Datasets:

TopRepo
/

Human

language:
  - en
tags:
  - proteomics
  - bioinformatics
  - mass spectrometry
  - LC-MS
  - spectral library
  - protein
  - protein identification
pretty_name: Human Dataset
license: apache-2.0

TopRepo is a top-down spectral repository containing more than 12 million MS/MS spectra from 12 species. For each MS raw file in TopRepo, msconvert is used to convert the raw file to a centroided mzML file, and then TopFD is employed to deconvolute spectra in the centroided mzML file to one or several msalign files and proteoform feature files. The msalign files are searched against its corresponding proteome sequence database for spectral identification using TopPIC. The identification results are stored in TSV files.

Using mzML files, msalign files, feature files, and spectral identification (TSV) files generated from the data analysis pipeline, Python scripts in this repository are used to generate TSV files with comprehensive spectral information, annotated msalign files, and annotated mgf files.

Dataset Structure

The dataset consists of two TSV files for the species Human.

Metadata Table toprepo_human_meta_table_v1.2.0.tsv Contains experiment-level metadata including:

dataset identifiers
species information
instrument metadata
dissociation methods
counts of spectra, proteins, and proteoforms

Spectrum Table toprepo_human_spectrum_table_ms2_v1.2.0.tsv Contains spectrum-level MS/MS analytical data including:

scan identifiers
precursor ion information
fragmentation measurements
proteoform annotations
protein identifications
statistical confidence metrics

Annotated .msalign file

Sources

Repository https://toprepo.org/
Paper https://www.biorxiv.org/content/10.64898/2026.02.20.707032v1
Github https://github.com/toppic-suite/toprepo

Contact

For questions regarding this dataset, please contact the TopRepo team through: xwliu@tulane.edu kli7@tulane.edu