver. Cyclohexamide, puromycin, or actinomycin D, given just before, or within a few hours after, a single intravenous injection of hemoglobin minimizes or prevents the rise in renal enzyme activity; this suggests that the increase in enzyme activity is dependent on continued synthesis of ribonucleic acid and protein. The apparent biological half-life of renal heme oxygenase is about 6 hr. These observations indicate that functional adaptation of renal heme oxygenase activity reflects enzyme induction either directly or indirectly by the substrate, hemoglobin. Filtered rather than plasma hemoglobin appears to regulate renal heme oxygenase activity. Thus, stabilization of plasma hemoglobin in its tetrameric form with bis (N-maleimidomethyl) ether, which diminishes its glomerular filtration and retards it plasma clearance, results in reduced enzyme stimulation in the kidney, but enhances its activity in the liver. These findings suggest that the enzyme is localized in the tubular epithelial cells rather than in the glomeruli and is activated by luminal hemoglobin. Direct support for this concept was obtained by the demonstration of heme oxygenase activity in renal tubules isolated from rabbits that had been injected with hemoglobin.


The physician's associate in occupational medicine.


National Commission issues statement on physician assistants. Assistance for the physician: nurse clinician or physician assistant.


Utilization of corpsmen in the occupational health setting.


The nurse's role in meeting the challenges of medical care.


Contributions of the rat to the immunobiology of tissue transplantation.


Maternal-fetal interactions and histocompatibility antigen polymorphisms.


Transplantation in coelenterates.

Transplantation in coelenterates.


Transplantation in Cnidaria.

Transplantation in Cnidaria.


Specificity of allograft reaction in Eisenia foetida.


Tissue transplantation immunity and immunologic memory in Urodela and Apoda.


An epidemiological study of child health and nutrition in a northern Swedish county. VI. Relationship between general and oral health, food habits, and socioeconomic conditions.


Interaction of pyridoxal-5'-phosphate and acetohydroxy acid isomeroreductase.


Formation of meta-tyrosine form L-phenylalanine by beef adrenal medulla. A new biosynthetic route to catecholamines.


Kinetic properties of brain tyrosine hydroxylase and its partial purification by affinity chromatography.


Studies on energy-linked reactions. Energy-linked transhydrogenase reaction in Escherichia coli. 1. Small particles from Escherichia coli catalyse an ATP-dependent reduction of NADP(+) by NADH. 2. The reaction was stimulated by Mg(2+) and only ATP and ITP could serve as energy donors. 3. Studies of the stoicheiometry of the reaction indicate that 1-2mol of ATP are utilized/mol of NADH produced. 4. The reaction is inhibited by uncoupling agents such as 2,4-dinitrophenol (200mum) and tetrachlorotrifluorobenzimidazole (2mum) but oligomycin does not inhibit. 5. The reaction is inhibited by relatively high concentrations (200mum) of piericidin A.


The toxic proteins of an Australian jellyfish Chironex fleckeri.


The formation and reduction of the 14,15-double bond in cholesterol biosynthesis. It was shown that 100mug quantities of 4,4'-dimethyl[2-(3)H(2)]cholesta-8,14-dien-3beta-ol (IIIa), tritiated cholesta-8,14-dien-3beta-ol, 4,4'-dimethyl[2-(3)H(2)]cholesta-7,14-dien-3beta-ol, dihydro[2-(3)H(2)]lanosterol and [24-(3)H]lanosterol were converted by a 10000g supernatant of rat liver homogenate into cholesterol in 17%, 54%, 6%, 9.5% and 24% yields respectively. From an incubation of dihydro[3alpha-(3)H]lanosterol with a rat liver homogenate in the presence of a trap up to 38% of the radioactivity was found to be associated with a fraction that was unambiguously shown to be 4,4'-dimethylcholesta-8,14-dien-3beta-ol. Another related compound, 4,4'-dimethylcholesta-7,14-dien-3beta-ol was also shown to be equally effective in its ability to trap compound (IIIa) from an incubation of dihydro[3alpha-(3)H]lanosterol. The mechanism of the further conversion of the compound (IIIa) into cholesterol occurred by the reduction of the 14,15-double bond and involved the addition of a hydrogen atom from the medium to C-15 and another from the 4-position of NADPH to C-14. Two possible mechanisms for the removal of the 14alpha-methyl group in sterol biosynthesis are discussed.


Oxidation of cis- and trans-acenaphthene-1,2-diol by soluble preparations from rat liver.


Fatty acid synthetase from pig liver. 1. Isolation of the enzyme complex and characterization of the component with oxidoreductase activity for alicyclic ketones.


The purification and properties of the NAD-linked aldehyde dehydrogenase from pig brain.


A comparative study of the effect of graft-versus-host reaction and semi-allogeneic parabiosis on the survival of H-2 incompatible skin grafts in mice.


Guanosine diphosphate-4-keto-D-rhamnose reductase. A non-stereoselective enzyme.


Purification of reduced pyridine nucleotide dehydrogenase from human erythrocytes and methemoglobin reduction by the enzyme.


Administrative variables affecting the utility of an MMPI atlas.


Validity of the MMPI scales for measuring twenty psychiatric dimensions.


The impact of fluoridation on patterns of dental treatment.


Fluorides in community programs: results after two years from a fluoride gel applied topically.


Selected dental findings of a rural county in Appalachia.


Psychological aspects of contraception.

Psychological aspects of contraception.


Patterns of differentiation between human local populations.


Consideration of the therapeutic roles of pharmacologic agents, collateral circulation and saphenous vein bypass in coronary artery disease.


The electrophysiologic effects of antiarrhythmic drugs.


Avoidance conditioning for homosexuality.


The purification and characterization of acetoacetyl-coenzyme A reductase from Azotobacter beijerinckii. A soluble acetoacetyl-CoA reductase (EC 1.1.1.36) was purified 54-fold from Azotobacter beijerinckii N.C.I.B. 9067 and the reaction product identified as d(-)-beta-hydroxybutyryl-CoA. The Michaelis constants for acetoacetyl-CoA, NADPH and NADH were determined and the reaction rate was found to be some fivefold greater with NADPH than with NADH. At neutral pH the equilibrium greatly favours the formation of the reduced product. Substrate specificity was in the order: acetoacetyl-CoA>acetoacetylpantetheine>acetoacetyl-(acyl-carrier protein). The enzyme possesses a functional thiol group, suffers inactivation by oxygen and is inhibited by thiol-blocking reagents. Inhibition by p-chloromercuribenzoate is reversed by excess of dithiothreitol, which also protects the enzyme from inactivation by oxygen.


The effect of tannic acid on membrane enzymes and on permeability.


Lobster heart: electrophysiology of single cells including effects of the regulator nerves.


Epididymal carbohydrate metabolism. I. Glucose-1-14-C and glucose-6-14-C metabolism by mouse, rat and rabbit tissues.


Micropolarographic determination of oxygen uptake of 7-day chick neural retina cells.


Water turnover in Coturnix quail with individual observations on a burrowing owl, Petz conure and vulturine fish eagle.


Urea-N retention by Sylvilagus floridanus.


The effect of tagging upon the fatty acid metabolism of juvenile pink salmon.


Cation transport and metabolism as a function of salinity in the excised gill of Carcinus maenas.


A stimulatory effect of glucagon on DNA synthesis in neonatal rat liver.


Changes in the timing of lung ventilation and late foetal development in quail.


Cation transport and energy metabolism in the high Na+, low K+ erythrocyte of the harbor seal, Phoca vitulina.


The renin-angiotensin system in Bufo arenarum and Bufo paracnemis.


Renin-angiotensin system and sodium homeostasis in Bufo arenarum.


Viscous properties of muskox blood.

Viscous properties of muskox blood.


Localization of biogenic amines in Schistosoma mansoni.


Melatonin localization in the eyes of larval Xenopus.


Sympathetic versus parasympathetic nervous regulation of airways in dogs.


Microfluorometry of oxidation-reduction state of the rat kidney in situ.


Effects of Chrysaora quinquecirrha (Sea nettle) toxin on sodium transport across frog skin.


Drugs for problem school children.

Drugs for problem school children.


The concept of bioprosthesis.

The concept of bioprosthesis.


Effect of carbon tetrachloride induced progressive liver damage on drug-metabolizing enzymes and cytochrome P-450 in rat liver.


The use of allied health professionals.

The use of allied health professionals.


Reaction of reduced flavins with tetranitromethane.


The relationship of soluble and mitochondrial isocitrate dehydrogenases in metabolic regulation.


Characteristics of lysosomes in the rat placental cells.


Macrophage metabolism: activation of NADPH oxidation by phagocytosis.


Homoserine dehydrogenases of Serratia marcescens S a -3.


Release of glucose repression of oxidative phosphorylation in Escherichia coli B by cyclic adenosine 3',5'-monophosphate.


Evidence for two nicotinamide binding sites on L-glutamate dehydrogenase.


A study of spin-probe solubility in mitochondrial membranes correlated with ATP-dependent conformational changes.


Activation of CO 2  fixation in isolated spinach chloroplasts.


Pigment systems and electron transport in chloroplasts. I. Quantum requirements for the two light reactions in spinach chloroplasts.


Observations on photosystem II mutants of Scenedesmus: pigments and proteinaceous components of the chloroplasts.


Plastocyanin participation in chloroplast photosystem I.


Concepts of fatigue and vigilance in relation to railway operation.


Assessment criteria for mental fatigue. A factor-analytic study of phase discrimination in mental fatigue.


Physiological parameters of mental load.


Fatigue assessment on key punch operators, typists and others.


Maintaining concentration (TAF) as a measure of mental stress.


Fatigue and stress in air traffic controllers.


Fatigue from the point of view of urinary metabolites.


Physician's assistants legislation.

Physician's assistants legislation.


Physician's assistants for ophthalmologists.


Rates of ethanol clearance and activities of the ethanol-oxidizing enzymes in chronic alcoholic patients.


Derivatives of 4-azahomoadamantane. Their synthesis and biological evaluation.


3-alkyltyrosines. Potential antihypertensive agents.


An electron microscopic study of the laminar pattern and mode of termination of afferent fibre pathways in the somatic sensory cortex of the cat.


Steps in the reactions of proteolytic enzymes with their substrates.


The three-dimensional structure of crystalline porcine pancreatic elastase.


A hydrogen-bond network at the active site of subtilisin BPN'.


II. Acid proteinases. Chemical studies of porcine pepsin.


On the mechanism of action of pepsin.

On the mechanism of action of pepsin.


The N-terminal and C-terminal amino acid sequence of calf rennin.


X-ray diffraction studies of rennin crystals.


3. Carboxypeptidase. Bovine carboxypeptidase A--activation, chemical structure and molecular heterogeneity.


The structure of carboxypeptidase A. 8. Atomic interpretation at 0.2 nm resolution, a new study of the complex of glycyl-L-tyrosine with CPA, and mechanistic deductions.


Chemical approaches to the mode of action of carboxypeptidase A.


IV. Cysteine proteinases. The structure of the papain molecule.


The structure and mechanism of action of papain.


Mapping the active site of papain with the aid of peptide substrates and inhibitors.


I. Serine proteinases. The structure of alpha-chymotrypsin.


Homologies in serine proteinases.

Homologies in serine proteinases.


Studies of the activity of chymotrypsin.


Neurovenous tissues in cephalopods.

Neurovenous tissues in cephalopods.


Are oocytes formed and used sequentially in the mammalian ovary?


Germ cells in natural and experimental chimeras in mammals.


How does the Y chromosome affect gonadal differentiation?


Hormonal factors in the sex differentiation of the mammalian foetus.


In vitro studies on differentiation of the reproductive tract.


The bovine freemartin: a new look at an old problem.


Experimental control of psychosexuality.


Unusual sex chromosome inheritance in mammals.


Hormonal differentiation of the developing central nervous system with respect to patterns of endocrine function.


Antiandrogens and reproductive development.


Hormonal and clinical aspects of hermaphroditism and the testicular feminizing syndrome in man.


The Xg blood groups and sex-chromosome aneuploidy.


Genetic basis for the determination of sex.


Genetic activity of sex chromosomes in somatic cells of mammals.


Genetic activity of sex chromosomes in germinal cells.


Natural sex reversal in vertebrates.

Natural sex reversal in vertebrates.


Germ-cell line and sexual differentiation in birds.


Migration of gonocytes into the mammalian gonad and their differentiation.


Studies on pathogenic and non-pathogenic small free-living amoebae and the bearing of nuclear division on the classification of the order amoebida.


Cardiovascular responses in rabbits of different ages to hypertensin and adrenaline.


Basic studies on Hirudo medicinalis for a space experiment. II. Behavior of Hirudo medicinalis in unnatural environments.


Multi-purpose monitoring of carbon dioxide in closed organism-environment systems. Suggestion for biosatellites.


Microbial contamination associated with the Apollo 6 spacecraft during final assembly and testing.


Biological evaluation of various spacecraft cabin atmospheres. I.


Biochemical and metabolic effects of a six-month exposure of small animals to a helium-oxygen atmosphere.


First permanent molars of six-year-old children after two and a half years of fluoridation in Western Australia.


Autoradiography of some suspected neurotransmitter substances: GABA glycine, glutamic acid, histamine, dopamine, and L-dopa.


Host response to challenge with SA7-induced autochthonous tumor cells.


Immunological studies on a hamster-specific sarcoma virus.


Immunosuppression by the radiation leukemia virus and its relation to lymphatic leukemia development.


Glycine uptake in rat central nervous system slices and homogenates: evidence for different uptake systems in spinal cord and cerebral cortex.


The desmosterol reductase activity of rat brain during development.


Catecholamine depletion and adrenergic neurone blockade: studies with debrisoquine.


Effects of some drugs on capillary permeability in the anaphylaxis of the mouse.


Incidence of congenital abnormalities in a beagle colony.


Galactose-metabolizing enzymes in the rat.


Effects of acute infection on cholesterogenesis in the rhesus monkey.


The effects of a new adrenergic antagonist on the mesenteric circulation.


Characterization of the endogenous mediator(s) of serum zinc and iron depression during infection and other stresses.


Effect of strong and weak histocompatibility antigens on the mixed lymphocyte culture reaction in rats.


On the presence of viruses of the American equine encephalomyelitis in Central Europe. Review.


The effect of testosterone on ascorbic acid synthesis in rooster comb.


Presence of adrenergic receptors in the uterus of two species of lizards, Liolaemus gravenhorti and Liolaemus tenuis tenuis.


Prognostic significance of the determination of oxidoreductase activity in bone marrow smears from children with acute leukaemia.


A plea for the physician's assistant.

A plea for the physician's assistant.


American Association of Medical Assistants, State of Iowa, Inc.


Hepatitis-associated (Australia) antigen: a review.


The daytime distribution of mosquitoes resting in vegetation.


Studies on the mosquitoes of North Arcot district, Madras State, India. 3. Host preferences for pigs, birds and some small mammals.


The effect of beta-adrenergic blocking agents on serum lipids.


Studies on the mosquitoes of North Arcot District, Madras State, India. 4. Host preferences as shown by precipitin tests.


New collection records of Psorophora ciliata (Fabricius), Psorophora ferox (Humboldt) and Anopheles earlei Vargas in Connecticut (Diptera: Culicidae).


Dynamic characteristic of the 'functional compartment' of dopamine in dopaminergic terminals of the rat striatum.


Chronic propranolol administration during pregnancy. A case report.


Abortion. Is it a therapeutic procedure in psychiatry?


Acute iron poisoning in children. The problem of appropriate therapy.


Medical education in the United States. 1970-1971. V. Educational activities in areas allied with medicine.


The synaptic organization of the caudate nucleus.


The site of termination of afferent fibres in the caudate nucleus.


The connexions of the striatum and globus pallidus: synthesis and speculation.


A program for the study of long-term adaptation to a weightless environment providing three-dimensional freedom of movement.


Space biomagnetics.

Space biomagnetics.


Space clinical medicine. A prospective look at medical problems from hazards of space operations.


Confinement and free-volume requirements.


Genetic interaction between two influenza A-WS virus mutants. II. Genetic interaction between NWS-D and WS-MK viruses in human diploid cells and hamster embryo fibroblasts.


Genetic interaction between two influenza A-WS virus mutants. I. Characterization of the mutants.


Interaction of plasma membranes with influenza virus. II. Release of viral nucleoprotein.


Sensitivity of A2-Hong Kong and pre-1968 A2 influenza virus strains to normal serum inhibitors.


Puromycin as an inhibitor of parainfluenza 3 virus multiplication.


Interference activity and sensitivity to interferon of original and "cold" strains of adenovirus types 1 and 2.


Interferon as viral inhibitor induced by heterologous DNA.


Studies with kemerovo virus in Singh's Aëdes cell lines.


Alfalfa mosaic virus in lucerne plants and its transmission by aphids in the course of the vegetation period.


Inhibitory action of N-2-hydroxyphenylthiourea derivatives on picornavirus multiplication in cell cultures.


Studies on trypsin-resistance, spermagglutination and spermadsorption of equine herpesviruses.


Efficiency of alfalfa mosaic virus transmission by aphids in relation to the level of virus in plants.


Powassan virus infection in the grey squirrel.


Neutralizing antibody formation in newborn colostrum-deprived germfree piglets infected per os with Teschen disease virus.


Interferon induced by polyinosinic-polycytidylic acid in interferon pretreated L cells in absence of DEAE-dextran.


Action of cinnarizine on the immunohaemolytic system.


Differentiation of receptors activated by catecholamines. 2.


Angiotensin and biosynthesis of norepinephrine.


Effect of L-dopa, adrenergic  -blockers and anticholinergic agents on the tremorine-tremor in mice.


Effect of morphine and nalorphine on the intestinal motility of the cat.


Direct evidence for the formation of an acyl phosphate by glutamine synthetase.


The passenger cell concept in transplantation immunology.


A fatty acid gamma-hydroxylation system solubilized from porcine kidney cortex microsomes.


Strain differences in the antibody plaque-forming cell responses of inbred mice to pneumococcal polysaccharide.


Ontogeny of cellular immunity: development in rat thymocytes of mixed lymphocyte reactivity to allogeneic and xenogeneic cells.


A histochemical study of retinal changes induced by treatment with colchicine.


Detection of bacteremia after the use of an oral irrigation device in subjects with periodontitis.


Genital abnormalities in male patients with cystic fibrosis.


Cyclic adenosine 3',5'-monophosphate during glucose repression in the rat liver. Intragastric administration of glucose inhibits the induction of serine dehydratase and tyrosine aminotransferase by glucagon in rat liver, but has no effect on the increase in hepatic adenosine 3',5'-monophosphate resulting from administration of glucagon. Thus, glucose repression in mammalian liver, unlike catabolite repression in microorganisms, appears to operate independently of the amounts of cyclic nucleotide in the cells.


The use of a combination of cytostatics with substances influencing neuro-humoral regulation (K6) in the treatment of lung cancer.


"Chin-sternum-heart syndrome": cardiac injury associated with parachuting mishaps.


A study of dental needs, DMF, def, and tooth eruption in migrant Negro children.


Monoamine oxidase: multiple forms and selective inhibitors.


Effects of propyl gallate and other antioxidants on rat liver microsomal nicotinamide-adenine dinucleotide phosphate-linked cytochrome c reductase.


Decayed, missing, and filled teeth among children, U.S.


Inflammation of arteries.

Inflammation of arteries.


Antigen-induced selective recruitment of circulating lymphocytes.


The heterogeneity of lymphocytes and their interaction.


Graft-versus-host reactivity of human lymphoid cells from established cell lines.


Susceptibility to audiogenic stimuli induced by hyperbaric oxygenation and various neuroactive agents.


Drug actions on the central nervous system as studied by the effects on the electroretinogram.


Delayed primary operation for open injuries of the extremities, especially the hand (two-stage treatment).


Lymphocyte transformation with bacterial antigens in intrinsic asthma.


Bidirectional tachycardia.

Bidirectional tachycardia.


Potassium ion release and enzyme secretion: adrenergic regulation by alpha- and beta-receptors. Epinephrine caused amylase secretion and K(+) release in rat parotid slices. Propranolol, which blocks beta-receptors, inhibited amylase secretion; phentolamine, which blocks alpha-receptors, inhibited K(+) release. Since enzyme secretion was associated with fusion of secretory granules to the cell membrane and K(+) release was associated with vacuole formation, it could be shown that both alpha- and beta-receptors are present in the same exocrine cell. The findings appear to exclude cyclic 3',5'-adenosine monophosphate as an intermediate in the alpha-receptor response.


Modification of graft-versus-host disease by neuraminidase treatment of donor cells. Decreased tolerogenicity of neuraminidase-treated cells.


Suppression of graft-versus-host-reactive lymphocytes by heterologous antithymus serum in vitro.


Application of marrow grafts in human disease.


Sterilisation of cattle by induced cryptorchidism.


The biosynthesis of proline in Escherichia coli: phosphate-dependent glutamate  -semialdehyde dehydrogenase (NADP), the second enzyme in the pathway.


Use of bundle of His potentials to assess changes in atrioventricular conduction produced by a series of beta-adrenergic blocking agents.


Directional signals for nursing's expanding role.


The reversible inhibition of catalase activity by nucleotides and its possible relationship to mouse liver catalase depression induced by biological substances.


Differential response to psychotropic drugs.


Mechanism of acute hemodynamic response to ureteral occlusion.


Tolerance induction and sensitization by preincubation of bone marrow with allogeneic erythrocytes.


The practice of clinical pharmacology.

The practice of clinical pharmacology.


Surgical treatment of multi-glandular syndromes.


Recent progress in the freeze-etching technique.


Freeze-etching studies of membrane structure.


Freeze-etching studies on muscle.

Freeze-etching studies on muscle.


Electron microscopic studies of macromolecules without appositional contrast.


Individual macromolecules: preparation and recent results with DNA.


Application of optical diffraction to helical structures in the bacteriophage tail.


Optical diffraction studies of myofibrillar structure.


Three-dimensional reconstruction of the stacked-disk aggregate of tobacco mosaic virus protein from electron micrographs.


Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs.


Current and future prospects in electron microscopy for observations in biomolecular structure.


Phase contrast and interference microscopy with the electron microscope.


Neurohypophyseal hormones and sodium transport.


The electrical response of isolated salivary glands during stimulation with 5-hydroxytryptamine and cyclic AMP.


Sodium-hexose interactions.

Sodium-hexose interactions.


Water-solute coupling and ion selectivity in epithelia.


Sodium-amino acid interactions in the intestinal epithelium.


Salt transport across isolated frog skin.


Anionic and cationic exchange mechanisms in the skin of anurans, with special reference to leptodactylidae in vivo.


Electrolyte transport in kidney tubule cells.


The energetic coupling of acid secretion in gastric mucosa.


Ion transport across rumen and omasum epithelium.


Initiation of salt and water transport in mammalian salivary glands by acetylcholine.


The stimulation of sodium transport by aldosterone.


A discussion on active transport of salts and water in living tissues. Introductory remarks.


Some "partial reactions" of the sodium pump.


Summary of the Third International Congress of the Transplantation Society: a personal approach.


Metabolic pathways of bile acid synthesis.


Regulation of hepatic enzyme synthesis by cyclic AMP.


Enzymatic regulation of bile acid synthesis.


Supercooling in reptiles and other vertebrates.


Ocular oxygen concentrations accompanying severe chronic ophthalmic pathology in the lake trout (Salvelinus namaycush).


Relationship of isoproterenal and beta-adrenergic blockade to serum potassium.


Osmoregulation in the trematode Haematoloechus medioplexus (Looss).


Effects of acclimation temperature on brown adipose tissue in the red squirrel (Tamiasciurus hudsonicus).


The renal reabsorption of arginine, lysine and ornithine in the young cockerel (Gallus domesticus).


Reduced evaporation in house mice(Mus musculus) acclimated to water restriction.


Adrenaline activity on extracellular fluid compartments of the lateral muscle of the rainbow trout (Salmo gairdnerii richardson): effect of adrenergic blocking agents.


Seasonal changes of metabolism in Arianta arbustorum (Gastropoda; Pulmonata): the cholinesterase of the blood.


Hormonal and dietary control of enzymes in the rat. IV. Dietary control of some liver enzymes unique to amino acid metabolism and gluconeogenesis and its dependence on pancreatic hormones.


Kinetic studies on the interaction of D-amino acid oxidase with benzoate and o-aminobenzoate.


Hydrophobic enzymes in hydrocarbon degradation.


Life support systems and the ecology.

Life support systems and the ecology.


The induction of murine neoplasia.

The induction of murine neoplasia.


Transplantation of small bowel in the rat: technical and immunological considerations.


Porphyrinsynthesis in isolated particles from tissue cultures of tobacco.


Interactions between ferredoxin and chlorophyll in a monolayer system.


Early functions of poliovirus. II. The RNA replication of hydroxylamine-inactivated polioviruses.


Early functions of poliovirus. 3. The effect of guanidine on early functions.


Validity of a linear Hammett plot for the stability of some carcinogenic 1-aryl-3,3-dimethyltriazenes in an aqueous system.


Growth rate measurements in gamma irradiated catfish.


Photosynthesis: the electron transport system of green plants.


Chemistry of the streptovaricins. 8. Structures of streptovaricins A, B, D, E, F, and G.


Chemistry of the streptovaricins. IX. X-ray crystallographic structure of a streptovaricin C derivative.


Affinity chromatography.

Affinity chromatography.


Recent advances in acute glomerulonephritis.


The child-health nurse (pediatric nurse practitioner) in private practice. A controlled trial.


Treatment of acute barbiturate intoxication.


Successful bone-marrow transplantation in a lymphopenic immunologic deficiency syndrome.


Humoral blockade on lethal pulmonary embolism in the awake dog.


The role of the general practitioner in the early management of acute myocardial infarction.


Renal allograft survival in bone marrow chimeras.


Effects of catecholamines on acid secretion by bullfrog isolated gastric mucosa.


A dental epidemiological survey of a northern Ireland population: dental caries and periodontal disease findings.


The effect of imipramine on in vitro dog bladder muscle contractility.


Treatment of severe spinal cord injuries by biochemical norepinephrine manipulation.


A new equation for microbial growth.

A new equation for microbial growth.


Early functions of poliovirus. I. The inhibition of cellular protein synthesis in HeLa cells after infection with active and inactivated poliovirus.


Incorporation of uridine in cleavage stage eggs of the sea urchin Paracentrotus lividus.


Rhythmic incorporation of P 32  and C 14 -uracil in early mitotic cycles of Limnaea (mollusc) eggs.


Differences in regulation between rat liver and kidney tyrosine aminotransferases.


Flash photolysis of rhodopsin. II. Measurements on rhodopsin digitonin solutions and fragments of rod outer segments.


Activation by bicarbonate orthophosphate, and sulfate of rat liver microsomal glucose dehydrogenase.


Di- -glutamylcystine as a substrate for glutathione reductase.


A review of isozymes in cancer.

A review of isozymes in cancer.


Induction of tyrosine aminotransferase and amino acid transport in Morris hepatomas and in adult and neonatal rat liver.


Glucose 6-phosphate dehydrogenase in the mammalian lens.


Population age 65+: special issue on geriatrics.


Psycho-social aspects of aging.

Psycho-social aspects of aging.


Management of orthopedic problems in the aged.


Arthritis in the aged.

Arthritis in the aged.


Advances in the management of Parkinson's disease.


Normal aging.

Normal aging.


Nursing home: medical director's role.

Nursing home: medical director's role.


Marjolin's ulcer.

Marjolin's ulcer.


Appendico-enteric fistula.

Appendico-enteric fistula.


The treatment of acute alcoholism in a small rural hospital.


Control of hypertension: an unmet challenge.


Buying a medical building: a sound investment?


Patient experiencing and successful outcome in individual psychotherapy of schizophrenics and psychoneurotics.


The child health associate.

The child health associate.


Use of the embryonating egg in studies on Marek's disease.


Identification of dangerous drugs by mass spectrometry.


Fever of unknown etiology.

Fever of unknown etiology.


Time-dependence of ALS effects on immunological and haematopoietic activities in mice.


Dental caries experience of 2,831 elementary children recorded and analyzed.


In vitro basilar and common carotid artery reactivity to alpha and beta stimulation.


The role of the pediatric allergist in the care of patients with eustachian tube dysfunction.


On further conjectures regarding acquiescence.


Identification of content and style: a two-dimensional interpretation of acquiescence.


Early or late operation in the treatment of Crohn's disease.


Skin reactions of natives in the Western Highlands of New Guinea to an antigen prepared from Dirofilaria Immitis.


Studies on adrenaline-induced leucocytosis in normal man. II. The effects of   and   adrenergic blocking agents.


Uptake by brain and distribution of radioactivity after intravenous administration of  14 C-labelled meclofenoxate in mice.


New inhalation anesthetics.

New inhalation anesthetics.


Effects of anesthesia on the peripheral circulation.


The effect of enzymes and other reagents on NADPH oxidation and oxygen uptake by microsomal preparations from the housefly, Musca domestica.


Oxygen uptake by microsomal preparations from the housefly, Musca domestica.


Relationship between energy substrate utilization and specific growth rate in Aspergillus nidulans. The maintenance coefficient of glucose-limited Aspergillus nidulans chemostat cultures at 30 C was 0.018 g per g (dry weight) per hr for glucose and 0.55 mmoles per g (dry weight) per hr for oxygen. These values can only be approximate because melanin was produced by the mold at low growth rates and because it is unlikely that this polymer contributed to the maintenance energy requirement although it contributed to the dry weight. Biomass (defined here as dry weight minus melanin) was used to calculate a more meaningful maintenance coefficient for glucose (0.029 g of glucose per g of biomass per hr). At the highest growth rates examined, a nonlinear relationship between growth rate and glucose utilization rate was obtained, suggesting a qualitative change in the metabolic activities of the mold at high growth rates. The oxidative capacity of the mold was highest at the highest growth rates. This observation indicates that the increased substrate utilization rate observed at the higher growth rates is a reflection of enhanced enzyme synthesis. This hypothesis was verified by assaying the specific activities of several enzymes at different growth rates. However, in contrast to all the other enzymes assayed, the activities of reduced nicotinamide adenine dinucleotide phosphate: (acceptor) oxido-reductases were highest at the lowest growth rates.


Heterotrophic metabolism of the chemolithotroph Thiobacillus ferrooxidans. Glucose-6-phosphate dehydrogenase and the enzymes of the Entner-Doudoroff pathway, 6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase (assayed together), are induced during heterotrophic growth of Thiobacillus ferrooxidans on an iron-glucose-supplemented medium or on glucose alone. By contrast, autotrophic cells (iron-grown) contain low levels of these enzymes. Fructose 1, 6-diphosphate aldolase, an enzyme of the Embden-Meyerhof pathway, is present at low levels irrespective of the growth medium, suggesting that this enzyme is not involved in energy-yielding reactions but merely provides intermediates for biosynthesis. The Entner-Doudoroff and pentose-phosphate pathways are the principle means through which glucose is dissimilated and is presumed to be concerned with energy production. Isotopic studies showed that a high rate of CO(2) formation from specifically labeled glucose came from carbon atoms 1 and 4. An unexpectedly high rate of evolution of CO(2) also came from carbon 6, suggesting that the triose phosphate formed during glucose breakdown and specifically as a result of 2-keto-3-deoxy-6-phosphogluconate aldolase activity, was metabolized via some unorthodox metabolic route. Cells grown in the iron-supplemented and glucose-salts media have a complete tricarboxylic acid cycle, whereas autotrophically grown T. ferrooxidans lacked both alpha-ketoglutarate dehydrogenase and reduced nicotinamide adenine dinucleotide oxidase. Two isocitrate dehydrogenases [nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP) specific] were present. NAD-linked enzyme was constitutive, whereas the NADP-linked enzyme was induced upon adaptation of autotrophic cells to heterotrophic growth.


Glucose-6-phosphate dehydrogenase from the chemolithotroph Thiobacillus ferrooxidans. Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 110,000 as determined by gel electrophoresis. Evidence is presented that T. ferrooxidans glucose-6-phosphate dehydrogenase is identical when isolated from cells grown mixotrophically (iron-glucose grown) or cells grown heterotrophically (glucose-grown cells). The enzyme is activated by Mg(2+), and to a lesser extent by low concentrations of Mn(2+). Reduced NAD inhibits the enzyme from T. ferrooxidans. No deviation from normal Michaelis-Menten kinetics was observed in velocity versus substrate concentration experiments. Adenosine triphosphate exerted a profound inhibition of the enzyme; the effect was 10 times more pronounced in the presence of NAD as compared to NADP. The physiological significance of this inhibition is discussed.


Repression of aromatic amino acid biosynthesis in Escherichia coli K-12. Mutants of Escherichia coli K-12 were isolated in which the synthesis of the following, normally repressible enzymes of aromatic biosynthesis was constitutive: 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetases (phe and tyr), chorismate mutase T-prephenate dehydrogenase, and transaminase A. In the wild type, DAHP synthetase (phe) was multivalently repressed by phenylalanine plus tryptophan, whereas DAHP synthetase (tyr), chorismate mutase T-prephenate dehydrogenase, and transaminase A were repressed by tyrosine. DAHP synthetase (tyr) and chorismate mutase T-prephenate dehydrogenase were also repressed by phenylalanine in high concentration (10(-3)m). Besides the constitutive synthesis of DAHP synthetase (phe), the mutants had the same phenotype as strains mutated in the tyrosine regulatory gene tyrR. The mutations causing this phenotype were cotransducible with trpA, trpE, cysB, and pyrF and mapped in the same region as tyrR at approximately 26 min on the chromosome. It is concluded that these mutations may be alleles of the tyrR gene and that synthesis of the enzymes listed above is controlled by this gene. Chorismate mutase P and prephenate dehydratase activities which are carried on a single protein were repressed by phenylalanine alone and were not controlled by tyrR. Formation of this protein is presumed to be controlled by a separate, unknown regulator gene. The heat-stable phenylalanine transaminase and two enzymes of the common aromatic pathway, 5-dehydroquinate synthetase and 5-dehydroquinase, were not repressible under the conditions studied and were not affected by tyrR. DAHP synthetase (trp) and tryptophan synthetase were repressed by tryptophan and have previously been shown to be under the control of the trpR regulatory gene. These enzymes also were unaffected by tyrR.


Phenylalanine and tyrosine biosynthesis in Escherichia coli K-12: mutants derepressed for 3-deoxy-D-arabinoheptulosonic acid 7-phosphate synthetase (phe), 3-deoxy-D-arabinoheptulosonic acid 7-phosphate synthetase (tyr), chorismate mutase T-prephenate dehydrogenase, and transaminase A. Mutant strains of Escherichia coli have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (phe) is derepressed, in addition to those enzymes of tyrosine biosynthesis previously shown to be controlled by the gene tyrR. The major enzyme of the terminal pathway of phenylalanine biosynthesis chorismate mutase-prephenate dehydratase is not derepressed in these strains. Genetic analysis of the mutants shows that the mutation or mutations causing derepression map close to previously reported tyrR mutations. A study of one of the mutations has shown it to be recessive to the wild-type allele in a diploid strain. It is proposed that the tyrR gene product is involved in the regulation of the synthesis of DAHP synthetase (phe) as well as the synthesis of DAHP synthetase (tyr), chorismate mutase-prephenate dehydrogenase, and transaminase A.


Metabolism of benzoic acid by bacteria: 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid is an intermediate in the formation of catechol. 3,5-Cyclohexadiene-1,2-diol-1-carboxylic acid (1,2-dihydro-1,2-dihydroxy-benzoic acid) is converted enzymatically to catechol in cell extracts from Acinetobacter, Alcaligenes, Azotobacter, and three Pseudomonas species. This enzymatic activity is present only in cultures which have been grown in the presence of benzoic acid, and which convert benzoic acid to catechol rather than to protocatechuic acid. The reaction is assayed by the concomitant formation of reduced nicotinamide adenine dinucleotide from nicotinamide adenine dinucleotide. The conversion of [(14)C]benzoic acid to [(14)C]dihydrodihydroxybenzoic acid is demonstrated in cell extracts. A scheme for the conversion of benzoic acid to catechol in bacteria is presented, involving the formation of dihydrodihydroxybenzoic acid from benzoic acid by a dioxygenase which is unstable in cell extracts, followed by the dehydrogenation and decarboxylation of dihydrodihydroxybenzoic acid to catechol by a previously undescribed enzyme. Experiments with anthranilic acid and phthalic acid suggest that dihydrodihydroxybenzoic acid is a metabolite unique to benzoic acid metabolism. Two new methods for assaying benzoic acid dioxygenase are suggested.


A comparative study on the biosynthesis of palmitic and orsellinic acids in Penicillium baarnense.


Neuromuscular transmission of trophic effects.


Diagnostic tests in myasthenia gravis: an overview.


An N-methyl glutamate dehydrogenase from Pseudomonas M.A.


Dihydrofolate reductase from diplococcus pneumoniae. Purification, amino acid composition, and N-terminal amino acid analysis.


Enzymatic basis of metabolic stimulation in leucocytes during phagocytosis: the role of activated NADPH oxidase.


Induction of tyrosine hydroxylase by dibutyryl adenosine 3',5'-monophosphate in guinea pig adrenal slices.


The distinct nature and function of NADPH-cytochrome c reductase and the NADPH-dependent mixed-function amine oxidase of porcine liver microsomes.


Lack of correlation between cyanide-binding spectrum and fatty acid desaturase activity in liver microsomes.


Oxidation-reduction mechanisms of cytochrome P-450.


Modulation of threonine control of aspartokinase activity.


Simple and combined adrenergic receptor blockade in canine endotoxinemia.


Introduction to discussion of lens and red cell sulfhydryl membrane groups.


Metabolism of glutathione in the lens.

Metabolism of glutathione in the lens.


Rural medical care. Physician's assistant linked to an urban medical center.


Polyarteritis nodosa in a child.

Polyarteritis nodosa in a child.


Demonstration of cytochrome P-450 in human foetal liver microsomes in early pregnancy.


Nephrographic patterns in renal diagnosis.


The role of multiple molecular forms of glutamine synthetase in the regulation of glutamine metabolism in Escherichia coli.


Macroradiography and small vessels of the kidney.


Rapid kinetic studies of the light emitting protein aequorin.


The function of the interdental cells of the limbus spiralis. A cytoenzymological study.


A 10-year study of bacteremia.

A 10-year study of bacteremia.


Stress-related effects of various inhibitors of catecholamine synthesis in the mouse.


The effect of pretreatment with phenobarbital on the teratogenic action of chorcyclizine in mice.


Isolation and properties of a 'malic' enzyme from cauliflower bud mitochondria. 1. A ;malic' enzyme [l-malate-NAD oxidoreductase (decarboxylating), EC 1.1.1.39] has been isolated from cauliflower bud mitochondria and partially purified. 2. The enzyme is specific for l-malate and has an absolute requirement for either Mn(2+), Co(2+) or Mg(2+). 3. The enzyme shows activity with both NAD(+) and NADP(+), but NAD(+) is the preferred cofactor. 4. No appreciable oxaloacetate decarboxylase activity is present in the enzyme preparations even at low pH values. 5. The enzyme is inhibited by NADH and by oxaloacetate and stimulated by SO(4) (2-) and by low concentrations of CoA. 6. The regulatory properties of the enzyme support the proposed role of the enzyme in the utilization of tricarboxylic acid-cycle acids for energy production when glycolysis is suppressed.


Intravenous practolol.

Intravenous practolol.


Uterus presenting in inguinal hernia of a male pseudo-hermaphrodite.


Studies on the interaction between growth hormone and dihydroergotamine in adipose cells. I. The lipolytic effect of dihydroergotamine.


Effect of riboflavin depletion and repletion on the erythrocyte glutathione reductase in the rat.


The effect of bradykinin upon human and rat myometrium contractility after blockade of adrenergic receptors in vitro.


The effect of fibrinogen degradation products upon the  -and  -receptors of the adrenergic system in the rat uterus.


MMPI: comparison of the oral form presented by a live examiner and the booklet form.


An MMPI index of disturbed marital interaction.


Effect of post-trial injection of beta adrenergic blocking agents on a conditioned reflex in rats.


Isolation and partial purification of a toxin from Millepora alcicornis.


Isolation and purification of a toxin from Millepora dichotoma.


Some pharmacological properties of the venom of the scorpionfish Scorpaena guttata. I.


Effect of proteolytic enzymes on the venom of the sea wasp Chironex fleckeri.


Escape of mesenteric vessels from adrenergic and nonadrenergic vasoconstriction.


Effects of altered autonomic control on left ventricular function in conscious dogs.


The patient's meanings.

The patient's meanings.


Penicillamine and analogous chelating agents.


Temperature and enzyme activity in poikilotherms. Isocitrate dehydrogenases in rainbow-trout liver. 1. The kinetics of the thermally induced enzyme variants of the supernatant NADP-isocitrate dehydrogenase from rainbow-trout liver are investigated. 2. Fish acclimatized to 2 degrees C (cold-adapted enzyme) and 17 degrees C (warm-adapted enzyme) show different relative distributions of the three NADP-isocitrate dehydrogenase isoenzymes; this has been demonstrated with electrophoresis and electrofocusing techniques. 3. Plots of K(m) versus temperature for the cold-adapted and warm-adapted enzyme variants are complex in nature with apparent maximal enzyme-substrate affinity corresponding to the temperature at which the trout is acclimatized. Both substrates, dl-isocitrate and NADP(+), give similar curves although the magnitude of the K(m) change with temperature is much decreased in the case of NADP(+). 4. E(a) values of approx. 18kcal/mol were determined for both the cold-adapted and warm-adapted enzyme variants. 5. In an attempt to determine how velocities can be increased at low temperatures, cation, pH requirements, metabolite and enzyme concentrations were examined. 6. NAD-isocitrate dehydrogenase could not be detected in trout tissues.


The stimulatory effects of carbon tetrachloride and other halogenoalkanes on peroxidative reactions in rat liver fractions in vitro. General features of the systems used. 1. The general features of the reaction by which carbon tetrachloride stimulates lipid peroxidation have been elucidated in rat liver microsomal suspensions and in mixtures of microsomes plus cell sap. The production of lipid peroxides has been correlated with malonaldehyde production in the systems used. 2. The stimulation of malonaldehyde production by carbon tetrachloride requires a source of reduced NADP(+) and is dependent on the extent of the endogenous peroxidation of the microsomal membranes: if extensive endogenous peroxidation occurs during incubation then no stimulation by carbon tetrachloride is apparent. 3. The stimulation of malonaldehyde production by carbon tetrachloride has been shown to be proportional to the square root of the carbon tetrachloride concentration in the incubation mixture. It is concluded that the stimulation of malonaldehyde production by carbon tetrachloride results from an initiation process that is itself dependent on the homolytic dissociation of carbon tetrachloride to free-radical products. 4. The increased production of malonaldehyde due to carbon tetrachloride is accompanied by a decreased activity of glucose 6-phosphatase in rat liver microsomal suspensions. 5. The relative activities of bromotrichloromethane, fluorotrichloromethane and chloroform have been evaluated in comparison with the effects of carbon tetrachloride in increasing malonaldehyde production and in decreasing glucose 6-phosphatase activity. Bromotrichloromethane was more effective, and fluorotrichloromethane and chloroform were less effective, than carbon tetrachloride in producing these two effects. It is concluded that homolytic bond fission of the halogenomethanes is a requisite for the occurrence of the two effects observed in the endoplasmic reticulum.


The stimulatory effects of carbon tetrachloride on peroxidative reactions in rat liver fractions in vitro. Interaction sites in the endoplasmic reticulum. 1. The actions of various inhibitors of the microsomal NADPH-cytochrome P-450 electron-transport chain have been studied on the stimulatory effect of carbon tetrachloride on malonaldehyde production. 2. Carbon monoxide, p-chloromercuribenzoate, beta-diethylaminoethyl-3,3'-diphenylpropyl acetate (SKF 525A) and nicotinamide did not decrease the stimulatory action of carbon tetrachloride on malonaldehyde production when present in concentrations shown to be capable of strongly inhibiting the demethylation of aminopyrine. 3. In contrast with the effects of the substances mentioned above, low concentrations of cytochrome c strongly depressed the stimulatory action of carbon tetrachloride on malonaldehyde production while increasing the endogenous rate of peroxidation. 4. Aging the microsomal suspensions at 0 degrees C caused a rapid decrease in aminopyrine demethylation activity and in lipid peroxidation catalysed by ADP and Fe(2+). The stimulation of malonaldehyde production by carbon tetrachloride was relatively unaffected, however, by aging the microsomes at 0 degrees C for 3 days; during this period cytochrome P-450 decreased by more than 30%. 5. The conclusion is reached that the interaction between carbon tetrachloride and the NADPH-cytochrome P-450 electron-transport chain necessary for the stimulation of malonaldehyde production involves a locus near to if not identical with the NADPH-cytochrome c reductase flavoprotein.


Importance of the pentose phosphate pathway for D-glucose catabolism in the obligatory aerobic yeast Rhodotorula gracilis. d-Glucose catabolism of a phosphofructokinase-deficient yeast Rhodotorula gracilis has been studied. By using d-glucose specifically (14)C-labelled at different positions and measuring the distribution of the label in various fractions of cell metabolism, the following results were found. 1. The pentose phosphate pathway, being the main pathway of d-glucose catabolism, simultaneously converts glucose molecules into pentose phosphates oxidatively by using two NADP-linked dehydrogenases and via the non-oxidative transketolase-transaldolase pathway. 2. From the correlation of the (14)CO(2) liberation and the d-glucose consumption and from the fact that the pentose phosphate moiety in nucleic acids is almost equally labelled from d-[1-(14)C]- and d-[6-(14)C]-glucose, it is concluded that of the glucose utilized about 80% undergoes transformation via the non-oxidative pentose phosphate pathway. Only about 20% of glucose is directly decarboxylated to pentose phosphate. 3. For further degradation it is postulated that the pentose phosphates are split into C(2) fragments and glyceraldehyde 3-phosphates. 4. All three loci of oxidative decarboxylation appear to be effective in Rh. gracilis, the oxidative part of the pentose phosphate pathway, the decarboxylation of pyruvate in the later part of the glycolytic pathway as well as the oxidation in the tricarboxylic acid cycle. 5. d-Glucose molecules taken up are only partially oxidized to CO(2): about four-fifths of each glucose molecule metabolized is incorporated into cell constituents. 6. The quantitative interrelations of the fluxes of d-glucose subunits along the catabolic pathways have been estimated and are discussed.


Effects of lipid peroxidation on membrane-bound enzymes of the endoplasmic reticulum. 1. Induction of the formation of lipid peroxide in suspensions of liver microsomal preparations by incubation with ascorbate or NADPH, or by treatment with ionizing radiation, leads to a marked decrease of the activity of glucose 6-phosphatase. 2. The effect of peroxidation can be imitated by treating microsomal suspensions with detergents such as deoxycholate or with phospholipases. 3. The substrate, glucose 6-phosphate, protects the glucose 6-phosphatase activity of microsomal preparations against peroxidation or detergents. 4. The loss of glucose 6-phosphatase activity is not due to the formation of hydroperoxide or formation of malonaldehyde or other breakdown products of peroxidation, all of which are not toxic to the enzyme. 5. All experiments lead to the conclusion that the loss of activity of glucose 6-phosphatase resulting from peroxidation is a consequence of loss of membrane structure essential for the activity of the enzyme. 6. In addition to glucose 6-phosphatase, oxidative demethylation of aminopyrine or p-chloro-N-methylaniline, hydroxylation of aniline, NADPH oxidation and menadione-dependent NADPH oxidation are also strongly inhibited by peroxidation. However, another group of enzymes separated with the microsomal fraction, including NAD(+)/NADP(+) glycohydrolase, adenosine triphosphatase, esterase and NADH-cytochrome c reductase are not inactivated by peroxidation. This group is not readily inactivated by treatment with detergents. 7. Lipid peroxidation, by controlling membrane integrity, may exert a regulating effect on the oxidative metabolism and carbohydrate metabolism of the endoplasmic reticulum in vivo.


Pneumonia.

Pneumonia.


A system for the study in situ of the pentose phosphate pathway in rabbit liver. A surgical procedure for the isolation of the liver from the systemic circulation of the anaesthetized rabbit is described. The technique allowed the metabolism in situ of intraportally infused substrates to be followed for periods up to 5min, free from the contaminating influences of metabolism by other body tissues. Details of the procedures necessary to achieve the uniform infusion, homogeneous distribution and containment of (14)C-labelled glucose substrates in the liver by haemostasis are described. Changes in pO(2), pCO(2), pH and the concentrations of NADP(+), NADPH and glucose during each minute interval of the total 5min period of metabolism are given. Reactant ratios of the lactate dehydrogenase system and the adenine nucleotide system have been calculated from the concentrations of the pertinent metabolites for the same period of metabolism. Glucose production by rabbit liver in situ proceeded at the rate of 1.08mumol/min per g wet wt. of liver during the 5min metabolic interval. The presence of the oxidative reactions of the pentose phosphate pathway of glucose metabolism was inferred from the quotient oxidation of [1-(14)C]glucose/oxidation of [6-(14)C]glucose=1.8.


Supraventricular tachycardia. I.

Supraventricular tachycardia. I.


Supraventricular tachycardia. II.

Supraventricular tachycardia. II.


Effect of isoprenaline on cells in different phases of the mitotic cycle.


Angiotensin II-like activity in circulating arterial blood in immature and adult rabbits. 1. Angiotensin II-like activity was estimated from the contraction of the rat ascending colon superfused with arterial blood in an extracorporeal circuit. The colon was calibrated by infusion of synthetic angiotensin II amide (Hypertensin, CIBA Ltd).2. Resting levels of angiotensin II-like activity were obtained by comparison of the length of a rat colon preparation superfused alternately with blood from an intact and from a nephrectomized rabbit.3. The resting levels of angiotensin II-like activity reached a maximum value of about 1.6 ng/ml. at 10-14 days of age, and thereafter declined to the adult value of 0.23 ng/ml. Rabbits of less than 100 g body weight had resting levels in the same range as adults.4. Removal of 25% of the calculated blood volume induced five times greater increments in arterial angiotensin II-like activity in immature than in adult rabbits (1.68 +/- 0.26 S.E. of mean ng/ml. compared with 0.34 +/- 0.08 S.E. of mean ng/ml.).5. No evidence was obtained that the high levels of angiotensin II-like activity encountered in immature rabbits were attributable to a slower rate of inactivation of angiotensin by tissues.6. The pronounced activity of the renin-angiotensin system demonstrated by these experiments in immature rabbits especially during haemorrhage is consistent with the suggestion that this system is of particular importance in cardiovascular control in early life.


Inhibition by prostaglandin E 1  of gastric secretion in the dog. 1. The effect of prostaglandin E(1) (PGE(1)) on gastric secretion was studied in dogs equipped with gastric fundic pouches, either innervated (Pavlov) or denervated (Heidenhain).2. PGE(1) inhibited gastric secretion (volume, acid concentration, acid output, pepsin output) when given either by constant intravenous infusion or by single intravenous injection. The degree of inhibition was dose dependent.3. The antisecretory effect of PGE(1) was demonstrated against gastric stimulants which operate through different mechanisms. Thus, PGE(1) counteracted the secretogogue effect of:(a) histamine dihydrochloride; the ED(50) was 0.5-1.0 mug/kg. min for a submaximal dose, and 1.0-1.5 mug/kg. min for a maximal dose;(b) pentagastrin; the ED(50) was around 0.25 mug/kg. min;(c) food; the ED(50) was 0.5 to 0.75 mug/kg. min;(d) 2-deoxyglucose; the ED(50) was less than 0.1 mug/kg. min.4. Although in some experiments, nausea and vomiting were observed during administration of PGE(1), the antisecretory property of the substance is not related to a vomiting reflex, since(a) an antiemetic, such as atropine, prevented vomiting without interfering with the effect of PGE(1), and(b) profuse vomiting elicited by apomorphine did not reduce gastric secretion stimulated by either histamine or pentagastrin.5. The mechanism by which PGE(1) inhibits gastric secretion is unknown. Studies by others have shown that the compound reduces gastric mucosal blood flow, inhibits acid formation from gastric mucosa when applied in vitro and may change the rate of formation of gastric cyclic AMP. It is likely that PGE(1) interferes with biochemical processes, within parietal and chief cells, which lead to elaboration of gastric juice.6. Unlike most gastric inhibitors, PGE(1) appears to act as a protective shield against most, if not all, gastric stimulants. Since prostaglandins of the E series are naturally occurring substances and are normally present in the stomach, they may play a role in the regulation of gastric secretion.


Simple memory: a theory for archicortex.


Abnormal autolytic enzyme in a pneumococus with altered teichoic acid composition. Pneumococci in which the choline component of the cell wall teichoic acid was replaced by ethanolamine contain an abnormal autolytic enzyme that has a low molecular weight and low activity in contrast to the enzyme typical of choline-containing bacteria that has a high molecular weight and high activity. The abnormal autolysin can be converted to the normal (cholinetype) enzyme by incubation in vitro with choline-containing cell walls.


Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone. After incubation with thymosin, a thymic hormone, normal bone marrow rosette-forming cells acquire T-cell characteristics, including increased sensitivity to azathioprine, anti-lymphocyte serum, and anti-theta serum. This activity of thymosin provides a new sensitive and reproducible bioassay for thymosin, and is well correlated with an in vivo graft-versus-host assay. In addition, incubation of spleen cells from adult thymectomized mice with thymosin in vitro restores to normal their diminished sensitivity to azathioprine and anti-lymphocte serum.


Increases in tyrosine hydroxylase activity after exposure to cyclopropane and fluroxene.


The effect of sex hormones on the activity of tryptophan oxygenase and other corticosteroid-inducible enzymes in rat liver.


Comparative aspects of spermatogenesis.

Comparative aspects of spermatogenesis.


Interaction between lymphocytes in immune responses.


Enzyme activities in the anterior horn of spinal cord after  , -iminodipropionitrile.


Biosynthesis of myo-inositol by rat testis following surgically induced cryptorchidism or treatment with triethylenemelamine.


The response of normal men and women to changes in their environmental temperatures and ways of life.


A comparison of the cardiorespiratory effects of salbutamol and isoproterenol-phenylephrine aerosols in asthma.


Humoral regulators in the development and progression of leukemia.


Biochemistry.

Biochemistry.


The functional difference between the monomer and the dimer of D-amino acid oxidase.


S-ureidoglycolate dehydrogenase: purification and properties.


Reduction of alkyl hydroperoxides to alcohols: role of rubredoxin, an electron carrier in the bacterial hydroxylation of hydrocarbons.


Developmental hormones and immunological maturation.


Nutrition.

Nutrition.


Conferment of immunocompetence on lymphoid cells by a thymic humoral factor.


Thymosin, a thymic hormone influencing lymphoid cell immunological competence.


Synergism between cortisol and antilymphocyte serum. I. Observations in murine allograft systems.


The effect of adrenergic receptor blockade on the exercise-induced serum growth hormone rise in normals and juvenile diabetics.


Effect of cold on TSH secretion in man.

Effect of cold on TSH secretion in man.


Testosterone biosynthesis in the desert mouse, Acomys cahirinus.


[Six years' experience with a musculoplastic technic].


Differential effect of 5-bromodeoxyuridine on the concentrations of specific enzymes in hepatoma cells in culture. Growth of cultured rat hepatoma cells in the presence of 5-bromodeoxyuridine results in a rapid inhibition of the synthesis of adrenal steroid-inducible tyrosine aminotransferase (EC 2.6.1.5) and slower decreases in the concentrations of lactate dehydrogenase (EC 1.1.1.27), alcohol dehydrogenase (EC.1.1.1.1), and glucose-6-phosphate dehydrogenase (EC 1.1.1.49). During the same period, neither overall cell growth nor the concentrations of malate dehydrogenase (EC 1.1.1.37), acid phosphatase (EC 3.1.3.2), or alanine aminotransferase (EC 2.6.1.2) were significantly decreased by the base analog. Addition of thymidine to the growth medium rapidly counteracts the inhibition of tyrosine aminotransferase synthesis but restores the normal concentrations of lactate-, alcohol-, and glucose-6-phosphate dehydrogenases much more slowly. Growth of the cells for only one generation in the presence of bromodeoxyuridine, followed by the addition of thymidine, produces transient decreases in the concentrations of the three "late-responding" dehydrogenases, beginning 2-3 generations after exposure to the analog.It is concluded that the selective inhibitory effects of the analog could result from a mechanism in which bromodeoxyuridine is uniformly incorporated into cellular DNA, but inhibits the transcription of only certain genes into messenger RNA. A mathematical model is derived to account for the observed differences in the kinetics of the inhibition of synthesis of the gene products that are sensitive to the analog.


The effect of presensitization of parental donors on graft-versus-host disease in irradiated F 1 hybrid mice.


Growth of the blue-green algae virus LPP-1 under conditions which impair photosynthesis.


Value of electrical pacing and beta adrenergic blockers in the prevention of fatal ventricular arrhythmias.


Metabolic regulation by chemical modification of enzymes.


Biochemistry of catecholamines.

Biochemistry of catecholamines.


Effect of cycloheximide on the ultrastructural changes produced by spironolactone and pregnenolone-16 alpha-carbonitrile in the rat liver.


Protein-protein interaction and enzymatic activity.


Paradoxical refractoriness to induction by cortisone following a peculiar adaptation of liver enzymes by bentonite or celite.


Enzymatic conversion of 16-hydroxypalmitic acid into 10,16-dihydroxypalmitic acid in Vicia faba epidermal extracts.


Increased levels of pisatin and phenylalanine ammonia lyase activity in Pisum sativum treated with antihistaminic, antiviral, antimalarial, tranquilizing, or other drugs.


On the accuracy of clinical judgements.

On the accuracy of clinical judgements.


Effects of intraarterial catecholamine infusions on blood flow in the canine gut.


The glucose effect and cortisone action upon rat liver metabolism.


Therapy of barbiturate overdose.

Therapy of barbiturate overdose.


What you should know about the Illinois Controlled Substances Act.


Acute glomerulonephritis in the elderly.


Combined treatment of Parkinsonian tremor with propranolol and levodopa.


Different molecular forms of bovine adrenal tyrosine hydroxylase.


Mitochondrial protein synthesis: inhibition by emetine hydrochloride.


Inhibition of a reduced nicotinamide adenine dinucleotide phosphate-linked aldehyde reductase from bovine brain by barbiturates.


Periarteritis nodosa, Australia antigen and lymphatic leukemia.


Vasodilatation induced by streptomycin in the perfused canine kidney.


Antibiotics and ureter. VI. Aminosidin.

Antibiotics and ureter. VI. Aminosidin.


The comparative pharmacology of WG253 (rimiterol hydrobromide), a new bronchodilator.


Drug biotransformation in the human fetus: nitro group reduction.


Hyperproduction and purification of nicotinamide deamidase, a microconstitutive enzyme of Escherichia coli.


The generation of superoixide radical during the autoxidation of ferredoxins.


Neural redundancy and regularity of swimming beats in scyphozoan medusae.


Studies on the mechanism of action of D-amino acid oxidase. Evidence for removal of substrate  -hydrogen as a proton.


Second International Symposium on Gentamicin. An aminoglycoside antibiotic.


Effect of force-feeding diets lacking leucine, valine, isoleucine, threonine or methionine on amino acid catabolism in rats.


Metabolic fate of 1,3-butanediol in the rat: conversion to  -hydroxybutyrate.


The application of nuclear magnetic resonance to pharmacological problems.


The induction of long-term immunological tolerance.


A potent beta-adrenoreceptor blocking drug: 4-(2-hydroxy-3-isopropylaminopropoxy)indole.


The influence of corticosteroid pretreatment on anaphylactic bronchoconstriction in the guinea-pig.


A procedure for the analysis of illicit diamorphine samples.


Plasma protein binding of chlorpromazine.


The activities of alpha- and beta-adrenoceptive blocking agents in reducing intestinal relaxation due to sympathetic stimulation in the pithed rat.


A note on the determination of dichlorophen and hexachlorophene in mixtures.


Sequential blockade of the mycobacterial de novo folate pathway. A review.


The use of boronate derivatives in the characterization of cathecholamines and related beta-hydroxy-amines by gas liquid chromatography-mass spectrometry.


Assistance for the physician: nurse clinician or physician assistant.


The absolute configuration of trans-2-dimethylaminocyclohexyl acetate methiodide: chemical verification.


1,2-disubstituted cyclohexanes as substrates of acetylcholinesterase and muscarinic agents. A re-investigation.


Determination of pharmacokinetic parameters for urinary excretion of sulphafurazole under normal and controlled alkaline urine conditions.


Plasma protein binding of tricyclic antidepressive drugs.


The release and detection of bronchoactive substances by serially perfused isolated lungs.


Effects of two cholinesterase inhibitors on acetylcholine release from the guinea-pig isolated ileum preparation.


Recovery of the amine uptake-storage mechanism in nerve granules after reserpine treatment: inhibition by axotomy.


Cardiac catecholamine levels and blood pressure after chronic treatment with beta-adrenergic blocking agents.


The role of brain catecholamines in morphine analgesic action in morphine tolerant rats.


Central nervous system stimulant action of fenfluramine in rabbits.


Multiple-drop formation in emulsions.

Multiple-drop formation in emulsions.