A computer-controlled scraper for standard thin-layer plates.


Quantitative thin-layer chromatography. Assay of drug mixtures by scanning of remission peaks.


Effect of humidity on the amount of fluorescence exhibited by chromatographically separated dansyl derivatives.


Solid phase synthesis and purification of a pentapeptide.


High-voltage electrophoresis.

High-voltage electrophoresis.


Porphyrias. Separation and identification by high-voltage electrophoresis.


The use of high-voltage paper electrophoresis in studies of the biosynthesis of mucin glycoproteins.


An improved automatic lipid extraction and thin-layer spot application system.


Microanalytical quantitation of serum lipids by thin-layer chromatography utilizing an automated applicator.


The quantitative assessment of chromatographically separated chromogens and fluorogens.


Identification and quantitation of amines by thin-layer chromatography.


Planning the duration of long-term survival time studies designed for accrual by cohorts.


Sequence of atrial excitation in the dog during antegrade and retrograde activation.


Social trauma in the epidemiology of cancer of the cervix.


The use of intermittent catheterization in a bladder training program: preliminary report.


Derivation of aimed electrocardiographic leads from the multiple expansion.


Permanent pervenous atrial pacing.

Permanent pervenous atrial pacing.


Electrocardiographic parameters of the normal domestic cat: a comparison of standard limb leads and an orthogonal system.


Vectorcardiographic features of left anterior hemiblock combined with complete right bundle branch block.


A statistical analysis of atrial arrhythmia in A-V block.


Stationary television display of analog events--an aid in cardiovascular teaching.


Atrial tachycardia with high degree of intra-atrial block.


Unusual normalization of the electrocardiogram on the 6th day of myocardial infarction.


Differential vectorcardiography.

Differential vectorcardiography.


Electrocardiographic changes simulating hypokalemia during treatment with lithium carbonate.


Quantitative interactions of feline leukaemia virus and its pseudotype of murine sarcoma virus in cat cells: requirement for DNA synthesis.


Complementation and recombination with temperature-sensitive mutants of adenovirus type 5.


Characteristics of a group IV cholera phage.


Pyloric armature of new world phlebotomine sandflies (Diptera, Psychodidae).


Xenarthronyssus furmani, n. gen., n. sp. (Acarina: Dasyponyssidae), parasites of armadillos, with two subspecies.


Dermacentor variabilis (Say) (Acarina: Ixodidae) established in southeastern Washington and northern Idaho.


A survey of bat ectoparasites in West Malaysia.


On Lutzomyia flaviscutellata (Mangabeira) and L. Olmeca (Vargas and Diaz-Najera) (Diptera: Psychodidae).


Effects of ultra-low volume applications of malathion in Hale County, Texas. 3. Effect on nontarget animals.


Seasonal parous rates in Hybomitra lasiophthalma (Diptera: Tabanidae).


Differentiation of Hippelates pusio, H. Bishoppi and H. pallipes (Diptera: Chloropidae) by electrophoresis.


Effect of polyinosinic-polycytidylic acid on induction of primary or transplanted tumors by chemical carcinogen or irradiation.


Nitrosamine studies: induction of lung adenomas by concurrent administration of sodium nitrite and secondary amines in Swiss mice.


Cocarcinogenesis studies on mouse skin and inhibition of tumor induction.


Unusually large tumor in a Sydney rock oyster.


Sunlight exposure and risk of developing cutaneous and oral squamous cell carcinomas in white cats.


Tumors of the urinary bladder: an analysis of the occupations of 1,030 patients in Leeds, England.


Gallium-67 localization in lysosomal-like granules of leukemic and nonleukemic murine tissues.


Host resistance and radiation response of syngeneic mouse lymphoma cells.


Skin tumors in mice painted with N-methyl-N'-nitro-N-nitrosoguanidine and N-ethyl-N'-nitro-N-nitrosoguanidine.


Evolution of cell-mediated immunity in mice bearing tumors produced by a mammary carcinoma cell line. Influence of tumor growth, surgical removal, and treatment with irradiated tumor cells.


Asbestos cancers.

Asbestos cancers.


Vertebral chordomas.

Vertebral chordomas.


Tuberculous atlanto-axial dislocation (with remarks on the mechanism of dislocation).


White matter destruction in a case of spontaneous cerebrospinal fluid rhinorrhoea.


Diagnosis and management of neurogenic bladder. An analysis of 25 cases.


Hypothyroid hypertrophic myopathy. A clinical and histochemical study.


Intradural avulsion of dorsal nerve root.


Internal 'ophthalmoplegia' in myasthenia gravis.


Vitelliform macular degeneration.

Vitelliform macular degeneration.


Our results with the operation of ab externo trabeculotomy.


Experience with the examination of the chamber angle by means of the routine ophthalmic loupe. Contribution to the lens-free examination.


The electroretinogram and the visual evoked potential in two patients with tuberculum sellae meningioma before and after decompression of the optic nerve.


Four new leptospiral serotypes isolated from human sources in South Vietnam.


Babesia gibsoni: influence of infection on serum enzymes of dogs.


Experimental cholera produced by Vibrio cholerae and choleragenic filtrates: light and electron microscopic findings in the small intestine.


A preliminary study of chemotherapy of Gnathostoma spinigerum infections in cats with ancylol disophenol (2,6-diiodo-4-nitrophenol).


Treatment of A, lumbricoides with a small dose of pyrantel pamoate (combantrin).


Plasma amino acids in Kwashiorkor and marasmus in Thailand.


Epidemiological study of Schistosomiasis japonica on Khong Island, southern Laos.


Plague meningitis in infants.

Plague meningitis in infants.


The histopathology of maedi a slow, viral pneumonia of sheep.


Choroid plexus papilloma in a dog.

Choroid plexus papilloma in a dog.


Proliferative lesions of serous membranes in ovariectomised female and entire male dogs after stilboestrol administration. This paper reports the effects of prolonged stilbestrol administration in ovariectomized female dogs and some observations on the effects of this hormone on a group of male dogs.  It was shown previously that ovarian tumors, which frequently metastasise to serous surfaces, can be produced in dogs by prolonged administration of stilbestrol.  Total amounts of stilbestrol given to individual dogs in this study ranged from 180 to 570 mg, mainly over a period of 48 weeks.  The ability of non-ovarian serous surfaces to proliferate in response to stilbestrol, independently of ovarian metastases, was studied in 5 females and 4 males.  Papillary proliferations occurred on the uterine serosae in 3 females and on the testicular and epididymal serosae in 2 males.  In no case was there papillary proliferation on serous membranes of non-genital organs.  One of 2 entire females given stilbestrol had proliferations regarded as metastases of ovarian origin on non-genital serous membrane.  In addition to ovarian changes, stilbestrol can cause primary proliferative lesions in the serous membranes of certain genital organs in both sexes.  There is no evidence that serous membranes of non-genital organs be capable of a primary proliferative response to stilbestrol.


An ovarian tumour in a southern elephant seal (Mirounga leonina).


Interrelationships between riboflavin and dietary energy and protein utilization in growing chicks.


Evaluation of nitrogen metabolites as indices of nitrogen utilization in sheep given frozen and dry mature herbages.


The nutrition of the veal calf. 4. The effect of offering roughage on health and performance.


The effect of environmental temperature on the performance and health of the pre-ruminant and ruminant calf.


Proposal to use time spent chewing as an index of the extent to which diets for ruminants possess the physical property of fibrousness characteristic of roughages.


Nutritional aspects of amino acid metabolism. 1. A rat liver perfusion method for the study of amino acid metabolism.


The retention of ascorbic acid by guinea-pig tissues.


Body iron and its distribution between haem and non-haem fractions in the suckling rat.


The effect of tertiary branched-chain carboxylic acids on the energy metabolism of sheep.


Digestion of concentrates in sheep. 3. Effects of rumen fermentation of barley and maize diets on protein digestion.


Level of food intake and the passage of markers and nitrogen along the alimentary tract of sheep.


The effect of keratinisation of the respiratory metabolism of epitheliomas in DMBA-treated mice skin.


Impairment of humoral antibody response in neonatally thymectomized and irradiated chickens.


Effect of phytohemagglutinin on thymidine incorporation and antibody production in the secondary response in vitro towards a defined hapten.


Suppression of experimental allergic encephalomyelitis by a synthetic polypeptide.


Delayed hypersensitivity to azobenzenearsonate-N-acetyl-L-tyrosine. In vivo and in vitro study.


Synthesis of a radioiodine-labeled 2,4-dinitrophenyl-hapten and its use for binding assays.


The effect of carrier-primed cells on an adoptive anti-hapten response.


In vitro studies on the release of mitogenic factor by sensitized human lymphocytes.


The antibody response of pigs to dinitrophenylated bovine IgG administered with a novel type of adjuvant.


Reconstitution of a thymus cell-deprived immune system by syngeneic and allogeneic thymocytes in vitro.


Formation and function of a complement-activating enzyme generated from factors of guinea pig serum and cobra venom.


Puff induction and regression in Rhynchosciara angelae by the method of salivary gland implantation.


Molecular interactions and solubility of polar nonelectrolytes in nonpolar solvents.


Membrane diffusion. II. Influence of physical adsorption on molecular flux through heterogeneous dimethylpolysiloxane barriers.


Physiological disposition of fenoprofen in man. I. Pharmacokinetic comparison of calcium and sodium salts administered orally.


Prediction of stability in pharmaceutical preparations. XVI. Kinetics of hydrolysis of canrenone and lactonization of canrenoic acid.


Enamine prodrugs.

Enamine prodrugs.


Inhibited dissolution of drug crystals by certified water-soluble dyes. 3.


Drug transport. V. Mechanism of potassium-ion inhibition of passive transfer of solutes across everted rat intestine.


Binding study of p-hydroxybenzoic acid esters to bovine serum albumin by fluorescent probe technique.


Influence of HLB values of surfactants on ephedrine release from emulsified liquid systems.


Normal and promoted GI absorption of water-soluble substances. 3. Absorption of antibiotics from stomach and intestine of the rat.


Pharmacological properties of hypobranchial gland of Thais haemastoma (Clench).


Metabolism of the radioprotective agent triammonium 2-dithiocarbamyl-3-dithiocarbonylthiopropanoate in the mouse.


Nonaqueous titration of barbiturates in tetramethylurea.


Colorimetric assay procedure for dissolution studies of meprobamate formulations.


Coulometric titration of N-isopropyl- -(2-methylhydrazio)-p-toluamide hydrochloride.


Analytical applications of p-nitrosobenzoic acid. I. Specific colorimetric determination of m-aminophenol in p-aminosalicylic acid and sodium p-aminosalicylate.


Elimination of choline interference in microbiological assay of inositol in pharmaceutical products.


Automated system for analytical microbiology. 3. Computer interpolation of potency.


Semiautomated fluorometric determination of rauwolfia alkaloids.


Effect of particle size and speed of compaction on density changes in tablets of crystalline and spray-dried lactose.


Batch production of pharmaceutical granulations in a fluidized bed. I. Effects of process variables on physical properties of final granulation.


Influence of fluid motion in dissolution-rate determinations.


Opium alkaloids. XI. Biosynthesis of aporphines in Papaver somniferum.


Liquid crystalline phases in aerosol formulations. I. Phase equilibria in propellant compositions.


Identity of a major chromogen in p-aminosalicylic acid and sodium p-aminosalicylate dosage forms.


Photodegradation products of chloramphenicol in aqueous solution.


Chemical constituents of Cannabis sativa L. root.


Direct measurement of aspirin.

Direct measurement of aspirin.


Contact angles and wettability of human skin.


Reduced side effects following administration of a new anorectic agent, 4'-chloro-2-(ethylamino)propiophenone, in timed-release form.


New compounds: anils.

New compounds: anils.


New compounds: synthesis of dimethyl N,N'-bis(2-amino-3-oxophenoxazine-4,6-dimethyl-1,9-dicarbonyl)-dl-dialaninate.


Novel behavior of N-methyl-3-benzoyl-3-hydroxypiperidine under conditions of Von Braun demethylation.


Efficient deoxygenation of water by gas permeation.


Strontium-90 in human vertebrae in Czechoslovakia, 1964-1969.


Results of a survey of x radiation from color television receivers in the metropolitan area of San Juan, Puerto Rico, 1969-1970.


Gross radioactivity in surface waters of the United States, March 1971.


Radioactivity in airborne particulates and precipitation.


Further studies on the experimental infection of the mouse with trachoma agents.


Results of treatment of trachoma with vaccine and long-acting sulphonamide combinations compared with the vaccine and the sulphonamide alone.


Comprehensive trachoma control program among the southwestern American Indians.


The use of testosterone in bilateral nephrectomized dialysis patients.


Measurement of dialysance and bi-directional fluxes of calcium in vivo using radiocalcium.


Plastics with a stable surface monolayer of cross-linked heparin: preparation and evaluation.


A controlled evaluation of clinical and metabolic effects of dialysate calcium levels during regular hemodialysis.


An artificial urethral sphincter.

An artificial urethral sphincter.


Bladder regeneration after cystectomy and prosthetic urinary bladder replacement.


Implantable artificial sphincter ani.

Implantable artificial sphincter ani.


The combined use of balloon diastolic augmentation and mechanical ventricular assistance.


Automatic control of intra-aortic balloon pumping.


Surface charge behavior of pyrolytic carbon in saline and blood plasma.


Proximity to aortic valve and unidirectionality as prime factors in counterpulsation effectiveness.


Fault detection for the intra-aortic balloon using acoustic diagnostics.


Effects of change of ambient pressure differential on the effectiveness of peripheral external assist.


Prolonged circulatory support by direct mechanical ventricular assistance for two to three days of ventricular fibrillation.


A new method of pulsatile bypass without heparin for aortic arch replacement.


Hematologic observations during prolonged partial left ventricular bypass.


Pathophysiologic studies on prolonged continuous pumping of series-type left heart assist device in calves.


The urokinase-heparin bonded synthetic surface. An approach to the creation of a prosthetic surface possessing composite antithrombogenic and thrombolytic properties.


The development of an automatically controlled intrathoracic total left-heart bypass system.


Total body extracorporeal pumping with pulsatility.


Inertial cardiac assistance.

Inertial cardiac assistance.


Heat-induced myocardial angiogenesis, I.


Coated adsorbents for direct blood perfusion: HEMA-activated carbon.


Removal of uremic waste metabolites from the intestinal tract by encapsulated carbon and oxidized starch.


The importance of zeta potential, ultrastructure, and electrical conductivity to the in vivo performance of polyurethane-carbon black vascular prostheses.


Possible uremic detoxification via oral-ingested microcapsules.


Clinical evaluation of chronic, intermittent, and short term hemoperfusions in patients with chronic renal failure using semipermeable microcapsules (artificial cells) formed from membrane-coated activated charcoal.


Blood cleansing by diafiltration in uremic dog and man.


Clinical maintenance hemodialysis with a sorbent-based, low-volume dialysate regeneration system.


Use of fluorochemicals for renal perfusion.


Asymmetric hollow fiber membranes for dialysis.


Clinical evaluation of the disc cone dialyzer.


Transfer of chemical species through a protein gel.


Optimization of collagen dialysis membranes.


New pacemaker electrodes.

New pacemaker electrodes.


An experimental self-sealing prosthesis for hemodialysis.


The use of mathematical and physical models for optimization of oxygenators.


Oxygen transfer to blood: data and theory.


Predictive correlation of oxygen and carbon dioxide transfer in a blood oxygenator with induced secondary flows.


Oxygen transfer to blood in membrane lungs: correlation with and prediction from in vivo measurements.


Development and evaluation of a new hollow-fiber membrane oxygenator.


The in vitro characteristics of a toroidal flow membrane oxygenator.


Extended term (to 16 days) partial extracorporeal blood gas exchange with the spiral membrane lung in unanesthetized lambs.


Clinical cardiopulmonary dynamics during prolonged extracorporeal circulation for acute respiratory insufficiency.


Observations of acute thrombogenesis in membrane oxygenators.


The use of a membrane oxygenator for treatment of respiratory insufficiency.


Methods to construct artificial organs.

Methods to construct artificial organs.


An automatic cardiopulmonary bypass unit for use in infants.


Moving computer graphic images seen from inside the vascular system.


Applications of ultra-miniature photoelectric plethysmographic sensors with a very short response time to the non-traumatic study of the circulatory system.


Erythrocyte metabolism following surface induced injury.


Ultrahigh power electromagnetic energy transport into the body.


An artificial heart with independently variable pulse parameters.


Isolated intestinal perfusion: a method for assessing preservation methods and viability before transplantation.


Can we achieve over 100 hours' survival with a total mechanical heart?


Problems in total artificial heart.

Problems in total artificial heart.


The development and implantation of a polyurethane hemispherical artificial heart.


Characteristics of the human heart: design requirements for replacement.


Artificial heart with hemispherical ventricles II and disseminated intravascular coagulation.


Artificial heart constructed with biological material.


Radially supported membrane valves implanted in the pulmonary artery of calves.


Comparative turbulence of heart valves (natural and prosthetic).


ASAIO--our society.

ASAIO--our society.


Biolized materials for cardiovascular prosthesis.


Effect of hemodialysis on the inhibition of nervous tissue transketolase and on uremic neuropathy.


The genesis of the square meter-hour hypothesis.


Assay of serum abnormalities in uremic and dialysis patients. Evidence for depletion of vital substances in hemodialysis.


Bait shyness of two gerbils, Tatera indica Hardwicke and Meriones hurrianae Jerdon.


[Hepatitis associated antigen in cases of banal and chronic hepatitis and in cases of cirrhosis].


Scanning electron microscopy: a new tool for physiologic and pathophysiologic study of the digestive tract.


Perfusion of the isolated dog pancreas. A quantitative ultrastructural study of the spontaneous exocrine secretion.


Effect of glucagon on ventricular arrhythmias after coronary artery occlusion and on ventricular automaticity in the dog. 1. In anaesthetized dogs, glucagon (100 mug/kg i.v.), caused a significant increase in heart rate and decrease in mean arterial blood pressure. Ventricular automaticity, as determined by the time to the onset of first vagal escape beat and the number of such indioventricular beats during the 30 s period of vagal stimulation, was not significantly altered.2. In unanaesthetized dogs with ventricular arrhythmias produced by two-stage ligation of the anterior descending branch of the left coronary artery, glucagon (30 and 100 mug/kg i.v.), restored normal sinus rhythm in a few animals. In the remaining dogs, there was a significant reduction in the ventricular ectopic activity.3. The significant positive chronotropic response to glucagon elicited in anaesthetized animals was not observed in conscious dogs whose coronary arteries had been ligated.4. These findings enhance the potential usefulness of glucagon in the treatment of acute myocardial infarction, which may often be associated with disturbances of ventricular rhythm.5. In the light of observations made by other workers, it is suggested that the antiarrhythmic effect of glucagon may be due to movement of potassium ions into the cardiac cell.


A new method of destroying adrenergic nerves in adult animals using guanethidine. 1. The structure of sympathetic neurones in the rat has been examined with histological, fluorescence histochemical and electron microscopical methods after chronic treatment for 6 weeks with guanethidine (25 or 30 mg/kg/day i.p.).2. Less than 2% of the nerve cell bodies in the superior cervical ganglion remained at this time and in these cells the mitochondria were badly damaged. Few fluorescent adrenergic nerve fibres were found outside the central nervous system. This situation persisted even 4 months (the longest period studied) after cessation of treatment.3. This procedure is proposed as a new method of producing sympathectomy. It has the advantage of being applicable to adult animals in a variety of experimental and pathological situations. It is uniquely advantageous for denervation of the male reproductive tract.


Drug displacement from protein binding: isolation of a redistributional drug interaction in vivo. 1. The pharmacokinetic interaction between phenylbutazone and sulphadoxine has been studied in live sheep.2. Intravenous injection of phenylbutazone causes a rapid fall in the concentration of total sulphadoxine together with a simultaneous rise in the concentration of free sulphadoxine in plasma. Both processes are complete within 3 min of the injection of phenylbutazone.3. These reciprocal changes in the concentrations of total and free sulphadoxine in plasma do not depend on changes in the absorption, hepatic metabolism or renal excretion of sulphadoxine. By exclusion therefore, it is concluded that the effect of phenylbutazone in this interaction is solely to cause redistribution of the sulphadoxine.


Drug displacement from protein binding: source of the sulphadoxine liberated by phenylbutazone. 1. A quantitative study has been made of the redistribution of sulphadoxine produced by phenylbutazone in live sheep.2. From calculations based on plasma measurements, 73% of the sulphadoxine in the body can be accounted for as the sum of the plasma-bound, extracellular-free and intracellular-free drug in the whole animal. This sum is termed the accountable sulphadoxine.3. After the injection of phenylbutazone there was a large shift of sulphadoxine from the plasma-bound form into the free form. The gain in free drug significantly exceeded the loss of bound sulphadoxine from the intravascular compartment, but the discrepancy could easily be explained by displacement of some bound sulphadoxine from extravascular plasma protein.4. Phenylbutazone did not displace sulphadoxine from sheep erythrocytes in vitro, nor from homogenates of liver, kidney or skeletal muscle.5. Although the existence of other susceptible binding sites cannot be entirely excluded, this evidence strongly suggests that all the sulphadoxine liberated by phenylbutazone comes from binding sites on plasma protein.


Chloroquine concentrations in the skin of rabbits and man. 1. Chloroquine was given by a single intravenous injection to rabbits and to two groups of patients. The concentrations of the drug and its more polar metabolites in plasma and tissue were measured by a fluorometric method.2. The concentrations of chloroquine in rabbit liver exceeded those in skin and these in turn exceeded those in plasma. Chloroquine concentrations in rabbit skin were fairly steady between 24 and 72 h after injection. Chloroquine was still present in skin 30 days after the injection. Metabolites of chloroquine were present in plasma, skin and liver.3. Skin was taken from patients 48 h after an intravenous dose of chloroquine (1.25 mg/kg). Skin from patients prone to chloroquine-induced pruritus contained higher concentrations of chloroquine (P<0.01) and lower concentrations of metabolites (P<0.01) than skin from other patients.4. It is suggested that increased liability to chloroquine-induced pruritus may be associated with a lower rate of chloroquine metabolism.


Nicotinic stimulant action of some tolyl and xylyl analogues of 1,1-dimethyl-4-phenylpiperazinium (DMPP). 1. The nicotinic stimulant properties of DMPP have been compared with those of its ortho-, meta- and para-tolyl analogues, and its 2,6- and 3,5-xylyl analogues, on the blood pressure of the cat and on the semispinalis cervicis muscle of the chick.2. A single ortho-methyl group does not significantly change the nicotinic activity, but two such groups greatly reduce or abolish activity. Meta- or para-methyl substitution reduces activity, but does not abolish it.3. The effect on nicotinic activity of methyl substitution in the phenyl ring of DMPP closely parallels the effect of ring-methyl substitution in phenyl choline ether.4. Ortho-methyl substitution has been shown spectroscopically to alter markedly the spatial orientation of the phenyl ring of DMPP relative to the piperazine ring. The effect of these conformational changes on nicotinic activity is discussed in relation to a recent hypothesis concerning the conformation of acetylcholine required for interaction with nicotinic receptors.5. The compounds tested had no muscarinic activity on the isolated guinea-pig ileum.


Mechanism of potentiation of contractor responses to catecholamines by methylxanthines in aortic strips. 1. Caffeine and theophylline increased the amplitude of contractor esponses of untreated and reserpine pretreated rabbit aortic strips to catecholamines (adrenaline, alpha-methylnoradrenaline, noradrenaline). Responses to amines without both the 3- and 4-OH groups in the benzene ring (methoxamine, phenylephrine, Synephrine) were not increased by theophylline and only those to Synephrine were slightly enhanced by caffeine.2. Compounds which inhibit catechol-O-methyltransferase (pyrogallol, tropolone, U-0521) potentiated responses to catecholamines and abolished the enhancing effect of theophylline and caffeine. Also, the potentiation produced by inhibitors of O-methylation was significantly reduced in the presence of the methylxanthines.3. Experiments done with the aid of the technique of oil immersion, to eliminate the diffusion of drug from the tissue into the bathing medium, showed that theophylline and caffeine decreased the rate of inactivation of adrenaline by O-methylation.4. These findings indicate that methylxanthines potentiate the contractor responses to catecholamines in aortic strips by inhibiting their extraneuronal inactivation.


Inhibition of contact sensitivity in the mouse by topical application of corticosteroids. 1. Topical application of oxazolone to the ears of mice previously sensitized to this hapten resulted in a marked increase in ear weight 24 h later.2. Topical application of hydrocortisone, betamethasone 17-valerate, fluocinolone acetonide or triamcinolone acetonide inhibited this response in a dose dependent manner.3. The free alcohols of the three fluorinated compounds were only weakly active.4. Activity in this model was truly local. Oral administration of hydrocortisone or fluocinolone acetonide at doses comparable to those used topically failed to inhibit the ear swelling.5. Some steroids with poor anti-inflammatory properties had no significant effect.


Structure-activity studies on a 5-hydroxytryptamine receptor of Helix aspersa neurones.


Adrenoceptors in the guinea-pig colon.

Adrenoceptors in the guinea-pig colon. Guinea-pig colon was shown to contain both alpha- and beta-adrenoceptors. Isoprenaline-induced relaxation was mediated by beta-adrenoceptors alone whilst that produced by phenylephrine was mediated by both alpha- and beta- adrenoceptors.


Distribution of bound  3 H-benzilylcholine mustard in subcellular fractions of smooth muscle from guinea-pig ileum.


The amiloride receptor.

The amiloride receptor.


Inhibition of prostaglandin synthesis augments the effects of sympathetic nerve stimulation on the cat spleen.


Rabbit-aorta contracting substance (RCS) may be a prostaglandin precursor.


Effect of sex hormones on the concentrations of plasma kininogen in the female rat.


Inhibition of histidine decarboxylase in rat stomach by aminoguanidine.


Vascular reactivity in 'post-DOCA' hypertension.


Effects of catecholamines on the electrical and mechanical activity of the guinea-pig ureter.


Inhibition of the longitudinal muscle of rabbit duodenum.


Pharmacological observations on the vas deferens of the mouse.


The rat anococcygeus; a new, densely innervated smooth muscle preparation.


Theophylline and adenosine at the neuromuscular junction.


Accumulation of calcium at the motor endplate.


Pharmacological interaction of lorazepam with thiopentone sodium and skeletal neuromuscular blocking drugs.


Action of edrophonium on acetylcholinesterase at the mammalian neuromuscular junction.


Influence of folic acid on noradrenaline stimulation of rat brain synaptosomes.


Supersensitivity of central noradrenaline receptors after reserpine.


Hypnogenic properties of succinic semialdehyde and its fatty acid derivatives.


Influence of ouabain, DNP and DMSO on amino-acid transfer out of the subarachnoid space.


Effect of drugs used in status-epilepticus on the potassium fluxes of cerebrospinal fluid in the conscious dog.


Can drugs replace hypoxic drive in respiratory depression?


Evidence for an active uptake of noradrenaline in the guinea-pig isolated trachea.


Effects of several muscarinic agonists on cardiac performance and the release of noradrenaline from the sympathetic nerves of the rabbit heart.


Demethylation of 3-O-methyldopa.

Demethylation of 3-O-methyldopa.


Comparison of effects exerted by isomers and analogues of ( )-w,3-dehydroemetine on protein synthesis in rat liver.


Vascular changes in tumours after treatment with ICRF 159.


Influence of phenobarbitone on liver regeneration and microsomal N-demethylating activity in partially hepatectomized rats.


Absorption, distribution and excretion of methsuximide in male rats.


Anti-thrombotic activity of a benzo(c)(1,6)naphthyridine.


Differential effect of atropine and hyoscine on human learning capacity.


Relationship of plasma concentrations of levodopa to clinical response in Parkinsonsim.


Plasma concentrations of fenfluramine and its metabolite, norfenfluramine, after single and repeated oral administration.


Pharmacokinetics of inhaled salbutamol in asthmatic patients.


Potentiation of the cardiovascular effects of some catecholamines by a monoamine oxidase inhibitor.


Interactions between catecholamines and tricyclic and monoamine oxidase inhibitor antidepressive agents in man.


Absorption, distribution and elimination of  14 C-amiloride in normal human subjects.


Photometric method for the measurement of cell migration inhibition.


Non-traumatic blood collection from rat tail vessels. Application in Mycoplasma arthritidis.


Implantation of electrodes in the dentine of an upper canine tooth in the dog.


Routine assessment of drug-related induction of liver microsomal enzymes during toxicity trials in animals.


Method for the production and detection of epileptogenic lesions in rat cerebral cortex.


Influence of pH on absorption of thymoxamine through buccal mucosa in man.


Methods to linearize the lower end of a dose-response curve.


Use of a digital computer programme as a guide to the prescribing of kanamycin in patients with renal insufficiency.


Esophageal atresia: a comprehensive study of 210 patients.


Experimental and clinical investigations for the operative treatment of incompetence at the cardia.


A conservative operation for localised reflux and emetogenic strictures of the oesophagus.


An experimental comparison of procedures for the operative correction of gastroesophageal reflux.


Selective proximal vagotomy with and without pyloroplasty in the treatment of duodenal ulcer.


Partial cell vagotomy for duodenal ulcer.


Measurements of concentrations of human serum immunoglobulins.


Omental transposition for the relief of ascites in portal hypertension of bilharzial origin.


Surgical aspects of severe liver trauma.


Leucocyte migration inhibition with Kveim antigen in Crohn's disease.


Delayed cutaneous sensitivity reactions to autologous Burkitt lymphoma protein extract. Results of a prospective two and a half year study.


Clinical and experimental studies on gastric function after several types of vagotomy.


The surgical management of liver injuries.


Factors influencing survival of patients with gastric carcinoma.


Immunological responses in Sjogren's syndrome and rheumatoid arthritis.


Synthesis and secretion of IgE by an established human myeloma cell line.


Sensitive test for antibody type I to intrinsic factors.


Hormones and the immunological capacity. V. Modification of growth hormone-producing cells in the adenohypophysis of neonatally thymectomized germ-free mice: an electron microscopical study.


A comparison of immunosuppressive and anti-inflammatory agents in the rat.


Instrinsic defect of the polymorphonuclear leucocyte resulting in impaired chemotaxis and phagocytosis.


Involutive pathology of the sphincterian system of Oddi.


Chromates as the cause of contact dermatitis in housewives.


Late mutilating bejel in the nomadic Bedouins of Kuwait.


Unusual skin lesions of rheumatoid arthritis.


Retinoic acid in the treatment of lichen planus.


Autoradiography of nucleic acids and proteins combined with histochemical demonstration of enzymes.


Glucose-6-phosphate dehydrogenase (G-6-PD) reaction in eyeballs of ageing rats.


Associative overdominance caused by linked detrimental mutations.


A simple technique for the identification of chain termination suppressor mutants in species of Salmonella.


Improved techniques for duplicating color slides with reversal materials.


The problems and techniques of underwater photography.


Photographic cabinet for recording of thin layer chromatograms.


Instant monochrome 35-MM (2x2 inch) slides.


Fluorescence photomacrography.

Fluorescence photomacrography.


Sterilization of the Mediterranean fruit fly by irradiation: comparative mating effectiveness of treated pupae and adults.


Toxic and teratogenic effects of saturated and unsaturated fatty acids on house fly larvae.


Bioassays on the activation and deactivation of some new insecticides in a mineral soil and absorption of toxic components by rutabagas.


Bioactivity and persistence of some new insecticides in a mineral soil.


Comparative toxicity of four halogenated organophosphorus insecticides to chicks, Japanese quail, and diptera.


Residues of dichlorvos, diazinon, and dimetilan in milk of cows fed PVC-insecticide feed additives.


Degradation of heptachlor epoxide and heptachlor by a mixed culture of soil microorganisms.


Insecticidal effects on soil arthropods.


Residues of compound 4072 in milk and meat of cattle held in barns treated with residual sprays.


The insecticidal action of some sulfonamides.


Cattle grub control in Wyoming with a late-Summer dust-bag application of prolate.


Evaluation of insecticides to control the chigger Neoschongastia americana on turkeys.


Chemosterilization of house flies with anthramycin methyl ether.


In vivo syntheses of phosphatidyl choline and ethanolamine in larvae of the dried-fruit moth.


Effects of moulting hormone and juvenile hormone on insect endocrine gland activity.


Digestive enzymes in the excreta of Aedes aegypti larvae.


Properties of a viral factor toxic to the parasitoid, Apanteles militaris.


Digestion of the avian blood-meal by the mosquito, Aedes aegypti.


Utilization of d-isomers of the dietary indispensable amino acids by Argyrotaenia velutinana larvae.


Physiological effects of carbohydrates on survival, metabolism, and flight potential of female Aedes taeniorphynchus.


Sex pheromone of the grape berry moth: identification by classical and electroantennogram methods, and field tests.


Evidence for visual function of corneal interference filters.


DNA-RNA hybridization studies on ecdysone-induced wing development in the cecropia silkmoth.


Studies on the endocrine control of postemergence ovarian maturation in Manduca sexta.


Neuroendocrine involvement in the delayed pupation of space-deprived Galleria mellonella (Lepidoptera).


Digestion of the human blood-meal by the mosquito, Aedes aegypti.


Mast cells in lymph nodes.

Mast cells in lymph nodes.


Flight performance and metabolism of the moth Spodoptera frugiperda.


A method for increasing the duration of biological signals to facilitate off-line analysis.


Uptake of sodium and potassium by the leech, Hirudo medicinalis.


The influence of secretion on the elimination of drugs in bile.


Detection of foetal acidosis.

Detection of foetal acidosis.


Infusion of secretin into the hepatic arterial circulation of the rat.


Folate metabolism and reproduction.

Folate metabolism and reproduction.


The effects of intracisternal 6-hydroxydopamine on the development of neurogenic hypertension in the rabbit.


The relationship between deep body temperature and performance on psychometric tests.


The specificity of anti-sera to 17-beta-oestradiol.


An increase in p-nitrophenylphosphate hydrolysis by human red cell membranes on lowering ionic strength.


'Blood-brain barrier' concept and the kinetics of 22Na escape from the central nervous system of Periplaneta americana.


Further studies on the mediation of functional hyperaemia in skeletal muscle.


The influence of chronic administration of pentagastrin upon the pancreas of hypophysectomized rats.


The mechanical properties and heat production of chicken latissimus dorsi muscles during tetanic contractions. 1. A study has been made of the mechanical properties and heat production of chicken anterior (ALD) and posterior (PLD) latissimus dorsi muscles during contractions at 20 degrees C.2. There is a difference between these two muscles in the time course of the isometric response. The PLD reaches maximum tetanic tension 10 times faster and relaxes 8 times faster than ALD. The ratio of heat rate to isometric tension (heat rate/tension x length) for PLD is 7-8 times larger than for ALD.3. ALD maintains substantial isometric tension for more than 2 min of stimulation. In PLD tetanic tension begins to fall after only 1 sec.4. The ALD muscle does not show the ;activation' heat seen at the start of contraction with frog and toad muscle but this may be present in PLD.5. There is a range of stimulation frequencies for both muscles over which the fused tetanic tension increases with stimulation frequency.6. The tension-length curve of ALD has a pronounced plateau and is broader than that of PLD.7. The normalized force-velocity relations for the two muscles are similar and may be fitted by Hill's equation with a value of a/P(0) = 0.15-0.16. The maximum velocity of unloaded shortening of PLD is 4-5 times that of ALD.8. Preliminary experiments indicate that the resting heat rate of both muscles is 4 times greater than that of frog muscle at the same temperature. The recovery heat rate of ALD is similar to that of frog.


A platform for measuring the walking forces exerted by the bovine foot.


The in vivo oxygen capacity of haemoglobin in man.


Responsiveness to visual stimuli of single neurones in the pulvinar and lateral posterior nuclei of the cat's thalamus.


The effect of intravenous infusion of antidiuretic hormone on water intake in the rat. 1. In rats the continuous I.V. infusion of ADH at rates up to 6.0 mu. kg(-1) min(-1) decreased drinking caused by water deprivation, by a 10% increase in osmotic pressure, and by caval ligation.2. Infusion of ADH at rates between 0.07 and 6.0 mu. kg(-1) min(-1) never potentiated drinking nor did it significantly affect the 24 hr water intake of rats that were in water balance at the start of the experiment.3. Urine output was slowed by a rate of infusion (13.0 muu. kg(-1) min(-1)) which was approximately 150 times smaller than the lowest rate which was found to decrease water intake.4. Since the rates of infusion of ADH which directly affect water intake and urine flow are so different, it is unlikely that ADH is a general homoeostatic hormone which controls both thirst and urine output in the rat.


Permeability of lung capillaries and alveoli to non-electrolytes in the foetal lamb. 1. Two sets of experiments were performed on intact foetal lambs exteriorized at Caesarean section; in one set radioactively labelled test substances (inulin, sucrose, mannitol, erythritol, urea) were injected I.V. either singly or in pairs and then followed in plasma, lung lymph and alveolar liquid; in the other set labelled test substances (inulin, sucrose, mannitol, erythritol, D-serine, L-serine, D-alpha-alanine, urea, water, thiourea, N-ethylthiourea) were introduced singly, in pairs, or sequentially into alveolar liquid and their concentration followed in alveolar liquid and plasma.2. Inulin was found to cross lung capillary walls but not alveolar walls. Measurements of its concentration following injection into alveolar liquid were used to determine the volume of foetal alveolar liquid (mean = 30 ml./kg) and its rate of formation (mean = 0.036 ml./min.kg). The volume of the lung interstitial space was determined from previous experiments in which [(125)I]PVP had been injected I.V. then measured after 2 hr in lung tissue and lung lymph (mean = 10.4% foetal lung weight after withdrawal of liquid; approximately 20% wet lung tissue weight).3. Transfer constants (min(-1)) for lung capillaries (K(C)) and alveoli (K(0)) were obtained from the experimental results by compartmental analysis. Permeability constants (P(C) and P(O), cm/sec) were derived from them using estimates for capillary and alveolar areas. For lipid insoluble molecules P(C) and P(O) both increased with decreasing molecular radius, the effect being much greater for P(O) than P(C). P(O) was also shown to increase with lipid solubility of the test molecule even though molecular size increased with lipid solubility in the series tested (urea, thiourea, N-ethylthiourea).4. Analysis of the results for the lipid insoluble substances in terms of pore theory gave a value of pore radius for lung capillaries of 150 A and for alveolar walls of 5.5 A. The smallness of the pores in alveolar walls is thought to prevent proteins and other plasma solutes from penetrating alveoli, and to determine the forces which operate in the osmotic flow of water across alveolar walls.


The termination of action of transmitter at the neuromuscular junction as a function of release.


Kininogenase of the guinea-pig's coagulating gland and the release of bradykinin. A kininogenase (CGK) in the coagulating gland of the guinea-pig was partially purified, characterized and separated from a kininase also present in the gland. This kininogenase is not inhibited by the usual kininogenase and protease inhibitors. Our results indicate that it is identical with the esterolytic enzyme in the gland which hydrolyses the synthetic ester TAME. The kinin released by CGK from dog's crude pseudoglobulin was purified more than 1500 times and identified as bradykinin by chromatography on paper, on carboxymethyl-cellulose columns, and on silica gel.


The effect of catecholamines on the spontaneous contractions in bovine mesenteric lymphatics.


The detection of gratings in narrow-band visual noise. 1. The detectability of sinusoidal gratings comprised of either one or many cycles was measured in veiling luminances the spatial frequencies of which were either narrow- or broad-band.2. In narrow-band noise, the single-cycle grating was detected with approximately 0.6 log units less contrast than the many-cycle grating. On the other hand when both broad-band and narrow-band noise were present, there was no measurable difference in the detectability of the two types of grating.3. The results are interpreted as supporting the hypothesis of Campbell & Robson (1968) that spatially varying luminance patterns are processed by mechanisms selectively sensitive to limited ranges of spatial frequencies.


Some characteristics of the CO 2 -sensitive receptors present in the lower respiratory tract of the chicken.


Effect of vasopressin on human blood platelets.


The inhibitory effect of perivascular stimulation on the responses of guinea-pig isolated ileum.


Rouleaux formation and the rate of packing of erythrocytes.


Cardiovascular and respiratory responses to changes in central command durin isometric exercise at constant muscle tension.


Release of an antidiuretic substance by bradykinin in the rat. 1. Water-loaded rats under ethanol anaesthesia were used to determine whether bradykinin releases ADH and whether the release is a reflex response to hypotension or the result of direct stimulation of the central nervous system by the peptide.2. Intravenous injections of bradykinin caused hypotension followed by prolonged antidiuresis; both responses were dose dependent.3. The antidiuresis caused by bradykinin could not be accounted for by hypotension alone. Haemorrhage and intravenous injections of isoprenaline, which caused falls in arterial blood pressure similar to those caused by bradykinin, produced only short-lasting antidiuretic responses.4. Intracarotid injections of bradykinin caused antidiuresis with little or no preceding hypotension. No antidiuresis was produced by intracarotid injections of isoprenaline.5. After intravenous injection of bradykinin, small amounts of ADH were identified in whole blood and, on a few occasions only, in concentrated urine. However, ADH was not detected in blood extracts.6. After intravenous injection of nicotine in doses which caused antidiureses similar to that caused by bradykinin, ADH was regularly detected in large amounts in blood extracts and in unconcentrated urine.7. Bradykinin did not cause prolonged antidiureses when injected into a rat with congenital diabetes insipidus.8. ADH release was not increased when isolated rat neural lobes were incubated with bradykinin although release was elevated by an increase in the K(+) concentration of the incubation medium.9. It is suggested that bradykinin causes release of an antidiuretic substance by a direct action on the central nervous system. The possibility that this substance is ADH is discussed.


Long-term measurements of the heart rate in middle-aged men.


A study of the inferior mesenteric and pelvic ganglia of guinea-pigs with intracellular electrodes. 1. Ganglion cells in the inferior mesenteric ganglion (IMG) and the pelvic plexus of the guinea-pig were studied using intracellular micro-electrodes.2. Ganglion cells had resting membrane potentials of 55-65 mV. Threshold for initiation of an action potential ranged from 10 to 20 mV depolarization. Action potentials often exceeded 100 mV in amplitude and were followed by an after-hyperpolarization of up to 20 mV.3. Synaptic responses were recorded from cells in the IMG in response to stimulation of the right and left hypogastric nerves, ascending mesenteric, inferior splanchnic and colonic nerves. It has been established that more than forty preganglionic fibres converge on any one cell. Preganglionic fibres to the IMG were also observed in the pelvic nerves.4. In contrast to the IMG, ganglion cells in the pelvic plexus received up to ten preganglionic fibres.5. Ganglion cells responded to supramaximal preganglionic stimulation with up to four action potentials.6. In the IMG, action potentials in response to synaptic action were followed by a prolonged period of hyperpolarization (after-hyperpolarization) and a later phase of prolonged depolarization (after-depolarization). The time course of these after potentials depended on the pattern of firing of action potentials during the period of stimulation. In the presence of dihydro-beta-erythroidine, or if synaptic action was insufficient to evoke action potentials, only the after-depolarization was observed.7. Other cells were impaled whose properties differed from those described above. In one group of cells the resting membrane potentials were higher (up to 85 mV), input resistances lower and the threshold for initiation of an action potential was higher. The other group were inexcitable, had high resting membrane potentials (up to 85 mV), low input resistances and underwent a slow depolarization in response to repetitive stimulation of preganglionic fibres.8. This study indicates that marked convergence of presynaptic fibres occurs on to ganglion cells of the IMG. The ganglion cells in the pelvic plexus receive a relatively small number of fibres, many of which exert intense synaptic activity ensuring a direct connexion to the central nervous system.


Excitatory input from the distal colon to the inferior mesenteric ganglion in the guinea-pig. 1. Intracellular recordings were obtained from ganglion cells in the guinea-pig inferior mesenteric ganglion (IMG) with a segment of the distal colon attached to the lumbar colonic nerves.2. Continuous electrical activity consisting of excitatory synaptic potentials and action potentials was recorded from ganglion cells in all regions of the IMG.3. The ;spontaneous' synaptic potentials were indistinguishable from those elicited by submaximal stimulation of any of the nerve trunks connected to the IMG.4. The excitatory activity was irreversibly abolished when the lumbar colonic nerves were cut and reversibly abolished when tetrodotoxin (5 x 10(-7) g/ml.) was added to the colon side of a two-compartment organ bath.5. Addition of dihydro-beta-erythroidine (5 x 10(-6) g/ml.) to the ganglion side of the bath abolished the synaptic activity of colonic origin and the synaptic responses to stimulation of any of the nerve trunks connected to the IMG.6. Addition of dihydro-beta-erythroidine (1 x 10(-5) g/ml.) to the colon side of the bath markedly depressed the synaptic input of colonic origin but had no effect on synaptic responses produced by preganglionic nerve stimulation.7. Distension of the colonic segment and the application of 5-HT (1 x 10(-5) g/ml.) to the mucosal surface of the colon increased the frequency of synaptic input.8. The synaptic input from the colon was transiently blocked following repetitive stimulation of any of the nerve trunks connected to the IMG. The discharge of miniature synaptic potentials was unaffected.9. Addition of noradrenaline (1 x 10(-7) to 1 x 10(-6) g/ml.) to the colon side of the bath reduced, and in some cases completely abolished, the synaptic input to the IMG. Phentolamine (1 x 10(-6) g/ml.), when added to the colon side of the bath, blocked the effect of noradrenaline and the transient inhibition following repetitive nerve stimulation.10. Addition of noradrenaline (1 x 10(-4) g/ml.) to the ganglion side of the bath reduced but never abolished the amplitude of the synaptic potentials of colonic origin.11. It was concluded that in the guinea-pig, the IMG is involved in a peripheral reflex whose afferent limit of this reflex consists of the axons of cholinergic neurones within the wall of the colon. Many of these neurones are driven either directly or indirectly by cholinergic synapses. The efferent noradrenergic neurones of the IMG function as a group of inhibitory neurones which depress the activity of the excitatory neurones of the colon which are driving them.


The secretory nerves of the parotid gland of the dog. 1. Secretory nerve fibres to the parotid gland of the dog have been found in the adventitia of the internal maxillary artery. In dogs under chloralose anaesthesia the course of these fibres from the mandibular nerve to the gland was determined by electrical stimulation at different sites. Secretion induced by these nerves was abolished by atropine but not by hexamethonium.2. In dogs in light barbiturate anaesthesia parotid secretion was evoked reflexly by pouring citric acid on the tongues. After division of the auriculo-temporal nerve and the nerves on the artery this secretion disappeared in about half the experiments and was greatly reduced in the others.3. The maximal secretory rate which could be obtained reflexly from the normally innervated parotid gland was first estimated in light barbiturate anaesthesia. Chloralose was then given and the auriculo-temporal nerve and the nerves on the artery were electrically stimulated together; in about half the experiments this could produce secretion at a rate as high as that obtained reflexly.4. By separate and simultaneous stimulation of different sets of nerve fibres convergence of the secretory fibres to the glandular cells could be demonstrated.5. It is concluded that a large number of post-ganglionic, cholinergic secretory fibres to the canine parotid gland run on the internal maxillary artery. In some dogs a small number of fibres seem to have escaped detection. Possibly they may reach the gland by way of the facial nerve.


Afferent discharges from arterial mechanoreceptors in the kidney of the rabbit. 1. Afferent discharges were observed in dissected filaments or single nerve fibres of renal nerves in the rabbit.2. Increasing the arterial perfusion pressure in excised kidney preparations caused an increase in afferent discharge rate. Increasing the venous pressure was without effect. Receptor discharge patterns were compatible with those of the slowly adapting type.3. In vivo, increasing the systemic blood pressure by means of the sinus reflex caused an increase in afferent discharge rate. Afferent discharge patterns showed no synchronicity with heart beat, irrespective of the blood pressure levels.4. It is suggested that mechanoreceptors are present around the arterial walls in the kidney and that they send information about blood pressure levels in the renal artery to the central nervous system.


Sodium transport by perfused giant axons of Loligo. The sodium efflux from perfused squid giant axons has been studied using radioactive sodium, and the sufficient conditions for the maintenance of a potassium- and ouabain-sensitive sodium efflux have been established. The following were found.1. Axons extruded and then perfused with their own axoplasm had a sodium efflux which was sensitive to cyanide, potassium and ouabain and was thus similar to the efflux from intact axons.2. A method for replacing natural axoplasm into fibres previously perfused with artificial axoplasm was developed and used to establish an artificial perfusate that was not irreversibly toxic.3. Short perfusion (5 min) with a variety of artificial perfusates was then found to give fibres which had potassium- and ouabain-sensitive sodium effluxes when ATP was present in the perfusate.4. In the absence of ATP the sodium efflux was small and relatively insensitive to both external potassium and to ouabain.5. With ADP in the perfusate, fibres gave a sodium efflux which was ouabain-sensitive but was little affected by the removal of external potassium from the sodium-rich sea water bathing the fibres.6. The perfused fibres differed from intact fibres in having large ouabain-insensitive sodium effluxes.7. After very long perfusions (40-90 min), with the simple media containing ATP, the rate constant for sodium efflux from the fibres tended to be large and was relatively insensitive to potassium or to ouabain.8. Fibres refilled with natural axoplasm after long perfusion showed increased sensitivity to external potassium; refilled with dispersed axoplasm the sodium efflux tended to become very large.9. After very long perfusions with artificial axoplasms containing ATP, a potassium- and ouabain-sensitive sodium efflux was found to persist provided that dextran was present and the total osmotic pressure and the hydrostatic pressure of the perfusate were controlled. Under these conditions the sodium efflux resembled that from briefly perfused fibres. The necessary and sufficient conditions for the maintenance of sodium transport by perfused giant axons are discussed.


A simple method for complete processing of data from liquid scintillation counters.


The effects of prolonged neostigmine treatment.


(Ureyl- 14 C) tolbutamide and (2- 14 C) pentobarbitone as substrates for the hepatic microsomal drug-metabolizing enzyme system in vitro.


The fine structure of the cutaneous poison glands in Xenopus laevis.


The effects of conjugation of cholic acid on its transport from blood to bile in the dog.


Oestradiol-binding capacity in neural and non-neural target tissues of neonatally androgenized female rats.


Changes in non-specific esterase and acid phosphatase activities, isozymic patterns and cellular distribution due to differences in the human sera component of the medium in HeLa cells.


Fine structure, distribution and function of the rat thymic reticular cells in the perinatal life.


Structure of the medullo-spinal liquor-contacting neuronal system.


Phylogenetic, ontogenetic and experimental changes in the structure of the spine.


A morphometrical study of the aeration of the pulmonary parenchyma in neonatal hyaline membrane disease.


Microradiographic evaluation of bone and ultrastructure of C-cells and parathyroid glands of cows receiving parathyroid extract.


Regeneration of liver parenchyma under normal and pathological conditions.


A study of mitochondrial complementation in wheat.


Allosteric properties of wheat-germ aspartate transcarbamoylase.


Acid phosphatase and ribonuclease in the cotyledons of germinating peas.


The sites of synthesis of chloroplast proteins.


A T4-bacteriophage reversion test for the naturally occurring mutagen present in bracken fern (Pteridium aquilinum).


Turnover of deoxyribonucleic acid in seedlings of Pisum sativum.


Isolation of an active principle from the bracken fern that is mutagenic, carcinogenic and lethal to mice on intraperitoneal injection.


Alkaline reductive cleavage of 4-N-(2-acetamido-2-deoxy- -D-glucopyranosyl)-L-asparagine.


The biliary excretion of amphetamine and methamphetamine in the rat.


The metabolism or (35S)homosulphanilamide.


Protection by zinc ions against the toxicity of cadmium ions.


The control of seed dormancy.

The control of seed dormancy.


Desulphation of L-fucose monosulphates by an enzyme from Patella vulgata.


Localization of monomethyl- and dimethyl-tocols on cytoplasmic particles in plant tissues.


The uptake and utilization of beta-tocopherol by higher plants and algae.


Inhibition of photosynthetic electron transport by 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) at a site before photosystem. 2.


A heparan sulphate sulphotransferase of ox lung.


Lipid metabolism by rat lung in vitro. Effect of starvation and re-feeding on utilization of (U- 14 C)glucose by lung slices. 1. The incorporation of [U-(14)C]glucose into several lipid components of lung and liver slices, and the activities of glucose 6-phosphate dehydrogenase (EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 1.1.1.44), ;malic' enzyme (EC 1.1.1.40) and NADP-isocitrate dehydrogenase (EC 1.1.1.42) of the cell cytosol were examined in normal, starved and re-fed rats. 2. Lipogenesis and the activities of these enzymes in liver were decreased markedly in rats starved for 72h. Re-feeding starved rats on a fat-free diet for 72h resulted in the well documented hyperlipogenic response in liver, particularly in its ability to convert glucose into neutral lipid, and increased activities of glucose 6-phosphate dehydrogenase, ;malic' enzyme and 6-phosphogluconate dehydrogenase to values approx. 700, 470 and 250% of controls respectively. 3. Approx. 70% of the total label in lung lipids was present in the phospholipid fraction. Hydrolysis of lung phospholipids revealed that lipogenesis from glucose was considerable, with approx. 40% of the total phospholipid radioactivity present in the fatty acid fraction. 4. Incorporation of glucose into total lung lipids was decreased by approx. 40% in lung slices of starved rats and was returned to control values on re-feeding. Although phospholipid synthesis from glucose was decreased in lung slices of starved rats, the decrease proportionally was greater for the fatty acid fraction (approx. 50%) as compared with the glycerol fraction (approx. 25%). 5. The activities of lung glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and NADP-isocitrate dehydrogenase were not affected by the dietary alterations. ;Malic' enzyme activity was not detected in lung cytosol preparations. 6. The results are discussed in relation to the surface-active lining layer (surfactant) of the lung.


Investigation on the effects of cigarette smoke on rabbit alveolar macrophages.


Further observations on the alpha-galactosidase activity of Vicia faba seeds.


Cellular injury and carcinogenesis. The effect of a protein-free high-carbohydrate diet on the metabolism of dimethylnitrosamine in the rat. 1. Rats fed on a protein-free high-carbohydrate diet for 7 days metabolized dimethylnitrosamine at only 55% the rate of rats fed on a commercial diet. 2. Dimethylnitrosamine was metabolized by liver slices from rats fed on the protein-free diet at less than half the rate attained by slices from rats fed on a commercial diet. But kidney slices from these rats metabolized dimethylnitrosamine at the same rate as kidney slices from rats on a commercial diet. 3. Methylation by dimethylnitrosamine (70mg/kg body wt.) of N-7 of guanine of the liver RNA and DNA of rats fed on a protein-free diet was only slightly higher than in rats fed on a normal diet given 27mg/kg body wt. In contrast, the methylation by dimethylnitrosamine of guanine in kidney nucleic acids of these rats was three times that in the rats fed on a normal diet. 4. In rats fed on a protein-free diet the incidence of kidney tumours produced by a single dose of dimethylnitrosamine is increased.


Phenolic metabolism in potato tuber tissue after infection with phytophthora infestans.


Competitive labelling, a method for determining the reactivity of individual groups in proteins. The amino groups of porcine elastase. 1. A method is described for determining the ionization constants and reactivities of individual amino groups in proteins. The principle is that in the presence of a trace amount of radioactive label, the various reactive groups in a protein molecule will compete for the label and the amount incorporated into any one group will be determined by its nucleophilicity, pK and micro-environment. The relative amounts of label incorporated into various groups will be proportional to their second-order rate constants and by comparing these rate constants with those expected on the basis of a linear free-energy relationship obtained with a series of standard compounds, the micro-environment can be defined for a particular amino group. 2. The method consists of treating a protein and an internal standard with a limiting amount of radioactive reagent and then with an excess of unlabelled reagent to yield a chemically homogeneous but heterogeneously labelled compound. After appropriate enzymic digestion peptides containing each labelled group are isolated and their rates of reaction, relative to the internal standard, are determined from their specific radioactivities. The entire procedure is repeated at several pH values. 3. When the method was applied to the amino groups of porcine elastase by using tritiated acetic anhydride as the labelling reagent, the N-terminus was found to have pK(a) 9.7 and a much lower than normal reactivity. Lysine-87 and lysine-224 were found to have pK(a) 10.3 and normal reactivities. At pH values greater than 10.5 there are discontinuities in all the titration curves, indicating that the entire molecule is undergoing a structural reorganization.


The possible human hazard of the naturally occurring bracken carcinogen.


The effects of gamma-irradiation on the amino acid content of proteins.


Gibberellins and carbohydrate mobilization in germinating barley grains.


Allotypically related sequences in the Fd fragment of rabbit immunoglobulin heavy chains. The sequence has been completed of the N-terminal 94 residues of the variable section of the Fd fragment of heavy chains from rabbit immunoglobulin G (IgG) of allotype As1. Most of the sequence of the same section from IgG of allotype Aa3 is also reported. These results, in conjunction with a substantial sequence of the variable region of allotype Aa2 reported elsewhere (Fleischman, 1971), show the presence of 16 positions (including six consecutive positions) in which the residue present correlates with the allotype. No allotype-related sequence variation has been found in the constant section of the Fd fragment. This evidence supports the view that two genes code for the heavy chain and it can be used as evidence in favour of somatic mutation as the origin of the variability in the sequence of the N-terminal section. The evolutionary origin of the ;a' locus allotypes of rabbit immunoglobulins remains obscure.


Thiamin balance in the sheep.

Thiamin balance in the sheep.


Growth of Pseudomonas reptilivora on glycine.


Studies on the primary structure of chicken erythrocyte histone fraction V. Histone fraction V was prepared by selective acid extraction from chicken erythrocyte nuclei. Amino acid sequence studies on the tryptic and thermolytic peptides are reported. Only a limited amount of overlapping sequence data was obtained.


Growth of Pseudomonas reptilivora on N-methylglycines.


Plastocyanin from Cucurbita pepo L.

Plastocyanin from Cucurbita pepo L.


Protein synthesis by brain-cortes mitochondria. Characterization of a 55S mitochondrial ribosome as the functional unit in protein synthesis by cortex mitochondria and its distinction from a contaminant cytoplasmic protein-synthesizing system. Homogenates of rat brain cortex were fractionated by conventional methods of velocity sedimentation and separated into a microsomal and a washed mitochondrial fraction. By electron microscopy the mitochondrial fraction was shown to be rich in synaptosomes. The mitochondria-synaptosome fraction synthesized protein in vitro by a route that was partially inhibited by cycloheximide and partly by chloramphenicol. The relative effectiveness of the two inhibitors varied greatly with the medium used. In the mitochondria-synaptosome fraction active 80S cytoplasmic ribosomes and active 55S mitochondrial ribosomes were detected; these were also seen in the electron microscope. Mild osmotic shock of the mitochondria-synaptosome fraction followed by velocity sedimentation in sucrose-EDTA allowed isolation of a mitochondrial fraction free of synaptosomes. Protein synthesis in this fraction was entirely inhibited by chloramphenicol, but was completely resistant to cycloheximide both in a medium promoting oxidative phosphorylation and in ATP-generating medium. Ouabain had no inhibitory effect on protein synthesis in a purified mitochondrial preparation. It is concluded that brain-cortex mitochondria synthesize protein entirely on 55S mitochondrial ribosomes.


The bursting response of isolated avena coleoptile protoplasts to indol-3-ylacetic acid.


-Crystallin. The isolation and characterization of distinct macromolecular fractions. alpha-Crystallin was isolated from calf lens periphery by chromatography on DEAE-cellulose and gel filtration. Three distinct populations of macromolecules have been isolated with molecular weights in the ranges approx. 6x10(5)-9x10(5), 0.9x10(6)-4x10(6) and greater than 10x10(6). The concentration of macromolecules at the molecular-weight limits of a population are very low. The members of the different populations do not appear to be in equilibrium with each other. Further, in those molecular-weight fractions investigated, no equilibrium between members of the same population was observed. The population of lowest molecular weight comprises 65-75% of the total material. The amino acid and subunit composition of the different-sized fractions appear very similar, if not identical. The only chemical difference observed between the fractions is the presence of significant amounts of sugar in the higher-molecular-weight fractions. Subunit molecular weights of approx. 19.5x10(3) and 22.5x10(3) were observed for all alpha-crystallin fractions.


-Crystallin. Fractionation of subunits and sequence studies on an isolated polypeptide. alpha-Crystallin was carboxymethylated with radioactive iodoacetic acid in the presence of 7.6m-urea and then separated into six major fractions by chromatography on DEAE-cellulose in 7m-urea. Based on the amino acid compositions, specific radioactivities and sodium dodecyl sulphate-gel electrophoresis of the fractions, it was concluded that alpha-crystallin contains at least four different subunits: DU1A and DU1B, containing no cysteine; a third component represented by DU2B and DU3 containing one cysteine one cysteine residue per subunit; and DU4, which probably contains two residues of cysteine per subunit. Subunit DU1A was shown to be of sufficient purity for sequence studies. Cyanogen bromide cleavage yielded two peptides, CB-1 and CB-2, in approximately equal amounts as expected. The sum of the molecular weights and amino acid compositions of the peptides were both in excellent agreement with the results obtained for subunit DU1A. The amino acid sequence of the first sixteen residues of peptide CB-1 is: Ser-Leu-Thr-Lys-Asp-Phe-Asp-Glu-Val-Asn-Ile-Asp-Val-Ser-His-Phe-. The sequence of the first seventeen residues of peptide CB-2 is: Asp-Ile-Ala-Ile-Ser-His-Pro-Trp-Ile-Arg-Pro-Ser-Phe-Phe-Glu-Phe-His-. The N-terminal sequence of subunit DU1A was shown to be N-acetylmethionine followed by peptide CB-2.


The nucleotide sequences of some large ribonuclease T 1  products from bacteriophage R17 ribonucleic acid. A method of ;fingerprinting' high-molecular-weight (32)P-labelled RNA species, using a two-dimensional thin-layer-chromatographic separation of ribonuclease T(1) digestion products, has been applied to RNA from the Escherichia coli bacteriophage R17. The ;fingerprinting' technique, besides giving a unique pattern that can be used as a characterization of the RNA, has made it possible to isolate a number of the larger oligonucleotides and to determine their nucleotide sequences.


The isolation and properties of the protein calliphorin. 1. A procedure for the isolation of the protein calliphorin from larvae and pupae of the blowfly Calliphora erythrocephala is described. 2. The calliphorin preparation shows a single component in the ultracentrifuge at pH6.3 and gives a single band when stained for protein after agar-gel or starch-gel electrophoresis at pH6.3 or 8.6. Immunoelectrophoresis yields only one arc, associated with the stained spot, to a rabbit antiserum known to react with 13 other soluble components of Calliphora pupae. 3. Calliphorin has s(0) (20,w) 19.4S, D(0) (20,w) 3.25x10(-7)cm(2).s(-1) and f/f(0) 1.22, indicating a molecular weight of 528000 and a compact symmetrical structure. The molecular weight determined by the meniscus-depletion sedimentation-equilibrium method is 529000. 4. In 6.2m-guanidine hydrochloride calliphorin dissociates into six components each with a molecular weight of about 87000. Calliphorin reversibly dissociates into components with sedimentation coefficients of about 7S as the pH is raised progressively above pH6.5. 5. Calliphorin has an unusually high tyrosine and phenylalanine content (442 and 400mol/mol of protein respectively), a relatively high methionine content (162mol/mol of protein) and very little cystine or cysteine (18mol/mol of protein). The E(280)/E(250) ratio is 3.2. The pure protein contains 0.4-0.5% carbohydrate. 6. When examined in the electron microscope by the negative staining technique the protein is seen to consist of particles which are right prisms, being 105A wide and 65A high, rectangular in side view and curvilinear equilateral triangles in surface view.


Sterol and triterpene synthesis in the developing and germinating pea seed. Developing and germinating pea seeds were compared with respect to their capacity to incorporate mevalonate into sterols and triterpenes. The capacity for sterol synthesis is greatest in the least mature fruits and decreases during their development. Label is shown, by gas-liquid chromatography and counting the radioactivity of trapped fractions, to be associated with campesterol, beta-sitosterol and isofucosterol. During early stages of germination sterol synthesis is insignificant. The triterpene fraction becomes heavily labelled during both development and germination. The label is associated almost exclusively with beta-amyrin during germination but with cycloartenol and 24-methylenecycloartanol during development. It is only in the terminal stages of maturation that beta-amyrin becomes significantly labelled. At the same time an unidentified radioactive polar compound appears. The possible significance of the appearance of this polar compound and the regulation of the synthesis of these higher terpenoids is discussed.


Biochemical studies of the peroxidase-mediated oxidation of tyrosine to melanin: demonstration of the hydroxylation of tyrosine by plant and human peroxidases.


Amino acid regulation of synthesis of ribonucleic acid and protein in the liver of rats. Weanling (23-day-old) rats were fed on either a low-protein diet (6% casein) or a diet containing an adequate amount of protein (18% casein) for 28 days. Hepatic cells from animals fed on the deficient diet were characterized by markedly lower concentrations of protein and RNA in all cellular fractions as compared with cells from control rats. The bound rRNA fraction was decreased to the greatest degree, whereas the free ribosomal concentrations were only slightly less than in control animals. A good correlation was observed between the rate of hepatic protein synthesis in vivo and the cellular protein content of the liver. Rates of protein synthesis both in vivo and in vitro were directly correlated with the hepatic concentration of individual free amino acids that are essential for protein synthesis. The decreased protein-synthetic ability of the ribosomes from the liver of protein-deprived rats was related to a decrease in the number of active ribosomes and heavy polyribosomes. The lower ribosomal content of the hepatocytes was correlated with the decreased concentration of essential free amino acids. In the protein-deprived rats, the rate of accumulation of newly synthesized cytoplasmic rRNA was markedly decreased compared with control animals. From these results it was concluded that amino acids regulate protein synthesis (1) by affecting the number of ribosomes that actively synthesize protein and (2) by inhibiting the rate of synthesis of new ribosomes. Both of these processes may involve the synthesis of proteins with a rapid rate of turnover.


Triglyceride mobilization in the germinating wheat grain.


Binding of carcinogenic aromatic amine to rat liver nuclear acidic proteins in vivo.


Preformed messenger of quiescent wheat embryos.


An examination of the evidence for stable messenger ribonucleic acid in seed.


Protein synthesis and viability in rye.

Protein synthesis and viability in rye.


Formation and utilization of ketone bodies during development of the male rat.


The metabolism of 4-chloro-2-methylphenoxyacetic acid in plants.


The subcellular distribution of tocopherols in the green leaves of Pisum sativum.


Induced myogenic activity in the neurogenic heart of Limulus polyphemus.


Corticosterone phases a circadian water-drive response to prolactin in the spotted newt, Notopthalmus viridescens.


The torsional characteristics of human skin in vivo.


Heterocyclic systems bearing phosphorus substituents. synthesis and chemistry.


X chromosome inactivation mosaicism in the mouse.


Clonal parameters of tergite development in Drosophila.


DNA synthesis inhibition and cell death associated with hydroxyurea teratogenesis in rat embryos.


Growth of the preimplantation embryo of the northern fur seal and its correlation with changes in uterine protein.


Are the genes for hemoglobin reiterated?


Advances in nucleic acid hybridization.

Advances in nucleic acid hybridization.


A new model of gene expression in eucaryotic cells.


Some epidemiologic factors associated with intramural football injuries on a rural college campus.


Life change and infectious monoucleosis.


Health care utilization in university student families.


Problems of ecology on the college campus: the socio-cultural environment.


Physiological evidence for variations in intellectual circadian periodicity.


Psychological services at a small liberal arts college.


A study of the relationship between physical activity participation and health service visits of Washington State University students.


Acute appendicitis during a campus outbreak of gastroenteritis.


Concept to action: Student Dental Health Programs.


An experimental dental care program for a college community.


Patterns of drug usage among university students. IV. Use of marihuana, amphetamines, opium, and LSD by undergraduates.


Monoethylenic fatty acids of a partially hydrogenated herring oil.


Crambe seed processing: decomposition of glucosinolates (thioglucosides) with chemical additives.


Studies on the inactivation and regeneration of streptothricin.


Studies on the metabolism of cephalosporins. II. Biliary excretion and metabolism of 14 C-cephachlomezine and its parent compound.


Tetranactin, a new miticidal antibiotic. V. Quantitative determination macrotetrolide antibiotics.


Studies on tuberactinomycin. II. Isolation and properties of tuberactinomycin-N, a new tuberactinomycin group antibiotic.


The identity of citromycin with LL-AC541, E-749-C, and BY-81.


A folic acid linked system in bacterial cell wall synthesis?


The structure of the antifungal antibiotic versicolin.


Antibacterial activity of some acetyl derivatives of kujimycin A.


Antitumor activity of cytochalasin D.

Antitumor activity of cytochalasin D.


Total synthesis of antimycin A 3.

Total synthesis of antimycin A 3.


Leucylnegamycin, an antibiotic from negamyhcin-producing Streptomyces.


Intracellular potentials in bull spermatozoa.


On the direct and crossed components of reflex responses to unilateral stimulation of the carotid body chemoreceptors in the dog. 1. In dogs under chloralose-urethane anaesthesia the chemoreceptors of the two carotid bodies were separately stimulated.2. The distribution of three primary reflex responses to carotid body stimulation was studied: parasympathetic bradycardia, sympathetic vasoconstriction, and increase in somatic phrenic nerve activity.3. The reflex bradycardia evoked by either carotid body was mediated by both vagus nerves, but when either vagus was blocked a greater response could be obtained from the contralateral than from the ipsilateral carotid body.4. The reflex vasoconstriction evoked by either carotid body was seen in both hind limbs, with no predominance in either limb.5. The reflex increase in phrenic nerve activity evoked by either carotid body was seen in both phrenic nerves, with no predominance in either nerve.


Na and Ca components of action potential in amphioxus muscle cells. 1. The ionic mechanism of the action potential produced in lamella-like muscle cells of amphioxus, Branchiostoma californiense, was investigated with intracellular recording and polarization techniques.2. The resting potential and action potential overshoot in normal saline are -53+/-5 mV (S.D.) and +29+/-10 mV (S.D.) respectively.3. The action potential is eliminated by tetrodotoxin (3 muM) and by replacing NaCl in the saline with Tris-chloride but maintained by replacing Na with Li.4. After elimination of the normal action potential by tetrodotoxin or replacing Na with Tris, the addition of procaine (7.3 mM) to the external saline makes the membrane capable of producing a regenerative potential change.5. The peak potential of the regenerative response depends on external Ca concentration in a manner predicted by the Nernst equation with Ca concentrations close to normal.6. The Ca dependent response is reversibly suppressed by Co or La ions.7. Similar regenerative responses are obtained when Ca is substituted with Sr or Ba.8. It is concluded that two independent mechanisms of ionic permeability increase occur in the membrane of amphioxus muscle cell, one to Na and the other to Ca.


Excitation-contraction coupling in amphioxus muscle cells. 1. Excitation-contraction coupling was studied in myotomal muscles of amphioxus, Branchiostoma californiense.2. The action potential of a muscle cell produces a twitch with a rise time of 30-40 msec at 11 degrees C and its Q(10) is about 2.2.3. The twitch increases in amplitude with increasing external Ca concentration and is abolished in Ca-free saline (1 mM-EGTA and 55.7 mM-MgCl(2)); the twitch amplitude is suppressed by Co or La ions.4. Caffeine at concentrations above 1 mM in the external saline causes a prolongation of the action potential and a contracture which lasts several minutes.5. After exposure to caffeine the responsiveness of the muscle to subsequent applications of caffeine recovers in normal saline in 20-30 minutes but not in Ca-free saline.6. The amplitude of the caffeine contracture is independent of the external Ca concentration and is unaltered after the twitch is eliminated in Ca-free saline.7. After exposure to caffeine a full-sized twitch can be obtained before the responsiveness to caffeine shows any significant recovery.8. It is concluded that the twitch is produced by the Ca influx resulting from the increased permeability of the muscle cell membrane to Ca during the action potential and that the Ca mobilized by caffeine is not necessary to the initiation of the twitch.9. Electronmicroscopy shows the existence of sarcoplasmic reticulum.


Responses of vibration-sensitive receptors in the interosseous region of the duck's hind limb. 1. Responses of receptors with fibres in the interosseous nerve of the duck's leg have been studied by recording unit discharges in filaments dissected from the sciatic nerve.2. Seventy-two of the ninety-four units examined served highly phasic, vibration-sensitive mechanoreceptors in the interosseous region interpreted as being Herbst corpuscles. Receptor types for most of the other units could not be determined, but some were slowly adapting mechanoreceptors.3. Rheobase threshold values for the most sensitive vibration-receptors were similar to those of mammalian Pacinian corpuscles.4. Threshold-frequency relationships for the vibration receptors showed a wider range of low frequency cut-off values, and a greater capacity to signal high frequencies, than is the case with Pacinian corpuscles.5. Fibres of the vibration-receptors had calculated diameters ranging from 5 to 10 mum and account for the bulk of the larger fibres in the interosseous nerve.6. It is suggested that Herbst corpuscles in the legs of birds might act as a warning device by detecting vibratory disturbances of the ground or other supporting surface.


Myoepithelial cell activation in the submaxillary salivary gland. 1. Pressure in the duct of the submaxillary gland of the cat was recorded under chloralose anaesthesia. Single pulses applied to the parasympathetic nerve caused a pressure rise.2. Salivation was just detectable in about half of the animals when an open tube was connected to the duct and single pulses applied to the nerve. Applying a back pressure of 1-4 mm Hg renders the duct less distensible and under these circumstances secretion of 1-3 mul. fluid was always seen. An alternative method of detecting salivation based on the pressure record itself also showed that salivation occurred in response to the stimulus and that salivation was responsible for the pressure rise observed.3. Single pulses applied to the cervical sympathetic trunk produced a small fall in pressure. In a short series of pulses this was followed by a slow rise which then slowly decayed. It was tentatively concluded that the initial fall was secondary to the accompanying vasoconstriction but the subsequent effects were due to myoepithelial cell contraction.4. Close arterial injection of bradykinin caused a pressure rise which could best be explained by myoepithelial cell contraction.5. It is concluded that the myoepithelial cells of the submaxillary gland of the cat do not receive a parasympathetic motor innervation but it is probable that they are innervated by the sympathetic nervous system and contract in the presence of bradykinin.


Joint scan with radiotechnetium.

Joint scan with radiotechnetium.


Lactose intolerance in chronic diarrhea among Indonesian children.


Biological availability to chicks of manganese from different inorganic sources.


The effect of a food supplementation programme on the nutritional status of pre-school children.


Investigations on the developing chicken bursa of fabricius.


Semem production in turkeys.

Semem production in turkeys.


The activity of six enzymes of chicken seminal plasma and sperm. 2. The relationship between enzyme activity and fertility of fresh and stored semen.


Standardized management of diarrhoea in young children.


The activity of six enzymes of chicken seminal plasma and sperm. 1. Effect of in vitro storage and full sib families on enzyme activity and fertility.


Small intestine biopsy in protein calorie malnutrition and celiac children.


The effect of nutritional status on some aspects of intelligence.


Selenium content of feedstuffs and effects of dietary selenium levels upon tissue selenium in chicks and poults.


Detection of lysozyme in developing avian embryos.


Tissue clearance of Rofenaid in chickens and turkeys.


Influence of some hypocholesterolemic compounds on the serum protein patterns and serum cholesterol of normal and diethylstilbestrol-treated cockerels.


Effects of essential fatty acid deficiency on coccidiosis in the  domestic fowl.


Differences in erythrocyte measurements of intersex and normal adult chickens.


A study of ochratoxin toxicity in hens.

A study of ochratoxin toxicity in hens.


The effect of environmental temperature on blood volume.


Fluothane gas anesthesia for newly-hatched chicks.


A note on reported levels of estrone in laying hens' blood.


A natural and extremely severe occurrence of aflatoxicosis in laying hens.


Response of the developing embryo to light.


Biochemical research in psychiatry.

Biochemical research in psychiatry.


1. A logical analysis of the medico-psychological concept of disease.


Salivary secretion and depressive illness: a physiological and psychometric study.


Present status of the concept of drug dependence.


Conceptual distance indices as measures of alienation in obsessional neurosis.


Drinking problems among recidivist prisoners.


Danger periods for suicide in patients under treatment.


Severely retarded children in a London area: prevalence and provision of services.


'Secondary' affective disorder: a study of 95 cases.


Medical Research Council Clinical Psychiatry Unit.


Effect of testosterone on erythropoietic unresponsiveness to hypoxia of BALB-ep mice.


Effect of pitressin tannate on the diabetes insipidus induced by aminopterin in the rat.


Effect of plethora on the erythropoietic response of the bone marrow to exogenous erythropoietin in the mouse.


Androgen-protein interactions in rodent submaxillary glands.


Anti-hemostatic action of heparin: relationship between topical and intravenous application.


Studies on the effect of growth hormone upon the rate of disappearance of insulin from blood in the dog.


Afferent structures involved in heart response to diving reflex.


Influence of age and training on the diving reflex response.


A simple bioassay system for erythropoietin.


The androgen dependency of the process of sperm maturation in the guinea pig.


A technique for the pinealectomy of the duck.


Levodopa and relief of Parkinsonian symptoms.


Induction of sporangia in Phytophthora cinnamomi by a substance from bacteria and soil.


Bacterial degradation of nitrilotriacetic acid (NTA).


Protein synthesis by cell-free extracts of Proteus vulgaris.


Association of chloramphenicol resistance of group A streptococci with host-controlled restriction and modification of bacteriophages.


Safety and efficacy of an attenuated Venezuelan equine encephalomyelitis vaccine for use in Equidae.


Effects of intra-articular injection of flumethasone suspension in joint diseases in horses.


Thrombosis of the corpus cavernosum penis in a bull.


Trichinosis decline continues.

Trichinosis decline continues.


Feline and canine dirofilarial encephalitis.


Constrictive pericarditis in two dogs.

Constrictive pericarditis in two dogs.


Effect of chloramphenicol on pentobarbital-induced anesthesia in dogs.


What is your diagnosis?

What is your diagnosis?


Exercise tests in patients with chronic disease.


Evaluating differences in survival between two groups of patients.


Professional team opinion and personal bias--a study of a chronic hemodialysis unit team.


Cardiovascular disease symptoms in Norway--a study of prevalence and a mortality follow-up.


Relationship of cerebrovascular disease to senile, presenile and cerebral arteriosclerotic dementia.


Anoxic brain damage after cardiac resuscitation.


Childhood malignancy.

Childhood malignancy.


Results of long-term home nursing: the influence of disability.


Retinal changes in toxemia of pregnancy.


Incidence of H.A.A. in blood donors at Chulalongkorn Hospital.


The determination of Australia antigen and antibody by cross-over immunoelectrophoresis.


Internship evaluation by questionnaire.

Internship evaluation by questionnaire.


Disseminated intravascular coagulation. Report of a case in a Thai infant and review of its pathogenesis.


Orbito-prosthesis post orbital exenteration. The first case report in Thailand.


Angiographic dissecting aneurysm of internal carotid artery. An autopsy case report.


Familial cirsoid aneurysm of the scalp.

Familial cirsoid aneurysm of the scalp. One brother and one sister, of seven siblings, had cirsoid aneurysms of the occipital scalp with underlying skull defects and possible intracranial communication. Another sister had no gross scalp abnormality but radiographs of the skull revealed a small occipital bony defect. This is thought to be the first reported example of familial cirsoid aneurysm of the scalp.


Analysis of L-dopa induced dyskinesias in 51 patients with Parkinsonism. An analysis of 51 patients with Parkinsonism who have developed L-dopa induced dyskinesias is presented. The cause has not been proven, although various hypotheses are discussed. One third of the total number of patients treated developed dyskinesia. These patients tend to respond better to L-dopa than the other group. There is a tendency for the older patient or the patient with long-standing disease to develop dyskinesias. There appears to be no way of predicting which patients will develop dyskinesia by analysis of the symptoms or the aetiology of the Parkinsonism syndrome. The unilateral characteristic of the dyskinesia in patients with hemi-Parkinsonism and patients with unilateral thalamotomies suggests that structural abnormalities are critical in determining the presence and localization of dyskinesias. This is supported by non-occurrence of similarly treated patients without Parkinsonism.


Blink reflex studies in patients with Parkinsonism before and during therapy. Electrophysiological analysis of the blink reflex makes it possible to quantitate the reflex changes observed in pathology. The authors therefore propose a `habituation index' to be determined by recording the electromyographic reflex response during stimulation at controlled frequencies. Several populations of subjects were tested. It was confirmed that marked differences in blink reflex habituation exist between normals and patients with Parkinson's disease. It was also found that when patients with Parkinsonism were treated with the classical anticholinergic drugs their habituation index remained virtually unchanged. However, treatment with either l-dopa or amantadine caused a rise in the index in almost all patients tested. On the basis of these findings it is suggested that these two new drugs have a highly similar mechanism of action.


Effect of L-dopa on speech in Parkinsonism. An EMG study of labial articulatory function. The articulatory function of the labial musculature has been investigated electromyographically before and after treatment with l-dopa in patients with Parkinsonism who had dysarthria. Before medication the EMG traces generally showed a constant, abnormally increased, tonic activity, together with disturbed reciprocal innervation, which impaired the articulatory activity. After medication the tonic hyperactivity was reduced and the reciprocal innervation re-established. This normalization of the EMG articulatory pattern was paralleled by an improvement of the dysarthria.


Effect of intracranial hypotension on cerebral blood flow. Intracranial hypotension increases cerebral blood flow. In dogs the average increase in cortical blood flow was 30 ml./100 g/min (47%) when the intracranial pressure was lowered acutely from 100 to 40 mm CSF. Permanent intracranial hypotension was established in seven demented patients using a ventriculoatrial shunt. The mean post shunt pressure was 50 mm CSF. In this group, the cerebral vascular resistance decreased 32%, the cortical blood flow increased 37%, and the relative weight of functional grey matter increased 44%. The systemic blood pressure was 8% lower. The increase in cerebral blood flow is the result of an increase in the pressure differential between the precapillary arterioles and the veins. In addition, the vessels dilate in response to the decreased external pressure. This increase in cerebral blood flow may be the mechanism for improvement in patients with normal pressure hydrocephalus who are shunted.


Failure of vitamin B6 to reverse the L-dopa effect in patients on a dopa decarboxylase inhibitor. Seven patients with Parkinsonism previously on l-dopa were placed on a regimen of l-dopa and alpha methyl dopa hydrazine (a dopa decarboxylase inhibitor). Two of these patients had previously shown marked clinical deterioration of the l-dopa improvement when given pyridoxine. None of the seven patients receiving alpha methyl dopa hydrazine demonstrated any change in their condition when given pyridoxine. The failure of vitamin B(6) to reverse the clinical effect of l-dopa in patients receiving both l-dopa and a peripheral dopa decarboxylase inhibitor suggests that reversal of the l-dopa effect induced by vitamin B(6) is due to increasing the activity of the enzyme dopa decarboxylase outside the central nervous system.


Akinetic mutism and bilateral anterior cerebral artery occlusion. Three cases of bilateral anterior cerebral artery occlusion are presented with akinetic mutism. The anatomical distribution of the infarction in these patients combined with cases in the literature suggests that this syndrome can have a localizing value for the clinician. If increased intraventricular pressure is not present, the clinician can suspect a bilateral lesion of cingulate gyrus, medial nuclei of basal ganglia, and/or anterior and reticular nuclei of the thalamus.


Potentiation of "late" responses evoked in muscles during effort. A study has been made of the effect of voluntary contraction on the `late' responses which can be recorded in muscles after indirect stimulation. Two late responses have been described of which the first was shown to consist mainly of a potentiated H-reflex. The potentiation of this wave was greatest in the abductor pollicis brevis muscle and depended largely on `direct' descending pathways to motoneurones. In addition, a later evoked muscle response appeared during effort; the nature of this wave was uncertain. Simple formulae have been devised to express the excitability of motoneurones to inputs from muscle nerves during effort.


Impaired potentiation of H-reflexes in patients with upper motoneurone lesions. The potentiation of H-reflexes during effort has been studied in 16 patients with mild hemiparesis. Impaired potentiation was present in all but one patient, and was still evident in some who had recovered fully from weakness. A tentative explanation has been proposed for these findings; it is suggested that there is loss of a background facilitatory pathway to motoneurones.


Malignant gliomas occurring in identical twins. The occurrence of malignant gliomas in a pair of identical twins is reported. One, in the posterior fossa, presented in the third year of life, the other, in the left cerebral hemisphere, in the eighth. Both ran a rapidly progressive course and died within a few months of the onset of symptoms. The histological structure of the gliomas was similar and both tumours consisted of astrocytic and oligodendroglial parts. Review of the literature revealed only two pairs of histologically proven gliomas occurring in identical twins. The number of cases reported is too small for any valid conclusions on possible genetic mechanisms.


Sphenoidal defects--a possible cause of cerebrospinal fluid rhinorrhoea. In a study of 138 adult sphenoidal bones, 27 defects which could cause cerebrospinal fluid rhinorrhoea were found. Fourteen of these defects were situated at the site of the superior opening of the transient lateral craniopharyngeal canal. The remaining 13 defects were situated along the pathway of the internal carotid artery. The gross and microscopic appearance of the defects is consistent with their production by a process of `focal atrophy' caused by the pressure of the internal carotid artery and other adjacent structures.


Some observations on electroencephalograms of myoclonus epilepsy. Electroencephalographic observations of four cases of myoclonus epilepsy are reported. A feature in EEG findings was that spikes became more prominent in the later stage of illness. Photic stimulation had a distinct activating effect on producing paroxysms, and an increase in number of paroxysms depended not on the frequency of stimulus but on the intensity. A sleep EEG showed ill-formed humps and sigma rhythm with infrequent paroxysms. It is suggested that these observations might be due to a disturbance in the function of subcortical structures.