Congenital megacolon (Hirschsprung's disease). Follow-up on a series from 40 years.


A new classification of the toxemia of pregnancy into four types.


Cholinesterase and active sodium transport in frog skin.


The pressure of the past [Gandhi].

The pressure of the past [Gandhi].


Structure of the closing mechanism of the conditioned reflex.


The interaction of different kinds of temporary connections in the process of forming conditioned reflexes to the relationship of stimuli.


Flammulin, a basic protein of Flammulina velutipes with antitumor activities.


The influence of the vascular reactions of the maternal uterus on the cardiac activity of the fetus.


Synthesis of some hydrazono derivatives of p-[N,N-bis(beta-chloroethyl)amino]benzaldehyde.


Multiplication dynamics of phase I and II Coxiella burneti in different cell cultures.


The relationship between secretion and incretion of pepsinogen.


Evaluation of spirometers used in pulmonary function studies.


Results of the treatment of haematogenous osteomyelitis with special reference to radical operation in connection with antibiotic and anticoagulant therapy.


Cirramycin, a new antibiotic.

Cirramycin, a new antibiotic.


A stereotaxic instrument for use in man.


Bacterial factors in shock.

Bacterial factors in shock.


Cervical cell inclusion bodies and viral infections of the cervix.


Oxygen tension in the canine brain under high-altitude conditions.


"Bugs 'n blood 'n rabbit fever ..."

"Bugs 'n blood 'n rabbit fever ..."


Skiing accidents and their prevention.

Skiing accidents and their prevention.


Relation of the internal carotid siphon to the carotid sulcus.


Age-dependent changes in mucosal glands of accessory nasal sinuses in man.


The pathogenesis of syringomyelia.

The pathogenesis of syringomyelia.


Participation of the reticular formations of the pons and medulla in vasomotor regulation.


"Secondary failures" in the treatment of diabetes mellitus with tolbutamide and with phenformin.


A thoracopagus with unusual cardiovascular anomalies.


Effect of cultivation temperature on sensitivity of infusoria to injurious action of X rays.


Anaphylaxis with orally administered penicillin.


Demonstration of Rickettsia prowazeki in cell cultures by the fluorescent antibody method.


Stasis and necrosis; a brief review.

Stasis and necrosis; a brief review.


A method of studying urinary excretion in large-horned cattle.


Consensual intraocular pressure changes resulting from unilateral extraocular muscle contraction.


Review lectures on senescence. II. Heterochronic transplantation in the study of ageing.


Antibodies formed in response to individual ionization states of benzenearsonic acid.


The early lesions in pulmonary emphysema.


Polyoma-induced tumors in mice. In vitro studies.


Chlamydospores and dimorphism in Candida albicans endocarditis. Observations in a fatal superinfection during treatment of staphylococcus endocarditis.


FREUD'S pathography and psychoanalysis.

FREUD'S pathography and psychoanalysis.


Henry HEAD'S work on sensation.

Henry HEAD'S work on sensation.


Hypernephroma in the young adult. Case reports.


A confusion of verbs; to use and to need.


Effect of drugs on the uptake and metabolism of H3-norepinephrine.


Chemical and morphological collagen changes in the course of 20-methylcholanthrene-induced carcinogenesis in mouse skin.


Idiopathic polyserositis.

Idiopathic polyserositis.


Nathan SMITH's medical practice or dogmatism versus patient inquiry.


Respiratory abnormalities in eosinophilic granuloma of the lung. Longterm study of five cases.


Sex differences, sex composition, and group problem solving.


Differences and disagreement as factors in creative group problem solving.


A note on the origins of ego psychology.


On the relationship between psychoanalysis and the philosophy of mind.


The importance of pneumoperitoneum.

The importance of pneumoperitoneum.


Assessment of the independent contributions of predictors.


Simple method for quantitation of A2 hemoglobin fraction.


The influence of exercise on the growth of transplanted rat tumors.


Successful long-distance aerial transport of fertilized sheep ova.


A privately printed pamphlet by Sir Charles BELL on the principles involved in appointments to the London hospitals.


Aerosol immunization with dried live vaccines and toxoids. VII. Organization, methods and technique of massive aerosol immunization of man with dust vaccines.


Cancer of the ovary and its treatment.

Cancer of the ovary and its treatment.


Decrease of radiation damage to proteins by sulfhydryl protectors.


A new method for the turbidimetric measurement of bacterial density. Kurokawa, Masami (National Institute of Health, Tokyo, Japan), Motoichi Hatano, Noboru Kashiwagi, Tateo Saito, Setsuji Ishida, and Reiko Homma. A new method for the turbidimetric measurement of bacterial density. J. Bacteriol. 83:14-19. 1962.-It was demonstrated that by a combination of two types of metameter of transmittance (T), -log T (0.0 </= T </= 0.5) and 0.6 + log (1 - T) (0.5 </= T </= 1.0), the optical density thus expressed showed a linear relationship to bacterial density or cell concentration over the whole range of T, except at the extreme ends. This allows measurement of relative bacterial density and cell concentration, and eliminates unnecessary sampling and dilution. In this respect, the proposed method seems superior to the method based upon Beer's law. The possible error derived from the fact that organisms may vary in size and shape at different stages of their growth is a fault common to all known methods of turbidimetric measurement, and is not peculiar to the method presented here.


Trichlorethylene-saturated water and tetrachloroethylene chemical dosimeter systems-neutron response.


Studies on the metabolism of Shigella. IV. Nutritional requirements of Shigella flexneri serotype 3. Pan, Sylvia F. (University of Pittsburgh, Pittsburgh, Pa.), Robert B. Yee, and Horace M. Gezon. Studies on the metabolism of Shigella. IV. Nutritional requirements of Shigella flexneri serotype 3. J. Bacteriol. 83:61-65. 1962.-A study of the nutritional requirements of five strains of Shigella flexneri serotype 3 was carried out. Four of the strains could be cultured in a synthetic medium composed of a mineral salts glucose base, NH(4)H(2)PO(4) or an amino acid as the nitrogen source, and nicotinamide; the fifth one required the presence of both NH(4)H(2)PO(4) and an amino acid in the medium. Variation among the former strains in their nitrogen and vitamin requirements was observed. The least fastidious one produced maximal growth using the simple ammonium salt as its source of nitrogen and did not require the addition of nicotinamide. Two strains were moderately fastidious, producing better growth within 24 hr in the presence of l-aspartic acid instead of NH(4)H(2)PO(4), and requiring nicotinamide as an accessory growth factor. The fourth strain was extremely fastidious, growing poorly or not at all in the synthetic medium. Incubation of large inocula of the fastidious strain in synthetic medium and over a prolonged period resulted in adaptation of the organism to the medium. Some of the adapted cultures grew readily in the presence of NH(4)H(2)PO(4) and did not require nicotinamide.


Army Medical Corps first with reservists and foremost in World War I preparedness.


The antigenicity of the Rh-o (Du) blood factor.


A simplified procedure for performing urinary bacterial counts.


Isolation and properties of Beggiatoa.

Isolation and properties of Beggiatoa.


Complications of gynecologic surgery. Post-operative water, electrolyte, and transfusion therapy.


The effect of aerobiosis and diphenylamine on the content of ubiquinone in Rhodospirillum rubrum. Biochim Biophys.


Progressing from "control" to eradication of tuberculosis. the need for research into the natural history of tuberculosis.


The toxicity of vinyl chloride as determined by repeated exposure of laboratory animals.


Acute myocardial infarction revealed in the presence of right bundle branch block and ventricular extrasystoles.


Cecal haustrum intussusception. Report of 9 cases.


Urethral leiomyoma protruding into the vagina. Report of a case.


Concepts in developing a food composition table.


Chemical structure and fibrinolysis induction.


Displaced persons in Germany.

Displaced persons in Germany.


A new oral hypoglycemic agent.

A new oral hypoglycemic agent.


The lente insulin triad, with emphasis on the use of "lente combinations".


Ambulatory treatment of tuberculosis.

Ambulatory treatment of tuberculosis.


Sterilization of instruments and dressings in general practice.


Role of vitamin K in electron transport.


An adaptive device for temporary above-knee prosthesis.


Studies on the origin of visceral malformation in centrifuged eggs of a toad.


Influence of the aqueous potato extract and its fractions on growth and spore formation of the B pumilus and the production of the antibiotic tetaine.


Olfactory and visual learning in the red-eared terrapin, Pseudemys scripta elegans (Wied.).


Studies on an artificial heart controlled by a venous pressure servomechanism.


An approach to basic research in podiatry.


The enzymatic synthesis of sialyl-lactose.


Study of intestinal helminth infections in a coastal South Carolina area.


Simplified approach to otitis externa.

Simplified approach to otitis externa.


The family doctor at the wheel. New cars tested at Goodwood.


Exceptional students in nursing.

Exceptional students in nursing.


Presidential address.

Presidential address.


Whole pancreas as an adjuvant in wound debridement.


Neonatal problems in West Africa.

Neonatal problems in West Africa.


Prolonged breathing of a gas mixture high in O2 and low in CO2 at rest and during exercise.


Analysis of the therapeutic efficacy of dihydrostreptomycin and tetracycline against experimental tularemic infections in mice.


Lysis of isolated BCG cell walls with enzymes.


FINE STRUCTURE OF THE ACINAR CELL IN THE HAMSTER BULBOURETHRAL GLAND.


QUANTITATIVE STUDIES ON BONE RECONSTRUCTION. I. THE RECONSTRUCTION IN HOMOTYPIC SHAFT BONES.


HISTOCHEMICAL STUDIES ON THE DISTRIBUTION OF MONOAMINE OXIDASE IN THE CEREBELLUM OF RAT.


FREQUENCY VARIATIONS OF "DRUMSTICKS" OF PERIPHERAL BLOOD NEUTROPHILES IN THE RABBIT IN DIFFERENT ALIMENTARY CONDITIONS.


Separation and determination of thiamine phosphates in animal tissues by starch block electrophoresis.


CAUSE AND EFFECT OF WEAR OF TEETH. FURTHER NON-METRICAL STUDIES OF THE TEETH AND PALATE IN MORIORI AND MAORI SKULLS.


THE ELECTROPHORETIC BEHAVIOR OF HUMAN AND CANINE HAEMOGLOBIN AND THEIR RECOMBINATION PRODUCTS.


HAEMATOLOGICAL CONCEPTS OF MONOMORPHISM, POLYMORPHISM AND THE BONE MARROW-BLOOD BARRIER IN THE LIGHT OF THE INFORMATION THEORY.


PAPILLOEDEMA DUE TO IRON DEFICIENCY ANAEMIA.


SPLENECTOMY IN TWO CASES OF HAEMOGLOBIN Q-H-DISEASE (HB Q-ALPHA-THALASSAEMIA).


INTESTINAL EXCRETION OF URIC ACID IN DOGS.


STUDIES ON THE ONCOGENIC ACTIVITY OF POLYOMA VIRUS IN VITRO IN VIVO. III. MALIGNANT TRANSFORMATION OF HAMSTER HEART CELLS AS RESULT OF POLYOMA VIRUS INFECTION.


ON SOME ASPECTS OF THE ANTIBODIES  DEFICIENCY SYNDROME.


SERUM VITAMIN B12 DETERMINATIONS AND CYTOCHEMICAL REACTIONS IN THE DIFFERENTIAL DIAGNOSIS OF ACUTE  LEUKAEMIA.


COEXISTING HYPERTHYROIDISM AND HYPERPARATHYROIDISM.


INFECTIOUS RENAL DISEASE IN A  DEPARTMENT OF GENERAL MEDICINE. A SURVEY OF CASE NOTES FROM 11 YEARS.


GLYCOGEN CONTENT OF LEUKOCYTES AND PLATELETS.


ADRENOCORTICAL INSUFFICIENCY DUE TO METASTATIC INFILTRATION OF THE ADRENAL GLANDS.


THE BASOPHIL LEUKOCYTES IN PATIENTS WITH BLEEDING PEPTIC ULCER.


RETICULOCYTOSIS IN CONGESTIVE HEART FAILURE.


ALPHA-METHYLDOPA IN ARTERIAL HYPERTENSION: CLINICAL, RENAL AND HEMODYNAMIC STUDIES.


CONGENITAL RENAL DISEASE, DEAFNESS AND MYOPIA IN ONE FAMILY.


POLYCYTHAEMIA IN ANOXIC PATIENTS WITH CIRCULATORY FAILURE.


DISAPPEARANCE OF WAXY EXUDATES IN DIABETIC RETINOPATHY DURING ADMINSTRATION OF P-AMINO-SALICYLATE (PAS).


THE BECK-I OPERATION FOR ANGINA PECTORIS: MEDICAL ASPECTS.


NEED OF FUNCTIONAL ANALYSIS AND SELECTIVE GRINDING IN ORTHODONTICS. A CLINICAL AND ELECTROMYOGRAPHIC STUDY.


STUDY OF THE SETTING EXPANSION OF GYPSUM.


THE GRAIN SIZE OF ZINC PHOSPHATE CEMENTS.


STUDIES IN ORAL LEUKOPLAKIAS. III. EFFECTS OF VITAMIN A COMPARING CLINICAL, HISTOPHATHOLOGIC, CYTOLOGIC, AND HEMATOLOGIC RESPONSES.


COMPARATIVE EFFECTS OF INTRA-ARTERIALLY ADMINISTERED HYDRALAZINE AND SODIUM NITRITE ON BLOOD FLOW AND VOLUME OF FOREARM.


THE EFFECT OF SODIUM FLUORIDE ON THE ACTIONS OF SUCCINYLCHOLINE, PARATHION AND DEMETON IN RATS.


SUPERSENSITIVITY TO ETHANOL IN RABBITS TREATED WITH COPRINUS ATRAMENTARIUS.


ON THE ROLE OF THE LIVER CATECHOL-O-METHYL TRANSFERASE IN THE METABOLISM OF CIRCULATING CATECHOLAMINES.


THE COMPARATIVE ABSORPTION OF CREATININE FROM "GITTER" TABLETS AND CONTROL TABLETS.


INVESTIGATIONS INTO THE PROPERTIES OF A SUBSTANCE CLAIMED TO INCREASE ABSORPTION (UBIQUIN).


PROTECTION BY INDIGENOUS DRUGS AGAINST HEPATOTOXIC EFFECTS OF CARBON TETRACHLORIDE IN MICE.


THE LETHAL ACTION OF MORPHINE AND NALORPHINE GIVEN JOINTLY TO MORPHINE TOLERANT AND NON-TOLERANT RATS.


MONOAMINE OXIDASE INHIBITION AND SENSITIVITY OF THE NICTITATING MEMBRANE TO NORADRENALINE.


RENAL FUNCTION IN RHEUMATOID ARTHRITIS.

RENAL FUNCTION IN RHEUMATOID ARTHRITIS.


ASYMMETRY OF OSSIFICATION AND RATE OF GROWTH OF LONG BONES IN CHILDREN WITH UNILATERAL JUVENILE GONARTHRITIS.


THE KIDNEY FUNCTION IN RELATION TO DRUG CONSUMPTION IN PATIENTS WITH RHEUMATOID ARTHRITIS. (V).


CORNEAL CHANGES DURING CHLOROQUINE TREATMENT. AN INVESTIGATION OF 70 PATIENTS WITH CHRONIC RHEUMATOID ARTHRITIS RECEIVING CHLOROQUINE TREATMENT.


POLYMYALGIA RHEUMATICA. A FORM OF SENILE ARTERITIS?


A SUSPENSION OF KAOLIN PARTICLES USED AS A MEDIUM TO DETECT THE RHEUMATOID FACTOR IN BLOOD SERUM.


ISOTOPE STUDIES OF GAMMAGLOBULIN CATABOLISM IN COLLAGEN DISORDERS.


EFFECTS OF RESPIRATORY INHIBITORS ON DEVELOPMENT OF THE DOWN FEATHER.


CURRENT PROBLEMS AND NEW DEVELOPMENTS IN RESPIRATORY PROTECTION.


EFFERENT CONNECTIONS OF THE BASAL AND LATERAL NUCLEI OF THE AMYGDALA IN THE CAT.


EXPERIMENTAL CARCINOGENICITY AND ACUTE TOXICITY OF REPRESENTATIVE EPOXIDES.


MITOCHONDRIAL CHANGES IN DEVELOPING NEURONS.


SEASONAL CHANGES IN BODY WEIGHT, REPRODUCTIVE ORGANS, PITUITARY, ADRENAL GLANDS, THYROID GLAND, AND SPLEEN OF THE BELDING GROUND SQUIRREL (CITELLUS BELDINGI).


BLOOD VESSELS OF THE CHICKEN'S UTERUS (SHELL GLAND).


THE EFFECTS OF SHORT EXPOSURES TO TRICHLOROETHYLENE ON SWIMMING PERFORMANCES AND MOTOR ACTIVITY OF RATS.


DISCUSSION OF PROPOSED CHANGES IN STANDARDS FOR HEARING TESTING INSTRUMENTS.


AN AUDIOMETRIC APPROACH TO NOISE CONTROL.


SERTOLI CELLS AND TESTIS STRUCTURE.

SERTOLI CELLS AND TESTIS STRUCTURE.


THE STRUCTURE OF INTERMEDIATE VASCULAR PATHWAYS IN THE SPLEEN OF RABBITS.


HISTOCHEMICAL STUDIES ON THE SKIN. I. THE ACTIVITY OF PHOSPHATASES DURING THE HISTOGENESIS OF THE SKIN IN THE RAT.


THE IRRITANT POTENCY OF ZINC AMMONIUM SULFATE OF DIFFERENT PARTICLE SIZES.


THE VASCULAR SUPPLY TO THE CENTRAL NERVOUS SYSTEM OF THE LARVAL LAMPREY.


HYGIENIC GUIDE SERIES.

HYGIENIC GUIDE SERIES.


MORPHOGENETIC STUDIES OF THE RABBBIT. 32. QUALITATIVE SKELETAL VARIATIONS INDUCED BY THE AC GENE (ACHONDROPLASIA).


CONGENITAL ANEURYSM OF THE SINUS OF VALSALVA. CRITERIA FOR RECOMMENDING SURGERY.


PYRIDINE.

PYRIDINE.


THROMBOANGIITIS OBLITERANS: A TRUE CLINICAL ENTITY.


CRITERIA FOR SURGERY IN MITRAL HEART DISEASE.


PETROLEUM NAPHTHA.

PETROLEUM NAPHTHA.


THE FORWARD TRIANGLE FORMULA FOR CALCULATIONS OF CARDIAC OUTPUT. THE INDICATOR-DILUTION TECHNIC.


BEHAVORIAL CHANGES IN THE PIGEON FOLLOWING INHALATION OF MERCURY VAPOR.


A CLASSIFICATION OF CONGENITAL HEART DISEASE. A PHYSIOLOGIC APPROACH.


CRITERIA FOR SURGERY IN MITRAL VALVULAR DISEASE.


THE RESPONSIBILITY OF THE CARDIOLOGIST IN RECOMMENDING CARDIAC SURGERY.


INDICATIONS FOR OPEN OPERATIONS FOR MITRAL STENOSIS.


CRITERIA FOR SURGERY IN AORTIC VALVULAR DISEASE.


CRITERIA FOR OPEN HEART SURGERY FOR ACQUIRED AORTIC VALVULAR DISEASE.


THE OPTIMAL TIME FOR CARDIAC SURGERY.

THE OPTIMAL TIME FOR CARDIAC SURGERY.


OBSTRUCTION TO LEFT VENTRICULAR OUTFLOW. CURRENT CRITERIA FOR THE SELECTION OF PATIENTS FOR OPERATION.


INDICATIONS FOR THE SURGICAL CORRECTION OF COARCTATION OF THE AORTA IN INFANCY.


INDICATIONS FOR REPAIR OF VENTRICULAR SEPTAL DEFECTS.


NATURAL HISTORY OF ATRIAL SEPTAL DEFECT WITH CRITERIA FOR SELECTION FOR SURGERY.


CRITERIA FOR SURGERY IN PERSISTENT COMMON ATRIOVENTRICULAR CANAL.


TETRALOGY OF FALLOT--INDICATIONS FOR OPERATION.


CRITERIA FOR RECOMMENDING SURGERY IN TOTAL ANOMALOUS PULMONARY VENOUS DRAINAGE.


CRITERIA FOR RECOMMENDING SURGERY IN THE TETRALOGY OF FALLOT.


THE HEALTH INSURANCE PLAN--NEW YORK CITY WELFARE DEPARTMENT PROJECT.


PUBLIC HEALTH EDUCATORS: A STUDY OF AVAILABILITY AND DISTRIBUTION OF ALUMNI--  WITH IMPLICATIONS FOR THE PROFESSION.


AN EVALUATION OF COMMUNITY NURSING SERVICE IN THE CARE OF THE MENTALLY ILL. II. ADMINISTRATIVE PROBLEMS AND EXPERIENCE.


AN EXPERIMENTAL STUDY OF TWO APPROACHES TO COMMUNICATION.


PESTICIDE POISONING ACCIDENTS AMONG YOUNG CHILDREN.


PROBLEMS IN MANUFACTURING AND MARKETING PESTICIDES.


FEDERAL AND STATE REGULATION OF PESTICIDES.


RESEARCH IN TOXICOLOGY OF PESTICIDES.

RESEARCH IN TOXICOLOGY OF PESTICIDES.


OCCUPATIONAL HEALTH PROBLEMS POSED BY AGRICULTURAL PESTICIDES.


PESTICIDE RESIDUES.

PESTICIDE RESIDUES.


NEW DIRECTIONS IN ENVIRONMENTAL HEALTH.

NEW DIRECTIONS IN ENVIRONMENTAL HEALTH.


SOME PROBLEMS OF STRATEGY IN PROVIDING SCHOOL MENTAL HEALTH SERVICES.


CONSIDERATION AND USES OF HYPERBARIC OXYGEN THERAPY IN SURGERY.


EXPERIENCE WITH THE SURGICAL MANAGEMENT OF 100 CONSECUTIVE CASES OF ABDOMINAL AORTIC ANEURYSM.


ACUTE ARTERIAL INJURIES OF THE UPPER EXTREMITY.


PYOGENIC INFECTIONS IN ORTHOPAEDIC SURGERY. COMBINED ANTIBIOTIC AND CLOSED WOUND TREATMENT.


CONTINUOUS VENOUS PRESSURE MONITORING AS A GUIDE TO FLUID ADMINISTRATION IN THE HYPOTENSIVE PATIENT.


SURGICAL EXPERIENCE WITH PEPTIC ULCER IN INFANCY AND CHILDHOOD.


GASTRIC RESECTION FOR DUODENAL ULCER.

GASTRIC RESECTION FOR DUODENAL ULCER.


THE USE OF CATHETER DUODENOSTOMY IN GASTRIC RESECTION FOR DUODENAL ULCER.


COMPLICATIONS OF VAGOTOMY.

COMPLICATIONS OF VAGOTOMY.


MALIGNANT LYMPHOMA OF THE STOMACH.

MALIGNANT LYMPHOMA OF THE STOMACH.


AN EXPERIMENTAL STUDY OF SELECTIVE RENAL HYPOTHERMIA.


HEMORRHAGIC SHOCK AND THE NERVOUS SYSTEM.


TREATMENT OF CHRONIC PANCREATITIS BY LONGITUDINAL PANCREATICOJEJUNOSTOMY.


THE SURGERY OF CONGENITAL DEFECTS OBSTRUCTING THE OUTFLOW OF THE RIGHT VENTRICLE.


TOTAL SURGICAL CORRECTION OF FALLOT'S ANOMALY.


METABOLIC OBSERVATIONS IN PATIENTS WITH JEJUNOCOLIC SHUNTS.


TRACHEOSTOMY: PRESENT DAY INDICATIONS AND TECHNICS.


MAMMOGRAPHY IN THE DIAGNOSIS OF CANCER OF THE FEMALE BREAST.


THE DISAPPEARING ENZYMES. A STUDY OF PANCREATIC ENZYMES IN NORMAL PATIENTS AND AFTER BILLROTH I AND II GASTRIC RESECTIONS.


THE PRESENT STATUS OF INTERNAL MAMMARY NODE DISSECTION FOR CARCINOMA OF THE BREAST.


GASTROCOLIC FISTULAE OF MALIGNANT ORIGIN. A CONSIDERATION OF ITS NATURE AND REPORT OF FIVE CASES.


ILEORECTAL ANASTOMOSIS FOR ULCERATIVE COLITIS.


THE PROBLEM OF COMPLETE HEART BLOCK.

THE PROBLEM OF COMPLETE HEART BLOCK.


SURGICAL MANAGEMENT OF COLON TRAUMA IN CIVILIAN PRACTICE.


EARLY AND LATE COMPLICATIONS OF THE SWENSON PULL-THROUGH OPERATION FOR HIRSCHSPRUNG'S DISEASE.


METHODS OF APPROACH TO LOW LYING LESIONS IN THE RECTUM.


EFFECT OF HYPOTHERMIA ON EXPERIMENTAL ACUTE HEMORRHAGIC PANCREATITIS.


PRIMARY MALIGNANT NEOPLASMS OF THE DUODENUM: REPORT OF TWELVE CASES.


THE HAND IN RHEUMATOID ARTHRITIS.

THE HAND IN RHEUMATOID ARTHRITIS.


VILLOUS TUMORS OF THE COLON AND RECTUM.

VILLOUS TUMORS OF THE COLON AND RECTUM.


RATIONAL SURGICAL MANAGEMENT OF ESOPHAGEAL HIATAL HERNIA.


SURGICAL TREATMENT OF PORTAL HYPERTENSION IN INFANTS AND CHILDREN.


URINARY TRACT COMPLICATIONS OF PELVIC FRACTURES.


TUMORS OF THE ULNAR AND MEDIAN NERVES.

TUMORS OF THE ULNAR AND MEDIAN NERVES.


SURGICAL TREATMENT OF BENIGN AND MALIGNANT CAROTID BODY TUMORS: CLINICAL EXPERIENCE WITH SIXTEEN TUMORS IN TWELVE PATIENTS.


INGUINAL HERNIA OF THE INTERNAL GENITALIA IN FEMALE INFANTS AND CHILDREN.


EFFECT OF 20 PER CENT CHOLESTEROL DIET ON SEGMENTAL EXPERIMENTAL ATHEROGENESIS.


CARCINOMA OF THE STOMACH IN THIS COUNTRY AND IN THE ORIENT.


LOCALIZED INTRA-THORACIC LYMPHOMAS.

LOCALIZED INTRA-THORACIC LYMPHOMAS.


EXPERIENCE WITH EMULSION THERAPY IN A LARGE MEDICAL GROUP. II.


HUMORAL RESPONSE TO ALLERGENS OF LOW MOLECULAR WEIGHT.


VENOM SAC EMULSION. PRELIMINARY REPORT.

VENOM SAC EMULSION. PRELIMINARY REPORT.


WITH WHAT WE HAVE TO CONTEND.

WITH WHAT WE HAVE TO CONTEND.


AIR POLLUTION AND RESPIRATORY DISEASE.

AIR POLLUTION AND RESPIRATORY DISEASE.


A COMPARISON OF THE RELEASE OF I-131 FROM SALINE, REPOSITORY, AND VITELLINIC INJECTION SITES.


ADVERSE REACTIONS IN 3000 EMULSION INJECTIONS AND INTRODUCTION OF A NEW NON-METALLIC EMULSATOR.


A SEVERE SYSTEMIC REACTION TO DEMEROL. CASE REPORT.


OPERATIVE TREATMENT OF VARICOSE SYNDROMES OF THE LOWER EXTREMITY WITH THE AID OF VENOGRAPHY.


THE CLINICAL APPLICATION OF VASO-SEMINAL VESICULOGRAPHY IN DIFFERENTIAL DIAGNOSIS OF PROSTATIC HYPERTROPHY AND CANCER.


VOLUME DOSE AND GONAD IN  X-RAY DIAGNOSIS.


SCALENE NODE BIOPSY.

SCALENE NODE BIOPSY.


ALDOSTERONE SECRETION AND VARIOUS FORMS OF HYPERTENSIVE VASCULAR DISEASE.


PREGNANCY AND DIABETES.

PREGNANCY AND DIABETES.


OBSERVATIONS ON METABOLISM OF ALDOSTERONE IN MAN.


HYPERALDOSTERONISM AND HYPERGRANULARITY OF THE JUXTAGLOMERULAR CELLS IN RENAL HYPERTENSION. METABOLIC STUDIES.


EXPERIMENTAL PYELONEPHRITIS AND HYPERTENSION. IMPLICATIONS FOR THE CLINICAL PROBLEM.


A RE-EVALUATION OF NEEDLE BIOPSY IN THE DIAGNOSIS OF METASTATIC CANCER OF THE LIVER.


LIVER FUNCTION TESTS AND NEEDLE BIOPSY IN THE DIAGNOSIS OF METASTATIC CANCER OF THE LIVER.


TREATMENT OF ADRENOCORTICAL CANCER WITH O,P'-DDD.


"PITUITARY RESERVE" IN MYXEDEMA AND THYROTOXICOSIS.


PRIMARY CUTANEOUS COCCIDIOIDOMYCOSIS. VISIBLE CLASSIC DEMONSTRATION OF DELAYED HYPERSENSITIVITY.


THE GRANULARITY OF THE JUXTAGLOMERULAR CELLS IN HUMAN HYPERTENSION. HISTOLOGIC AND CLINICAL CORRELATIONS.


RECURRENT HYPOGLYCEMIA ASSOCIATED WITH ACUTE ALCOHOLISM.


GONOCOCCAL MENINGITIS COMPLICATING TREATED GONOCOCCAL URETHRITIS.


SARCOID REACTIONS ASSOCIATED WITH PHENYLBUTAZONE HYPERSENSITIVITY.


CYTOPHYSICAL STUDIES ON LIVING NORMAL AND NEOPLASTIC CELLS.


ELECTRICAL STIMULATION OF THE HEART.

ELECTRICAL STIMULATION OF THE HEART.


OBSERVATIONS ON INTRAHEPATIC VASCULAR PRESSURE REGULATION IN PORTAL HYPERTENSION.


CIRCULATING FIBRINOLYTIC ACTIVATOR AND HEMORRHAGIC DIATHESIS.


TOTAL REMOVAL OF THE INTRATHORACIC ESOPHAGUS AND ANTETHORACIC JEJUNAL ESOPHAGEAL REPLACEMENT FOR TREATMENT OF ESOPHAGEAL  VARICES DUE TO EXTRAHEPATIC PORTAL BLOCK.


POST-TRAUMATIC INTERNAL MAMMARY ARTERIOVENOUS FISTULA: DIAGNOSTIC CONSIDERATIONS AND REPORT OF A CASE.


ROTATIONAL OBSTRUCTION OF THE VERTEBRAL ARTERY DUE TO REDUNDANCY AND EXTRALUMINAL CERVICAL FASCIAL BANDS.


EMERGENCY THROMBO-ENDARTERECTOMY OF THE SUPERIOR MESENTERIC ARTERY: REPORT OF FOUR CASES.


EXFOLIATIVE CYTOLOGY AND EARLY CARCINOMA OF THE STOMACH.


UNILATERAL HEPATIC DUCT OBSTRUCTION.

UNILATERAL HEPATIC DUCT OBSTRUCTION.


SUBCUTANEOUS EMPHYSEMA FROM PERFORATED GASTRIC ULCER: CASE REPORT AND REVIEW OF LITERATURE.


SIGNIFICANCE OF PROLONGED ELEVATION OF THE SERUM AMYLASE.


CLOSTRIDIAL INFECTION FOLLOWING ABDOMINAL SURGERY.


HEPATIC REGENERATION IN THE NORMAL AND NECROTIC LIVER OF THE DOG.


SIGNIFICANCE OF LOCATION AND NUMBER OF AXILLARY METASTASES IN CARCINOMA OF THE BREAST.


BYPASS FOR MALIGNANT OBSTRUCTION OF THE ESOPHAGUS.


SURVIVAL AFTER SURGICAL TREATMENT OF CARCINOMA OF THE STOMACH.


CARCINOMA IN HETEROTOPIC GASTRIC PANCREAS.


LOCALIZED TISSUE HEATING APPARATUS FOR CANCER CHEMOTHERAPY.


BULLET EMBOLISM TO THE RIGHT AXILLARY ARTERY FOLLOWING GUNSHOT WOUND OF THE HEART.


AN UNUSUAL COMPLICATION OF MECKEL'S DIVERTICULA SEEN IN OLDER PATIENTS.


CORRECTION OF DEFECT IN FREE WATER EXCRETION IN POSTOPERATIVE PATIENTS BY EXTRACELLULAR FLUID VOLUME EXPANSION.


BILATERAL PULMONARY RESECTION FOR TUBERCULOSIS.


PYRAZINAMIDE COVERAGE AT THE TIME OF PULMONARY RESECTION FOR TUBERCULOSIS.


THYMECTOMY FOR MYASTHENIA GRAVIS.

THYMECTOMY FOR MYASTHENIA GRAVIS.


LEFT VENTRICULAR-RIGHT ATRIAL COMMUNICATION.


A REVIEW OF THE GENUS ECHINOCOCCUS RUDOLPHI, 1801.


ECHINOCOCCUS INFECTIONS IN MAN AND ANIMALS IN KENYA.


HETEROPNEUSTES FOSSILIS (BLOCH), A POSSIBLE AGENT FOR THE BIOLOGICAL CONTROL  OF THE SNAIL HOSTS OF SCHISTOSOMES.


AN ASSESSMENT OF THE INSECTICIDAL CONTROL OF SIMULIUM DAMNOSUM THEOBALD IN  ABUJA EMIRATE, NORTHERN NIGERIA, FROM 1955 TO 1960. I. THE EFFECT ON THE PREVALENCE OF  ONCHOCERCIASIS IN THE HUMAN POPULATION.


STUDIES ON THE CONTROL OF THE VECTORS OF LOIASIS IN WEST AFRICA. III. COMPARISON OF THE TOXICITIES OF DDT, DIELDRIN, ALDRIN AND GAMMA-BHC TO LATE-INSTAR  TABANID LARVAE.


THE BIONOMICS OF CHRYSOPS SILACEA AUSTEN, 1907. II. THE BITINGRHYTHM AND DISPERSAL IN RAIN-FOREST.


OBSERVATIONS ON NULLIPAROUS AND PAROUS RATES IN A POPULATION OF  ANOPHELES FUNESTUS IN EAST AFRICA.


SEROLOGICAL IDENTIFICATION OF THE NATURAL HOSTS  OF AEDES AEGYPTI (L.) AND SOME OTHER MOSQUITOES (DIPTERA, CULICIDAE) CAUGHT RESTING IN  VEGETATION IN KENYA AND UGANDA.


OBSERVATIONS ON A CLOSE ASSOCIATION  BETWEEN GLOSSINA TACHINOIDES AND DOMESTIC PIGS NEAR NSUKKA, EASTERN NIGERIA. I.-TRYPANOSOMA CONGOLENSE AND T. BRUCEI INFECTIONS IN THE PIGS.


COMPARATIVE UTILIZATION OF CARBOHYDRATES BY CULTURE FORMS OF TRYPANOSOMA  (SCHIZOTRYPANUM) CRUZI AND T. RANARUM.


STUDIES ON THE DISTRIBUTION AND TRANSMISSION OF MALARIA  AND FILARIASIS AMONG ABORIGINES IN MALAYA.


PHYLOGENETIC SIGNIFANCE OF THE SKIN OF MAN.


EXISTENCE OF RESERVOIR IN THE STRATUM CORNEUM. EXPERIMENTAL PROOF.


BLOOD COAGULATION IN EPIDERMOLYSIS BULLOSA HEREDITARIA. GENETIC OBSERVATIONS.


STUDY OF GALVANIC SKIN RESPONSE IN PSORIASIS.


HEREDITY AND PSORIASIS. STUDY OF A LARGE FAMILY.


BRADYKININ. SELECTED REVIEW OF THE LITERATURE: DERMATOLOGICAL ASPECTS.


MERCURIAL PIGMENTATION OF NAILS.

MERCURIAL PIGMENTATION OF NAILS.


TETRACYCLINE PIGMENTATION OF TEETH.

TETRACYCLINE PIGMENTATION OF TEETH.


THE COURSE IS CHARTED.

THE COURSE IS CHARTED.


ORAL AMPHOTERICIN B THERAPY IN BLASTOMYCOSIS.


PLASTIC ACIDS FOR OCCLUSIVE THERAPY.

PLASTIC ACIDS FOR OCCLUSIVE THERAPY.


TREATMENT OF PAINFUL AND DEEP FISSURES OF FEET. A NEW TECHNIQUE.


THE INFLUENCE OF GEORGRAPHY ON DUODENAL ULCERS.


PEPTIC ULCER IN AFRICA.

PEPTIC ULCER IN AFRICA.


ABSENT LEFT PULMONARY ARTERY AND RIGHT-SIDED PATENT DUCTUS ARTERIOSUS.


THE URINE SEDIMENT IN ACUTE APPENDICITIS.


MOHS' CHEMOSURGERY TECHNIQUE FOR BASAL CELL CARCINOMA OF THE CHIN AND CHEEK AREAS OF THE FACE.


MANAGEMENT OF JUXTARENAL AORTIC OCCLUSIONS.


COMPARISON OF METHODS USED TO DIAGNOSE RENAL HYPERTENSION. RENAL ARTERIOGRAPHY VERSUS INDIVIDUAL URETERAL CATHETERIZATION.


SURGICAL TREATMENT OF VENTRICULAR ANEURYSM. REPORT OF TEN CASES.


DIRECT AUSCULTATION OF THE HEART.

DIRECT AUSCULTATION OF THE HEART.


UNILATERAL THYROID NODULES WITH LYMPHOCYTIC THYROIDITIS. SURGICAL PROBLEMS AND MANAGEMENT.


ASEPTIC BARRIERS.

ASEPTIC BARRIERS.


PATHOGENESIS OF ESOPHAGEAL VARIX RUPTURE. A STUDY BASED ON GROSS AND MICROSCOPIC EXAMINATION OF THE ESOPHAGUS AT THE TIME OF BLEEDING.


BLOOD AMMONIA REDUCTION BY POTASSIUM EXCHANGE RESIN. EXPERIMENTATION IN ECK-FISTULA DOGS.


"DIRECTIONAL" FLOW PATTERNS IN PORTAL HYPERTENSION. ASSESSMENT BY SPLEEN PRESSURE AND PERCUTANEOUS SPLENOPORTOGRAPHY.


SIGNIFICANCE OF POST-TRAUMATIC AND POSTOPERATIVE OLIGURIA.


MYOCARDIAL CONTRACTILE FORCE IN EXPERIMENTAL HEMORRHAGIC SHOCK. SOME OBSERVATIONS.


REGIONAL BLOOD FLOW AND OXYGEN CONSUMPTION IN EXPERIMENTAL HEMORRHAGIC SHOCK.


STUDIES ON VISCOSITY ALTERATIONS IN SHOCK. I. EFFECT OF HIGH AND LOW VISCOSITY DEXTRANS UPON PLASMA AND RED CELL VOLUMES.


PALLIATIVE CHEMOTHERAPY FOR THE CANCER PATIENT.


UNUSUAL LESIONS OF THE JEJUNUM AND ILEUM.


RESEARCH IN THE CONNECTIVE TISSUE DISEASES.


THE INHIBITION OF ACETYL COA CARBOXYLASE BY LONG CHAIN ACYL COA DERIVATIVES.


BRONCHIAL CARCINOMA AND SARCOIDOSIS.

BRONCHIAL CARCINOMA AND SARCOIDOSIS.


DIFFERENCES IN BIOLOGICAL CHARACTERISTICS OF VARIOUS HISTOLOGICAL TYPES  OF LOWER RESPIRATORY TRACT TUMOURS.


THE HISTOLOGICAL CLASSIFICATION OF LOWER RESPIRATORY TRACT TUMOURS.


OVARIAN TERATOMA IN THE MOUSE.

OVARIAN TERATOMA IN THE MOUSE.


THE POTENCY OF 20-METHYLCHOLANTHRENE RELATIVE TO OTHER  CARCINOGENS ON BLADDER IMPLANTATION.


ATTEMPTS TO TRANSFER THE MAMMARY TUMOUR AGENT FROM INFECTED MALE MICE TO  SUSCEPTIBLE NEW-BORN FEMALES.


EFFECTS OF WHOLE BODY IRRADIATION AND OF PARTIAL HEPATECTOMY  ON THE LIVER LESIONS INDUCED IN RATS BY A SINGLE DOSE OF RETRORSINE, A  PYRROLIZIDINE (SENECIO) ALKALOID.


TESTS FOR CARCINOGENESIS USING NEWBORN MICE: 1,2BENZANTHRACENE, 2-NAPHTHYLAMINE, 2-NAPHTHYLHYDROXYLAMINE AND ETHYL METHANE SULPHONATE.


THE EFFECT OF PHENANTHRENE ON TUMOUR INDUCTION BY 3,4-BENZOPYRENE  ADMINISTERED TO NEWLY BORN MICE.


STUDIES ON THE  CARCINOGENICITY OF TRICYCLOQUINAZOLINE: THE EFFECT OF STRUCTURAL MODIFICATION.


STUDIES ON 1,2:5,6-DIBENZANTHRACENE INDUCED MAMMARY  CARCINOGENESIS IN MICE.


FURTHER OBSERVATIONS ON  RADIOCURABILITY OF A SOLID EHRLICH AND TISSUE REACTIONS IN THE MOUSE WITH FRACTIONATED   RADIATION DOSES AND THE EFFECTS OF OXYGEN.


THE EFFECTS OF 2-(2: 6-XYLYLOXY)-ETHYLGUANIDINE ON SARCOMA CELLS AND NORMAL FIBROCYTES CULTIVATED IN VITRO.


ANTIBODY PRODUCTION IN TOLERANT RABBITS TO MOUSE  LYMPHOMA L-5178.


THE EFFECTS OF 1-(2-DIMETHYLAMINOETHYL)-2-PHENYLINDENE ON FIBROCYTES CULTIVATED  IN VITRO.


THE INFLUENCE OF WALKER 256 CARCINOSARCOMA ON THE METABOLISM OF  CORTICOSTERONE AND ANDROSTENOLONE IN RATS.


INHIBITION OF AN EHRLICH ASCITES   TUMOR IN VIVO BY PARTIALLY NEUTRALIZED SOLUTIONS OF SODIUM LACTATE.


THE EFFECT OF RAT SPLEEN CELLS ON TWO TRANSPLANTED  MOUSE TUMOURS.


EARLY STAGE IN THE METABOLISM OF AMINOAZO DYES IN THE LIVER OF RATS.


STUDIES ON CANCER ANAEMIA: ORGAN WEIGHTS,  BLOOD VALUES AND IRON METABOLISM IN NORMAL AND TUMOUR-BEARING MICE.


THE EFFECT OF AGE, SEX, STRAIN, SPECIES AND DOSE LEVEL DIFFERENCES UPON THE  METABOLISM OF 2-NAPHTHYLAMINE IN RODENTS.


INTERMEDIARY METABOLISM IN HELA AND HUMAN FOETAL LIVER CELLS.


THE ATTEMPTED IN VIVO INHIBITION OF THE ORTHO-HYDROXYLATION OF 2-NAPHTHYLAMINE.


MICROGNATHIA.

MICROGNATHIA.


MALIGNANT SACROCOCCYGEAL TERATOMA IN THE NEWBORN.


SOME ASPECTS OF TREATMENT OF TRAUMATIC DIPLOPIA.


THE TREATMENT OF PATIENTS SUFFERING FROM DIPLOPIA CAUSED BY DISPLACEMENT OF THE EYEBALL, DUE TO FRACTURE OF THE ORBITAL WALL.


KINETIC VISUAL ACUITY.

KINETIC VISUAL ACUITY.


THE TREATMENT OF MALUNITED FRACTURES OF THE NOSE WITH LATERAL DEVIATION.


NASOLABIAL FLAP REPAIR FOR ALAR MARGIN DEFECTS.


THE HISTORY OF THE CLASSIFICATION OF BURNS (DIAGNOSIS OF DEPTH).


ABERRATIONS OF THE EYE AND THEIR EFFECTS ON VISION: 1. SPHERICAL ABERRATION.


ALTERNATIVE PHYSIOLOGICAL MECHANISMS OF INFORMATION TRANSFER BETWEEN THE RETINA AND THE BRAIN IN VISUAL PERCEPTION.


THE LYMPHATIC VESSELS IN RADIODERMATITIS: A CLINICAL AND EXPERIMENTAL STUDY.


FACIAL SPASM.

FACIAL SPASM.


MYOCLONUS OF THE PALATE.

MYOCLONUS OF THE PALATE.


PSYCHO-OPTICAL MOVEMENTS OF THE EYES, I.


A METHOD OF ANTIHELIX RECONSTRUCTION.

A METHOD OF ANTIHELIX RECONSTRUCTION.


THE RADICAL FASCIECTOMY OPERATION FOR DUPUYTREN'S DISEASE: A CONDEMNATION.


BURIED SKIN-STRIP URETHRA IN A TUBE PEDICLE PHALLOPLASTY.


PENILE REFORM.

PENILE REFORM.


NEEDLEHOLDER FOR USE WITH FINE SUTURES.

NEEDLEHOLDER FOR USE WITH FINE SUTURES.


GENETICS AND MEDICINE.

GENETICS AND MEDICINE.


STEROID TREATMENT IN DISSEMINATED SCLEROSIS.


ESOPHAGEAL VARICES.

ESOPHAGEAL VARICES.


THE SPLEEN AND ANAEMIA.

THE SPLEEN AND ANAEMIA.


STUDIES OF URINARY TRACT INFECTIONS IN  INFANCY AND CHILDHOOD. I. ANTIBODY RESPONSE IN DIFFERENT TYPES OF URINARY TRACT INFECTIONS  CAUSED BY COLIFORM BACTERIA.


TRAUMATIC NASAL NEURALGIA AND ITS TREATMENT.


ABO BLOOD GROUPS AND ACUTE HEPATITIS.

ABO BLOOD GROUPS AND ACUTE HEPATITIS.


TRIAL OF A POWDERED FORMULA DIET IN TREATMENT OF OBESITY.


SECRETOR STATUS  OF STREPTOCOCCUS PYOGENES GROUP A CARIERS AND PATIENTS WITH RHEUMATIC HEART DISEASE OR  ACUTE GLOMERULONEPHRITIS.


AGRANULOCYTOSIS DURING ADMINISTRATION OF "ATROMID".


INFESTATION OF HUMAN EYELID BY A LARVAL TICK (IXODES  RICINUS L.).


VALVULAR DISEASE OF THE HEART. A CASE DEMONSTRATED AT THE   POSTGRADUATE MEDICAL SCHOOL OF LONDON.


ORAL CONTRACEPTIVES.

ORAL CONTRACEPTIVES.


ECONOMIC BENEFITS OF MEDICAL CARE.

ECONOMIC BENEFITS OF MEDICAL CARE.


HIGHLIGHTS OF A SURVEY OF HEALTH UNIT SERVICES IN CANADA.


REPORT ON A PARTICULAR PHAGE-TYPE OF STAPHYLOCOCCUS ISOLATED IN SOME HOSPITALS IN CANADA.


THE MILIEU INT'ERIEUR AND THE CELL THEORY.


THE USE OF POLYVALENT SOMATIC ANTIGEN VACCINE AS A NASAL SPRAY IN THE TREATMENT OF CARRIERS OF STAPHYLOCOCCUS AUREUS.


BITING FLY CONTROL AT CANADIAN SERVICE INSTALLATIONS.


APOPLEXY IN THE HIPPOCRATIC WRITINGS.

APOPLEXY IN THE HIPPOCRATIC WRITINGS.


NEONATAL RESPIRATORY DISTRESS: EXPERIENCE AT THE HOSPITAL FOR SICK CHILDREN, TORONTO, L960-1961. Four hundred and thirty infants selectively referred to the Newborn Unit of The Hospital for Sick Children, Toronto, with the symptom of respiratory distress, were reviewed. There were 142 cases of the "idiopathic respiratory distress syndrome" (IRDS), of which 67 were fatal. The remainder included 100 cardiac (76 deaths), 63 extra-pulmonary (28 deaths) and 109 other specific pulmonary conditions (54 deaths). Of the 109, half were due to massive aspiration. Serial observations and radiographs led to correct clinical diagnosis in 85% of necropsy-proved cases of IRDS and in a comparable proportion of all other conditions. The incidence and mortality rate of IRDS were twice as high in males as in females. A significant number of premature infants have transient respiratory distress after birth, and diagnostic criteria for inclusion in any study should be defined in detail. This study emphasized the large contribution of disorders of the respiratory and cardiac systems to neonatal mortality and led to the formation of a special department for intensive care and research in these conditions.


A DISINFECTED LETTER FROM BALTIMORE IN 1797.


DR. JOHN TENNENT AND THE UNIVERSAL SPECIFIC.


MESENTERIC ARTERY INSUFFICIENCY.

MESENTERIC ARTERY INSUFFICIENCY. The syndromes of superior mesenteric artery insufficiency are briefly reviewed. Three cases associated with infarction of bowel which were treated with restoration of arterial flow and resection of residual irretrievable bowel are reported. In two patients an embolectomy and in one patient a bypass graft were used to restore arterial continuity. The importance of the recognition and removal of irretrievable bowel at the time of vascular reconstruction is emphasized. Success is not necessarily predicated by the time factor alone, although the importance of early diagnosis and surgical intervention cannot be denied.


PULMONARY ASPERGILLOSIS.

PULMONARY ASPERGILLOSIS. Aspergilli are widely distributed in nature and there is abundant opportunity to acquire infection. The fungus is usually only locally invasive in healthy tissues. Its culture from sputum on one occasion may have no significance. Its growth in the bronchial tree of asthmatics may produce sensitization with aggravation of the asthma and bronchial plugging. Multiplication in dead or damaged lung tissue such as infarcts is not uncommon. The fungus may also colonize pre-existing lung cavities, especially those remaining after treatment of tuberculosis. The ball of fungus produces a characteristic radiological picture, particularly on tomograms, which is usually sufficient to make the diagnosis. Hemoptysis is common. Inaction may be preferable to excision, especially in a patient with impaired lung function. Seven cases have been described to illustrate some of the features and natural history of the condition. The mounting number of sterile cavities left in lungs has increased the number of mycetomas which have developed.


MAIMONIDES' INTERPRETATION OF THE FIRST APHORISM  OF HIPPOCRATES.


HONOURED FATHER: THE MEDICAL LETTERS OF DR. PETER BRYANT   (1767-1820).


EMOTIONAL REACTIONS TO DISFIGUREMENT FROM CANCER THERAPY. Disfigurement not only produces current anxieties but reactivates childhood conflicts. The emotional reaction depends upon the disturbance to the patient's major adaptations to life as well as the meaning of the organ to the patient. Fear of isolation and rejection by others may be more terrifying than fear of death. Emotional reactions include regression with marked dependency, anxiety, depression, hostility and, if severe, paranoid states, hypochondriasis, denial, counterphobic behaviour, obsessive-compulsive reactions and schizophrenic reactions. Management basically involves early establishment of a positive doctor-patient relationship. In such a relationship the physican should educate his patient, undercut guilt, accept transient regression and expression of anger, set limits on counterphobic behaviour, either support or gently question denial of reality, and support, without being overly sympathetic, a depressed patient. The nurse, social worker, psychiatrist and the patient's family may be valuable members of the therapy team. Disfigurements of various body areas pose individual problems of management.


DROWNING: ITS MECHANISM AND TREATMENT.

DROWNING: ITS MECHANISM AND TREATMENT. Pertinent experimental work and literature relative to drowning are reviewed. The different concepts of the mechanism of drowning are dealt with and the view is emphasized that asphyxia is complicated by hemodilution in fresh water and by hemo-concentration in salt water, with resulting electrolyte imbalance. A short description is given of the sequence of events in drowning. Lethal heart failure has occurred as early as two minutes after total submersion. Treatment consists of artificial respiration, cardiac massage, correction of the electrolyte imbalance and continued observation for complications. The danger from hyperventilation in underwater swimming, of cases of so-called "secondary" drowning and the use of diatoms as a proof of drowning are mentioned.


A NEW APPROACH TO BACTERIAL VACCINES.

A NEW APPROACH TO BACTERIAL VACCINES. Immunizing antigens against only 10 bacterial diseases-cholera, diphtheria, paratyphoid, pertussis, plague, scarlet fever, staphylococcal disease, tetanus, tuberculosis and typhoid-have been licensed for sale in Canada and the United States. Convincing evidence of efficacy is available for only four of these-diphtheria and tetanus toxoids, and pertussis and typhoid vaccines.The principles which determine the efficacy of different immunizing antigens are not always the same. Toxoids, for example, stimulate the formation of antitoxin-producing mechanisms which can neutralize toxins produced by invading organisms, thereby rendering them harmless. Conversely, vaccines stimulate the formation of antibacterial mechanisms which stop the growth of organisms before they can produce disease.Use of enzyme-lysed vaccines for prevention of staphylococcal disease represents a new approach in vaccine research. Animal tests have shown lysed vaccines to be 10 to 100 times less toxic, and about eight times more effective, than whole bacterial vaccines. Studies with lysed vaccines for other diseases are now in progress.


DE ARTHRITIDE SYMPTOMATICA OF WILLIAM MUSGRAVE  (1657-1721): HIS DESCRIPTION OF NEUROPATHIC ARTHRITIS.


BENJAMIN RUSH, INTEMPERATE DRINKING, AND THE COMMON  COUNCIL OF THE CITY OF NEW YORK.


HYPERTENSION FOLLOWING CLOSURE OF A POSTNEPHRECTOMY ARTERIOVENOUS FISTULA: A CASE REPORT AND HYPOTHESIS.


MALIGNANT PHEOCHROMOCYTOMA: REPORT OF TWO CASES.


SIMPLIFIED INDUCTION OF LABOUR FOR THE PATIENT WITH A DEAD BABY IN UTERO. The problem posed by the presence of a dead fetus in utero after the first trimester is important because of the psychological attitude of the patient and the danger of hypofibrinogenemia. In the past, induction of labour in these patients has often been difficult, and surgery has had to be undertaken to empty the uterus.In these patients, labour was induced by inserting a needle with a trocar through the abdominal wall under local anesthesia into the amniotic sac. Approximately 200 ml. of amniotic fluid is withdrawn and replaced with a slightly larger amount of 20% saline solution. Labour began within 24 hours and delivery occurred within the next 14 hours. There have been no complications or failures with this technique in the cases reported in the literature.


GENETICS OF CHROMOSOME 21.

GENETICS OF CHROMOSOME 21.


CURRENT VIEWS ON RESPIRATORY DISEASE. II.


DEATH IN THE AIR.

DEATH IN THE AIR.


SKELETAL FIXATION FOR THE CROSS-LEG PEDICLE GRAFT.


ATRIAL SEPTAL DEFECT IN INFANCY.

ATRIAL SEPTAL DEFECT IN INFANCY. The case histories of seven infants with atrial septal defect are presented to draw attention to certain features and possible dangers of this defect in infancy. Four infants were asymptomatic but one failed to thrive and two died suddenly. Five had ejection murmurs and two, with pulmonary hypertension, had loud pan-systolic murmurs with a thrill. In two infants murmurs were noted at birth, but in five they were first heard between the ages of 1 and 6 months. The second pulmonary sound was initially narrowly split in all, but became widely split between the ages of 12 and 20 months. Electrocardiograms and chest roentgenograms were of little help at the outset but later showed findings characteristic of the defect after one year. All infants were catheterized; a left-to-right atrial shunt was detected in each instance. Pulmonary hypertension was present in two infants, one of whom died.


PLACENTA ACCRETA: REPORT OF FIVE CASES AND A PLAN OF MANAGEMENT. Five cases of placenta accreta are reviewed. The diagnosis was made on clinical grounds by intrauterine examination. Two of the five patients were managed conservatively, thus preserving the uterus. The conservative approach entails leaving the pathologically involved portion of accreta placenta in situ. The only indications for hysterectomy are hemorrhage and infection. Conservative management is justified by the successful outcome and lack of puerperal morbidity noted in this study and reported by others.


RECTICULUM CELL SARCOMA OF THE CENTRAL NERVOUS SYSTEM. One variety of sarcoma of the central nervous system (CNS) has been referred to under such synonyms as perithelial sarcoma, microglioma, and reticulum cell sarcoma. A case of diffuse involvement of the CNS is reported and the author believes that the tumour is a reticulum cell sarcoma because: (1) in addition to involvement of the CNS, lesions characteristic of a diffuse lymphomatous process were present in extraneural sites; (2) silver carbonate impregnation of the tumour cells was demonstrated. This tinctorial property has been reported as characteristic of microgliomas but has been shown to be an unreliable criterion for the differentiation of the cells of microglial from those of reticulum cell sarcomas. It is concluded that in such cases the CNS is the initial and major focus of a multicentric malignant lymphoma which has the histologic characteristics of a reticulum cell sarcoma.


DE QUERVAIN'S DISEASE: STENOSING TENOVAGINITIS AT THE RADIAL STYLOID PROCESS. Twelve cases of stenosing tenovaginitis at the radial styloid process (de Quervain's disease), including two bilateral cases, are reviewed. These patients presented the typical symptoms of pain just proximal to the radial styloid process accompanied by limitation of abduction of the thumb. Many previous reports have emphasized the frequency of aberrant tendons in this region and have implied that such anomalies may play a part in the etiology of this disorder. In our cases aberrant tendons were not found. Pronounced thickening of the tendon sheaths was present at operation and accumulations of fluid resembling ganglion formation were frequently noticed. All forms of therapy except surgical excision of the involved tendon sheaths were largely ineffective. This surgical procedure was so simple and satisfactory that it is recommended as the treatment of choice. The diagnosis of de Quervain's disease is easily missed unless the examining physician constantly keeps it in mind.


OBSTRUCTIVE CARDIOMYOPATHY. REPORT OF A CASE AND REVIEW OF THE LITERATURE.


THE SIGNIFICANCE OF SPERMATOZOA IN VICTIMS OF SEXUAL OFFENCES. Factual information for the use of the doctor called upon to give evidence in court after examining the victim of an alleged sexual attack is considered on the basis of an extensive review of the literature and of years of experience by the staff of the Attorney-General's Laboratory for Ontario. This experience emphasizes the need for immediate examination of fluid from the vaginal pool for motility of the spermatozoa and the need for a later examination for delayed bruising of the victim. Motile spermatozoa are found in the vagina for one-half to six hours, with an average time of three hours. Non-motile forms usually are found for seven to 12 hours, but exceptionally for 18 to 24 hours. Data relative to the survival of spermatozoa under various conditions are tabulated.


REMOVAL OF SEBACEOUS CYSTS. A MODIFICATION IN TECHNIQUE.


CANADA'S PSYCHIATRISTS AND THEIR HISTORY (RICHARD M. BUCKE).


CURRENT VIEWS ON RESPIRATORY DISEASE. III.


NON-PROGRESS IN ESSENTIAL HYPERTENSION.

NON-PROGRESS IN ESSENTIAL HYPERTENSION.


RADIOCARDIOGRAPHY AND PULMONARY  RADIOCIRCULOGRAPHY USING I-131 ALBUMIN IN CARDIOPATHIES.


SPATIAL ELECTROCARDIOGRAPHIC PATTERNS IN INDUCED HYPOPOTASSEMIA.


THE USE OF THE ANALOG COMPUTER IN RECORDING LINEAR VECTORCARDIOGRAMS.


SERUM CREATINE PHOSPHOKINASE AND ALPHA-HYDROXYBUTYRIC DEHYDROGENASE ACTIVITIES COMPARED WITH GOT AND LDH IN MYOCARDIAL INFARCTION.


CARL JOHN WIGGERS, M. D. (1883-1963): A MEMORIAL TRIBUTE.


CARDIAC ARRHYTHMIAS FOLLOWING CONDENSER DISCHARGES AND THEIR DEPENDENCE UPON STRENGTH OF CURRENT AND PHASE OF CARDIAC CYCLE.


RESPONSE OF THE HEART  TO INCREASED PERIPHERAL RESISTANCE.


PULSATILE PRESSURE AND FLOW THROUGH DISTENSIBLE VESSELS.


EFFECTS OF INTRA-ARTERIAL AND INTRAVENOUS EPINEPHRINE ON DISAPPEARANCE OF NAI-131 FROM CALF MUSCLE AND ON CALF BLOOD FLOW.


GASEOUS DIFFUSION FROM ALVEOLI TO PULMONARY VESSELS OF CONSIDERABLE SIZE.


GRAND ROUNDS: CRETINISM.

GRAND ROUNDS: CRETINISM.


INFLUENCE OF RESPIRATORY ACIDOSIS ON CIRCULATORY EFFECT OF EPINEPHRINE IN DOGS.


GRAND ROUNDS: DISSEMINATED SCLEROSIS.

GRAND ROUNDS: DISSEMINATED SCLEROSIS.


WILMS'S TUMOR: EXPERIENCE AT CHILDREN'S HOSPITAL 1949-1959.


SEX APPENDAGES, ARNETH FORMULA, AND THE MENSTRUAL CYCLE.


NON-RANDOM DISTRIBUTION OF CHROMOSOMES  IN METAPHASE FIGURES FROM CULTURED HUMAN LEUCOCYTES. I. THE PERIPHERAL LOCATION OF THE Y CHROMOSOME.


THE INCIDENCE OF DRUMSTICKS IN PATIENTS WITH THREE X CHROMOSOMES.


AN ABERRANT AUTOSOME (13-15) IN A HUMAN FEMALE AND HER FATHER, BOTH APPARENTLY NORMAL.


A CASE OF A MENTALLY DEFECTIVE MALE WITH XXYY SEX CHROMOSOME CONSTITUTION.


AN ADULT TRIPLOID CHICKEN (GALLUS DOMESTICUS) WITH A LEFT OVOTESTIS.


BILATERAL SPONTANEOUS PNEUMOTHORAX.

BILATERAL SPONTANEOUS PNEUMOTHORAX.


BRONCHOPULMONARY ASPERGILLOSIS.

BRONCHOPULMONARY ASPERGILLOSIS.


PNEUMOCONIOSIS: AN ASPECT FROM A DIFFERENT ANGLE.


CURRENT STATUS OF CUTANEOUS LEISHMANIASIS IN ITALY.


A PHONOCARDIOGRAPHIC STUDY OF PATIENTS WITH TOTAL PROSTHETIC MITRAL VALVE REPLACEMENT.


OPPORTUNISTIC GRAM-NEGATIVE ROD PULMONARY INFECTIONS.


MECHANISM OF INFECTION IN SOUTH AMERICAN BLASTOMYCOSIS.


VALUE OF FLUORESCENT TECHNIQUES FOR DETECTING MYCOBACTERIUM LEPRAE IN TISSUE SECTIONS.


PROGNOSIS AND TREATMENT OF TUBERCULOSIS AMONG CHILDREN.


AN ACCOUNT OF CHRONIC BRONCHITIS IN GREAT BRITAIN WITH A COMPARISON BETWEEN BRITISH AND AMERICAN EXPERIENCE OF THE DISEASE.


A PRACTICAL VISCOSIMETER FOR SUSPENSIONS OF BARIUM AND METHYLCELLULOSE IN BRONCHOGRAPHY.


A TEN-YEAR PLAN FOR TUBERCULOSIS ERADICATION IN JORDAN.


LICHEN SIMPLEX CHRONICUS AMONG FILIPINOS.


TWO PUZZLES IN TROPICAL DERMATOLOGY: SPOTTED LEPROSY OF LUCIO AND BRAZILIAN PEMPHIGUS.


THORACIC SURGERY IN PATIENTS OVER SEVENTY.


HEART FAILURE IN PATIENTS WITH CHRONIC PULMONARY EMPHYSEMA.


NEUROGENOUS TUMORS OF THE MEDIASTINUM: A CLINICOPATHOLOGIC STUDY BASED ON 50 CASES.


STREPTOSPORANGIUM BOVINUM SP. NOV. FROM CATTLE HOOFS.


AINHUM AND PSEUDOAINHUM. REPORT OF THREE CASES.


WHAT'S NEW AROUND THE WORLD.

WHAT'S NEW AROUND THE WORLD.


CARDIOVASCULAR RESPONSES TO HYPERTHERMIA.


PREMATURE SYSTOLES IN HYPOPOTASSEMIA OF UNUSUAL GENESIS.


ROENTGENOGRAM OF THE MONTH.

ROENTGENOGRAM OF THE MONTH.


HYPONATREMIA, ANTIDIURETIC HORMONE SECRETION AND OAT CELL CARCINOMA OF THE LUNG.


EFFECT OF RESERPINE ON TRICHINELLA SPIRALIS INFECTION IN RATS.


PARASITE MIGRATION AND HOST REACTION IN MICE EXPOSED TO IRRADIATED CERCARIAE OF SCHISTOSOMA MANSONI.


ELECTROCARDIOGRAPHIC CHANGES OCCURRING IN TRICHINELLA-  INFECTED RATS PRIOR TO LARVIPOSITION AND MIGRATION.


DOCTORS WHO NEVER PRACTICE.

DOCTORS WHO NEVER PRACTICE.


OBSTACLES TO UNIVERSAL RECIPROCITY AND ENDORSEMENT.


CURRENT ATTITUDES TOWARD BASIC SCIENCE LAWS.


VARIABLES INFLUENCING OPERATOR INFORMATION PROCESSING.


DEVELOPMENT OF A PERIPHERAL VISION COMMAND INDICATOR FOR INSTRUMENT FLIGHT.


DYNAMIC VISION--THE LEGIBILITY OF EQUALLY SPACED ALPHANUMERIC SYMBOLS.


THE EFFECT OF RATE AND DIRECTION OF DISPLAY MOVEMENT UPON VISUAL SEARCH.


THE SELF MARKER CONCEPT AS APPLIED TO THE RH AND ABO BLOOD GROUPS.


MONITORING OF COMPLEX VISUAL DISPLAYS. IV. TRAINING FOR VIGILANCE.


OPERATOR LOADING TASKS.

OPERATOR LOADING TASKS.


SEPTIC ABORTION--HARPER HOSPITAL EXPERIENCE 1954-1960.


PERIPHERAL AUSCULTATION: PROSPECTING WITH A STETHOSCOPE.


AN EDITOR LOOKS AT DOCTORS.

AN EDITOR LOOKS AT DOCTORS.


THE BLOOD SUPPLY OF BONE.

THE BLOOD SUPPLY OF BONE.


THE ANTIBACTERIAL ACTION OF VERNIX CASEOSA.


UNUSUAL CAUSES OF INTRACTABLE CONGESTIVE CARDIAC FAILURE.


POSTOPERATIVE CARE AFTER CARDIAC SURGERY.


ANTIBIOTIC THERAPY OF BACTERIAL ENDOCARDITIS.


PREFERENCE SCALING VIA A MODIFICATION OF SHEPARD'S PROXIMITY ANALYSIS METHOD.


IDENTIFICATION OF A NEW STREPTOCOCCAL RED CELL SENSITIZING ANTIGEN.


SPECIFIC INHIBITION OF ANTIBODY PRODUCTION. III. APPARENT CHANGES IN THE HALF-LIFE OF BOVINE GAMMA GLOBULIN IN PARALYSED MICE.


A SEROLOGICAL ANALYSIS AND COMPARISON OF VARIOUS PREPARATIONS OF MOUSE H-2 HISTOCOMPATIBILITY ANTIGEN.


IMMUNOLOGICAL RELATION OF BASEMENT MEMBRANE AND A SERUM BETA GLOBULIN IN THE MOUSE. DEMONSTRATION OF RENAL BASEMENT MEMBRANE ALTERATION IN MICE INJECTED WITH STREPTOLYSIN S.


IDENTIFICATION OF THE COMPONENTS OF COMPLEMENT PARTICIPATING IN THE ANTIGLOBULIN REACTION.


IMMUNOLOGICAL UNRESPONSIVENESS TO PROTEIN ANTIGENS IN RABBITS EXPOSED TO X-IRRADIATION OR 6-MERCAPTOPURINE TREATMENT.


RADIOGRAPHIC ANATOMY OF THE CANINE HEART. II.


INDUCTION OF AUTOIMMUNITY TO ADRENAL GLAND.


OSTIUM SECUNDUM TYPE INTERATRIAL SEPTAL DEFECTS IN THE DOG.


DIOCTOPHYMA RENALE INFECTION IN A DOG.

DIOCTOPHYMA RENALE INFECTION IN A DOG.


A PLASTIC ADHESIVE FOR NONSUTURE REPAIR OF TRACHEAL WOUNDS.


THE VIRGINIA PREDATOR RABIES CONTROL PROGRAM--1961-1962.


CLINICO-PATHOLOGIC CONFERENCE.

CLINICO-PATHOLOGIC CONFERENCE.


TREATMENT OF GENITAL TRICHOMONIASIS IN BULLS.


INVESTIGATION OF JOHNE'S DISEASE IN THREE CATTLE HERDS.


AN INHALATION ANESTHETIC APPARATUS FOR LARGE ANIMALS.


ASSOCIATION OF PLEUROPNEUMONIA-LIKE ORGANISMS WITH POLYSEROSITIS IN ADULT SWINE.


RADIATION THERAPY FOR LAMENESSES IN HORSES.


"CLOSED--JOINT" REPAIR OF ANTERIOR CRUCIATE LIGAMENT RUPTURE IN THE DOG.


NUTRITIONAL MYODEGENERATION IN A LITTER OF BEAGLES.


LOW-GRADE OTOCEPHALY IN A DOG.

LOW-GRADE OTOCEPHALY IN A DOG.


TREATMENT OF DOGS WITH EXPERIMENTALLY INDUCED ACHYLIA PANCREATICA.


RECONSTRUCTION OF THE CANINE EAR CANAL WITH POLYTETRAFLUOROETHYLENE IMPLANTS.


ELECTRON MICROSCOPY OF PLASMOLYSIS IN ESCHERICHIA COLI. Cota-Robles, Eugene H. (University of California, Riverside). Electron microscopy of plasmolysis in Escherichia coli. J. Bacteriol. 85:499-503. 1963.-Escherichia coli cells plasmolyzed in 0.35 m sucrose reveal plasmolysis at one tip of a cell or in the center of dividing cells in which protoplast partition has been complete. Central plasmolysis reveals that protoplast separation can be completed before the invagination of the cell wall is complete. These studies support the concept that these cells divide by constriction. The strength of the union between cell wall and cytoplasm is not uniform around the entire cell. It is strongest along the sides of these rod-shaped cells and weakest at one tip of the single cell. Thus, a single cell generally forms one cup-shaped vacuole in which the cytoplasm has collapsed away from one tip of the cell.


IMMUNOLOGY OF THE YEAST HANSENULA WINGEI. Brookbank, John W. (University of Florida, Gainesville) and Mary R. Heisler. Immunology of the yeast Hansenula wingei. J. Bacteriol. 85:509-515. 1963.-Antisera produced in three groups of rabbits (18 animals in all) against mating types of Hansenula wingei, using three different modes of injection, failed to show specificity for mating type. However, the antisera were reactive with material obtained from the cells (presumably from the cell wall) by extraction at 100 C. A constituent of this fraction of boiled cells is in some way involved in the auto-agglutination of unboiled cells upon the disruption of these cells in a Mickle disintegrator. The antisera are of broad specificity regarding their ability to agglutinate cells of other species of Hansenula, and have been shown to react with a minimum of one antigen found in supernatants of both H. anomala and H. saturnus boiled cells in double agar diffusion tests. H. wingei supernatants (boiled cells), in reaction with homologous antisera, show additional components not shown by H. anomala or H. saturnus.


RAPID QUANTITATIVE METHOD FOR MEASURING THE SENSITIVITY OF BLASTOMYCES DERMATITIDIS TO FUNGISTATIC AGENTS. Guidry, D. J. (Louisiana State University School of Medicine, New Orleans) and Findlay Maier. Rapid quantitative method for measuring the sensitivity of Blastomyces dermatitidis to fungistatic agents. J. Bacteriol. 85:504-508. 1963.-Isolates of Blastomyces dermatitidis (yeast phase) were grown in continuous shake liquid culture. When sufficient cells were added to impart an initial optical density of 0.100 to the inoculated medium, growth was rapid, and the time required to assay fungistatic agents was reduced to 48 hr. Standard curves on light transmittance and direct cell count facilitated preparation of inoculum and measurement of growth. Generation time was determined for various growth phases of B. dermatitidis, and the data were applied to selection of the optimal period of growth for assaying fungistatic agents. Four isolates of B. dermatitidis were tested for their sensitivity to amphotericin and 2-hydroxystilbamidine. An amphotericin B concentration of 1.4 mug/ml was sufficient for maximal inhibition of all isolates tested; there were differences among isolates in sensitivity to lower concentrations of the drug. A 2-hydroxystilbamidine concentration of 62.5 mug/ml was sufficient for maximal inhibition of two isolates; the two remaining isolates required a concentration of 125 mug/ml. A latent period in inhibition of growth occurred when amphotericin B and 2-hydroxystilbamidine were added to cultures of B. dermatitidis. This latent period, together with generation time of the yeast cells, determined the maximal degree of inhibition which could be attained.


EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. I. NATURE OF THE SUBSTRATE AND ENZYME INVOLVED IN THE EGG YOLK OPACITY REACTION. Shah, D. B. (University of Wisconsin, Madison) and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. I. Nature of the substrate and enzyme involved in the egg yolk opacity reaction. J. Bacteriol. 85:516-521. 1963.-Some pathogenic staphylococci produce an opacity reaction when grown in media containing egg yolk. The egg yolk factor is a lipase with a requirement for a fatty acid acceptor, rather than any of the three lecithinases. Highest amounts of egg yolk factor were obtained in Heart Infusion broth rather than nutrient broth or Casman's synthetic medium. The lowest density lipo-protein (lipovitellenin) isolated by ultracentrifugation of egg yolk has been identified as the opacity-producing substrate. The lipase acts on the lipid moiety, resulting in alterations in the solubility of lipovitellenin. Lipolysis is optimal at pH 8. The optimal pH for the opacity reaction is at pH 5.5 and 8. The protein (vitellenin) in lipovitellenin has an isoelectric point at pH 5.5. It is proposed that slight alterations in the stabilizing lipid due to lipolysis and the very low solubility of vitellenin may drastically alter the solubility of lipovitellenin at pH 5.5.


INTRACELLULAR HEMOLYSIN OF PSEUDOMONAS AERUGINOSA. Berk, Richard S. (Wayne State University, Detroit, Mich.). Intracellular hemolysin of Pseudomonas aeruginosa. J. Bacteriol. 85:522-526. 1963.-Disruption of cells (2 to 6 days old) of Pseudomonas aeruginosa grown in Tryptose Broth devoid of blood yielded an intracellular hemolysin active on sheep and human erythrocytes. The intracellular agent was not usually detectable in cells grown under highly aerobic conditions. However, activity was observed after 48 hr of growth and appeared to reach a peak at 96 hr when grown under static conditions. Centrifugation of hemolytic extracts at 20,000 x g for 30 min resulted in a fractionation of activity into particulate and soluble components. Similar fractionation of activity occurred after adjustment of the pH of crude extracts to 1.40. In addition, hemolytic preparations were not inactivated by boiling for 90 min, by treatment with ethylenediaminetetraacetate, or by heating in equal volumes of 20% KOH. Activity over a wide pH range was noted, with a maximum occurring at approximately 6.0. However, some preparations occasionally exhibited a second peak on the alkaline side.


LETHALITY FOR MICE OF VEGETATIVE AND SPORE FORMS OF BACILLUS CEREUS AND BACILLUS CEREUS-LIKE INSECT PATHOGENS INJECTED INTRAPERITONEALLY AND SUBCUTANEOUSLY. Lamanna, Carl (Army Research Office, Washington, D.C.) and Linda Jones. Lethality for mice of vegetative and spore forms of Bacillus cereus and Bacillus cereus-like insect pathogens injected intraperitoneally and subcutaneously. J. Bacteriol. 85:532-535. 1963.-Lethality for mice of Bacillus thuringiensis var. thuringiensis (two strains), var. sotto (one strain), var. alesti (one strain), and B. entomocidus var. entomocidus (one strain) maintained on artificial laboratory media is dependent upon the route of injection, the number of the bacilli, and their biological state, i.e., vegetative or spore form. Deaths did not occur upon subcutaneous injection of tenfold greater numbers of organisms than the ld(50) dose for the intraperitoneal route. From 10 to 100 times the number of spores were required to kill than vegetative bacilli given intraperitoneally. These observations duplicated those made with strains of B. cereus. For both types of spore forms, from 5 x 10(5) to 5 x 10(7) vegetative organisms were required for one intraperitoneal ld(50). With two strains of highly virulent B. anthracis, ld(50) values were the same for the vegetative and spore states, and for subcutaneous and intraperitoneal injections. Thus, the insect pathogenic sporeforming bacteria tested are more like B. cereus than like B. anthracis in their capacity to infect mice. The similarity to B. cereus extends to the rapidity of death after fatal injection of the insect pathogenic strains. The medical and taxonomic implications of the observations are discussed.


EFFECT OF ANTIBIOTICS AND INHIBITORS ON M PROTEIN SYNTHESIS. Brock, Thomas D. (Western Reserve University, Cleveland, Ohio). Effect of antibiotics and inhibitors on M protein synthesis. J. Bacteriol. 85:527-531. 1963.-This work extends the observations of Fox and Krampitz on M protein synthesis in nongrowing cells of streptococci. A survey of a large number of antibiotics and other potential inhibitors was made. Some substances bring about inhibition of fermentation and inhibit M protein synthesis because they deprive the cell of the energy needed for this process. A second group of substances inhibit growth at concentrations tenfold or more lower than they inhibit M protein synthesis. These are the antibiotics which inhibit synthesis of cell wall or other structures in growing cells, but do not affect protein synthesis. A third group of substances inhibit growth and M protein synthesis at the same concentration. These substances probably inhibit growth because they inhibit general protein synthesis, and are therefore specific inhibitors of protein synthesis. In this class are chloramphenicol, erythromycin, and the tetracyclines. Several other antibiotics of previously unknown mode of action are in this class. A fourth group of substances had no effect on M protein synthesis. No substances were found which inhibited M protein synthesis at a lower concentration than that which inhibited growth. M protein synthesis in nongrowing cells may be a useful model system for obtaining a detailed understanding of protein synthesis.


INTRACELLULAR M PROTEIN OF GROUP A STREPTOCOCCUS. Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasmic membrane. When trypsinized whole cells (from which the M protein on the cell wall had been digested) or protoplasts were extracted with hot HCl, no M protein antigen was detected, although the antigen was routinely extracted from the cell walls of normal streptococci by the hot acid procedure. Various serological techniques, including the use of fluorescein-labeled type-specific antisera, were employed to demonstrate the M antigen in association with the membrane from osmotically lysed protoplasts.


SEROLOGICAL STUDIES OF O ANTIGENS OF ESCHERICHIA COLI BY MEANS OF THE HEMAGGLUTINATION TEST. Kunin, Calvin M. (University of Virginia, Charlottesville) and Mary V. Beard. Serological studies of O antigens of Escherichia coli by means of the hemagglutination test. J. Bacteriol. 85:541-548. 1963.-The serological interrelationships among most of the known O antigens of Escherichia coli were studied by the hemagglutination test. Good agreement with the bacterial agglutination test was found with the major antigens, but cross reactions among groups were much more limited with the hemagglutination test. Heterogenetic cross reactions with many Enterobacteriaceae species were observed with rabbit antisera to E. coli O14, O56, O124, and O144. This effect tended to elevate antibody titers observed in human serum against almost all O antigen groups, but could not by itself account for the wide diversity of antibodies to them. E. coli antibodies were localized in human gamma-globulin, but were present in the beta-globulin fractions of a number of domestic animals. Rabbits differed from other animals studied in that antibodies to E. coli were rarely found in their sera; this was attributed in part to the low density of E. coli populations in this animal. The hemagglutination test may be used in identification of unknown O antigens, but does not have special merit over the bacterial agglutination test; it does not further characterize rough or untypable strains other than by demonstrating that they also possess heterogenetic antigens.


IMMUNOLOGICALLY ACTIVE POLYSACCHARIDES FROM NOCARDIA ASTEROIDES AND NOCARDIA BRASILIENSIS. Zamora, A. (University of Mexico, Mexico, D.F.), L. F. Bojalil, and Fernando Bastarrachea. Immunologically active polysaccharides from Nocardia asteroides and Nocardia brasiliensis. J. Bacteriol. 85:549-555. 1963.-Two immunologically active polysaccharides were isolated from Nocardia asteroides (Poly I Na and Poly II Na) and N. brasiliensis (Poly I Nb and Poly II Nb). These polysaccharides were isolated from cell extracts and purified by methanol precipitation, chloroform extraction of extraneous material, and deproteinization with trichloroacetic acid. The crucial step used for separation of Poly I and Poly II from both nocardias was differential solubility. From dried preparations containing both polysaccharides, Poly I was solubilized at pH 10, whereas Poly II remained insoluble and was subsequently solubilized at pH 5. Poly I Na and Poly I Nb are apparently the same. Arabinose and galactose were the monosaccharide constituents of these polysaccharides, and their molar ratios were similar. Furthermore, Poly I Na and Poly I Nb cross-reacted in agar diffusion precipitin tests with rabbit antisera prepared against either N. asteroides or N. brasiliensis. Either polysaccharide absorbed serum antibodies against the other. These polysaccharides can be regarded as groupspecific. Poly II Na and Poly II Nb are different and species-specific. They are composed of arabinose, galactose, and mannose but exhibit different molar ratios of these sugars according to species. They reacted only with homologous antisera.


PRODUCTION OF AN INTERFERON BY L CELLS INFECTED WITH WESTERN EQUINE ENCEPHALOMYELITIS VIRUS. Lockart, Royce Z., Jr. (The University of Texas, Austin). Production of an interferon by L cells infected with Western equine encephalomyelitis virus. J. Bacteriol. 85:556-566. 1963.-Two strains of Western equine encephalomyelitis virus (WEE), WEE (L+) and WEE (L-), which differed with respect to their cytopathogenicity for L cells were isolated. Both strains reproduced in L cells, and both induced the production of an interferon distinct from virus particles. L-cell monolayers were protected from degeneration by prior addition of interferon. By use of the absence of cytopathic effects (CPE) as an end point, interferon content was assayed. Monolayers failing to show CPE consistently produced less than 2% as much virus as control monolayers, indicating that virus synthesis was also inhibited. The use of this assay method was facilitated by the use of horse serum that appeared to contain antibodies against WEE and that permitted interferon to act selectively in the presence of active virus. It was found that interferon was produced during the time in which active virus was produced, and not significantly later. No interferon could be found in fluids from cells treated with inactive virus, although these are known to act as interfering agents. Interferon production was inhibited by pretreatment of L cells with sufficient amounts of interferon. It is concluded that interferon production is closely connected with WEE virus synthesis in L cells. The question is raised as to whether interferon need be a necessary intermediate for interference in L cells.


INORGANIC POLYPHOSPHATE METABOLISM IN CHLOROBIUM THIOSULFATOPHILUM. Hughes, D. E. (Dartmouth Medical School, Hanover, N.H.), S. F. Conti, and R. C. Fuller. Inorganic polyphosphate metabolism in Chlorobium thiosulfatophilum. J. Bacteriol. 85:577-584. 1963.-Cells of the obligate phototroph Chlorobium thiosulfatophilum, when grown on a normal concentration of inorganic phosphate, accumulated large intracellular metachromatic granules identified as polymetaphosphate. Inorganic phosphate was released from polymetaphosphate by cell-free extracts. This release was adenosine diphosphate-dependent and light-independent. When cells were subcultured through serial transfer in the absence of inorganic phosphate, the production of polymetaphosphate granules was almost completely stopped. After the serial transfer, these cells showed little or no endogenous release of inorganic phosphate or release from added polymetaphosphate. Using such polymetaphosphate-free cells, it has been possible to demonstrate photosynthetic phosphorylation by the naturally occurring photosynthetic macro-molecules isolated from this organism. These particles, of about 100 A in diameter with a molecular weight of approximately 1.5 million, are by far the simplest functional naturally occurring photosynthetic electron-transport units thus far described.


IDENTIFICATION OF STIMULATORY FACTOR INVOLVED IN SYMBIOTIC GROWTH OF STREPTOCOCCUS LACTIS AND STREPTOCOCCUS CREMORIS. Dahiya, R. S. (North Carolina State College, Raleigh) and M. L. Speck. Identification of stimulatory factor involved in symbiotic growth of Streptococcus lactis and Streptococcus cremoris. J. Bacteriol. 85:585-589. 1963.-Single-strain cultures of Streptococcus lactis and S. cremoris isolated from a commercial starter culture showed symbiotic growth in milk. This study dealt with identification of the main component responsible for high acid production resulting from the combined growth of these cultures. Paper chromatography and bioautography were adapted to isolate the main stimulatory factor excreted by the slower-growing culture (S. lactis) in the culture media. It was identified as adenine on the basis of ultraviolet absorption spectra and paper chromatographic R(F) value. Pure adenine possessed the same properties and was also stimulatory to the faster-growing culture (S. cremoris) when added to milk.


ACTION OF DIHYDROSTREPTOMYCIN AND ANTAGONISM BY CATIONS. Bragg, P. D. (University of British Columbia, Vancouver, Canada) and W. J. Polglase. Action of dihydrostreptomycin and antagonism by cations. J. Bacteriol. 85:590-594. 1963.-A number of antibiotics including dihydrostreptomycin inhibited the induction of beta-galactosidase in Escherichia coli and, except in the case of chloramphenicol, magnesium antagonized the inhibition. Of the antibiotics tested, only dihydrostreptomycin caused formation of pyruvate from the oxidation of glucose. Under similar conditions, inhibitors of terminal respiration (cyanide, azide, amobarbital) also caused formation of pyruvate. Magnesium and the polyamines, putrescine and spermidine, were observed to antagonize dihydrostreptomycin in systems in which the antibiotic showed an inhibitory action.


ISOLATION AND PROPERTIES OF AN IRON-OXIDIZING THIOBACILLUS. Razzell, W. E. (British Columbia Research Council, Vancouver, Canada) and P. C. Trussell. Isolation and properties of an iron-oxidizing Thiobacillus. J. Bacteriol. 85:595-603. 1963. - An organism isolated from acidic copper-leaching waters has been shown to oxidize ferrous ions, sulfur, and metallic sulfides but exhibit peculiar responses to thiosulfate. The name Thiobacillus ferrooxidans has been used to describe it. A pH of 2.5 is optimal for growth on iron, sulfur, and metallic sulfides, but cells free from iron can be obtained from growth at pH 1.6, and sulfur cultures adjusted to pH 5.5 readily attain a pH of 1.8. A stationary cultivation procedure appears superior to percolation techniques for studying the oxidation of finely divided metallic sulfides. Concentrations of soluble copper in excess of 1 g per liter were obtained from chalcopyrite in less than 4 weeks. Chalcocite oxidation proceeded in the absence of iron. Sodium chloride inhibits iron oxidation without preventing oxidation of metallic sulfides by the organism.


MICROBIOLOGICAL DISSIMILATION OF TRICARBALLYLATE AND TRANS-ACONITATE. Altekar, W. W. (National Chemical Laboratory, Poona, India) and M. R. Raghavendra Rao. Microbiological dissimilation of tricarballylate and trans-aconitate. J. Bacteriol. 85:604-613. 1963.-Two fluorescent pseudomonads capable of metabolizing tricarballylate and trans-aconitate were isolated by the soil-enrichment culture technique. These and some other species of bacteria were tested for their ability to utilize for growth the salts of many di- and tricarboxylic acids. Alloisocitrate and mesaconate were not utilized by any of the ten strains tested; only two strains grew on tricarballylate and itaconate. trans-Aconitate was utilized by many strains which had not been previously exposed to this compound. The resting cells of two strains could adapt to oxidize two acids (tricarballylate and trans-aconitate), and this induction was chloramphenicol-sensitive. The tricarballylate-grown cells were simultaneously induced to oxidize trans-aconitate and other tricarboxylates, whereas the trans-aconitate-grown cells were not induced to oxidize tricarballylate, and their subsequent induction was inhibited by chloramphenicol. This trans-aconitate or tricarballylate. But tricarballylate dehydrogenase was present only in tricarballylate-grown cells. The cell-free extracts of the two organisms contained the enzymes of the Krebs cycle and isocitritase. These enzymes are most probably operative during growth on and oxidation of these two acids as sole carbon sources.


RESPIRATORY INHIBITOR FROM SERRATIA MARCESCENS. Neufeld, Harold A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Ronald L. Coleman, and Joseph S. Zannucci. Respiratory inhibitor from Serratia marcescens. J. Bacteriol. 85:614-618. 1963.-A substance was obtained from Serratia marcescens in conjunction with lipopolysaccharide that is a potent inhibitor of mammalian succinoxidase activity. It was determined that the purified lipopolysaccharide is not an in vitro inhibitor of the succinoxidase system. The inhibitor has no effect on succinic dehydrogenase or cytochrome oxidase but apparently exerts its effect on some portion of the electron-transport chain between the dehydrogenase and cytochrome c.


GROWTH OF L FORMS OF STREPTOMYCIN-DEPENDENT PROTEUS MIRABILIS. Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also present. The 3A-type L form grows at maximal rate when the concentration of dihydrostreptomycin is between 10 and 50 mug/ml, whereas the 3B-type L form grows appreciably only at concentrations of dihydrostreptomycin of 500 mug/ml or more. These results strongly suggest that propagation of 3A-type L forms does not proceed through intermediate formation of a 3B-type L body. The low quantitative dihydrostreptomycin requirement for growth of the 3A-type L forms indicates an abnormal membrane as compared with the 3B-type L forms or the normal cells of this streptomycin-dependent mutant.


GROWTH OF CELLULAR FORMS IN CULTURES OF CHROMATIN BODIES ISOLATED FROM BACILLUS MEGATERIUM. Chatterjee, B. R. (Baylor University College of Medicine, Houston, Texas) and Robert P. Williams. Growth of cellular forms in cultures of chromatin bodies isolated from Bacillus megaterium. J. Bacteriol. 85:623-627. 1963.-Chromatin bodies isolated from old cultures of Bacillus megaterium were capable of growing into protoplastlike cells when cultured in broth enriched with horse serum, yeast extract, adenosine triphosphate, and penicillin. A tendency toward formation of rod forms of bacteria was observed in such cultures. Omission of penicillin from the medium resulted in development of short bacterial forms. In 3 of 29 experiments, actual bacillary forms indistinguishable from the parent B. megaterium organism were recovered. Culture of the chromatin bodies in plain nutrient broth did not produce any growth. Inoculation on serum-enriched agar medium of a culture of chromatin bodies, after they had begun multiplication in serum-enriched broth, resulted in development of large bodies characteristic of L forms. Ability of chromatin bodies to grow was not affected by heating for 2 hr at 80 C or by sonic treatment for up to 25 min. The possible role of such resistant chromatin bodies in the latency and persistence of infectious diseases was discussed.


FIBRINOGEN CLOTTING AND FIBRINO-PEPTIDE FORMATION BY STAPHYLOCOAGULASE AND THE COAGULASE-REACTING FACTOR. Drummond, Margaret C. (Emory University, Atlanta, Ga.) and Morris Tager. Fibrinogen clotting and fibrino-peptide formation by staphylocoagulase and the coagulase-reacting factor. J. Bacteriol. 85:628-635. 1963.-The mechanism of fibrinogen clotting by staphylocoagulase and the coagulase-reacting factor (CRF) was studied from the standpoint of the products of the reaction, and compared with thrombin clotting of fibrinogen. Both clotting principles effect clotting with the release of nonprotein nitrogenous material, which can be resolved into three peptides by Dowex-50 chromatography. On the basis of the sedimentation constants, electrophoretic mobilities, and end-group analyses of these peptides, it appears that the products of fibrinogen clotting by coagulase-CRF and by thrombin are similar.


OPTIMAL CONDITIONS FOR THE TRANSFORMATION OF STREPTOCOCCI. Perry, Dennis (Northwestern University Medical School, Chicago, Ill.) and Hutton D. Slade. Optimal conditions for the transformation of streptococci. J. Bacteriol. 85:636-642. 1963.-A study of the properties of the streptococcal transforming system, employing streptomycin resistance as a marker, resulted in a 1,000- to 10,000-fold increase in the rate of transformation. In some cases, as high as 0.5% transformants have been obtained. Certain aspects of the system differed markedly from those of other bacterial transforming systems, particularly with regard to the time for the appearance of competence. When a group H strain was exposed to deoxyribonucleic acid after various periods of incubation, the time at which cells were most competent was about 2 hr. Similar experiments with a different group H strain and an ungroupable strain showed that competence for both occurred after 1 hr of growth. It is significant that the period of optimal competence, though attained at different times, took place at the beginning of the logarithmic phase of growth. The temperature optimum for transformation was about 37 C. Cells preincubated in sheepblood broth exhibited a higher level of transformation than cells preincubated in plain or human serum broth. That some correlation exists between the attainment of competence and growth is evident. In contrast, human serum was most effective in the transforming media. The few preparations of bovine albumin (fraction V) employed did not appreciably support transformation. Data are also presented which show that some relationship exists between transforming efficiency and serological classification, in that homologous transformation showed a 100- to 10,000-fold greater efficiency over heterologous transformation. Antibodies to deoxyribonuclease, as well as other inhibitors of deoxyribonuclease, failed to bring about transformation in group A streptococci.


NORMAL FLORA OF THE NOSE, THROAT, AND LOWER INTESTINE OF DOGS. Clapper, W. E. (The Lovelace Foundation for Medical Education and Research, Albuquerque, N.M.) and G. H. Meade. Normal flora of the nose, throat, and lower intestine of dogs. J. Bacteriol. 85:643-648. 1963.-An attempt was made to isolate and identify the complete normal flora of the rectum, nose, and throat of beagles. For primary isolation, 12 different kinds of media were used. Incubation of blood agar plates and slants anaerobically, and of thioglycolate broth aerobically, allowed the growth of obligate anaerobes. From the rectal specimens, 20 species of bacteria and 10 species of fungi were isolated and identified. The organisms were similar to those found in the human intestine. Escherichia coli, Streptococcus mitis, enterococci, S. lactis, Bacillus species, and coliforms other than E. coli were most frequently encountered. The frequency of occurrence was approximately the same at both samplings in more commonly cultured bacteria. Pathogenic E. coli were isolated from nearly one-third of the first specimens. These were the only human pathogens observed. In the throat cultures, 29 species of bacteria and 2 species of yeasts were identified, and 27 species of bacteria were identified from the nasal cultures. S. mitis, Neisseria, and coagulase-negative Staphylococcus were most often isolated. The flora was similar to that found in human nose and throat cultures, except that more Haemophilus and pneumococcus and fewer coliforms are generally found in human throats. Organisms resembling human pathogens were group A streptococci and coagulase-positive staphylococci. These were isolated infrequently. It appears that this kind of examination would reveal any significant changes in normal flora that might be related to the health of the animal.


MORPHOLOGY OF HUMAN GENITAL "T-STRAIN" PLEUROPNEUMONIA-LIKE ORGANISMS. Ford, Denys K. (University of British Columbia, Vancouver, Canada), and Joyce Macdonald. Morphology of human genital "T-strain" pleuropneumonia-like organisms. J. Bacteriol. 85:649-653. 1963.-The morphology of Shepard's "T-strain" organisms from the human genital tract was investigated. The incubation of "T-strains" in 20% CO(2) with 80% nitrogen for 4 to 5 days caused surface outgrowth from the central core of the colonies embedded in the agar. Filtration through HA, PH, and VC Millipore filters showed that the elementary particles of "T-strains" were between 0.3 and 0.1 mu in diameter. "T-strain" pleuropneumonia-like organisms (PPLO) from broth cultures, stained by Giemsa's method, were seen to be minute, spherical particles, similar in size to the particles of the large-colony strains. Colonies of "T-strains," when prepared by the microculture, fixed-agar-block, and Formvar methods, resembled those of large-colony PPLO. It was concluded that "T-strain" organisms were true PPLO.


DEGRADATION OF ERGOTHIONEINE BY CELL-FREE EXTRACTS OF ALCALIGENES FAECALIS. II. PRODUCTION OF GLUTAMIC ACID. Booth, James S. (University of Southern California, Los Angeles) and Milo D. Appleman. Degradation of ergothioneine by cell-free extracts of Alcaligenes faecalis. II. Production of glutamic acid. J. Bacteriol. 85:654-657. 1963.-On the basis of oxidation and paper chromatographic procedures, glutamic acid was identified as the end product of ergothioneine degradation by cell-free extracts of Alcaligenes faecalis. Hydrogen sulfide and ammonia yields were determined. Several differences between the metabolism of whole cells and cell-free extracts were noted. Cleavage of the imidazole ring by cell-free extracts appeared to be hydrolytic rather than oxidative.


SHORT-TERM N2-15 INCORPORATION BY AZOBACTER. Bulen, W. A. (Charles F. Kettering Research Laboratory, Yellow Springs, Ohio), J. R. LeComte, and H. E. Bales. Short-term N(2) (15)-incorporation by Azotobacter. J. Bacteriol. 85:666-670. 1963.-Short-term N(2) (15)-incorporation measurements were used to determine which of the growth requirements were necessary for nitrogen fixation by Azotobacter agilis (A. vinelandii). Normal cells required neither added iron nor molybdenum, but a marked stimulation by Na(+) and a minor stimulation by Mg(2+) were observed. The Na(+) stimulation was not accompanied by an increase in O(2) uptake. A lag period preceded the response of molybdenum-deficient cells to added Mo. In systems employing 10 and 20% O(2) with 10% N(2) (15) in the gas phase, O(2) appeared to be both required and inhibitory. These observations may be helpful in attempts to fractionate cell-free nitrogen-fixing systems from this aerobe.


ULTRAVIOLET IRRADIATION OF NATIVE AND DENATURED TRANSFORMING DEOXYRIBONUCLEIC ACID FROM HAEMOPHILUS INFLUENZAE. Cabrera-Juarez, Emiliano (Johns Hopkins University School of Hygiene and Public Health, Baltimore, Md.) and Roger M. Herriott. Ultraviolet irradiation of native and denatured transforming deoxyribonucleic acid from Haemophilus influenzae. J. Bacteriol. 85:671-675. 1963.-Two antibiotic-resistance markers in denatured deoxyribonucleic acid (DNA) from Haemophilus influenzae showed about the same sensitivity to ultraviolet irradiation as in native DNA. The resistance of markers in denatured DNA to ultraviolet (UV) light increases with alkalinity up to pH 12. New transforming markers or genetic "lethals" were not produced by in vitro irradiation of either native or denatured DNA. The UV irradiation of the denatured DNA reduces its capacity to form new markers with nitrous acid. Nitrous acid reactivated some of the intrinsic marker destroyed by UV irradiation of denatured transforming DNA.


QUANTITATION OF VIRUSES BY THE PLAQUE TECNHIQUE. Berg, Gerald (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Eugene K. Harris, Shih L. Chang, and Kenneth A. Busch. Quantitation of viruses by the plaque technique. J. Bacteriol. 85:691-700. 1963.-This paper presents the results of a study on overcrowding as it occurred with several strains of enteroviruses on monkey kidney cell layers. Each cell sheet area was 4,500 mm(2). Dispersion analysis of all plaque counts in the study revealed that the percentage error, in the absence of overcrowding, fluctuated within a range of 7 to 36% about a mean value of about 17%, which is close to what is expected from purely statistical variation in sampling. Overcrowding with Mahoney virus, a mixture of particles producing either rapidly or slowly expanding plaques, resulted primarily from the obscuring effect produced as rapidly expanding plaques obliterated infected foci produced by virus particles responsible for slow-forming plaques. Overcrowding occurred at plaque levels in excess of 35 to 40 per cell sheet. Overcrowding with Mahoney LP virus, a strain derived from a rapidly expanding Mahoney plaque which produced uniformly expanding plaques, did not occur until counts in excess of 60 to 70 per cell sheet were reached. Plaque obscuring was again responsible. Apparent overcrowding with Coxsackie A9 virus, when plaques were not permanently marked from the first day of counting, resulted from coalescence at levels above about 40 to 50 plaques per cell sheet. When plaques were permanently marked, overcrowding resulted from plaque obscuring which did not occur until levels in excess of about 120 per cell sheet were reached.


STREPTOMYCES SPECIES COMPRISING THE BLUE-SPORE SERIES. Trejo, W. H. (Squibb Institute for Medical Research, New Brunswick, N.J.) and R. E. Bennett. Streptomyces species comprising the blue-spore series. J. Bacteriol. 85:676-690. 1963.-The objective of this study was to define and delimit the streptomycetes of the blue-spored (Viridochromogenes) series. The series, as defined in this study, includes 11 blue and blue-green species. The green-spored species were excluded on the basis of morphology as well as color. It was proposed that NRRL B-1511 be designated as the neotype strain of Streptomyces viridochromogenes (Krainsky) Waksman and Henrici, and that IMRU 3761 be designated as the neotype for Streptomyces cyaneus (Krassilnikov) Waksman. Evidence was presented to show that physiological criteria cannot be used to differentiate these organisms below the series level. The major characteristics of the Viridochromogenes series are blue to blue-green spores borne in spirals, and chromogenicity (melanin-positive). Reverse color and spore morphology provide a basis for separation below the series level.


DIAMINOPIMELIC ACID DECARBOXYLASE OF THE AGENT OF MENINGOPNEUMONITIS. Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse C. Tribby. Diaminopimelic acid decarboxylase of the agent of meningopneumonitis. J. Bacteriol. 85:701-706. 1963.-Evidence is presented for the presence in meningopneumonitis particles and extracts of an enzyme decarboxylating alpha, epsilon-diaminopimelic acid to lysine and for the absence of a corresponding enzyme in the uninfected host. Properties of the enzyme are described and compared with those of bacterial diaminopimelic acid decarboxylases. The significance of these observations with respect to the mode of lysine biosynthesis in the psittacosis group and to its phylogenetic origin is pointed out.


INHIBITION OF THE GROWTH OF AGENTS OF THE PSITTACOSIS GROUP BY D-CYCLOSERINE AND ITS SPECIFIC REVERSAL BY D-ALANINE. Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Julius E. Officer. Inhibition of the growth of agents of the psittacosis group by d-cycloserine and its specific reversal by d-alanine. J. Bacteriol. 85:707-711. 1963.-d-Cycloserine inhibited multiplication of four members of the psittacosis group in chick embryo yolk sac. d-Alanine reversed each inhibition. In infections with the agent of mouse pneumonitis, the most sensitive member of the psittacosis group tested, d-alanine competitively antagonized the growth inhibition produced by d-cycloserine. Of a number of other potential reversing agents, only dl-alanyl-dl-alanine reversed the effect of d-cycloserine on mouse pneumonitis agent. The significance of the susceptibility of the psittacosis group to d-cycloserine is discussed in light of the known mode of action of this antibiotic on bacteria.


MODIFIED PRESS FOR DISRUPTION OF MICROORGANISMS.


SMALL PARTICLES ASSOCIATED WITH POX VIRUSES.


EFFECTS OF TEMPERATURE ON SOIL CONIDIA OF BLASTOMYCES DERMATITIDIS.


DESIGNATION OF STAPHYLOCOCCAL EXTEROTOXINS.


MEDIUM FOR THE CONTROL OF BACTERIAL SWARMERS.


SALT--A PRESERVATIVE FOR BACTERIAL CULTURES.


DRY RUPTURE OF BACTERIAL SPORES.

DRY RUPTURE OF BACTERIAL SPORES.


ETHANOL CONVERSION IN THE BOVINE RUMEN.

ETHANOL CONVERSION IN THE BOVINE RUMEN.


DIFFERENTIAL MEDIUM FOR SELECTION AND ENUMERATION OF MEMBERS OF THE GENUS PSEUDOMONAS.


PREDICTING FULL SCALE WAIS IQS FROM WAIS SUBTESTS FOR A PSYCHIATRIC  POPULATION.


MEDICAL GENETICS 1962.

MEDICAL GENETICS 1962.


A LONGITUDINAL COMPARISON OF THE WISC AND WAIS WITH EDUCABLE MENTALLY RETARDED  NEGROES.


A NOTE ON THE SATZ-MOGEL ABBREVIATION OF THE WAIS.


THE EFFECT OF ANXIETY AS MEASURED BY THE TAYLOR MAS SCALE ON  PERFORMANCE ON THE GORHAM PROVERB TESTS.


THE MANIFEST HOSTILITY SCALE: A VALIDATION STUDY.


A PRISON MALADJUSTMENT SCALE FOR THE MMPI.


MMPI TEST TAKING TIME AND SOCIAL DESIRABILITY.


THE MF SCALE OF THE MMPI FOR COLLEGE STUDENTS.


INTEGRATING SCIENTIFIC AND PROFESSIONAL TRAINING AT THE GRADUATE LEVEL.


AN OBVERSE FACTOR ANALYTIC STUDY OF VALUES IN   PSYCHOLOGISTS, PSYCHIATRISTS, SOCIAL WORKERS AND NURSES.


THE RELATIONSHIP BETWEEN AUTHORITARIAN ATTITUDES, ATTITUDES TOWARD MENTAL  PATIENTS AND EFFECTIVENESS OF CLINICAL WORK WITH MENTAL PATIENTS.


A FURTHER STUDY ON ROTATION: CROSS-VALIDATION.


THE EFFECT OF MUSICAL TEMPO IN VARYING OPERANT  SPEECH LEVELS IN GROUP THERAPY.


THE GENERAL APTITUDE TEST BATTERY AS PREDICTOR OF VOCATIONAL READJUSTMENT BY  PSYCHIATRIC PATIENTS.


SOME MEASURES OF SELF-ACCEPTANCE.

SOME MEASURES OF SELF-ACCEPTANCE.


"STATE LEGISLATION REPORT" BROUGHT UP TO DATE.


THE BINET VOCABULARY SCORE AS AN ESTIMATE OF INTELLECTUAL FUNCTIONING.


AN EXISTENTIAL THEORY OF ANXIETY.

AN EXISTENTIAL THEORY OF ANXIETY.


THE MINNESOTA PERCEPTO-DIAGNOSTIC TEST.

THE MINNESOTA PERCEPTO-DIAGNOSTIC TEST.


AN ANALYSIS OF POLYGRAPHIC RESPONSES OF CHRONIC  REGRESSED MALE SCHIZOPHRENIC PATIENTS TO FREUDIAN-TYPE STIMULI.


SOME PERSONALITY CORRELATES OF BLOOD PROTEIN BOUND IODINE.


PERSONALITY CHARACTERISTICS WHICH DIFFERENTIATE TWO TYPES OF HEARING  DIFFICULTIES.


EGO STRENGTH AND EGO DISJUNCTION IN PRIMARY AND  SECONDARY PSYCHOPATHS.


THE RELIABILITY  AND VALIDITY OF QUANTIFIED JUDGMENTS FROM PSYCHOLOGICAL TESTS.


DIAGNOSTIC AGREEMENT OF VARIOUSLY TRAINED PSYCHOLOGISTS  USING THE KTSA.


THE EXPANDED WORD ASSOCIATION TEST AS A MEASURE OF PSYCHOLOGICAL DEFICIT  ASSOCIATED WITH BRAIN-DAMAGE.


AN APPLICATION OF THE GRASSI BLOCK SUBSTITUTION TEST IN THE  DETERMINATION OF ORGANICITY.


THE DIAGNOSTIC USEFULNESS OF  TWO TESTS OF BRAIN-DAMAGE.


WISC SUBTEST VARIABILITY AND VALIDITY OF WISC IQ.


THE RELATIONSHIP BETWEEN PERFORMANCE MINUS VERBAL SCORES  AND EXTRAVERSION IN A SEVERELY SOCIOPATHIC POPULATION.


A SPLIT-HALF ABBREVIATION OF THE WAIS.

A SPLIT-HALF ABBREVIATION OF THE WAIS.


SOME ELECTRICAL PROPERTIES OF A NUCLEAR MEMBRANE EXAMINED WITH A MICROELECTRODE. Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 ohm cm(2) and sustains a "resting" potential, the nucleoplasm being about 15 mv negative with respect to the cytoplasm. Both the resistance and potential appear to be associated with the nuclear membrane: the potential declines to zero and the resistance to a fraction of its original value, when the membrane is perforated experimentally.


INTRASPECIES AND INTERSPECIES TRANSFORMATION REACTIONS IN PNEUMOCOCCUS AND STREPTOCOCCUS. The efficiency of transformation of pneumococcus and a strain of viridans streptococcus (strain D) to streptomycin resistance is influenced by the species in which the mutation to resistance occurred, as well as by the species in which the mutated gene has been replicated. Pneumococcus and streptococcus strain D transform in higher frequency with DNA that has been replicated in bacteria of the same species than with DNA from the heterologous species. However, the difference between the frequencies of interspecific and intraspecific transformation is much greater with pneumococcus as receptor than with streptococcus. In addition pneumococcus transforms in higher frequency with wholly homologous (pneumococcal) DNA than with DNA from pneumococci that have replicated the streptococcal Sm(r) gene. Pneumococcus is transformed in lower frequency by wholly heterologous (streptococcal) DNA than by DNA from streptococci that have replicated the pneumococcal Sm(r) gene. Streptococcus behaves similarly in that wholly homologous (streptococcal) DNA transforms it more efficiently than when the transforming fragment contains a pneumococcal moiety. Streptococcus is transformed in the same or lower frequency by wholly heterologous (pneumococcal) DNA than by DNA from pneumococci that have replicated the streptococcal Sm(r) gene. When erythromycin resistance was used as genetic marker instead of streptomycin resistance, similar results were found.


ON THE MECHANISM OF SOME TEMPERATURE EFFECTS ON DROSOPHILA. The results of detailed kinetic studies on temperature adaptation and on crossvein deformation in Drosophila melanogaster pupae are presented. A scheme which unifies most of these effects is offered, suggesting that the basic events are a series of changes in the tertiary structure of a protein. Implications of the findings and the scheme are discussed with particular respect to temperature adaptation and to development.


STRUCTURAL REQUIREMENTS FOR THE ACTION OF NEUROHYPOPHYSEAL HORMONES UPON THE ISOLATED AMPHIBIAN URINARY BLADDER. The response of the isolated amphibian urinary bladder to thirty-four structural analogs of arginine vasotocin was determined in an effort to define the physiological significance of specific structural groups on the hormone molecule. All but one of the analogs tested possessed full intrinsic activity in this system but varied greatly in their affinity for the receptor site. An analysis of the effect of changes in hydrogen ion concentration upon the response of the bladder to oxytocin was performed in order to determine the number and nature of the ionizable groups involved in hormone receptor interaction. Two ionizable groups with apparent pK's of 7.1 and 7.75 were found to be important in determining the magnitude of the hormonal response. On the basis of the results it was postulated that hormone-receptor interaction can be considered a two-step process: (a) The binding or attachment of hormone to receptor site through ionic, hydrogen, and hydrophobic bonds and (b) a disulfide interchange reaction between hormonal disulfide and receptor sulfhydryl. The latter step is considered to be the reaction which initiates the chain of events leading to the observed change in permeability.


SODIUM FLUXES IN DESHEATHED FROG SCIATIC NERVE. Desheathed frog (R. pipiens) sciatic nerves were soaked in solutions that contained ouabain or NaN(3), in K-free solutions, or in K- and Ca-free solutions. Then, the nerves were allowed to recover in standard Ringer's solution. At various times during the soaking periods, some nerves were analyzed for Na and K, and estimates were made of the influx and efflux of Na(22). While a nerve was soaking in any one of the experimental solutions, the Na(22) influx was increased, the Na content was rising, and the K content was falling by an equivalent amount. The rate coefficient for Na(22) efflux was reduced by about 25 per cent by 0.05 mM ouabain and by about 50 per cent by 5 mM NaN(3). Potassium-free solutions had little effect on the rate coefficient. It was concluded that the efflux of Na from frog nerve is dependent on the metabolism, but not on the external concentration of K. The equimolar exchange that is characteristic of the net movements of Na and K in frog nerve may not be due to the presence of a tightly coupled Na-K exchange pump, but may represent a constraint imposed by the requirement for electroneutrality.


POTASSIUM FLUXES IN DESHEATHED FROG SCIATIC NERVE. Desheathed frog (R. pipiens) sciatic nerves were soaked in Na-deficient solutions, and measurements were made of their Na and K contents and of the movements of K(42). When a nerve is in Ringer's solution, the Na fluxes are equal to the K fluxes, and about 75 per cent of the K influx is due to active transport. The Na content and the Na efflux are linearly related to the Na concentration of the bathing solution, while the K content and the K fluxes are not so related. When a nerve is in a solution in which 75 per cent of the NaCl has been replaced by choline chloride or sucrose, the active K influx exceeds the active Na efflux, and the K content is maintained. When a nerve is soaked in a solution that contains Li, the K(42) uptake is inhibited, and the nerve loses K and gains Li. When a Li-loaded nerve recovers in a Li-free solution, K is taken up in exchange for Li. This uptake of K requires Na in the external solution. It is concluded that the active transports of K and of Na may be due to different processes, that an accumulation of K occurs only in exchange for an intracellular cation, which need not be Na, and that Na plays a specific, but unknown, role in K transport.